Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97548.t1 | RHN44803.1 | 95.6 | 137 | 6 | 0 | 1 | 137 | 18 | 154 | 4.80E-66 | 260.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97548.t1 | A0A072TYJ6 | 95.6 | 137 | 6 | 0 | 1 | 137 | 1 | 137 | 3.4e-66 | 260.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053097 | MS.gene97548 | 0.825988 | 3.47E-54 | -1.69E-46 |
| MS.gene058171 | MS.gene97548 | 0.81785 | 2.66E-52 | -1.69E-46 |
| MS.gene058173 | MS.gene97548 | 0.820986 | 5.13E-53 | -1.69E-46 |
| MS.gene058175 | MS.gene97548 | 0.820256 | 7.55E-53 | -1.69E-46 |
| MS.gene059545 | MS.gene97548 | 0.829063 | 6.35E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97548.t1 | MTR_7g024873 | 95.620 | 137 | 6 | 0 | 1 | 137 | 1 | 137 | 4.35e-94 | 268 |
| MS.gene97548.t1 | MTR_7g024880 | 94.891 | 137 | 7 | 0 | 1 | 137 | 1 | 137 | 1.76e-92 | 263 |
| MS.gene97548.t1 | MTR_7g024890 | 94.891 | 137 | 7 | 0 | 1 | 137 | 1 | 137 | 2.42e-92 | 263 |
| MS.gene97548.t1 | MTR_8g446810 | 47.857 | 140 | 70 | 1 | 1 | 137 | 1 | 140 | 1.10e-40 | 132 |
| MS.gene97548.t1 | MTR_7g024900 | 96.078 | 51 | 2 | 0 | 1 | 51 | 1 | 51 | 8.90e-29 | 100 |
| MS.gene97548.t1 | MTR_1g081270 | 36.641 | 131 | 78 | 1 | 1 | 131 | 1 | 126 | 6.89e-20 | 79.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97548.t1 | AT2G44310 | 46.715 | 137 | 71 | 1 | 1 | 135 | 1 | 137 | 9.27e-40 | 130 |
Find 26 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGATGAGTGTAGAAATTT+TGG | 0.159822 | 7.2:+74778499 | None:intergenic |
| CTTGAATGGCACAATTGATT+TGG | 0.268459 | 7.2:+74778754 | MS.gene97548:CDS |
| TAAACACGAGCAAGCTCGTT+TGG | 0.337682 | 7.2:-74778701 | None:intergenic |
| TAAATGCTTCTTCATCTTCA+AGG | 0.379890 | 7.2:-74778544 | None:intergenic |
| CATGCCAATTCAAATGGTCT+TGG | 0.391639 | 7.2:+74778835 | MS.gene97548:CDS |
| TCTTCCAAGACCATTTGAAT+TGG | 0.395041 | 7.2:-74778839 | None:intergenic |
| GAAGCATTTAATAGCTCAGT+TGG | 0.431408 | 7.2:+74778557 | MS.gene97548:CDS |
| TTAATAGCTCAGTTGGAAAT+CGG | 0.432475 | 7.2:+74778564 | MS.gene97548:CDS |
| CTTCTTCATCTTCAAGGAAG+TGG | 0.447415 | 7.2:-74778538 | None:intergenic |
| GGGTTTCATGCCAATTCAAA+TGG | 0.458206 | 7.2:+74778829 | MS.gene97548:CDS |
| AAACACGAGCAAGCTCGTTT+GGG | 0.462526 | 7.2:-74778700 | None:intergenic |
| GAAGGAGCTCCGGAGCTTGA+GGG | 0.481338 | 7.2:+74778640 | MS.gene97548:CDS |
| CAGTTCGATCACAACTTGAA+TGG | 0.485615 | 7.2:+74778740 | MS.gene97548:CDS |
| CTCTTATGATGAGATGTTGA+AGG | 0.511648 | 7.2:+74778622 | MS.gene97548:CDS |
| GCTTGCAATGGCAGATGGGA+TGG | 0.521128 | 7.2:+74778808 | MS.gene97548:CDS |
| TGAAGGAGCTCCGGAGCTTG+AGG | 0.522904 | 7.2:+74778639 | MS.gene97548:CDS |
| ATGAGATGTTGAAGGAGCTC+CGG | 0.525969 | 7.2:+74778630 | MS.gene97548:CDS |
| TGAAAGAGAGTACAATAAAG+TGG | 0.551009 | 7.2:+74778886 | MS.gene97548:CDS |
| CTTGCAATGGCAGATGGGAT+GGG | 0.557101 | 7.2:+74778809 | MS.gene97548:CDS |
| ATGATGCTTGCAATGGCAGA+TGG | 0.558215 | 7.2:+74778803 | MS.gene97548:CDS |
| AACACACTTTGGTATTGATG+TGG | 0.581840 | 7.2:+74778670 | MS.gene97548:CDS |
| TTCAAGGAAGTGGACAATTG+TGG | 0.596143 | 7.2:-74778528 | None:intergenic |
| CGCCTCGACACAAACAGCGA+TGG | 0.598011 | 7.2:+74778593 | MS.gene97548:CDS |
| AAAGCAGATGATGCTTGCAA+TGG | 0.609930 | 7.2:+74778796 | MS.gene97548:CDS |
| GACCATCGCTGTTTGTGTCG+AGG | 0.621315 | 7.2:-74778595 | None:intergenic |
| TGATGCTTGCAATGGCAGAT+GGG | 0.625156 | 7.2:+74778804 | MS.gene97548:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TAAATGCTTCTTCATCTTCA+AGG | - | chr7.2:74778547-74778566 | None:intergenic | 30.0% | |
| TTAATAGCTCAGTTGGAAAT+CGG | + | chr7.2:74778564-74778583 | MS.gene97548:CDS | 30.0% | |
| ! | ATGAGTGTAGAAATTTTGGA+TGG | + | chr7.2:74778503-74778522 | MS.gene97548:CDS | 30.0% |
| ! | TGAAAGAGAGTACAATAAAG+TGG | + | chr7.2:74778886-74778905 | MS.gene97548:CDS | 30.0% |
| CTCTTATGATGAGATGTTGA+AGG | + | chr7.2:74778622-74778641 | MS.gene97548:CDS | 35.0% | |
| CTTGAATGGCACAATTGATT+TGG | + | chr7.2:74778754-74778773 | MS.gene97548:CDS | 35.0% | |
| TCTTCCAAGACCATTTGAAT+TGG | - | chr7.2:74778842-74778861 | None:intergenic | 35.0% | |
| ! | GAAGCATTTAATAGCTCAGT+TGG | + | chr7.2:74778557-74778576 | MS.gene97548:CDS | 35.0% |
| !! | AACACACTTTGGTATTGATG+TGG | + | chr7.2:74778670-74778689 | MS.gene97548:CDS | 35.0% |
| !! | CTCTTTCAACAGCTATTTTG+AGG | - | chr7.2:74778874-74778893 | None:intergenic | 35.0% |
| !!! | AGGGTTTTTGAAACACACTT+TGG | + | chr7.2:74778659-74778678 | MS.gene97548:CDS | 35.0% |
| AAAGCAGATGATGCTTGCAA+TGG | + | chr7.2:74778796-74778815 | MS.gene97548:CDS | 40.0% | |
| CAGTTCGATCACAACTTGAA+TGG | + | chr7.2:74778740-74778759 | MS.gene97548:CDS | 40.0% | |
| CATGCCAATTCAAATGGTCT+TGG | + | chr7.2:74778835-74778854 | MS.gene97548:CDS | 40.0% | |
| CTTCTTCATCTTCAAGGAAG+TGG | - | chr7.2:74778541-74778560 | None:intergenic | 40.0% | |
| GGGTTTCATGCCAATTCAAA+TGG | + | chr7.2:74778829-74778848 | MS.gene97548:CDS | 40.0% | |
| TTCAAGGAAGTGGACAATTG+TGG | - | chr7.2:74778531-74778550 | None:intergenic | 40.0% | |
| TTTCAAAAACCCTCAAGCTC+CGG | - | chr7.2:74778652-74778671 | None:intergenic | 40.0% | |
| AAACACGAGCAAGCTCGTTT+GGG | - | chr7.2:74778703-74778722 | None:intergenic | 45.0% | |
| ATGAGATGTTGAAGGAGCTC+CGG | + | chr7.2:74778630-74778649 | MS.gene97548:CDS | 45.0% | |
| ATGATGCTTGCAATGGCAGA+TGG | + | chr7.2:74778803-74778822 | MS.gene97548:CDS | 45.0% | |
| TAAACACGAGCAAGCTCGTT+TGG | - | chr7.2:74778704-74778723 | None:intergenic | 45.0% | |
| TGATGCTTGCAATGGCAGAT+GGG | + | chr7.2:74778804-74778823 | MS.gene97548:CDS | 45.0% | |
| CTTGCAATGGCAGATGGGAT+GGG | + | chr7.2:74778809-74778828 | MS.gene97548:CDS | 50.0% | |
| GACCATCGCTGTTTGTGTCG+AGG | - | chr7.2:74778598-74778617 | None:intergenic | 55.0% | |
| GCTTGCAATGGCAGATGGGA+TGG | + | chr7.2:74778808-74778827 | MS.gene97548:CDS | 55.0% | |
| CGCCTCGACACAAACAGCGA+TGG | + | chr7.2:74778593-74778612 | MS.gene97548:CDS | 60.0% | |
| GAAGGAGCTCCGGAGCTTGA+GGG | + | chr7.2:74778640-74778659 | MS.gene97548:CDS | 60.0% | |
| TGAAGGAGCTCCGGAGCTTG+AGG | + | chr7.2:74778639-74778658 | MS.gene97548:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 74778503 | 74778916 | 74778503 | ID=MS.gene97548 |
| chr7.2 | mRNA | 74778503 | 74778916 | 74778503 | ID=MS.gene97548.t1;Parent=MS.gene97548 |
| chr7.2 | exon | 74778503 | 74778916 | 74778503 | ID=MS.gene97548.t1.exon1;Parent=MS.gene97548.t1 |
| chr7.2 | CDS | 74778503 | 74778916 | 74778503 | ID=cds.MS.gene97548.t1;Parent=MS.gene97548.t1 |
| Gene Sequence |
| Protein sequence |
