Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97687.t1 | XP_013467617.1 | 96.8 | 539 | 17 | 0 | 1 | 539 | 1 | 539 | 4.60E-298 | 1033.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97687.t1 | Q640P7 | 22.8 | 487 | 330 | 12 | 65 | 538 | 80 | 533 | 1.9e-26 | 122.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97687.t1 | A0A072VHZ6 | 96.8 | 539 | 17 | 0 | 1 | 539 | 1 | 539 | 3.3e-298 | 1033.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049321 | MS.gene97687 | 0.805248 | 1.46E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97687.t1 | MTR_1g053360 | 96.846 | 539 | 17 | 0 | 1 | 539 | 1 | 539 | 0.0 | 1085 |
| MS.gene97687.t1 | MTR_7g092810 | 77.947 | 526 | 112 | 3 | 15 | 539 | 22 | 544 | 0.0 | 854 |
| MS.gene97687.t1 | MTR_6g045280 | 66.129 | 62 | 21 | 0 | 234 | 295 | 1 | 62 | 4.26e-22 | 92.8 |
| MS.gene97687.t1 | MTR_7g078290 | 78.000 | 50 | 10 | 1 | 147 | 196 | 58 | 106 | 1.18e-15 | 73.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97687.t1 | AT3G57890 | 68.333 | 540 | 160 | 4 | 8 | 539 | 23 | 559 | 0.0 | 773 |
| MS.gene97687.t1 | AT3G57890 | 64.062 | 576 | 160 | 5 | 8 | 539 | 23 | 595 | 0.0 | 753 |
| MS.gene97687.t1 | AT2G42230 | 66.792 | 533 | 168 | 3 | 15 | 539 | 23 | 554 | 0.0 | 750 |
| MS.gene97687.t1 | AT2G42230 | 67.254 | 397 | 121 | 3 | 15 | 403 | 23 | 418 | 0.0 | 555 |
Find 137 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGATGCTCACCTGCAATTTA+TGG | 0.177659 | 1.1:-39754675 | None:intergenic |
| AGGACTTCTGCTAAGGAAAA+TGG | 0.208783 | 1.1:+39751846 | MS.gene97687:CDS |
| TTAAGATATGCCATTGTCTA+TGG | 0.241107 | 1.1:+39753374 | MS.gene97687:CDS |
| CCTTCTACTTCTGCGTTTGA+TGG | 0.259375 | 1.1:+39749387 | MS.gene97687:CDS |
| CTCTGCTCTCTCTCCTCTTC+AGG | 0.274169 | 1.1:+39749416 | MS.gene97687:CDS |
| CACTCATGTGCTCCTCCAAT+TGG | 0.275744 | 1.1:-39755482 | None:intergenic |
| TTCTCCTTTCTTTGCAAATT+CGG | 0.277718 | 1.1:+39751731 | MS.gene97687:CDS |
| CTGCTTCTGTTGCTGATGTT+TGG | 0.283989 | 1.1:+39749364 | MS.gene97687:CDS |
| GGATCGGAGAAGATTAGTTT+TGG | 0.302161 | 1.1:-39749087 | None:intergenic |
| GCAAAATATATCTTCTGCTT+TGG | 0.313310 | 1.1:+39751809 | MS.gene97687:CDS |
| ACTCATGTGCTCCTCCAATT+GGG | 0.320118 | 1.1:-39755481 | None:intergenic |
| GACTCTCCTGCGAGCCAGTT+TGG | 0.320305 | 1.1:-39755934 | None:intergenic |
| TAGACAATGGCATATCTTAA+AGG | 0.325112 | 1.1:-39753371 | None:intergenic |
| CAGTCATGAACTTGAGTAAC+AGG | 0.328107 | 1.1:-39751780 | None:intergenic |
| CATAAAAGGTTCCTCTGTTA+AGG | 0.328768 | 1.1:+39752004 | MS.gene97687:CDS |
| AAGCATGCATCTGACATAAA+AGG | 0.335471 | 1.1:+39751990 | MS.gene97687:CDS |
| AAAATAATTTAGGAGAAATA+AGG | 0.336429 | 1.1:+39756112 | MS.gene97687:CDS |
| TCTAGCCGAGCCAGGGGAGG+AGG | 0.341097 | 1.1:+39750694 | MS.gene97687:CDS |
| ACAAAATTTGTGAACTGATC+AGG | 0.347132 | 1.1:-39755636 | None:intergenic |
| TACTTCAAGGACTGGCTATA+TGG | 0.349727 | 1.1:+39756201 | MS.gene97687:CDS |
| AGTGAACCAGCAACCTCTTA+TGG | 0.370913 | 1.1:+39754642 | MS.gene97687:CDS |
| ACGGGAACCGCGGGCATCTC+AGG | 0.374675 | 1.1:-39751756 | None:intergenic |
| CCTAGTTCAGTTTGGCGATA+AGG | 0.381659 | 1.1:+39751683 | MS.gene97687:CDS |
| TCAGATGCTACAATAGTTCT+TGG | 0.382767 | 1.1:+39753401 | MS.gene97687:CDS |
| GAAAACGACGTCGTCGTTGA+TGG | 0.388498 | 1.1:+39749258 | MS.gene97687:CDS |
| GGAGATGAGGTGGTTGGAGG+AGG | 0.395910 | 1.1:-39749151 | None:intergenic |
| TGGATAGATTTGAGCACCTT+GGG | 0.396913 | 1.1:+39751658 | MS.gene97687:CDS |
| TATGCATCTGGTAATGCAAA+TGG | 0.406433 | 1.1:-39755979 | None:intergenic |
| AACATCTTGATCAGCGGAAC+TGG | 0.421388 | 1.1:-39751874 | None:intergenic |
| CTTGGGTTCCTAGTTCAGTT+TGG | 0.422155 | 1.1:+39751675 | MS.gene97687:CDS |
| TGGTTATCACCAACCATAAG+AGG | 0.423877 | 1.1:-39754655 | None:intergenic |
| GAACAGATGGGATGAAGCTC+TGG | 0.425086 | 1.1:+39755527 | MS.gene97687:CDS |
| TTTGTCTGCAGATTCCAAAC+TGG | 0.435268 | 1.1:+39755920 | MS.gene97687:intron |
| AGATTTAGTGATCCCTTCAA+TGG | 0.439889 | 1.1:-39751958 | None:intergenic |
| AAGGAGGAGTTGAGCGTGTT+CGG | 0.445661 | 1.1:-39749208 | None:intergenic |
| CTATCTCTTCTAGCCGAGCC+AGG | 0.445990 | 1.1:+39750686 | MS.gene97687:CDS |
| AGCCGAGCCAGGGGAGGAGG+GGG | 0.446711 | 1.1:+39750697 | MS.gene97687:CDS |
| CCTTATCGCCAAACTGAACT+AGG | 0.447758 | 1.1:-39751683 | None:intergenic |
| TTCGCGGCGTGGGTGGATCA+TGG | 0.453267 | 1.1:-39749037 | None:intergenic |
| ACAATAGTTCTTGGAGCAGT+TGG | 0.453677 | 1.1:+39753410 | MS.gene97687:CDS |
| TGTGCTTCATGTGTACTTCA+AGG | 0.453994 | 1.1:+39756188 | MS.gene97687:CDS |
| GCTCGAATGGTTCGCGGCGT+GGG | 0.455676 | 1.1:-39749047 | None:intergenic |
| AGCCGAGTCTGCTACACGCC+TGG | 0.458775 | 1.1:+39755611 | MS.gene97687:CDS |
| CACCAACCATAAGAGGTTGC+TGG | 0.458926 | 1.1:-39754648 | None:intergenic |
| GGAGGAGATGAGGTGGTTGG+AGG | 0.459524 | 1.1:-39749154 | None:intergenic |
| GGACTTCTGCTAAGGAAAAT+GGG | 0.460054 | 1.1:+39751847 | MS.gene97687:CDS |
| GTTGGAGGAGGAAGCGAGGA+GGG | 0.462877 | 1.1:-39749139 | None:intergenic |
| GGTGGTTGGAGGAGGAAGCG+AGG | 0.470413 | 1.1:-39749143 | None:intergenic |
| AGGGATTCATGTTCCATTGA+AGG | 0.471417 | 1.1:+39751945 | MS.gene97687:CDS |
| TTGTGAACTGATCAGGGTCC+AGG | 0.471481 | 1.1:-39755629 | None:intergenic |
| CCATCAAACGCAGAAGTAGA+AGG | 0.472003 | 1.1:-39749387 | None:intergenic |
| GAGGAGCACATGAGTGAAGT+TGG | 0.474276 | 1.1:+39755489 | MS.gene97687:CDS |
| AGGTTCTATTGGTTCTGTCA+TGG | 0.479874 | 1.1:-39748995 | None:intergenic |
| TGCTCGAATGGTTCGCGGCG+TGG | 0.481917 | 1.1:-39749048 | None:intergenic |
| TAAAGGTGCTAGGATATAGA+TGG | 0.484265 | 1.1:-39753354 | None:intergenic |
| CTCATGTGCTCCTCCAATTG+GGG | 0.484387 | 1.1:-39755480 | None:intergenic |
| GTCCAGGCGTGTAGCAGACT+CGG | 0.487472 | 1.1:-39755613 | None:intergenic |
| CTGTTACTCAAGTTCATGAC+TGG | 0.488554 | 1.1:+39751781 | MS.gene97687:CDS |
| AGCTTATGCGCAGCAATAGT+CGG | 0.490223 | 1.1:+39750591 | MS.gene97687:intron |
| GAAGAGATAGAATGTTAGCC+AGG | 0.494110 | 1.1:-39750673 | None:intergenic |
| TGAGACGCCGCATATGCATC+TGG | 0.494292 | 1.1:-39755991 | None:intergenic |
| GGGATTAGGCCGTGCTCGAA+TGG | 0.495534 | 1.1:-39749060 | None:intergenic |
| CTAGCCGAGCCAGGGGAGGA+GGG | 0.500416 | 1.1:+39750695 | MS.gene97687:CDS |
| TGTGATGTGGAAGGTTCTAT+TGG | 0.502246 | 1.1:-39749006 | None:intergenic |
| GGATATAGATGGTAGACTCT+TGG | 0.502963 | 1.1:-39753343 | None:intergenic |
| TGGTGATAACCATAAATTGC+AGG | 0.503356 | 1.1:+39754666 | MS.gene97687:CDS |
| CGGCGTGGGTGGATCATGGT+GGG | 0.507249 | 1.1:-39749033 | None:intergenic |
| TTCTGCCCACAGTGAACAGA+TGG | 0.508686 | 1.1:+39755514 | MS.gene97687:CDS |
| TAGTGTGAGGAGGGATTGTG+TGG | 0.510275 | 1.1:-39749109 | None:intergenic |
| CAGATTCCAAACTGGCTCGC+AGG | 0.511021 | 1.1:+39755928 | MS.gene97687:intron |
| CAAACATCAGCAACAGAAGC+AGG | 0.512025 | 1.1:-39749363 | None:intergenic |
| CGAATCGGAGATTGTGGCGG+AGG | 0.512705 | 1.1:-39749172 | None:intergenic |
| TTCTGAAAGGACTTCTGCTA+AGG | 0.512893 | 1.1:+39751839 | MS.gene97687:CDS |
| TCTTGATCAGCGGAACTGGC+TGG | 0.515761 | 1.1:-39751870 | None:intergenic |
| GGCGGAGGAGATGAGGTGGT+TGG | 0.518669 | 1.1:-39749157 | None:intergenic |
| CAAAATTTGTGAACTGATCA+GGG | 0.519211 | 1.1:-39755635 | None:intergenic |
| GGATCCGAATTTGCAAAGAA+AGG | 0.520206 | 1.1:-39751735 | None:intergenic |
| GAGGAGGGATTGTGTGGGAT+CGG | 0.522926 | 1.1:-39749103 | None:intergenic |
| GAAAACACATTCACGGCAGT+TGG | 0.523649 | 1.1:-39754611 | None:intergenic |
| GGCCGTGCTCGAATGGTTCG+CGG | 0.523853 | 1.1:-39749053 | None:intergenic |
| CTTTGGAACATATTTCTGAA+AGG | 0.524901 | 1.1:+39751826 | MS.gene97687:CDS |
| GACTGATGCATGCTCTGTCT+CGG | 0.529353 | 1.1:+39751902 | MS.gene97687:CDS |
| TCATCCCATCTGTTCACTGT+GGG | 0.531454 | 1.1:-39755519 | None:intergenic |
| TTAAGTTGTCATCAAGTACC+AGG | 0.537403 | 1.1:+39751925 | MS.gene97687:CDS |
| TAGCCGAGCCAGGGGAGGAG+GGG | 0.538350 | 1.1:+39750696 | MS.gene97687:CDS |
| ATGGATAGATTTGAGCACCT+TGG | 0.539243 | 1.1:+39751657 | MS.gene97687:CDS |
| GCAAAAGCACGCACCTGAAG+AGG | 0.539506 | 1.1:-39749429 | None:intergenic |
| GGGATTCATGTTCCATTGAA+GGG | 0.539534 | 1.1:+39751946 | MS.gene97687:CDS |
| TTCAATGGAACATGAATCCC+TGG | 0.541433 | 1.1:-39751943 | None:intergenic |
| GTCTTATCTGCAAAAGCACC+TGG | 0.542117 | 1.1:+39750655 | MS.gene97687:CDS |
| GCCTCTTCATCAGCCTGCGA+TGG | 0.542685 | 1.1:-39750623 | None:intergenic |
| CTGGCTCGCAGGAGAGTCCA+CGG | 0.547439 | 1.1:+39755939 | MS.gene97687:CDS |
| GGTTGGAGGAGGAAGCGAGG+AGG | 0.551379 | 1.1:-39749140 | None:intergenic |
| CGAATGGTTCGCGGCGTGGG+TGG | 0.551551 | 1.1:-39749044 | None:intergenic |
| GGTTGAGATCTGAAGCGAAT+CGG | 0.556748 | 1.1:-39749187 | None:intergenic |
| CTGAAGCGAATCGGAGATTG+TGG | 0.559351 | 1.1:-39749178 | None:intergenic |
| TGAACTTGAGTAACAGGGAC+GGG | 0.564622 | 1.1:-39751774 | None:intergenic |
| GATTCATTATCTCATCCAGC+TGG | 0.570600 | 1.1:+39755573 | MS.gene97687:CDS |
| GCCATCGCAGGCTGATGAAG+AGG | 0.572306 | 1.1:+39750622 | MS.gene97687:CDS |
| CAGTTTGGCGATAAGGCTGA+AGG | 0.573370 | 1.1:+39751690 | MS.gene97687:CDS |
| AGATTGTGGCGGAGGAGATG+AGG | 0.574003 | 1.1:-39749164 | None:intergenic |
| ATGCATCTGGTAATGCAAAT+GGG | 0.576674 | 1.1:-39755978 | None:intergenic |
| TATCTCTTCTAGCCGAGCCA+GGG | 0.577420 | 1.1:+39750687 | MS.gene97687:CDS |
| TGAACATCAGAGACACCAGC+TGG | 0.580335 | 1.1:-39755588 | None:intergenic |
| GCGGCGTGGGTGGATCATGG+TGG | 0.586738 | 1.1:-39749034 | None:intergenic |
| TTCATGTGTACTTCAAGGAC+TGG | 0.590213 | 1.1:+39756193 | MS.gene97687:CDS |
| TGCATTACCAGATGCATATG+CGG | 0.590787 | 1.1:+39755984 | MS.gene97687:CDS |
| TCACGGAGGAGAGAGTATCA+AGG | 0.592286 | 1.1:-39749227 | None:intergenic |
| CGCCGCGAACCATTCGAGCA+CGG | 0.594832 | 1.1:+39749051 | MS.gene97687:CDS |
| TGGCATATCTTAAAGGTGCT+AGG | 0.596062 | 1.1:-39753364 | None:intergenic |
| TCTTCTAGCCGAGCCAGGGG+AGG | 0.600027 | 1.1:+39750691 | MS.gene97687:CDS |
| GTAACAGGGACGGGAACCGC+GGG | 0.601270 | 1.1:-39751765 | None:intergenic |
| TTCATCCCATCTGTTCACTG+TGG | 0.601962 | 1.1:-39755520 | None:intergenic |
| AACCAGCAACCTCTTATGGT+TGG | 0.603151 | 1.1:+39754646 | MS.gene97687:CDS |
| AGTAACAGGGACGGGAACCG+CGG | 0.603705 | 1.1:-39751766 | None:intergenic |
| AGTTCTTGGAGCAGTTGGCA+AGG | 0.610594 | 1.1:+39753415 | MS.gene97687:CDS |
| CATGGTGGGAGCGTGTGATG+TGG | 0.614824 | 1.1:-39749019 | None:intergenic |
| GGATGAGATAATGAATCATG+AGG | 0.623920 | 1.1:-39755567 | None:intergenic |
| CAAACTGAACTAGGAACCCA+AGG | 0.625171 | 1.1:-39751674 | None:intergenic |
| TGAAAGAGTTCATGTGATTG+TGG | 0.625475 | 1.1:+39754567 | MS.gene97687:CDS |
| ATCTCTTCTAGCCGAGCCAG+GGG | 0.631243 | 1.1:+39750688 | MS.gene97687:CDS |
| AGTGTGAGGAGGGATTGTGT+GGG | 0.633725 | 1.1:-39749108 | None:intergenic |
| TGGCTCGCAGGAGAGTCCAC+GGG | 0.633963 | 1.1:+39755940 | MS.gene97687:CDS |
| GTGGGAGCGTGTGATGTGGA+AGG | 0.635216 | 1.1:-39749015 | None:intergenic |
| ATCTGAGCATCCATAGACAA+TGG | 0.637689 | 1.1:-39753384 | None:intergenic |
| TCATGTGCTCCTCCAATTGG+GGG | 0.639194 | 1.1:-39755479 | None:intergenic |
| TTGTGGCGGAGGAGATGAGG+TGG | 0.642507 | 1.1:-39749161 | None:intergenic |
| TCTGCCCACAGTGAACAGAT+GGG | 0.648990 | 1.1:+39755515 | MS.gene97687:CDS |
| TTGGAAACAAACCTTAACAG+AGG | 0.661782 | 1.1:-39752015 | None:intergenic |
| TAAGTTGTCATCAAGTACCA+GGG | 0.662209 | 1.1:+39751926 | MS.gene97687:CDS |
| ATGAACTTGAGTAACAGGGA+CGG | 0.665587 | 1.1:-39751775 | None:intergenic |
| CATAGCAACATCTTGATCAG+CGG | 0.675580 | 1.1:-39751880 | None:intergenic |
| AAGCGAATCGGAGATTGTGG+CGG | 0.676999 | 1.1:-39749175 | None:intergenic |
| CGGAGGAGAGAGTATCAAGG+AGG | 0.705309 | 1.1:-39749224 | None:intergenic |
| TTCGGATCCTGAGATGCCCG+CGG | 0.705467 | 1.1:+39751749 | MS.gene97687:CDS |
| GCAGGATCTTTATTCGAACG+CGG | 0.706027 | 1.1:-39749345 | None:intergenic |
| AGTCATGAACTTGAGTAACA+GGG | 0.713975 | 1.1:-39751779 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 39748997 | 39756222 | 39748997 | ID=MS.gene97687 |
| chr1.1 | mRNA | 39748997 | 39756222 | 39748997 | ID=MS.gene97687.t1;Parent=MS.gene97687 |
| chr1.1 | exon | 39748997 | 39749437 | 39748997 | ID=MS.gene97687.t1.exon1;Parent=MS.gene97687.t1 |
| chr1.1 | CDS | 39748997 | 39749437 | 39748997 | ID=cds.MS.gene97687.t1;Parent=MS.gene97687.t1 |
| chr1.1 | exon | 39750593 | 39750736 | 39750593 | ID=MS.gene97687.t1.exon2;Parent=MS.gene97687.t1 |
| chr1.1 | CDS | 39750593 | 39750736 | 39750593 | ID=cds.MS.gene97687.t1;Parent=MS.gene97687.t1 |
| chr1.1 | exon | 39751648 | 39752025 | 39751648 | ID=MS.gene97687.t1.exon3;Parent=MS.gene97687.t1 |
| chr1.1 | CDS | 39751648 | 39752025 | 39751648 | ID=cds.MS.gene97687.t1;Parent=MS.gene97687.t1 |
| chr1.1 | exon | 39753332 | 39753436 | 39753332 | ID=MS.gene97687.t1.exon4;Parent=MS.gene97687.t1 |
| chr1.1 | CDS | 39753332 | 39753436 | 39753332 | ID=cds.MS.gene97687.t1;Parent=MS.gene97687.t1 |
| chr1.1 | exon | 39754547 | 39754687 | 39754547 | ID=MS.gene97687.t1.exon5;Parent=MS.gene97687.t1 |
| chr1.1 | CDS | 39754547 | 39754687 | 39754547 | ID=cds.MS.gene97687.t1;Parent=MS.gene97687.t1 |
| chr1.1 | exon | 39755456 | 39755659 | 39755456 | ID=MS.gene97687.t1.exon6;Parent=MS.gene97687.t1 |
| chr1.1 | CDS | 39755456 | 39755659 | 39755456 | ID=cds.MS.gene97687.t1;Parent=MS.gene97687.t1 |
| chr1.1 | exon | 39755931 | 39756023 | 39755931 | ID=MS.gene97687.t1.exon7;Parent=MS.gene97687.t1 |
| chr1.1 | CDS | 39755931 | 39756023 | 39755931 | ID=cds.MS.gene97687.t1;Parent=MS.gene97687.t1 |
| chr1.1 | exon | 39756111 | 39756222 | 39756111 | ID=MS.gene97687.t1.exon8;Parent=MS.gene97687.t1 |
| chr1.1 | CDS | 39756111 | 39756222 | 39756111 | ID=cds.MS.gene97687.t1;Parent=MS.gene97687.t1 |
| Gene Sequence |
| Protein sequence |
