AlfalfaGEDB | MS.gene049321

M. sativa cultivar XinJiangDaYe / MS.gene049321

KEGG: K10355
GO: -
PFAM: PF00022.22

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene049321.t1	XP_012571874.1	100	377	0	0	1	377	1	377	4.80E-217	763.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene049321.t1	P53492	98.4	377	6	0	1	377	1	377	4.1e-217	755.0

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene049321.t1	G7JAX4	100.0	377	0	0	1	377	1	377	3.4e-217	763.5

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049321	MS.gene049343	-0.818239	2.17E-52	-1.69E-46
MS.gene049321	MS.gene04995	0.817173	3.78E-52	-1.69E-46
MS.gene049321	MS.gene050287	0.8076	4.67E-50	-1.69E-46
MS.gene049321	MS.gene057071	-0.804829	1.79E-49	-1.69E-46
MS.gene049321	MS.gene061523	0.804343	2.26E-49	-1.69E-46
MS.gene049321	MS.gene06446	0.981221	4.58E-152	-1.69E-46
MS.gene049321	MS.gene07141	-0.812541	4.03E-51	-1.69E-46
MS.gene049321	MS.gene07145	-0.819748	9.87E-53	-1.69E-46
MS.gene049321	MS.gene24710	0.805023	1.63E-49	-1.69E-46
MS.gene049321	MS.gene31004	0.972772	2.58E-135	-1.69E-46
MS.gene049321	MS.gene31022	-0.816783	4.63E-52	-1.69E-46
MS.gene049321	MS.gene36022	0.827797	1.28E-54	-1.69E-46
MS.gene049321	MS.gene39376	0.801862	7.37E-49	-1.69E-46
MS.gene049321	MS.gene39695	0.801367	9.31E-49	-1.69E-46
MS.gene049321	MS.gene63793	-0.814619	1.40E-51	-1.69E-46
MS.gene049321	MS.gene68002	-0.802065	6.70E-49	-1.69E-46
MS.gene049321	MS.gene71945	-0.827137	1.85E-54	-1.69E-46
MS.gene049321	MS.gene73459	0.809034	2.31E-50	-1.69E-46
MS.gene049321	MS.gene73498	-0.803428	3.50E-49	-1.69E-46
MS.gene049321	MS.gene76244	-0.802276	6.06E-49	-1.69E-46
MS.gene049321	MS.gene77185	0.81017	1.32E-50	-1.69E-46
MS.gene049321	MS.gene84940	0.83829	3.14E-57	-1.69E-46
MS.gene049321	MS.gene88895	0.825549	4.41E-54	-1.69E-46
MS.gene049321	MS.gene97687	0.805248	1.46E-49	-1.69E-46

PPI:

Gene1	Gene2	Type
MS.gene049321	MS.gene053110	PPI
MS.gene049321	MS.gene037838	PPI
MS.gene31154	MS.gene049321	PPI
MS.gene044849	MS.gene049321	PPI
MS.gene049321	MS.gene055991	PPI
MS.gene049321	MS.gene37274	PPI
MS.gene055437	MS.gene049321	PPI
MS.gene049321	MS.gene014281	PPI
MS.gene049321	MS.gene065414	PPI
MS.gene033590	MS.gene049321	PPI
MS.gene049321	MS.gene032698	PPI
MS.gene049321	MS.gene86676	PPI
MS.gene049321	MS.gene04628	PPI
MS.gene049321	MS.gene92731	PPI
MS.gene049321	MS.gene024155	PPI
MS.gene049321	MS.gene052120	PPI
MS.gene82765	MS.gene049321	PPI
MS.gene049321	MS.gene77958	PPI
MS.gene049321	MS.gene55068	PPI
MS.gene049321	MS.gene52664	PPI
MS.gene034501	MS.gene049321	PPI
MS.gene049321	MS.gene022650	PPI
MS.gene049321	MS.gene30959	PPI
MS.gene049321	MS.gene68222	PPI
MS.gene049321	MS.gene45493	PPI
MS.gene049321	MS.gene37081	PPI
MS.gene049321	MS.gene001874	PPI
MS.gene45491	MS.gene049321	PPI
MS.gene049321	MS.gene86783	PPI
MS.gene049321	MS.gene46685	PPI
MS.gene049321	MS.gene063165	PPI
MS.gene017992	MS.gene049321	PPI
MS.gene064894	MS.gene049321	PPI
MS.gene049321	MS.gene000108	PPI
MS.gene79962	MS.gene049321	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene049321.t1	MTR_3g095530	100.000	377	0	0	1	377	1	377	0.0	783
MS.gene049321.t1	MTR_0015s0130	95.756	377	16	0	1	377	1	377	0.0	763
MS.gene049321.t1	MTR_7g026230	96.021	377	15	0	1	377	1	377	0.0	762
MS.gene049321.t1	MTR_7g093260	95.756	377	16	0	1	377	1	377	0.0	761
MS.gene049321.t1	MTR_6g004000	95.491	377	17	0	1	377	1	377	0.0	761
MS.gene049321.t1	MTR_2g096840	94.960	377	19	0	1	377	1	377	0.0	761
MS.gene049321.t1	MTR_2g008050	94.695	377	20	0	1	377	1	377	0.0	754
MS.gene049321.t1	MTR_7g027185	43.799	379	199	3	10	374	7	385	3.83e-121	355
MS.gene049321.t1	MTR_7g027185	45.070	355	182	2	10	351	7	361	1.94e-118	348
MS.gene049321.t1	MTR_7g027185	48.208	307	157	1	10	314	7	313	9.99e-111	326
MS.gene049321.t1	MTR_5g066400	37.360	447	206	12	1	376	1	444	7.43e-91	280
MS.gene049321.t1	MTR_8g464900	36.659	431	200	11	1	361	1	428	3.17e-84	263
MS.gene049321.t1	MTR_5g066400	34.753	446	191	12	1	376	1	416	4.03e-78	246
MS.gene049321.t1	MTR_8g089630	34.474	409	222	8	10	372	10	418	4.24e-72	231
MS.gene049321.t1	MTR_3g030550	37.105	380	213	9	7	377	1	363	2.05e-68	220
MS.gene049321.t1	MTR_5g066400	34.000	400	193	11	1	332	1	397	1.22e-66	217
MS.gene049321.t1	MTR_2g445210	28.132	423	238	7	8	366	6	426	1.50e-55	189
MS.gene049321.t1	MTR_8g007440	33.206	262	152	5	77	319	9	266	4.35e-44	154
MS.gene049321.t1	MTR_2g445210	26.882	372	206	7	8	315	6	375	6.14e-42	151
MS.gene049321.t1	MTR_3g010170	30.976	297	181	9	91	371	179	467	1.26e-39	147
MS.gene049321.t1	MTR_8g070520	32.082	293	172	10	91	366	187	469	3.90e-37	140
MS.gene049321.t1	MTR_8g070520	31.655	278	164	9	91	352	187	454	1.44e-33	130
MS.gene049321.t1	MTR_3g067945	30.556	252	156	7	9	245	21	268	8.01e-24	103
MS.gene049321.t1	MTR_3g067945	34.426	122	77	2	250	368	586	707	6.27e-16	79.7
MS.gene049321.t1	MTR_8g008540	72.973	37	10	0	340	376	41	77	2.58e-13	65.1

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene049321.t1	AT5G09810	98.408	377	6	0	1	377	1	377	0.0	774
MS.gene049321.t1	AT3G53750	95.491	377	17	0	1	377	1	377	0.0	760
MS.gene049321.t1	AT3G53750	95.491	377	17	0	1	377	1	377	0.0	760
MS.gene049321.t1	AT2G37620	95.491	377	17	0	1	377	1	377	0.0	760
MS.gene049321.t1	AT2G37620	95.491	377	17	0	1	377	1	377	0.0	760
MS.gene049321.t1	AT2G37620	95.491	377	17	0	1	377	1	377	0.0	760
MS.gene049321.t1	AT2G37620	95.491	377	17	0	1	377	1	377	0.0	760
MS.gene049321.t1	AT3G12110	95.491	377	17	0	1	377	1	377	0.0	760
MS.gene049321.t1	AT3G46520	94.430	377	21	0	1	377	1	377	0.0	749
MS.gene049321.t1	AT3G46520	94.430	377	21	0	1	377	1	377	0.0	749
MS.gene049321.t1	AT1G49240	93.369	377	25	0	1	377	1	377	0.0	749
MS.gene049321.t1	AT5G59370	94.164	377	22	0	1	377	1	377	0.0	748
MS.gene049321.t1	AT5G59370	94.164	377	22	0	1	377	1	377	0.0	748
MS.gene049321.t1	AT3G18780	93.103	377	26	0	1	377	1	377	0.0	747
MS.gene049321.t1	AT3G18780	93.103	377	26	0	1	377	1	377	0.0	747
MS.gene049321.t1	AT3G18780	92.521	361	27	0	1	361	1	361	0.0	707
MS.gene049321.t1	AT2G42100	84.511	368	57	0	10	377	11	378	0.0	674
MS.gene049321.t1	AT2G42090	71.622	370	100	1	7	376	1	365	0.0	569
MS.gene049321.t1	AT2G42170	78.313	332	69	1	46	377	1	329	0.0	560
MS.gene049321.t1	AT2G42170	78.313	332	69	1	46	377	1	329	0.0	560
MS.gene049321.t1	AT2G42170	78.526	312	64	1	46	357	1	309	0.0	523
MS.gene049321.t1	AT2G42170	78.526	312	64	1	46	357	1	309	0.0	523
MS.gene049321.t1	AT2G42170	78.319	226	46	1	152	377	9	231	2.42e-131	375
MS.gene049321.t1	AT3G27000	44.591	379	196	4	10	374	7	385	1.12e-122	360
MS.gene049321.t1	AT1G18450	38.202	445	201	11	1	376	1	440	8.62e-95	290
MS.gene049321.t1	AT1G13180	34.867	413	215	10	10	372	9	417	1.55e-72	233
MS.gene049321.t1	AT3G60830	37.467	379	213	9	7	377	1	363	1.66e-66	215
MS.gene049321.t1	AT3G33520	30.404	421	235	7	10	377	5	420	3.61e-59	197
MS.gene049321.t1	AT5G56180	30.928	291	175	9	91	364	50	331	2.01e-35	132
MS.gene049321.t1	AT5G56180	30.928	291	175	9	91	364	182	463	1.17e-34	133
MS.gene049321.t1	AT3G12380	29.572	257	158	6	9	245	21	274	3.50e-26	110
MS.gene049321.t1	AT3G12380	34.426	122	77	1	250	368	595	716	6.04e-16	80.1
MS.gene049321.t1	AT3G12380	29.412	255	155	6	9	245	21	268	1.77e-25	108
MS.gene049321.t1	AT3G12380	34.426	122	77	1	250	368	589	710	5.80e-16	80.1
MS.gene049321.t1	AT1G73910	35.862	145	74	3	1	129	1	142	2.69e-23	95.1
MS.gene049321.t1	AT1G73910	35.862	145	74	3	1	129	1	142	2.69e-23	95.1
MS.gene049321.t1	AT5G56180	31.683	202	115	7	91	281	182	371	4.62e-19	88.2
MS.gene049321.t1	AT1G73910	29.268	123	71	2	1	107	1	123	2.86e-12	65.1

Find 119 sgRNAs with CRISPR-Local

Find 134 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGAAAGTCTCAAACATGATT+TGG	0.224034	3.1:+76204590	None:intergenic
AGAAGCACTGGGTGCTCTTC+AGG	0.266274	3.1:+76204658	None:intergenic
GAGAGTAAGAATACCTCTTT+TGG	0.276320	3.1:+76204771	None:intergenic
GATGGTTGGAAAAGGACTTC+TGG	0.306272	3.1:+76204096	None:intergenic
AGCACGGTATTGTCAGTAAC+TGG	0.314467	3.1:-76204735	MS.gene049321:CDS
ACAGGTATTGTGTTGGATTC+CGG	0.317257	3.1:-76204423	MS.gene049321:intron
CTGACAATACCGTGCTCAAT+GGG	0.336851	3.1:+76204742	None:intergenic
ATCCTTCGTTTGGATCTTGC+TGG	0.344358	3.1:-76204345	MS.gene049321:CDS
TCCTAGCAGCATGAAGATTA+AGG	0.346598	3.1:-76203905	MS.gene049321:CDS
GGTGGTTCAACTATGTTTCC+TGG	0.355516	3.1:-76203967	MS.gene049321:CDS
TGTGTGCAGGCTGGATTTGC+TGG	0.360977	3.1:-76204904	MS.gene049321:intron
GAAAGTCTCAAACATGATTT+GGG	0.367813	3.1:+76204591	None:intergenic
GCTTCCATTCCGATCATTGA+TGG	0.371444	3.1:+76204078	None:intergenic
CTATTGGCCTTTGGGTTAAG+TGG	0.372320	3.1:+76204625	None:intergenic
TACTGGTGTCATGGTTGGTA+TGG	0.381549	3.1:-76204830	MS.gene049321:CDS
CCCACATGCTATCCTTCGTT+TGG	0.383523	3.1:-76204355	MS.gene049321:CDS
CGAGGTCGGCCAACAATACT+TGG	0.411313	3.1:+76204856	None:intergenic
TACGGCAACATTGTTCTTAG+TGG	0.417976	3.1:-76203988	MS.gene049321:CDS
ATGGTTGGAAAAGGACTTCT+GGG	0.420047	3.1:+76204097	None:intergenic
GGAAATTGTCCGTGACATAA+AGG	0.432407	3.1:-76204250	MS.gene049321:CDS
TATGCAAGTGGTCGTACAAC+TGG	0.432992	3.1:-76204523	MS.gene049321:intron
GTTGGCCGACCTCGTCATAC+TGG	0.436618	3.1:-76204847	MS.gene049321:CDS
CTTGTTTGTGACAATGGAAC+TGG	0.436831	3.1:-76205020	MS.gene049321:CDS
TCTTCATGCTGCTAGGAGCC+AGG	0.437433	3.1:+76203911	None:intergenic
CAACCCCTTGTTTGTGACAA+TGG	0.439095	3.1:-76205026	MS.gene049321:CDS
TTCTCTCAGTACTTTCCAGC+AGG	0.448158	3.1:-76203827	MS.gene049321:intron
GGAGCCTCAGTTAGAAGCAC+TGG	0.448567	3.1:+76204646	None:intergenic
TTGCTGGTGATGATGCTCCT+AGG	0.448710	3.1:-76204888	MS.gene049321:CDS
GCTGTGTTTCCAAGTATTGT+TGG	0.449099	3.1:-76204865	MS.gene049321:CDS
CGCCATGTATGTGGCCATCC+AGG	0.449438	3.1:-76204560	MS.gene049321:CDS
ACACTTCATGATAGAGTTGT+AGG	0.451087	3.1:+76204035	None:intergenic
GATGGACAAGTTATCACAAT+TGG	0.452391	3.1:-76204138	MS.gene049321:CDS
ATTGTGATAACTTGTCCATC+AGG	0.464957	3.1:+76204141	None:intergenic
CATTTCCTGTGGACAATGGA+TGG	0.469196	3.1:+76203670	None:intergenic
CGATCATTGATGGTTGGAAA+AGG	0.470418	3.1:+76204088	None:intergenic
CACACGCTGTACTTTCTCTC+TGG	0.472494	3.1:+76203871	None:intergenic
AGAAAGTACAGCGTGTGGAT+TGG	0.472662	3.1:-76203865	MS.gene049321:CDS
GGGATGACATGGAGAAGATC+TGG	0.479434	3.1:-76204714	MS.gene049321:CDS
GAAGCTTGCATATGTTGCCT+TGG	0.482032	3.1:-76204226	MS.gene049321:CDS
AGCCTGGATGGCCACATACA+TGG	0.486225	3.1:+76204558	None:intergenic
TCACACTGTGCCAATCTATG+AGG	0.492803	3.1:-76204388	MS.gene049321:CDS
ACTGACAATACCGTGCTCAA+TGG	0.496612	3.1:+76204741	None:intergenic
ATGAAGATCCTCACTGAGAG+AGG	0.498979	3.1:-76204300	MS.gene049321:CDS
AAGGGTGAATATGACGAGTC+TGG	0.499493	3.1:-76203694	MS.gene049321:CDS
CAGCATGAAGATTAAGGTTG+TGG	0.500086	3.1:-76203899	MS.gene049321:CDS
GTTCACCACCTCAGCTGAGC+GGG	0.502382	3.1:-76204271	MS.gene049321:CDS
AGTGCATAACCCTCATAGAT+TGG	0.503175	3.1:+76204378	None:intergenic
AAGTACAGCGTGTGGATTGG+AGG	0.503603	3.1:-76203862	MS.gene049321:CDS
CCATTCCGATCATTGATGGT+TGG	0.507915	3.1:+76204082	None:intergenic
CTCATACGGTCAGCAATACC+AGG	0.512812	3.1:+76203949	None:intergenic
TGCTGGTGATGATGCTCCTA+GGG	0.516201	3.1:-76204887	MS.gene049321:CDS
CATAGAGGGAGAGGACAGCC+TGG	0.522013	3.1:+76204542	None:intergenic
TTGGGCTTCGTCACCAACAT+AGG	0.522846	3.1:+76204795	None:intergenic
CAGTTCCATTGTCACAAACA+AGG	0.524372	3.1:+76205021	None:intergenic
GGCAACATTGTTCTTAGTGG+TGG	0.526272	3.1:-76203985	MS.gene049321:CDS
GAGCAAGGAGATCACTGCCC+TGG	0.526620	3.1:-76203929	MS.gene049321:CDS
CAGTGATCTCCTTGCTCATA+CGG	0.530919	3.1:+76203935	None:intergenic
CCAACCATCAATGATCGGAA+TGG	0.531135	3.1:-76204082	MS.gene049321:CDS
CATGGTTGGTATGGGTCAAA+AGG	0.532081	3.1:-76204821	MS.gene049321:CDS
ATTGTGTTGGATTCCGGCGA+TGG	0.537139	3.1:-76204417	MS.gene049321:CDS
ACTGGTGTCATGGTTGGTAT+GGG	0.539815	3.1:-76204829	MS.gene049321:CDS
ACCTCGTCATACTGGTGTCA+TGG	0.540702	3.1:-76204839	MS.gene049321:CDS
GACAATTTCCCGCTCAGCTG+AGG	0.541645	3.1:+76204263	None:intergenic
ATGATCGGAATGGAAGCTGC+TGG	0.546973	3.1:-76204072	MS.gene049321:CDS
ACGCTGTACTTTCTCTCTGG+TGG	0.560290	3.1:+76203874	None:intergenic
TGTACGACCACTTGCATAGA+GGG	0.561313	3.1:+76204528	None:intergenic
AGTTCCATTGTCACAAACAA+GGG	0.561709	3.1:+76205022	None:intergenic
TGTCAGTAACTGGGATGACA+TGG	0.562581	3.1:-76204725	MS.gene049321:CDS
GAGGGAGAGGACAGCCTGGA+TGG	0.565012	3.1:+76204546	None:intergenic
TTGTACGACCACTTGCATAG+AGG	0.570654	3.1:+76204527	None:intergenic
AGACTCGTCATATTCACCCT+TGG	0.570952	3.1:+76203696	None:intergenic
AATTTCCCGCTCAGCTGAGG+TGG	0.571031	3.1:+76204266	None:intergenic
AAGTTCTTGTTCATAATCCA+AGG	0.571032	3.1:+76204209	None:intergenic
ATACTTGGAAACACAGCCCT+AGG	0.573620	3.1:+76204871	None:intergenic
CGTCATACTGGTGTCATGGT+TGG	0.573856	3.1:-76204835	MS.gene049321:CDS
CTTCATGCTGCTAGGAGCCA+GGG	0.577950	3.1:+76203912	None:intergenic
GAAGCATTTCCTGTGGACAA+TGG	0.580709	3.1:+76203666	None:intergenic
CAAGCTTCTCCTTTATGTCA+CGG	0.584330	3.1:+76204241	None:intergenic
GACCACTTGCATAGAGGGAG+AGG	0.585397	3.1:+76204533	None:intergenic
CTGGCCCATCCATTGTCCAC+AGG	0.585546	3.1:-76203675	MS.gene049321:CDS
GCAAAAGATGGCAGATGCTG+AGG	0.585999	3.1:-76205054	None:intergenic
CAATGTGCCCGCCATGTATG+TGG	0.588880	3.1:-76204569	MS.gene049321:CDS
TGGATGGCCACATACATGGC+GGG	0.589918	3.1:+76204562	None:intergenic
GACGAAGCCCAATCCAAAAG+AGG	0.590961	3.1:-76204784	MS.gene049321:CDS
GATATCAGAAAAGATCTGTA+CGG	0.594697	3.1:-76204006	MS.gene049321:CDS
GTCCTCTCCCTCTATGCAAG+TGG	0.594901	3.1:-76204535	MS.gene049321:CDS
ATTTCCTGTGGACAATGGAT+GGG	0.598758	3.1:+76203671	None:intergenic
ACCTTAATCTTCATGCTGCT+AGG	0.600175	3.1:+76203904	None:intergenic
TTTGCAGATGTGGATATCCA+AGG	0.604467	3.1:-76203713	MS.gene049321:intron
CTCAAGTACCCCATTGAGCA+CGG	0.608960	3.1:-76204751	MS.gene049321:CDS
CACACTGTGCCAATCTATGA+GGG	0.612158	3.1:-76204387	MS.gene049321:CDS
TATTGCTGACCGTATGAGCA+AGG	0.626661	3.1:-76203944	MS.gene049321:CDS
GAGCCTCAGTTAGAAGCACT+GGG	0.630481	3.1:+76204647	None:intergenic
CAGAGAGAAAGTACAGCGTG+TGG	0.630640	3.1:-76203870	MS.gene049321:CDS
AGTGTGACTCACACCATCGC+CGG	0.631174	3.1:+76204404	None:intergenic
GCACGGTATTGTCAGTAACT+GGG	0.632162	3.1:-76204734	MS.gene049321:CDS
GGTCAAAAGGATGCCTATGT+TGG	0.633441	3.1:-76204808	MS.gene049321:CDS
TCCAAACGAAGGATAGCATG+TGG	0.637382	3.1:+76204354	None:intergenic
TTGTGACAATGGAACTGGAA+TGG	0.639550	3.1:-76205015	MS.gene049321:CDS
CTCTATCATGAAGTGTGATG+TGG	0.640270	3.1:-76204028	MS.gene049321:CDS
GGACTTAGAAGCATTTCCTG+TGG	0.642629	3.1:+76203659	None:intergenic
GGAAAAGGACTTCTGGGCAA+CGG	0.648496	3.1:+76204103	None:intergenic
GGAAAGTACTGAGAGAAGCA+AGG	0.656348	3.1:+76203833	None:intergenic
GTTCCATTGTCACAAACAAG+GGG	0.658903	3.1:+76205023	None:intergenic
CAATGGAACTGGAATGGTGA+AGG	0.661223	3.1:-76205009	MS.gene049321:intron
TGAAGATCCTCACTGAGAGA+GGG	0.661425	3.1:-76204299	MS.gene049321:CDS
TTATCACAATTGGAGCTGAG+AGG	0.664936	3.1:-76204128	MS.gene049321:CDS
TGAGGCTCCACTTAACCCAA+AGG	0.668440	3.1:-76204632	MS.gene049321:CDS
TGACACCAGTATGACGAGGT+CGG	0.672228	3.1:+76204842	None:intergenic
GCACCCAGTGCTTCTAACTG+AGG	0.673440	3.1:-76204650	MS.gene049321:CDS
CCAAACGAAGGATAGCATGT+GGG	0.675471	3.1:+76204355	None:intergenic
TTGCAGATGTGGATATCCAA+GGG	0.681771	3.1:-76203712	MS.gene049321:intron
CTGGATGGCCACATACATGG+CGG	0.687535	3.1:+76204561	None:intergenic
CTTCATGATAGAGTTGTAGG+TGG	0.694993	3.1:+76204038	None:intergenic
GACCAGCAAGATCCAAACGA+AGG	0.704830	3.1:+76204343	None:intergenic
ACATGTACCCTCTCTCAGTG+AGG	0.709732	3.1:+76204292	None:intergenic
TGACAATACCGTGCTCAATG+GGG	0.720666	3.1:+76204743	None:intergenic
TGTTCACCACCTCAGCTGAG+CGG	0.722178	3.1:-76204272	MS.gene049321:CDS
ACCATGACACCAGTATGACG+AGG	0.732971	3.1:+76204838	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTCAAAAATGAAAAGATTGA+GGG	+	chr3.1:76204267-76204286	None:intergenic	20.0%
!!	TTTCAAAAATGAAAAGATTG+AGG	+	chr3.1:76204268-76204287	None:intergenic	20.0%
!!!	AATCTTTTCATTTTTGAAAC+AGG	-	chr3.1:76204269-76204288	MS.gene049321:CDS	20.0%
	AAAAACTATGAACTTCCTGA+TGG	-	chr3.1:76204554-76204573	MS.gene049321:CDS	30.0%
	AAGTTCTTGTTCATAATCCA+AGG	+	chr3.1:76204504-76204523	None:intergenic	30.0%
	GAAAGTCTCAAACATGATTT+GGG	+	chr3.1:76204122-76204141	None:intergenic	30.0%
	GACATCACATACATTATACA+TGG	+	chr3.1:76204940-76204959	None:intergenic	30.0%
	GATATCAGAAAAGATCTGTA+CGG	-	chr3.1:76204704-76204723	MS.gene049321:CDS	30.0%
	TGAAAGTCTCAAACATGATT+TGG	+	chr3.1:76204123-76204142	None:intergenic	30.0%
!	AAGAATACCTCTTTTGGATT+GGG	+	chr3.1:76203936-76203955	None:intergenic	30.0%
!	TAAGAATACCTCTTTTGGAT+TGG	+	chr3.1:76203937-76203956	None:intergenic	30.0%
!!!	TTGTTTTTGTTTTGCAGATG+TGG	-	chr3.1:76204987-76205006	MS.gene049321:intron	30.0%
!!!	TTTTGAAACAGGTATTGTGT+TGG	-	chr3.1:76204280-76204299	MS.gene049321:CDS	30.0%
	AAGATTGAGGGACAGAATAA+TGG	+	chr3.1:76204255-76204274	None:intergenic	35.0%
	ACACTTCATGATAGAGTTGT+AGG	+	chr3.1:76204678-76204697	None:intergenic	35.0%
	AGTTCCATTGTCACAAACAA+GGG	+	chr3.1:76203691-76203710	None:intergenic	35.0%
	GATGGACAAGTTATCACAAT+TGG	-	chr3.1:76204572-76204591	MS.gene049321:CDS	35.0%
	GTGATAAAATAATCACCTGC+TGG	+	chr3.1:76204901-76204920	None:intergenic	35.0%
	TTCATCTACAATTTGTGTGC+AGG	-	chr3.1:76203793-76203812	MS.gene049321:intron	35.0%
!	ATTGTGATAACTTGTCCATC+AGG	+	chr3.1:76204572-76204591	None:intergenic	35.0%
!	CTTTTCCAACCATCAATGAT+CGG	-	chr3.1:76204623-76204642	MS.gene049321:CDS	35.0%
!	GAGAGTAAGAATACCTCTTT+TGG	+	chr3.1:76203942-76203961	None:intergenic	35.0%
!	TTGGGTCATCTTTTCTCTAT+TGG	+	chr3.1:76204104-76204123	None:intergenic	35.0%
!!!	ATCTTTTCTCTATTGGCCTT+TGG	+	chr3.1:76204097-76204116	None:intergenic	35.0%
!!!	TCTTTTCTCTATTGGCCTTT+GGG	+	chr3.1:76204096-76204115	None:intergenic	35.0%
	ACCTTAATCTTCATGCTGCT+AGG	+	chr3.1:76204809-76204828	None:intergenic	40.0%
	AGTGCATAACCCTCATAGAT+TGG	+	chr3.1:76204335-76204354	None:intergenic	40.0%
	ATGGTTGGAAAAGGACTTCT+GGG	+	chr3.1:76204616-76204635	None:intergenic	40.0%
	ATTTCCTGTGGACAATGGAT+GGG	+	chr3.1:76205042-76205061	None:intergenic	40.0%
	CAAGCTTCTCCTTTATGTCA+CGG	+	chr3.1:76204472-76204491	None:intergenic	40.0%
	CAGCATGAAGATTAAGGTTG+TGG	-	chr3.1:76204811-76204830	MS.gene049321:CDS	40.0%
	CAGTTCCATTGTCACAAACA+AGG	+	chr3.1:76203692-76203711	None:intergenic	40.0%
	CTCTATCATGAAGTGTGATG+TGG	-	chr3.1:76204682-76204701	MS.gene049321:CDS	40.0%
	CTTCATGATAGAGTTGTAGG+TGG	+	chr3.1:76204675-76204694	None:intergenic	40.0%
	CTTGTTTGTGACAATGGAAC+TGG	-	chr3.1:76203690-76203709	MS.gene049321:CDS	40.0%
	GGAAATTGTCCGTGACATAA+AGG	-	chr3.1:76204460-76204479	MS.gene049321:intron	40.0%
	GTTCCATTGTCACAAACAAG+GGG	+	chr3.1:76203690-76203709	None:intergenic	40.0%
	TACGGCAACATTGTTCTTAG+TGG	-	chr3.1:76204722-76204741	MS.gene049321:CDS	40.0%
	TCCTAGCAGCATGAAGATTA+AGG	-	chr3.1:76204805-76204824	MS.gene049321:CDS	40.0%
	TTATCACAATTGGAGCTGAG+AGG	-	chr3.1:76204582-76204601	MS.gene049321:CDS	40.0%
	TTGCAGATGTGGATATCCAA+GGG	-	chr3.1:76204998-76205017	MS.gene049321:intron	40.0%
	TTGTGACAATGGAACTGGAA+TGG	-	chr3.1:76203695-76203714	MS.gene049321:CDS	40.0%
	TTTGCAGATGTGGATATCCA+AGG	-	chr3.1:76204997-76205016	MS.gene049321:intron	40.0%
!	ACAGGTATTGTGTTGGATTC+CGG	-	chr3.1:76204287-76204306	MS.gene049321:CDS	40.0%
!	CGATCATTGATGGTTGGAAA+AGG	+	chr3.1:76204625-76204644	None:intergenic	40.0%
!	GCTGTGTTTCCAAGTATTGT+TGG	-	chr3.1:76203845-76203864	MS.gene049321:CDS	40.0%
	AAGGGTGAATATGACGAGTC+TGG	-	chr3.1:76205016-76205035	MS.gene049321:CDS	45.0%
	ACTGACAATACCGTGCTCAA+TGG	+	chr3.1:76203972-76203991	None:intergenic	45.0%
	AGACTCGTCATATTCACCCT+TGG	+	chr3.1:76205017-76205036	None:intergenic	45.0%
	AGCACGGTATTGTCAGTAAC+TGG	-	chr3.1:76203975-76203994	MS.gene049321:CDS	45.0%
	ATACTTGGAAACACAGCCCT+AGG	+	chr3.1:76203842-76203861	None:intergenic	45.0%
	ATCCTTCGTTTGGATCTTGC+TGG	-	chr3.1:76204365-76204384	MS.gene049321:CDS	45.0%
	ATGAAGATCCTCACTGAGAG+AGG	-	chr3.1:76204410-76204429	MS.gene049321:CDS	45.0%
	CAATGGAACTGGAATGGTGA+AGG	-	chr3.1:76203701-76203720	MS.gene049321:CDS	45.0%
	CACACTGTGCCAATCTATGA+GGG	-	chr3.1:76204323-76204342	MS.gene049321:CDS	45.0%
	CAGTGATCTCCTTGCTCATA+CGG	+	chr3.1:76204778-76204797	None:intergenic	45.0%
	CATTTCCTGTGGACAATGGA+TGG	+	chr3.1:76205043-76205062	None:intergenic	45.0%
	CCAAACGAAGGATAGCATGT+GGG	+	chr3.1:76204358-76204377	None:intergenic	45.0%
	CCAACCATCAATGATCGGAA+TGG	-	chr3.1:76204628-76204647	MS.gene049321:CDS	45.0%
	CTGACAATACCGTGCTCAAT+GGG	+	chr3.1:76203971-76203990	None:intergenic	45.0%
	GAAGCTTGCATATGTTGCCT+TGG	-	chr3.1:76204484-76204503	MS.gene049321:intron	45.0%
	GATGGTTGGAAAAGGACTTC+TGG	+	chr3.1:76204617-76204636	None:intergenic	45.0%
	GCACGGTATTGTCAGTAACT+GGG	-	chr3.1:76203976-76203995	MS.gene049321:CDS	45.0%
	GCTTCCATTCCGATCATTGA+TGG	+	chr3.1:76204635-76204654	None:intergenic	45.0%
	GGCAACATTGTTCTTAGTGG+TGG	-	chr3.1:76204725-76204744	MS.gene049321:CDS	45.0%
	GGTCAAAAGGATGCCTATGT+TGG	-	chr3.1:76203902-76203921	MS.gene049321:CDS	45.0%
	GGTGGTTCAACTATGTTTCC+TGG	-	chr3.1:76204743-76204762	MS.gene049321:CDS	45.0%
	TATGCAAGTGGTCGTACAAC+TGG	-	chr3.1:76204187-76204206	MS.gene049321:CDS	45.0%
	TATTGCTGACCGTATGAGCA+AGG	-	chr3.1:76204766-76204785	MS.gene049321:CDS	45.0%
	TCACACTGTGCCAATCTATG+AGG	-	chr3.1:76204322-76204341	MS.gene049321:CDS	45.0%
	TCCAAACGAAGGATAGCATG+TGG	+	chr3.1:76204359-76204378	None:intergenic	45.0%
	TCTACAATTTGTGTGCAGGC+TGG	-	chr3.1:76203797-76203816	MS.gene049321:intron	45.0%
	TGAAGATCCTCACTGAGAGA+GGG	-	chr3.1:76204411-76204430	MS.gene049321:CDS	45.0%
	TGACAATACCGTGCTCAATG+GGG	+	chr3.1:76203970-76203989	None:intergenic	45.0%
	TGTACGACCACTTGCATAGA+GGG	+	chr3.1:76204185-76204204	None:intergenic	45.0%
	TGTCAGTAACTGGGATGACA+TGG	-	chr3.1:76203985-76204004	MS.gene049321:CDS	45.0%
	TTCTCTCAGTACTTTCCAGC+AGG	-	chr3.1:76204883-76204902	MS.gene049321:CDS	45.0%
	TTGTACGACCACTTGCATAG+AGG	+	chr3.1:76204186-76204205	None:intergenic	45.0%
!	AGAAAGTACAGCGTGTGGAT+TGG	-	chr3.1:76204845-76204864	MS.gene049321:CDS	45.0%
!	CAACCCCTTGTTTGTGACAA+TGG	-	chr3.1:76203684-76203703	MS.gene049321:CDS	45.0%
!	CATGGTTGGTATGGGTCAAA+AGG	-	chr3.1:76203889-76203908	MS.gene049321:CDS	45.0%
!	CCATTCCGATCATTGATGGT+TGG	+	chr3.1:76204631-76204650	None:intergenic	45.0%
!	GAAGCATTTCCTGTGGACAA+TGG	+	chr3.1:76205047-76205066	None:intergenic	45.0%
!!	ACTGGTGTCATGGTTGGTAT+GGG	-	chr3.1:76203881-76203900	MS.gene049321:CDS	45.0%
!!	CTATTGGCCTTTGGGTTAAG+TGG	+	chr3.1:76204088-76204107	None:intergenic	45.0%
!!	GGAAAGTACTGAGAGAAGCA+AGG	+	chr3.1:76204880-76204899	None:intergenic	45.0%
!!	TACTGGTGTCATGGTTGGTA+TGG	-	chr3.1:76203880-76203899	MS.gene049321:CDS	45.0%
	ACATGTACCCTCTCTCAGTG+AGG	+	chr3.1:76204421-76204440	None:intergenic	50.0%
	ACCATGACACCAGTATGACG+AGG	+	chr3.1:76203875-76203894	None:intergenic	50.0%
	ACGCTGTACTTTCTCTCTGG+TGG	+	chr3.1:76204839-76204858	None:intergenic	50.0%
	ATGATCGGAATGGAAGCTGC+TGG	-	chr3.1:76204638-76204657	MS.gene049321:CDS	50.0%
	ATTGTGTTGGATTCCGGCGA+TGG	-	chr3.1:76204293-76204312	MS.gene049321:CDS	50.0%
	CACACGCTGTACTTTCTCTC+TGG	+	chr3.1:76204842-76204861	None:intergenic	50.0%
	CCCACATGCTATCCTTCGTT+TGG	-	chr3.1:76204355-76204374	MS.gene049321:CDS	50.0%
	CTCAAGTACCCCATTGAGCA+CGG	-	chr3.1:76203959-76203978	MS.gene049321:CDS	50.0%
	CTCATACGGTCAGCAATACC+AGG	+	chr3.1:76204764-76204783	None:intergenic	50.0%
	GACCAGCAAGATCCAAACGA+AGG	+	chr3.1:76204370-76204389	None:intergenic	50.0%
	GACGAAGCCCAATCCAAAAG+AGG	-	chr3.1:76203926-76203945	MS.gene049321:CDS	50.0%
	GGAAAAGGACTTCTGGGCAA+CGG	+	chr3.1:76204610-76204629	None:intergenic	50.0%
	GGGATGACATGGAGAAGATC+TGG	-	chr3.1:76203996-76204015	MS.gene049321:CDS	50.0%
	TGACACCAGTATGACGAGGT+CGG	+	chr3.1:76203871-76203890	None:intergenic	50.0%
	TGAGGCTCCACTTAACCCAA+AGG	-	chr3.1:76204078-76204097	MS.gene049321:CDS	50.0%
	TTGGGCTTCGTCACCAACAT+AGG	+	chr3.1:76203918-76203937	None:intergenic	50.0%
!	ACCTCGTCATACTGGTGTCA+TGG	-	chr3.1:76203871-76203890	MS.gene049321:CDS	50.0%
!	CAGAGAGAAAGTACAGCGTG+TGG	-	chr3.1:76204840-76204859	MS.gene049321:CDS	50.0%
!	TGCTGGTGATGATGCTCCTA+GGG	-	chr3.1:76203823-76203842	MS.gene049321:intron	50.0%
!	TTGCTGGTGATGATGCTCCT+AGG	-	chr3.1:76203822-76203841	MS.gene049321:intron	50.0%
!!	AAGTACAGCGTGTGGATTGG+AGG	-	chr3.1:76204848-76204867	MS.gene049321:CDS	50.0%
!!	CGTCATACTGGTGTCATGGT+TGG	-	chr3.1:76203875-76203894	MS.gene049321:CDS	50.0%
!!	GAGCCTCAGTTAGAAGCACT+GGG	+	chr3.1:76204066-76204085	None:intergenic	50.0%
	AATTTCCCGCTCAGCTGAGG+TGG	+	chr3.1:76204447-76204466	None:intergenic	55.0%
	AGCCTGGATGGCCACATACA+TGG	+	chr3.1:76204155-76204174	None:intergenic	55.0%
	AGTGTGACTCACACCATCGC+CGG	+	chr3.1:76204309-76204328	None:intergenic	55.0%
	CAATGTGCCCGCCATGTATG+TGG	-	chr3.1:76204141-76204160	MS.gene049321:CDS	55.0%
	CGAGGTCGGCCAACAATACT+TGG	+	chr3.1:76203857-76203876	None:intergenic	55.0%
	CTGGATGGCCACATACATGG+CGG	+	chr3.1:76204152-76204171	None:intergenic	55.0%
	CTTCATGCTGCTAGGAGCCA+GGG	+	chr3.1:76204801-76204820	None:intergenic	55.0%
	GACAATTTCCCGCTCAGCTG+AGG	+	chr3.1:76204450-76204469	None:intergenic	55.0%
	GACCACTTGCATAGAGGGAG+AGG	+	chr3.1:76204180-76204199	None:intergenic	55.0%
	GCACCCAGTGCTTCTAACTG+AGG	-	chr3.1:76204060-76204079	MS.gene049321:CDS	55.0%
	GTCCTCTCCCTCTATGCAAG+TGG	-	chr3.1:76204175-76204194	MS.gene049321:CDS	55.0%
	TCTTCATGCTGCTAGGAGCC+AGG	+	chr3.1:76204802-76204821	None:intergenic	55.0%
	TGGATGGCCACATACATGGC+GGG	+	chr3.1:76204151-76204170	None:intergenic	55.0%
	TGTGTGCAGGCTGGATTTGC+TGG	-	chr3.1:76203806-76203825	MS.gene049321:intron	55.0%
	TGTTCACCACCTCAGCTGAG+CGG	-	chr3.1:76204438-76204457	MS.gene049321:intron	55.0%
!	GGAGCCTCAGTTAGAAGCAC+TGG	+	chr3.1:76204067-76204086	None:intergenic	55.0%
!!	AGAAGCACTGGGTGCTCTTC+AGG	+	chr3.1:76204055-76204074	None:intergenic	55.0%
	CATAGAGGGAGAGGACAGCC+TGG	+	chr3.1:76204171-76204190	None:intergenic	60.0%
	CGCCATGTATGTGGCCATCC+AGG	-	chr3.1:76204150-76204169	MS.gene049321:CDS	60.0%
	CTGGCCCATCCATTGTCCAC+AGG	-	chr3.1:76205035-76205054	MS.gene049321:CDS	60.0%
	GAGCAAGGAGATCACTGCCC+TGG	-	chr3.1:76204781-76204800	MS.gene049321:CDS	60.0%
	GTTCACCACCTCAGCTGAGC+GGG	-	chr3.1:76204439-76204458	MS.gene049321:intron	60.0%
	GTTGGCCGACCTCGTCATAC+TGG	-	chr3.1:76203863-76203882	MS.gene049321:CDS	60.0%
	GAGGGAGAGGACAGCCTGGA+TGG	+	chr3.1:76204167-76204186	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.1	gene	76203663	76205069	76203663	ID=MS.gene049321
chr3.1	mRNA	76203663	76205069	76203663	ID=MS.gene049321.t1;Parent=MS.gene049321
chr3.1	exon	76205010	76205069	76205010	ID=MS.gene049321.t1.exon1;Parent=MS.gene049321.t1
chr3.1	CDS	76205010	76205069	76205010	ID=cds.MS.gene049321.t1;Parent=MS.gene049321.t1
chr3.1	exon	76204524	76204917	76204524	ID=MS.gene049321.t1.exon2;Parent=MS.gene049321.t1
chr3.1	CDS	76204524	76204917	76204524	ID=cds.MS.gene049321.t1;Parent=MS.gene049321.t1
chr3.1	exon	76203828	76204441	76203828	ID=MS.gene049321.t1.exon3;Parent=MS.gene049321.t1
chr3.1	CDS	76203828	76204441	76203828	ID=cds.MS.gene049321.t1;Parent=MS.gene049321.t1
chr3.1	exon	76203663	76203728	76203663	ID=MS.gene049321.t1.exon4;Parent=MS.gene049321.t1
chr3.1	CDS	76203663	76203728	76203663	ID=cds.MS.gene049321.t1;Parent=MS.gene049321.t1

Gene Sequence

>MS.gene049321

ATGGCAGATGCTGAGGATATTCAACCCCTTGTTTGTGACAATGGAACTGGAATGGTGAAGGTTAGTAATTCAAAAAAAAAATCTGTCATTTTTGTGTGTTTGATTTTTGTTTTTGTTACTGAACGATGTGTTCATCTACAATTTGTGTGCAGGCTGGATTTGCTGGTGATGATGCTCCTAGGGCTGTGTTTCCAAGTATTGTTGGCCGACCTCGTCATACTGGTGTCATGGTTGGTATGGGTCAAAAGGATGCCTATGTTGGTGACGAAGCCCAATCCAAAAGAGGTATTCTTACTCTCAAGTACCCCATTGAGCACGGTATTGTCAGTAACTGGGATGACATGGAGAAGATCTGGCATCACACATTTTACAATGAATTGCGTGTTGCTCCTGAAGAGCACCCAGTGCTTCTAACTGAGGCTCCACTTAACCCAAAGGCCAATAGAGAAAAGATGACCCAAATCATGTTTGAGACTTTCAATGTGCCCGCCATGTATGTGGCCATCCAGGCTGTCCTCTCCCTCTATGCAAGTGGTCGTACAACTGGTTCGTATTTTGACTATCTTTTTGAGAACATACTTTTGTCATTCCATTATTCTGTCCCTCAATCTTTTCATTTTTGAAACAGGTATTGTGTTGGATTCCGGCGATGGTGTGAGTCACACTGTGCCAATCTATGAGGGTTATGCACTCCCACATGCTATCCTTCGTTTGGATCTTGCTGGTCGTGATCTAACTGATTCTTTGATGAAGATCCTCACTGAGAGAGGGTACATGTTCACCACCTCAGCTGAGCGGGAAATTGTCCGTGACATAAAGGAGAAGCTTGCATATGTTGCCTTGGATTATGAACAAGAACTTGAGACTGCAAAGAGCAGTTCTTCAATTGAGAAAAACTATGAACTTCCTGATGGACAAGTTATCACAATTGGAGCTGAGAGGTTCCGTTGCCCAGAAGTCCTTTTCCAACCATCAATGATCGGAATGGAAGCTGCTGGAATTCACGAGACCACCTACAACTCTATCATGAAGTGTGATGTGGATATCAGAAAAGATCTGTACGGCAACATTGTTCTTAGTGGTGGTTCAACTATGTTTCCTGGTATTGCTGACCGTATGAGCAAGGAGATCACTGCCCTGGCTCCTAGCAGCATGAAGATTAAGGTTGTGGCTCCACCAGAGAGAAAGTACAGCGTGTGGATTGGAGGATCAATCCTTGCTTCTCTCAGTACTTTCCAGCAGGTGATTATTTTATCACAGATTAGCACATTTTGCCATGTATAATGTATGTGATGTCAATTTTATCTAATTCTACAATTTATTTTTGTTTTTGTTTTGCAGATGTGGATATCCAAGGGTGAATATGACGAGTCTGGCCCATCCATTGTCCACAGGAAATGCTTCTAA

Protein sequence

>MS.gene049321.t1

MADAEDIQPLVCDNGTGMVKAGFAGDDAPRAVFPSIVGRPRHTGVMVGMGQKDAYVGDEAQSKRGILTLKYPIEHGIVSNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRLDLAGRDLTDSLMKILTERGYMFTTSAEREIVRDIKEKLAYVALDYEQELETAKSSSSIEKNYELPDGQVITIGAERFRCPEVLFQPSMIGMEAAGIHETTYNSIMKCDVDIRKDLYGNIVLSGGSTMFPGIADRMSKEITALAPSSMKIKVVAPPERKYSVWIGGSILASLSTFQQMWISKGEYDESGPSIVHRKCF