Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97789.t1 | XP_013447780.1 | 92.1 | 252 | 20 | 0 | 1 | 252 | 17 | 268 | 2.10E-115 | 425.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97789.t1 | P93332 | 90.9 | 252 | 23 | 0 | 1 | 252 | 17 | 268 | 3.5e-116 | 419.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97789.t1 | A0A072TX72 | 92.1 | 252 | 20 | 0 | 1 | 252 | 17 | 268 | 1.5e-115 | 425.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05240 | MS.gene97789 | 0.800467 | 1.42E-48 | -1.69E-46 |
| MS.gene054742 | MS.gene97789 | 0.834061 | 3.73E-56 | -1.69E-46 |
| MS.gene056920 | MS.gene97789 | 0.828427 | 9.05E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 59 sgRNAs with CRISPR-Local
Find 73 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATTTCGTTCTTGGTGTTCT+TGG | 0.169280 | 7.3:-82113150 | MS.gene97789:CDS |
| CACACATCCAAATGAGTTTA+TGG | 0.188728 | 7.3:+82112898 | None:intergenic |
| CCACTTCGTGTCCAAGTTCT+TGG | 0.244317 | 7.3:-82112622 | MS.gene97789:CDS |
| TCGATTTCTTCTTGTATATA+CGG | 0.244759 | 7.3:+82113020 | None:intergenic |
| CTCATTACCATAAACTCATT+TGG | 0.319248 | 7.3:-82112905 | MS.gene97789:CDS |
| AGGTAACGTCATTTCGTTCT+TGG | 0.323739 | 7.3:-82113159 | MS.gene97789:CDS |
| CTTAACTTTCAAGTTACTTT+CGG | 0.328483 | 7.3:-82112704 | MS.gene97789:CDS |
| AATGTGTTGGGTTTCGTATT+GGG | 0.328786 | 7.3:-82112068 | MS.gene97789:CDS |
| CTTTGTTCAGCTCCATGCTT+TGG | 0.359151 | 7.3:-82112964 | MS.gene97789:CDS |
| AATACGAAACCCAACACATT+TGG | 0.368529 | 7.3:+82112071 | None:intergenic |
| AAATGTGTTGGGTTTCGTAT+TGG | 0.380441 | 7.3:-82112069 | MS.gene97789:CDS |
| CACAAACCCATCCAAGAACT+TGG | 0.408159 | 7.3:+82112611 | None:intergenic |
| ATTTAAACTCCAGAGCCAAT+AGG | 0.432197 | 7.3:+82111864 | None:intergenic |
| TTCGTGTCCAAGTTCTTGGA+TGG | 0.434485 | 7.3:-82112618 | MS.gene97789:CDS |
| ATACGAAACCCAACACATTT+GGG | 0.439248 | 7.3:+82112072 | None:intergenic |
| TGCTGCTGTATGCAATCTAT+AGG | 0.452278 | 7.3:-82112034 | MS.gene97789:CDS |
| AATAATACCCTTGCATCTCT+TGG | 0.455895 | 7.3:+82112836 | None:intergenic |
| GTTCAAAGTGAGGAACCTAT+TGG | 0.474459 | 7.3:-82111879 | MS.gene97789:CDS |
| AGTGAGGAACCTATTGGCTC+TGG | 0.490260 | 7.3:-82111873 | MS.gene97789:CDS |
| GTACATACCACAATGCTTAG+TGG | 0.491986 | 7.3:+82112559 | None:intergenic |
| GCCACAATGTGGTTTGGCTA+CGG | 0.514248 | 7.3:-82112371 | MS.gene97789:CDS |
| TTGAGTGCCACAATGTGGTT+TGG | 0.514750 | 7.3:-82112377 | MS.gene97789:CDS |
| TCGTGTCCAAGTTCTTGGAT+GGG | 0.519498 | 7.3:-82112617 | MS.gene97789:CDS |
| CTGAACAAAGCTACTAGGTA+AGG | 0.520832 | 7.3:+82112977 | None:intergenic |
| TGGAGCTGAACAAAGCTACT+AGG | 0.521833 | 7.3:+82112972 | None:intergenic |
| CTGCAAAGACACTAACACTA+AGG | 0.526938 | 7.3:+82112584 | None:intergenic |
| GTGCAGCTCCCAAATGTGTT+GGG | 0.530668 | 7.3:-82112080 | MS.gene97789:intron |
| GAACAAGGGTGATGATAATG+AGG | 0.540496 | 7.3:-82111907 | MS.gene97789:CDS |
| ATGCAATCTATAGGAATGGT+GGG | 0.547366 | 7.3:-82112025 | MS.gene97789:CDS |
| CAAATGAGTTTATGGTAATG+AGG | 0.551806 | 7.3:+82112906 | None:intergenic |
| CATAAACTCATTTGGATGTG+TGG | 0.553344 | 7.3:-82112897 | MS.gene97789:CDS |
| GTTACTTTCGGCAATGAACG+TGG | 0.564481 | 7.3:-82112692 | MS.gene97789:CDS |
| TACAAGAAGAAATCGACAGA+GGG | 0.564739 | 7.3:-82113013 | MS.gene97789:CDS |
| GACACGAAGTGGACCATGCA+CGG | 0.579340 | 7.3:+82112633 | None:intergenic |
| TGGCTACGGTCTCTTCCTCA+AGG | 0.581206 | 7.3:-82112357 | MS.gene97789:CDS |
| TGTGCAGCTCCCAAATGTGT+TGG | 0.581637 | 7.3:-82112081 | MS.gene97789:intron |
| TATTGTTATAGAGACCCAAT+CGG | 0.581864 | 7.3:-82111952 | MS.gene97789:CDS |
| GATGTACAATATGATGTAGA+TGG | 0.583280 | 7.3:+82112868 | None:intergenic |
| GTCACTAACTATGCCGTGCA+TGG | 0.586420 | 7.3:-82112646 | MS.gene97789:CDS |
| CCAAGAACTTGGACACGAAG+TGG | 0.588617 | 7.3:+82112622 | None:intergenic |
| CGGCATAGTTAGTGACTATG+AGG | 0.591246 | 7.3:+82112653 | None:intergenic |
| GACTATGAGGATCAAAGCAA+AGG | 0.596059 | 7.3:+82112666 | None:intergenic |
| CTGTATGCAATCTATAGGAA+TGG | 0.607405 | 7.3:-82112029 | MS.gene97789:CDS |
| CTATGCACCAAGAGATGCAA+GGG | 0.607841 | 7.3:-82112843 | MS.gene97789:intron |
| TGCATAGTACAACCAAAGCA+TGG | 0.611574 | 7.3:+82112952 | None:intergenic |
| TATGCAATCTATAGGAATGG+TGG | 0.615800 | 7.3:-82112026 | MS.gene97789:CDS |
| CAGCAATGCATATGTCCTTG+AGG | 0.616655 | 7.3:+82112342 | None:intergenic |
| AGAGTGAATGACAAATTGAA+AGG | 0.622293 | 7.3:+82112404 | None:intergenic |
| TACTTTCGGCAATGAACGTG+GGG | 0.627256 | 7.3:-82112690 | MS.gene97789:CDS |
| TTACTTTCGGCAATGAACGT+GGG | 0.632501 | 7.3:-82112691 | MS.gene97789:CDS |
| TGCAAAGACACTAACACTAA+GGG | 0.634955 | 7.3:+82112585 | None:intergenic |
| TGCAATCTATAGGAATGGTG+GGG | 0.637303 | 7.3:-82112024 | MS.gene97789:CDS |
| TGCAGCACCACTAAGCATTG+TGG | 0.637500 | 7.3:-82112566 | MS.gene97789:intron |
| TGAACAAGAAAAGAAGAACA+AGG | 0.638374 | 7.3:-82111922 | MS.gene97789:CDS |
| GAACAAGAAAAGAAGAACAA+GGG | 0.658330 | 7.3:-82111921 | MS.gene97789:CDS |
| TCTATGCACCAAGAGATGCA+AGG | 0.675820 | 7.3:-82112844 | MS.gene97789:CDS |
| TCACATTGAGTGCCACAATG+TGG | 0.691357 | 7.3:-82112382 | MS.gene97789:CDS |
| ATACAAGAAGAAATCGACAG+AGG | 0.715649 | 7.3:-82113014 | MS.gene97789:CDS |
| ACCGTAGCCAAACCACATTG+TGG | 0.726020 | 7.3:+82112370 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAGAAAAAATAAAATTAG+TGG | - | chr7.3:82112725-82112744 | MS.gene97789:intron | 10.0% |
| !! | ATTTAAATAAATACTTACAT+TGG | + | chr7.3:82111901-82111920 | None:intergenic | 10.0% |
| !! | CATTCAATAATAAATATTTA+AGG | + | chr7.3:82112294-82112313 | None:intergenic | 10.0% |
| !! | TTATATATATTAATTGAGTA+AGG | + | chr7.3:82112501-82112520 | None:intergenic | 10.0% |
| !! | AGATATTAATGTTCAATTAT+TGG | + | chr7.3:82112242-82112261 | None:intergenic | 15.0% |
| !! | GATTTCATCAAAAAATAATT+AGG | + | chr7.3:82112850-82112869 | None:intergenic | 15.0% |
| !!! | TTTTCTTGAAATTTATATGT+AGG | - | chr7.3:82112561-82112580 | MS.gene97789:intron | 15.0% |
| !! | AAAACAATCAAATTATGACA+AGG | + | chr7.3:82112774-82112793 | None:intergenic | 20.0% |
| !! | TTAATGTTCAATTATTGGAT+TGG | + | chr7.3:82112237-82112256 | None:intergenic | 20.0% |
| !!! | AGTTTGATTTATGAACTTAA+TGG | - | chr7.3:82112529-82112548 | MS.gene97789:intron | 20.0% |
| ! | TCGATTTCTTCTTGTATATA+CGG | + | chr7.3:82112006-82112025 | None:intergenic | 25.0% |
| !! | TCTATAACAATACTTTTGAG+TGG | + | chr7.3:82113063-82113082 | None:intergenic | 25.0% |
| !!! | CTTAACTTTCAAGTTACTTT+CGG | - | chr7.3:82112319-82112338 | MS.gene97789:intron | 25.0% |
| !!! | TTGTTCAATTTTTTCCGATT+GGG | + | chr7.3:82113088-82113107 | None:intergenic | 25.0% |
| AGAGTGAATGACAAATTGAA+AGG | + | chr7.3:82112622-82112641 | None:intergenic | 30.0% | |
| CTCATTACCATAAACTCATT+TGG | - | chr7.3:82112118-82112137 | MS.gene97789:intron | 30.0% | |
| GAACAAGAAAAGAAGAACAA+GGG | - | chr7.3:82113102-82113121 | MS.gene97789:intron | 30.0% | |
| GATGTACAATATGATGTAGA+TGG | + | chr7.3:82112158-82112177 | None:intergenic | 30.0% | |
| TATTGTTATAGAGACCCAAT+CGG | - | chr7.3:82113071-82113090 | MS.gene97789:intron | 30.0% | |
| TGAACAAGAAAAGAAGAACA+AGG | - | chr7.3:82113101-82113120 | MS.gene97789:intron | 30.0% | |
| TGGGGAAAAAACTATGAAAA+AGG | - | chr7.3:82113017-82113036 | MS.gene97789:CDS | 30.0% | |
| ! | CAAATGAGTTTATGGTAATG+AGG | + | chr7.3:82112120-82112139 | None:intergenic | 30.0% |
| !!! | CTTGTTCAATTTTTTCCGAT+TGG | + | chr7.3:82113089-82113108 | None:intergenic | 30.0% |
| AAATGTGTTGGGTTTCGTAT+TGG | - | chr7.3:82112954-82112973 | MS.gene97789:CDS | 35.0% | |
| AATAATACCCTTGCATCTCT+TGG | + | chr7.3:82112190-82112209 | None:intergenic | 35.0% | |
| AATACGAAACCCAACACATT+TGG | + | chr7.3:82112955-82112974 | None:intergenic | 35.0% | |
| AATGTGTTGGGTTTCGTATT+GGG | - | chr7.3:82112955-82112974 | MS.gene97789:CDS | 35.0% | |
| ATACAAGAAGAAATCGACAG+AGG | - | chr7.3:82112009-82112028 | MS.gene97789:CDS | 35.0% | |
| ATACGAAACCCAACACATTT+GGG | + | chr7.3:82112954-82112973 | None:intergenic | 35.0% | |
| ATGCAATCTATAGGAATGGT+GGG | - | chr7.3:82112998-82113017 | MS.gene97789:CDS | 35.0% | |
| CACACATCCAAATGAGTTTA+TGG | + | chr7.3:82112128-82112147 | None:intergenic | 35.0% | |
| CATAAACTCATTTGGATGTG+TGG | - | chr7.3:82112126-82112145 | MS.gene97789:intron | 35.0% | |
| CTGTATGCAATCTATAGGAA+TGG | - | chr7.3:82112994-82113013 | MS.gene97789:CDS | 35.0% | |
| TACAAGAAGAAATCGACAGA+GGG | - | chr7.3:82112010-82112029 | MS.gene97789:CDS | 35.0% | |
| TATGCAATCTATAGGAATGG+TGG | - | chr7.3:82112997-82113016 | MS.gene97789:CDS | 35.0% | |
| TGAGGAAAAAGTTCAAAGTG+AGG | - | chr7.3:82113134-82113153 | MS.gene97789:intron | 35.0% | |
| TGCAAAGACACTAACACTAA+GGG | + | chr7.3:82112441-82112460 | None:intergenic | 35.0% | |
| !! | ATACTTTTGAGTGGCTCAAT+TGG | + | chr7.3:82113054-82113073 | None:intergenic | 35.0% |
| CTGAACAAAGCTACTAGGTA+AGG | + | chr7.3:82112049-82112068 | None:intergenic | 40.0% | |
| CTGCAAAGACACTAACACTA+AGG | + | chr7.3:82112442-82112461 | None:intergenic | 40.0% | |
| GACTATGAGGATCAAAGCAA+AGG | + | chr7.3:82112360-82112379 | None:intergenic | 40.0% | |
| GTACATACCACAATGCTTAG+TGG | + | chr7.3:82112467-82112486 | None:intergenic | 40.0% | |
| GTTCAAAGTGAGGAACCTAT+TGG | - | chr7.3:82113144-82113163 | MS.gene97789:CDS | 40.0% | |
| TGCAATCTATAGGAATGGTG+GGG | - | chr7.3:82112999-82113018 | MS.gene97789:CDS | 40.0% | |
| TGCATAGTACAACCAAAGCA+TGG | + | chr7.3:82112074-82112093 | None:intergenic | 40.0% | |
| TGCTGCTGTATGCAATCTAT+AGG | - | chr7.3:82112989-82113008 | MS.gene97789:CDS | 40.0% | |
| TTACTTTCGGCAATGAACGT+GGG | - | chr7.3:82112332-82112351 | MS.gene97789:intron | 40.0% | |
| ! | GAACAAGGGTGATGATAATG+AGG | - | chr7.3:82113116-82113135 | MS.gene97789:intron | 40.0% |
| !! | CATTTCGTTCTTGGTGTTCT+TGG | - | chr7.3:82111873-82111892 | MS.gene97789:CDS | 40.0% |
| CACAAACCCATCCAAGAACT+TGG | + | chr7.3:82112415-82112434 | None:intergenic | 45.0% | |
| CAGCAATGCATATGTCCTTG+AGG | + | chr7.3:82112684-82112703 | None:intergenic | 45.0% | |
| CGGCATAGTTAGTGACTATG+AGG | + | chr7.3:82112373-82112392 | None:intergenic | 45.0% | |
| CTATGCACCAAGAGATGCAA+GGG | - | chr7.3:82112180-82112199 | MS.gene97789:intron | 45.0% | |
| CTTTGTTCAGCTCCATGCTT+TGG | - | chr7.3:82112059-82112078 | MS.gene97789:CDS | 45.0% | |
| TACTTTCGGCAATGAACGTG+GGG | - | chr7.3:82112333-82112352 | MS.gene97789:intron | 45.0% | |
| TCACATTGAGTGCCACAATG+TGG | - | chr7.3:82112641-82112660 | MS.gene97789:CDS | 45.0% | |
| TCGTGTCCAAGTTCTTGGAT+GGG | - | chr7.3:82112406-82112425 | MS.gene97789:CDS | 45.0% | |
| TCTATGCACCAAGAGATGCA+AGG | - | chr7.3:82112179-82112198 | MS.gene97789:intron | 45.0% | |
| TGGAGCTGAACAAAGCTACT+AGG | + | chr7.3:82112054-82112073 | None:intergenic | 45.0% | |
| TTCGTGTCCAAGTTCTTGGA+TGG | - | chr7.3:82112405-82112424 | MS.gene97789:CDS | 45.0% | |
| TTGAGTGCCACAATGTGGTT+TGG | - | chr7.3:82112646-82112665 | MS.gene97789:CDS | 45.0% | |
| ! | GTTACTTTCGGCAATGAACG+TGG | - | chr7.3:82112331-82112350 | MS.gene97789:intron | 45.0% |
| ACCGTAGCCAAACCACATTG+TGG | + | chr7.3:82112656-82112675 | None:intergenic | 50.0% | |
| CCAAGAACTTGGACACGAAG+TGG | + | chr7.3:82112404-82112423 | None:intergenic | 50.0% | |
| CCACTTCGTGTCCAAGTTCT+TGG | - | chr7.3:82112401-82112420 | MS.gene97789:CDS | 50.0% | |
| GTCACTAACTATGCCGTGCA+TGG | - | chr7.3:82112377-82112396 | MS.gene97789:CDS | 50.0% | |
| GTGCAGCTCCCAAATGTGTT+GGG | - | chr7.3:82112943-82112962 | MS.gene97789:CDS | 50.0% | |
| TGTGCAGCTCCCAAATGTGT+TGG | - | chr7.3:82112942-82112961 | MS.gene97789:CDS | 50.0% | |
| ! | GCCACAATGTGGTTTGGCTA+CGG | - | chr7.3:82112652-82112671 | MS.gene97789:CDS | 50.0% |
| ! | TGCAGCACCACTAAGCATTG+TGG | - | chr7.3:82112457-82112476 | MS.gene97789:intron | 50.0% |
| !! | AGTGAGGAACCTATTGGCTC+TGG | - | chr7.3:82113150-82113169 | MS.gene97789:CDS | 50.0% |
| GACACGAAGTGGACCATGCA+CGG | + | chr7.3:82112393-82112412 | None:intergenic | 55.0% | |
| TGGCTACGGTCTCTTCCTCA+AGG | - | chr7.3:82112666-82112685 | MS.gene97789:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 82111866 | 82113179 | 82111866 | ID=MS.gene97789 |
| chr7.3 | mRNA | 82111866 | 82113179 | 82111866 | ID=MS.gene97789.t1;Parent=MS.gene97789 |
| chr7.3 | exon | 82113143 | 82113179 | 82113143 | ID=MS.gene97789.t1.exon1;Parent=MS.gene97789.t1 |
| chr7.3 | CDS | 82113143 | 82113179 | 82113143 | ID=cds.MS.gene97789.t1;Parent=MS.gene97789.t1 |
| chr7.3 | exon | 82112844 | 82113054 | 82112844 | ID=MS.gene97789.t1.exon2;Parent=MS.gene97789.t1 |
| chr7.3 | CDS | 82112844 | 82113054 | 82112844 | ID=cds.MS.gene97789.t1;Parent=MS.gene97789.t1 |
| chr7.3 | exon | 82112567 | 82112728 | 82112567 | ID=MS.gene97789.t1.exon3;Parent=MS.gene97789.t1 |
| chr7.3 | CDS | 82112567 | 82112728 | 82112567 | ID=cds.MS.gene97789.t1;Parent=MS.gene97789.t1 |
| chr7.3 | exon | 82112343 | 82112462 | 82112343 | ID=MS.gene97789.t1.exon4;Parent=MS.gene97789.t1 |
| chr7.3 | CDS | 82112343 | 82112462 | 82112343 | ID=cds.MS.gene97789.t1;Parent=MS.gene97789.t1 |
| chr7.3 | exon | 82111866 | 82112096 | 82111866 | ID=MS.gene97789.t1.exon5;Parent=MS.gene97789.t1 |
| chr7.3 | CDS | 82111866 | 82112096 | 82111866 | ID=cds.MS.gene97789.t1;Parent=MS.gene97789.t1 |
| Gene Sequence |
| Protein sequence |
