Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97976.t1 | XP_013446213.1 | 97.5 | 120 | 3 | 0 | 1 | 120 | 1 | 120 | 1.00E-56 | 229.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97976.t1 | A0A072U339 | 97.5 | 120 | 3 | 0 | 1 | 120 | 1 | 120 | 7.4e-57 | 229.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene056482 | MS.gene97976 | 0.801767 | 7.71E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97976.t1 | MTR_8g070040 | 97.500 | 120 | 3 | 0 | 1 | 120 | 1 | 120 | 4.89e-80 | 231 |
| MS.gene97976.t1 | MTR_8g070015 | 96.460 | 113 | 4 | 0 | 1 | 113 | 1 | 113 | 2.66e-73 | 213 |
| MS.gene97976.t1 | MTR_7g065210 | 51.515 | 99 | 46 | 2 | 23 | 120 | 20 | 117 | 1.16e-26 | 95.9 |
| MS.gene97976.t1 | MTR_7g065160 | 51.579 | 95 | 44 | 2 | 27 | 120 | 24 | 117 | 3.23e-25 | 92.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene97976.t1 | AT2G37870 | 68.644 | 118 | 34 | 1 | 3 | 120 | 1 | 115 | 2.08e-50 | 155 |
| MS.gene97976.t1 | AT5G05960 | 45.902 | 122 | 58 | 3 | 1 | 120 | 1 | 116 | 7.29e-29 | 101 |
| MS.gene97976.t1 | AT3G53980 | 46.809 | 94 | 49 | 1 | 27 | 120 | 22 | 114 | 1.76e-27 | 97.8 |
| MS.gene97976.t1 | AT3G53980 | 46.809 | 94 | 49 | 1 | 27 | 120 | 22 | 114 | 1.76e-27 | 97.8 |
Find 25 sgRNAs with CRISPR-Local
Find 80 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGCATCAAGTGTGTTAATTT+TGG | 0.255637 | 8.4:-33103635 | MS.gene97976:CDS |
| AGCTAGTCTAAGTCCATGTT+TGG | 0.350440 | 8.4:-33103563 | MS.gene97976:CDS |
| TTCCTAATGTTGCATCTCTT+AGG | 0.378053 | 8.4:+33103388 | None:intergenic |
| TGGGTGCTGTACGTAATGTT+AGG | 0.392658 | 8.4:-33103543 | MS.gene97976:CDS |
| GAATGTGGAAGGACACCAAT+TGG | 0.434211 | 8.4:-33103595 | MS.gene97976:CDS |
| GACAATAGTGATGGAGTTCA+AGG | 0.446807 | 8.4:-33103695 | None:intergenic |
| AGAACAGAACAAAGACATCT+TGG | 0.469439 | 8.4:+33103466 | None:intergenic |
| CTTCCACATTTCTTTCCAGC+AGG | 0.469545 | 8.4:+33103361 | None:intergenic |
| CCAACTCTAGCACAACAAAC+TGG | 0.478057 | 8.4:+33103508 | None:intergenic |
| CTAGCTGCTGCTGACCCAAT+TGG | 0.492373 | 8.4:+33103580 | None:intergenic |
| TTCCTAAGAGATGCAACATT+AGG | 0.493031 | 8.4:-33103390 | MS.gene97976:CDS |
| GGGTGCTGTACGTAATGTTA+GGG | 0.506383 | 8.4:-33103542 | MS.gene97976:CDS |
| CCAGTTTGTTGTGCTAGAGT+TGG | 0.508251 | 8.4:-33103508 | MS.gene97976:CDS |
| AGTGAGGCAGCTGGAGAATG+TGG | 0.508656 | 8.4:-33103610 | MS.gene97976:CDS |
| CACATTTCTTTCCAGCAGGT+CGG | 0.514268 | 8.4:+33103365 | None:intergenic |
| AATGTGGAAGGACACCAATT+GGG | 0.523864 | 8.4:-33103594 | MS.gene97976:CDS |
| AACATTAGGAACCGACCTGC+TGG | 0.527493 | 8.4:-33103376 | MS.gene97976:CDS |
| GCTAGTCTAAGTCCATGTTT+GGG | 0.544347 | 8.4:-33103562 | MS.gene97976:CDS |
| GGAACAGTGATAGCAATAGC+AGG | 0.582273 | 8.4:+33103409 | None:intergenic |
| TTACGTACAGCACCCAAACA+TGG | 0.602239 | 8.4:+33103550 | None:intergenic |
| CGACCTGCTGGAAAGAAATG+TGG | 0.609362 | 8.4:-33103364 | MS.gene97976:CDS |
| AGGCAGCTGGAGAATGTGGA+AGG | 0.611703 | 8.4:-33103606 | MS.gene97976:CDS |
| AAAACATCACCACTGTTGCA+AGG | 0.620969 | 8.4:+33103662 | None:intergenic |
| ACTCTAGCACAACAAACTGG+TGG | 0.633479 | 8.4:+33103511 | None:intergenic |
| AAGAACTTTCCTTGCAACAG+TGG | 0.633689 | 8.4:-33103671 | MS.gene97976:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | CTTTTTTTATTTTATTTACA+GGG | - | chr8.4:33103670-33103689 | MS.gene97976:CDS | 10.0% |
| !! | AATAAAATAAAAAAAGGACA+AGG | + | chr8.4:33103666-33103685 | None:intergenic | 15.0% |
| !! | CCTGTAAATAAAATAAAAAA+AGG | + | chr8.4:33103672-33103691 | None:intergenic | 15.0% |
| !!! | CCTTTTTTTATTTTATTTAC+AGG | - | chr8.4:33103669-33103688 | MS.gene97976:CDS | 15.0% |
| !! | ATGCAATATATACTCAAAAT+AGG | - | chr8.4:33103233-33103252 | MS.gene97976:intron | 20.0% |
| !! | CAAAAATATGAATCAATGAA+AGG | + | chr8.4:33103098-33103117 | None:intergenic | 20.0% |
| !! | TACTAATGTCATAAACATTA+TGG | - | chr8.4:33103583-33103602 | MS.gene97976:CDS | 20.0% |
| !!! | CTTTCATTGATTCATATTTT+TGG | - | chr8.4:33103096-33103115 | MS.gene97976:intron | 20.0% |
| !!! | TAAGCAAAAAGTAAGTTTTT+AGG | + | chr8.4:33103417-33103436 | None:intergenic | 20.0% |
| !!! | TATTTTGAGTATATATTGCA+TGG | + | chr8.4:33103234-33103253 | None:intergenic | 20.0% |
| ! | CATTGAATAATTATTGCATC+TGG | - | chr8.4:33103353-33103372 | MS.gene97976:intron | 25.0% |
| ! | CATTGAATGATTAATGTACA+TGG | + | chr8.4:33103392-33103411 | None:intergenic | 25.0% |
| ! | GATATAAAACTGAAGCAAAA+AGG | - | chr8.4:33103547-33103566 | MS.gene97976:CDS | 25.0% |
| !! | AGATGTTTTCAAAACTAAGA+TGG | + | chr8.4:33103072-33103091 | None:intergenic | 25.0% |
| !! | TTTTTGCTTATAAATGTGAC+CGG | + | chr8.4:33103318-33103337 | None:intergenic | 25.0% |
| !! | TTTTTGCTTATATATGTGAC+CGG | - | chr8.4:33103427-33103446 | MS.gene97976:CDS | 25.0% |
| !!! | TCATTTAGACTTTGTTGTTA+GGG | + | chr8.4:33103200-33103219 | None:intergenic | 25.0% |
| !!! | TTCATTTAGACTTTGTTGTT+AGG | + | chr8.4:33103201-33103220 | None:intergenic | 25.0% |
| AACTAGATGACATCTTCTAT+TGG | - | chr8.4:33103464-33103483 | MS.gene97976:CDS | 30.0% | |
| CTGACAATGTGTTTATAAGT+GGG | - | chr8.4:33102965-33102984 | MS.gene97976:intron | 30.0% | |
| TGACAATGTGTTTATAAGTG+GGG | - | chr8.4:33102966-33102985 | MS.gene97976:intron | 30.0% | |
| ! | TGTGCACATTTTGCAAAATT+TGG | - | chr8.4:33103520-33103539 | MS.gene97976:CDS | 30.0% |
| ! | TGTTTTCAAAACTAAGATGG+AGG | + | chr8.4:33103069-33103088 | None:intergenic | 30.0% |
| !!! | ATCTTAGCTTGTTTTGCAAT+AGG | + | chr8.4:33102727-33102746 | None:intergenic | 30.0% |
| AGAACAGAACAAAGACATCT+TGG | + | chr8.4:33102697-33102716 | None:intergenic | 35.0% | |
| CCACTTATAAACACATTGTC+AGG | + | chr8.4:33102967-33102986 | None:intergenic | 35.0% | |
| CCTGACAATGTGTTTATAAG+TGG | - | chr8.4:33102964-33102983 | MS.gene97976:intron | 35.0% | |
| CTGAAGCAAAAAGGAGTTTA+TGG | - | chr8.4:33103556-33103575 | MS.gene97976:CDS | 35.0% | |
| GACAATGTGTTTATAAGTGG+GGG | - | chr8.4:33102967-33102986 | MS.gene97976:intron | 35.0% | |
| GTGTTTAGAGATTGTAGAAG+AGG | - | chr8.4:33103160-33103179 | MS.gene97976:intron | 35.0% | |
| TGATACCATCTTAGAAATCG+TGG | + | chr8.4:33103040-33103059 | None:intergenic | 35.0% | |
| TTCCTAAGAGATGCAACATT+AGG | - | chr8.4:33102770-33102789 | MS.gene97976:intron | 35.0% | |
| TTCCTAATGTTGCATCTCTT+AGG | + | chr8.4:33102775-33102794 | None:intergenic | 35.0% | |
| TTTCAAAACTAAGATGGAGG+AGG | + | chr8.4:33103066-33103085 | None:intergenic | 35.0% | |
| TTTCAGAAAAATACTCCCTC+CGG | - | chr8.4:33103296-33103315 | MS.gene97976:intron | 35.0% | |
| ! | GGCATCAAGTGTGTTAATTT+TGG | - | chr8.4:33102525-33102544 | MS.gene97976:intron | 35.0% |
| !! | AACAGTGGTGATGTTTTTGT+TGG | - | chr8.4:33102504-33102523 | MS.gene97976:intron | 35.0% |
| !! | TGTGTTAATTTTGGAGAGTG+AGG | - | chr8.4:33102534-33102553 | MS.gene97976:intron | 35.0% |
| AAAACATCACCACTGTTGCA+AGG | + | chr8.4:33102501-33102520 | None:intergenic | 40.0% | |
| AAGAACTTTCCTTGCAACAG+TGG | - | chr8.4:33102489-33102508 | MS.gene97976:intron | 40.0% | |
| AATGTGGAAGGACACCAATT+GGG | - | chr8.4:33102566-33102585 | MS.gene97976:intron | 40.0% | |
| ACCATCTTAGAAATCGTGGT+TGG | + | chr8.4:33103036-33103055 | None:intergenic | 40.0% | |
| CCATCTTAGAAATCGTGGTT+GGG | + | chr8.4:33103035-33103054 | None:intergenic | 40.0% | |
| CTAACACAATCCCACAAAAC+CGG | + | chr8.4:33103011-33103030 | None:intergenic | 40.0% | |
| TAGTTAATCACTACTCCCTC+CGG | + | chr8.4:33103449-33103468 | None:intergenic | 40.0% | |
| TATGTTCTGCTTGTGTCAAG+TGG | + | chr8.4:33103497-33103516 | None:intergenic | 40.0% | |
| TGCTTATAAATGTGACCGGA+GGG | + | chr8.4:33103314-33103333 | None:intergenic | 40.0% | |
| TGCTTATATATGTGACCGGA+GGG | - | chr8.4:33103431-33103450 | MS.gene97976:CDS | 40.0% | |
| TTGCTTATAAATGTGACCGG+AGG | + | chr8.4:33103315-33103334 | None:intergenic | 40.0% | |
| TTGCTTATATATGTGACCGG+AGG | - | chr8.4:33103430-33103449 | MS.gene97976:CDS | 40.0% | |
| ! | AGCTAGTCTAAGTCCATGTT+TGG | - | chr8.4:33102597-33102616 | MS.gene97976:intron | 40.0% |
| ! | GCTAGTCTAAGTCCATGTTT+GGG | - | chr8.4:33102598-33102617 | MS.gene97976:intron | 40.0% |
| ACTCTAGCACAACAAACTGG+TGG | + | chr8.4:33102652-33102671 | None:intergenic | 45.0% | |
| CACATTTCTTTCCAGCAGGT+CGG | + | chr8.4:33102798-33102817 | None:intergenic | 45.0% | |
| CCAACTCTAGCACAACAAAC+TGG | + | chr8.4:33102655-33102674 | None:intergenic | 45.0% | |
| CTAAGAGTCCCACATCAGAT+AGG | - | chr8.4:33102935-33102954 | MS.gene97976:intron | 45.0% | |
| CTTCCACATTTCTTTCCAGC+AGG | + | chr8.4:33102802-33102821 | None:intergenic | 45.0% | |
| GAATGTGGAAGGACACCAAT+TGG | - | chr8.4:33102565-33102584 | MS.gene97976:intron | 45.0% | |
| GACTCTTAGCACGCACATTA+AGG | + | chr8.4:33102924-33102943 | None:intergenic | 45.0% | |
| GGAACAGTGATAGCAATAGC+AGG | + | chr8.4:33102754-33102773 | None:intergenic | 45.0% | |
| GTCCCACATCAGATAGGATA+AGG | - | chr8.4:33102941-33102960 | MS.gene97976:intron | 45.0% | |
| TTACGTACAGCACCCAAACA+TGG | + | chr8.4:33102613-33102632 | None:intergenic | 45.0% | |
| ! | AGGCCTTATCCTATCTGATG+TGG | + | chr8.4:33102947-33102966 | None:intergenic | 45.0% |
| ! | CCCAACCACGATTTCTAAGA+TGG | - | chr8.4:33103032-33103051 | MS.gene97976:intron | 45.0% |
| ! | GGCCTTATCCTATCTGATGT+GGG | + | chr8.4:33102946-33102965 | None:intergenic | 45.0% |
| ! | GGGTGCTGTACGTAATGTTA+GGG | - | chr8.4:33102618-33102637 | MS.gene97976:intron | 45.0% |
| ! | TGGGTGCTGTACGTAATGTT+AGG | - | chr8.4:33102617-33102636 | MS.gene97976:intron | 45.0% |
| !! | ACCTCTTAAGCCGGTTTTGT+GGG | - | chr8.4:33102998-33103017 | MS.gene97976:intron | 45.0% |
| !! | CCAGTTTGTTGTGCTAGAGT+TGG | - | chr8.4:33102652-33102671 | MS.gene97976:intron | 45.0% |
| !! | CGGTTTTGTGGGATTGTGTT+AGG | - | chr8.4:33103009-33103028 | MS.gene97976:intron | 45.0% |
| AACATTAGGAACCGACCTGC+TGG | - | chr8.4:33102784-33102803 | MS.gene97976:intron | 50.0% | |
| CGACCTGCTGGAAAGAAATG+TGG | - | chr8.4:33102796-33102815 | MS.gene97976:intron | 50.0% | |
| GCAATCCTCACCTCTTAAGC+CGG | - | chr8.4:33102989-33103008 | MS.gene97976:intron | 50.0% | |
| ! | CAAAACCGGCTTAAGAGGTG+AGG | + | chr8.4:33102997-33103016 | None:intergenic | 50.0% |
| ! | TCCCACAAAACCGGCTTAAG+AGG | + | chr8.4:33103002-33103021 | None:intergenic | 50.0% |
| !! | ATTTTGGAGAGTGAGGCAGC+TGG | - | chr8.4:33102541-33102560 | MS.gene97976:intron | 50.0% |
| !! | CACCTCTTAAGCCGGTTTTG+TGG | - | chr8.4:33102997-33103016 | MS.gene97976:intron | 50.0% |
| AGGCAGCTGGAGAATGTGGA+AGG | - | chr8.4:33102554-33102573 | MS.gene97976:intron | 55.0% | |
| AGTGAGGCAGCTGGAGAATG+TGG | - | chr8.4:33102550-33102569 | MS.gene97976:intron | 55.0% | |
| CTAGCTGCTGCTGACCCAAT+TGG | + | chr8.4:33102583-33102602 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.4 | gene | 33102475 | 33103707 | 33102475 | ID=MS.gene97976 |
| chr8.4 | mRNA | 33102475 | 33103707 | 33102475 | ID=MS.gene97976.t1;Parent=MS.gene97976 |
| chr8.4 | exon | 33103362 | 33103707 | 33103362 | ID=MS.gene97976.t1.exon1;Parent=MS.gene97976.t1 |
| chr8.4 | CDS | 33103362 | 33103707 | 33103362 | ID=cds.MS.gene97976.t1;Parent=MS.gene97976.t1 |
| chr8.4 | exon | 33102475 | 33102491 | 33102475 | ID=MS.gene97976.t1.exon2;Parent=MS.gene97976.t1 |
| chr8.4 | CDS | 33102475 | 33102491 | 33102475 | ID=cds.MS.gene97976.t1;Parent=MS.gene97976.t1 |
| Gene Sequence |
| Protein sequence |
