Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98161.t1 | XP_024636865.1 | 86.3 | 277 | 37 | 1 | 1 | 277 | 32 | 307 | 1.50E-130 | 475.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98161.t1 | Q94JZ8 | 56.3 | 199 | 72 | 3 | 32 | 216 | 116 | 313 | 3.1e-57 | 223.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98161.t1 | A0A396I635 | 86.3 | 277 | 37 | 1 | 1 | 277 | 32 | 307 | 1.0e-130 | 475.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene055357 | MS.gene98161 | 0.823212 | 1.56E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98161.t1 | MTR_4g006220 | 85.560 | 277 | 37 | 2 | 1 | 277 | 32 | 305 | 6.31e-174 | 482 |
| MS.gene98161.t1 | MTR_4g006220 | 82.414 | 290 | 37 | 2 | 1 | 277 | 32 | 320 | 1.03e-173 | 482 |
| MS.gene98161.t1 | MTR_4g006220 | 85.520 | 221 | 31 | 1 | 1 | 221 | 32 | 251 | 9.49e-140 | 394 |
| MS.gene98161.t1 | MTR_8g070760 | 75.000 | 248 | 51 | 6 | 30 | 269 | 120 | 364 | 1.29e-124 | 363 |
| MS.gene98161.t1 | MTR_4g006310 | 61.372 | 277 | 80 | 5 | 1 | 277 | 37 | 286 | 1.80e-104 | 305 |
| MS.gene98161.t1 | MTR_4g011870 | 59.009 | 222 | 82 | 3 | 1 | 217 | 32 | 249 | 5.83e-87 | 262 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98161.t1 | AT1G14570 | 54.852 | 237 | 87 | 5 | 32 | 249 | 116 | 351 | 6.47e-78 | 244 |
| MS.gene98161.t1 | AT1G14570 | 54.852 | 237 | 87 | 5 | 32 | 249 | 116 | 351 | 6.47e-78 | 244 |
| MS.gene98161.t1 | AT1G14570 | 63.043 | 184 | 63 | 2 | 71 | 249 | 147 | 330 | 2.32e-77 | 242 |
| MS.gene98161.t1 | AT1G14570 | 63.043 | 184 | 63 | 2 | 71 | 249 | 147 | 330 | 2.32e-77 | 242 |
| MS.gene98161.t1 | AT4G14245 | 47.857 | 140 | 70 | 2 | 78 | 216 | 25 | 162 | 2.35e-39 | 140 |
| MS.gene98161.t1 | AT4G14250 | 34.091 | 220 | 84 | 6 | 1 | 207 | 35 | 206 | 2.43e-29 | 114 |
Find 63 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATACTTCTAATAAACTTTC+AGG | 0.200225 | 4.1:-88967703 | None:intergenic |
| GATGGACTAGAATTTCTTCT+AGG | 0.279174 | 4.1:-88967923 | None:intergenic |
| TATGCAGGACAAGTGGTTAT+TGG | 0.289865 | 4.1:+88957615 | MS.gene98161:CDS |
| TTAGTACAAACTTCATATTC+TGG | 0.302758 | 4.1:+88957809 | MS.gene98161:CDS |
| CTCCTTACTTTACTGTGAAC+AGG | 0.362529 | 4.1:+88956147 | MS.gene98161:CDS |
| AACAGGAAACAACGAGGGTT+TGG | 0.388085 | 4.1:+88956164 | MS.gene98161:CDS |
| CGTGAAAGATTCAAGTGAAA+TGG | 0.422161 | 4.1:+88968238 | MS.gene98161:CDS |
| TCACAGTAAAGTAAGGAGTT+TGG | 0.423266 | 4.1:-88956142 | None:intergenic |
| AGCTTCCTCAAGATTCCAAT+TGG | 0.424401 | 4.1:-88955708 | None:intergenic |
| AAGGTATTGTGGTGATCCTT+AGG | 0.425274 | 4.1:-88967887 | None:intergenic |
| AATAAAGATGAAAATGTTGC+TGG | 0.436314 | 4.1:+88968272 | MS.gene98161:CDS |
| GAAAGCTTAATCGGGATACA+TGG | 0.439368 | 4.1:+88957761 | MS.gene98161:intron |
| TCTAGTCCATCAAAATCAAC+AGG | 0.440553 | 4.1:+88967935 | MS.gene98161:CDS |
| CATCTTTGTGCAGAGGATCA+AGG | 0.442158 | 4.1:+88956095 | MS.gene98161:intron |
| TTCCTGTTCACAGTAAAGTA+AGG | 0.451369 | 4.1:-88956149 | None:intergenic |
| CTGCTACCATTCCTTGATGC+TGG | 0.455289 | 4.1:+88967863 | MS.gene98161:CDS |
| GAGTTTCTCAAACTCCATTA+CGG | 0.456836 | 4.1:-88968312 | None:intergenic |
| AGGTTCAACCATTCCGCCCC+AGG | 0.457021 | 4.1:-88967683 | None:intergenic |
| GGATCAATAATAAGCACAAC+AGG | 0.471577 | 4.1:-88967637 | None:intergenic |
| ATCTTTGTAGATTCAGATGC+GGG | 0.488024 | 4.1:+88968164 | MS.gene98161:intron |
| CCATCGAGCAATAGATGTAA+AGG | 0.489576 | 4.1:-88956262 | None:intergenic |
| ATTCCTTGATGCTGGTCCTA+AGG | 0.491407 | 4.1:+88967871 | MS.gene98161:CDS |
| CTTATTATTGATCCCATCAC+TGG | 0.493107 | 4.1:+88967646 | MS.gene98161:CDS |
| GAAAAGTGCTGCTTCTATGC+AGG | 0.495815 | 4.1:+88957600 | MS.gene98161:CDS |
| GCTCTTGACTGCTACCTCAC+TGG | 0.506718 | 4.1:+88955728 | MS.gene98161:CDS |
| CAATCCTGCGTCTGCGTCGA+CGG | 0.506944 | 4.1:+88955768 | MS.gene98161:CDS |
| TACAGCAGAGAGTTCTCAGC+AGG | 0.508617 | 4.1:+88956213 | MS.gene98161:CDS |
| TACAAACTTCATATTCTGGC+AGG | 0.512424 | 4.1:+88957813 | MS.gene98161:CDS |
| GCTCGATGGCTCTTTCTATG+AGG | 0.528694 | 4.1:+88956276 | MS.gene98161:CDS |
| GATTCAGATGCGGGTGAAGA+GGG | 0.530150 | 4.1:+88968173 | MS.gene98161:intron |
| TGAGGAAGTTCAAAGAGCGC+TGG | 0.549875 | 4.1:+88968199 | MS.gene98161:CDS |
| GATGCGCTCCTGGGGCGGAA+TGG | 0.552167 | 4.1:+88967675 | MS.gene98161:CDS |
| CTTGCAAGAAACAACCGTAA+TGG | 0.557258 | 4.1:+88968298 | MS.gene98161:CDS |
| GGCTTCCAATTGGAATCTTG+AGG | 0.557321 | 4.1:+88955703 | None:intergenic |
| CCTTTACATCTATTGCTCGA+TGG | 0.561806 | 4.1:+88956262 | MS.gene98161:CDS |
| AAAGATTCAAGTGAAATGGC+TGG | 0.568867 | 4.1:+88968242 | MS.gene98161:CDS |
| AGGGTTTGGAAAACAGAGCT+AGG | 0.586416 | 4.1:+88956178 | MS.gene98161:CDS |
| AGATTCAGATGCGGGTGAAG+AGG | 0.588759 | 4.1:+88968172 | MS.gene98161:intron |
| GATCCTTAGGACCAGCATCA+AGG | 0.594251 | 4.1:-88967874 | None:intergenic |
| TATACCGTCGACGCAGACGC+AGG | 0.596884 | 4.1:-88955772 | None:intergenic |
| AAAGCTTAATCGGGATACAT+GGG | 0.597473 | 4.1:+88957762 | MS.gene98161:intron |
| TTCAGATGCGGGTGAAGAGG+GGG | 0.598919 | 4.1:+88968175 | MS.gene98161:CDS |
| CATGTGTGAGCTGAATTCCT+TGG | 0.600082 | 4.1:-88957653 | None:intergenic |
| TGTAATCAGTGCAGACATTG+TGG | 0.601498 | 4.1:-88967600 | None:intergenic |
| AGAACTCTCTGCTGTAGAAG+TGG | 0.606299 | 4.1:-88956206 | None:intergenic |
| TGTCTGCACTGATTACAGAC+TGG | 0.609010 | 4.1:+88967606 | MS.gene98161:CDS |
| CAAGGAATTCAGCTCACACA+TGG | 0.615182 | 4.1:+88957654 | MS.gene98161:CDS |
| CTGCTTCTATGCAGGACAAG+TGG | 0.615316 | 4.1:+88957608 | MS.gene98161:CDS |
| TCGAGCAATAGATGTAAAGG+AGG | 0.619816 | 4.1:-88956259 | None:intergenic |
| AATCTTTGTAGATTCAGATG+CGG | 0.624918 | 4.1:+88968163 | MS.gene98161:intron |
| TTAGGACCAGCATCAAGGAA+TGG | 0.632117 | 4.1:-88967869 | None:intergenic |
| TCTAGGAAACTTGTGAGACA+AGG | 0.634489 | 4.1:-88967906 | None:intergenic |
| ACTTGTGAGACAAGGTATTG+TGG | 0.639244 | 4.1:-88967898 | None:intergenic |
| ATTCAGATGCGGGTGAAGAG+GGG | 0.648757 | 4.1:+88968174 | MS.gene98161:CDS |
| ACTCTCTGCTGTAGAAGTGG+CGG | 0.651990 | 4.1:-88956203 | None:intergenic |
| TTGTGCAGAGGATCAAGGAG+TGG | 0.660474 | 4.1:+88956100 | MS.gene98161:intron |
| CTGTGAACAGGAAACAACGA+GGG | 0.660652 | 4.1:+88956159 | MS.gene98161:CDS |
| CGAGCAATAGATGTAAAGGA+GGG | 0.663425 | 4.1:-88956258 | None:intergenic |
| GATTCAAGTGAAATGGCTGG+AGG | 0.685148 | 4.1:+88968245 | MS.gene98161:CDS |
| GGTGAACATACAATCTACCA+AGG | 0.686385 | 4.1:+88957636 | MS.gene98161:CDS |
| GTATATGATGATACAACTGA+AGG | 0.707733 | 4.1:+88967577 | MS.gene98161:CDS |
| GATCATCATAATCTCCAGTG+AGG | 0.716475 | 4.1:-88955742 | None:intergenic |
| ACTGTGAACAGGAAACAACG+AGG | 0.783493 | 4.1:+88956158 | MS.gene98161:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 88955704 | 88968343 | 88955704 | ID=MS.gene98161 |
| chr4.1 | mRNA | 88955704 | 88968343 | 88955704 | ID=MS.gene98161.t1;Parent=MS.gene98161 |
| chr4.1 | exon | 88955704 | 88955789 | 88955704 | ID=MS.gene98161.t1.exon1;Parent=MS.gene98161.t1 |
| chr4.1 | CDS | 88955704 | 88955789 | 88955704 | ID=cds.MS.gene98161.t1;Parent=MS.gene98161.t1 |
| chr4.1 | exon | 88956108 | 88956297 | 88956108 | ID=MS.gene98161.t1.exon2;Parent=MS.gene98161.t1 |
| chr4.1 | CDS | 88956108 | 88956297 | 88956108 | ID=cds.MS.gene98161.t1;Parent=MS.gene98161.t1 |
| chr4.1 | exon | 88957598 | 88957675 | 88957598 | ID=MS.gene98161.t1.exon3;Parent=MS.gene98161.t1 |
| chr4.1 | CDS | 88957598 | 88957675 | 88957598 | ID=cds.MS.gene98161.t1;Parent=MS.gene98161.t1 |
| chr4.1 | exon | 88957766 | 88957834 | 88957766 | ID=MS.gene98161.t1.exon4;Parent=MS.gene98161.t1 |
| chr4.1 | CDS | 88957766 | 88957834 | 88957766 | ID=cds.MS.gene98161.t1;Parent=MS.gene98161.t1 |
| chr4.1 | exon | 88967577 | 88967720 | 88967577 | ID=MS.gene98161.t1.exon5;Parent=MS.gene98161.t1 |
| chr4.1 | CDS | 88967577 | 88967720 | 88967577 | ID=cds.MS.gene98161.t1;Parent=MS.gene98161.t1 |
| chr4.1 | exon | 88967860 | 88967956 | 88967860 | ID=MS.gene98161.t1.exon6;Parent=MS.gene98161.t1 |
| chr4.1 | CDS | 88967860 | 88967956 | 88967860 | ID=cds.MS.gene98161.t1;Parent=MS.gene98161.t1 |
| chr4.1 | exon | 88968174 | 88968343 | 88968174 | ID=MS.gene98161.t1.exon7;Parent=MS.gene98161.t1 |
| chr4.1 | CDS | 88968174 | 88968343 | 88968174 | ID=cds.MS.gene98161.t1;Parent=MS.gene98161.t1 |
| Gene Sequence |
| Protein sequence |
