AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene98307


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene98307.t1 MTR_3g074830 97.476 317 7 1 1 316 29 345 0.0 632
MS.gene98307.t1 MTR_5g064360 70.533 319 88 4 1 316 1 316 7.47e-162 454
MS.gene98307.t1 MTR_5g464340 71.473 319 84 5 1 316 1 315 2.68e-161 452
MS.gene98307.t1 MTR_5g064340 71.473 319 84 5 1 316 1 315 2.68e-161 452
MS.gene98307.t1 MTR_5g064345 72.115 312 79 5 8 316 9 315 5.21e-160 449
MS.gene98307.t1 MTR_3g074860 70.470 298 74 4 19 316 21 304 6.91e-150 423
MS.gene98307.t1 MTR_3g074890 69.799 298 76 4 19 316 21 304 2.56e-148 419
MS.gene98307.t1 MTR_4g094240 61.512 291 99 3 26 316 23 300 2.53e-127 365
MS.gene98307.t1 MTR_2g009070 54.397 307 122 6 11 316 15 304 8.34e-120 347
MS.gene98307.t1 MTR_1g076470 37.818 275 151 9 38 301 64 329 7.96e-43 150
MS.gene98307.t1 MTR_4g033790 37.993 279 152 9 38 305 65 333 2.38e-42 149
MS.gene98307.t1 MTR_8g071310 88.235 68 8 0 249 316 18 85 1.29e-35 124
MS.gene98307.t1 MTR_8g088900 74.444 90 23 0 227 316 283 372 9.97e-35 130
MS.gene98307.t1 MTR_4g013230 92.308 65 2 1 252 316 79 140 2.48e-33 120
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene98307.t1 AT4G08950 73.090 301 74 4 20 316 17 314 6.49e-157 441
MS.gene98307.t1 AT1G35140 63.226 310 106 4 8 315 5 308 2.63e-138 394
MS.gene98307.t1 AT5G64260 57.680 319 118 6 1 316 1 305 1.58e-127 366
MS.gene98307.t1 AT5G09440 57.509 273 88 6 46 316 32 278 4.66e-101 298
MS.gene98307.t1 AT3G02970 34.228 298 161 6 48 314 37 330 2.16e-49 167
MS.gene98307.t1 AT5G51550 39.024 287 154 9 38 313 55 331 8.63e-47 161
MS.gene98307.t1 AT2G35150 36.533 323 172 12 14 312 3 316 4.17e-41 145
MS.gene98307.t1 AT2G17230 35.473 296 168 10 32 313 71 357 4.41e-40 144

Find 66 sgRNAs with CRISPR-Local

Find 76 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAGCTTGTCCTGGTGTTTA+TGG 0.171889 3.1:+61193667 MS.gene98307:CDS
ATCAAGAATTTGCTTGTTTA+GGG 0.208436 3.1:-61193221 None:intergenic
CATCAAGAATTTGCTTGTTT+AGG 0.214856 3.1:-61193222 None:intergenic
GTTCACCTTTGGAAGCTAAT+TGG 0.254782 3.1:-61193288 None:intergenic
AGATGGAATGGTGATAAATT+TGG 0.289948 3.1:+61193561 MS.gene98307:CDS
TTTGAGAAGAGATTGAAAAT+TGG 0.300887 3.1:-61192894 None:intergenic
CTTGATGAGAATTACTCATT+AGG 0.306480 3.1:+61193238 MS.gene98307:CDS
CCATTTCACCAACCAATTTA+CGG 0.318307 3.1:+61193493 MS.gene98307:CDS
TTGCAAAATTCGCTTACATT+TGG 0.318352 3.1:+61193428 MS.gene98307:CDS
GTGCTTTGTGGACCGTAAAT+TGG 0.326346 3.1:-61193505 None:intergenic
TGCAAAATTCGCTTACATTT+GGG 0.327906 3.1:+61193429 MS.gene98307:CDS
AAATCTCAGTCAATTTAATA+TGG 0.337979 3.1:+61193032 MS.gene98307:CDS
ACACGGTTCTTCTTCTTCAA+TGG 0.340114 3.1:+61193387 MS.gene98307:CDS
TTTGGAAATGGTTACTTTCA+AGG 0.342445 3.1:+61193616 MS.gene98307:CDS
TTCAATGCTCATGGTGATAA+TGG 0.347620 3.1:+61193751 MS.gene98307:CDS
AACAACGATGTTGGACTAGA+TGG 0.355037 3.1:+61193544 MS.gene98307:CDS
TTGCTTGTTGATTCTACTAC+TGG 0.362064 3.1:+61193721 MS.gene98307:CDS
CCGTAAATTGGTTGGTGAAA+TGG 0.399220 3.1:-61193493 None:intergenic
GGAATCTTTGTGTTCACCTT+TGG 0.401182 3.1:-61193299 None:intergenic
GGAACAGCAACAAACCCTTT+TGG 0.401625 3.1:+61193598 MS.gene98307:CDS
CACAACGGTCCATTACTCTA+TGG 0.410127 3.1:+61193007 MS.gene98307:CDS
TGAAAGTAACCATTTCCAAA+AGG 0.429612 3.1:-61193613 None:intergenic
GCAACCAATGGTGTGCTTTG+TGG 0.435268 3.1:-61193517 None:intergenic
GAAAGTAACCATTTCCAAAA+GGG 0.440576 3.1:-61193612 None:intergenic
GTTGAGCAAGATAATGTAGA+AGG 0.441408 3.1:-61193805 None:intergenic
GCTGAAACTGAAGGTTGGTT+TGG 0.443189 3.1:-61193130 None:intergenic
TCTAGTCCAACATCGTTGTT+TGG 0.445960 3.1:-61193541 None:intergenic
GAAGCTGCTTCAGCTTGTCC+TGG 0.453010 3.1:+61193658 MS.gene98307:CDS
AGATGTTAGTACAACGTTGA+TGG 0.462886 3.1:-61193320 None:intergenic
AGTTTCTAGCAGCTGAAGAA+AGG 0.492873 3.1:-61192943 None:intergenic
TGTCCTGGTGTTTATGGTAA+AGG 0.498489 3.1:+61193673 MS.gene98307:CDS
ACCATGCTGAAACTGAAGGT+TGG 0.500598 3.1:-61193135 None:intergenic
GGTGCTTACCCTGGTTATGC+TGG 0.504722 3.1:+61193694 MS.gene98307:CDS
TCTTCAATGGAATCCAGAAA+AGG 0.511852 3.1:+61193400 MS.gene98307:CDS
TCAGTCAATTTAATATGGTA+TGG 0.514800 3.1:+61193037 MS.gene98307:CDS
AGCAAATCACCAGCATAACC+AGG 0.517128 3.1:-61193703 None:intergenic
GTTGCACCAAACAACGATGT+TGG 0.520520 3.1:+61193535 MS.gene98307:CDS
CGGTCCACAAAGCACACCAT+TGG 0.532282 3.1:+61193513 MS.gene98307:CDS
AGTGTCCAGGTCTATGTGCT+TGG 0.546729 3.1:+61193470 MS.gene98307:CDS
AATGGAATCCAGAAAAGGTA+AGG 0.547288 3.1:+61193405 MS.gene98307:CDS
AAATTCGCTTACATTTGGGT+TGG 0.549048 3.1:+61193433 MS.gene98307:CDS
ATGCTCATGGTGATAATGGA+AGG 0.567528 3.1:+61193755 MS.gene98307:CDS
ATTATCCAATTAGCTTCCAA+AGG 0.570977 3.1:+61193283 MS.gene98307:CDS
TTCCACCATGCTGAAACTGA+AGG 0.570995 3.1:-61193139 None:intergenic
AACTCAGAAACACAGTGTCC+AGG 0.573210 3.1:+61193457 MS.gene98307:CDS
GTAGAAGGATCATACAAAGC+AGG 0.575060 3.1:-61193790 None:intergenic
GGTAATGAAGAAGCTGAGAT+TGG 0.577764 3.1:-61192985 None:intergenic
TATGGTAAAGGTGCTTACCC+TGG 0.580611 3.1:+61193685 MS.gene98307:CDS
TGAAGATGGTGAAGAAAGTG+AGG 0.583061 3.1:-61193104 None:intergenic
ATTGCAATGTTCTTCAAACA+AGG 0.583966 3.1:-61193828 None:intergenic
TTTGTGGACCGTAAATTGGT+TGG 0.588949 3.1:-61193501 None:intergenic
GGTGCTAGTTTCAATGCTCA+TGG 0.589796 3.1:+61193742 MS.gene98307:CDS
AACCTTCAGTTTCAGCATGG+TGG 0.591696 3.1:+61193137 MS.gene98307:CDS
TTGCAATGTTCTTCAAACAA+GGG 0.601668 3.1:-61193827 None:intergenic
GCTTCAAGAGGAGCTTCAGA+AGG 0.602180 3.1:-61193640 None:intergenic
ATGGACCGATGTGGCACACA+CGG 0.610497 3.1:+61193370 MS.gene98307:CDS
TCGTTGTTTGGTGCAACCAA+TGG 0.613248 3.1:-61193529 None:intergenic
AAGAACCGTGTGTGCCACAT+CGG 0.625233 3.1:-61193375 None:intergenic
CAAGCTGAAGCAGCTTCAAG+AGG 0.626827 3.1:-61193652 None:intergenic
ACCAACCTTCAGTTTCAGCA+TGG 0.631067 3.1:+61193134 MS.gene98307:CDS
CGATGTTGGACTAGATGGAA+TGG 0.634701 3.1:+61193549 MS.gene98307:CDS
AATGGCCAAGCACATAGACC+TGG 0.655451 3.1:-61193475 None:intergenic
CATAGAGTAATGGACCGTTG+TGG 0.689245 3.1:-61193006 None:intergenic
CAGCTTCTTCATTACCACAA+CGG 0.692004 3.1:+61192992 MS.gene98307:CDS
GCAAATCACCAGCATAACCA+GGG 0.695085 3.1:-61193702 None:intergenic
GCACCTTTACCATAAACACC+AGG 0.705559 3.1:-61193676 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATCTCAGTCAATTTAATA+TGG + chr3.1:61193032-61193051 MS.gene98307:CDS 20.0%
!!! GATAATGTTTTTGTTTGTTA+AGG - chr3.1:61193269-61193288 None:intergenic 20.0%
! TCAGTCAATTTAATATGGTA+TGG + chr3.1:61193037-61193056 MS.gene98307:CDS 25.0%
! TTTGAGAAGAGATTGAAAAT+TGG - chr3.1:61192897-61192916 None:intergenic 25.0%
!! ATCAAGAATTTGCTTGTTTA+GGG - chr3.1:61193224-61193243 None:intergenic 25.0%
!! TTGATTATAGCTTTTTGTGA+TGG - chr3.1:61193073-61193092 None:intergenic 25.0%
AGATGGAATGGTGATAAATT+TGG + chr3.1:61193561-61193580 MS.gene98307:CDS 30.0%
CTTGATGAGAATTACTCATT+AGG + chr3.1:61193238-61193257 MS.gene98307:CDS 30.0%
GAAAGTAACCATTTCCAAAA+GGG - chr3.1:61193615-61193634 None:intergenic 30.0%
TGAAAGTAACCATTTCCAAA+AGG - chr3.1:61193616-61193635 None:intergenic 30.0%
TGCAAAATTCGCTTACATTT+GGG + chr3.1:61193429-61193448 MS.gene98307:CDS 30.0%
TTGCAAAATTCGCTTACATT+TGG + chr3.1:61193428-61193447 MS.gene98307:CDS 30.0%
! ATTATCCAATTAGCTTCCAA+AGG + chr3.1:61193283-61193302 MS.gene98307:CDS 30.0%
! CATCAAGAATTTGCTTGTTT+AGG - chr3.1:61193225-61193244 None:intergenic 30.0%
! GAGATTTTACCATAGAGTAA+TGG - chr3.1:61193019-61193038 None:intergenic 30.0%
! TTCTGTAGAACGTTTTTGTA+TGG + chr3.1:61193351-61193370 MS.gene98307:CDS 30.0%
! TTTGGAAATGGTTACTTTCA+AGG + chr3.1:61193616-61193635 MS.gene98307:CDS 30.0%
!!! ATTTTTTCTTTGAGGTGAGA+TGG - chr3.1:61193183-61193202 None:intergenic 30.0%
!!! CTGTGTAGATTTTTTCTTTG+AGG - chr3.1:61193191-61193210 None:intergenic 30.0%
AAATTCGCTTACATTTGGGT+TGG + chr3.1:61193433-61193452 MS.gene98307:CDS 35.0%
AATGGAATCCAGAAAAGGTA+AGG + chr3.1:61193405-61193424 MS.gene98307:CDS 35.0%
AGATGTTAGTACAACGTTGA+TGG - chr3.1:61193323-61193342 None:intergenic 35.0%
CCATTTCACCAACCAATTTA+CGG + chr3.1:61193493-61193512 MS.gene98307:CDS 35.0%
GTTGAGCAAGATAATGTAGA+AGG - chr3.1:61193808-61193827 None:intergenic 35.0%
TCTTCAATGGAATCCAGAAA+AGG + chr3.1:61193400-61193419 MS.gene98307:CDS 35.0%
TTCAATGCTCATGGTGATAA+TGG + chr3.1:61193751-61193770 MS.gene98307:CDS 35.0%
! TTTTGCAACCTTACCTTTTC+TGG - chr3.1:61193416-61193435 None:intergenic 35.0%
!! GAGATGGTAGTATTTTTCAG+TGG - chr3.1:61193167-61193186 None:intergenic 35.0%
!! TTGCTTGTTGATTCTACTAC+TGG + chr3.1:61193721-61193740 MS.gene98307:CDS 35.0%
!!! AATTTGGCTAGTCTTTTAGC+TGG + chr3.1:61193577-61193596 MS.gene98307:CDS 35.0%
ACACGGTTCTTCTTCTTCAA+TGG + chr3.1:61193387-61193406 MS.gene98307:CDS 40.0%
CAGCTTCTTCATTACCACAA+CGG + chr3.1:61192992-61193011 MS.gene98307:CDS 40.0%
GGAATCTTTGTGTTCACCTT+TGG - chr3.1:61193302-61193321 None:intergenic 40.0%
GGTAATGAAGAAGCTGAGAT+TGG - chr3.1:61192988-61193007 None:intergenic 40.0%
GTAGAAGGATCATACAAAGC+AGG - chr3.1:61193793-61193812 None:intergenic 40.0%
TCTAGTCCAACATCGTTGTT+TGG - chr3.1:61193544-61193563 None:intergenic 40.0%
TGAAGATGGTGAAGAAAGTG+AGG - chr3.1:61193107-61193126 None:intergenic 40.0%
TGTCCTGGTGTTTATGGTAA+AGG + chr3.1:61193673-61193692 MS.gene98307:CDS 40.0%
TTTGTGGACCGTAAATTGGT+TGG - chr3.1:61193504-61193523 None:intergenic 40.0%
! AACAACGATGTTGGACTAGA+TGG + chr3.1:61193544-61193563 MS.gene98307:CDS 40.0%
! GTTCACCTTTGGAAGCTAAT+TGG - chr3.1:61193291-61193310 None:intergenic 40.0%
!! AGTTTCTAGCAGCTGAAGAA+AGG - chr3.1:61192946-61192965 None:intergenic 40.0%
!! ATGCTCATGGTGATAATGGA+AGG + chr3.1:61193755-61193774 MS.gene98307:CDS 40.0%
!! CCGTAAATTGGTTGGTGAAA+TGG - chr3.1:61193496-61193515 None:intergenic 40.0%
!! GCAACAAACCCTTTTGGAAA+TGG + chr3.1:61193604-61193623 MS.gene98307:CDS 40.0%
!!! GGTTGGTTTGGTTTTGAAGA+TGG - chr3.1:61193121-61193140 None:intergenic 40.0%
AACCTTCAGTTTCAGCATGG+TGG + chr3.1:61193137-61193156 MS.gene98307:CDS 45.0%
AACTCAGAAACACAGTGTCC+AGG + chr3.1:61193457-61193476 MS.gene98307:CDS 45.0%
ACCAACCTTCAGTTTCAGCA+TGG + chr3.1:61193134-61193153 MS.gene98307:CDS 45.0%
ACCATGCTGAAACTGAAGGT+TGG - chr3.1:61193138-61193157 None:intergenic 45.0%
AGCAAATCACCAGCATAACC+AGG - chr3.1:61193706-61193725 None:intergenic 45.0%
CACAACGGTCCATTACTCTA+TGG + chr3.1:61193007-61193026 MS.gene98307:CDS 45.0%
GCAAATCACCAGCATAACCA+GGG - chr3.1:61193705-61193724 None:intergenic 45.0%
GCACCTTTACCATAAACACC+AGG - chr3.1:61193679-61193698 None:intergenic 45.0%
GTGCTTTGTGGACCGTAAAT+TGG - chr3.1:61193508-61193527 None:intergenic 45.0%
GTTGCACCAAACAACGATGT+TGG + chr3.1:61193535-61193554 MS.gene98307:CDS 45.0%
TCAGCTTGTCCTGGTGTTTA+TGG + chr3.1:61193667-61193686 MS.gene98307:CDS 45.0%
TTCCACCATGCTGAAACTGA+AGG - chr3.1:61193142-61193161 None:intergenic 45.0%
! CGATGTTGGACTAGATGGAA+TGG + chr3.1:61193549-61193568 MS.gene98307:CDS 45.0%
! CGTTTTTGTATGGACCGATG+TGG + chr3.1:61193361-61193380 MS.gene98307:CDS 45.0%
! GCTGAAACTGAAGGTTGGTT+TGG - chr3.1:61193133-61193152 None:intergenic 45.0%
! GGAACAGCAACAAACCCTTT+TGG + chr3.1:61193598-61193617 MS.gene98307:CDS 45.0%
! GGTGCTAGTTTCAATGCTCA+TGG + chr3.1:61193742-61193761 MS.gene98307:CDS 45.0%
!! CATAGAGTAATGGACCGTTG+TGG - chr3.1:61193009-61193028 None:intergenic 45.0%
!! TATGGTAAAGGTGCTTACCC+TGG + chr3.1:61193685-61193704 MS.gene98307:CDS 45.0%
!! TCGTTGTTTGGTGCAACCAA+TGG - chr3.1:61193532-61193551 None:intergenic 45.0%
AAGAACCGTGTGTGCCACAT+CGG - chr3.1:61193378-61193397 None:intergenic 50.0%
AATGGCCAAGCACATAGACC+TGG - chr3.1:61193478-61193497 None:intergenic 50.0%
AGTGTCCAGGTCTATGTGCT+TGG + chr3.1:61193470-61193489 MS.gene98307:CDS 50.0%
CAAGCTGAAGCAGCTTCAAG+AGG - chr3.1:61193655-61193674 None:intergenic 50.0%
GCAACCAATGGTGTGCTTTG+TGG - chr3.1:61193520-61193539 None:intergenic 50.0%
GCTTCAAGAGGAGCTTCAGA+AGG - chr3.1:61193643-61193662 None:intergenic 50.0%
ATGGACCGATGTGGCACACA+CGG + chr3.1:61193370-61193389 MS.gene98307:CDS 55.0%
CGGTCCACAAAGCACACCAT+TGG + chr3.1:61193513-61193532 MS.gene98307:CDS 55.0%
GAAGCTGCTTCAGCTTGTCC+TGG + chr3.1:61193658-61193677 MS.gene98307:CDS 55.0%
GGTGCTTACCCTGGTTATGC+TGG + chr3.1:61193694-61193713 MS.gene98307:CDS 55.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 61192887 61193837 61192887 ID=MS.gene98307
chr3.1 mRNA 61192887 61193837 61192887 ID=MS.gene98307.t1;Parent=MS.gene98307
chr3.1 exon 61192887 61193837 61192887 ID=MS.gene98307.t1.exon1;Parent=MS.gene98307.t1
chr3.1 CDS 61192887 61193837 61192887 ID=cds.MS.gene98307.t1;Parent=MS.gene98307.t1
Gene Sequence

>MS.gene98307

ATGGCTTCCAATTTTCAATCTCTTCTCAAATTTTTTCTTCTTATCTCAATTTTTTACCTTTCTTCAGCTGCTAGAAACTTGAACGAACTTGTTCAAGACCAATCTCAGCTTCTTCATTACCACAACGGTCCATTACTCTATGGTAAAATCTCAGTCAATTTAATATGGTATGGTAACTTTAAACCATCACAAAAAGCTATAATCAATGATTTTTTTACCTCACTTTCTTCACCATCTTCAAAACCAAACCAACCTTCAGTTTCAGCATGGTGGAAAACCACTGAAAAATACTACCATCTCACCTCAAAGAAAAAATCTACACAGCTTTCACTTTCCCTAAACAAGCAAATTCTTGATGAGAATTACTCATTAGGAAAATCCTTAACAAACAAAAACATTATCCAATTAGCTTCCAAAGGTGAACACAAAGATTCCATCAACGTTGTACTAACATCTGCTGATGTTTCTGTAGAACGTTTTTGTATGGACCGATGTGGCACACACGGTTCTTCTTCTTCAATGGAATCCAGAAAAGGTAAGGTTGCAAAATTCGCTTACATTTGGGTTGGTAACTCAGAAACACAGTGTCCAGGTCTATGTGCTTGGCCATTTCACCAACCAATTTACGGTCCACAAAGCACACCATTGGTTGCACCAAACAACGATGTTGGACTAGATGGAATGGTGATAAATTTGGCTAGTCTTTTAGCTGGAACAGCAACAAACCCTTTTGGAAATGGTTACTTTCAAGGTCCTTCTGAAGCTCCTCTTGAAGCTGCTTCAGCTTGTCCTGGTGTTTATGGTAAAGGTGCTTACCCTGGTTATGCTGGTGATTTGCTTGTTGATTCTACTACTGGTGCTAGTTTCAATGCTCATGGTGATAATGGAAGGAAGTATCTTCTTCCTGCTTTGTATGATCCTTCTACATTATCTTGCTCAACCCTTGTTTGA

Protein sequence

>MS.gene98307.t1

MASNFQSLLKFFLLISIFYLSSAARNLNELVQDQSQLLHYHNGPLLYGKISVNLIWYGNFKPSQKAIINDFFTSLSSPSSKPNQPSVSAWWKTTEKYYHLTSKKKSTQLSLSLNKQILDENYSLGKSLTNKNIIQLASKGEHKDSINVVLTSADVSVERFCMDRCGTHGSSSSMESRKGKVAKFAYIWVGNSETQCPGLCAWPFHQPIYGPQSTPLVAPNNDVGLDGMVINLASLLAGTATNPFGNGYFQGPSEAPLEAASACPGVYGKGAYPGYAGDLLVDSTTGASFNAHGDNGRKYLLPALYDPSTLSCSTLV