Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98379.t1 | KEH16865.1 | 99.3 | 285 | 2 | 0 | 1 | 285 | 14 | 298 | 6.90E-160 | 573.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98379.t1 | P93286 | 84.5 | 251 | 31 | 4 | 1 | 247 | 14 | 260 | 1.4e-108 | 394.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98379.t1 | A0A072TTM0 | 99.3 | 285 | 2 | 0 | 1 | 285 | 14 | 298 | 5.0e-160 | 573.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052239 | MS.gene98379 | 0.802595 | 5.21E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98379.t1 | MTR_0082s0260 | 99.298 | 285 | 2 | 0 | 1 | 285 | 14 | 298 | 0.0 | 579 |
| MS.gene98379.t1 | MTR_0024s0140 | 98.268 | 231 | 4 | 0 | 55 | 285 | 1 | 231 | 2.64e-167 | 462 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98379.t1 | ATMG00180 | 86.056 | 251 | 27 | 4 | 1 | 247 | 14 | 260 | 1.17e-124 | 363 |
| MS.gene98379.t1 | AT2G07815 | 92.063 | 63 | 5 | 0 | 1 | 63 | 186 | 248 | 1.94e-31 | 118 |
Find 0 sgRNAs with CRISPR-Local
Find 126 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAGATGAATATTTCACATTT+TGG | - | 6900:4818-4837 | MS.gene98379:CDS | 20.0% |
| ! | ATTATGTCTGGTAATAGAAT+AGG | + | 6900:4341-4360 | None:intergenic | 25.0% |
| ! | ATTCTATTACCAGACATAAT+TGG | - | 6900:4341-4360 | MS.gene98379:CDS | 25.0% |
| ! | TGTGAAATATTCATCTTCAT+TGG | + | 6900:4815-4834 | None:intergenic | 25.0% |
| ! | TTATGTCTGGTAATAGAATA+GGG | + | 6900:4340-4359 | None:intergenic | 25.0% |
| ! | TTCTATTACCAGACATAATT+GGG | - | 6900:4342-4361 | MS.gene98379:CDS | 25.0% |
| !! | TCATATTTAGAATCTTTCTG+CGG | - | 6900:4455-4474 | MS.gene98379:CDS | 25.0% |
| AATAGGAATTATAGTCCCAT+TGG | + | 6900:4219-4238 | None:intergenic | 30.0% | |
| AATGTAGATAGACCAAAAGA+GGG | + | 6900:4256-4275 | None:intergenic | 30.0% | |
| ACGAACCATTTCAATAGTAA+GGG | + | 6900:4164-4183 | None:intergenic | 30.0% | |
| ATAAGAAGGAAATGAAGAAC+GGG | + | 6900:4416-4435 | None:intergenic | 30.0% | |
| ATTATAGTCCCATTGGAAAA+AGG | + | 6900:4212-4231 | None:intergenic | 30.0% | |
| GATAAGAAGGAAATGAAGAA+CGG | + | 6900:4417-4436 | None:intergenic | 30.0% | |
| TTATAGTCCCATTGGAAAAA+GGG | + | 6900:4211-4230 | None:intergenic | 30.0% | |
| AACAACGAAATGACAAGATG+GGG | - | 6900:4600-4619 | MS.gene98379:CDS | 35.0% | |
| CAACAACGAAATGACAAGAT+GGG | - | 6900:4599-4618 | MS.gene98379:CDS | 35.0% | |
| GAATGTAGATAGACCAAAAG+AGG | + | 6900:4257-4276 | None:intergenic | 35.0% | |
| GACGAACCATTTCAATAGTA+AGG | + | 6900:4165-4184 | None:intergenic | 35.0% | |
| TATAGTCCCATTGGAAAAAG+GGG | + | 6900:4210-4229 | None:intergenic | 35.0% | |
| TTCCTTCTTATCGAATCCAA+GGG | - | 6900:4425-4444 | MS.gene98379:CDS | 35.0% | |
| TTCGACCCTTCTTTCTAAAT+GGG | + | 6900:4639-4658 | None:intergenic | 35.0% | |
| TTTCCTTCTTATCGAATCCA+AGG | - | 6900:4424-4443 | MS.gene98379:CDS | 35.0% | |
| ! | TCATCTTCATTGGAGCTTTT+TGG | + | 6900:4805-4824 | None:intergenic | 35.0% |
| ! | TTTTGGTCTATCTACATTCC+AGG | - | 6900:4258-4277 | MS.gene98379:CDS | 35.0% |
| AAATTCATACGCTCCATGGA+CGG | - | 6900:4281-4300 | MS.gene98379:CDS | 40.0% | |
| AGACCAAAAGAGGGAATGAA+GGG | + | 6900:4247-4266 | None:intergenic | 40.0% | |
| CAGCACCCTTACTATTGAAA+TGG | - | 6900:4156-4175 | MS.gene98379:CDS | 40.0% | |
| CATTTAGAAAGAAGGGTCGA+AGG | - | 6900:4638-4657 | MS.gene98379:CDS | 40.0% | |
| CTTCGACCCTTCTTTCTAAA+TGG | + | 6900:4640-4659 | None:intergenic | 40.0% | |
| GAAGGATACACCAAAGACTT+AGG | - | 6900:4555-4574 | MS.gene98379:CDS | 40.0% | |
| GCAACAACGAAATGACAAGA+TGG | - | 6900:4598-4617 | MS.gene98379:CDS | 40.0% | |
| GGGAATGAAGGGATAAGAAT+AGG | + | 6900:4236-4255 | None:intergenic | 40.0% | |
| TAAATATGAGAAGTCCCCCT+TGG | + | 6900:4444-4463 | None:intergenic | 40.0% | |
| TAGACCAAAAGAGGGAATGA+AGG | + | 6900:4248-4267 | None:intergenic | 40.0% | |
| TCCTTCTTATCGAATCCAAG+GGG | - | 6900:4426-4445 | MS.gene98379:CDS | 40.0% | |
| TCGACCCTTCTTTCTAAATG+GGG | + | 6900:4638-4657 | None:intergenic | 40.0% | |
| TGTCTGGTAATAGAATAGGG+CGG | + | 6900:4337-4356 | None:intergenic | 40.0% | |
| ! | AAAGAAGGGTCGAAGGTTTT+GGG | - | 6900:4645-4664 | MS.gene98379:CDS | 40.0% |
| ! | TATCCCTTCATTCCCTCTTT+TGG | - | 6900:4241-4260 | MS.gene98379:CDS | 40.0% |
| ! | TCTTCATTGGAGCTTTTTGG+TGG | + | 6900:4802-4821 | None:intergenic | 40.0% |
| AACGGGAACGAAACGAAATG+AGG | + | 6900:4399-4418 | None:intergenic | 45.0% | |
| AGACTTAGGCCTAACCCTAA+CGG | - | 6900:4569-4588 | MS.gene98379:CDS | 45.0% | |
| CAGGAAATTCATACGCTCCA+TGG | - | 6900:4277-4296 | MS.gene98379:CDS | 45.0% | |
| CATGGAGCGTATGAATTTCC+TGG | + | 6900:4279-4298 | None:intergenic | 45.0% | |
| CCTTCTTATCGAATCCAAGG+GGG | - | 6900:4427-4446 | MS.gene98379:CDS | 45.0% | |
| CGACCCTTCTTTCTAAATGG+GGG | + | 6900:4637-4656 | None:intergenic | 45.0% | |
| GAGCTTCTCCCAATTATGTC+TGG | + | 6900:4353-4372 | None:intergenic | 45.0% | |
| GCACCCCCATTTAGAAAGAA+GGG | - | 6900:4631-4650 | MS.gene98379:CDS | 45.0% | |
| GGAGTGTGCATCTGTATGTT+GGG | - | 6900:4839-4858 | MS.gene98379:CDS | 45.0% | |
| GTTGTTGCTCATTCCCGTTA+GGG | + | 6900:4586-4605 | None:intergenic | 45.0% | |
| TAGGGTTAGGCCTAAGTCTT+TGG | + | 6900:4568-4587 | None:intergenic | 45.0% | |
| TCTTCTCTTTACCACGCGAT+AGG | - | 6900:4507-4526 | MS.gene98379:CDS | 45.0% | |
| TGGAGTGTGCATCTGTATGT+TGG | - | 6900:4838-4857 | MS.gene98379:CDS | 45.0% | |
| ! | GAAAGAAGGGTCGAAGGTTT+TGG | - | 6900:4644-4663 | MS.gene98379:CDS | 45.0% |
| AGAGCTTCAAGCCCGATTTC+AGG | + | 6900:4734-4753 | None:intergenic | 50.0% | |
| AGCTCATCCGAAACTAGAGC+TGG | - | 6900:4368-4387 | MS.gene98379:CDS | 50.0% | |
| ATAGGTCAGCAAAGCGTGAG+CGG | - | 6900:4525-4544 | MS.gene98379:CDS | 50.0% | |
| CCCCCTTGGATTCGATAAGA+AGG | + | 6900:4430-4449 | None:intergenic | 50.0% | |
| CGGAGCAAAAAGTGGAGTCT+TGG | - | 6900:4301-4320 | MS.gene98379:CDS | 50.0% | |
| CGTTGTTGCTCATTCCCGTT+AGG | + | 6900:4587-4606 | None:intergenic | 50.0% | |
| GACTTAGGCCTAACCCTAAC+GGG | - | 6900:4570-4589 | MS.gene98379:CDS | 50.0% | |
| GGCACCCCCATTTAGAAAGA+AGG | - | 6900:4630-4649 | MS.gene98379:CDS | 50.0% | |
| GTCAGTAGAGATGTTCCCAC+AGG | - | 6900:4182-4201 | MS.gene98379:CDS | 50.0% | |
| GTGCCACCTGAACCTGAAAT+CGG | - | 6900:4719-4738 | MS.gene98379:CDS | 50.0% | |
| TCCATGGACGGAGCAAAAAG+TGG | - | 6900:4293-4312 | MS.gene98379:CDS | 50.0% | |
| TGCCACCTGAACCTGAAATC+GGG | - | 6900:4720-4739 | MS.gene98379:CDS | 50.0% | |
| TGCTCATTCCCGTTAGGGTT+AGG | + | 6900:4581-4600 | None:intergenic | 50.0% | |
| ! | AATGACAAGATGGGGTGCTC+CGG | - | 6900:4608-4627 | MS.gene98379:CDS | 50.0% |
| ! | ACAGGTGCCCCTTTTTCCAA+TGG | - | 6900:4200-4219 | MS.gene98379:CDS | 50.0% |
| ! | CAGGTGCCCCTTTTTCCAAT+GGG | - | 6900:4201-4220 | MS.gene98379:CDS | 50.0% |
| ! | GGCGTTTCCAGCTCTAGTTT+CGG | + | 6900:4378-4397 | None:intergenic | 50.0% |
| !! | ATTGGAAAAAGGGGCACCTG+TGG | + | 6900:4201-4220 | None:intergenic | 50.0% |
| !! | TCAAGCCCGATTTCAGGTTC+AGG | + | 6900:4728-4747 | None:intergenic | 50.0% |
| !! | TCTTTCTAAATGGGGGTGCC+CGG | + | 6900:4630-4649 | None:intergenic | 50.0% |
| !! | TTGGAAAAAGGGGCACCTGT+GGG | + | 6900:4200-4219 | None:intergenic | 50.0% |
| !!! | TCCACTTTTTGCTCCGTCCA+TGG | + | 6900:4297-4316 | None:intergenic | 50.0% |
| CCACGCCTCCTATTTGTTCG+GGG | - | 6900:4907-4926 | MS.gene98379:CDS | 55.0% | |
| CCCACGCCTCCTATTTGTTC+GGG | - | 6900:4906-4925 | MS.gene98379:CDS | 55.0% | |
| GACCAACAGCCATCAGCTGT+CGG | + | 6900:4772-4791 | None:intergenic | 55.0% | |
| GCCCACGCCTCCTATTTGTT+CGG | - | 6900:4905-4924 | MS.gene98379:CDS | 55.0% | |
| TAGGTCAGCAAAGCGTGAGC+GGG | - | 6900:4526-4545 | MS.gene98379:CDS | 55.0% | |
| TCAACTGAGCATCTCAGCGG+CGG | + | 6900:4972-4991 | None:intergenic | 55.0% | |
| TGTTCTTCTGTCGTGCGACC+CGG | - | 6900:4862-4881 | MS.gene98379:CDS | 55.0% | |
| ! | ATGACAAGATGGGGTGCTCC+GGG | - | 6900:4609-4628 | MS.gene98379:CDS | 55.0% |
| ! | CCCGAACAAATAGGAGGCGT+GGG | + | 6900:4909-4928 | None:intergenic | 55.0% |
| ! | CGTGAACTCTGACTCGATCC+GGG | - | 6900:4937-4956 | MS.gene98379:CDS | 55.0% |
| ! | GCGTGAACTCTGACTCGATC+CGG | - | 6900:4936-4955 | MS.gene98379:CDS | 55.0% |
| !! | AGCCCGATTTCAGGTTCAGG+TGG | + | 6900:4725-4744 | None:intergenic | 55.0% |
| ACAAGCACCACCCGACGAAG+GGG | + | 6900:4690-4709 | None:intergenic | 60.0% | |
| ACACAAGCACCACCCGACGA+AGG | + | 6900:4692-4711 | None:intergenic | 60.0% | |
| ACAGGTCGCACATAAGCCGC+CGG | + | 6900:4884-4903 | None:intergenic | 60.0% | |
| ACCAACGAGCCGACAGCTGA+TGG | - | 6900:4760-4779 | MS.gene98379:CDS | 60.0% | |
| AGCCGACAGCTGATGGCTGT+TGG | - | 6900:4767-4786 | MS.gene98379:CDS | 60.0% | |
| CAACTGAGCATCTCAGCGGC+GGG | + | 6900:4971-4990 | None:intergenic | 60.0% | |
| CACAAGCACCACCCGACGAA+GGG | + | 6900:4691-4710 | None:intergenic | 60.0% | |
| CAGCGGCGGGATTGAATACC+CGG | + | 6900:4958-4977 | None:intergenic | 60.0% | |
| CCCCGAACAAATAGGAGGCG+TGG | + | 6900:4910-4929 | None:intergenic | 60.0% | |
| CGCCTCCTATTTGTTCGGGG+CGG | - | 6900:4910-4929 | MS.gene98379:CDS | 60.0% | |
| CGCTTTGCTGACCTATCGCG+TGG | + | 6900:4521-4540 | None:intergenic | 60.0% | |
| GCCTCCTATTTGTTCGGGGC+GGG | - | 6900:4911-4930 | MS.gene98379:CDS | 60.0% | |
| TCCTATTTGTTCGGGGCGGG+CGG | - | 6900:4914-4933 | MS.gene98379:CDS | 60.0% | |
| TCGTCGGGTGGTGCTTGTGT+GGG | - | 6900:4692-4711 | MS.gene98379:CDS | 60.0% | |
| TTCGTCGGGTGGTGCTTGTG+TGG | - | 6900:4691-4710 | MS.gene98379:CDS | 60.0% | |
| ! | AAATAGGAGGCGTGGGCCAC+AGG | + | 6900:4902-4921 | None:intergenic | 60.0% |
| !! | CAGCTGTCGGCTCGTTGGTA+AGG | + | 6900:4759-4778 | None:intergenic | 60.0% |
| AAGCACCACCCGACGAAGGG+GGG | + | 6900:4688-4707 | None:intergenic | 65.0% | |
| CAAGCACCACCCGACGAAGG+GGG | + | 6900:4689-4708 | None:intergenic | 65.0% | |
| CAGGTCGCACATAAGCCGCC+GGG | + | 6900:4883-4902 | None:intergenic | 65.0% | |
| CGTCGGGTGGTGCTTGTGTG+GGG | - | 6900:4693-4712 | MS.gene98379:CDS | 65.0% | |
| GCCCGCCCCGAACAAATAGG+AGG | + | 6900:4915-4934 | None:intergenic | 65.0% | |
| GGCGGCTTATGTGCGACCTG+TGG | - | 6900:4883-4902 | MS.gene98379:CDS | 65.0% | |
| GTCGGGTGGTGCTTGTGTGG+GGG | - | 6900:4694-4713 | MS.gene98379:CDS | 65.0% | |
| TCGGGTGGTGCTTGTGTGGG+GGG | - | 6900:4695-4714 | MS.gene98379:CDS | 65.0% | |
| TCTTCTGTCGTGCGACCCGG+CGG | - | 6900:4865-4884 | MS.gene98379:CDS | 65.0% | |
| TTTCCCCGTCCCCCCTTCGT+CGG | - | 6900:4676-4695 | MS.gene98379:CDS | 65.0% | |
| !! | GCCATCAGCTGTCGGCTCGT+TGG | + | 6900:4764-4783 | None:intergenic | 65.0% |
| ACCACCCGACGAAGGGGGGA+CGG | + | 6900:4684-4703 | None:intergenic | 70.0% | |
| CAGCAAAGCGTGAGCGGGCG+CGG | - | 6900:4531-4550 | MS.gene98379:CDS | 70.0% | |
| GCCGCCCGCCCCGAACAAAT+AGG | + | 6900:4918-4937 | None:intergenic | 70.0% | |
| GGGGGGACGGGGAAAGCTAC+AGG | + | 6900:4671-4690 | None:intergenic | 70.0% | |
| TTCCCCGTCCCCCCTTCGTC+GGG | - | 6900:4677-4696 | MS.gene98379:CDS | 70.0% | |
| !! | AAGGGTGCTGCCGTGCCACG+AGG | + | 6900:4146-4165 | None:intergenic | 70.0% |
| AGCGTGAGCGGGCGCGGAGA+AGG | - | 6900:4537-4556 | MS.gene98379:CDS | 75.0% | |
| CACCCGACGAAGGGGGGACG+GGG | + | 6900:4682-4701 | None:intergenic | 75.0% | |
| CCACCCGACGAAGGGGGGAC+GGG | + | 6900:4683-4702 | None:intergenic | 75.0% | |
| ! | CCCGTCCCCCCTTCGTCGGG+TGG | - | 6900:4680-4699 | MS.gene98379:CDS | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 6900 | gene | 4134 | 4991 | 4134 | ID=MS.gene98379 |
| 6900 | mRNA | 4134 | 4991 | 4134 | ID=MS.gene98379.t1;Parent=MS.gene98379 |
| 6900 | exon | 4134 | 4991 | 4134 | ID=MS.gene98379.t1.exon1;Parent=MS.gene98379.t1 |
| 6900 | CDS | 4134 | 4991 | 4134 | ID=cds.MS.gene98379.t1;Parent=MS.gene98379.t1 |
| Gene Sequence |
| Protein sequence |
