AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene052239


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene052239.t1 MTR_3g087830 98.160 163 3 0 1 163 558 720 1.72e-110 330
MS.gene052239.t1 MTR_8g091530 48.837 215 57 2 1 163 548 761 4.81e-48 166
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene052239.t1 AT4G39680 55.063 158 57 1 1 158 488 631 2.42e-34 127
MS.gene052239.t1 AT4G39680 55.063 158 57 1 1 158 488 631 2.42e-34 127

Find 63 sgRNAs with CRISPR-Local

Find 74 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATAGCAATGAGTCTTTATT+TGG 0.236331 3.1:+70681575 None:intergenic
CTTACCAATGGTGGCGGAGG+TGG 0.329485 3.1:+70681186 None:intergenic
TGCTGCGAAACTTGCGGCTC+AGG 0.367848 3.1:-70681016 MS.gene052239:CDS
GGTGGTGCGGGAGGAACTAT+TGG 0.370795 3.1:+70681270 None:intergenic
GGCGGAGGTGGGAGAGTGAC+TGG 0.377606 3.1:+70681198 None:intergenic
AAGAGCCGCCCACCATTTGT+AGG 0.386711 3.1:+70681375 None:intergenic
CACGGCATTTCGTGTTTCAA+TGG 0.387909 3.1:+70681413 None:intergenic
GGCTGTGAAGAGGGTGGTGC+GGG 0.392874 3.1:+70681258 None:intergenic
GGCCTTACCAATGGTGGCGG+AGG 0.397144 3.1:+70681183 None:intergenic
TGCTGACGAGGGGAAGCCTC+TGG 0.436452 3.1:+70681237 None:intergenic
ATAGGTGGGTCCACTTTCTC+AGG 0.440175 3.1:+70681117 None:intergenic
GAAGCCTCTGGCTGTGAAGA+GGG 0.449251 3.1:+70681249 None:intergenic
ACCACCCCTTCCTGAGAAAG+TGG 0.451879 3.1:-70681127 MS.gene052239:CDS
TCAGGAAGGGGTGGTGGCGA+TGG 0.460747 3.1:+70681135 None:intergenic
GGCGTTCCCTAGCAACTGGT+GGG 0.468982 3.1:+70681160 None:intergenic
TGGGTCCACTTTCTCAGGAA+GGG 0.472429 3.1:+70681122 None:intergenic
AGGCTTCCCCTCGTCAGCAC+AGG 0.479056 3.1:-70681233 MS.gene052239:CDS
GAAGGGGTGGTGGCGATGGA+AGG 0.486557 3.1:+70681139 None:intergenic
CACCTCCGCCACCATTGGTA+AGG 0.487137 3.1:-70681185 MS.gene052239:CDS
GGAAGGCGTTCCCTAGCAAC+TGG 0.491793 3.1:+70681156 None:intergenic
CGTTCCCTAGCAACTGGTGG+GGG 0.497494 3.1:+70681162 None:intergenic
AGATCATCCAGAGTGACAAT+AGG 0.498410 3.1:+70681099 None:intergenic
TGTTTCAATGGCCTCTTCAG+TGG 0.506075 3.1:+70681425 None:intergenic
TTACCAATGGTGGCGGAGGT+GGG 0.506883 3.1:+70681187 None:intergenic
AGGCGTTCCCTAGCAACTGG+TGG 0.509948 3.1:+70681159 None:intergenic
ACTTTCTCAGGAAGGGGTGG+TGG 0.513958 3.1:+70681129 None:intergenic
TGTGAAGAGGGTGGTGCGGG+AGG 0.521080 3.1:+70681261 None:intergenic
GGGTCCACTTTCTCAGGAAG+GGG 0.524988 3.1:+70681123 None:intergenic
TCTCCCACCTCCGCCACCAT+TGG 0.525575 3.1:-70681190 MS.gene052239:CDS
TCCACTTTCTCAGGAAGGGG+TGG 0.525720 3.1:+70681126 None:intergenic
TTGCAATGGCCTACAAATGG+TGG 0.532540 3.1:-70681384 MS.gene052239:CDS
TGGCTGTGAAGAGGGTGGTG+CGG 0.535809 3.1:+70681257 None:intergenic
AATTTGCAATGGCCTACAAA+TGG 0.549283 3.1:-70681387 MS.gene052239:CDS
GTGGGTCCACTTTCTCAGGA+AGG 0.554750 3.1:+70681121 None:intergenic
GGACCCACCTATTGTCACTC+TGG 0.556935 3.1:-70681106 MS.gene052239:CDS
GGCTTCCCCTCGTCAGCACA+GGG 0.565114 3.1:-70681232 MS.gene052239:CDS
GCCTCTGGCTGTGAAGAGGG+TGG 0.565638 3.1:+70681252 None:intergenic
TCATCCAGAGTGACAATAGG+TGG 0.568024 3.1:+70681102 None:intergenic
TGTTGCTCCCTGTGCTGACG+AGG 0.570630 3.1:+70681225 None:intergenic
GCAGTACTCGTCCACTGAAG+AGG 0.574537 3.1:-70681436 MS.gene052239:intron
GCTGCGAAACTTGCGGCTCA+GGG 0.574547 3.1:-70681015 MS.gene052239:CDS
ACCACCCTCTTCACAGCCAG+AGG 0.575603 3.1:-70681253 MS.gene052239:CDS
GGGTAAGAGTACGAGGCAGT+AGG 0.576110 3.1:-70680995 None:intergenic
GGAAGCCTCTGGCTGTGAAG+AGG 0.578072 3.1:+70681248 None:intergenic
GCGTTCCCTAGCAACTGGTG+GGG 0.589857 3.1:+70681161 None:intergenic
ATTGGAGCACTGTTGACAGC+AGG 0.595299 3.1:+70681288 None:intergenic
TTGCTCCCTGTGCTGACGAG+GGG 0.600190 3.1:+70681227 None:intergenic
TGCAATGGCCTACAAATGGT+GGG 0.600370 3.1:-70681383 MS.gene052239:CDS
GTAAAACCACTGCAACTCCT+CGG 0.602190 3.1:-70681071 MS.gene052239:CDS
GCACTGTTGACAGCAGGAGT+AGG 0.605864 3.1:+70681294 None:intergenic
GTTGACAGCAGGAGTAGGAG+TGG 0.608389 3.1:+70681299 None:intergenic
ACAGCAGGAGTAGGAGTGGG+AGG 0.608778 3.1:+70681303 None:intergenic
CGGCTCAGGGTAAGAGTACG+AGG 0.610619 3.1:-70681002 MS.gene052239:CDS
TTGACAGCAGGAGTAGGAGT+GGG 0.613695 3.1:+70681300 None:intergenic
GTTGCTCCCTGTGCTGACGA+GGG 0.624628 3.1:+70681226 None:intergenic
ATGCCGTGTATAATTTGCAA+TGG 0.626656 3.1:-70681398 MS.gene052239:CDS
GCAAGTTGCTGCGAAACTTG+CGG 0.641051 3.1:-70681022 MS.gene052239:CDS
GCAACTTGCTCTTCTGACAA+AGG 0.648763 3.1:+70681036 None:intergenic
AAAGGTAAATAATAGATCCG+AGG 0.649093 3.1:+70681054 None:intergenic
AGGCCATTGCAAATTATACA+CGG 0.662782 3.1:+70681395 None:intergenic
CATCCAGAGTGACAATAGGT+GGG 0.665205 3.1:+70681103 None:intergenic
ATAGATCCGAGGAGTTGCAG+TGG 0.681963 3.1:+70681065 None:intergenic
AATGGCCTACAAATGGTGGG+CGG 0.733981 3.1:-70681380 MS.gene052239:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAGTAATCTTCTATTTTTTT+TGG - chr3.1:70681113-70681132 MS.gene052239:CDS 15.0%
!! ATAAATGTAATAGCATACAT+TGG + chr3.1:70681075-70681094 None:intergenic 20.0%
AAAGGTAAATAATAGATCCG+AGG + chr3.1:70681525-70681544 None:intergenic 30.0%
CATAGCAATGAGTCTTTATT+TGG + chr3.1:70681004-70681023 None:intergenic 30.0%
! AACTTCATTTTCACTTCTTC+AGG + chr3.1:70681246-70681265 None:intergenic 30.0%
AATTTGCAATGGCCTACAAA+TGG - chr3.1:70681189-70681208 MS.gene052239:CDS 35.0%
ACATTGGAATTCACAGTAAC+AGG + chr3.1:70681059-70681078 None:intergenic 35.0%
AGGCCATTGCAAATTATACA+CGG + chr3.1:70681184-70681203 None:intergenic 35.0%
! ATGCCGTGTATAATTTGCAA+TGG - chr3.1:70681178-70681197 MS.gene052239:CDS 35.0%
AGATCATCCAGAGTGACAAT+AGG + chr3.1:70681480-70681499 None:intergenic 40.0%
GGAATTCACAGTAACAGGAA+GGG + chr3.1:70681054-70681073 None:intergenic 40.0%
TGGAATTCACAGTAACAGGA+AGG + chr3.1:70681055-70681074 None:intergenic 40.0%
CACGGCATTTCGTGTTTCAA+TGG + chr3.1:70681166-70681185 None:intergenic 45.0%
CATCCAGAGTGACAATAGGT+GGG + chr3.1:70681476-70681495 None:intergenic 45.0%
GTAAAACCACTGCAACTCCT+CGG - chr3.1:70681505-70681524 MS.gene052239:intron 45.0%
TCATCCAGAGTGACAATAGG+TGG + chr3.1:70681477-70681496 None:intergenic 45.0%
TGCAATGGCCTACAAATGGT+GGG - chr3.1:70681193-70681212 MS.gene052239:CDS 45.0%
TGTTTCAATGGCCTCTTCAG+TGG + chr3.1:70681154-70681173 None:intergenic 45.0%
TTGCAATGGCCTACAAATGG+TGG - chr3.1:70681192-70681211 MS.gene052239:CDS 45.0%
! GCAACTTGCTCTTCTGACAA+AGG + chr3.1:70681543-70681562 None:intergenic 45.0%
!! GAGGAGTTGCAGTGGTTTTA+CGG + chr3.1:70681506-70681525 None:intergenic 45.0%
ATAGATCCGAGGAGTTGCAG+TGG + chr3.1:70681514-70681533 None:intergenic 50.0%
ATAGGTGGGTCCACTTTCTC+AGG + chr3.1:70681462-70681481 None:intergenic 50.0%
GCAAGTTGCTGCGAAACTTG+CGG - chr3.1:70681554-70681573 MS.gene052239:intron 50.0%
TGGGTCCACTTTCTCAGGAA+GGG + chr3.1:70681457-70681476 None:intergenic 50.0%
TTGACAGCAGGAGTAGGAGT+GGG + chr3.1:70681279-70681298 None:intergenic 50.0%
! AATGGCCTACAAATGGTGGG+CGG - chr3.1:70681196-70681215 MS.gene052239:CDS 50.0%
!! ATTGGAGCACTGTTGACAGC+AGG + chr3.1:70681291-70681310 None:intergenic 50.0%
AAGAGCCGCCCACCATTTGT+AGG + chr3.1:70681204-70681223 None:intergenic 55.0%
ACCACCCCTTCCTGAGAAAG+TGG - chr3.1:70681449-70681468 MS.gene052239:intron 55.0%
ACTTTCTCAGGAAGGGGTGG+TGG + chr3.1:70681450-70681469 None:intergenic 55.0%
GAAGCCTCTGGCTGTGAAGA+GGG + chr3.1:70681330-70681349 None:intergenic 55.0%
GCACTGTTGACAGCAGGAGT+AGG + chr3.1:70681285-70681304 None:intergenic 55.0%
GCAGTACTCGTCCACTGAAG+AGG - chr3.1:70681140-70681159 MS.gene052239:CDS 55.0%
GGACCCACCTATTGTCACTC+TGG - chr3.1:70681470-70681489 MS.gene052239:intron 55.0%
GGGTCCACTTTCTCAGGAAG+GGG + chr3.1:70681456-70681475 None:intergenic 55.0%
GTGGGTCCACTTTCTCAGGA+AGG + chr3.1:70681458-70681477 None:intergenic 55.0%
GTTGACAGCAGGAGTAGGAG+TGG + chr3.1:70681280-70681299 None:intergenic 55.0%
TCCACTTTCTCAGGAAGGGG+TGG + chr3.1:70681453-70681472 None:intergenic 55.0%
!! TTACCAATGGTGGCGGAGGT+GGG + chr3.1:70681392-70681411 None:intergenic 55.0%
AAGGCCCCCACCAGTTGCTA+GGG - chr3.1:70681410-70681429 MS.gene052239:CDS 60.0%
ACAGCAGGAGTAGGAGTGGG+AGG + chr3.1:70681276-70681295 None:intergenic 60.0%
ACCACCCTCTTCACAGCCAG+AGG - chr3.1:70681323-70681342 MS.gene052239:CDS 60.0%
ACTGGTGGGGGCCTTACCAA+TGG + chr3.1:70681405-70681424 None:intergenic 60.0%
AGGCGTTCCCTAGCAACTGG+TGG + chr3.1:70681420-70681439 None:intergenic 60.0%
CACCTCCGCCACCATTGGTA+AGG - chr3.1:70681391-70681410 MS.gene052239:CDS 60.0%
CGTTCCCTAGCAACTGGTGG+GGG + chr3.1:70681417-70681436 None:intergenic 60.0%
GCGTTCCCTAGCAACTGGTG+GGG + chr3.1:70681418-70681437 None:intergenic 60.0%
GCTGCGAAACTTGCGGCTCA+GGG - chr3.1:70681561-70681580 MS.gene052239:intron 60.0%
GGAAGCCTCTGGCTGTGAAG+AGG + chr3.1:70681331-70681350 None:intergenic 60.0%
GGCGTTCCCTAGCAACTGGT+GGG + chr3.1:70681419-70681438 None:intergenic 60.0%
GGTGGTGCGGGAGGAACTAT+TGG + chr3.1:70681309-70681328 None:intergenic 60.0%
TAAGGCCCCCACCAGTTGCT+AGG - chr3.1:70681409-70681428 MS.gene052239:CDS 60.0%
TGCTGCGAAACTTGCGGCTC+AGG - chr3.1:70681560-70681579 MS.gene052239:intron 60.0%
TGGCTGTGAAGAGGGTGGTG+CGG + chr3.1:70681322-70681341 None:intergenic 60.0%
! CGGCTCAGGGTAAGAGTACG+AGG - chr3.1:70681574-70681593 MS.gene052239:CDS 60.0%
! GTTGCTCCCTGTGCTGACGA+GGG + chr3.1:70681353-70681372 None:intergenic 60.0%
! TGTTGCTCCCTGTGCTGACG+AGG + chr3.1:70681354-70681373 None:intergenic 60.0%
! TTGCTCCCTGTGCTGACGAG+GGG + chr3.1:70681352-70681371 None:intergenic 60.0%
!! CTTACCAATGGTGGCGGAGG+TGG + chr3.1:70681393-70681412 None:intergenic 60.0%
!! GGAAGGCGTTCCCTAGCAAC+TGG + chr3.1:70681423-70681442 None:intergenic 60.0%
AGGCTTCCCCTCGTCAGCAC+AGG - chr3.1:70681343-70681362 MS.gene052239:CDS 65.0%
GCCTCTGGCTGTGAAGAGGG+TGG + chr3.1:70681327-70681346 None:intergenic 65.0%
GGCTGTGAAGAGGGTGGTGC+GGG + chr3.1:70681321-70681340 None:intergenic 65.0%
GGCTTCCCCTCGTCAGCACA+GGG - chr3.1:70681344-70681363 MS.gene052239:CDS 65.0%
GGGGGCCTTACCAATGGTGG+CGG + chr3.1:70681399-70681418 None:intergenic 65.0%
GGTGGGGGCCTTACCAATGG+TGG + chr3.1:70681402-70681421 None:intergenic 65.0%
TCTCCCACCTCCGCCACCAT+TGG - chr3.1:70681386-70681405 MS.gene052239:CDS 65.0%
TGCTGACGAGGGGAAGCCTC+TGG + chr3.1:70681342-70681361 None:intergenic 65.0%
TGTGAAGAGGGTGGTGCGGG+AGG + chr3.1:70681318-70681337 None:intergenic 65.0%
! GAAGGGGTGGTGGCGATGGA+AGG + chr3.1:70681440-70681459 None:intergenic 65.0%
! TCAGGAAGGGGTGGTGGCGA+TGG + chr3.1:70681444-70681463 None:intergenic 65.0%
!! GGCCTTACCAATGGTGGCGG+AGG + chr3.1:70681396-70681415 None:intergenic 65.0%
GGCGGAGGTGGGAGAGTGAC+TGG + chr3.1:70681381-70681400 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr3.1 gene 70680996 70681602 70680996 ID=MS.gene052239
chr3.1 mRNA 70680996 70681602 70680996 ID=MS.gene052239.t1;Parent=MS.gene052239
chr3.1 exon 70681570 70681602 70681570 ID=MS.gene052239.t1.exon1;Parent=MS.gene052239.t1
chr3.1 CDS 70681570 70681602 70681570 ID=cds.MS.gene052239.t1;Parent=MS.gene052239.t1
chr3.1 exon 70680996 70681454 70680996 ID=MS.gene052239.t1.exon2;Parent=MS.gene052239.t1
chr3.1 CDS 70680996 70681454 70680996 ID=cds.MS.gene052239.t1;Parent=MS.gene052239.t1
Gene Sequence

>MS.gene052239

ATGGACCAAATAAAGACTCATTGCTATGTTACTGTAAGTCAGTTCTTATCTCACTCCCTTCCTGTTACTGTGAATTCCAATGTATGCTATTACATTTATCATATGATTCTAACTTCTAAGTAATCTTCTATTTTTTTTGGTGATGCAGTACTCGTCCACTGAAGAGGCCATTGAAACACGAAATGCCGTGTATAATTTGCAATGGCCTACAAATGGTGGGCGGCTCTTAATTGCTGAGTTTGTTGATCCTGAAGAAGTGAAAATGAAGTTAGAAGCTCCTCCCACTCCTACTCCTGCTGTCAACAGTGCTCCAATAGTTCCTCCCGCACCACCCTCTTCACAGCCAGAGGCTTCCCCTCGTCAGCACAGGGAGCAACATCTTCCAGTCACTCTCCCACCTCCGCCACCATTGGTAAGGCCCCCACCAGTTGCTAGGGAACGCCTTCCATCGCCACCACCCCTTCCTGAGAAAGTGGACCCACCTATTGTCACTCTGGATGATCTCTTCCGTAAAACCACTGCAACTCCTCGGATCTATTATTTACCTTTGTCAGAAGAGCAAGTTGCTGCGAAACTTGCGGCTCAGGGTAAGAGTACGAGGCAGTAG

Protein sequence

>MS.gene052239.t1

MDQIKTHCYVTYSSTEEAIETRNAVYNLQWPTNGGRLLIAEFVDPEEVKMKLEAPPTPTPAVNSAPIVPPAPPSSQPEASPRQHREQHLPVTLPPPPPLVRPPPVARERLPSPPPLPEKVDPPIVTLDDLFRKTTATPRIYYLPLSEEQVAAKLAAQGKSTRQ