Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98393.t1 | XP_013463689.1 | 100 | 101 | 0 | 0 | 1 | 101 | 3 | 103 | 5.60E-48 | 199.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98393.t1 | B7FMQ0 | 100.0 | 101 | 0 | 0 | 1 | 101 | 3 | 103 | 4.1e-48 | 199.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene055162 | MS.gene98393 | 0.801384 | 9.24E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98393.t1 | MTR_2g046160 | 100.000 | 101 | 0 | 0 | 1 | 101 | 3 | 103 | 7.90e-70 | 203 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98393.t1 | AT5G57860 | 63.333 | 90 | 33 | 0 | 1 | 90 | 3 | 92 | 1.71e-39 | 127 |
| MS.gene98393.t1 | AT5G57860 | 63.333 | 90 | 33 | 0 | 1 | 90 | 3 | 92 | 1.71e-39 | 127 |
| MS.gene98393.t1 | AT5G57860 | 63.333 | 90 | 33 | 0 | 1 | 90 | 3 | 92 | 1.71e-39 | 127 |
| MS.gene98393.t1 | AT5G57860 | 57.143 | 63 | 27 | 0 | 1 | 63 | 3 | 65 | 1.45e-22 | 83.2 |
Find 24 sgRNAs with CRISPR-Local
Find 78 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGATAAAATTCTTGACATAA+AGG | 0.387433 | 2.4:+44867014 | MS.gene98393:CDS |
| TAAGACTCTGGCTGATCAAA+AGG | 0.457105 | 2.4:+44867119 | MS.gene98393:CDS |
| ATCAACCGTTGGTCCTTAGC+AGG | 0.459907 | 2.4:-44867063 | None:intergenic |
| TCCAGATGATAATGAGTTTG+AGG | 0.484717 | 2.4:+44868042 | MS.gene98393:intron |
| CGGTTGATTCTACCTGGTAC+AGG | 0.492432 | 2.4:+44867078 | MS.gene98393:CDS |
| ATTAGATGACTCTAAGACTC+TGG | 0.501981 | 2.4:+44867107 | MS.gene98393:CDS |
| CTTGTCTGATAGAAATCACT+TGG | 0.537926 | 2.4:-44868082 | None:intergenic |
| CCCAAGTTTACCTTTCCTCA+AGG | 0.539674 | 2.4:-44867383 | None:intergenic |
| TCTAATACTTCCCCTGTACC+AGG | 0.549770 | 2.4:-44867090 | None:intergenic |
| GCTTGTTGACAAACCTGCTA+AGG | 0.558954 | 2.4:+44867050 | MS.gene98393:CDS |
| GGTTGATTCTACCTGGTACA+GGG | 0.577903 | 2.4:+44867079 | MS.gene98393:CDS |
| AACATACTTCATCCGATGCA+AGG | 0.594573 | 2.4:+44866987 | MS.gene98393:CDS |
| CAAGTGATTTCTATCAGACA+AGG | 0.603115 | 2.4:+44868083 | MS.gene98393:CDS |
| CTGTTGTTGCACTGACCTTG+AGG | 0.605739 | 2.4:+44867368 | MS.gene98393:CDS |
| GACCAACGGTTGATTCTACC+TGG | 0.613786 | 2.4:+44867072 | MS.gene98393:CDS |
| CTATCAGACAAGGGATGCAG+AGG | 0.622837 | 2.4:+44868093 | MS.gene98393:CDS |
| AGATGATAATGAGTTTGAGG+AGG | 0.645303 | 2.4:+44868045 | MS.gene98393:intron |
| GTTGATTCTACCTGGTACAG+GGG | 0.650099 | 2.4:+44867080 | MS.gene98393:CDS |
| GGGATGCAGAGGGTGCCAGT+TGG | 0.653381 | 2.4:+44868104 | MS.gene98393:CDS |
| ACAAACCTGCTAAGGACCAA+CGG | 0.658865 | 2.4:+44867058 | MS.gene98393:CDS |
| TACCAGGTAGAATCAACCGT+TGG | 0.662250 | 2.4:-44867074 | None:intergenic |
| TATCAGACAAGGGATGCAGA+GGG | 0.682991 | 2.4:+44868094 | MS.gene98393:CDS |
| GTTGCACTGACCTTGAGGAA+AGG | 0.688680 | 2.4:+44867373 | MS.gene98393:CDS |
| AAGTGATTTCTATCAGACAA+GGG | 0.710731 | 2.4:+44868084 | MS.gene98393:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GAAATTTATGAATTTATATT+AGG | + | chr2.4:44867414-44867433 | MS.gene98393:intron | 10.0% |
| !! | TATAAATTCATAAATTTCTA+AGG | - | chr2.4:44867412-44867431 | None:intergenic | 10.0% |
| !!! | AAATTTAAAATTGTTGCATA+AGG | - | chr2.4:44867663-44867682 | None:intergenic | 15.0% |
| !!! | AATCTGAAATGTAAAAAAAA+TGG | - | chr2.4:44867338-44867357 | None:intergenic | 15.0% |
| !! | ATTAACCCATATTAAAGTTA+GGG | - | chr2.4:44867823-44867842 | None:intergenic | 20.0% |
| !! | TGATAAAATTCTTGACATAA+AGG | + | chr2.4:44867014-44867033 | MS.gene98393:CDS | 20.0% |
| !! | TTTGTTCAAGTGAATAAAAA+GGG | - | chr2.4:44867514-44867533 | None:intergenic | 20.0% |
| !!! | TTTTGTTCAAGTGAATAAAA+AGG | - | chr2.4:44867515-44867534 | None:intergenic | 20.0% |
| ! | AGATGATACAAAAATGAACA+AGG | - | chr2.4:44867547-44867566 | None:intergenic | 25.0% |
| ! | AGTTAGAAACTAAGAATCAA+TGG | - | chr2.4:44867249-44867268 | None:intergenic | 25.0% |
| ! | CAAACTAGTATGTTATTCAT+CGG | + | chr2.4:44868150-44868169 | MS.gene98393:intron | 25.0% |
| ! | CATTAACCCATATTAAAGTT+AGG | - | chr2.4:44867824-44867843 | None:intergenic | 25.0% |
| ! | TAAAATTGTTGCATAAGGAT+AGG | - | chr2.4:44867658-44867677 | None:intergenic | 25.0% |
| !!! | TGTTGTTTCTGAAAAAGATA+TGG | - | chr2.4:44867996-44868015 | None:intergenic | 25.0% |
| AAGTGATTTCTATCAGACAA+GGG | + | chr2.4:44868084-44868103 | MS.gene98393:CDS | 30.0% | |
| ACACGTAGTTTATTCACATT+GGG | + | chr2.4:44867471-44867490 | MS.gene98393:intron | 30.0% | |
| AGATGTAAACAAGTAAATGC+AGG | - | chr2.4:44867908-44867927 | None:intergenic | 30.0% | |
| AGGTTGTACATACAATCTAA+AGG | - | chr2.4:44867638-44867657 | None:intergenic | 30.0% | |
| CATGACCCTAACTTTAATAT+GGG | + | chr2.4:44867815-44867834 | MS.gene98393:intron | 30.0% | |
| CGATGAATAACATACTAGTT+TGG | - | chr2.4:44868152-44868171 | None:intergenic | 30.0% | |
| GAAGCAATCAGAATTATTTC+AGG | - | chr2.4:44867274-44867293 | None:intergenic | 30.0% | |
| GAATAAAGATAAGCATACTC+AGG | - | chr2.4:44867601-44867620 | None:intergenic | 30.0% | |
| TACACGTAGTTTATTCACAT+TGG | + | chr2.4:44867470-44867489 | MS.gene98393:intron | 30.0% | |
| TTGCTAGTTATACCCTTATA+TGG | - | chr2.4:44867774-44867793 | None:intergenic | 30.0% | |
| ! | ATACTGTCTGTTAATTTTCC+AGG | + | chr2.4:44868227-44868246 | MS.gene98393:intron | 30.0% |
| ! | TGAATAACATACTAGTTTGG+TGG | - | chr2.4:44868149-44868168 | None:intergenic | 30.0% |
| !! | ATTCCTCTAAAGGATCATTT+TGG | + | chr2.4:44867945-44867964 | MS.gene98393:intron | 30.0% |
| !!! | AAACACACTCTAACTTGTTT+TGG | + | chr2.4:44867443-44867462 | MS.gene98393:intron | 30.0% |
| AACACAGATAGAATCAAAGC+TGG | - | chr2.4:44867221-44867240 | None:intergenic | 35.0% | |
| AAGATATGGCAGTACACTAA+TGG | - | chr2.4:44867982-44868001 | None:intergenic | 35.0% | |
| ACCCTTATATGGTAGAAACA+GGG | - | chr2.4:44867763-44867782 | None:intergenic | 35.0% | |
| ACGTAGTTTATTCACATTGG+GGG | + | chr2.4:44867473-44867492 | MS.gene98393:intron | 35.0% | |
| CAAGTGATTTCTATCAGACA+AGG | + | chr2.4:44868083-44868102 | MS.gene98393:CDS | 35.0% | |
| CACGTAGTTTATTCACATTG+GGG | + | chr2.4:44867472-44867491 | MS.gene98393:intron | 35.0% | |
| CATCCAAAATGATCCTTTAG+AGG | - | chr2.4:44867951-44867970 | None:intergenic | 35.0% | |
| CTTGTCTGATAGAAATCACT+TGG | - | chr2.4:44868085-44868104 | None:intergenic | 35.0% | |
| GCATGACCCTAACTTTAATA+TGG | + | chr2.4:44867814-44867833 | MS.gene98393:intron | 35.0% | |
| TACCCTTATATGGTAGAAAC+AGG | - | chr2.4:44867764-44867783 | None:intergenic | 35.0% | |
| TATAACTAGCAATGCCCTTA+GGG | + | chr2.4:44867782-44867801 | MS.gene98393:intron | 35.0% | |
| TCCTCAAACTCATTATCATC+TGG | - | chr2.4:44868046-44868065 | None:intergenic | 35.0% | |
| TGACAACATCATTCCTCTAA+AGG | + | chr2.4:44867935-44867954 | MS.gene98393:intron | 35.0% | |
| ! | AGATGATAATGAGTTTGAGG+AGG | + | chr2.4:44868045-44868064 | MS.gene98393:intron | 35.0% |
| ! | TCCAGATGATAATGAGTTTG+AGG | + | chr2.4:44868042-44868061 | MS.gene98393:intron | 35.0% |
| ! | TTTTATCAGATGCCTTGCAT+CGG | - | chr2.4:44867002-44867021 | None:intergenic | 35.0% |
| !! | ATTAGATGACTCTAAGACTC+TGG | + | chr2.4:44867107-44867126 | MS.gene98393:CDS | 35.0% |
| !! | TCCCTGTTTCTACCATATAA+GGG | + | chr2.4:44867759-44867778 | MS.gene98393:intron | 35.0% |
| !! | TTCCCTGTTTCTACCATATA+AGG | + | chr2.4:44867758-44867777 | MS.gene98393:intron | 35.0% |
| AACATACTTCATCCGATGCA+AGG | + | chr2.4:44866987-44867006 | MS.gene98393:CDS | 40.0% | |
| ACCTTGAGGAAAGGTAAACT+TGG | + | chr2.4:44867382-44867401 | MS.gene98393:intron | 40.0% | |
| CAGTATGTCAGCTCTTATCA+TGG | - | chr2.4:44868213-44868232 | None:intergenic | 40.0% | |
| CATGCTAACAAGTACCCTAA+GGG | - | chr2.4:44867799-44867818 | None:intergenic | 40.0% | |
| CCTTGAGGAAAGGTAAACTT+GGG | + | chr2.4:44867383-44867402 | MS.gene98393:intron | 40.0% | |
| CGTAGTTTATTCACATTGGG+GGG | + | chr2.4:44867474-44867493 | MS.gene98393:intron | 40.0% | |
| CTTGAGGAAAGGTAAACTTG+GGG | + | chr2.4:44867384-44867403 | MS.gene98393:intron | 40.0% | |
| GTATAACTAGCAATGCCCTT+AGG | + | chr2.4:44867781-44867800 | MS.gene98393:intron | 40.0% | |
| TAAGACTCTGGCTGATCAAA+AGG | + | chr2.4:44867119-44867138 | MS.gene98393:CDS | 40.0% | |
| TATTCACATTGGGGGGATAT+TGG | + | chr2.4:44867481-44867500 | MS.gene98393:intron | 40.0% | |
| TCATGCTAACAAGTACCCTA+AGG | - | chr2.4:44867800-44867819 | None:intergenic | 40.0% | |
| ! | CATACTAGTTTGGTGGAGTA+TGG | - | chr2.4:44868142-44868161 | None:intergenic | 40.0% |
| ! | TGGCTTTACCAATTACCAAC+TGG | - | chr2.4:44868122-44868141 | None:intergenic | 40.0% |
| ACAAACCTGCTAAGGACCAA+CGG | + | chr2.4:44867058-44867077 | MS.gene98393:CDS | 45.0% | |
| CCCAAGTTTACCTTTCCTCA+AGG | - | chr2.4:44867386-44867405 | None:intergenic | 45.0% | |
| TACCAGGTAGAATCAACCGT+TGG | - | chr2.4:44867077-44867096 | None:intergenic | 45.0% | |
| TATCAGACAAGGGATGCAGA+GGG | + | chr2.4:44868094-44868113 | MS.gene98393:CDS | 45.0% | |
| TCTAATACTTCCCCTGTACC+AGG | - | chr2.4:44867093-44867112 | None:intergenic | 45.0% | |
| ! | GCTTGTTGACAAACCTGCTA+AGG | + | chr2.4:44867050-44867069 | MS.gene98393:CDS | 45.0% |
| ! | GGTTGATTCTACCTGGTACA+GGG | + | chr2.4:44867079-44867098 | MS.gene98393:CDS | 45.0% |
| ! | GTTGATTCTACCTGGTACAG+GGG | + | chr2.4:44867080-44867099 | MS.gene98393:CDS | 45.0% |
| !! | AAATTTATGAATTTATATTA+GGG | + | chr2.4:44867415-44867434 | MS.gene98393:intron | 5.0% |
| ATCAACCGTTGGTCCTTAGC+AGG | - | chr2.4:44867066-44867085 | None:intergenic | 50.0% | |
| CTATCAGACAAGGGATGCAG+AGG | + | chr2.4:44868093-44868112 | MS.gene98393:CDS | 50.0% | |
| GGTAGAAACAGGGAAGTCGA+TGG | - | chr2.4:44867753-44867772 | None:intergenic | 50.0% | |
| ! | CGGTTGATTCTACCTGGTAC+AGG | + | chr2.4:44867078-44867097 | MS.gene98393:CDS | 50.0% |
| ! | CTGTTGTTGCACTGACCTTG+AGG | + | chr2.4:44867368-44867387 | MS.gene98393:CDS | 50.0% |
| ! | GACCAACGGTTGATTCTACC+TGG | + | chr2.4:44867072-44867091 | MS.gene98393:CDS | 50.0% |
| ! | GTTGCACTGACCTTGAGGAA+AGG | + | chr2.4:44867373-44867392 | MS.gene98393:CDS | 50.0% |
| !! | AGAGGGTGCCAGTTGGTAAT+TGG | + | chr2.4:44868111-44868130 | MS.gene98393:intron | 50.0% |
| !! | GGGATGCAGAGGGTGCCAGT+TGG | + | chr2.4:44868104-44868123 | MS.gene98393:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr2.4 | gene | 44866958 | 44868252 | 44866958 | ID=MS.gene98393 |
| chr2.4 | mRNA | 44866958 | 44868252 | 44866958 | ID=MS.gene98393.t1;Parent=MS.gene98393 |
| chr2.4 | exon | 44866958 | 44867140 | 44866958 | ID=MS.gene98393.t1.exon1;Parent=MS.gene98393.t1 |
| chr2.4 | CDS | 44866958 | 44867140 | 44866958 | ID=cds.MS.gene98393.t1;Parent=MS.gene98393.t1 |
| chr2.4 | exon | 44867355 | 44867394 | 44867355 | ID=MS.gene98393.t1.exon2;Parent=MS.gene98393.t1 |
| chr2.4 | CDS | 44867355 | 44867394 | 44867355 | ID=cds.MS.gene98393.t1;Parent=MS.gene98393.t1 |
| chr2.4 | exon | 44868047 | 44868125 | 44868047 | ID=MS.gene98393.t1.exon3;Parent=MS.gene98393.t1 |
| chr2.4 | CDS | 44868047 | 44868125 | 44868047 | ID=cds.MS.gene98393.t1;Parent=MS.gene98393.t1 |
| chr2.4 | exon | 44868249 | 44868252 | 44868249 | ID=MS.gene98393.t1.exon4;Parent=MS.gene98393.t1 |
| chr2.4 | CDS | 44868249 | 44868252 | 44868249 | ID=cds.MS.gene98393.t1;Parent=MS.gene98393.t1 |
| Gene Sequence |
| Protein sequence |
