Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98428.t1 | KEH16787.1 | 96 | 174 | 7 | 0 | 31 | 204 | 153 | 326 | 8.80E-88 | 333.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98428.t1 | A0A072THA4 | 96.0 | 174 | 7 | 0 | 31 | 204 | 153 | 326 | 6.4e-88 | 333.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene055841 | MS.gene98428 | -0.812785 | 3.56E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98428.t1 | MTR_0094s0120 | 94.886 | 176 | 9 | 0 | 31 | 206 | 153 | 328 | 3.44e-119 | 343 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 0 sgRNAs with CRISPR-Local
Find 95 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | CATTTTTTTCTTCCTTATTT+TGG | + | 57115:13498-13517 | MS.gene98428:CDS | 20.0% |
| ! | TCAATAGAAATGCCAAAATA+AGG | - | 57115:13513-13532 | MS.gene98428:intergenic | 25.0% |
| !! | AAAAATGGATTTGTATTGTC+GGG | - | 57115:14055-14074 | MS.gene98428:intergenic | 25.0% |
| !! | CAAAAATGGATTTGTATTGT+CGG | - | 57115:14056-14075 | MS.gene98428:intergenic | 25.0% |
| AGACTTCGTTGCAAATAAAT+TGG | + | 57115:13900-13919 | MS.gene98428:CDS | 30.0% | |
| CCACTTTACTTTCAAAAGAT+CGG | - | 57115:13548-13567 | MS.gene98428:intergenic | 30.0% | |
| ! | AAAATGGATTTGTATTGTCG+GGG | - | 57115:14054-14073 | MS.gene98428:intergenic | 30.0% |
| ! | CGACAATACAAATCCATTTT+TGG | + | 57115:14054-14073 | MS.gene98428:CDS | 30.0% |
| CAAAAGAAATACGATTGCCA+AGG | - | 57115:13632-13651 | MS.gene98428:intergenic | 35.0% | |
| TGCAAATAAATTGGAAGACC+CGG | + | 57115:13909-13928 | MS.gene98428:CDS | 35.0% | |
| ! | AAATGGATTTGTATTGTCGG+GGG | - | 57115:14053-14072 | MS.gene98428:intergenic | 35.0% |
| !! | CCGATCTTTTGAAAGTAAAG+TGG | + | 57115:13545-13564 | MS.gene98428:CDS | 35.0% |
| AAGAAATACGATTGCCAAGG+CGG | - | 57115:13629-13648 | MS.gene98428:intergenic | 40.0% | |
| CAAATAAATTGGAAGACCCG+GGG | + | 57115:13911-13930 | MS.gene98428:CDS | 40.0% | |
| CAGAAGGATACATCCAAAGA+GGG | - | 57115:13681-13700 | MS.gene98428:intergenic | 40.0% | |
| CTCTAAACCTCCTCTTTACT+CGG | + | 57115:13584-13603 | MS.gene98428:CDS | 40.0% | |
| CTCTTTGGATGTATCCTTCT+GGG | + | 57115:13680-13699 | MS.gene98428:CDS | 40.0% | |
| GCAAATAAATTGGAAGACCC+GGG | + | 57115:13910-13929 | MS.gene98428:CDS | 40.0% | |
| GTTTAGAGACCTGCTGAAAA+AGG | - | 57115:13572-13591 | MS.gene98428:intergenic | 40.0% | |
| TCTTTGGATGTATCCTTCTG+GGG | + | 57115:13681-13700 | MS.gene98428:CDS | 40.0% | |
| !!! | AATCCATTTTTGGCTTCTCC+CGG | + | 57115:14064-14083 | MS.gene98428:CDS | 40.0% |
| !!! | TAAAGTGGACCTTTTTCAGC+AGG | + | 57115:13560-13579 | MS.gene98428:CDS | 40.0% |
| CATCCAAAGAGGGATTTCCT+CGG | - | 57115:13671-13690 | MS.gene98428:intergenic | 45.0% | |
| CCAGAAGGATACATCCAAAG+AGG | - | 57115:13682-13701 | MS.gene98428:intergenic | 45.0% | |
| CCTCTTTGGATGTATCCTTC+TGG | + | 57115:13679-13698 | MS.gene98428:CDS | 45.0% | |
| CGTAGCGCTTGCTCATAAAT+TGG | - | 57115:13949-13968 | MS.gene98428:intergenic | 45.0% | |
| TCTCGAGTTCATACCATACC+AGG | - | 57115:13972-13991 | MS.gene98428:intergenic | 45.0% | |
| TGCTCATAAATTGGACCCTG+CGG | - | 57115:13940-13959 | MS.gene98428:intergenic | 45.0% | |
| TGGTATGAACTCGAGATCGA+TGG | + | 57115:13976-13995 | MS.gene98428:CDS | 45.0% | |
| !!! | ATCCATTTTTGGCTTCTCCC+GGG | + | 57115:14065-14084 | MS.gene98428:CDS | 45.0% |
| AACCTCCTCTTTACTCGGCT+CGG | + | 57115:13589-13608 | MS.gene98428:CDS | 50.0% | |
| AGCTGAAGGAGGGCTATGTA+TGG | - | 57115:13844-13863 | MS.gene98428:intergenic | 50.0% | |
| ATAGCCCTCCTTCAGCTAGA+GGG | + | 57115:13847-13866 | MS.gene98428:CDS | 50.0% | |
| CGCTCCTATCCAAAATGGGT+CGG | + | 57115:13771-13790 | MS.gene98428:CDS | 50.0% | |
| GAGGGCTATGTATGGTTTCG+CGG | - | 57115:13836-13855 | MS.gene98428:intergenic | 50.0% | |
| GATCGATGGCAGTACTAACC+AGG | + | 57115:13990-14009 | MS.gene98428:CDS | 50.0% | |
| TATCCAAAATGGGTCGGCGT+CGG | + | 57115:13777-13796 | MS.gene98428:CDS | 50.0% | |
| ! | CGACCCATTTTGGATAGGAG+CGG | - | 57115:13773-13792 | MS.gene98428:intergenic | 50.0% |
| !!! | TCCATTTTTGGCTTCTCCCG+GGG | + | 57115:14066-14085 | MS.gene98428:CDS | 50.0% |
| AATACGATTGCCAAGGCGGC+CGG | - | 57115:13625-13644 | MS.gene98428:intergenic | 55.0% | |
| ACCTCCTCTTTACTCGGCTC+GGG | + | 57115:13590-13609 | MS.gene98428:CDS | 55.0% | |
| CATAGCCCTCCTTCAGCTAG+AGG | + | 57115:13846-13865 | MS.gene98428:CDS | 55.0% | |
| CCGCCGCTCCTATCCAAAAT+GGG | + | 57115:13767-13786 | MS.gene98428:CDS | 55.0% | |
| GCTATGTATGGTTTCGCGGC+CGG | - | 57115:13832-13851 | MS.gene98428:intergenic | 55.0% | |
| GTGCCGAGGAAATCCCTCTT+TGG | + | 57115:13665-13684 | MS.gene98428:CDS | 55.0% | |
| TAGCCCTCCTTCAGCTAGAG+GGG | + | 57115:13848-13867 | MS.gene98428:CDS | 55.0% | |
| TTATGAGCAAGCGCTACGCC+TGG | + | 57115:13951-13970 | MS.gene98428:CDS | 55.0% | |
| ! | CCATTTTGGATAGGAGCGGC+GGG | - | 57115:13769-13788 | MS.gene98428:intergenic | 55.0% |
| ! | CCCATTTTGGATAGGAGCGG+CGG | - | 57115:13770-13789 | MS.gene98428:intergenic | 55.0% |
| ! | GACGCCGACCCATTTTGGAT+AGG | - | 57115:13778-13797 | MS.gene98428:intergenic | 55.0% |
| !! | AGCGCTGGCTGGTTTCACTA+AGG | + | 57115:14022-14041 | MS.gene98428:CDS | 55.0% |
| !! | CTCTTTACTCGGCTCGGGTT+CGG | + | 57115:13595-13614 | MS.gene98428:CDS | 55.0% |
| !! | GCGCTGGCTGGTTTCACTAA+GGG | + | 57115:14023-14042 | MS.gene98428:CDS | 55.0% |
| !! | TATCCTTCTGGGGCTGATGC+TGG | + | 57115:13691-13710 | MS.gene98428:CDS | 55.0% |
| !! | TCTTTACTCGGCTCGGGTTC+GGG | + | 57115:13596-13615 | MS.gene98428:CDS | 55.0% |
| !!! | CATTTTTGGCTTCTCCCGGG+GGG | + | 57115:14068-14087 | MS.gene98428:CDS | 55.0% |
| !!! | CCATTTTTGGCTTCTCCCGG+GGG | + | 57115:14067-14086 | MS.gene98428:CDS | 55.0% |
| ACCAGGACGAACTTCAGCGC+TGG | + | 57115:14007-14026 | MS.gene98428:CDS | 60.0% | |
| ACCCGAGCCGAGTAAAGAGG+AGG | - | 57115:13594-13613 | MS.gene98428:intergenic | 60.0% | |
| AGCAAGCGCTACGCCTGGTA+TGG | + | 57115:13956-13975 | MS.gene98428:CDS | 60.0% | |
| AGCCCTCCTTCAGCTAGAGG+GGG | + | 57115:13849-13868 | MS.gene98428:CDS | 60.0% | |
| CCCCCGGGAGAAGCCAAAAA+TGG | - | 57115:14070-14089 | MS.gene98428:intergenic | 60.0% | |
| CCCGCCGCTCCTATCCAAAA+TGG | + | 57115:13766-13785 | MS.gene98428:CDS | 60.0% | |
| CGAACCCGAGCCGAGTAAAG+AGG | - | 57115:13597-13616 | MS.gene98428:intergenic | 60.0% | |
| CTCCCCCTCTAGCTGAAGGA+GGG | - | 57115:13854-13873 | MS.gene98428:intergenic | 60.0% | |
| GCGAGCACTATTCAGTGCCG+AGG | + | 57115:13651-13670 | MS.gene98428:CDS | 60.0% | |
| GGGAAGGCTGATGCTGCTCT+TGG | - | 57115:13735-13754 | MS.gene98428:intergenic | 60.0% | |
| TCCTTCAGCTAGAGGGGGAG+CGG | + | 57115:13854-13873 | MS.gene98428:CDS | 60.0% | |
| TGGCTGCTCCACGGAACTAG+TGG | - | 57115:13806-13825 | MS.gene98428:intergenic | 60.0% | |
| ! | AAGCCGACGCCGACCCATTT+TGG | - | 57115:13783-13802 | MS.gene98428:intergenic | 60.0% |
| ! | GTCGGCTTCCACTAGTTCCG+TGG | + | 57115:13795-13814 | MS.gene98428:CDS | 60.0% |
| !! | CTTTACTCGGCTCGGGTTCG+GGG | + | 57115:13597-13616 | MS.gene98428:CDS | 60.0% |
| !! | TTTACTCGGCTCGGGTTCGG+GGG | + | 57115:13598-13617 | MS.gene98428:CDS | 60.0% |
| GAGCCAGCATCAGCCCCAGA+AGG | - | 57115:13697-13716 | MS.gene98428:intergenic | 65.0% | |
| GCTCCCCCTCTAGCTGAAGG+AGG | - | 57115:13855-13874 | MS.gene98428:intergenic | 65.0% | |
| TATGGTTTCGCGGCCGGAGC+TGG | - | 57115:13826-13845 | MS.gene98428:intergenic | 65.0% | |
| TCCGCTCCCCCTCTAGCTGA+AGG | - | 57115:13858-13877 | MS.gene98428:intergenic | 65.0% | |
| TTCCGTGGAGCAGCCAGCTC+CGG | + | 57115:13810-13829 | MS.gene98428:CDS | 65.0% | |
| ! | GCCAGCGCTGAAGTTCGTCC+TGG | - | 57115:14011-14030 | MS.gene98428:intergenic | 65.0% |
| !! | GGACGAACTTCAGCGCTGGC+TGG | + | 57115:14011-14030 | MS.gene98428:CDS | 65.0% |
| ATTGGACCCTGCGGCTGCCC+CGG | - | 57115:13931-13950 | MS.gene98428:intergenic | 70.0% | |
| CAGCATCAGCCTTCCCGCCC+CGG | + | 57115:13739-13758 | MS.gene98428:CDS | 70.0% | |
| ATAGGAGCGGCGGGTCCGCC+GGG | - | 57115:13760-13779 | MS.gene98428:intergenic | 75.0% | |
| CATCAGCCTTCCCGCCCCGG+CGG | + | 57115:13742-13761 | MS.gene98428:CDS | 75.0% | |
| GAAGACCCGGGGCAGCCGCA+GGG | + | 57115:13922-13941 | MS.gene98428:CDS | 75.0% | |
| GATAGGAGCGGCGGGTCCGC+CGG | - | 57115:13761-13780 | MS.gene98428:intergenic | 75.0% | |
| GGCCGGAGCTGGCTGCTCCA+CGG | - | 57115:13815-13834 | MS.gene98428:intergenic | 75.0% | |
| TTGGACCCTGCGGCTGCCCC+GGG | - | 57115:13930-13949 | MS.gene98428:intergenic | 75.0% | |
| !! | TCGGCTCGGGTTCGGGGGTC+CGG | + | 57115:13603-13622 | MS.gene98428:CDS | 75.0% |
| ! | GGAAGACCCGGGGCAGCCGC+AGG | + | 57115:13921-13940 | MS.gene98428:CDS | 80.0% |
| ! | GTTCGGGGGTCCGGCCGCCT+TGG | + | 57115:13612-13631 | MS.gene98428:CDS | 80.0% |
| ! | TAGGAGCGGCGGGTCCGCCG+GGG | - | 57115:13759-13778 | MS.gene98428:intergenic | 80.0% |
| ! | AGCGGCGGGTCCGCCGGGGC+GGG | - | 57115:13755-13774 | MS.gene98428:intergenic | 90.0% |
| ! | GAGCGGCGGGTCCGCCGGGG+CGG | - | 57115:13756-13775 | MS.gene98428:intergenic | 90.0% |
| ! | GCGGGTCCGCCGGGGCGGGA+AGG | - | 57115:13751-13770 | MS.gene98428:intergenic | 90.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 57115 | gene | 13469 | 14101 | 13469 | ID=MS.gene98428 |
| 57115 | mRNA | 13469 | 14101 | 13469 | ID=MS.gene98428.t1;Parent=MS.gene98428 |
| 57115 | exon | 13469 | 14101 | 13469 | ID=MS.gene98428.t1.exon1;Parent=MS.gene98428.t1 |
| 57115 | CDS | 13469 | 14101 | 13469 | ID=cds.MS.gene98428.t1;Parent=MS.gene98428.t1 |
| Gene Sequence |
| Protein sequence |
