AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene98710

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene98710.t1 MTR_2g098660 93.656 331 17 2 1 329 1 329 0.0 610
MS.gene98710.t1 MTR_4g067060 56.283 382 110 8 1 327 1 380 8.32e-133 383
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene98710.t1 AT1G05900 68.696 230 68 1 87 312 141 370 1.05e-116 342
MS.gene98710.t1 AT2G31450 58.108 296 101 4 40 312 68 363 4.53e-114 335
MS.gene98710.t1 AT2G31450 58.108 296 101 4 40 312 66 361 6.15e-114 335
MS.gene98710.t1 AT1G05900 68.421 171 50 1 87 253 141 311 8.24e-81 248

Find 77 sgRNAs with CRISPR-Local

Find 203 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTGTTCAATCGAGACTTAT+TGG 0.203463 2.1:-5658855 None:intergenic
CATACCAAAAGATAATTTAT+TGG 0.207423 2.1:-5658509 None:intergenic
ACCTTGGTGTGGATTGGTTT+TGG 0.284053 2.1:-5655720 None:intergenic
CAGCGCCTTTCTAGTTTCTT+CGG 0.287332 2.1:-5658456 None:intergenic
GAAATTGAACAGGTAGGATT+TGG 0.294623 2.1:+5658731 MS.gene98710:intron
GGTTCCAATAAATTATCTTT+TGG 0.302688 2.1:+5658505 MS.gene98710:CDS
CACAACAGGAACAAATGAAT+TGG 0.309361 2.1:+5656082 MS.gene98710:CDS
ATTTGCAGCAAGTGCTTCTT+TGG 0.316813 2.1:-5655879 None:intergenic
ACTCAACGTCTTCGTCAAAA+TGG 0.325296 2.1:+5657494 MS.gene98710:CDS
ACCAAACAAACAGAAGTAAA+AGG 0.327365 2.1:-5655650 None:intergenic
GGACAATATGTACTCCTTTA+CGG 0.370840 2.1:+5658756 MS.gene98710:CDS
CATACCAAATGAGCAATCTT+AGG 0.391610 2.1:-5657885 None:intergenic
GAGTAACATGCTCCTTAGTT+TGG 0.393553 2.1:-5656643 None:intergenic
CTACCTACCTTGGTGTGGAT+TGG 0.393734 2.1:-5655726 None:intergenic
AGACTTCAAGTTCATCTTCC+AGG 0.395330 2.1:+5658831 MS.gene98710:CDS
AGCGCCTTTCTAGTTTCTTC+GGG 0.398885 2.1:-5658455 None:intergenic
GAGGTACTACTCTTACTTCC+AGG 0.400159 2.1:+5657855 MS.gene98710:CDS
AGGTCCTAAGATTGCTCATT+TGG 0.400492 2.1:+5657881 MS.gene98710:CDS
TACTACACAGAAGTTTGGTT+TGG 0.413142 2.1:+5656034 MS.gene98710:intron
GTTCTGCCCGTCAGCATTCA+AGG 0.415318 2.1:+5658808 MS.gene98710:CDS
TAGATGAAAGTCCTGCCAAT+TGG 0.428361 2.1:+5656207 MS.gene98710:intron
AAGCACTTGCTGCAAATTCA+AGG 0.437898 2.1:+5655885 MS.gene98710:CDS
CGCTGGAACGGTGGCTTCCG+AGG 0.442006 2.1:+5658474 MS.gene98710:CDS
AGTAGTACCTCAATTGAGTT+AGG 0.443051 2.1:-5657843 None:intergenic
AAAACAAACCTGCTTTGTGC+CGG 0.443349 2.1:-5658108 None:intergenic
GGTGGCTTCCGAGGGAAGAA+TGG 0.447991 2.1:+5658483 MS.gene98710:CDS
ATTTGTCTCACTAAGTATGA+TGG 0.460497 2.1:+5657813 MS.gene98710:CDS
CACCGCATCTGCAATCGTCT+TGG 0.460837 2.1:+5658071 MS.gene98710:CDS
CTTGGATGGGTTTCGAGACC+CGG 0.461852 2.1:+5658089 MS.gene98710:CDS
AAACAAACCTGCTTTGTGCC+GGG 0.463925 2.1:-5658107 None:intergenic
GCATCTGCAATCGTCTTGGA+TGG 0.472657 2.1:+5658075 MS.gene98710:CDS
CATTAGATTTAGACCTTAGG+AGG 0.472679 2.1:-5656308 None:intergenic
AGATGAAAGTCCTGCCAATT+GGG 0.477356 2.1:+5656208 MS.gene98710:intron
TTTGGTTTGGCTGAGATTGA+AGG 0.478944 2.1:+5656047 MS.gene98710:CDS
ATTGGGAAAACGTGCTTGAA+GGG 0.480580 2.1:+5656225 MS.gene98710:CDS
AATTGGGAAAACGTGCTTGA+AGG 0.485015 2.1:+5656224 MS.gene98710:CDS
TTCGAGACCCGGCACAAAGC+AGG 0.485026 2.1:+5658100 MS.gene98710:CDS
AAAACATGTTGGTCTTGTAA+TGG 0.489092 2.1:-5655693 None:intergenic
ACAACAGGAACAAATGAATT+GGG 0.495856 2.1:+5656083 MS.gene98710:CDS
GGACAGAAGATTTGCTGTCC+TGG 0.497504 2.1:+5656601 MS.gene98710:intron
GGATCTGCTGCATACACAAC+AGG 0.501474 2.1:+5656068 MS.gene98710:CDS
TCTGCACAGCGAGGCCGTAA+AGG 0.510897 2.1:-5658770 None:intergenic
CAATCGAGACTTATTGGACC+TGG 0.515302 2.1:-5658849 None:intergenic
ATTGGAACCCATTCTTCCCT+CGG 0.515483 2.1:-5658491 None:intergenic
TTTGTCTCACTAAGTATGAT+GGG 0.525399 2.1:+5657814 MS.gene98710:CDS
TCGATAAAAGAGAAGATGCC+AGG 0.527820 2.1:-5656619 None:intergenic
GAAGTCTCCTTGAATGCTGA+CGG 0.529421 2.1:-5658815 None:intergenic
CGAAGAAACTAGAAAGGCGC+TGG 0.533567 2.1:+5658457 MS.gene98710:CDS
GTGGCTTCCGAGGGAAGAAT+GGG 0.536497 2.1:+5658484 MS.gene98710:CDS
GATAGACACTACAGGTGATC+GGG 0.536871 2.1:+5656268 MS.gene98710:CDS
TGTTGATATTCATCCTCCTA+AGG 0.549271 2.1:+5656295 MS.gene98710:CDS
ATCCAAGACGATTGCAGATG+CGG 0.553059 2.1:-5658073 None:intergenic
CGATAGACACTACAGGTGAT+CGG 0.557021 2.1:+5656267 MS.gene98710:CDS
CATCTGCAATCGTCTTGGAT+GGG 0.565079 2.1:+5658076 MS.gene98710:CDS
GCTGGAACGGTGGCTTCCGA+GGG 0.566400 2.1:+5658475 MS.gene98710:CDS
AAGTCTCCTTGAATGCTGAC+GGG 0.572187 2.1:-5658814 None:intergenic
CTAGAAAGGCGCTGGAACGG+TGG 0.575424 2.1:+5658465 MS.gene98710:CDS
GCAATCTTAGGACCTACGCC+TGG 0.583177 2.1:-5657873 None:intergenic
TTGAACAGGTAGGATTTGGA+AGG 0.583390 2.1:+5658735 MS.gene98710:intron
AATACCCGAAGAAACTAGAA+AGG 0.587513 2.1:+5658451 MS.gene98710:CDS
AAACTAGAAAGGCGCTGGAA+CGG 0.595096 2.1:+5658462 MS.gene98710:CDS
GAGTATCTACACATATCCCT+TGG 0.595774 2.1:-5658043 None:intergenic
ATTGCATGGAACAATGTCCA+AGG 0.598895 2.1:+5658026 MS.gene98710:CDS
TAAGTCTCGATTGAACAAGA+AGG 0.604271 2.1:+5658859 MS.gene98710:CDS
GGACATACCTAACTCAATTG+AGG 0.608830 2.1:+5657836 MS.gene98710:CDS
ATGTATTCGATAGACACTAC+AGG 0.611197 2.1:+5656260 MS.gene98710:CDS
AGTAGCTACCTACCTTGGTG+TGG 0.611329 2.1:-5655731 None:intergenic
TCAAAATGGTCTTCTTACTG+CGG 0.633029 2.1:+5657508 MS.gene98710:CDS
CAAGACGATTGCAGATGCGG+TGG 0.636787 2.1:-5658070 None:intergenic
CGGCCTCGCTGTGCAGAGTG+TGG 0.639111 2.1:+5658776 MS.gene98710:CDS
TGTAGGTTATGATTATTGCA+TGG 0.646193 2.1:+5658012 MS.gene98710:intron
TTGCATGGAACAATGTCCAA+GGG 0.650900 2.1:+5658027 MS.gene98710:CDS
CTACTCTTACTTCCAGGCGT+AGG 0.655565 2.1:+5657861 MS.gene98710:CDS
TTGTCTCACTAAGTATGATG+GGG 0.670790 2.1:+5657815 MS.gene98710:CDS
TCCAAAACCAATCCACACCA+AGG 0.688155 2.1:+5655719 MS.gene98710:CDS
ACACCACACTCTGCACAGCG+AGG 0.705381 2.1:-5658779 None:intergenic
ACTAAGGAGCATGTTACTCG+CGG 0.708659 2.1:+5656647 MS.gene98710:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATATATGTTATAACATAA+TGG + chr2.1:5658223-5658242 MS.gene98710:intron 10.0%
!! TATATATGTTATAACATAAT+GGG + chr2.1:5658224-5658243 MS.gene98710:intron 10.0%
!! TTGAAATCAAAATAAAATAA+TGG - chr2.1:5656513-5656532 None:intergenic 10.0%
!!! AAATTTTTATGCATATAATT+AGG + chr2.1:5656580-5656599 MS.gene98710:intron 10.0%
!! AATTAAGGAAAGAAAATTTA+AGG - chr2.1:5655802-5655821 None:intergenic 15.0%
!! ATAAATTATATTGCAATACA+TGG - chr2.1:5656003-5656022 None:intergenic 15.0%
!! ATTACTATATAAGATTATCA+AGG + chr2.1:5658183-5658202 MS.gene98710:intron 15.0%
!! TATTTACATTCTCAAATTTA+TGG + chr2.1:5656676-5656695 MS.gene98710:intron 15.0%
!! TCAATTAATAAAACACAAAA+AGG - chr2.1:5657708-5657727 None:intergenic 15.0%
!! TGAAGAGATAAAAAAAATTA+AGG - chr2.1:5655817-5655836 None:intergenic 15.0%
!!! CAATTTTCATACAAAAATTT+GGG + chr2.1:5655955-5655974 MS.gene98710:intron 15.0%
!!! TATAAAGTTAAGTTATTTCT+TGG + chr2.1:5656403-5656422 MS.gene98710:intron 15.0%
!!! TCAATTTTCATACAAAAATT+TGG + chr2.1:5655954-5655973 MS.gene98710:intron 15.0%
!!! TTTAAGATTCATTTTGTTAA+TGG + chr2.1:5657950-5657969 MS.gene98710:intron 15.0%
!! AAATTGCTTAGATTAGAAAA+AGG - chr2.1:5655935-5655954 None:intergenic 20.0%
!! AATCCAAAATATAACCATTT+AGG - chr2.1:5658603-5658622 None:intergenic 20.0%
!! AATTGCTTAGATTAGAAAAA+GGG - chr2.1:5655934-5655953 None:intergenic 20.0%
!! ATGGATATGATAAAAAAACA+TGG - chr2.1:5657099-5657118 None:intergenic 20.0%
!! CATACCAAAAGATAATTTAT+TGG - chr2.1:5658512-5658531 None:intergenic 20.0%
!! CTTAAAACTAATGAAACAAA+CGG - chr2.1:5657936-5657955 None:intergenic 20.0%
!! GTACAAGAAAAAAAAAAAAC+TGG - chr2.1:5658362-5658381 None:intergenic 20.0%
!! TTACAATAATACATCATGAT+GGG + chr2.1:5656445-5656464 MS.gene98710:intron 20.0%
!!! AAATGGTTATATTTTGGATT+TGG + chr2.1:5658603-5658622 MS.gene98710:intron 20.0%
!!! AATATCTATGCTTATACTTT+TGG + chr2.1:5657727-5657746 MS.gene98710:intron 20.0%
!!! ATATCTATGCTTATACTTTT+GGG + chr2.1:5657728-5657747 MS.gene98710:intron 20.0%
!!! ATGATCAATTAATTTGGATT+TGG + chr2.1:5657620-5657639 MS.gene98710:intron 20.0%
!!! TATATTAGCTATTGACTATT+GGG + chr2.1:5656487-5656506 MS.gene98710:intron 20.0%
!!! TTATATTAGCTATTGACTAT+TGG + chr2.1:5656486-5656505 MS.gene98710:intron 20.0%
! AATAATGAGATCTCAAACTT+AGG - chr2.1:5656902-5656921 None:intergenic 25.0%
! AATACCGAAAGCTTAAAAAA+TGG - chr2.1:5657662-5657681 None:intergenic 25.0%
! ACTATATACTACATAGTCAA+AGG - chr2.1:5658551-5658570 None:intergenic 25.0%
! ACTTGAATATGAAATTGAAC+AGG + chr2.1:5658721-5658740 MS.gene98710:intron 25.0%
! ATGTTCAGAAAAAGTTTGAA+TGG - chr2.1:5657118-5657137 None:intergenic 25.0%
! ATTAGTATGTATCCATTACA+AGG + chr2.1:5657233-5657252 MS.gene98710:intron 25.0%
! ATTATCAAGGCTTATGTTAT+GGG + chr2.1:5658196-5658215 MS.gene98710:intron 25.0%
! CTGAAACTTATAGAATATTC+TGG + chr2.1:5657170-5657189 MS.gene98710:intron 25.0%
! CTTTCTATAACATGCAATAT+AGG - chr2.1:5657303-5657322 None:intergenic 25.0%
! GGAAAGAAAATTTAAGGAAA+AGG - chr2.1:5655796-5655815 None:intergenic 25.0%
! GTTACAATAATACATCATGA+TGG + chr2.1:5656444-5656463 MS.gene98710:intron 25.0%
! TCTCGTATGATCAATTAATT+TGG + chr2.1:5657614-5657633 MS.gene98710:intron 25.0%
!! AATTTTACTACACAGAAGTT+TGG + chr2.1:5656029-5656048 MS.gene98710:intron 25.0%
!! CTTTTTATGTGATCAATTCT+TGG + chr2.1:5657413-5657432 MS.gene98710:intron 25.0%
!! GGTTCCAATAAATTATCTTT+TGG + chr2.1:5658505-5658524 MS.gene98710:CDS 25.0%
!! TGAGAATTTTGTTCTTTGAT+TGG + chr2.1:5658336-5658355 MS.gene98710:intron 25.0%
!! TGCATGAAATGTATAGTTTT+GGG + chr2.1:5656126-5656145 MS.gene98710:intron 25.0%
!! TTGCATGAAATGTATAGTTT+TGG + chr2.1:5656125-5656144 MS.gene98710:intron 25.0%
!!! ATGGAAGTGTTTTTAGTTTA+GGG + chr2.1:5656162-5656181 MS.gene98710:intron 25.0%
!!! GATTCCATTTTTTAAGCTTT+CGG + chr2.1:5657655-5657674 MS.gene98710:intron 25.0%
!!! TATGGAAGTGTTTTTAGTTT+AGG + chr2.1:5656161-5656180 MS.gene98710:intron 25.0%
!!! TCAAATAAAGTCTCACTTTT+TGG - chr2.1:5655911-5655930 None:intergenic 25.0%
ACAACAGGAACAAATGAATT+GGG + chr2.1:5656083-5656102 MS.gene98710:CDS 30.0%
ACAGATGATAAACACAATTG+GGG - chr2.1:5657393-5657412 None:intergenic 30.0%
ACCAAACAAACAGAAGTAAA+AGG - chr2.1:5655653-5655672 None:intergenic 30.0%
ACCACTAACAAAATGATTTC+AGG - chr2.1:5657990-5658009 None:intergenic 30.0%
AGACAGATGATAAACACAAT+TGG - chr2.1:5657395-5657414 None:intergenic 30.0%
ATTTGTCTCACTAAGTATGA+TGG + chr2.1:5657813-5657832 MS.gene98710:CDS 30.0%
CAGTAAAGTTATCCACAATA+AGG - chr2.1:5656548-5656567 None:intergenic 30.0%
CATGACTATATGAAGTAGTT+TGG + chr2.1:5656800-5656819 MS.gene98710:intron 30.0%
CATTAAAGACATAAGCCAAA+AGG - chr2.1:5656379-5656398 None:intergenic 30.0%
CTACATTAAGAAATGCTGAA+CGG - chr2.1:5656949-5656968 None:intergenic 30.0%
CTAGATGAGAATCAACTAAA+TGG - chr2.1:5656827-5656846 None:intergenic 30.0%
GAATATGAAATTGAACAGGT+AGG + chr2.1:5658725-5658744 MS.gene98710:intron 30.0%
GACAGATGATAAACACAATT+GGG - chr2.1:5657394-5657413 None:intergenic 30.0%
GATTATCAAGGCTTATGTTA+TGG + chr2.1:5658195-5658214 MS.gene98710:intron 30.0%
TAGCATCACATTATATTTGC+TGG + chr2.1:5658139-5658158 MS.gene98710:intron 30.0%
TAGCATTAGATTTAGACCTT+AGG - chr2.1:5656314-5656333 None:intergenic 30.0%
TGTAGGTTATGATTATTGCA+TGG + chr2.1:5658012-5658031 MS.gene98710:intron 30.0%
TTAAGCTTTCGGTATTTCTT+TGG + chr2.1:5657666-5657685 MS.gene98710:intron 30.0%
TTGGACAGTTAAATTGTGTT+TGG + chr2.1:5656422-5656441 MS.gene98710:intron 30.0%
TTGTTGTATATGTAGTAGAG+GGG + chr2.1:5656870-5656889 MS.gene98710:intron 30.0%
TTTGTCTCACTAAGTATGAT+GGG + chr2.1:5657814-5657833 MS.gene98710:CDS 30.0%
! CTTGTTGTATATGTAGTAGA+GGG + chr2.1:5656869-5656888 MS.gene98710:intron 30.0%
! GAAATTAAGCACTAACCTTT+TGG + chr2.1:5656361-5656380 MS.gene98710:intron 30.0%
! GCACCTAAATGGTTATATTT+TGG + chr2.1:5658597-5658616 MS.gene98710:intron 30.0%
! TCCTGAAATCATTTTGTTAG+TGG + chr2.1:5657986-5658005 MS.gene98710:intron 30.0%
! TCCTTTTACTTCTGTTTGTT+TGG + chr2.1:5655649-5655668 MS.gene98710:CDS 30.0%
! TCTTGTTGTATATGTAGTAG+AGG + chr2.1:5656868-5656887 MS.gene98710:intron 30.0%
! TTTAGTTGATTCTCATCTAG+TGG + chr2.1:5656827-5656846 MS.gene98710:intron 30.0%
!! AAAACATGTTGGTCTTGTAA+TGG - chr2.1:5655696-5655715 None:intergenic 30.0%
!! GGGTTTTATATAAGAAAAGC+TGG + chr2.1:5657768-5657787 MS.gene98710:CDS 30.0%
!! GGTTATATTTTGGATTTGGT+GGG + chr2.1:5658607-5658626 MS.gene98710:intron 30.0%
!! TGGTTATATTTTGGATTTGG+TGG + chr2.1:5658606-5658625 MS.gene98710:intron 30.0%
!! TTTGGGTTGATTCTATTCTA+TGG + chr2.1:5656143-5656162 MS.gene98710:intron 30.0%
!!! AAGCAGGTTTGTTTTAAGTT+TGG + chr2.1:5658116-5658135 MS.gene98710:intron 30.0%
!!! GAGTGTGTTTTTTCAAGAAT+AGG + chr2.1:5657467-5657486 MS.gene98710:intron 30.0%
AAAGCTACCAAAGAACATAC+AGG - chr2.1:5657581-5657600 None:intergenic 35.0%
AACCATTTAGGTGCATTTGT+TGG - chr2.1:5658591-5658610 None:intergenic 35.0%
AAGCTACCAAAGAACATACA+GGG - chr2.1:5657580-5657599 None:intergenic 35.0%
AATACCCGAAGAAACTAGAA+AGG + chr2.1:5658451-5658470 MS.gene98710:CDS 35.0%
ACCATTTGCATTCAACTTCA+TGG - chr2.1:5657012-5657031 None:intergenic 35.0%
AGTAGTACCTCAATTGAGTT+AGG - chr2.1:5657846-5657865 None:intergenic 35.0%
ATGGTTGGACTAAGATTTAG+AGG + chr2.1:5657027-5657046 MS.gene98710:intron 35.0%
ATGTATTCGATAGACACTAC+AGG + chr2.1:5656260-5656279 MS.gene98710:CDS 35.0%
CACAACAGGAACAAATGAAT+TGG + chr2.1:5656082-5656101 MS.gene98710:CDS 35.0%
CATACCAAATGAGCAATCTT+AGG - chr2.1:5657888-5657907 None:intergenic 35.0%
CATTAGATTTAGACCTTAGG+AGG - chr2.1:5656311-5656330 None:intergenic 35.0%
CTTGTTCAATCGAGACTTAT+TGG - chr2.1:5658858-5658877 None:intergenic 35.0%
GAAATTGAACAGGTAGGATT+TGG + chr2.1:5658731-5658750 MS.gene98710:intron 35.0%
GATTTACCCTGTATGTTCTT+TGG + chr2.1:5657571-5657590 MS.gene98710:intron 35.0%
GGACAATATGTACTCCTTTA+CGG + chr2.1:5658756-5658775 MS.gene98710:CDS 35.0%
TAACAGTGTGAACCTTATTG+TGG + chr2.1:5656533-5656552 MS.gene98710:intron 35.0%
TAAGTCTCGATTGAACAAGA+AGG + chr2.1:5658859-5658878 MS.gene98710:CDS 35.0%
TACCAACAAATGCACCTAAA+TGG + chr2.1:5658586-5658605 MS.gene98710:intron 35.0%
TACTACACAGAAGTTTGGTT+TGG + chr2.1:5656034-5656053 MS.gene98710:intron 35.0%
TCAAAATGGTCTTCTTACTG+CGG + chr2.1:5657508-5657527 MS.gene98710:CDS 35.0%
TGAAGTTGAATGCAAATGGT+TGG + chr2.1:5657012-5657031 MS.gene98710:intron 35.0%
TGTTGATATTCATCCTCCTA+AGG + chr2.1:5656295-5656314 MS.gene98710:CDS 35.0%
TTGACGTGTAAGAGATGTTT+GGG + chr2.1:5657141-5657160 MS.gene98710:intron 35.0%
TTGGACTAAGATTTAGAGGA+AGG + chr2.1:5657031-5657050 MS.gene98710:intron 35.0%
TTGTCTCACTAAGTATGATG+GGG + chr2.1:5657815-5657834 MS.gene98710:CDS 35.0%
TTTGACGTGTAAGAGATGTT+TGG + chr2.1:5657140-5657159 MS.gene98710:intron 35.0%
! ACATTATATTTGCTGGCTAG+AGG + chr2.1:5658146-5658165 MS.gene98710:intron 35.0%
! CAATAATACATCATGATGGG+TGG + chr2.1:5656448-5656467 MS.gene98710:intron 35.0%
! CATTATATTTGCTGGCTAGA+GGG + chr2.1:5658147-5658166 MS.gene98710:intron 35.0%
! GGTATTGAAGTTCTCTGATA+TGG - chr2.1:5658437-5658456 None:intergenic 35.0%
! GTTTTTGCGTGTGTTGAATT+GGG - chr2.1:5655844-5655863 None:intergenic 35.0%
! TCTTTTATCGAGCCAAACTA+AGG + chr2.1:5656631-5656650 MS.gene98710:CDS 35.0%
!! ATTTATATTGACTTCGCGTG+TGG + chr2.1:5656333-5656352 MS.gene98710:intron 35.0%
!! CATTTTGTTAGTGGTGATGT+AGG + chr2.1:5657995-5658014 MS.gene98710:intron 35.0%
!! TGTTTTTGCGTGTGTTGAAT+TGG - chr2.1:5655845-5655864 None:intergenic 35.0%
!! TTGAGCATGATTTGGTGAAA+TGG + chr2.1:5657056-5657075 MS.gene98710:intron 35.0%
!!! GTTTTGGAGGAAAAACATGT+TGG - chr2.1:5655707-5655726 None:intergenic 35.0%
AAAACAAACCTGCTTTGTGC+CGG - chr2.1:5658111-5658130 None:intergenic 40.0%
AAGTTAGTAGCTACCTACCT+TGG - chr2.1:5655739-5655758 None:intergenic 40.0%
AAGTTCTCTGATATGGCATG+GGG - chr2.1:5658430-5658449 None:intergenic 40.0%
AATTGGGAAAACGTGCTTGA+AGG + chr2.1:5656224-5656243 MS.gene98710:CDS 40.0%
ACTCAACGTCTTCGTCAAAA+TGG + chr2.1:5657494-5657513 MS.gene98710:CDS 40.0%
AGATGAAAGTCCTGCCAATT+GGG + chr2.1:5656208-5656227 MS.gene98710:intron 40.0%
AGGTCCTAAGATTGCTCATT+TGG + chr2.1:5657881-5657900 MS.gene98710:CDS 40.0%
ATTGCATGGAACAATGTCCA+AGG + chr2.1:5658026-5658045 MS.gene98710:CDS 40.0%
ATTGGGAAAACGTGCTTGAA+GGG + chr2.1:5656225-5656244 MS.gene98710:CDS 40.0%
ATTTGCAGCAAGTGCTTCTT+TGG - chr2.1:5655882-5655901 None:intergenic 40.0%
CATCTAGTGGAAAGATGCTT+TGG + chr2.1:5656840-5656859 MS.gene98710:intron 40.0%
GAAGTTCTCTGATATGGCAT+GGG - chr2.1:5658431-5658450 None:intergenic 40.0%
GAGTAACATGCTCCTTAGTT+TGG - chr2.1:5656646-5656665 None:intergenic 40.0%
GAGTATCTACACATATCCCT+TGG - chr2.1:5658046-5658065 None:intergenic 40.0%
GATTGCTCATTTGGTATGTG+CGG + chr2.1:5657890-5657909 MS.gene98710:intron 40.0%
GCATTCAACTTCATGGCAAT+AGG - chr2.1:5657005-5657024 None:intergenic 40.0%
GCCATGAAGTTGAATGCAAA+TGG + chr2.1:5657008-5657027 MS.gene98710:intron 40.0%
GGACATACCTAACTCAATTG+AGG + chr2.1:5657836-5657855 MS.gene98710:CDS 40.0%
TAGATGAAAGTCCTGCCAAT+TGG + chr2.1:5656207-5656226 MS.gene98710:intron 40.0%
TCGATAAAAGAGAAGATGCC+AGG - chr2.1:5656622-5656641 None:intergenic 40.0%
TGAAGTTCTCTGATATGGCA+TGG - chr2.1:5658432-5658451 None:intergenic 40.0%
TTGAACAGGTAGGATTTGGA+AGG + chr2.1:5658735-5658754 MS.gene98710:intron 40.0%
TTGCATGGAACAATGTCCAA+GGG + chr2.1:5658027-5658046 MS.gene98710:CDS 40.0%
! AAGCACTTGCTGCAAATTCA+AGG + chr2.1:5655885-5655904 MS.gene98710:CDS 40.0%
! AAGTCTTGATTTTGCTCCAG+AGG + chr2.1:5656971-5656990 MS.gene98710:intron 40.0%
! AGACTTCAAGTTCATCTTCC+AGG + chr2.1:5658831-5658850 MS.gene98710:CDS 40.0%
! CTTTTGGGCATTCTTTTCTC+AGG + chr2.1:5657743-5657762 MS.gene98710:intron 40.0%
! TTCAAGCACGTTTTCCCAAT+TGG - chr2.1:5656225-5656244 None:intergenic 40.0%
!! CATGATGGGTGGATTGTTAT+TGG + chr2.1:5656459-5656478 MS.gene98710:intron 40.0%
!! TTTGGTTTGGCTGAGATTGA+AGG + chr2.1:5656047-5656066 MS.gene98710:CDS 40.0%
!!! GGAAGGTTTTGAGCATGATT+TGG + chr2.1:5657048-5657067 MS.gene98710:intron 40.0%
AAACAAACCTGCTTTGTGCC+GGG - chr2.1:5658110-5658129 None:intergenic 45.0%
AAACCAATCCACACCAAGGT+AGG + chr2.1:5655723-5655742 MS.gene98710:intron 45.0%
AAGTCTCCTTGAATGCTGAC+GGG - chr2.1:5658817-5658836 None:intergenic 45.0%
AGCAGCCATTATGTGCACTT+TGG + chr2.1:5657366-5657385 MS.gene98710:intron 45.0%
ATCCAAGACGATTGCAGATG+CGG - chr2.1:5658076-5658095 None:intergenic 45.0%
ATTGGAACCCATTCTTCCCT+CGG - chr2.1:5658494-5658513 None:intergenic 45.0%
CAATCGAGACTTATTGGACC+TGG - chr2.1:5658852-5658871 None:intergenic 45.0%
CATCTGCAATCGTCTTGGAT+GGG + chr2.1:5658076-5658095 MS.gene98710:CDS 45.0%
CGATAGACACTACAGGTGAT+CGG + chr2.1:5656267-5656286 MS.gene98710:CDS 45.0%
GAAGTCTCCTTGAATGCTGA+CGG - chr2.1:5658818-5658837 None:intergenic 45.0%
GATAGACACTACAGGTGATC+GGG + chr2.1:5656268-5656287 MS.gene98710:CDS 45.0%
GCAATAGGTTTCTCATCCTC+TGG - chr2.1:5656990-5657009 None:intergenic 45.0%
TCCAAAACCAATCCACACCA+AGG + chr2.1:5655719-5655738 MS.gene98710:CDS 45.0%
TTGCGCTTTCTTTCTGTGTC+AGG - chr2.1:5657259-5657278 None:intergenic 45.0%
! ACTAAGGAGCATGTTACTCG+CGG + chr2.1:5656647-5656666 MS.gene98710:CDS 45.0%
! GAGGTACTACTCTTACTTCC+AGG + chr2.1:5657855-5657874 MS.gene98710:CDS 45.0%
! GGGCATTCTTTTCTCAGGTT+GGG + chr2.1:5657748-5657767 MS.gene98710:intron 45.0%
! TGGGCATTCTTTTCTCAGGT+TGG + chr2.1:5657747-5657766 MS.gene98710:intron 45.0%
! TTCTGTGTCAGGCCTTGTAA+TGG - chr2.1:5657248-5657267 None:intergenic 45.0%
!! AAACTAGAAAGGCGCTGGAA+CGG + chr2.1:5658462-5658481 MS.gene98710:CDS 45.0%
!! AGCGCCTTTCTAGTTTCTTC+GGG - chr2.1:5658458-5658477 None:intergenic 45.0%
!! CAGCGCCTTTCTAGTTTCTT+CGG - chr2.1:5658459-5658478 None:intergenic 45.0%
!! TTCTCAGTGCTGACAAACAC+CGG - chr2.1:5658322-5658341 None:intergenic 45.0%
!!! ACCTTGGTGTGGATTGGTTT+TGG - chr2.1:5655723-5655742 None:intergenic 45.0%
!!! TTGGTGTGGATTGGTTTTGG+AGG - chr2.1:5655720-5655739 None:intergenic 45.0%
AGTAGCTACCTACCTTGGTG+TGG - chr2.1:5655734-5655753 None:intergenic 50.0%
CTACTCTTACTTCCAGGCGT+AGG + chr2.1:5657861-5657880 MS.gene98710:CDS 50.0%
GCATCTGCAATCGTCTTGGA+TGG + chr2.1:5658075-5658094 MS.gene98710:CDS 50.0%
GGATCTGCTGCATACACAAC+AGG + chr2.1:5656068-5656087 MS.gene98710:CDS 50.0%
GGGGTCCAAAGTGCACATAA+TGG - chr2.1:5657374-5657393 None:intergenic 50.0%
TCTGATATGGCATGGGGAAG+AGG - chr2.1:5658424-5658443 None:intergenic 50.0%
! AGCACGTTTTCCCAATTGGC+AGG - chr2.1:5656221-5656240 None:intergenic 50.0%
! CGAAGAAACTAGAAAGGCGC+TGG + chr2.1:5658457-5658476 MS.gene98710:CDS 50.0%
! CTACCTACCTTGGTGTGGAT+TGG - chr2.1:5655729-5655748 None:intergenic 50.0%
!! GGACAGAAGATTTGCTGTCC+TGG + chr2.1:5656601-5656620 MS.gene98710:intron 50.0%
CAAGACGATTGCAGATGCGG+TGG - chr2.1:5658073-5658092 None:intergenic 55.0%
CACCGCATCTGCAATCGTCT+TGG + chr2.1:5658071-5658090 MS.gene98710:CDS 55.0%
GCAATCTTAGGACCTACGCC+TGG - chr2.1:5657876-5657895 None:intergenic 55.0%
GTGGCTTCCGAGGGAAGAAT+GGG + chr2.1:5658484-5658503 MS.gene98710:CDS 55.0%
GTTCTGCCCGTCAGCATTCA+AGG + chr2.1:5658808-5658827 MS.gene98710:CDS 55.0%
! CTTGGATGGGTTTCGAGACC+CGG + chr2.1:5658089-5658108 MS.gene98710:CDS 55.0%
!! TGCTTGACTTGCGAGTCAGC+CGG + chr2.1:5658300-5658319 MS.gene98710:intron 55.0%
ACACCACACTCTGCACAGCG+AGG - chr2.1:5658782-5658801 None:intergenic 60.0%
GGTGGCTTCCGAGGGAAGAA+TGG + chr2.1:5658483-5658502 MS.gene98710:CDS 60.0%
TCTGCACAGCGAGGCCGTAA+AGG - chr2.1:5658773-5658792 None:intergenic 60.0%
TTCGAGACCCGGCACAAAGC+AGG + chr2.1:5658100-5658119 MS.gene98710:CDS 60.0%
!! CTAGAAAGGCGCTGGAACGG+TGG + chr2.1:5658465-5658484 MS.gene98710:CDS 60.0%
GCTGGAACGGTGGCTTCCGA+GGG + chr2.1:5658475-5658494 MS.gene98710:CDS 65.0%
CGCTGGAACGGTGGCTTCCG+AGG + chr2.1:5658474-5658493 MS.gene98710:CDS 70.0%
CGGCCTCGCTGTGCAGAGTG+TGG + chr2.1:5658776-5658795 MS.gene98710:CDS 70.0%


Chromosome Type Strat End Strand Name
chr2.1 gene 5655638 5658889 5655638 ID=MS.gene98710
chr2.1 mRNA 5655638 5658889 5655638 ID=MS.gene98710.t1;Parent=MS.gene98710
chr2.1 exon 5655638 5655740 5655638 ID=MS.gene98710.t1.exon1;Parent=MS.gene98710.t1
chr2.1 CDS 5655638 5655740 5655638 ID=cds.MS.gene98710.t1;Parent=MS.gene98710.t1
chr2.1 exon 5655838 5655914 5655838 ID=MS.gene98710.t1.exon2;Parent=MS.gene98710.t1
chr2.1 CDS 5655838 5655914 5655838 ID=cds.MS.gene98710.t1;Parent=MS.gene98710.t1
chr2.1 exon 5656044 5656113 5656044 ID=MS.gene98710.t1.exon3;Parent=MS.gene98710.t1
chr2.1 CDS 5656044 5656113 5656044 ID=cds.MS.gene98710.t1;Parent=MS.gene98710.t1
chr2.1 exon 5656210 5656316 5656210 ID=MS.gene98710.t1.exon4;Parent=MS.gene98710.t1
chr2.1 CDS 5656210 5656316 5656210 ID=cds.MS.gene98710.t1;Parent=MS.gene98710.t1
chr2.1 exon 5656602 5656668 5656602 ID=MS.gene98710.t1.exon5;Parent=MS.gene98710.t1
chr2.1 CDS 5656602 5656668 5656602 ID=cds.MS.gene98710.t1;Parent=MS.gene98710.t1
chr2.1 exon 5657489 5657580 5657489 ID=MS.gene98710.t1.exon6;Parent=MS.gene98710.t1
chr2.1 CDS 5657489 5657580 5657489 ID=cds.MS.gene98710.t1;Parent=MS.gene98710.t1
chr2.1 exon 5657765 5657902 5657765 ID=MS.gene98710.t1.exon7;Parent=MS.gene98710.t1
chr2.1 CDS 5657765 5657902 5657765 ID=cds.MS.gene98710.t1;Parent=MS.gene98710.t1
chr2.1 exon 5658017 5658121 5658017 ID=MS.gene98710.t1.exon8;Parent=MS.gene98710.t1
chr2.1 CDS 5658017 5658121 5658017 ID=cds.MS.gene98710.t1;Parent=MS.gene98710.t1
chr2.1 exon 5658443 5658526 5658443 ID=MS.gene98710.t1.exon9;Parent=MS.gene98710.t1
chr2.1 CDS 5658443 5658526 5658443 ID=cds.MS.gene98710.t1;Parent=MS.gene98710.t1
chr2.1 exon 5658743 5658889 5658743 ID=MS.gene98710.t1.exon10;Parent=MS.gene98710.t1
chr2.1 CDS 5658743 5658889 5658743 ID=cds.MS.gene98710.t1;Parent=MS.gene98710.t1
Gene Sequence

>MS.gene98710

ATGTTTGTGTTTCCTTTTACTTCTGTTTGTTTGGTGCGAATCTCTGTCTTCTCTTCCATTACAAGACCAACATGTTTTTCCTCCAAAACCAATCCACACCAAGGTAGGTAGCTACTAACTTACTTCTTCACAATACATTCTTATTATTTTCAATTCCTTTTCCTTAAATTTTCTTTCCTTAATTTTTTTTATCTCTTCAGTGTCCCAATTCAACACACGCAAAAACACTACACAACTTCAACCAAAGAAGCACTTGCTGCAAATTCAAGGCCAAAAAGTGAGACTTTATTTGACCCTTTTTCTAATCTAAGCAATTTCAATTTTCATACAAAAATTTGGGAATTCTTCAAATGTTCACATTTCCATGTATTGCAATATAATTTATGTTAGTAATTTTACTACACAGAAGTTTGGTTTGGCTGAGATTGAAGGATCTGCTGCATACACAACAGGAACAAATGAATTGGGTCAAACTAGTAAGTGCTTCTTGCATGAAATGTATAGTTTTGGGTTGATTCTATTCTATGGAAGTGTTTTTAGTTTAGGGAGCTTATAGTGCTTTATTTATGTAGATGAAAGTCCTGCCAATTGGGAAAACGTGCTTGAAGGGATTCGCAAAATGATGTATTCGATAGACACTACAGGTGATCGGGAAGATGTTGATATTCATCCTCCTAAGGTCTAAATCTAATGCTATTTATATTGACTTCGCGTGTGGTGAAAGAAATTAAGCACTAACCTTTTGGCTTATGTCTTTAATGTGATTATAAAGTTAAGTTATTTCTTGGACAGTTAAATTGTGTTTGGTTACAATAATACATCATGATGGGTGGATTGTTATTGGAAATTTATATTAGCTATTGACTATTGGGCCATTATTTTATTTTGATTTCAATAACAGTGTGAACCTTATTGTGGATAACTTTACTGTCATAATAAACTAAATTTTTATGCATATAATTAGGACAGAAGATTTGCTGTCCTGGCATCTTCTCTTTTATCGAGCCAAACTAAGGAGCATGTTACTCGCGGTAAGCATATTTACATTCTCAAATTTATGGTCATGAAAAACATGTCGAGATTTGTTTTCTTTTGTTCGTAGTTTGAGAAGCTTCATTCTAAGATTGTAATGTTATTTTTCGATAATATTTATGATTCATGACATGACTATATGAAGTAGTTTGGTCCATTTAGTTGATTCTCATCTAGTGGAAAGATGCTTTGGTTCGTTCTTGTTGTATATGTAGTAGAGGGGAGCATACCTAAGTTTGAGATCTCATTATTTGACGTTTACAATTTGATAAAGCACCGTTCAGCATTTCTTAATGTAGCTAAGTCTTGATTTTGCTCCAGAGGATGAGAAACCTATTGCCATGAAGTTGAATGCAAATGGTTGGACTAAGATTTAGAGGAAGGTTTTGAGCATGATTTGGTGAAATGGAAACAGATTTAGCATTTCCATGTTTTTTTATCATATCCATTCAAACTTTTTCTGAACATTTTTTGACGTGTAAGAGATGTTTGGGAAGCTTCTGAAACTTATAGAATATTCTGGAATACACACAGAAAAATACAACATCACATCAGAAATAAAAATTAGTATGTATCCATTACAAGGCCTGACACAGAAAGAAAGCGCAAAAAAAAAAAAATTAGATACTTCCTATATTGCATGTTATAGAAAGCTGAACTTCAAAAATCAATGAAGTGCGAATCTAACTAAATTTGAGCAGCCATTATGTGCACTTTGGACCCCAATTGTGTTTATCATCTGTCTTTTTATGTGATCAATTCTTGGTAGTTTCTCGTACAAATTTAGTCATATTTAAGAGTGTGTTTTTTCAAGAATAGGAGCAACTCAACGTCTTCGTCAAAATGGTCTTCTTACTGCGGATGCACTCAACAAAGCTGATGAAGAAACTATCAAAAAACTGATTTACCCTGTATGTTCTTTGGTAGCTTTTGAAAACTGTGCTTCTCGTATGATCAATTAATTTGGATTTGGTAGAAAATTAAAGATTCCATTTTTTAAGCTTTCGGTATTTCTTTGGTCAAATTATTTTATGTCCTTTTTGTGTTTTATTAATTGAATATCTATGCTTATACTTTTGGGCATTCTTTTCTCAGGTTGGGTTTTATATAAGAAAAGCTGGTAACTTGAAGAAAATTGCTGATATTTGTCTCACTAAGTATGATGGGGACATACCTAACTCAATTGAGGTACTACTCTTACTTCCAGGCGTAGGTCCTAAGATTGCTCATTTGGTATGTGCGGTTTTCTTCTCGTATTTCAAACCGTTTGTTTCATTAGTTTTAAGATTCATTTTGTTAATGGTATTAGTTTTATTTCCTGAAATCATTTTGTTAGTGGTGATGTAGGTTATGATTATTGCATGGAACAATGTCCAAGGGATATGTGTAGATACTCATGTCCACCGCATCTGCAATCGTCTTGGATGGGTTTCGAGACCCGGCACAAAGCAGGTTTGTTTTAAGTTTGGTAGCATCACATTATATTTGCTGGCTAGAGGGAAAAATTATGCAAATTACTATATAAGATTATCAAGGCTTATGTTATGGGATCAATATATATGTTATAACATAATGGGAATGAAATTTTATTATCAGAACGTATCTTTTATTTATCATGATGTCTGAAATTTGCTTGACTTGCGAGTCAGCCGGTGTTTGTCAGCACTGAGAATTTTGTTCTTTGATTGGCCAGTTTTTTTTTTTTCTTGTACTAACTTGTTTCTAAGTGTCACAAATTCAAAGTTAATAATCCTCTTCCCCATGCCATATCAGAGAACTTCAATACCCGAAGAAACTAGAAAGGCGCTGGAACGGTGGCTTCCGAGGGAAGAATGGGTTCCAATAAATTATCTTTTGGTATGCATAGCTCATTCACTTCCTTTGACTATGTAGTATATAGTATAGACTATAGTATATACCAACAAATGCACCTAAATGGTTATATTTTGGATTTGGTGGGTTGTATTATTACTGCATTGCTAAAAACATTTTTGAGCTTCTTATATCATCTATCTTACAACATTGTTTGCTTATATGTAAAGCATCATCTGACTTGAATATGAAATTGAACAGGTAGGATTTGGAAGGACAATATGTACTCCTTTACGGCCTCGCTGTGCAGAGTGTGGTGTAAGTAAGTTCTGCCCGTCAGCATTCAAGGAGACTTCAAGTTCATCTTCCAGGTCCAATAAGTCTCGATTGAACAAGAAGGCTGAGTGA
Protein sequence

>MS.gene98710.t1

MFVFPFTSVCLVRISVFSSITRPTCFSSKTNPHQVSQFNTRKNTTQLQPKKHLLQIQGQKKFGLAEIEGSAAYTTGTNELGQTNESPANWENVLEGIRKMMYSIDTTGDREDVDIHPPKDRRFAVLASSLLSSQTKEHVTRGATQRLRQNGLLTADALNKADEETIKKLIYPVGFYIRKAGNLKKIADICLTKYDGDIPNSIEVLLLLPGVGPKIAHLVMIIAWNNVQGICVDTHVHRICNRLGWVSRPGTKQRTSIPEETRKALERWLPREEWVPINYLLVGFGRTICTPLRPRCAECGVSKFCPSAFKETSSSSSRSNKSRLNKKAE