Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98843.t1 | AES76709.1 | 100 | 99 | 0 | 0 | 1 | 99 | 1 | 99 | 4.30E-54 | 220.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98843.t1 | Q94C12 | 78.4 | 97 | 21 | 0 | 1 | 97 | 1 | 97 | 2.3e-43 | 176.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98843.t1 | G7KNF5 | 100.0 | 99 | 0 | 0 | 1 | 99 | 1 | 99 | 3.1e-54 | 220.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05462 | MS.gene98843 | 0.818101 | 2.34E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98843.t1 | MTR_6g084520 | 98.039 | 102 | 2 | 0 | 1 | 102 | 1 | 102 | 1.65e-72 | 211 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98843.t1 | AT3G18410 | 78.351 | 97 | 21 | 0 | 1 | 97 | 1 | 97 | 2.04e-55 | 167 |
| MS.gene98843.t1 | AT3G18410 | 78.351 | 97 | 21 | 0 | 1 | 97 | 1 | 97 | 2.04e-55 | 167 |
| MS.gene98843.t1 | AT1G49140 | 76.289 | 97 | 23 | 0 | 1 | 97 | 1 | 97 | 4.50e-54 | 164 |
| MS.gene98843.t1 | AT1G49140 | 76.289 | 97 | 23 | 0 | 1 | 97 | 1 | 97 | 4.50e-54 | 164 |
Find 27 sgRNAs with CRISPR-Local
Find 29 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAAGCTTCGTTGGTGTTAT+AGG | 0.264593 | 6.1:+74008932 | MS.gene98843:CDS |
| GGTCGTCACCCTTCTCTTCA+TGG | 0.286257 | 6.1:+74009042 | MS.gene98843:CDS |
| AGTCTACTAGAGGGATTGGT+TGG | 0.361455 | 6.1:+74009010 | MS.gene98843:CDS |
| ATCGCCACTGGATCTTTGTA+TGG | 0.363135 | 6.1:-74008841 | None:intergenic |
| CAAAGATCCAGTGGCGATGC+TGG | 0.412298 | 6.1:+74008846 | MS.gene98843:CDS |
| CGTTGGTGTTATAGGATTGA+AGG | 0.432641 | 6.1:+74008940 | MS.gene98843:CDS |
| GTCTACTAGAGGGATTGGTT+GGG | 0.443343 | 6.1:+74009011 | MS.gene98843:CDS |
| CTCATTTCCAGCATCGCCAC+TGG | 0.446593 | 6.1:-74008853 | None:intergenic |
| CTTGAGTCTACTAGAGGGAT+TGG | 0.477507 | 6.1:+74009006 | MS.gene98843:CDS |
| GGGAAGGAAGAAGGGGTATG+TGG | 0.484400 | 6.1:+74008777 | MS.gene98843:CDS |
| TCTACTAGAGGGATTGGTTG+GGG | 0.490687 | 6.1:+74009012 | MS.gene98843:CDS |
| CAGTGGCGATGCTGGAAATG+AGG | 0.508085 | 6.1:+74008854 | MS.gene98843:CDS |
| TTCGCCGGATCGAAATCATC+TGG | 0.510493 | 6.1:-74008817 | None:intergenic |
| GGATCTTTGTATGGGTTCGC+CGG | 0.524941 | 6.1:-74008832 | None:intergenic |
| TCGCCACTGGATCTTTGTAT+GGG | 0.545031 | 6.1:-74008840 | None:intergenic |
| GTTGGTGTTATAGGATTGAA+GGG | 0.554470 | 6.1:+74008941 | MS.gene98843:CDS |
| AAACACAAACCATGAAGAGA+AGG | 0.554780 | 6.1:-74009051 | None:intergenic |
| TTATTCGTGAAAAGCTTCGT+TGG | 0.561335 | 6.1:+74008923 | MS.gene98843:CDS |
| GCCGGATCGAAATCATCTGG+TGG | 0.568519 | 6.1:-74008814 | None:intergenic |
| AGTGGCGATGCTGGAAATGA+GGG | 0.574162 | 6.1:+74008855 | MS.gene98843:CDS |
| CCCACCAGATGATTTCGATC+CGG | 0.583849 | 6.1:+74008813 | MS.gene98843:CDS |
| CAGTATCTTGAGTCTACTAG+AGG | 0.595830 | 6.1:+74009000 | MS.gene98843:CDS |
| CTACTAGAGGGATTGGTTGG+GGG | 0.604522 | 6.1:+74009013 | MS.gene98843:CDS |
| CCGGATCGAAATCATCTGGT+GGG | 0.615720 | 6.1:-74008813 | None:intergenic |
| AACACAAACCATGAAGAGAA+GGG | 0.648401 | 6.1:-74009050 | None:intergenic |
| AGTATCTTGAGTCTACTAGA+GGG | 0.663002 | 6.1:+74009001 | MS.gene98843:CDS |
| GAACCCATACAAAGATCCAG+TGG | 0.708004 | 6.1:+74008837 | MS.gene98843:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AACACATTGTTCGTGAAAAA+TGG | + | chr6.1:74008878-74008897 | MS.gene98843:CDS | 30.0% | |
| AAACACAAACCATGAAGAGA+AGG | - | chr6.1:74009054-74009073 | None:intergenic | 35.0% | |
| AACACAAACCATGAAGAGAA+GGG | - | chr6.1:74009053-74009072 | None:intergenic | 35.0% | |
| AGTATCTTGAGTCTACTAGA+GGG | + | chr6.1:74009001-74009020 | MS.gene98843:CDS | 35.0% | |
| TTATTCGTGAAAAGCTTCGT+TGG | + | chr6.1:74008923-74008942 | MS.gene98843:CDS | 35.0% | |
| !! | AAAAGCTTCGTTGGTGTTAT+AGG | + | chr6.1:74008932-74008951 | MS.gene98843:CDS | 35.0% |
| !! | GTTGGTGTTATAGGATTGAA+GGG | + | chr6.1:74008941-74008960 | MS.gene98843:CDS | 35.0% |
| CAGTATCTTGAGTCTACTAG+AGG | + | chr6.1:74009000-74009019 | MS.gene98843:CDS | 40.0% | |
| !! | CGTTGGTGTTATAGGATTGA+AGG | + | chr6.1:74008940-74008959 | MS.gene98843:CDS | 40.0% |
| ATCGCCACTGGATCTTTGTA+TGG | - | chr6.1:74008844-74008863 | None:intergenic | 45.0% | |
| CTTGAGTCTACTAGAGGGAT+TGG | + | chr6.1:74009006-74009025 | MS.gene98843:CDS | 45.0% | |
| GAACCCATACAAAGATCCAG+TGG | + | chr6.1:74008837-74008856 | MS.gene98843:CDS | 45.0% | |
| TCGCCACTGGATCTTTGTAT+GGG | - | chr6.1:74008843-74008862 | None:intergenic | 45.0% | |
| ! | AGTCTACTAGAGGGATTGGT+TGG | + | chr6.1:74009010-74009029 | MS.gene98843:CDS | 45.0% |
| !! | GTCTACTAGAGGGATTGGTT+GGG | + | chr6.1:74009011-74009030 | MS.gene98843:CDS | 45.0% |
| !! | TCTACTAGAGGGATTGGTTG+GGG | + | chr6.1:74009012-74009031 | MS.gene98843:CDS | 45.0% |
| AGTGGCGATGCTGGAAATGA+GGG | + | chr6.1:74008855-74008874 | MS.gene98843:CDS | 50.0% | |
| CCCACCAGATGATTTCGATC+CGG | + | chr6.1:74008813-74008832 | MS.gene98843:CDS | 50.0% | |
| CCGGATCGAAATCATCTGGT+GGG | - | chr6.1:74008816-74008835 | None:intergenic | 50.0% | |
| GGATCTTTGTATGGGTTCGC+CGG | - | chr6.1:74008835-74008854 | None:intergenic | 50.0% | |
| TTCGCCGGATCGAAATCATC+TGG | - | chr6.1:74008820-74008839 | None:intergenic | 50.0% | |
| !! | CTACTAGAGGGATTGGTTGG+GGG | + | chr6.1:74009013-74009032 | MS.gene98843:CDS | 50.0% |
| CAAAGATCCAGTGGCGATGC+TGG | + | chr6.1:74008846-74008865 | MS.gene98843:CDS | 55.0% | |
| CAGTGGCGATGCTGGAAATG+AGG | + | chr6.1:74008854-74008873 | MS.gene98843:CDS | 55.0% | |
| CTCATTTCCAGCATCGCCAC+TGG | - | chr6.1:74008856-74008875 | None:intergenic | 55.0% | |
| GCCGGATCGAAATCATCTGG+TGG | - | chr6.1:74008817-74008836 | None:intergenic | 55.0% | |
| GGTCGTCACCCTTCTCTTCA+TGG | + | chr6.1:74009042-74009061 | MS.gene98843:CDS | 55.0% | |
| ! | GGGAAGGAAGAAGGGGTATG+TGG | + | chr6.1:74008777-74008796 | MS.gene98843:CDS | 55.0% |
| !! | GGGATTGGTTGGGGGAAAGA+TGG | + | chr6.1:74009021-74009040 | MS.gene98843:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.1 | gene | 74008775 | 74009104 | 74008775 | ID=MS.gene98843 |
| chr6.1 | mRNA | 74008775 | 74009104 | 74008775 | ID=MS.gene98843.t1;Parent=MS.gene98843 |
| chr6.1 | exon | 74008775 | 74009104 | 74008775 | ID=MS.gene98843.t1.exon1;Parent=MS.gene98843.t1 |
| chr6.1 | CDS | 74008775 | 74009104 | 74008775 | ID=cds.MS.gene98843.t1;Parent=MS.gene98843.t1 |
| Gene Sequence |
| Protein sequence |
