AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene05462


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene05462.t1 MTR_6g084520 94.231 104 6 0 1 104 1 104 7.74e-70 204
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene05462.t1 AT3G18410 78.125 96 21 0 1 96 1 96 2.04e-54 165
MS.gene05462.t1 AT3G18410 78.125 96 21 0 1 96 1 96 2.04e-54 165
MS.gene05462.t1 AT1G49140 76.042 96 23 0 1 96 1 96 4.49e-53 162
MS.gene05462.t1 AT1G49140 76.042 96 23 0 1 96 1 96 4.49e-53 162

Find 28 sgRNAs with CRISPR-Local

Find 36 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAAAGCTTCGTTGGTGTTAT+AGG 0.264593 6.4:+58334284 MS.gene05462:CDS
GGTCGTCACCCTTCTCTTCA+TGG 0.343983 6.4:+58334394 MS.gene05462:CDS
AGTCTACTAGAGGGATTGGT+TGG 0.361455 6.4:+58334362 MS.gene05462:CDS
ATCGCCACTGGATCTTTGTA+TGG 0.363135 6.4:-58334193 None:intergenic
TTGTTGATTAAGTGCATGTT+TGG 0.392595 6.4:+58334545 MS.gene05462:CDS
CAAAGATCCAGTGGCGATGC+TGG 0.412298 6.4:+58334198 MS.gene05462:CDS
CGTTGGTGTTATAGGATTGA+AGG 0.432641 6.4:+58334292 MS.gene05462:CDS
GTCTACTAGAGGGATTGGTT+GGG 0.443343 6.4:+58334363 MS.gene05462:CDS
CTCATTTCCAGCATCGCCAC+TGG 0.446593 6.4:-58334205 None:intergenic
CTTGAGTCTACTAGAGGGAT+TGG 0.477507 6.4:+58334358 MS.gene05462:CDS
GGGAAGGAAGAAGGGGTATG+TGG 0.484400 6.4:+58334129 MS.gene05462:CDS
TCTACTAGAGGGATTGGTTG+GGG 0.490687 6.4:+58334364 MS.gene05462:CDS
CAGTGGCGATGCTGGAAATG+AGG 0.508085 6.4:+58334206 MS.gene05462:CDS
TTCGCCGGATCGAAATCATC+TGG 0.510493 6.4:-58334169 None:intergenic
GGATCTTTGTATGGGTTCGC+CGG 0.524941 6.4:-58334184 None:intergenic
TCGCCACTGGATCTTTGTAT+GGG 0.545031 6.4:-58334192 None:intergenic
GTTGGTGTTATAGGATTGAA+GGG 0.554470 6.4:+58334293 MS.gene05462:CDS
AAACACAGACCATGAAGAGA+AGG 0.559518 6.4:-58334403 None:intergenic
TTATTCGTGAAAAGCTTCGT+TGG 0.561335 6.4:+58334275 MS.gene05462:CDS
GCCGGATCGAAATCATCTGG+TGG 0.568519 6.4:-58334166 None:intergenic
AGTGGCGATGCTGGAAATGA+GGG 0.574162 6.4:+58334207 MS.gene05462:CDS
CCCACCAGATGATTTCGATC+CGG 0.583849 6.4:+58334165 MS.gene05462:CDS
CAGTATCTTGAGTCTACTAG+AGG 0.595830 6.4:+58334352 MS.gene05462:CDS
CTACTAGAGGGATTGGTTGG+GGG 0.604522 6.4:+58334365 MS.gene05462:CDS
CCGGATCGAAATCATCTGGT+GGG 0.615720 6.4:-58334165 None:intergenic
AACACAGACCATGAAGAGAA+GGG 0.638042 6.4:-58334402 None:intergenic
AGTATCTTGAGTCTACTAGA+GGG 0.663002 6.4:+58334353 MS.gene05462:CDS
GAACCCATACAAAGATCCAG+TGG 0.708004 6.4:+58334189 MS.gene05462:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTGTTGTGAAATTATGAATT+GGG + chr6.4:58334464-58334483 MS.gene05462:intron 20.0%
!!! GATTTTTATGTAGTATTGAA+GGG + chr6.4:58334512-58334531 MS.gene05462:intron 20.0%
!!! TGATTTTTATGTAGTATTGA+AGG + chr6.4:58334511-58334530 MS.gene05462:intron 20.0%
!!! TTTGTTGTGAAATTATGAAT+TGG + chr6.4:58334463-58334482 MS.gene05462:intron 20.0%
!! ATTCTCTTTTGTTCTCTTTT+TGG + chr6.4:58334427-58334446 MS.gene05462:intron 25.0%
!!! TTCTCTTTTTGGTGTATAAT+TGG + chr6.4:58334438-58334457 MS.gene05462:intron 25.0%
AACACATTGTTCGTGAAAAA+TGG + chr6.4:58334230-58334249 MS.gene05462:CDS 30.0%
! TTGTTGATTAAGTGCATGTT+TGG + chr6.4:58334545-58334564 MS.gene05462:CDS 30.0%
AGTATCTTGAGTCTACTAGA+GGG + chr6.4:58334353-58334372 MS.gene05462:CDS 35.0%
TTATTCGTGAAAAGCTTCGT+TGG + chr6.4:58334275-58334294 MS.gene05462:CDS 35.0%
!! AAAAGCTTCGTTGGTGTTAT+AGG + chr6.4:58334284-58334303 MS.gene05462:CDS 35.0%
!! GTTGGTGTTATAGGATTGAA+GGG + chr6.4:58334293-58334312 MS.gene05462:CDS 35.0%
AAACACAGACCATGAAGAGA+AGG - chr6.4:58334406-58334425 None:intergenic 40.0%
AACACAGACCATGAAGAGAA+GGG - chr6.4:58334405-58334424 None:intergenic 40.0%
CAGTATCTTGAGTCTACTAG+AGG + chr6.4:58334352-58334371 MS.gene05462:CDS 40.0%
!! CGTTGGTGTTATAGGATTGA+AGG + chr6.4:58334292-58334311 MS.gene05462:CDS 40.0%
ATCGCCACTGGATCTTTGTA+TGG - chr6.4:58334196-58334215 None:intergenic 45.0%
CTTGAGTCTACTAGAGGGAT+TGG + chr6.4:58334358-58334377 MS.gene05462:CDS 45.0%
GAACCCATACAAAGATCCAG+TGG + chr6.4:58334189-58334208 MS.gene05462:CDS 45.0%
TCGCCACTGGATCTTTGTAT+GGG - chr6.4:58334195-58334214 None:intergenic 45.0%
! AGTCTACTAGAGGGATTGGT+TGG + chr6.4:58334362-58334381 MS.gene05462:CDS 45.0%
!! GTCTACTAGAGGGATTGGTT+GGG + chr6.4:58334363-58334382 MS.gene05462:CDS 45.0%
!! TCTACTAGAGGGATTGGTTG+GGG + chr6.4:58334364-58334383 MS.gene05462:CDS 45.0%
AGTGGCGATGCTGGAAATGA+GGG + chr6.4:58334207-58334226 MS.gene05462:CDS 50.0%
CCCACCAGATGATTTCGATC+CGG + chr6.4:58334165-58334184 MS.gene05462:CDS 50.0%
CCGGATCGAAATCATCTGGT+GGG - chr6.4:58334168-58334187 None:intergenic 50.0%
GGATCTTTGTATGGGTTCGC+CGG - chr6.4:58334187-58334206 None:intergenic 50.0%
TTCGCCGGATCGAAATCATC+TGG - chr6.4:58334172-58334191 None:intergenic 50.0%
!! CTACTAGAGGGATTGGTTGG+GGG + chr6.4:58334365-58334384 MS.gene05462:CDS 50.0%
CAAAGATCCAGTGGCGATGC+TGG + chr6.4:58334198-58334217 MS.gene05462:CDS 55.0%
CAGTGGCGATGCTGGAAATG+AGG + chr6.4:58334206-58334225 MS.gene05462:CDS 55.0%
CTCATTTCCAGCATCGCCAC+TGG - chr6.4:58334208-58334227 None:intergenic 55.0%
GCCGGATCGAAATCATCTGG+TGG - chr6.4:58334169-58334188 None:intergenic 55.0%
GGTCGTCACCCTTCTCTTCA+TGG + chr6.4:58334394-58334413 MS.gene05462:CDS 55.0%
! GGGAAGGAAGAAGGGGTATG+TGG + chr6.4:58334129-58334148 MS.gene05462:CDS 55.0%
!! GGGATTGGTTGGGGGAAAGA+TGG + chr6.4:58334373-58334392 MS.gene05462:CDS 55.0%


Chromosome Type Strat End Strand Name
chr6.4 gene 58334127 58334577 58334127 ID=MS.gene05462
chr6.4 mRNA 58334127 58334577 58334127 ID=MS.gene05462.t1;Parent=MS.gene05462
chr6.4 exon 58334127 58334415 58334127 ID=MS.gene05462.t1.exon1;Parent=MS.gene05462.t1
chr6.4 CDS 58334127 58334415 58334127 ID=cds.MS.gene05462.t1;Parent=MS.gene05462.t1
chr6.4 exon 58334525 58334577 58334525 ID=MS.gene05462.t1.exon2;Parent=MS.gene05462.t1
chr6.4 CDS 58334525 58334577 58334525 ID=cds.MS.gene05462.t1;Parent=MS.gene05462.t1
Gene Sequence

>MS.gene05462

ATGGGAAGGAAGAAGGGGTATGTGGAGTTCGATGACAACCCACCAGATGATTTCGATCCGGCGAACCCATACAAAGATCCAGTGGCGATGCTGGAAATGAGGGAACACATTGTTCGTGAAAAATGGATTCAGATCGAAAAAGCAAAGATTATTCGTGAAAAGCTTCGTTGGTGTTATAGGATTGAAGGGATTAATCATCATCAGAAATGTCGTCATCTTGTGAAACAGTATCTTGAGTCTACTAGAGGGATTGGTTGGGGGAAAGATGGTCGTCACCCTTCTCTTCATGGTCTGTGTTTTATTCTCTTTTGTTCTCTTTTTGGTGTATAATTGGTTTTTGTTGTGAAATTATGAATTGGGTCTGTGTGATTTTGTCTAAAGTTGTGATTTTTATGTAGTATTGAAGGGTTTGATGTTTTTGTTGATTAAGTGCATGTTTGGATTGATTTGA

Protein sequence

>MS.gene05462.t1

MGRKKGYVEFDDNPPDDFDPANPYKDPVAMLEMREHIVREKWIQIEKAKIIREKLRWCYRIEGINHHQKCRHLVKQYLESTRGIGWGKDGRHPSLHVLKGLMFLLIKCMFGLI