Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98969.t1 | XP_024638642.1 | 93.8 | 128 | 8 | 0 | 1 | 128 | 1 | 128 | 2.20E-57 | 232.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98969.t1 | Q9CX48 | 55.9 | 34 | 15 | 0 | 86 | 119 | 20 | 53 | 5.9e-06 | 52.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98969.t1 | A0A396IFI3 | 93.8 | 128 | 8 | 0 | 1 | 128 | 1 | 128 | 1.6e-57 | 232.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene75592 | MS.gene98969 | PPI |
| MS.gene98969 | MS.gene066824 | PPI |
| MS.gene045256 | MS.gene98969 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98969.t1 | MTR_1g032030 | 91.045 | 67 | 5 | 1 | 18 | 83 | 15 | 81 | 3.62e-22 | 89.0 |
| MS.gene98969.t1 | MTR_1g032370 | 91.045 | 67 | 5 | 1 | 18 | 83 | 15 | 81 | 3.62e-22 | 89.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene98969.t1 | AT5G49400 | 74.803 | 127 | 24 | 2 | 9 | 129 | 16 | 140 | 5.24e-48 | 159 |
Find 51 sgRNAs with CRISPR-Local
Find 59 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GACACATCCGGCCTCAATTT+AGG | 0.222584 | 1.4:+16882325 | None:intergenic |
| ATCAGATTATTCTTCTAGTT+CGG | 0.272097 | 1.4:-16882155 | MS.gene98969:CDS |
| TAGTAAGAAGGAAGAGATTA+AGG | 0.276310 | 1.4:-16882677 | MS.gene98969:CDS |
| ACACATCCGGCCTCAATTTA+GGG | 0.281606 | 1.4:+16882326 | None:intergenic |
| GGCCGCAGTTGCTGCTCTTT+CGG | 0.294122 | 1.4:+16882612 | None:intergenic |
| GTCAAAGAGGAAGCATGATT+TGG | 0.305726 | 1.4:-16882215 | MS.gene98969:CDS |
| TCAGATTATTCTTCTAGTTC+GGG | 0.343157 | 1.4:-16882154 | MS.gene98969:CDS |
| CCATCGATAGTCGACATTGT+AGG | 0.393513 | 1.4:+16882454 | None:intergenic |
| CATGATTTGGGTATTGATAG+TGG | 0.428533 | 1.4:-16882202 | MS.gene98969:CDS |
| TCAAAGAGGAAGCATGATTT+GGG | 0.432855 | 1.4:-16882214 | MS.gene98969:CDS |
| CAGCATTAATTGCTGCGAAA+GGG | 0.433572 | 1.4:-16882651 | MS.gene98969:CDS |
| TGTGTCTGTGGATTATGATT+TGG | 0.452148 | 1.4:-16882308 | MS.gene98969:CDS |
| GCGCGTAATGTTAACGCATA+TGG | 0.458903 | 1.4:-16882589 | MS.gene98969:CDS |
| ATGAGCGGGTTTATATTTCA+AGG | 0.478841 | 1.4:-16882375 | MS.gene98969:CDS |
| GCAGCATTAATTGCTGCGAA+AGG | 0.482444 | 1.4:-16882652 | MS.gene98969:CDS |
| AAATGAGACGGAGGAAGAAG+AGG | 0.488743 | 1.4:-16882102 | MS.gene98969:CDS |
| AGTGTTACCAGGGTGGGCAC+TGG | 0.514214 | 1.4:-16882414 | MS.gene98969:CDS |
| GAATATGTTGAGTAGTAAGA+AGG | 0.518238 | 1.4:-16882689 | None:intergenic |
| CAGTGGATCATCGTCTGCCA+CGG | 0.518690 | 1.4:-16882179 | MS.gene98969:CDS |
| GCAGTGTCAGAAGTGTTACC+AGG | 0.520961 | 1.4:-16882425 | MS.gene98969:CDS |
| TGCTACAGTGGTTGAAAGGA+AGG | 0.521953 | 1.4:-16882485 | MS.gene98969:CDS |
| GCATATGGTCAGAAGGAAGA+AGG | 0.525781 | 1.4:-16882574 | MS.gene98969:CDS |
| TACTGAGAAAGCTGCTACAG+TGG | 0.535042 | 1.4:-16882497 | MS.gene98969:CDS |
| TCATAATCCACAGACACATC+CGG | 0.535556 | 1.4:+16882313 | None:intergenic |
| TTGGGTATTGATAGTGGCAG+TGG | 0.548422 | 1.4:-16882196 | MS.gene98969:CDS |
| AGTGGATCATCGTCTGCCAC+GGG | 0.553302 | 1.4:-16882178 | MS.gene98969:CDS |
| AGAAGGGCCTAGCAGATGGC+AGG | 0.555494 | 1.4:-16882557 | MS.gene98969:CDS |
| TTAAGTTGCTGAGTCCTCGA+TGG | 0.557112 | 1.4:+16882352 | None:intergenic |
| CAGCTCATCGTCTGAATCAG+AGG | 0.559637 | 1.4:-16882128 | MS.gene98969:CDS |
| ATTTCAATGTAAGAATGAGC+GGG | 0.569709 | 1.4:-16882389 | MS.gene98969:CDS |
| AGGAAGAAGGGCCTAGCAGA+TGG | 0.572751 | 1.4:-16882561 | MS.gene98969:CDS |
| AAGCTGCTACAGTGGTTGAA+AGG | 0.575356 | 1.4:-16882489 | MS.gene98969:CDS |
| AGCCGAAAGAGCAGCAACTG+CGG | 0.577539 | 1.4:-16882614 | MS.gene98969:CDS |
| TCGTGATGTGAAGGAGGAGA+AGG | 0.580022 | 1.4:-16882257 | MS.gene98969:CDS |
| TGTTAACGCATATGGTCAGA+AGG | 0.582981 | 1.4:-16882581 | MS.gene98969:CDS |
| TAATAATCATCGTGATGTGA+AGG | 0.585747 | 1.4:-16882266 | MS.gene98969:CDS |
| CATTTCAATGTAAGAATGAG+CGG | 0.604438 | 1.4:-16882390 | MS.gene98969:CDS |
| GTCAGAAGTGTTACCAGGGT+GGG | 0.604446 | 1.4:-16882420 | MS.gene98969:CDS |
| CAGACTAAGAAGAAGAGCAA+AGG | 0.608854 | 1.4:-16882064 | MS.gene98969:CDS |
| CATATGGTCAGAAGGAAGAA+GGG | 0.616780 | 1.4:-16882573 | MS.gene98969:CDS |
| TAATCATCGTGATGTGAAGG+AGG | 0.624901 | 1.4:-16882263 | MS.gene98969:CDS |
| CCTACAATGTCGACTATCGA+TGG | 0.625467 | 1.4:-16882454 | MS.gene98969:CDS |
| AATCAGAGGAAGAAATGAGA+CGG | 0.642262 | 1.4:-16882114 | MS.gene98969:CDS |
| ATTGAGGCCGGATGTGTCTG+TGG | 0.648025 | 1.4:-16882320 | MS.gene98969:CDS |
| GAAATGTCCAGTGCCCACCC+TGG | 0.650305 | 1.4:+16882407 | None:intergenic |
| TGTCAGAAGTGTTACCAGGG+TGG | 0.665235 | 1.4:-16882421 | MS.gene98969:CDS |
| CAGTGTCAGAAGTGTTACCA+GGG | 0.669839 | 1.4:-16882424 | MS.gene98969:CDS |
| CAGAGGAAGAAATGAGACGG+AGG | 0.674783 | 1.4:-16882111 | MS.gene98969:CDS |
| AGAAGAATAATCTGATCCCG+TGG | 0.687932 | 1.4:+16882162 | None:intergenic |
| TTTATATTTCAAGGCCATCG+AGG | 0.711495 | 1.4:-16882366 | MS.gene98969:CDS |
| ATATGCGTTAACATTACGCG+CGG | 0.742150 | 1.4:+16882591 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AACTTAAAAACCCTAAATTG+AGG | - | chr1.4:16882388-16882407 | MS.gene98969:CDS | 25.0% |
| !! | ATCAGATTATTCTTCTAGTT+CGG | - | chr1.4:16882569-16882588 | MS.gene98969:CDS | 25.0% |
| ATTTCAATGTAAGAATGAGC+GGG | - | chr1.4:16882335-16882354 | MS.gene98969:CDS | 30.0% | |
| CATTTCAATGTAAGAATGAG+CGG | - | chr1.4:16882334-16882353 | MS.gene98969:CDS | 30.0% | |
| TAATAATCATCGTGATGTGA+AGG | - | chr1.4:16882458-16882477 | MS.gene98969:CDS | 30.0% | |
| TAGTAAGAAGGAAGAGATTA+AGG | - | chr1.4:16882047-16882066 | MS.gene98969:CDS | 30.0% | |
| !! | TCAGATTATTCTTCTAGTTC+GGG | - | chr1.4:16882570-16882589 | MS.gene98969:CDS | 30.0% |
| AATCAGAGGAAGAAATGAGA+CGG | - | chr1.4:16882610-16882629 | MS.gene98969:CDS | 35.0% | |
| TAAAAACCCTAAATTGAGGC+CGG | - | chr1.4:16882392-16882411 | MS.gene98969:CDS | 35.0% | |
| TGAGTTCGAAAAAGTCAAAG+AGG | - | chr1.4:16882496-16882515 | MS.gene98969:CDS | 35.0% | |
| TTTATATTTCAAGGCCATCG+AGG | - | chr1.4:16882358-16882377 | MS.gene98969:CDS | 35.0% | |
| ! | ATGAGCGGGTTTATATTTCA+AGG | - | chr1.4:16882349-16882368 | MS.gene98969:CDS | 35.0% |
| ! | TCAAAGAGGAAGCATGATTT+GGG | - | chr1.4:16882510-16882529 | MS.gene98969:CDS | 35.0% |
| ! | TGTGTCTGTGGATTATGATT+TGG | - | chr1.4:16882416-16882435 | MS.gene98969:CDS | 35.0% |
| !! | CATGATTTGGGTATTGATAG+TGG | - | chr1.4:16882522-16882541 | MS.gene98969:CDS | 35.0% |
| AGAAGAATAATCTGATCCCG+TGG | + | chr1.4:16882565-16882584 | None:intergenic | 40.0% | |
| AGAAGAGCAAAGGAAAAAGC+AGG | - | chr1.4:16882670-16882689 | MS.gene98969:CDS | 40.0% | |
| ATATGCGTTAACATTACGCG+CGG | + | chr1.4:16882136-16882155 | None:intergenic | 40.0% | |
| CAGACTAAGAAGAAGAGCAA+AGG | - | chr1.4:16882660-16882679 | MS.gene98969:CDS | 40.0% | |
| CAGCATTAATTGCTGCGAAA+GGG | - | chr1.4:16882073-16882092 | MS.gene98969:CDS | 40.0% | |
| CATATGGTCAGAAGGAAGAA+GGG | - | chr1.4:16882151-16882170 | MS.gene98969:CDS | 40.0% | |
| TAATCATCGTGATGTGAAGG+AGG | - | chr1.4:16882461-16882480 | MS.gene98969:CDS | 40.0% | |
| TCATAATCCACAGACACATC+CGG | + | chr1.4:16882414-16882433 | None:intergenic | 40.0% | |
| TGTTAACGCATATGGTCAGA+AGG | - | chr1.4:16882143-16882162 | MS.gene98969:CDS | 40.0% | |
| ! | GTCAAAGAGGAAGCATGATT+TGG | - | chr1.4:16882509-16882528 | MS.gene98969:CDS | 40.0% |
| AAATGAGACGGAGGAAGAAG+AGG | - | chr1.4:16882622-16882641 | MS.gene98969:CDS | 45.0% | |
| AAGCTGCTACAGTGGTTGAA+AGG | - | chr1.4:16882235-16882254 | MS.gene98969:CDS | 45.0% | |
| ACACATCCGGCCTCAATTTA+GGG | + | chr1.4:16882401-16882420 | None:intergenic | 45.0% | |
| AGAGCAAAGGAAAAAGCAGG+AGG | - | chr1.4:16882673-16882692 | MS.gene98969:CDS | 45.0% | |
| CCATCGATAGTCGACATTGT+AGG | + | chr1.4:16882273-16882292 | None:intergenic | 45.0% | |
| GCAGCATTAATTGCTGCGAA+AGG | - | chr1.4:16882072-16882091 | MS.gene98969:CDS | 45.0% | |
| GCATATGGTCAGAAGGAAGA+AGG | - | chr1.4:16882150-16882169 | MS.gene98969:CDS | 45.0% | |
| GCGCGTAATGTTAACGCATA+TGG | - | chr1.4:16882135-16882154 | MS.gene98969:CDS | 45.0% | |
| TACTGAGAAAGCTGCTACAG+TGG | - | chr1.4:16882227-16882246 | MS.gene98969:CDS | 45.0% | |
| TAGCAGATGGCAGGAAAAAC+AGG | - | chr1.4:16882176-16882195 | MS.gene98969:CDS | 45.0% | |
| TGCTACAGTGGTTGAAAGGA+AGG | - | chr1.4:16882239-16882258 | MS.gene98969:CDS | 45.0% | |
| ! | CCTACAATGTCGACTATCGA+TGG | - | chr1.4:16882270-16882289 | MS.gene98969:CDS | 45.0% |
| ! | TGTTTTTCCTGCCATCTGCT+AGG | + | chr1.4:16882177-16882196 | None:intergenic | 45.0% |
| ! | TTAAGTTGCTGAGTCCTCGA+TGG | + | chr1.4:16882375-16882394 | None:intergenic | 45.0% |
| !! | CAGTGTCAGAAGTGTTACCA+GGG | - | chr1.4:16882300-16882319 | MS.gene98969:CDS | 45.0% |
| !! | TTGGGTATTGATAGTGGCAG+TGG | - | chr1.4:16882528-16882547 | MS.gene98969:CDS | 45.0% |
| AGGAAAAACAGGAGGCGAAG+AGG | - | chr1.4:16882187-16882206 | MS.gene98969:CDS | 50.0% | |
| CAGAGGAAGAAATGAGACGG+AGG | - | chr1.4:16882613-16882632 | MS.gene98969:CDS | 50.0% | |
| CAGATGGCAGGAAAAACAGG+AGG | - | chr1.4:16882179-16882198 | MS.gene98969:CDS | 50.0% | |
| CAGCTCATCGTCTGAATCAG+AGG | - | chr1.4:16882596-16882615 | MS.gene98969:CDS | 50.0% | |
| GACACATCCGGCCTCAATTT+AGG | + | chr1.4:16882402-16882421 | None:intergenic | 50.0% | |
| TCGTGATGTGAAGGAGGAGA+AGG | - | chr1.4:16882467-16882486 | MS.gene98969:CDS | 50.0% | |
| !! | GCAGTGTCAGAAGTGTTACC+AGG | - | chr1.4:16882299-16882318 | MS.gene98969:CDS | 50.0% |
| !! | GTCAGAAGTGTTACCAGGGT+GGG | - | chr1.4:16882304-16882323 | MS.gene98969:CDS | 50.0% |
| !! | TGTCAGAAGTGTTACCAGGG+TGG | - | chr1.4:16882303-16882322 | MS.gene98969:CDS | 50.0% |
| AGCCGAAAGAGCAGCAACTG+CGG | - | chr1.4:16882110-16882129 | MS.gene98969:CDS | 55.0% | |
| AGGAAGAAGGGCCTAGCAGA+TGG | - | chr1.4:16882163-16882182 | MS.gene98969:CDS | 55.0% | |
| AGTGGATCATCGTCTGCCAC+GGG | - | chr1.4:16882546-16882565 | MS.gene98969:CDS | 55.0% | |
| ATTGAGGCCGGATGTGTCTG+TGG | - | chr1.4:16882404-16882423 | MS.gene98969:CDS | 55.0% | |
| CAGTGGATCATCGTCTGCCA+CGG | - | chr1.4:16882545-16882564 | MS.gene98969:CDS | 55.0% | |
| AGAAGGGCCTAGCAGATGGC+AGG | - | chr1.4:16882167-16882186 | MS.gene98969:CDS | 60.0% | |
| AGTGTTACCAGGGTGGGCAC+TGG | - | chr1.4:16882310-16882329 | MS.gene98969:CDS | 60.0% | |
| GAAATGTCCAGTGCCCACCC+TGG | + | chr1.4:16882320-16882339 | None:intergenic | 60.0% | |
| GGCCGCAGTTGCTGCTCTTT+CGG | + | chr1.4:16882115-16882134 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 16882039 | 16882707 | 16882039 | ID=MS.gene98969 |
| chr1.4 | mRNA | 16882039 | 16882707 | 16882039 | ID=MS.gene98969.t1;Parent=MS.gene98969 |
| chr1.4 | exon | 16882039 | 16882707 | 16882039 | ID=MS.gene98969.t1.exon1;Parent=MS.gene98969.t1 |
| chr1.4 | CDS | 16882039 | 16882707 | 16882039 | ID=cds.MS.gene98969.t1;Parent=MS.gene98969.t1 |
| Gene Sequence |
| Protein sequence |
