AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene99059

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99059.t1 MTR_4g024020 90.136 294 29 0 1 294 16 309 0.0 553
MS.gene99059.t1 MTR_7g102490 76.271 295 68 2 1 294 16 309 1.39e-165 469
MS.gene99059.t1 MTR_7g102460 71.769 294 81 1 1 294 16 307 3.98e-158 451
MS.gene99059.t1 MTR_7g117405 52.381 294 133 4 1 294 19 305 1.19e-96 293
MS.gene99059.t1 MTR_7g102560 53.691 298 125 7 1 294 16 304 1.42e-96 293
MS.gene99059.t1 MTR_7g102450 51.361 294 123 6 1 294 18 291 1.60e-90 277
MS.gene99059.t1 MTR_5g045970 43.624 298 156 5 1 294 23 312 2.92e-78 246
MS.gene99059.t1 MTR_1g062380 43.390 295 162 4 1 294 23 313 5.04e-78 246
MS.gene99059.t1 MTR_5g045960 45.485 299 147 7 2 294 17 305 7.90e-78 245
MS.gene99059.t1 MTR_7g102520 45.364 302 147 7 1 294 19 310 3.30e-68 221
MS.gene99059.t1 MTR_7g102550 43.243 296 161 5 1 294 16 306 3.83e-64 209
MS.gene99059.t1 MTR_7g071040 38.225 293 175 5 2 293 25 312 1.18e-49 172
MS.gene99059.t1 MTR_7g070930 36.949 295 180 5 2 295 22 311 2.23e-48 168
MS.gene99059.t1 MTR_7g055710 35.548 301 171 8 4 295 21 307 8.69e-48 167
MS.gene99059.t1 MTR_8g066610 32.997 297 192 4 1 294 16 308 3.61e-47 165
MS.gene99059.t1 MTR_7g070910 36.364 297 179 7 2 295 19 308 1.17e-45 161
MS.gene99059.t1 MTR_4g108250 35.197 304 172 10 4 295 21 311 1.61e-45 160
MS.gene99059.t1 MTR_7g070610 36.027 297 175 7 4 295 23 309 1.68e-44 158
MS.gene99059.t1 MTR_7g070740 36.027 297 175 7 4 295 21 307 2.30e-44 157
MS.gene99059.t1 MTR_4g485620 33.904 292 183 5 6 294 1 285 2.36e-43 154
MS.gene99059.t1 MTR_7g070660 34.122 296 177 7 4 295 21 302 3.10e-43 154
MS.gene99059.t1 MTR_7g070780 36.513 304 164 7 4 295 21 307 3.88e-43 154
MS.gene99059.t1 MTR_5g016660 33.000 300 188 7 1 295 17 308 4.21e-43 154
MS.gene99059.t1 MTR_5g039900 35.135 296 185 6 1 295 20 309 4.84e-43 154
MS.gene99059.t1 MTR_7g070800 35.274 292 181 6 4 295 16 299 4.67e-42 151
MS.gene99059.t1 MTR_4g485590 34.354 294 160 5 1 293 16 277 9.73e-42 149
MS.gene99059.t1 MTR_7g070860 35.274 292 181 6 4 295 17 300 1.71e-41 149
MS.gene99059.t1 MTR_6g035295 34.106 302 181 7 1 295 19 309 3.13e-41 149
MS.gene99059.t1 MTR_7g070940 32.203 295 186 6 1 295 16 296 8.90e-40 145
MS.gene99059.t1 MTR_0536s0020 33.559 295 172 7 1 294 25 296 2.53e-39 144
MS.gene99059.t1 MTR_7g070870 34.219 301 164 10 4 295 29 304 2.17e-38 141
MS.gene99059.t1 MTR_7g070810 34.448 299 169 9 4 295 19 297 6.64e-38 140
MS.gene99059.t1 MTR_4g485630 33.333 300 175 10 1 293 16 297 1.70e-37 139
MS.gene99059.t1 MTR_4g117980 31.544 298 182 8 1 293 18 298 4.57e-37 138
MS.gene99059.t1 MTR_4g117950 31.922 307 177 9 1 299 13 295 1.91e-36 136
MS.gene99059.t1 MTR_4g117890 31.757 296 176 8 1 294 20 291 2.48e-36 135
MS.gene99059.t1 MTR_4g117960 33.106 293 180 6 2 293 19 296 4.27e-36 135
MS.gene99059.t1 MTR_4g031800 30.169 295 188 6 2 294 17 295 5.66e-36 135
MS.gene99059.t1 MTR_4g118000 31.987 297 169 8 2 294 20 287 7.25e-36 134
MS.gene99059.t1 MTR_4g485640 33.904 292 172 7 4 295 219 489 1.13e-35 135
MS.gene99059.t1 MTR_0536s0010 30.847 295 178 8 1 294 28 297 7.48e-35 132
MS.gene99059.t1 MTR_5g040030 34.951 309 167 10 2 295 22 311 1.71e-34 131
MS.gene99059.t1 MTR_1g040310 32.248 307 177 11 1 295 22 309 4.22e-33 127
MS.gene99059.t1 MTR_6g035265 31.650 297 174 8 1 293 18 289 4.93e-33 127
MS.gene99059.t1 MTR_2g034990 29.293 297 192 7 1 294 18 299 8.38e-32 123
MS.gene99059.t1 MTR_6g035185 33.557 298 174 8 2 293 19 298 2.00e-31 122
MS.gene99059.t1 MTR_2g035020 30.068 296 189 7 1 293 18 298 2.20e-31 122
MS.gene99059.t1 MTR_8g044140 31.930 285 169 9 21 295 3 272 3.16e-31 121
MS.gene99059.t1 MTR_7g012120 29.054 296 193 6 1 295 23 302 2.09e-28 114
MS.gene99059.t1 MTR_8g032950 31.000 300 180 9 1 294 19 297 3.23e-26 108
MS.gene99059.t1 MTR_7g071050 29.795 292 154 8 4 295 23 263 4.58e-26 107
MS.gene99059.t1 MTR_5g029800 28.716 296 189 8 1 293 18 294 6.85e-26 107
MS.gene99059.t1 MTR_5g067170 29.730 296 186 9 2 293 19 296 1.38e-24 103
MS.gene99059.t1 MTR_5g087620 26.384 307 206 8 1 294 17 316 1.29e-21 94.7
MS.gene99059.t1 MTR_4g094220 26.625 323 189 11 2 295 15 318 7.94e-21 92.4
MS.gene99059.t1 MTR_7g113320 34.266 143 93 1 70 212 11 152 4.39e-20 86.7
MS.gene99059.t1 MTR_2g059240 31.046 306 181 13 2 294 21 309 8.31e-20 89.4
MS.gene99059.t1 MTR_5g464760 26.367 311 202 7 2 295 20 320 4.43e-18 84.3
MS.gene99059.t1 MTR_0036s0220 25.161 310 205 8 2 295 20 318 6.38e-18 84.0
MS.gene99059.t1 MTR_2g008210 27.244 312 193 13 2 295 19 314 2.99e-17 82.0
MS.gene99059.t1 MTR_4g117970 28.774 212 136 6 66 276 55 252 5.51e-17 80.9
MS.gene99059.t1 MTR_5g464810 26.144 306 207 8 2 295 20 318 2.88e-16 79.3
MS.gene99059.t1 MTR_5g064240 26.144 306 207 8 2 295 20 318 2.88e-16 79.3
MS.gene99059.t1 MTR_7g470610 30.851 188 100 6 113 295 2 164 3.09e-16 77.8
MS.gene99059.t1 MTR_7g070583 30.851 188 100 6 113 295 2 164 3.09e-16 77.8
MS.gene99059.t1 MTR_0009s0240 25.874 286 184 10 2 281 15 278 9.16e-16 77.8
MS.gene99059.t1 MTR_2g035040 32.105 190 116 5 107 293 24 203 1.64e-15 76.6
MS.gene99059.t1 MTR_8g071070 26.230 305 197 11 2 295 15 302 4.14e-15 75.9
MS.gene99059.t1 MTR_2g098430 24.342 304 202 9 1 294 13 298 4.75e-15 75.5
MS.gene99059.t1 MTR_6g014220 25.846 325 184 13 3 295 19 318 9.18e-15 74.7
MS.gene99059.t1 MTR_3g008010 25.397 315 200 13 2 295 18 318 9.38e-15 74.7
MS.gene99059.t1 MTR_0536s0030 27.692 195 115 5 24 215 9 180 1.37e-14 73.6
MS.gene99059.t1 MTR_6g014020 24.765 319 199 10 2 295 18 320 2.49e-14 73.6
MS.gene99059.t1 MTR_6g013440 24.138 319 201 11 2 295 19 321 4.72e-14 72.4
MS.gene99059.t1 MTR_7g470590 38.889 108 62 3 4 110 21 125 1.68e-13 66.6
MS.gene99059.t1 MTR_7g070570 38.889 108 62 3 4 110 21 125 1.68e-13 66.6
MS.gene99059.t1 MTR_7g011470 26.910 301 195 11 3 295 19 302 2.22e-13 70.5
MS.gene99059.t1 MTR_7g039570 32.065 184 106 6 2 177 22 194 2.99e-13 68.6
MS.gene99059.t1 MTR_6g014290 22.857 315 208 10 2 295 18 318 4.27e-13 69.7
MS.gene99059.t1 MTR_7g070760 42.157 102 55 3 196 295 23 122 5.03e-13 67.4
MS.gene99059.t1 MTR_8g466240 25.581 301 182 12 2 290 16 286 8.30e-13 68.9
MS.gene99059.t1 MTR_7g080940 27.124 306 179 13 3 295 20 294 1.18e-12 68.2
MS.gene99059.t1 MTR_6g038200 27.575 301 186 13 2 294 19 295 1.79e-12 67.8
MS.gene99059.t1 MTR_7g011470 26.557 305 195 12 3 295 19 306 4.55e-12 66.6
MS.gene99059.t1 MTR_6g014187 25.243 309 208 12 3 295 19 320 2.09e-11 64.7
MS.gene99059.t1 MTR_8g056710 34.074 135 59 4 121 250 22 131 2.74e-11 62.0
MS.gene99059.t1 MTR_6g014270 24.149 323 196 11 3 295 16 319 2.75e-11 64.3
MS.gene99059.t1 MTR_6g013960 24.611 321 201 10 2 297 18 322 3.42e-11 63.9
MS.gene99059.t1 MTR_3g069690 23.473 311 180 8 3 295 17 287 5.32e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99059.t1 AT2G36770 48.311 296 145 5 1 294 21 310 1.38e-85 265
MS.gene99059.t1 AT2G36750 45.918 294 154 3 1 294 17 305 3.26e-83 259
MS.gene99059.t1 AT2G36800 46.959 296 150 4 1 294 19 309 3.95e-82 256
MS.gene99059.t1 AT2G36780 46.959 296 149 5 1 294 21 310 9.17e-82 255
MS.gene99059.t1 AT2G36760 45.763 295 154 5 1 294 21 310 1.18e-80 253
MS.gene99059.t1 AT2G36790 46.341 287 146 5 1 285 20 300 7.00e-80 250
MS.gene99059.t1 AT3G53150 43.729 295 162 4 1 294 29 320 1.27e-75 240
MS.gene99059.t1 AT3G53160 42.193 301 156 6 1 294 15 304 1.49e-75 239
MS.gene99059.t1 AT4G34131 34.437 302 182 7 1 294 17 310 1.26e-48 169
MS.gene99059.t1 AT2G15480 33.993 303 184 7 1 295 17 311 3.93e-47 165
MS.gene99059.t1 AT2G15480 33.993 303 184 7 1 295 17 311 4.26e-47 165
MS.gene99059.t1 AT2G15490 35.314 303 180 8 1 295 14 308 5.24e-45 159
MS.gene99059.t1 AT2G15490 35.314 303 180 8 1 295 14 308 5.24e-45 159
MS.gene99059.t1 AT4G34135 33.775 302 184 7 1 294 18 311 8.89e-45 155
MS.gene99059.t1 AT4G34138 35.314 303 175 10 1 293 18 309 1.84e-44 157
MS.gene99059.t1 AT4G34135 33.887 301 183 7 1 293 18 310 2.60e-43 154
MS.gene99059.t1 AT2G16890 30.719 306 183 12 1 295 16 303 4.39e-32 121
MS.gene99059.t1 AT2G16890 30.719 306 183 12 1 295 16 303 4.39e-32 121
MS.gene99059.t1 AT2G16890 30.719 306 183 12 1 295 16 303 3.54e-31 121
MS.gene99059.t1 AT2G16890 30.719 306 183 12 1 295 16 303 8.45e-31 120
MS.gene99059.t1 AT5G14860 32.075 318 169 14 1 294 15 309 3.32e-27 110
MS.gene99059.t1 AT1G10400 27.010 311 188 12 1 294 14 302 5.95e-23 98.6
MS.gene99059.t1 AT1G73880 28.571 301 187 12 2 294 22 302 2.05e-22 97.1
MS.gene99059.t1 AT2G15490 34.254 181 107 6 1 173 14 190 5.86e-21 90.5
MS.gene99059.t1 AT1G22380 28.571 322 180 12 2 295 21 320 4.99e-20 90.1
MS.gene99059.t1 AT1G22370 27.974 311 191 14 2 295 21 315 2.98e-17 82.0
MS.gene99059.t1 AT1G22360 26.367 311 201 11 2 295 18 317 1.24e-16 80.1
MS.gene99059.t1 AT1G22360 26.367 311 201 11 2 295 18 317 1.46e-16 80.1
MS.gene99059.t1 AT1G22400 27.586 319 188 12 2 295 21 321 1.92e-16 79.7
MS.gene99059.t1 AT1G51210 27.458 295 188 10 2 293 28 299 2.92e-16 79.0
MS.gene99059.t1 AT3G46650 24.161 298 178 8 2 295 18 271 4.25e-11 63.5

Find 54 sgRNAs with CRISPR-Local

Find 120 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTACATGCCATTAATATTA+TGG 0.159784 4.1:+74637660 MS.gene99059:CDS
CATGATTTCCATAATATTAA+TGG 0.176093 4.1:-74637668 None:intergenic
CTAGCTATGTGGATTGTATA+TGG 0.276076 4.1:-74637571 None:intergenic
TTGCTGCAGTAAGTTGCTTC+TGG 0.276341 4.1:+74637620 MS.gene99059:CDS
AACTTTCCATGTAAAGAGTT+TGG 0.286014 4.1:+74637394 MS.gene99059:CDS
ACTGCAGCAAAAGAAATTCT+TGG 0.311271 4.1:-74637607 None:intergenic
ACTTAGTAACATTGATGAAT+TGG 0.326831 4.1:+74637939 MS.gene99059:CDS
AGATTAGAAATGACAAAGTT+TGG 0.331276 4.1:+74637899 MS.gene99059:CDS
GGATATACTTGAAGCTGAAA+TGG 0.374963 4.1:+74637810 MS.gene99059:CDS
TCAAGATTCTCACACCCTTC+TGG 0.375312 4.1:-74637421 None:intergenic
ATCCTCATTAAATTGTTTCA+AGG 0.376454 4.1:-74637791 None:intergenic
GTAGCACGTCATGTTGAATC+TGG 0.379621 4.1:+74637346 MS.gene99059:CDS
GAAGCTGAAATGGGTAGTTA+TGG 0.395187 4.1:+74637820 MS.gene99059:CDS
AATGACAAAGTTTGGTGTAT+AGG 0.398133 4.1:+74637907 MS.gene99059:CDS
TTCAAGTCCCTTACATATGT+TGG 0.399868 4.1:-74637874 None:intergenic
TAACATTGATGAATTGGATA+AGG 0.404858 4.1:+74637945 MS.gene99059:CDS
ACCCTAGTCCTGTTTGTGCT+AGG 0.406283 4.1:-74637750 None:intergenic
GAGAATCTTGACATGCTGCC+TGG 0.426628 4.1:+74637433 MS.gene99059:CDS
ACTAGGGTCAACAAAGGTTA+TGG 0.429662 4.1:+74637765 MS.gene99059:CDS
GATGGACACTGCAAAACTAT+TGG 0.435532 4.1:+74637255 MS.gene99059:CDS
AAGCTGAAATGGGTAGTTAT+GGG 0.442213 4.1:+74637821 MS.gene99059:CDS
CATTAATATTATGGAAATCA+TGG 0.448368 4.1:+74637669 MS.gene99059:CDS
CCCTAGTCCTGTTTGTGCTA+GGG 0.460150 4.1:-74637749 None:intergenic
AGTTAGAGCCAACATATGTA+AGG 0.466189 4.1:+74637866 MS.gene99059:CDS
TGATACATTGAAGAAGTTTG+AGG 0.472014 4.1:-74637467 None:intergenic
TCAGAGATAATACAAGTTGC+TGG 0.475164 4.1:-74637538 None:intergenic
ATTGAAGAAGTTTGAGGCTA+GGG 0.477454 4.1:-74637461 None:intergenic
TCAATGTTACTAAGTGATAC+AGG 0.479291 4.1:-74637931 None:intergenic
TATTGAACTTTCTAGCTATG+TGG 0.480031 4.1:-74637582 None:intergenic
GATATACTTGAAGCTGAAAT+GGG 0.488641 4.1:+74637811 MS.gene99059:CDS
GAAATGACCCTAGCACAAAC+AGG 0.490631 4.1:+74637742 MS.gene99059:CDS
GAAGTGGCTAAATTCTCATA+AGG 0.494972 4.1:+74638017 MS.gene99059:CDS
GGTAATCCAAACTCTTTACA+TGG 0.498013 4.1:-74637400 None:intergenic
CATTGAAGAAGTTTGAGGCT+AGG 0.499517 4.1:-74637462 None:intergenic
AGCCTTGAAACAATTTAATG+AGG 0.509352 4.1:+74637789 MS.gene99059:CDS
TTTGAGGCTAGGGCAAGTCC+AGG 0.535087 4.1:-74637451 None:intergenic
TTAGATTACATAAACTTCCA+AGG 0.537093 4.1:-74638062 None:intergenic
CCACCACAGTTGATAGAGCT+AGG 0.542078 4.1:+74638087 MS.gene99059:CDS
AGTGTTATATATGCAAGCCT+TGG 0.542503 4.1:+74638045 MS.gene99059:CDS
ACCCTAGCACAAACAGGACT+AGG 0.553952 4.1:+74637748 MS.gene99059:CDS
TGTCCATCATGGGGATCATG+TGG 0.573417 4.1:-74637240 None:intergenic
AAAGAGTTTGGATTACCAGA+AGG 0.576750 4.1:+74637406 MS.gene99059:CDS
AAGAGTTTGGATTACCAGAA+GGG 0.580939 4.1:+74637407 MS.gene99059:CDS
AGGCCACATGATCCCCATGA+TGG 0.587737 4.1:+74637237 MS.gene99059:CDS
CCCTAGCACAAACAGGACTA+GGG 0.604808 4.1:+74637749 MS.gene99059:CDS
GTTAGAGCCAACATATGTAA+GGG 0.620691 4.1:+74637867 MS.gene99059:CDS
CACCACAGTTGATAGAGCTA+GGG 0.621125 4.1:+74638088 MS.gene99059:CDS
CCTAGCTCTATCAACTGTGG+TGG 0.636929 4.1:-74638087 None:intergenic
ATGAATGGAAGCACTTGAAG+TGG 0.663535 4.1:+74638001 MS.gene99059:CDS
GAATTGGATAAGGTTCAAAG+AGG 0.679282 4.1:+74637955 MS.gene99059:CDS
TTGTTTCCAATGATGGCACA+AGG 0.685173 4.1:+74637217 None:intergenic
AACCCTAGCTCTATCAACTG+TGG 0.685229 4.1:-74638090 None:intergenic
GAGATAATACAAGTTGCTGG+TGG 0.692094 4.1:-74637535 None:intergenic
AACAGGACTAGGGTCAACAA+AGG 0.692624 4.1:+74637759 MS.gene99059:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATGTAAAGAAAGAAGAAAA+AGG + chr4.1:74638535-74638554 MS.gene99059:intron 20.0%
!! ATAATACATGTTTCTTCATA+TGG - chr4.1:74638676-74638695 None:intergenic 20.0%
!! CATGATTTCCATAATATTAA+TGG - chr4.1:74637671-74637690 None:intergenic 20.0%
!! CATTAATATTATGGAAATCA+TGG + chr4.1:74637669-74637688 MS.gene99059:CDS 20.0%
!! TTTACATGCCATTAATATTA+TGG + chr4.1:74637660-74637679 MS.gene99059:CDS 20.0%
!!! AACAAAAAGACCTTTTATTT+GGG + chr4.1:74638125-74638144 MS.gene99059:intron 20.0%
!!! GTACTATTATTTTTAAATGC+AGG + chr4.1:74638742-74638761 MS.gene99059:intron 20.0%
!!! TTTTTATCTTCATGATATCT+TGG - chr4.1:74638638-74638657 None:intergenic 20.0%
! AAAAATTGATCAGCAAAAAG+TGG - chr4.1:74638363-74638382 None:intergenic 25.0%
! ACTTAGTAACATTGATGAAT+TGG + chr4.1:74637939-74637958 MS.gene99059:CDS 25.0%
! AGATTAGAAATGACAAAGTT+TGG + chr4.1:74637899-74637918 MS.gene99059:CDS 25.0%
! AGTTAGAAGAGTTGAAAAAA+TGG + chr4.1:74638166-74638185 MS.gene99059:intron 25.0%
! ATCCTCATTAAATTGTTTCA+AGG - chr4.1:74637794-74637813 None:intergenic 25.0%
! GTGATAAAATCAATAACTGA+GGG - chr4.1:74638254-74638273 None:intergenic 25.0%
! TAACATTGATGAATTGGATA+AGG + chr4.1:74637945-74637964 MS.gene99059:CDS 25.0%
! TCTCTAATAACCCAAATAAA+AGG - chr4.1:74638138-74638157 None:intergenic 25.0%
! TGATAAAATCAATAACTGAG+GGG - chr4.1:74638253-74638272 None:intergenic 25.0%
! TGTGATAAAATCAATAACTG+AGG - chr4.1:74638255-74638274 None:intergenic 25.0%
! TTAGATTACATAAACTTCCA+AGG - chr4.1:74638065-74638084 None:intergenic 25.0%
!! ATATCTTTTCAACTTCTTGT+TGG - chr4.1:74637501-74637520 None:intergenic 25.0%
!! CAACAAAAAGACCTTTTATT+TGG + chr4.1:74638124-74638143 MS.gene99059:intron 25.0%
!! GATAATCACAAATTCTTTTG+AGG + chr4.1:74637843-74637862 MS.gene99059:CDS 25.0%
!! TTTATTTGGGTTATTAGAGA+GGG + chr4.1:74638138-74638157 MS.gene99059:intron 25.0%
!!! ACAAAGTTTTAGTTGATGAA+TGG + chr4.1:74637986-74638005 MS.gene99059:CDS 25.0%
!!! TGAAAGTTGGTGTAAAAATT+GGG + chr4.1:74638409-74638428 MS.gene99059:intron 25.0%
!!! TTGAAAGTTGGTGTAAAAAT+TGG + chr4.1:74638408-74638427 MS.gene99059:intron 25.0%
!!! TTTTATTTGGGTTATTAGAG+AGG + chr4.1:74638137-74638156 MS.gene99059:intron 25.0%
AACTTTCCATGTAAAGAGTT+TGG + chr4.1:74637394-74637413 MS.gene99059:CDS 30.0%
AAGAAAGAAGATGTTGAAAG+AGG + chr4.1:74638483-74638502 MS.gene99059:intron 30.0%
AGCCTTGAAACAATTTAATG+AGG + chr4.1:74637789-74637808 MS.gene99059:CDS 30.0%
GATATACTTGAAGCTGAAAT+GGG + chr4.1:74637811-74637830 MS.gene99059:CDS 30.0%
GTTGTGCAAATATTGAAAGT+TGG + chr4.1:74638396-74638415 MS.gene99059:intron 30.0%
TCAATGTTACTAAGTGATAC+AGG - chr4.1:74637934-74637953 None:intergenic 30.0%
TGATACATTGAAGAAGTTTG+AGG - chr4.1:74637470-74637489 None:intergenic 30.0%
! ATTTTATCACATCCTGCAAT+TGG + chr4.1:74638264-74638283 MS.gene99059:intron 30.0%
!! AAGAGGAATAGAAGTGTTAA+TGG + chr4.1:74638500-74638519 MS.gene99059:intron 30.0%
!! AATGACAAAGTTTGGTGTAT+AGG + chr4.1:74637907-74637926 MS.gene99059:CDS 30.0%
!! AGAGTTGAAAAAATGGATTG+AGG + chr4.1:74638173-74638192 MS.gene99059:intron 30.0%
!! GAAAGTTGGTGTAAAAATTG+GGG + chr4.1:74638410-74638429 MS.gene99059:intron 30.0%
!! TATTGAACTTTCTAGCTATG+TGG - chr4.1:74637585-74637604 None:intergenic 30.0%
!!! TGAACCTAGATGCATTTTTT+GGG - chr4.1:74637318-74637337 None:intergenic 30.0%
AAAAAGGCTGTAGAAAAAGG+TGG + chr4.1:74638582-74638601 MS.gene99059:intron 35.0%
AAAGAGTTTGGATTACCAGA+AGG + chr4.1:74637406-74637425 MS.gene99059:CDS 35.0%
AAGAGTTTGGATTACCAGAA+GGG + chr4.1:74637407-74637426 MS.gene99059:CDS 35.0%
AAGCTGAAATGGGTAGTTAT+GGG + chr4.1:74637821-74637840 MS.gene99059:CDS 35.0%
ACTGCAGCAAAAGAAATTCT+TGG - chr4.1:74637610-74637629 None:intergenic 35.0%
AGTAGAATTCCAACCACAAT+GGG - chr4.1:74638301-74638320 None:intergenic 35.0%
AGTGTTATATATGCAAGCCT+TGG + chr4.1:74638045-74638064 MS.gene99059:CDS 35.0%
AGTTAGAGCCAACATATGTA+AGG + chr4.1:74637866-74637885 MS.gene99059:CDS 35.0%
GAAGTGGCTAAATTCTCATA+AGG + chr4.1:74638017-74638036 MS.gene99059:CDS 35.0%
GAATTGGATAAGGTTCAAAG+AGG + chr4.1:74637955-74637974 MS.gene99059:CDS 35.0%
GCTAAAAAGGCTGTAGAAAA+AGG + chr4.1:74638579-74638598 MS.gene99059:intron 35.0%
GGATATACTTGAAGCTGAAA+TGG + chr4.1:74637810-74637829 MS.gene99059:CDS 35.0%
GGTAATCCAAACTCTTTACA+TGG - chr4.1:74637403-74637422 None:intergenic 35.0%
GTTAGAGCCAACATATGTAA+GGG + chr4.1:74637867-74637886 MS.gene99059:CDS 35.0%
TAGTAGAATTCCAACCACAA+TGG - chr4.1:74638302-74638321 None:intergenic 35.0%
TCAGAGATAATACAAGTTGC+TGG - chr4.1:74637541-74637560 None:intergenic 35.0%
TTCAAGTCCCTTACATATGT+TGG - chr4.1:74637877-74637896 None:intergenic 35.0%
TTGTGGTTGGAATTCTACTA+TGG + chr4.1:74638302-74638321 MS.gene99059:intron 35.0%
! AGGGTCATTTCAATTTTGTC+AGG - chr4.1:74637733-74637752 None:intergenic 35.0%
! ATTGAAGAAGTTTGAGGCTA+GGG - chr4.1:74637464-74637483 None:intergenic 35.0%
! CTAGCTATGTGGATTGTATA+TGG - chr4.1:74637574-74637593 None:intergenic 35.0%
! GGGTCATTTCAATTTTGTCA+GGG - chr4.1:74637732-74637751 None:intergenic 35.0%
! TCAATTTTGTCAGGGATATC+AGG - chr4.1:74637724-74637743 None:intergenic 35.0%
!! AAAAAATGGATTGAGGAGAG+TGG + chr4.1:74638180-74638199 MS.gene99059:intron 35.0%
!!! GAACCTAGATGCATTTTTTG+GGG - chr4.1:74637317-74637336 None:intergenic 35.0%
!!! GTGAACCTAGATGCATTTTT+TGG - chr4.1:74637319-74637338 None:intergenic 35.0%
AAAAGTGGCCATGTAACCAT+TGG - chr4.1:74638348-74638367 None:intergenic 40.0%
ACTAGGGTCAACAAAGGTTA+TGG + chr4.1:74637765-74637784 MS.gene99059:CDS 40.0%
ACTATGGAAGCAATATGTGC+TGG + chr4.1:74638318-74638337 MS.gene99059:intron 40.0%
AGGCCTAGTAATTAAGGGTT+GGG + chr4.1:74638227-74638246 MS.gene99059:intron 40.0%
CAACCCCAAAAAATGCATCT+AGG + chr4.1:74637311-74637330 MS.gene99059:CDS 40.0%
GAAGCTGAAATGGGTAGTTA+TGG + chr4.1:74637820-74637839 MS.gene99059:CDS 40.0%
GAGATAATACAAGTTGCTGG+TGG - chr4.1:74637538-74637557 None:intergenic 40.0%
GATGGACACTGCAAAACTAT+TGG + chr4.1:74637255-74637274 MS.gene99059:CDS 40.0%
GGAAGAAAAAAGTGGTGTGT+TGG + chr4.1:74638458-74638477 MS.gene99059:intron 40.0%
GGATTTGAGGGAAGAATCAA+TGG + chr4.1:74638201-74638220 MS.gene99059:intron 40.0%
GGTAGAGGCCTAGTAATTAA+GGG + chr4.1:74638222-74638241 MS.gene99059:intron 40.0%
GGTGAAGAGTCCTATTCAAT+GGG + chr4.1:74638431-74638450 MS.gene99059:intron 40.0%
GTCAAAAATCCTCCAATTGC+AGG - chr4.1:74638279-74638298 None:intergenic 40.0%
GTGAAGAGTCCTATTCAATG+GGG + chr4.1:74638432-74638451 MS.gene99059:intron 40.0%
TGGTAGAGGCCTAGTAATTA+AGG + chr4.1:74638221-74638240 MS.gene99059:intron 40.0%
TTATCACATCCTGCAATTGG+AGG + chr4.1:74638267-74638286 MS.gene99059:intron 40.0%
! AATATGTGCTGGTGTACCAA+TGG + chr4.1:74638329-74638348 MS.gene99059:intron 40.0%
! AGTTTTGCAGTGTCCATCAT+GGG - chr4.1:74637253-74637272 None:intergenic 40.0%
! ATTGAGGAGAGTGGATTTGA+GGG + chr4.1:74638189-74638208 MS.gene99059:intron 40.0%
! CATTGAAGAAGTTTGAGGCT+AGG - chr4.1:74637465-74637484 None:intergenic 40.0%
! GATTTTTGACCCATTGTGGT+TGG + chr4.1:74638289-74638308 MS.gene99059:intron 40.0%
!! ATGAATGGAAGCACTTGAAG+TGG + chr4.1:74638001-74638020 MS.gene99059:CDS 40.0%
!! TAGTTTTGCAGTGTCCATCA+TGG - chr4.1:74637254-74637273 None:intergenic 40.0%
AACAGGACTAGGGTCAACAA+AGG + chr4.1:74637759-74637778 MS.gene99059:CDS 45.0%
CTGGTGTACCAATGGTTACA+TGG + chr4.1:74638337-74638356 MS.gene99059:intron 45.0%
GAAATGACCCTAGCACAAAC+AGG + chr4.1:74637742-74637761 MS.gene99059:CDS 45.0%
GAGGCCTAGTAATTAAGGGT+TGG + chr4.1:74638226-74638245 MS.gene99059:intron 45.0%
GAGGGAAGAATCAATGGTAG+AGG + chr4.1:74638207-74638226 MS.gene99059:intron 45.0%
GGGTGAAGAGTCCTATTCAA+TGG + chr4.1:74638430-74638449 MS.gene99059:intron 45.0%
GTAGCACGTCATGTTGAATC+TGG + chr4.1:74637346-74637365 MS.gene99059:CDS 45.0%
TCAAGATTCTCACACCCTTC+TGG - chr4.1:74637424-74637443 None:intergenic 45.0%
TCTTCCTCACCCCATTGAAT+AGG - chr4.1:74638444-74638463 None:intergenic 45.0%
TGGGGTGAGGAAGAAAAAAG+TGG + chr4.1:74638450-74638469 MS.gene99059:intron 45.0%
TTGCTGCAGTAAGTTGCTTC+TGG + chr4.1:74637620-74637639 MS.gene99059:CDS 45.0%
! GATTGAGGAGAGTGGATTTG+AGG + chr4.1:74638188-74638207 MS.gene99059:intron 45.0%
! GCTTGCTGAGATGGCTAAAA+AGG + chr4.1:74638566-74638585 MS.gene99059:intron 45.0%
! GGAGGATTTTTGACCCATTG+TGG + chr4.1:74638285-74638304 MS.gene99059:intron 45.0%
! GTTTTGCAGTGTCCATCATG+GGG - chr4.1:74637252-74637271 None:intergenic 45.0%
!! AACCCTAGCTCTATCAACTG+TGG - chr4.1:74638093-74638112 None:intergenic 45.0%
!! CACCACAGTTGATAGAGCTA+GGG + chr4.1:74638088-74638107 MS.gene99059:CDS 45.0%
!! GATTAGAGAGCTTGCTGAGA+TGG + chr4.1:74638557-74638576 MS.gene99059:intron 45.0%
ACCCTAGCACAAACAGGACT+AGG + chr4.1:74637748-74637767 MS.gene99059:CDS 50.0%
ACCCTAGTCCTGTTTGTGCT+AGG - chr4.1:74637753-74637772 None:intergenic 50.0%
CCCTAGCACAAACAGGACTA+GGG + chr4.1:74637749-74637768 MS.gene99059:CDS 50.0%
CCCTAGTCCTGTTTGTGCTA+GGG - chr4.1:74637752-74637771 None:intergenic 50.0%
GAGAATCTTGACATGCTGCC+TGG + chr4.1:74637433-74637452 MS.gene99059:CDS 50.0%
GAGTCCTATTCAATGGGGTG+AGG + chr4.1:74638437-74638456 MS.gene99059:intron 50.0%
GGGCCCAACCCTTAATTACT+AGG - chr4.1:74638233-74638252 None:intergenic 50.0%
TGTCCATCATGGGGATCATG+TGG - chr4.1:74637243-74637262 None:intergenic 50.0%
!! CCACCACAGTTGATAGAGCT+AGG + chr4.1:74638087-74638106 MS.gene99059:CDS 50.0%
!! CCTAGCTCTATCAACTGTGG+TGG - chr4.1:74638090-74638109 None:intergenic 50.0%
AGGCCACATGATCCCCATGA+TGG + chr4.1:74637237-74637256 MS.gene99059:CDS 55.0%
! TTTGAGGCTAGGGCAAGTCC+AGG - chr4.1:74637454-74637473 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 74637229 74638782 74637229 ID=MS.gene99059
chr4.1 mRNA 74637229 74638782 74637229 ID=MS.gene99059.t1;Parent=MS.gene99059
chr4.1 exon 74637229 74638109 74637229 ID=MS.gene99059.t1.exon1;Parent=MS.gene99059.t1
chr4.1 CDS 74637229 74638109 74637229 ID=cds.MS.gene99059.t1;Parent=MS.gene99059.t1
chr4.1 exon 74638764 74638782 74638764 ID=MS.gene99059.t1.exon2;Parent=MS.gene99059.t1
chr4.1 CDS 74638764 74638782 74638764 ID=cds.MS.gene99059.t1;Parent=MS.gene99059.t1
Gene Sequence

>MS.gene99059

ATGGCACAAGGCCACATGATCCCCATGATGGACACTGCAAAACTATTGGCACATCATGAAAATGTTGTAGTTACAATAGTAACAACCCCAAAAAATGCATCTAGGTTCACATCATTTGTAGCACGTCATGTTGAATCTGGTCTTCACATTCAATTCATCAAACTCAACTTTCCATGTAAAGAGTTTGGATTACCAGAAGGGTGTGAGAATCTTGACATGCTGCCTGGACTTGCCCTAGCCTCAAACTTCTTCAATGTATCAAAACTTCTCCAACAAGAAGTTGAAAAGATATTTGAAGAGTTAACACCACCAGCAACTTGTATTATCTCTGATACGATTTTGCCATATACAATCCACATAGCTAGAAAGTTCAATATTCCAAGAATTTCTTTTGCTGCAGTAAGTTGCTTCTGGCTCTACAATATACATAATTTACATGCCATTAATATTATGGAAATCATGGCTAATAAAGAATCTGAATACTTTGATTTGCCTGATATCCCTGACAAAATTGAAATGACCCTAGCACAAACAGGACTAGGGTCAACAAAGGTTATGGAAGCCTTGAAACAATTTAATGAGGATATACTTGAAGCTGAAATGGGTAGTTATGGGATAATCACAAATTCTTTTGAGGAGTTAGAGCCAACATATGTAAGGGACTTGAAAAAGATTAGAAATGACAAAGTTTGGTGTATAGGACCTGTATCACTTAGTAACATTGATGAATTGGATAAGGTTCAAAGAGGTAATAGTAACAAAGTTTTAGTTGATGAATGGAAGCACTTGAAGTGGCTAAATTCTCATAAGGATGAGAGTGTTATATATGCAAGCCTTGGAAGTTTATGTAATCTAACACCACCACAGTTGATAGAGCTAGGGTTAGCATTAGAAGCAACAAAAAGACCTTTTATTTGGGTTATTAGAGAGGGAAATCAGTTAGAAGAGTTGAAAAAATGGATTGAGGAGAGTGGATTTGAGGGAAGAATCAATGGTAGAGGCCTAGTAATTAAGGGTTGGGCCCCTCAGTTATTGATTTTATCACATCCTGCAATTGGAGGATTTTTGACCCATTGTGGTTGGAATTCTACTATGGAAGCAATATGTGCTGGTGTACCAATGGTTACATGGCCACTTTTTGCTGATCAATTTTTTAATGAATGTTTAGTTGTGCAAATATTGAAAGTTGGTGTAAAAATTGGGGTGAAGAGTCCTATTCAATGGGGTGAGGAAGAAAAAAGTGGTGTGTTGGTGAAGAAAGAAGATGTTGAAAGAGGAATAGAAGTGTTAATGGATGAGACAGATGAATGTAAAGAAAGAAGAAAAAGGATTAGAGAGCTTGCTGAGATGGCTAAAAAGGCTGTAGAAAAAGGTGGATCTTCTCACTCTAATGTTGCTTTGTTCATCCAAGATATCATGAAGATAAAAAATAAAGTTACAAAAACCATATGAAGAAACATGTATTATATGTTTAATAGTTGTACTATTATTATTTTGTCAAAAAAAAAAATTTGTACTATTATTTTTAAATGCAGGTATCTCTTCCTTTATTAA
Protein sequence

>MS.gene99059.t1

MAQGHMIPMMDTAKLLAHHENVVVTIVTTPKNASRFTSFVARHVESGLHIQFIKLNFPCKEFGLPEGCENLDMLPGLALASNFFNVSKLLQQEVEKIFEELTPPATCIISDTILPYTIHIARKFNIPRISFAAVSCFWLYNIHNLHAINIMEIMANKESEYFDLPDIPDKIEMTLAQTGLGSTKVMEALKQFNEDILEAEMGSYGIITNSFEELEPTYVRDLKKIRNDKVWCIGPVSLSNIDELDKVQRGNSNKVLVDEWKHLKWLNSHKDESVIYASLGSLCNLTPPQLIELGYLFLY