Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99229.t1 | XP_024639431.1 | 70 | 387 | 36 | 2 | 43 | 429 | 1 | 307 | 6.60E-122 | 447.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99229.t1 | A0A396HXP9 | 70.0 | 387 | 36 | 2 | 43 | 429 | 1 | 307 | 4.7e-122 | 447.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053967 | MS.gene99229 | 0.821562 | 3.78E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99229.t1 | MTR_3g099600 | 52.653 | 245 | 98 | 3 | 43 | 285 | 1 | 229 | 2.58e-57 | 192 |
| MS.gene99229.t1 | MTR_3g099600 | 50.000 | 176 | 53 | 1 | 287 | 427 | 152 | 327 | 5.49e-35 | 133 |
| MS.gene99229.t1 | MTR_5g064800 | 83.594 | 128 | 18 | 1 | 305 | 429 | 149 | 276 | 5.55e-47 | 166 |
| MS.gene99229.t1 | MTR_5g064800 | 45.755 | 212 | 51 | 4 | 77 | 285 | 61 | 211 | 4.13e-30 | 120 |
| MS.gene99229.t1 | MTR_5g064760 | 52.083 | 96 | 39 | 3 | 321 | 415 | 125 | 214 | 1.25e-15 | 76.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99229.t1 | AT4G09060 | 44.269 | 253 | 140 | 1 | 39 | 290 | 13 | 265 | 7.10e-58 | 193 |
| MS.gene99229.t1 | AT4G09060 | 46.377 | 138 | 69 | 3 | 287 | 422 | 182 | 316 | 3.30e-20 | 91.7 |
| MS.gene99229.t1 | AT1G14680 | 45.344 | 247 | 120 | 4 | 43 | 286 | 1 | 235 | 1.34e-53 | 181 |
| MS.gene99229.t1 | AT1G14680 | 41.727 | 139 | 67 | 4 | 288 | 422 | 155 | 283 | 4.27e-18 | 84.7 |
| MS.gene99229.t1 | AT4G09060 | 40.860 | 279 | 138 | 3 | 39 | 290 | 13 | 291 | 7.79e-53 | 181 |
| MS.gene99229.t1 | AT4G09060 | 46.377 | 138 | 69 | 3 | 287 | 422 | 208 | 342 | 5.14e-20 | 91.7 |
Find 80 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTCTTCTACATATGAATTCT+TGG | 0.213648 | 5.4:-40765155 | MS.gene99229:CDS |
| TTTGATGAAATCGTCGATTT+CGG | 0.249397 | 5.4:+40767616 | None:intergenic |
| TTGATGAAATCGTCGATTTC+GGG | 0.255477 | 5.4:+40767617 | None:intergenic |
| GTCTTCTACATATGGATTCT+TGG | 0.262711 | 5.4:-40764193 | MS.gene99229:CDS |
| TGAAAATAGCATATTGAAAA+AGG | 0.281164 | 5.4:-40763297 | MS.gene99229:CDS |
| AAGTTTGCTCGACTCTCTTT+TGG | 0.304400 | 5.4:-40765200 | MS.gene99229:CDS |
| TGTTGAGGAGAATCAGAAAT+TGG | 0.314750 | 5.4:-40767302 | MS.gene99229:CDS |
| GAAGCTTGATGTCGAGGGTT+TGG | 0.314876 | 5.4:+40767555 | None:intergenic |
| AAGCTTGATGTCGAGGGTTT+GGG | 0.338482 | 5.4:+40767556 | None:intergenic |
| AAAAGGACAAAGAGCATCTA+AGG | 0.339255 | 5.4:-40763452 | MS.gene99229:CDS |
| AAAAGGACAAAGAGCATCTA+AGG | 0.339255 | 5.4:-40764299 | MS.gene99229:CDS |
| TACCTTGGCAGATGTACTAT+TGG | 0.369909 | 5.4:+40763211 | None:intergenic |
| TGGGATGTTGGAACGTGATT+TGG | 0.388755 | 5.4:-40767143 | MS.gene99229:CDS |
| GTCGAGGGTTTGGGAGGAGA+TGG | 0.389737 | 5.4:+40767565 | None:intergenic |
| GAAAGGATTCATACAGAATC+TGG | 0.394294 | 5.4:-40763245 | MS.gene99229:CDS |
| CGCTATCGAGCTACTTGTCT+TGG | 0.395328 | 5.4:+40763072 | None:intergenic |
| GGAAGGCGTGTTTGCGAGCT+GGG | 0.415287 | 5.4:-40767162 | MS.gene99229:CDS |
| TACTAAGGTTGACTCTTCTT+CGG | 0.416932 | 5.4:-40765242 | MS.gene99229:intron |
| CAATCCATCGACCACTCATT+AGG | 0.420092 | 5.4:-40767593 | MS.gene99229:CDS |
| AGCGATGAGGGAGGCGGCGA+CGG | 0.437000 | 5.4:+40769161 | None:intergenic |
| CGAGCTACTTGTCTTGGAAC+TGG | 0.447822 | 5.4:+40763078 | None:intergenic |
| AGGAAGGCGTGTTTGCGAGC+TGG | 0.456033 | 5.4:-40767163 | MS.gene99229:CDS |
| GCGAAGTGTGAGAATCTGTT+GGG | 0.460066 | 5.4:-40767277 | MS.gene99229:CDS |
| GTTGGGGCATTGTAGAGAAC+TGG | 0.463463 | 5.4:-40767260 | MS.gene99229:CDS |
| TTTCAAAATGAAGCTGAAGA+AGG | 0.465135 | 5.4:-40767190 | MS.gene99229:CDS |
| CTCACTCTCGCCGGAACCTA+AGG | 0.470941 | 5.4:-40769136 | MS.gene99229:intron |
| TCGAGGGTTTGGGAGGAGAT+GGG | 0.471014 | 5.4:+40767566 | None:intergenic |
| TCTTCTTCGGCAAGCACACA+TGG | 0.474846 | 5.4:-40765229 | MS.gene99229:CDS |
| TGAATTGATGTCTCTAGAAA+AGG | 0.480662 | 5.4:-40763469 | MS.gene99229:CDS |
| TGAATTGATGTCTCTAGAAA+AGG | 0.480662 | 5.4:-40764316 | MS.gene99229:CDS |
| AATTGTCTTACCTTAGGTTC+CGG | 0.482824 | 5.4:+40769126 | None:intergenic |
| AAAGACGAGTCTTCTACATA+TGG | 0.492701 | 5.4:-40764201 | MS.gene99229:CDS |
| AAGTAGCTCGATAGCGAAGA+AGG | 0.494411 | 5.4:-40763065 | MS.gene99229:CDS |
| AGCTGCTGTCAATGTGGAGC+GGG | 0.505097 | 5.4:-40764140 | MS.gene99229:intron |
| CGAGAGTGAGAGAGCGATGA+GGG | 0.518620 | 5.4:+40769149 | None:intergenic |
| AGCTGAAGAAGGAGCGAGGA+AGG | 0.518905 | 5.4:-40767179 | MS.gene99229:CDS |
| TGAGAGAGCGATGAGGGAGG+CGG | 0.527182 | 5.4:+40769155 | None:intergenic |
| GATGGTGATGGTGTTGTTGT+CGG | 0.530230 | 5.4:+40767652 | None:intergenic |
| GGCACGAAGCTTGATGTCGA+GGG | 0.536194 | 5.4:+40767550 | None:intergenic |
| TTGGCAGATGTACTATTGGT+AGG | 0.541731 | 5.4:+40763215 | None:intergenic |
| CGAACTCATTGAACAATCCA+AGG | 0.552460 | 5.4:-40767459 | MS.gene99229:intron |
| TAAGCTCGAAAGAGCTGAAG+AGG | 0.554165 | 5.4:-40764274 | MS.gene99229:CDS |
| TAGTGATGAAATACATGGAA+AGG | 0.556765 | 5.4:-40763262 | MS.gene99229:CDS |
| ATGTCGCCATGAGATTGAAT+CGG | 0.559587 | 5.4:-40767095 | MS.gene99229:intron |
| TGACCTCCTTGACTCTCTTG+TGG | 0.562654 | 5.4:-40764238 | MS.gene99229:CDS |
| ATGATACCGATTCAATCTCA+TGG | 0.571091 | 5.4:+40767089 | None:intergenic |
| GCTCGAAAGAGCTGAAGAGG+AGG | 0.578578 | 5.4:-40764271 | MS.gene99229:CDS |
| CCTCCTCTTCAGCTTTGTCG+AGG | 0.580231 | 5.4:+40763424 | None:intergenic |
| AGGCACGAAGCTTGATGTCG+AGG | 0.581651 | 5.4:+40767549 | None:intergenic |
| CTTGATGTCGAGGGTTTGGG+AGG | 0.585010 | 5.4:+40767559 | None:intergenic |
| TTCGGCAAGCACACATGGGG+AGG | 0.585493 | 5.4:-40765224 | MS.gene99229:CDS |
| TTCGGGTGGAAGAGACATGA+TGG | 0.585743 | 5.4:+40767634 | None:intergenic |
| TAACCTCGACAAAGCTGAAG+AGG | 0.588349 | 5.4:-40763427 | MS.gene99229:CDS |
| AGCGAAGTGTGAGAATCTGT+TGG | 0.590825 | 5.4:-40767278 | MS.gene99229:CDS |
| GGCGATAAAGAAGTTTGTTG+AGG | 0.590972 | 5.4:-40767317 | MS.gene99229:CDS |
| CATCGCTCTCTCACTCTCGC+CGG | 0.603353 | 5.4:-40769145 | MS.gene99229:CDS |
| AAAGAGCTGAAGAGGAGGTG+AGG | 0.606699 | 5.4:-40764266 | MS.gene99229:CDS |
| CTTCTTCGGCAAGCACACAT+GGG | 0.608450 | 5.4:-40765228 | MS.gene99229:CDS |
| AGGATTCATACAGAATCTGG+TGG | 0.609008 | 5.4:-40763242 | MS.gene99229:CDS |
| AAGACCTAATGAGTGGTCGA+TGG | 0.616951 | 5.4:+40767589 | None:intergenic |
| TACCAATAGTACATCTGCCA+AGG | 0.617685 | 5.4:-40763213 | MS.gene99229:intron |
| AAGGTTAGTGATGAAATACA+TGG | 0.623151 | 5.4:-40763267 | MS.gene99229:CDS |
| ATGAAATCGTCGATTTCGGG+TGG | 0.634730 | 5.4:+40767620 | None:intergenic |
| GTGTTTGCGAGCTGGGATGT+TGG | 0.635975 | 5.4:-40767155 | MS.gene99229:CDS |
| GCATTGTAGAGAACTGGAGA+AGG | 0.636476 | 5.4:-40767254 | MS.gene99229:CDS |
| TTCTTCGGCAAGCACACATG+GGG | 0.642508 | 5.4:-40765227 | MS.gene99229:CDS |
| GAGTGAGAGAGCGATGAGGG+AGG | 0.643813 | 5.4:+40769152 | None:intergenic |
| GTAGCTTAAAACAATCAACT+CGG | 0.647486 | 5.4:-40764359 | MS.gene99229:intron |
| CCTCGACAAAGCTGAAGAGG+AGG | 0.650548 | 5.4:-40763424 | MS.gene99229:intron |
| GCGAGAGTGAGAGAGCGATG+AGG | 0.652031 | 5.4:+40769148 | None:intergenic |
| AAGCTGCTGTCAATGTGGAG+CGG | 0.655453 | 5.4:-40764141 | MS.gene99229:CDS |
| GGATTCATACAGAATCTGGT+GGG | 0.658187 | 5.4:-40763241 | MS.gene99229:CDS |
| CGAAGTGTGAGAATCTGTTG+GGG | 0.661772 | 5.4:-40767276 | MS.gene99229:CDS |
| TGGAAGAGACATGATGGTGA+TGG | 0.667795 | 5.4:+40767640 | None:intergenic |
| ATGAAGCTGAAGAAGGAGCG+AGG | 0.675640 | 5.4:-40767183 | MS.gene99229:CDS |
| AGATGGGAAGACCTAATGAG+TGG | 0.676635 | 5.4:+40767582 | None:intergenic |
| GTATGAAGCGTCTGTGAATG+CGG | 0.695860 | 5.4:-40767341 | MS.gene99229:CDS |
| CTGCTGCCACAAGAGAGTCA+AGG | 0.705247 | 5.4:+40764232 | None:intergenic |
| CTGCCACAAGAGAGTCAAGG+AGG | 0.721597 | 5.4:+40764235 | None:intergenic |
| TGAAGCGTCTGTGAATGCGG+TGG | 0.757584 | 5.4:-40767338 | MS.gene99229:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 40763030 | 40771012 | 40763030 | ID=MS.gene99229 |
| chr5.4 | mRNA | 40763030 | 40771012 | 40763030 | ID=MS.gene99229.t1;Parent=MS.gene99229 |
| chr5.4 | exon | 40771007 | 40771012 | 40771007 | ID=MS.gene99229.t1.exon1;Parent=MS.gene99229.t1 |
| chr5.4 | CDS | 40771007 | 40771012 | 40771007 | ID=cds.MS.gene99229.t1;Parent=MS.gene99229.t1 |
| chr5.4 | exon | 40769137 | 40769205 | 40769137 | ID=MS.gene99229.t1.exon2;Parent=MS.gene99229.t1 |
| chr5.4 | CDS | 40769137 | 40769205 | 40769137 | ID=cds.MS.gene99229.t1;Parent=MS.gene99229.t1 |
| chr5.4 | exon | 40767460 | 40767702 | 40767460 | ID=MS.gene99229.t1.exon3;Parent=MS.gene99229.t1 |
| chr5.4 | CDS | 40767460 | 40767702 | 40767460 | ID=cds.MS.gene99229.t1;Parent=MS.gene99229.t1 |
| chr5.4 | exon | 40767096 | 40767362 | 40767096 | ID=MS.gene99229.t1.exon4;Parent=MS.gene99229.t1 |
| chr5.4 | CDS | 40767096 | 40767362 | 40767096 | ID=cds.MS.gene99229.t1;Parent=MS.gene99229.t1 |
| chr5.4 | exon | 40765103 | 40765257 | 40765103 | ID=MS.gene99229.t1.exon5;Parent=MS.gene99229.t1 |
| chr5.4 | CDS | 40765103 | 40765257 | 40765103 | ID=cds.MS.gene99229.t1;Parent=MS.gene99229.t1 |
| chr5.4 | exon | 40764141 | 40764377 | 40764141 | ID=MS.gene99229.t1.exon6;Parent=MS.gene99229.t1 |
| chr5.4 | CDS | 40764141 | 40764377 | 40764141 | ID=cds.MS.gene99229.t1;Parent=MS.gene99229.t1 |
| chr5.4 | exon | 40763425 | 40763530 | 40763425 | ID=MS.gene99229.t1.exon7;Parent=MS.gene99229.t1 |
| chr5.4 | CDS | 40763425 | 40763530 | 40763425 | ID=cds.MS.gene99229.t1;Parent=MS.gene99229.t1 |
| chr5.4 | exon | 40763214 | 40763336 | 40763214 | ID=MS.gene99229.t1.exon8;Parent=MS.gene99229.t1 |
| chr5.4 | CDS | 40763214 | 40763336 | 40763214 | ID=cds.MS.gene99229.t1;Parent=MS.gene99229.t1 |
| chr5.4 | exon | 40763030 | 40763116 | 40763030 | ID=MS.gene99229.t1.exon9;Parent=MS.gene99229.t1 |
| chr5.4 | CDS | 40763030 | 40763116 | 40763030 | ID=cds.MS.gene99229.t1;Parent=MS.gene99229.t1 |
| Gene Sequence |
| Protein sequence |
