AlfalfaGEDB | MS.gene053967

M. sativa cultivar XinJiangDaYe / MS.gene053967

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene053967.t1	XP_024639431.1	78.6	103	22	0	1	103	205	307	2.10E-26	128.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene053967.t1	A0A396HXP9	78.6	103	22	0	1	103	205	307	1.5e-26	128.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050799	MS.gene053967	0.81373	2.21E-51	-1.69E-46
MS.gene052190	MS.gene053967	0.804311	2.30E-49	-1.69E-46
MS.gene052519	MS.gene053967	0.818266	2.14E-52	-1.69E-46
MS.gene052611	MS.gene053967	0.805022	1.63E-49	-1.69E-46
MS.gene052863	MS.gene053967	0.816936	4.28E-52	-1.69E-46
MS.gene053145	MS.gene053967	0.808861	2.51E-50	-1.69E-46
MS.gene053900	MS.gene053967	0.823132	1.63E-53	-1.69E-46
MS.gene053966	MS.gene053967	0.873731	1.17E-67	-1.69E-46
MS.gene053967	MS.gene053974	0.811451	6.96E-51	-1.69E-46
MS.gene053967	MS.gene055818	0.818016	2.44E-52	-1.69E-46
MS.gene053967	MS.gene060572	0.803046	4.20E-49	-1.69E-46
MS.gene053967	MS.gene06269	0.817011	4.11E-52	-1.69E-46
MS.gene053967	MS.gene067163	0.800436	1.44E-48	-1.69E-46
MS.gene053967	MS.gene070210	0.80129	9.65E-49	-1.69E-46
MS.gene053967	MS.gene073008	0.814023	1.90E-51	-1.69E-46
MS.gene053967	MS.gene073139	0.80099	1.11E-48	-1.69E-46
MS.gene053967	MS.gene073753	0.817609	3.02E-52	-1.69E-46
MS.gene053967	MS.gene07733	0.801787	7.64E-49	-1.69E-46
MS.gene053967	MS.gene08578	0.836855	7.32E-57	-1.69E-46
MS.gene053967	MS.gene08589	0.825783	3.88E-54	-1.69E-46
MS.gene053967	MS.gene08853	0.807653	4.55E-50	-1.69E-46
MS.gene053967	MS.gene08854	0.808435	3.10E-50	-1.69E-46
MS.gene053967	MS.gene23190	0.808682	2.75E-50	-1.69E-46
MS.gene053967	MS.gene25778	0.8108	9.63E-51	-1.69E-46
MS.gene053967	MS.gene31283	0.816688	4.86E-52	-1.69E-46
MS.gene053967	MS.gene33624	0.805601	1.23E-49	-1.69E-46
MS.gene053967	MS.gene35408	0.803191	3.92E-49	-1.69E-46
MS.gene053967	MS.gene44316	0.80971	1.65E-50	-1.69E-46
MS.gene053967	MS.gene45641	0.806206	9.21E-50	-1.69E-46
MS.gene053967	MS.gene47832	0.803705	3.07E-49	-1.69E-46
MS.gene053967	MS.gene49283	0.83108	2.04E-55	-1.69E-46
MS.gene053967	MS.gene51459	0.841547	4.45E-58	-1.69E-46
MS.gene053967	MS.gene51511	0.807862	4.11E-50	-1.69E-46
MS.gene053967	MS.gene51818	0.802224	6.21E-49	-1.69E-46
MS.gene053967	MS.gene53586	0.816917	4.32E-52	-1.69E-46
MS.gene053967	MS.gene55111	0.825694	4.08E-54	-1.69E-46
MS.gene053967	MS.gene57027	0.809826	1.56E-50	-1.69E-46
MS.gene053967	MS.gene58686	0.8437	1.19E-58	-1.69E-46
MS.gene053967	MS.gene59269	0.805371	1.38E-49	-1.69E-46
MS.gene053967	MS.gene64249	0.835552	1.57E-56	-1.69E-46
MS.gene053967	MS.gene65271	0.818423	1.98E-52	-1.69E-46
MS.gene053967	MS.gene67746	0.806493	8.01E-50	-1.69E-46
MS.gene053967	MS.gene68390	0.827421	1.58E-54	-1.69E-46
MS.gene053967	MS.gene68826	0.806919	6.51E-50	-1.69E-46
MS.gene053967	MS.gene71781	0.807742	4.36E-50	-1.69E-46
MS.gene053967	MS.gene72268	0.827025	1.96E-54	-1.69E-46
MS.gene053967	MS.gene72347	0.833023	6.77E-56	-1.69E-46
MS.gene053967	MS.gene73564	0.814509	1.49E-51	-1.69E-46
MS.gene053967	MS.gene75003	0.808786	2.61E-50	-1.69E-46
MS.gene053967	MS.gene75134	0.809572	1.77E-50	-1.69E-46
MS.gene053967	MS.gene78775	0.902798	6.70E-79	-1.69E-46
MS.gene053967	MS.gene79285	0.804889	1.74E-49	-1.69E-46
MS.gene053967	MS.gene81329	0.806432	8.25E-50	-1.69E-46
MS.gene053967	MS.gene82397	-0.818509	1.89E-52	-1.69E-46
MS.gene053967	MS.gene85604	0.806961	6.38E-50	-1.69E-46
MS.gene053967	MS.gene85606	0.806387	8.43E-50	-1.69E-46
MS.gene053967	MS.gene86086	0.81748	3.23E-52	-1.69E-46
MS.gene053967	MS.gene87354	0.817903	2.59E-52	-1.69E-46
MS.gene053967	MS.gene91337	0.823028	1.73E-53	-1.69E-46
MS.gene053967	MS.gene92383	0.803745	3.01E-49	-1.69E-46
MS.gene053967	MS.gene93311	0.814061	1.87E-51	-1.69E-46
MS.gene053967	MS.gene93764	0.809653	1.70E-50	-1.69E-46
MS.gene053967	MS.gene96242	0.805258	1.46E-49	-1.69E-46
MS.gene053967	MS.gene98774	0.813531	2.44E-51	-1.69E-46
MS.gene053967	MS.gene99229	0.821562	3.78E-53	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 17 sgRNAs with CRISPR-Local

Find 35 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGAAAATAGCATATTGAAAA+AGG	0.281164	5.2:-42964505	MS.gene053967:CDS
AAAAGGACAAAGAGCATCTA+AGG	0.339255	5.2:-42964660	MS.gene053967:CDS
TACCTTGGCAGATGTACTAT+TGG	0.369909	5.2:+42964419	None:intergenic
GAAAGGATTCATACAGAATC+TGG	0.394294	5.2:-42964453	MS.gene053967:CDS
CGCTATCGAGCTACTTGTCT+TGG	0.395328	5.2:+42964280	None:intergenic
CGAGCTACTTGTCTTGGAAC+TGG	0.447822	5.2:+42964286	None:intergenic
TGAATTGATGTCTCTAGAAA+AGG	0.480662	5.2:-42964677	MS.gene053967:CDS
AAGTAGCTCGATAGCGAAGA+AGG	0.494411	5.2:-42964273	MS.gene053967:CDS
TTGGCAGATGTACTATTGGT+AGG	0.541731	5.2:+42964423	None:intergenic
TAGTGATGAAATACATGGAA+AGG	0.556765	5.2:-42964470	MS.gene053967:CDS
CCTCCTCTTCAGCTTTGTCG+AGG	0.580231	5.2:+42964632	None:intergenic
TAACCTCGACAAAGCTGAAG+AGG	0.588349	5.2:-42964635	MS.gene053967:CDS
AGGATTCATACAGAATCTGG+TGG	0.609008	5.2:-42964450	MS.gene053967:CDS
TACCAATAGTACATCTGCCA+AGG	0.617685	5.2:-42964421	MS.gene053967:intron
AAGGTTAGTGATGAAATACA+TGG	0.623151	5.2:-42964475	MS.gene053967:CDS
CCTCGACAAAGCTGAAGAGG+AGG	0.650548	5.2:-42964632	MS.gene053967:intron
GGATTCATACAGAATCTGGT+GGG	0.658187	5.2:-42964449	MS.gene053967:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AATGCAATTAATTACTTAAA+AGG	+	chr5.2:42964630-42964649	None:intergenic	15.0%
!!!	TTTACAGTTTTTGAATTATA+TGG	-	chr5.2:42964382-42964401	MS.gene053967:intron	15.0%
!!	TGAAAATAGCATATTGAAAA+AGG	-	chr5.2:42964453-42964472	MS.gene053967:CDS	20.0%
!	GCAATTAATTACTTAAAAGG+AGG	+	chr5.2:42964627-42964646	None:intergenic	25.0%
	AAGGTTAGTGATGAAATACA+TGG	-	chr5.2:42964483-42964502	MS.gene053967:CDS	30.0%
	ATTATATGGAATGACTGACT+TGG	-	chr5.2:42964396-42964415	MS.gene053967:intron	30.0%
	GTAAAAAGTCGACAGTAAAA+GGG	+	chr5.2:42964367-42964386	None:intergenic	30.0%
	TAAAAAGTCGACAGTAAAAG+GGG	+	chr5.2:42964366-42964385	None:intergenic	30.0%
	TAGTGATGAAATACATGGAA+AGG	-	chr5.2:42964488-42964507	MS.gene053967:CDS	30.0%
	TATTGAAAAAGGAGAACAGA+AGG	-	chr5.2:42964464-42964483	MS.gene053967:CDS	30.0%
	TGTAAAAAGTCGACAGTAAA+AGG	+	chr5.2:42964368-42964387	None:intergenic	30.0%
!	TGAATTGATGTCTCTAGAAA+AGG	-	chr5.2:42964281-42964300	MS.gene053967:CDS	30.0%
	AAAAAGTCGACAGTAAAAGG+GGG	+	chr5.2:42964365-42964384	None:intergenic	35.0%
	GAAAGGATTCATACAGAATC+TGG	-	chr5.2:42964505-42964524	MS.gene053967:CDS	35.0%
!!	AAAAGGACAAAGAGCATCTA+AGG	-	chr5.2:42964298-42964317	MS.gene053967:CDS	35.0%
	AGGATTCATACAGAATCTGG+TGG	-	chr5.2:42964508-42964527	MS.gene053967:CDS	40.0%
	CTGCCAAGGTAAGAATCATA+GGG	-	chr5.2:42964551-42964570	MS.gene053967:intron	40.0%
	GGATTCATACAGAATCTGGT+GGG	-	chr5.2:42964509-42964528	MS.gene053967:CDS	40.0%
	TACCAATAGTACATCTGCCA+AGG	-	chr5.2:42964537-42964556	MS.gene053967:intron	40.0%
	TACCTTGGCAGATGTACTAT+TGG	+	chr5.2:42964542-42964561	None:intergenic	40.0%
	TCTGCCAAGGTAAGAATCAT+AGG	-	chr5.2:42964550-42964569	MS.gene053967:intron	40.0%
	TGCCAAGGTAAGAATCATAG+GGG	-	chr5.2:42964552-42964571	MS.gene053967:intron	40.0%
	TTGGCAGATGTACTATTGGT+AGG	+	chr5.2:42964538-42964557	None:intergenic	40.0%
!	ACAGAGAGTAGTTTTCCCTA+TGG	+	chr5.2:42964433-42964452	None:intergenic	40.0%
!!	TTGGCTTGGTGTAAACCATA+GGG	-	chr5.2:42964415-42964434	MS.gene053967:intron	40.0%
	AAGTAGCTCGATAGCGAAGA+AGG	-	chr5.2:42964685-42964704	MS.gene053967:CDS	45.0%
	CACCCCTATGATTCTTACCT+TGG	+	chr5.2:42964557-42964576	None:intergenic	45.0%
	TAACCTCGACAAAGCTGAAG+AGG	-	chr5.2:42964323-42964342	MS.gene053967:intron	45.0%
!!	ATGGAATGACTGACTTGGCT+TGG	-	chr5.2:42964401-42964420	MS.gene053967:intron	45.0%
!!	CTTGGCTTGGTGTAAACCAT+AGG	-	chr5.2:42964414-42964433	MS.gene053967:intron	45.0%
	ACAAAGCTGAAGAGGAGGTG+AGG	-	chr5.2:42964331-42964350	MS.gene053967:intron	50.0%
	CGAGCTACTTGTCTTGGAAC+TGG	+	chr5.2:42964675-42964694	None:intergenic	50.0%
	CGCTATCGAGCTACTTGTCT+TGG	+	chr5.2:42964681-42964700	None:intergenic	50.0%
	CCTCGACAAAGCTGAAGAGG+AGG	-	chr5.2:42964326-42964345	MS.gene053967:intron	55.0%
!	CCTCCTCTTCAGCTTTGTCG+AGG	+	chr5.2:42964329-42964348	None:intergenic	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.2	gene	42964242	42964738	42964242	ID=MS.gene053967
chr5.2	mRNA	42964242	42964738	42964242	ID=MS.gene053967.t1;Parent=MS.gene053967
chr5.2	exon	42964633	42964738	42964633	ID=MS.gene053967.t1.exon1;Parent=MS.gene053967.t1
chr5.2	CDS	42964633	42964738	42964633	ID=cds.MS.gene053967.t1;Parent=MS.gene053967.t1
chr5.2	exon	42964422	42964544	42964422	ID=MS.gene053967.t1.exon2;Parent=MS.gene053967.t1
chr5.2	CDS	42964422	42964544	42964422	ID=cds.MS.gene053967.t1;Parent=MS.gene053967.t1
chr5.2	exon	42964242	42964324	42964242	ID=MS.gene053967.t1.exon3;Parent=MS.gene053967.t1
chr5.2	CDS	42964242	42964324	42964242	ID=cds.MS.gene053967.t1;Parent=MS.gene053967.t1

Gene Sequence

>MS.gene053967

CTTAAAACAATCAACTCAGAGATTATTGTCTCTTGTTGCTGAATTGATGTCTCTAGAAAAGGACAAAGAGCATCTAAGGATTAACCTCGACAAAGCTGAAGAGGAGGTGAGGATTGCTGACCCCCTTTTACTGTCGACTTTTTACAGTTTTTGAATTATATGGAATGACTGACTTGGCTTGGTGTAAACCATAGGGAAAACTACTCTCTGTTGAAAATAGCATATTGAAAAAGGAGAACAGAAGGTTAGTGATGAAATACATGGAAAGGATTCATACAGAATCTGGTGGGAAGCCTACCAATAGTACATCTGCCAAGGTAAGAATCATAGGGGTGTGTTGATTTTGCAACGACTGTTCAGTTTCGTTTCATTTCATTTCGCTCCTCCTTTTAAGTAATTAATTGCATTTTGCAGTCAAACAAGAGAAAGTCCAGTTCCAAGACAAGTAGCTCGATAGCGAAGAAGGTTGATTTTGATGATTTAGATTCACTTGCAAA

Protein sequence

>MS.gene053967.t1

LKTINSEIIVSCCUIDVSRKGQRASKDUPRQSURGGKTTLCUKUHIEKGEQKVSDEIHGKDSYRIWWEAYQUYICQVKQEKVQFQDKULDSEEGUFUUFRFTCK