AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene99275

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99275.t1 MTR_5g044770 96.056 431 17 0 1 431 1 431 0.0 850
MS.gene99275.t1 MTR_8g096990 73.148 432 115 1 1 431 1 432 0.0 651
MS.gene99275.t1 MTR_5g080960 72.644 435 114 4 1 431 1 434 0.0 640
MS.gene99275.t1 MTR_5g080890 71.954 435 117 4 1 431 1 434 0.0 636
MS.gene99275.t1 MTR_8g038280 69.495 436 128 2 1 431 1 436 0.0 623
MS.gene99275.t1 MTR_8g038250 68.950 438 107 3 1 431 1 416 0.0 603
MS.gene99275.t1 MTR_6g090290 64.450 436 149 3 1 430 1 436 0.0 551
MS.gene99275.t1 MTR_8g038310 62.963 432 139 5 1 430 1 413 0.0 545
MS.gene99275.t1 MTR_4g109760 63.741 433 121 6 1 431 1 399 3.07e-177 501
MS.gene99275.t1 MTR_3g028120 52.029 419 192 5 22 431 21 439 4.33e-145 421
MS.gene99275.t1 MTR_2g067830 54.433 406 178 6 32 430 1 406 4.08e-144 417
MS.gene99275.t1 MTR_6g088760 52.195 410 186 6 1 401 1 409 7.55e-138 405
MS.gene99275.t1 MTR_5g038360 48.843 432 208 7 9 430 8 436 1.04e-137 402
MS.gene99275.t1 MTR_0024s0040 50.605 413 189 7 30 430 1 410 1.09e-132 388
MS.gene99275.t1 MTR_2g105300 51.613 403 166 8 32 427 1 381 2.01e-129 379
MS.gene99275.t1 MTR_5g044570 45.995 437 192 11 6 430 7 411 1.58e-123 365
MS.gene99275.t1 MTR_0036s0050 44.165 437 222 12 9 430 6 435 1.44e-110 332
MS.gene99275.t1 MTR_0036s0070 41.898 432 230 11 9 430 6 426 3.42e-108 326
MS.gene99275.t1 MTR_5g008070 40.338 414 229 7 21 428 23 424 1.10e-100 307
MS.gene99275.t1 MTR_2g059490 46.011 376 190 8 62 428 37 408 1.39e-100 307
MS.gene99275.t1 MTR_0036s0110 40.265 452 238 12 1 430 1 442 1.07e-99 305
MS.gene99275.t1 MTR_5g008150 41.053 380 213 7 59 430 36 412 6.27e-94 289
MS.gene99275.t1 MTR_2g059460 40.482 415 201 11 26 430 28 406 4.53e-91 281
MS.gene99275.t1 MTR_5g008110 40.650 369 207 7 70 430 51 415 3.05e-89 277
MS.gene99275.t1 MTR_3g097150 37.079 445 250 14 6 431 16 449 2.01e-82 260
MS.gene99275.t1 MTR_1g060750 36.476 403 216 12 40 430 2 376 7.33e-73 233
MS.gene99275.t1 MTR_4g045857 46.502 243 124 4 74 310 46 288 1.07e-65 212
MS.gene99275.t1 MTR_1g115210 37.960 353 199 11 85 428 145 486 1.28e-61 207
MS.gene99275.t1 MTR_5g008090 38.721 297 171 7 142 430 21 314 2.92e-60 199
MS.gene99275.t1 MTR_7g117430 33.724 427 244 17 25 428 74 484 1.47e-58 199
MS.gene99275.t1 MTR_1g039270 35.310 371 210 12 70 428 117 469 5.37e-58 197
MS.gene99275.t1 MTR_6g085010 34.417 369 223 10 66 428 136 491 2.59e-52 183
MS.gene99275.t1 MTR_2g028190 31.648 455 267 15 9 428 6 451 2.10e-50 177
MS.gene99275.t1 MTR_1g111350 31.818 418 243 14 21 428 82 467 2.94e-50 177
MS.gene99275.t1 MTR_6g088765 41.016 256 110 5 160 414 3 218 7.42e-50 169
MS.gene99275.t1 MTR_3g046300 33.073 384 236 9 68 431 79 461 2.48e-49 174
MS.gene99275.t1 MTR_5g091130 31.061 396 251 10 57 431 162 556 3.79e-46 167
MS.gene99275.t1 MTR_4g007740 31.931 404 231 15 49 428 94 477 4.20e-46 166
MS.gene99275.t1 MTR_3g053710 33.333 363 217 12 82 428 113 466 1.34e-43 159
MS.gene99275.t1 MTR_8g103880 30.233 344 219 12 87 421 117 448 9.88e-37 140
MS.gene99275.t1 MTR_2g055840 28.084 381 232 13 78 428 5 373 2.36e-34 132
MS.gene99275.t1 MTR_2g070430 27.689 437 270 14 27 426 24 451 3.12e-34 133
MS.gene99275.t1 MTR_3g068000 29.581 382 211 14 89 428 76 441 4.53e-32 127
MS.gene99275.t1 MTR_3g053980 31.183 372 226 13 73 428 128 485 1.11e-30 123
MS.gene99275.t1 MTR_7g080230 27.356 435 261 17 32 428 13 430 4.20e-30 122
MS.gene99275.t1 MTR_4g008110 53.448 116 31 1 158 273 45 137 3.48e-29 111
MS.gene99275.t1 MTR_7g105850 28.211 475 274 20 9 430 7 467 2.11e-28 117
MS.gene99275.t1 MTR_3g065160 26.344 372 228 12 88 431 76 429 8.72e-28 114
MS.gene99275.t1 MTR_8g075090 27.470 415 256 17 40 430 68 461 2.34e-27 114
MS.gene99275.t1 MTR_8g075090 27.603 413 254 17 40 428 68 459 2.77e-27 113
MS.gene99275.t1 MTR_5g022880 27.211 441 265 17 27 428 38 461 1.35e-26 112
MS.gene99275.t1 MTR_8g094590 27.394 376 224 17 85 431 72 427 1.73e-26 110
MS.gene99275.t1 MTR_4g095270 26.196 397 240 18 57 431 61 426 6.33e-26 110
MS.gene99275.t1 MTR_4g095270 26.196 397 240 18 57 431 61 426 6.66e-26 110
MS.gene99275.t1 MTR_7g062360 26.963 382 225 14 89 431 62 428 6.88e-26 109
MS.gene99275.t1 MTR_8g075090 28.351 388 233 17 40 403 68 434 1.32e-25 108
MS.gene99275.t1 MTR_6g084710 27.338 417 249 20 49 428 151 550 2.90e-25 108
MS.gene99275.t1 MTR_5g016260 24.937 397 239 18 57 428 69 431 6.13e-25 107
MS.gene99275.t1 MTR_1g078240 27.273 374 230 15 85 428 68 429 6.29e-25 107
MS.gene99275.t1 MTR_5g016260 24.937 397 239 18 57 428 69 431 6.46e-25 107
MS.gene99275.t1 MTR_6g084710 29.179 329 202 15 49 363 151 462 9.65e-25 106
MS.gene99275.t1 MTR_1g061940 29.380 371 227 15 85 428 82 444 1.07e-24 106
MS.gene99275.t1 MTR_1g078240 27.467 375 229 16 85 428 68 430 1.54e-24 105
MS.gene99275.t1 MTR_3g096930 25.532 376 230 16 85 431 83 437 1.68e-24 105
MS.gene99275.t1 MTR_2g084550 30.645 372 220 15 85 428 76 437 4.48e-24 104
MS.gene99275.t1 MTR_2g103340 40.580 138 24 2 21 144 34 127 1.03e-23 98.2
MS.gene99275.t1 MTR_8g094590 28.933 356 205 17 85 416 72 403 1.53e-23 102
MS.gene99275.t1 MTR_7g021510 27.056 377 228 17 85 428 71 433 8.21e-23 100
MS.gene99275.t1 MTR_8g094590 30.888 259 149 10 85 333 72 310 9.33e-23 98.6
MS.gene99275.t1 MTR_3g087780 26.269 453 261 22 24 428 23 450 1.35e-22 100
MS.gene99275.t1 MTR_1g029810 25.995 377 229 17 85 431 56 412 1.50e-22 99.8
MS.gene99275.t1 MTR_1g029810 25.995 377 229 17 85 431 56 412 1.53e-22 99.4
MS.gene99275.t1 MTR_7g102750 27.423 423 251 20 45 428 35 440 2.64e-22 99.4
MS.gene99275.t1 MTR_2g063110 24.375 480 270 18 27 428 44 508 2.79e-22 99.4
MS.gene99275.t1 MTR_4g100990 25.791 411 233 18 84 431 72 473 8.63e-22 97.8
MS.gene99275.t1 MTR_4g023650 26.429 420 251 20 50 431 23 422 1.66e-21 96.7
MS.gene99275.t1 MTR_4g056420 28.302 371 229 16 85 428 76 436 2.24e-21 96.7
MS.gene99275.t1 MTR_5g012490 25.572 481 271 22 14 428 10 469 7.50e-21 95.1
MS.gene99275.t1 MTR_1g078240 28.155 309 188 13 85 371 68 364 9.28e-21 94.0
MS.gene99275.t1 MTR_8g075010 25.135 370 234 14 87 431 101 452 1.78e-20 94.0
MS.gene99275.t1 MTR_1g037630 26.714 423 269 17 26 428 27 428 3.22e-20 92.8
MS.gene99275.t1 MTR_5g061690 28.940 349 200 19 93 416 108 433 4.04e-20 93.2
MS.gene99275.t1 MTR_3g014290 27.479 353 228 12 87 428 81 416 5.95e-20 91.7
MS.gene99275.t1 MTR_0945s0010 38.211 123 72 3 309 428 1 122 1.59e-19 85.5
MS.gene99275.t1 MTR_5g077850 25.397 378 228 11 88 431 83 440 2.04e-19 90.5
MS.gene99275.t1 MTR_7g102750 27.481 393 230 19 45 399 35 410 5.08e-19 89.4
MS.gene99275.t1 MTR_7g080230 26.437 348 215 14 110 428 25 360 1.28e-18 87.8
MS.gene99275.t1 MTR_8g039540 25.814 430 284 16 5 416 9 421 2.62e-18 87.0
MS.gene99275.t1 MTR_4g023630 24.641 418 261 19 50 431 23 422 6.31e-18 85.9
MS.gene99275.t1 MTR_3g087780 28.840 319 189 16 129 428 9 308 8.27e-18 85.1
MS.gene99275.t1 MTR_3g087780 28.840 319 189 16 129 428 45 344 9.49e-18 85.1
MS.gene99275.t1 MTR_5g061690 28.571 294 173 17 138 416 23 294 5.71e-15 76.6
MS.gene99275.t1 MTR_5g077920 24.219 384 249 12 76 430 58 428 5.48e-14 73.9
MS.gene99275.t1 MTR_3g100500 25.879 398 256 14 52 428 58 437 1.33e-13 72.8
MS.gene99275.t1 MTR_2g037710 28.846 208 119 7 230 428 18 205 7.99e-13 67.8
MS.gene99275.t1 MTR_3g006820 22.680 388 230 16 85 431 57 415 7.71e-12 67.0
MS.gene99275.t1 MTR_2g037650 32.456 114 59 3 64 174 99 197 5.66e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99275.t1 AT1G64830 50.123 407 199 4 25 428 23 428 1.71e-137 401
MS.gene99275.t1 AT5G33340 49.755 408 200 5 28 431 31 437 1.21e-130 384
MS.gene99275.t1 AT2G35615 45.434 438 215 9 14 428 7 443 6.25e-114 342
MS.gene99275.t1 AT1G31450 42.472 445 235 8 1 428 1 441 4.64e-106 321
MS.gene99275.t1 AT2G28010 40.920 435 204 12 1 428 3 391 4.00e-91 281
MS.gene99275.t1 AT2G28040 41.050 419 195 12 16 428 18 390 3.41e-84 263
MS.gene99275.t1 AT2G28040 43.767 377 183 11 58 428 28 381 4.57e-84 263
MS.gene99275.t1 AT2G28220 38.391 435 211 14 7 428 7 397 7.57e-81 255
MS.gene99275.t1 AT2G28030 44.126 349 166 10 87 428 61 387 2.90e-80 253
MS.gene99275.t1 AT2G28225 43.343 353 167 12 87 428 67 397 3.89e-79 251
MS.gene99275.t1 AT2G03200 36.806 432 236 14 25 431 42 461 1.15e-72 236
MS.gene99275.t1 AT3G20015 32.192 438 250 16 20 428 51 470 2.69e-57 196
MS.gene99275.t1 AT3G18490 31.915 423 263 14 18 428 91 500 4.89e-57 196
MS.gene99275.t1 AT1G01300 36.158 354 207 11 85 428 140 484 2.47e-56 193
MS.gene99275.t1 AT3G61820 36.188 362 202 14 85 428 133 483 1.92e-52 183
MS.gene99275.t1 AT4G30030 34.316 373 217 13 70 428 61 419 8.21e-52 180
MS.gene99275.t1 AT1G25510 33.989 356 205 12 85 428 146 483 8.79e-50 176
MS.gene99275.t1 AT5G10760 34.243 403 235 14 31 428 87 464 1.91e-47 169
MS.gene99275.t1 AT2G23945 26.957 460 281 14 9 431 10 451 4.82e-47 168
MS.gene99275.t1 AT5G10770 32.000 425 252 15 29 428 61 473 1.75e-46 167
MS.gene99275.t1 AT3G12700 30.626 431 275 14 16 428 36 460 3.89e-46 166
MS.gene99275.t1 AT3G25700 30.027 373 227 13 85 428 82 449 6.75e-46 165
MS.gene99275.t1 AT4G30040 31.828 443 257 17 6 423 5 427 7.67e-45 161
MS.gene99275.t1 AT2G42980 32.376 383 230 14 75 431 148 527 7.62e-43 158
MS.gene99275.t1 AT3G59080 31.250 384 237 12 72 431 155 535 3.95e-42 156
MS.gene99275.t1 AT1G79720 31.405 363 213 14 87 428 135 482 8.49e-39 146
MS.gene99275.t1 AT5G36260 27.880 434 251 18 28 428 29 433 7.91e-35 135
MS.gene99275.t1 AT3G59080 28.760 379 217 11 72 431 155 499 3.82e-33 130
MS.gene99275.t1 AT3G02740 29.243 383 219 18 76 428 79 439 3.78e-32 127
MS.gene99275.t1 AT3G02740 28.571 371 220 16 87 428 10 364 1.43e-31 125
MS.gene99275.t1 AT2G39710 28.267 375 213 14 89 423 67 425 4.60e-30 121
MS.gene99275.t1 AT1G65240 28.649 370 223 17 85 428 72 426 1.48e-29 120
MS.gene99275.t1 AT3G54400 28.125 352 216 13 62 396 56 387 3.61e-28 115
MS.gene99275.t1 AT5G43100 26.620 432 253 19 28 428 19 417 4.61e-27 113
MS.gene99275.t1 AT5G43100 26.620 432 253 19 28 428 19 417 1.33e-26 112
MS.gene99275.t1 AT5G02190 27.056 377 215 15 96 428 82 442 3.86e-26 110
MS.gene99275.t1 AT2G17760 27.553 421 256 18 32 428 54 449 4.00e-25 107
MS.gene99275.t1 AT5G37540 26.005 373 229 13 88 428 81 438 5.39e-25 107
MS.gene99275.t1 AT3G50050 26.503 449 273 20 12 428 13 436 6.07e-25 107
MS.gene99275.t1 AT1G49050 25.070 355 232 13 104 430 49 397 1.72e-24 105
MS.gene99275.t1 AT1G44130 25.500 400 243 17 67 431 23 402 2.84e-24 104
MS.gene99275.t1 AT1G49050 24.802 379 243 15 85 430 201 570 3.68e-24 105
MS.gene99275.t1 AT5G45120 27.228 404 226 18 87 429 83 479 5.45e-24 104
MS.gene99275.t1 AT4G33490 26.044 407 235 19 63 428 30 411 7.54e-24 103
MS.gene99275.t1 AT1G05840 26.933 375 229 16 85 428 78 438 1.76e-23 102
MS.gene99275.t1 AT1G09750 27.042 355 240 11 85 428 102 448 2.04e-23 102
MS.gene99275.t1 AT2G36670 28.000 375 224 19 85 428 98 457 8.68e-23 100
MS.gene99275.t1 AT1G66180 27.005 374 226 13 88 431 73 429 8.79e-23 100
MS.gene99275.t1 AT2G36670 27.937 383 226 20 81 428 95 462 2.29e-22 99.8
MS.gene99275.t1 AT2G36670 27.749 382 230 19 78 428 25 391 2.66e-22 99.0
MS.gene99275.t1 AT2G36670 27.749 382 230 19 78 428 25 391 2.66e-22 99.0
MS.gene99275.t1 AT5G22850 30.053 376 217 17 85 428 79 440 4.11e-22 99.0
MS.gene99275.t1 AT3G51350 25.951 447 266 21 28 430 29 454 1.33e-21 97.4
MS.gene99275.t1 AT1G08210 27.778 378 220 19 85 428 130 488 4.93e-21 95.9
MS.gene99275.t1 AT1G08210 27.778 378 220 19 85 428 82 440 4.93e-21 95.5
MS.gene99275.t1 AT4G33490 28.344 314 176 16 63 352 27 315 5.21e-21 94.7
MS.gene99275.t1 AT1G08210 27.778 378 220 19 85 428 116 474 5.92e-21 95.5
MS.gene99275.t1 AT3G51360 27.152 453 268 22 14 428 17 445 7.81e-21 95.1
MS.gene99275.t1 AT3G51360 27.152 453 268 22 14 428 7 435 8.66e-21 94.7
MS.gene99275.t1 AT1G49050 27.519 258 172 9 85 333 201 452 9.14e-21 94.7
MS.gene99275.t1 AT3G52500 27.964 447 244 20 47 428 34 467 1.91e-20 93.6
MS.gene99275.t1 AT4G12920 26.124 356 231 14 83 428 54 387 2.43e-20 92.8
MS.gene99275.t1 AT3G51360 28.846 364 210 18 92 428 10 351 2.57e-20 92.8
MS.gene99275.t1 AT4G35880 23.555 467 293 19 8 431 9 454 4.75e-19 89.7
MS.gene99275.t1 AT5G10080 25.210 476 273 27 8 428 6 453 3.06e-18 87.4
MS.gene99275.t1 AT3G51360 27.470 415 241 21 14 392 7 397 1.09e-17 85.1
MS.gene99275.t1 AT3G51360 27.470 415 241 21 14 392 17 407 1.34e-17 85.1
MS.gene99275.t1 AT1G08210 26.173 405 219 20 85 428 82 467 2.33e-17 84.7
MS.gene99275.t1 AT3G51360 26.804 388 232 17 14 371 7 372 5.47e-17 82.8
MS.gene99275.t1 AT3G51360 26.804 388 232 17 14 371 17 382 6.91e-17 82.8
MS.gene99275.t1 AT4G35880 23.864 440 271 19 8 404 9 427 3.12e-16 80.9
MS.gene99275.t1 AT5G07030 28.349 321 201 13 87 396 115 417 9.72e-16 79.3
MS.gene99275.t1 AT3G25700 27.660 141 94 3 85 217 82 222 1.10e-15 78.6
MS.gene99275.t1 AT3G51360 29.154 319 186 17 128 428 13 309 3.58e-15 77.0
MS.gene99275.t1 AT3G51360 29.154 319 186 17 128 428 13 309 3.58e-15 77.0
MS.gene99275.t1 AT3G51360 26.531 343 209 14 14 332 17 340 1.17e-14 75.5
MS.gene99275.t1 AT4G16563 25.653 421 230 17 83 428 79 491 2.74e-13 72.0
MS.gene99275.t1 AT3G42550 30.769 104 70 1 87 188 78 181 4.64e-11 64.7

Find 82 sgRNAs with CRISPR-Local

Find 95 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTCTCATGTTTATACTAATT+TGG 0.143876 5.4:-34286171 MS.gene99275:CDS
GGAGATGCCGCTATGGTTTC+TGG 0.152074 5.4:-34286362 MS.gene99275:CDS
TCTCAAACTGGTTCCATCTT+TGG 0.202744 5.4:-34285948 MS.gene99275:CDS
AAGACGAGCAAACTCAATTT+TGG 0.203851 5.4:-34286383 MS.gene99275:CDS
TTAAAATGTGCAGTGATTAT+AGG 0.219892 5.4:+34286050 None:intergenic
ACATGAGCAAAGGTACTAAT+TGG 0.237962 5.4:+34286005 None:intergenic
ATACAGGTAGTGACATTATT+TGG 0.244218 5.4:-34286820 MS.gene99275:CDS
CAAAGGTTCAATAGTTGATT+AGG 0.274777 5.4:+34286104 None:intergenic
ACAACACCATATACATTAAA+AGG 0.278090 5.4:+34286845 None:intergenic
GGAAACAAAAGAATAGAATT+TGG 0.282532 5.4:-34286260 MS.gene99275:CDS
TCATTTCCTAAAACTGTTAT+CGG 0.289191 5.4:-34286557 MS.gene99275:CDS
TTACTGTTTGTTGCCGTTGA+TGG 0.300361 5.4:-34286417 MS.gene99275:CDS
GGTATAGTTGGTCTTGGAAT+TGG 0.333564 5.4:-34286491 MS.gene99275:CDS
AAAGAAAAGAAGGGTAATAA+AGG 0.341917 5.4:+34287120 None:intergenic
TGAGTATTTGATGACTTATT+CGG 0.350299 5.4:-34286879 MS.gene99275:CDS
TAAATGATTGGCACGATTGA+TGG 0.356896 5.4:+34286955 None:intergenic
CTTGGCACAACTAAATTTGT+TGG 0.360015 5.4:-34285922 MS.gene99275:CDS
TTGACATATACAGTGGATTC+AGG 0.375476 5.4:+34286908 None:intergenic
TTGTGCCAAGTTTCCAAAGA+TGG 0.388546 5.4:+34285935 None:intergenic
ATGTTTCAAGGGGCAACTTC+CGG 0.393396 5.4:-34286512 MS.gene99275:CDS
GGTAACATCATACTTGATTC+AGG 0.399481 5.4:-34286215 MS.gene99275:CDS
TGGTTCCATCTTTGGAAACT+TGG 0.401441 5.4:-34285940 MS.gene99275:CDS
TATGTCTCTTACAACACAAT+TGG 0.409960 5.4:-34286465 MS.gene99275:CDS
ACACAATTGGAATCTTCAAT+TGG 0.412966 5.4:-34286452 MS.gene99275:CDS
TGTTTATACTAATTTGGAAT+CGG 0.430186 5.4:-34286165 MS.gene99275:CDS
ACTTCCGGTATAGTTGGTCT+TGG 0.461194 5.4:-34286497 MS.gene99275:CDS
CCTAAAACTGTTATCGGATG+TGG 0.461217 5.4:-34286551 MS.gene99275:CDS
AATAATACAGGGATGTTTCA+AGG 0.462978 5.4:-34286524 MS.gene99275:CDS
TGCTTGTAGCATTGTTCACA+AGG 0.465026 5.4:+34286785 None:intergenic
TACTAATTTGGAATCGGCGG+TGG 0.466564 5.4:-34286159 MS.gene99275:CDS
GGATGTGGACACAATAATAC+AGG 0.478375 5.4:-34286536 MS.gene99275:CDS
ACCTTTGCTCATGTTGCTGA+TGG 0.482020 5.4:-34285996 MS.gene99275:CDS
AATTGTGTTGTAAGAGACAT+AGG 0.482732 5.4:+34286467 None:intergenic
ACATTAGAAGCATTTAGTGT+TGG 0.483347 5.4:-34286281 MS.gene99275:CDS
GGAATCGGCGGTGGCACGAT+TGG 0.484814 5.4:-34286150 MS.gene99275:CDS
AACAAAGGGAAAAGAAAAGA+AGG 0.487257 5.4:+34287110 None:intergenic
TGCTCATGTTGCTGATGGTG+TGG 0.491769 5.4:-34285991 MS.gene99275:CDS
TGAAACAGAATGACCGGTGG+TGG 0.491841 5.4:+34286577 None:intergenic
GGAATCTTGAAATAAATGAT+TGG 0.494179 5.4:+34286943 None:intergenic
TAGTGTTGAGACTCTTACAT+TGG 0.503954 5.4:-34286603 MS.gene99275:CDS
GCACAACTAAATTTGTTGGT+TGG 0.505438 5.4:-34285918 MS.gene99275:CDS
AAAAGAAATAATGAAACAAA+GGG 0.505466 5.4:+34287096 None:intergenic
AAGAAAAGAAGGGTAATAAA+GGG 0.506609 5.4:+34287121 None:intergenic
GAATCCACTGTATATGTCAA+TGG 0.515896 5.4:-34286905 MS.gene99275:CDS
CTTACATTGGATTCCACCAC+CGG 0.521813 5.4:-34286590 MS.gene99275:CDS
CATGAGCAAAGGTACTAATT+GGG 0.521844 5.4:+34286006 None:intergenic
GTATATGGTGTTGTTGATAC+AGG 0.522915 5.4:-34286836 MS.gene99275:CDS
AGGAAATGAAACAGAATGAC+CGG 0.524965 5.4:+34286571 None:intergenic
GGGGCAACTTCCGGTATAGT+TGG 0.526062 5.4:-34286503 MS.gene99275:CDS
GCCGCTATGGTTTCTGGTGA+CGG 0.531191 5.4:-34286356 MS.gene99275:CDS
TAAGCCTAGTGATTGTATCA+AGG 0.531478 5.4:-34285865 MS.gene99275:CDS
AACAAAAGAATAGAATTTGG+AGG 0.531892 5.4:-34286257 MS.gene99275:CDS
AATTCCAAGACCAACTATAC+CGG 0.550046 5.4:+34286493 None:intergenic
ATAATACAGGGATGTTTCAA+GGG 0.555636 5.4:-34286523 MS.gene99275:CDS
AGAGTTCTGTTCATTACAAG+AGG 0.561935 5.4:+34286676 None:intergenic
ACAAAAGAATAGAATTTGGA+GGG 0.566114 5.4:-34286256 MS.gene99275:CDS
CTGATGTGATACTATAACAA+AGG 0.576754 5.4:+34286087 None:intergenic
TGAAGGATTAAATATTGGAG+TGG 0.577827 5.4:+34286760 None:intergenic
GGGTTAGATGACAGCGAGGA+GGG 0.580976 5.4:-34286236 MS.gene99275:CDS
GTGCAGCATTTACAACATGC+TGG 0.584049 5.4:+34286984 None:intergenic
ACAAAGGGAAAAGAAAAGAA+GGG 0.584352 5.4:+34287111 None:intergenic
CAATTGGAATCTTCAATTGG+TGG 0.584460 5.4:-34286449 MS.gene99275:CDS
TGGATTCAGGTGTATTAGAG+AGG 0.589556 5.4:+34286921 None:intergenic
TGACATAAGTTAGAAAAGCA+AGG 0.592506 5.4:+34286713 None:intergenic
TATTTGATGACTTATTCGGT+TGG 0.593557 5.4:-34286875 MS.gene99275:CDS
GGATTCAGGTGTATTAGAGA+GGG 0.619652 5.4:+34286922 None:intergenic
ACCATCAGCAACATGAGCAA+AGG 0.634882 5.4:+34285995 None:intergenic
TTGTTCGAGTCAACCATCAA+CGG 0.637876 5.4:+34286404 None:intergenic
TTATACTAATTTGGAATCGG+CGG 0.640658 5.4:-34286162 MS.gene99275:CDS
AAATTAGTATAAACATGAGA+CGG 0.651862 5.4:+34286173 None:intergenic
TACACCTTGATACAATCACT+AGG 0.657482 5.4:+34285861 None:intergenic
ACACCATATACATTAAAAGG+AGG 0.660614 5.4:+34286848 None:intergenic
ACCTCCATTGACATATACAG+TGG 0.662802 5.4:+34286901 None:intergenic
TAATACAGGGATGTTTCAAG+GGG 0.675676 5.4:-34286522 MS.gene99275:CDS
AGGGTTAGATGACAGCGAGG+AGG 0.678229 5.4:-34286237 MS.gene99275:CDS
AGTGATCAATCATATTCACA+AGG 0.687091 5.4:-34286632 MS.gene99275:CDS
GATGTGGACACAATAATACA+GGG 0.698577 5.4:-34286535 MS.gene99275:CDS
TCCACTGTATATGTCAATGG+AGG 0.710443 5.4:-34286902 MS.gene99275:CDS
ATGTTGTAAATGCTGCACGT+CGG 0.736684 5.4:-34286979 MS.gene99275:CDS
TGGAGGGTTAGATGACAGCG+AGG 0.736906 5.4:-34286240 MS.gene99275:CDS
AAATGAAACAGAATGACCGG+TGG 0.751744 5.4:+34286574 None:intergenic
ACCGTCACCAGAAACCATAG+CGG 0.784680 5.4:+34286355 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAGAAATAATGAAACAA+AGG + chr5.4:34285901-34285920 None:intergenic 15.0%
!! AAAAGAAATAATGAAACAAA+GGG + chr5.4:34285900-34285919 None:intergenic 15.0%
!! AAATTAGTATAAACATGAGA+CGG + chr5.4:34286823-34286842 None:intergenic 20.0%
!!! GATTTTGAAGGATTAAATAT+TGG + chr5.4:34286241-34286260 None:intergenic 20.0%
!!! TGTTTATACTAATTTGGAAT+CGG - chr5.4:34286828-34286847 MS.gene99275:CDS 20.0%
! AAAGAAAAGAAGGGTAATAA+AGG + chr5.4:34285876-34285895 None:intergenic 25.0%
! AACAAAAGAATAGAATTTGG+AGG - chr5.4:34286736-34286755 MS.gene99275:CDS 25.0%
! AAGAAAAGAAGGGTAATAAA+GGG + chr5.4:34285875-34285894 None:intergenic 25.0%
! ACAAAAGAATAGAATTTGGA+GGG - chr5.4:34286737-34286756 MS.gene99275:CDS 25.0%
! ACAACACCATATACATTAAA+AGG + chr5.4:34286151-34286170 None:intergenic 25.0%
! GGAAACAAAAGAATAGAATT+TGG - chr5.4:34286733-34286752 MS.gene99275:CDS 25.0%
! GGAATCTTGAAATAAATGAT+TGG + chr5.4:34286053-34286072 None:intergenic 25.0%
! GTCTCATGTTTATACTAATT+TGG - chr5.4:34286822-34286841 MS.gene99275:CDS 25.0%
! TCATTTCCTAAAACTGTTAT+CGG - chr5.4:34286436-34286455 MS.gene99275:CDS 25.0%
! TTAAAATGTGCAGTGATTAT+AGG + chr5.4:34286946-34286965 None:intergenic 25.0%
!! ATAATCACTGCACATTTTAA+AGG - chr5.4:34286946-34286965 MS.gene99275:CDS 25.0%
!! TGAGTATTTGATGACTTATT+CGG - chr5.4:34286114-34286133 MS.gene99275:CDS 25.0%
!!! TTGTAACTTGATGATTTTGA+AGG + chr5.4:34286253-34286272 None:intergenic 25.0%
AACAAAGGGAAAAGAAAAGA+AGG + chr5.4:34285886-34285905 None:intergenic 30.0%
AATAATACAGGGATGTTTCA+AGG - chr5.4:34286469-34286488 MS.gene99275:CDS 30.0%
AATTGTGTTGTAAGAGACAT+AGG + chr5.4:34286529-34286548 None:intergenic 30.0%
ACAAAGGGAAAAGAAAAGAA+GGG + chr5.4:34285885-34285904 None:intergenic 30.0%
ACACAATTGGAATCTTCAAT+TGG - chr5.4:34286541-34286560 MS.gene99275:CDS 30.0%
ACACCATATACATTAAAAGG+AGG + chr5.4:34286148-34286167 None:intergenic 30.0%
AGTGATCAATCATATTCACA+AGG - chr5.4:34286361-34286380 MS.gene99275:CDS 30.0%
ATAATACAGGGATGTTTCAA+GGG - chr5.4:34286470-34286489 MS.gene99275:CDS 30.0%
ATACAGGTAGTGACATTATT+TGG - chr5.4:34286173-34286192 MS.gene99275:CDS 30.0%
CAAAGGTTCAATAGTTGATT+AGG + chr5.4:34286892-34286911 None:intergenic 30.0%
CTGATGTGATACTATAACAA+AGG + chr5.4:34286909-34286928 None:intergenic 30.0%
TATGTCTCTTACAACACAAT+TGG - chr5.4:34286528-34286547 MS.gene99275:CDS 30.0%
TGAAGGATTAAATATTGGAG+TGG + chr5.4:34286236-34286255 None:intergenic 30.0%
TGACATAAGTTAGAAAAGCA+AGG + chr5.4:34286283-34286302 None:intergenic 30.0%
! ACACCTCCTTTTAATGTATA+TGG - chr5.4:34286142-34286161 MS.gene99275:CDS 30.0%
! TATTTGATGACTTATTCGGT+TGG - chr5.4:34286118-34286137 MS.gene99275:CDS 30.0%
! TTATACTAATTTGGAATCGG+CGG - chr5.4:34286831-34286850 MS.gene99275:CDS 30.0%
!! ACATTAGAAGCATTTAGTGT+TGG - chr5.4:34286712-34286731 MS.gene99275:CDS 30.0%
!!! TGAGAGTCTTTTTTCACAAA+AGG + chr5.4:34286676-34286695 None:intergenic 30.0%
AATTCCAAGACCAACTATAC+CGG + chr5.4:34286503-34286522 None:intergenic 35.0%
AGAGTTCTGTTCATTACAAG+AGG + chr5.4:34286320-34286339 None:intergenic 35.0%
AGGAAATGAAACAGAATGAC+CGG + chr5.4:34286425-34286444 None:intergenic 35.0%
CAATTGGAATCTTCAATTGG+TGG - chr5.4:34286544-34286563 MS.gene99275:CDS 35.0%
CTTGGCACAACTAAATTTGT+TGG - chr5.4:34287071-34287090 MS.gene99275:CDS 35.0%
GAATCCACTGTATATGTCAA+TGG - chr5.4:34286088-34286107 MS.gene99275:CDS 35.0%
GATGTGGACACAATAATACA+GGG - chr5.4:34286458-34286477 MS.gene99275:CDS 35.0%
GGTAACATCATACTTGATTC+AGG - chr5.4:34286778-34286797 MS.gene99275:CDS 35.0%
TAAATGATTGGCACGATTGA+TGG + chr5.4:34286041-34286060 None:intergenic 35.0%
TAAGCCTAGTGATTGTATCA+AGG - chr5.4:34287128-34287147 MS.gene99275:CDS 35.0%
TAATACAGGGATGTTTCAAG+GGG - chr5.4:34286471-34286490 MS.gene99275:CDS 35.0%
TACACCTTGATACAATCACT+AGG + chr5.4:34287135-34287154 None:intergenic 35.0%
! AAGACGAGCAAACTCAATTT+TGG - chr5.4:34286610-34286629 MS.gene99275:CDS 35.0%
! GCTTTTACATCATCTCAAAC+TGG - chr5.4:34287033-34287052 MS.gene99275:CDS 35.0%
! TAGTGTTGAGACTCTTACAT+TGG - chr5.4:34286390-34286409 MS.gene99275:CDS 35.0%
! TTGACATATACAGTGGATTC+AGG + chr5.4:34286088-34286107 None:intergenic 35.0%
!! ACATGAGCAAAGGTACTAAT+TGG + chr5.4:34286991-34287010 None:intergenic 35.0%
!! CATGAGCAAAGGTACTAATT+GGG + chr5.4:34286990-34287009 None:intergenic 35.0%
!! GCACAACTAAATTTGTTGGT+TGG - chr5.4:34287075-34287094 MS.gene99275:CDS 35.0%
!! GTATATGGTGTTGTTGATAC+AGG - chr5.4:34286157-34286176 MS.gene99275:CDS 35.0%
AAATGAAACAGAATGACCGG+TGG + chr5.4:34286422-34286441 None:intergenic 40.0%
ACCTCCATTGACATATACAG+TGG + chr5.4:34286095-34286114 None:intergenic 40.0%
ATGTTGTAAATGCTGCACGT+CGG - chr5.4:34286014-34286033 MS.gene99275:CDS 40.0%
CCTAAAACTGTTATCGGATG+TGG - chr5.4:34286442-34286461 MS.gene99275:CDS 40.0%
GGATGTGGACACAATAATAC+AGG - chr5.4:34286457-34286476 MS.gene99275:CDS 40.0%
GGATTCAGGTGTATTAGAGA+GGG + chr5.4:34286074-34286093 None:intergenic 40.0%
TCCACTGTATATGTCAATGG+AGG - chr5.4:34286091-34286110 MS.gene99275:CDS 40.0%
TGCTTGTAGCATTGTTCACA+AGG + chr5.4:34286211-34286230 None:intergenic 40.0%
TGGATTCAGGTGTATTAGAG+AGG + chr5.4:34286075-34286094 None:intergenic 40.0%
TTGTGCCAAGTTTCCAAAGA+TGG + chr5.4:34287061-34287080 None:intergenic 40.0%
TTGTTCGAGTCAACCATCAA+CGG + chr5.4:34286592-34286611 None:intergenic 40.0%
! CCACATCCGATAACAGTTTT+AGG + chr5.4:34286445-34286464 None:intergenic 40.0%
! TCTCAAACTGGTTCCATCTT+TGG - chr5.4:34287045-34287064 MS.gene99275:CDS 40.0%
! TGGTTCCATCTTTGGAAACT+TGG - chr5.4:34287053-34287072 MS.gene99275:CDS 40.0%
! TTACTGTTTGTTGCCGTTGA+TGG - chr5.4:34286576-34286595 MS.gene99275:CDS 40.0%
! TTTTGTGCAGGTTTGTAGAG+TGG + chr5.4:34285983-34286002 None:intergenic 40.0%
!! GGTATAGTTGGTCTTGGAAT+TGG - chr5.4:34286502-34286521 MS.gene99275:CDS 40.0%
!! GTGGTGATTTTGAAGAATCG+CGG + chr5.4:34285964-34285983 None:intergenic 40.0%
!!! TGCTGGAATTTGTTTTGTGC+AGG + chr5.4:34285995-34286014 None:intergenic 40.0%
ACCATCAGCAACATGAGCAA+AGG + chr5.4:34287001-34287020 None:intergenic 45.0%
ACCTTTGCTCATGTTGCTGA+TGG - chr5.4:34286997-34287016 MS.gene99275:CDS 45.0%
ATGTTTCAAGGGGCAACTTC+CGG - chr5.4:34286481-34286500 MS.gene99275:CDS 45.0%
CTTACATTGGATTCCACCAC+CGG - chr5.4:34286403-34286422 MS.gene99275:CDS 45.0%
GTGCAGCATTTACAACATGC+TGG + chr5.4:34286012-34286031 None:intergenic 45.0%
! ACTTCCGGTATAGTTGGTCT+TGG - chr5.4:34286496-34286515 MS.gene99275:CDS 45.0%
! TACTAATTTGGAATCGGCGG+TGG - chr5.4:34286834-34286853 MS.gene99275:CDS 45.0%
!! CAATTTTGGAGATGCCGCTA+TGG - chr5.4:34286624-34286643 MS.gene99275:CDS 45.0%
!! GTGATTTTGAAGAATCGCGG+TGG + chr5.4:34285961-34285980 None:intergenic 45.0%
ACCGTCACCAGAAACCATAG+CGG + chr5.4:34286641-34286660 None:intergenic 50.0%
TGAAACAGAATGACCGGTGG+TGG + chr5.4:34286419-34286438 None:intergenic 50.0%
! TGCTCATGTTGCTGATGGTG+TGG - chr5.4:34287002-34287021 MS.gene99275:CDS 50.0%
AGGGTTAGATGACAGCGAGG+AGG - chr5.4:34286756-34286775 MS.gene99275:CDS 55.0%
GGAGATGCCGCTATGGTTTC+TGG - chr5.4:34286631-34286650 MS.gene99275:CDS 55.0%
GGGGCAACTTCCGGTATAGT+TGG - chr5.4:34286490-34286509 MS.gene99275:CDS 55.0%
GGGTTAGATGACAGCGAGGA+GGG - chr5.4:34286757-34286776 MS.gene99275:CDS 55.0%
TGGAGGGTTAGATGACAGCG+AGG - chr5.4:34286753-34286772 MS.gene99275:CDS 55.0%
!! GCCGCTATGGTTTCTGGTGA+CGG - chr5.4:34286637-34286656 MS.gene99275:CDS 55.0%
GGAATCGGCGGTGGCACGAT+TGG - chr5.4:34286843-34286862 MS.gene99275:CDS 65.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 34285860 34287155 34285860 ID=MS.gene99275
chr5.4 mRNA 34285860 34287155 34285860 ID=MS.gene99275.t1;Parent=MS.gene99275
chr5.4 exon 34285860 34287155 34285860 ID=MS.gene99275.t1.exon1;Parent=MS.gene99275.t1
chr5.4 CDS 34285860 34287155 34285860 ID=cds.MS.gene99275.t1;Parent=MS.gene99275.t1
Gene Sequence

>MS.gene99275

ATGAATACTCGTCCCTTTATTACCCTTCTTTTCTTTTCCCTTTGTTTCATTATTTCTTTTTCTCATTCTCTACGCAATAGTTTTAGTTTTGAACTCATCCACCGCGATTCTTCAAAATCACCACTCTACAAACCTGCACAAAACAAATTCCAGCATGTTGTAAATGCTGCACGTCGGTCCATCAATCGTGCCAATCATTTATTTCAAGATTCCCTCTCTAATACACCTGAATCCACTGTATATGTCAATGGAGGTGAGTATTTGATGACTTATTCGGTTGGTACACCTCCTTTTAATGTATATGGTGTTGTTGATACAGGTAGTGACATTATTTGGCTTCAATGTAAGCCTTGTGAACAATGCTACAAGCAAACCACTCCAATATTTAATCCTTCAAAATCATCAAGTTACAAAAATATTCCTTGCTTTTCTAACTTATGTCAATCTGTGAGATATACCTCTTGTAATGAACAGAACTCTTGTGAATATACTATAAACTTTAGTGATCAATCATATTCACAAGGAGAACTTAGTGTTGAGACTCTTACATTGGATTCCACCACCGGTCATTCTGTTTCATTTCCTAAAACTGTTATCGGATGTGGACACAATAATACAGGGATGTTTCAAGGGGCAACTTCCGGTATAGTTGGTCTTGGAATTGGACCTATGTCTCTTACAACACAATTGGAATCTTCAATTGGTGGTAAATTTTCTTACTGTTTGTTGCCGTTGATGGTTGACTCGAACAAGACGAGCAAACTCAATTTTGGAGATGCCGCTATGGTTTCTGGTGACGGTGTTGTGTCAACTCCTTTTGTGAAAAAAGACTCTCAAGTTTTCTACTATTTGACATTAGAAGCATTTAGTGTTGGAAACAAAAGAATAGAATTTGGAGGGTTAGATGACAGCGAGGAGGGTAACATCATACTTGATTCAGGTACAACATTGACTCTTTTACCGTCTCATGTTTATACTAATTTGGAATCGGCGGTGGCACGATTGGTGAAACTAGATCGTGTTGATGATCCTAATCAACTATTGAACCTTTGTTATAGTATCACATCAGATCAATATGATTTTCCTATAATCACTGCACATTTTAAAGGTGCAGATATTAAGTTGAACCCAATTAGTACCTTTGCTCATGTTGCTGATGGTGTGGTTTGCTTAGCTTTTACATCATCTCAAACTGGTTCCATCTTTGGAAACTTGGCACAACTAAATTTGTTGGTTGGTTATGATCTTCAACAAAATATTGTGTCGTTTAAGCCTAGTGATTGTATCAAGGTGTAA
Protein sequence

>MS.gene99275.t1

MNTRPFITLLFFSLCFIISFSHSLRNSFSFELIHRDSSKSPLYKPAQNKFQHVVNAARRSINRANHLFQDSLSNTPESTVYVNGGEYLMTYSVGTPPFNVYGVVDTGSDIIWLQCKPCEQCYKQTTPIFNPSKSSSYKNIPCFSNLCQSVRYTSCNEQNSCEYTINFSDQSYSQGELSVETLTLDSTTGHSVSFPKTVIGCGHNNTGMFQGATSGIVGLGIGPMSLTTQLESSIGGKFSYCLLPLMVDSNKTSKLNFGDAAMVSGDGVVSTPFVKKDSQVFYYLTLEAFSVGNKRIEFGGLDDSEEGNIILDSGTTLTLLPSHVYTNLESAVARLVKLDRVDDPNQLLNLCYSITSDQYDFPIITAHFKGADIKLNPISTFAHVADGVVCLAFTSSQTGSIFGNLAQLNLLVGYDLQQNIVSFKPSDCIKV