Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99344.t1 | KEH16865.1 | 99.3 | 285 | 2 | 0 | 1 | 285 | 14 | 298 | 6.90E-160 | 573.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99344.t1 | P93286 | 84.5 | 251 | 31 | 4 | 1 | 247 | 14 | 260 | 1.4e-108 | 394.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99344.t1 | A0A072TTM0 | 99.3 | 285 | 2 | 0 | 1 | 285 | 14 | 298 | 5.0e-160 | 573.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050807 | MS.gene99344 | -0.802983 | 4.33E-49 | -1.69E-46 |
| MS.gene052239 | MS.gene99344 | 0.817491 | 3.21E-52 | -1.69E-46 |
| MS.gene053931 | MS.gene99344 | -0.805647 | 1.21E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99344.t1 | MTR_0082s0260 | 99.298 | 285 | 2 | 0 | 1 | 285 | 14 | 298 | 0.0 | 579 |
| MS.gene99344.t1 | MTR_0024s0140 | 98.268 | 231 | 4 | 0 | 55 | 285 | 1 | 231 | 2.64e-167 | 462 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99344.t1 | ATMG00180 | 86.056 | 251 | 27 | 4 | 1 | 247 | 14 | 260 | 1.17e-124 | 363 |
| MS.gene99344.t1 | AT2G07815 | 92.063 | 63 | 5 | 0 | 1 | 63 | 186 | 248 | 1.94e-31 | 118 |
Find 0 sgRNAs with CRISPR-Local
Find 126 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAGATGAATATTTCACATTT+TGG | + | 24089:13905-13924 | MS.gene99344:CDS | 20.0% |
| ! | ATTATGTCTGGTAATAGAAT+AGG | - | 24089:13428-13447 | MS.gene99344:intergenic | 25.0% |
| ! | ATTCTATTACCAGACATAAT+TGG | + | 24089:13428-13447 | MS.gene99344:CDS | 25.0% |
| ! | TGTGAAATATTCATCTTCAT+TGG | - | 24089:13902-13921 | MS.gene99344:intergenic | 25.0% |
| ! | TTATGTCTGGTAATAGAATA+GGG | - | 24089:13427-13446 | MS.gene99344:intergenic | 25.0% |
| ! | TTCTATTACCAGACATAATT+GGG | + | 24089:13429-13448 | MS.gene99344:CDS | 25.0% |
| !! | TCATATTTAGAATCTTTCTG+CGG | + | 24089:13542-13561 | MS.gene99344:CDS | 25.0% |
| AATAGGAATTATAGTCCCAT+TGG | - | 24089:13306-13325 | MS.gene99344:intergenic | 30.0% | |
| AATGTAGATAGACCAAAAGA+GGG | - | 24089:13343-13362 | MS.gene99344:intergenic | 30.0% | |
| ACGAACCATTTCAATAGTAA+GGG | - | 24089:13251-13270 | MS.gene99344:intergenic | 30.0% | |
| ATAAGAAGGAAATGAAGAAC+GGG | - | 24089:13503-13522 | MS.gene99344:intergenic | 30.0% | |
| ATTATAGTCCCATTGGAAAA+AGG | - | 24089:13299-13318 | MS.gene99344:intergenic | 30.0% | |
| GATAAGAAGGAAATGAAGAA+CGG | - | 24089:13504-13523 | MS.gene99344:intergenic | 30.0% | |
| TTATAGTCCCATTGGAAAAA+GGG | - | 24089:13298-13317 | MS.gene99344:intergenic | 30.0% | |
| AACAACGAAATGACAAGATG+GGG | + | 24089:13687-13706 | MS.gene99344:CDS | 35.0% | |
| CAACAACGAAATGACAAGAT+GGG | + | 24089:13686-13705 | MS.gene99344:CDS | 35.0% | |
| GAATGTAGATAGACCAAAAG+AGG | - | 24089:13344-13363 | MS.gene99344:intergenic | 35.0% | |
| GACGAACCATTTCAATAGTA+AGG | - | 24089:13252-13271 | MS.gene99344:intergenic | 35.0% | |
| TATAGTCCCATTGGAAAAAG+GGG | - | 24089:13297-13316 | MS.gene99344:intergenic | 35.0% | |
| TTCCTTCTTATCGAATCCAA+GGG | + | 24089:13512-13531 | MS.gene99344:CDS | 35.0% | |
| TTCGACCCTTCTTTCTAAAT+GGG | - | 24089:13726-13745 | MS.gene99344:intergenic | 35.0% | |
| TTTCCTTCTTATCGAATCCA+AGG | + | 24089:13511-13530 | MS.gene99344:CDS | 35.0% | |
| ! | TCATCTTCATTGGAGCTTTT+TGG | - | 24089:13892-13911 | MS.gene99344:intergenic | 35.0% |
| ! | TTTTGGTCTATCTACATTCC+AGG | + | 24089:13345-13364 | MS.gene99344:CDS | 35.0% |
| AAATTCATACGCTCCATGGA+CGG | + | 24089:13368-13387 | MS.gene99344:CDS | 40.0% | |
| AGACCAAAAGAGGGAATGAA+GGG | - | 24089:13334-13353 | MS.gene99344:intergenic | 40.0% | |
| CAGCACCCTTACTATTGAAA+TGG | + | 24089:13243-13262 | MS.gene99344:CDS | 40.0% | |
| CATTTAGAAAGAAGGGTCGA+AGG | + | 24089:13725-13744 | MS.gene99344:CDS | 40.0% | |
| CTTCGACCCTTCTTTCTAAA+TGG | - | 24089:13727-13746 | MS.gene99344:intergenic | 40.0% | |
| GAAGGATACACCAAAGACTT+AGG | + | 24089:13642-13661 | MS.gene99344:CDS | 40.0% | |
| GCAACAACGAAATGACAAGA+TGG | + | 24089:13685-13704 | MS.gene99344:CDS | 40.0% | |
| GGGAATGAAGGGATAAGAAT+AGG | - | 24089:13323-13342 | MS.gene99344:intergenic | 40.0% | |
| TAAATATGAGAAGTCCCCCT+TGG | - | 24089:13531-13550 | MS.gene99344:intergenic | 40.0% | |
| TAGACCAAAAGAGGGAATGA+AGG | - | 24089:13335-13354 | MS.gene99344:intergenic | 40.0% | |
| TCCTTCTTATCGAATCCAAG+GGG | + | 24089:13513-13532 | MS.gene99344:CDS | 40.0% | |
| TCGACCCTTCTTTCTAAATG+GGG | - | 24089:13725-13744 | MS.gene99344:intergenic | 40.0% | |
| TGTCTGGTAATAGAATAGGG+CGG | - | 24089:13424-13443 | MS.gene99344:intergenic | 40.0% | |
| ! | AAAGAAGGGTCGAAGGTTTT+GGG | + | 24089:13732-13751 | MS.gene99344:CDS | 40.0% |
| ! | TATCCCTTCATTCCCTCTTT+TGG | + | 24089:13328-13347 | MS.gene99344:CDS | 40.0% |
| ! | TCTTCATTGGAGCTTTTTGG+TGG | - | 24089:13889-13908 | MS.gene99344:intergenic | 40.0% |
| AACGGGAACGAAACGAAATG+AGG | - | 24089:13486-13505 | MS.gene99344:intergenic | 45.0% | |
| AGACTTAGGCCTAACCCTAA+CGG | + | 24089:13656-13675 | MS.gene99344:CDS | 45.0% | |
| CAGGAAATTCATACGCTCCA+TGG | + | 24089:13364-13383 | MS.gene99344:CDS | 45.0% | |
| CATGGAGCGTATGAATTTCC+TGG | - | 24089:13366-13385 | MS.gene99344:intergenic | 45.0% | |
| CCTTCTTATCGAATCCAAGG+GGG | + | 24089:13514-13533 | MS.gene99344:CDS | 45.0% | |
| CGACCCTTCTTTCTAAATGG+GGG | - | 24089:13724-13743 | MS.gene99344:intergenic | 45.0% | |
| GAGCTTCTCCCAATTATGTC+TGG | - | 24089:13440-13459 | MS.gene99344:intergenic | 45.0% | |
| GCACCCCCATTTAGAAAGAA+GGG | + | 24089:13718-13737 | MS.gene99344:CDS | 45.0% | |
| GGAGTGTGCATCTGTATGTT+GGG | + | 24089:13926-13945 | MS.gene99344:CDS | 45.0% | |
| GTTGTTGCTCATTCCCGTTA+GGG | - | 24089:13673-13692 | MS.gene99344:intergenic | 45.0% | |
| TAGGGTTAGGCCTAAGTCTT+TGG | - | 24089:13655-13674 | MS.gene99344:intergenic | 45.0% | |
| TCTTCTCTTTACCACGCGAT+AGG | + | 24089:13594-13613 | MS.gene99344:CDS | 45.0% | |
| TGGAGTGTGCATCTGTATGT+TGG | + | 24089:13925-13944 | MS.gene99344:CDS | 45.0% | |
| ! | GAAAGAAGGGTCGAAGGTTT+TGG | + | 24089:13731-13750 | MS.gene99344:CDS | 45.0% |
| AGAGCTTCAAGCCCGATTTC+AGG | - | 24089:13821-13840 | MS.gene99344:intergenic | 50.0% | |
| AGCTCATCCGAAACTAGAGC+TGG | + | 24089:13455-13474 | MS.gene99344:CDS | 50.0% | |
| ATAGGTCAGCAAAGCGTGAG+CGG | + | 24089:13612-13631 | MS.gene99344:CDS | 50.0% | |
| CCCCCTTGGATTCGATAAGA+AGG | - | 24089:13517-13536 | MS.gene99344:intergenic | 50.0% | |
| CGGAGCAAAAAGTGGAGTCT+TGG | + | 24089:13388-13407 | MS.gene99344:CDS | 50.0% | |
| CGTTGTTGCTCATTCCCGTT+AGG | - | 24089:13674-13693 | MS.gene99344:intergenic | 50.0% | |
| GACTTAGGCCTAACCCTAAC+GGG | + | 24089:13657-13676 | MS.gene99344:CDS | 50.0% | |
| GGCACCCCCATTTAGAAAGA+AGG | + | 24089:13717-13736 | MS.gene99344:CDS | 50.0% | |
| GTCAGTAGAGATGTTCCCAC+AGG | + | 24089:13269-13288 | MS.gene99344:CDS | 50.0% | |
| GTGCCACCTGAACCTGAAAT+CGG | + | 24089:13806-13825 | MS.gene99344:CDS | 50.0% | |
| TCCATGGACGGAGCAAAAAG+TGG | + | 24089:13380-13399 | MS.gene99344:CDS | 50.0% | |
| TGCCACCTGAACCTGAAATC+GGG | + | 24089:13807-13826 | MS.gene99344:CDS | 50.0% | |
| TGCTCATTCCCGTTAGGGTT+AGG | - | 24089:13668-13687 | MS.gene99344:intergenic | 50.0% | |
| ! | AATGACAAGATGGGGTGCTC+CGG | + | 24089:13695-13714 | MS.gene99344:CDS | 50.0% |
| ! | ACAGGTGCCCCTTTTTCCAA+TGG | + | 24089:13287-13306 | MS.gene99344:CDS | 50.0% |
| ! | CAGGTGCCCCTTTTTCCAAT+GGG | + | 24089:13288-13307 | MS.gene99344:CDS | 50.0% |
| ! | GGCGTTTCCAGCTCTAGTTT+CGG | - | 24089:13465-13484 | MS.gene99344:intergenic | 50.0% |
| !! | ATTGGAAAAAGGGGCACCTG+TGG | - | 24089:13288-13307 | MS.gene99344:intergenic | 50.0% |
| !! | TCAAGCCCGATTTCAGGTTC+AGG | - | 24089:13815-13834 | MS.gene99344:intergenic | 50.0% |
| !! | TCTTTCTAAATGGGGGTGCC+CGG | - | 24089:13717-13736 | MS.gene99344:intergenic | 50.0% |
| !! | TTGGAAAAAGGGGCACCTGT+GGG | - | 24089:13287-13306 | MS.gene99344:intergenic | 50.0% |
| !!! | TCCACTTTTTGCTCCGTCCA+TGG | - | 24089:13384-13403 | MS.gene99344:intergenic | 50.0% |
| CCACGCCTCCTATTTGTTCG+GGG | + | 24089:13994-14013 | MS.gene99344:CDS | 55.0% | |
| CCCACGCCTCCTATTTGTTC+GGG | + | 24089:13993-14012 | MS.gene99344:CDS | 55.0% | |
| GACCAACAGCCATCAGCTGT+CGG | - | 24089:13859-13878 | MS.gene99344:intergenic | 55.0% | |
| GCCCACGCCTCCTATTTGTT+CGG | + | 24089:13992-14011 | MS.gene99344:CDS | 55.0% | |
| TAGGTCAGCAAAGCGTGAGC+GGG | + | 24089:13613-13632 | MS.gene99344:CDS | 55.0% | |
| TCAACTGAGCATCTCAGCGG+CGG | - | 24089:14059-14078 | MS.gene99344:intergenic | 55.0% | |
| TGTTCTTCTGTCGTGCGACC+CGG | + | 24089:13949-13968 | MS.gene99344:CDS | 55.0% | |
| ! | ATGACAAGATGGGGTGCTCC+GGG | + | 24089:13696-13715 | MS.gene99344:CDS | 55.0% |
| ! | CCCGAACAAATAGGAGGCGT+GGG | - | 24089:13996-14015 | MS.gene99344:intergenic | 55.0% |
| ! | CGTGAACTCTGACTCGATCC+GGG | + | 24089:14024-14043 | MS.gene99344:CDS | 55.0% |
| ! | GCGTGAACTCTGACTCGATC+CGG | + | 24089:14023-14042 | MS.gene99344:CDS | 55.0% |
| !! | AGCCCGATTTCAGGTTCAGG+TGG | - | 24089:13812-13831 | MS.gene99344:intergenic | 55.0% |
| ACAAGCACCACCCGACGAAG+GGG | - | 24089:13777-13796 | MS.gene99344:intergenic | 60.0% | |
| ACACAAGCACCACCCGACGA+AGG | - | 24089:13779-13798 | MS.gene99344:intergenic | 60.0% | |
| ACAGGTCGCACATAAGCCGC+CGG | - | 24089:13971-13990 | MS.gene99344:intergenic | 60.0% | |
| ACCAACGAGCCGACAGCTGA+TGG | + | 24089:13847-13866 | MS.gene99344:CDS | 60.0% | |
| AGCCGACAGCTGATGGCTGT+TGG | + | 24089:13854-13873 | MS.gene99344:CDS | 60.0% | |
| CAACTGAGCATCTCAGCGGC+GGG | - | 24089:14058-14077 | MS.gene99344:intergenic | 60.0% | |
| CACAAGCACCACCCGACGAA+GGG | - | 24089:13778-13797 | MS.gene99344:intergenic | 60.0% | |
| CAGCGGCGGGATTGAATACC+CGG | - | 24089:14045-14064 | MS.gene99344:intergenic | 60.0% | |
| CCCCGAACAAATAGGAGGCG+TGG | - | 24089:13997-14016 | MS.gene99344:intergenic | 60.0% | |
| CGCCTCCTATTTGTTCGGGG+CGG | + | 24089:13997-14016 | MS.gene99344:CDS | 60.0% | |
| CGCTTTGCTGACCTATCGCG+TGG | - | 24089:13608-13627 | MS.gene99344:intergenic | 60.0% | |
| GCCTCCTATTTGTTCGGGGC+GGG | + | 24089:13998-14017 | MS.gene99344:CDS | 60.0% | |
| TCCTATTTGTTCGGGGCGGG+CGG | + | 24089:14001-14020 | MS.gene99344:CDS | 60.0% | |
| TCGTCGGGTGGTGCTTGTGT+GGG | + | 24089:13779-13798 | MS.gene99344:CDS | 60.0% | |
| TTCGTCGGGTGGTGCTTGTG+TGG | + | 24089:13778-13797 | MS.gene99344:CDS | 60.0% | |
| ! | AAATAGGAGGCGTGGGCCAC+AGG | - | 24089:13989-14008 | MS.gene99344:intergenic | 60.0% |
| !! | CAGCTGTCGGCTCGTTGGTA+AGG | - | 24089:13846-13865 | MS.gene99344:intergenic | 60.0% |
| AAGCACCACCCGACGAAGGG+GGG | - | 24089:13775-13794 | MS.gene99344:intergenic | 65.0% | |
| CAAGCACCACCCGACGAAGG+GGG | - | 24089:13776-13795 | MS.gene99344:intergenic | 65.0% | |
| CAGGTCGCACATAAGCCGCC+GGG | - | 24089:13970-13989 | MS.gene99344:intergenic | 65.0% | |
| CGTCGGGTGGTGCTTGTGTG+GGG | + | 24089:13780-13799 | MS.gene99344:CDS | 65.0% | |
| GCCCGCCCCGAACAAATAGG+AGG | - | 24089:14002-14021 | MS.gene99344:intergenic | 65.0% | |
| GGCGGCTTATGTGCGACCTG+TGG | + | 24089:13970-13989 | MS.gene99344:CDS | 65.0% | |
| GTCGGGTGGTGCTTGTGTGG+GGG | + | 24089:13781-13800 | MS.gene99344:CDS | 65.0% | |
| TCGGGTGGTGCTTGTGTGGG+GGG | + | 24089:13782-13801 | MS.gene99344:CDS | 65.0% | |
| TCTTCTGTCGTGCGACCCGG+CGG | + | 24089:13952-13971 | MS.gene99344:CDS | 65.0% | |
| TTTCCCCGTCCCCCCTTCGT+CGG | + | 24089:13763-13782 | MS.gene99344:CDS | 65.0% | |
| !! | GCCATCAGCTGTCGGCTCGT+TGG | - | 24089:13851-13870 | MS.gene99344:intergenic | 65.0% |
| ACCACCCGACGAAGGGGGGA+CGG | - | 24089:13771-13790 | MS.gene99344:intergenic | 70.0% | |
| CAGCAAAGCGTGAGCGGGCG+CGG | + | 24089:13618-13637 | MS.gene99344:CDS | 70.0% | |
| GCCGCCCGCCCCGAACAAAT+AGG | - | 24089:14005-14024 | MS.gene99344:intergenic | 70.0% | |
| GGGGGGACGGGGAAAGCTAC+AGG | - | 24089:13758-13777 | MS.gene99344:intergenic | 70.0% | |
| TTCCCCGTCCCCCCTTCGTC+GGG | + | 24089:13764-13783 | MS.gene99344:CDS | 70.0% | |
| !! | AAGGGTGCTGCCGTGCCACG+AGG | - | 24089:13233-13252 | MS.gene99344:intergenic | 70.0% |
| AGCGTGAGCGGGCGCGGAGA+AGG | + | 24089:13624-13643 | MS.gene99344:CDS | 75.0% | |
| CACCCGACGAAGGGGGGACG+GGG | - | 24089:13769-13788 | MS.gene99344:intergenic | 75.0% | |
| CCACCCGACGAAGGGGGGAC+GGG | - | 24089:13770-13789 | MS.gene99344:intergenic | 75.0% | |
| ! | CCCGTCCCCCCTTCGTCGGG+TGG | + | 24089:13767-13786 | MS.gene99344:CDS | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 24089 | gene | 13221 | 14078 | 13221 | ID=MS.gene99344 |
| 24089 | mRNA | 13221 | 14078 | 13221 | ID=MS.gene99344.t1;Parent=MS.gene99344 |
| 24089 | exon | 13221 | 14078 | 13221 | ID=MS.gene99344.t1.exon1;Parent=MS.gene99344.t1 |
| 24089 | CDS | 13221 | 14078 | 13221 | ID=cds.MS.gene99344.t1;Parent=MS.gene99344.t1 |
| Gene Sequence |
| Protein sequence |
