Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99429.t1 | XP_013459371.1 | 98.8 | 160 | 2 | 0 | 1 | 160 | 1 | 160 | 5.30E-85 | 323.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99429.t1 | P24922 | 95.0 | 159 | 8 | 0 | 1 | 159 | 1 | 159 | 5.6e-83 | 308.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99429.t1 | I3T5P6 | 98.8 | 160 | 2 | 0 | 1 | 160 | 1 | 160 | 3.8e-85 | 323.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene054559 | MS.gene99429 | -0.803459 | 3.45E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99429.t1 | MTR_3g437680 | 98.750 | 160 | 2 | 0 | 1 | 160 | 1 | 160 | 4.28e-117 | 328 |
| MS.gene99429.t1 | MTR_3g437680 | 98.750 | 160 | 2 | 0 | 1 | 160 | 1 | 160 | 4.28e-117 | 328 |
| MS.gene99429.t1 | MTR_4g109130 | 96.250 | 160 | 6 | 0 | 1 | 160 | 1 | 160 | 1.56e-112 | 316 |
| MS.gene99429.t1 | MTR_5g042990 | 92.453 | 159 | 12 | 0 | 1 | 159 | 1 | 159 | 1.91e-108 | 306 |
| MS.gene99429.t1 | MTR_4g130917 | 92.453 | 159 | 12 | 0 | 1 | 159 | 1 | 159 | 3.94e-108 | 305 |
| MS.gene99429.t1 | MTR_5g042990 | 93.701 | 127 | 8 | 0 | 1 | 127 | 1 | 127 | 3.11e-87 | 251 |
| MS.gene99429.t1 | MTR_5g042990 | 87.681 | 138 | 17 | 0 | 1 | 138 | 1 | 138 | 3.63e-87 | 251 |
| MS.gene99429.t1 | MTR_4g130917 | 92.126 | 127 | 10 | 0 | 1 | 127 | 1 | 127 | 7.52e-86 | 248 |
| MS.gene99429.t1 | MTR_4g109130 | 88.333 | 120 | 14 | 0 | 1 | 120 | 1 | 120 | 4.60e-74 | 218 |
| MS.gene99429.t1 | MTR_4g112440 | 71.569 | 102 | 19 | 1 | 1 | 102 | 1 | 92 | 5.45e-48 | 150 |
| MS.gene99429.t1 | MTR_4g130907 | 46.875 | 128 | 20 | 2 | 18 | 145 | 42 | 121 | 5.21e-26 | 96.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99429.t1 | AT1G13950 | 92.453 | 159 | 11 | 1 | 1 | 159 | 1 | 158 | 6.68e-107 | 302 |
| MS.gene99429.t1 | AT1G69410 | 86.792 | 159 | 20 | 1 | 1 | 159 | 1 | 158 | 1.91e-100 | 285 |
| MS.gene99429.t1 | AT1G26630 | 80.892 | 157 | 29 | 1 | 1 | 157 | 1 | 156 | 4.63e-93 | 267 |
| MS.gene99429.t1 | AT1G26630 | 83.465 | 127 | 20 | 1 | 1 | 127 | 1 | 126 | 1.96e-76 | 224 |
Find 39 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTTTGGACCAATATCTTTC+AGG | 0.187453 | 3.4:-29179549 | None:intergenic |
| AATAGTTGAAGCTAGTTCTT+TGG | 0.241243 | 3.4:-29179565 | None:intergenic |
| GTGAGTCTGCTTACTGAAAA+TGG | 0.350641 | 3.4:+29177927 | MS.gene99429:CDS |
| TTGATCGATATTTCTGAAGA+TGG | 0.379975 | 3.4:+29177797 | MS.gene99429:CDS |
| CTTGGCATGACCATGCTTAC+CGG | 0.382469 | 3.4:-29177580 | None:intergenic |
| ATTAATGCCCTGAAAGATAT+TGG | 0.418980 | 3.4:+29179541 | MS.gene99429:CDS |
| ACCATGCTTACCGGTCTTTG+AGG | 0.422545 | 3.4:-29177571 | None:intergenic |
| CTTTGGACCAATATCTTTCA+GGG | 0.429224 | 3.4:-29179548 | None:intergenic |
| CATGCCAAGTGTCACTTTGT+TGG | 0.465747 | 3.4:+29177594 | MS.gene99429:CDS |
| TCTTGTATGGCAGGTTGTTG+AGG | 0.496646 | 3.4:+29177542 | MS.gene99429:intron |
| TCAACGTACATCACAATTGT+GGG | 0.508011 | 3.4:-29177664 | None:intergenic |
| CGGATGGTTCCGGCTTGTTG+AGG | 0.515178 | 3.4:-29171831 | None:intergenic |
| ATCAACGTACATCACAATTG+TGG | 0.520978 | 3.4:-29177665 | None:intergenic |
| TGTGTCCGTGATGTCTGCTA+TGG | 0.523316 | 3.4:+29179507 | MS.gene99429:CDS |
| AGGATAAGTCTTGGAAGCTC+CGG | 0.527718 | 3.4:-29171811 | None:intergenic |
| TACATCACAATTGTGGGAAG+AGG | 0.536885 | 3.4:-29177658 | None:intergenic |
| GGCTTGTTGAGGATAAGTCT+TGG | 0.549527 | 3.4:-29171820 | None:intergenic |
| ACTCATCATCATGTCGGACG+AGG | 0.557318 | 3.4:+29171761 | None:intergenic |
| TTTGAGTCAAAAGCCGATGC+CGG | 0.561371 | 3.4:+29171792 | MS.gene99429:CDS |
| AGGAAGCTTCAAGTCATCCT+TGG | 0.562183 | 3.4:-29177955 | None:intergenic |
| GTGTCCGTGATGTCTGCTAT+GGG | 0.564696 | 3.4:+29179508 | MS.gene99429:CDS |
| TGGTCACTGCAGATCAAGGA+AGG | 0.565385 | 3.4:+29179460 | MS.gene99429:intron |
| AGTCTTGGAAGCTCCGGCAT+CGG | 0.567163 | 3.4:-29171805 | None:intergenic |
| GAGGTTTCAACCTCAAAGAC+CGG | 0.568619 | 3.4:+29177561 | MS.gene99429:CDS |
| ACCTCAAAGACCGGTAAGCA+TGG | 0.571121 | 3.4:+29177570 | MS.gene99429:CDS |
| CTGAGTAAGCAGATTGTCAT+CGG | 0.573674 | 3.4:-29177979 | None:intergenic |
| CGATGACAATCTGCTTACTC+AGG | 0.577063 | 3.4:+29177980 | MS.gene99429:CDS |
| CGTGATGTCTGCTATGGGTG+AGG | 0.597852 | 3.4:+29179513 | MS.gene99429:CDS |
| ACATCACAATTGTGGGAAGA+GGG | 0.598510 | 3.4:-29177657 | None:intergenic |
| CAGACATCACGGACACAACA+AGG | 0.601971 | 3.4:-29179501 | None:intergenic |
| AATATCGATCAATTGATAGT+CGG | 0.622059 | 3.4:-29177786 | None:intergenic |
| AAGACTTATCCTCAACAAGC+CGG | 0.629507 | 3.4:+29171822 | MS.gene99429:CDS |
| GATAGTCGGTACGATTCACA+TGG | 0.640579 | 3.4:-29177772 | None:intergenic |
| GATTCCAACAAAGTGACACT+TGG | 0.646223 | 3.4:-29177598 | None:intergenic |
| CTCACCCATAGCAGACATCA+CGG | 0.658249 | 3.4:-29179512 | None:intergenic |
| GTAAGCAGATTGTCATCGGT+AGG | 0.661464 | 3.4:-29177975 | None:intergenic |
| TACTGAAAATGGTAACACCA+AGG | 0.675504 | 3.4:+29177938 | MS.gene99429:CDS |
| ATAGTCGGTACGATTCACAT+GGG | 0.694380 | 3.4:-29177771 | None:intergenic |
| TAGTCGGTACGATTCACATG+GGG | 0.757208 | 3.4:-29177770 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 29171771 | 29179576 | 29171771 | ID=MS.gene99429 |
| chr3.4 | mRNA | 29171771 | 29179576 | 29171771 | ID=MS.gene99429.t1;Parent=MS.gene99429 |
| chr3.4 | exon | 29171771 | 29171893 | 29171771 | ID=MS.gene99429.t1.exon1;Parent=MS.gene99429.t1 |
| chr3.4 | CDS | 29171771 | 29171893 | 29171771 | ID=cds.MS.gene99429.t1;Parent=MS.gene99429.t1 |
| chr3.4 | exon | 29177555 | 29177677 | 29177555 | ID=MS.gene99429.t1.exon2;Parent=MS.gene99429.t1 |
| chr3.4 | CDS | 29177555 | 29177677 | 29177555 | ID=cds.MS.gene99429.t1;Parent=MS.gene99429.t1 |
| chr3.4 | exon | 29177767 | 29177823 | 29177767 | ID=MS.gene99429.t1.exon3;Parent=MS.gene99429.t1 |
| chr3.4 | CDS | 29177767 | 29177823 | 29177767 | ID=cds.MS.gene99429.t1;Parent=MS.gene99429.t1 |
| chr3.4 | exon | 29177927 | 29178001 | 29177927 | ID=MS.gene99429.t1.exon4;Parent=MS.gene99429.t1 |
| chr3.4 | CDS | 29177927 | 29178001 | 29177927 | ID=cds.MS.gene99429.t1;Parent=MS.gene99429.t1 |
| chr3.4 | exon | 29179472 | 29179576 | 29179472 | ID=MS.gene99429.t1.exon5;Parent=MS.gene99429.t1 |
| chr3.4 | CDS | 29179472 | 29179576 | 29179472 | ID=cds.MS.gene99429.t1;Parent=MS.gene99429.t1 |
| Gene Sequence |
| Protein sequence |
