Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99631.t1 | XP_003592422.1 | 94.6 | 185 | 10 | 0 | 1 | 185 | 1 | 185 | 1.90E-73 | 285.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99631.t1 | P80728 | 54.5 | 101 | 46 | 0 | 22 | 122 | 1 | 101 | 1.8e-29 | 130.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99631.t1 | G7ID83 | 94.6 | 185 | 10 | 0 | 1 | 185 | 1 | 185 | 1.3e-73 | 285.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05777 | MS.gene99631 | 0.815398 | 9.43E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99631.t1 | MTR_1g104800 | 96.757 | 185 | 6 | 0 | 1 | 185 | 1 | 185 | 7.43e-118 | 332 |
| MS.gene99631.t1 | MTR_5g006040 | 70.909 | 165 | 41 | 3 | 1 | 164 | 1 | 159 | 4.41e-85 | 249 |
| MS.gene99631.t1 | MTR_4g114870 | 39.264 | 163 | 89 | 3 | 1 | 157 | 1 | 159 | 4.42e-27 | 105 |
| MS.gene99631.t1 | MTR_4g114870 | 46.000 | 100 | 52 | 1 | 25 | 122 | 191 | 290 | 5.66e-27 | 105 |
| MS.gene99631.t1 | MTR_8g007020 | 33.125 | 160 | 88 | 6 | 12 | 161 | 11 | 161 | 4.81e-27 | 101 |
| MS.gene99631.t1 | MTR_2g088990 | 37.013 | 154 | 81 | 6 | 8 | 148 | 6 | 156 | 4.57e-24 | 94.7 |
| MS.gene99631.t1 | MTR_7g086280 | 36.571 | 175 | 104 | 4 | 1 | 173 | 1 | 170 | 1.96e-23 | 91.7 |
| MS.gene99631.t1 | MTR_7g086230 | 38.636 | 176 | 99 | 5 | 1 | 173 | 1 | 170 | 4.18e-23 | 90.9 |
| MS.gene99631.t1 | MTR_1g105130 | 38.017 | 121 | 71 | 3 | 6 | 124 | 5 | 123 | 9.39e-23 | 89.4 |
| MS.gene99631.t1 | MTR_1g105120 | 38.017 | 121 | 71 | 3 | 6 | 124 | 5 | 123 | 9.39e-23 | 89.4 |
| MS.gene99631.t1 | MTR_7g086160 | 38.095 | 126 | 73 | 4 | 1 | 123 | 1 | 124 | 1.63e-22 | 89.0 |
| MS.gene99631.t1 | MTR_3g099570 | 36.364 | 143 | 84 | 5 | 5 | 145 | 4 | 141 | 2.00e-22 | 89.4 |
| MS.gene99631.t1 | MTR_1g090420 | 31.646 | 158 | 106 | 2 | 7 | 162 | 9 | 166 | 2.04e-22 | 90.1 |
| MS.gene99631.t1 | MTR_6g083240 | 33.333 | 183 | 108 | 6 | 1 | 176 | 1 | 176 | 3.01e-22 | 88.6 |
| MS.gene99631.t1 | MTR_7g086140 | 36.800 | 125 | 75 | 3 | 1 | 123 | 1 | 123 | 3.01e-22 | 88.2 |
| MS.gene99631.t1 | MTR_7g086190 | 36.800 | 125 | 75 | 3 | 1 | 123 | 1 | 123 | 3.01e-22 | 88.2 |
| MS.gene99631.t1 | MTR_8g007035 | 36.842 | 114 | 67 | 4 | 12 | 123 | 11 | 121 | 3.15e-22 | 89.4 |
| MS.gene99631.t1 | MTR_3g099580 | 42.574 | 101 | 55 | 3 | 22 | 122 | 23 | 120 | 3.56e-22 | 88.6 |
| MS.gene99631.t1 | MTR_2g101300 | 34.266 | 143 | 84 | 4 | 19 | 158 | 21 | 156 | 3.74e-22 | 89.0 |
| MS.gene99631.t1 | MTR_2g043730 | 37.168 | 113 | 66 | 2 | 9 | 121 | 16 | 123 | 6.58e-22 | 86.3 |
| MS.gene99631.t1 | MTR_4g078410 | 29.747 | 158 | 107 | 1 | 22 | 175 | 22 | 179 | 2.69e-21 | 86.7 |
| MS.gene99631.t1 | MTR_3g089005 | 38.393 | 112 | 64 | 2 | 10 | 121 | 18 | 124 | 3.68e-21 | 84.3 |
| MS.gene99631.t1 | MTR_1g090190 | 34.091 | 132 | 80 | 4 | 22 | 151 | 20 | 146 | 8.92e-21 | 85.1 |
| MS.gene99631.t1 | MTR_7g086220 | 35.043 | 117 | 72 | 3 | 1 | 116 | 1 | 114 | 2.89e-20 | 83.2 |
| MS.gene99631.t1 | MTR_7g086100 | 41.045 | 134 | 70 | 4 | 7 | 140 | 14 | 138 | 3.67e-20 | 84.3 |
| MS.gene99631.t1 | MTR_1g014120 | 36.893 | 103 | 63 | 1 | 43 | 145 | 46 | 146 | 6.31e-20 | 82.8 |
| MS.gene99631.t1 | MTR_8g089110 | 32.456 | 114 | 72 | 2 | 8 | 121 | 11 | 119 | 6.55e-20 | 81.3 |
| MS.gene99631.t1 | MTR_3g099680 | 36.667 | 120 | 71 | 4 | 6 | 122 | 5 | 122 | 7.85e-20 | 83.6 |
| MS.gene99631.t1 | MTR_1g077790 | 35.976 | 164 | 99 | 4 | 25 | 183 | 29 | 191 | 1.07e-19 | 82.4 |
| MS.gene99631.t1 | MTR_3g099980 | 36.667 | 120 | 71 | 4 | 6 | 122 | 5 | 122 | 1.48e-19 | 82.8 |
| MS.gene99631.t1 | MTR_3g089035 | 39.394 | 99 | 55 | 3 | 22 | 120 | 30 | 123 | 2.52e-19 | 79.7 |
| MS.gene99631.t1 | MTR_6g023760 | 35.294 | 102 | 62 | 3 | 22 | 122 | 23 | 121 | 2.68e-19 | 80.9 |
| MS.gene99631.t1 | MTR_8g086360 | 28.333 | 180 | 115 | 4 | 8 | 173 | 7 | 186 | 4.81e-19 | 80.9 |
| MS.gene99631.t1 | MTR_1g112700 | 32.800 | 125 | 77 | 3 | 7 | 126 | 11 | 133 | 6.13e-19 | 80.1 |
| MS.gene99631.t1 | MTR_3g099540 | 34.677 | 124 | 76 | 4 | 1 | 122 | 1 | 121 | 9.51e-19 | 78.6 |
| MS.gene99631.t1 | MTR_8g088830 | 35.514 | 107 | 59 | 4 | 18 | 121 | 21 | 120 | 1.46e-18 | 77.8 |
| MS.gene99631.t1 | MTR_3g105930 | 30.935 | 139 | 81 | 3 | 43 | 178 | 48 | 174 | 1.90e-18 | 79.0 |
| MS.gene99631.t1 | MTR_2g079030 | 28.144 | 167 | 114 | 2 | 1 | 163 | 1 | 165 | 2.00e-18 | 82.4 |
| MS.gene99631.t1 | MTR_2g090580 | 30.899 | 178 | 106 | 4 | 1 | 161 | 1 | 178 | 2.41e-18 | 79.0 |
| MS.gene99631.t1 | MTR_8g463180 | 29.851 | 134 | 91 | 2 | 4 | 134 | 1 | 134 | 2.54e-18 | 80.9 |
| MS.gene99631.t1 | MTR_4g111640 | 31.210 | 157 | 97 | 4 | 25 | 177 | 32 | 181 | 7.01e-18 | 77.4 |
| MS.gene99631.t1 | MTR_4g077787 | 35.338 | 133 | 76 | 4 | 25 | 149 | 32 | 162 | 1.10e-17 | 77.0 |
| MS.gene99631.t1 | MTR_1g072180 | 38.835 | 103 | 41 | 1 | 20 | 122 | 19 | 99 | 1.37e-17 | 74.3 |
| MS.gene99631.t1 | MTR_4g081100 | 33.628 | 113 | 73 | 1 | 13 | 123 | 20 | 132 | 2.42e-17 | 77.0 |
| MS.gene99631.t1 | MTR_6g022170 | 38.235 | 102 | 58 | 2 | 25 | 123 | 34 | 133 | 4.61e-17 | 74.7 |
| MS.gene99631.t1 | MTR_2g043790 | 35.922 | 103 | 61 | 2 | 19 | 121 | 1 | 98 | 1.25e-16 | 72.0 |
| MS.gene99631.t1 | MTR_7g086090 | 38.636 | 132 | 71 | 4 | 7 | 138 | 14 | 135 | 1.32e-16 | 75.1 |
| MS.gene99631.t1 | MTR_4g112310 | 32.117 | 137 | 86 | 3 | 41 | 177 | 2 | 131 | 5.70e-16 | 71.2 |
| MS.gene99631.t1 | MTR_6g080660 | 32.653 | 98 | 63 | 2 | 25 | 122 | 28 | 122 | 1.15e-15 | 72.0 |
| MS.gene99631.t1 | MTR_8g099220 | 30.709 | 127 | 76 | 3 | 6 | 122 | 3 | 127 | 1.44e-15 | 70.1 |
| MS.gene99631.t1 | MTR_4g114830 | 34.615 | 130 | 77 | 4 | 15 | 139 | 18 | 144 | 1.59e-15 | 70.9 |
| MS.gene99631.t1 | MTR_6g013420 | 30.303 | 132 | 83 | 4 | 13 | 142 | 26 | 150 | 4.45e-15 | 70.1 |
| MS.gene99631.t1 | MTR_0334s0010 | 29.310 | 116 | 80 | 2 | 8 | 122 | 11 | 125 | 9.66e-15 | 69.3 |
| MS.gene99631.t1 | MTR_4g124280 | 30.625 | 160 | 94 | 4 | 21 | 164 | 21 | 179 | 1.16e-14 | 70.1 |
| MS.gene99631.t1 | MTR_2g090575 | 29.801 | 151 | 105 | 1 | 8 | 157 | 8 | 158 | 4.35e-14 | 69.7 |
| MS.gene99631.t1 | MTR_8g094990 | 28.947 | 114 | 80 | 1 | 8 | 120 | 8 | 121 | 4.39e-14 | 68.9 |
| MS.gene99631.t1 | MTR_5g088180 | 34.167 | 120 | 73 | 4 | 28 | 145 | 33 | 148 | 6.91e-14 | 66.6 |
| MS.gene99631.t1 | MTR_2g025580 | 31.624 | 117 | 76 | 3 | 8 | 122 | 9 | 123 | 8.03e-14 | 66.2 |
| MS.gene99631.t1 | MTR_6g013200 | 30.769 | 117 | 76 | 3 | 8 | 122 | 18 | 131 | 8.69e-14 | 66.2 |
| MS.gene99631.t1 | MTR_5g088170 | 35.043 | 117 | 70 | 4 | 28 | 142 | 20 | 132 | 1.01e-13 | 65.9 |
| MS.gene99631.t1 | MTR_6g013170 | 30.233 | 129 | 82 | 5 | 24 | 150 | 30 | 152 | 2.63e-13 | 65.1 |
| MS.gene99631.t1 | MTR_4g067200 | 28.369 | 141 | 90 | 4 | 1 | 134 | 1 | 137 | 7.87e-13 | 63.9 |
| MS.gene99631.t1 | MTR_4g066110 | 28.369 | 141 | 90 | 4 | 1 | 134 | 1 | 137 | 7.87e-13 | 63.9 |
| MS.gene99631.t1 | MTR_8g095013 | 29.940 | 167 | 103 | 3 | 8 | 161 | 8 | 173 | 3.14e-12 | 63.9 |
| MS.gene99631.t1 | MTR_2g083250 | 26.400 | 125 | 88 | 2 | 3 | 123 | 4 | 128 | 4.48e-12 | 63.2 |
| MS.gene99631.t1 | MTR_3g117110 | 27.333 | 150 | 92 | 6 | 4 | 146 | 3 | 142 | 1.99e-11 | 60.1 |
| MS.gene99631.t1 | MTR_8g095020 | 30.464 | 151 | 84 | 5 | 24 | 157 | 24 | 170 | 3.21e-11 | 61.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene99631.t1 | AT5G26330 | 57.576 | 165 | 69 | 1 | 10 | 174 | 9 | 172 | 2.07e-64 | 196 |
| MS.gene99631.t1 | AT2G26720 | 38.298 | 141 | 82 | 2 | 1 | 137 | 1 | 140 | 2.09e-34 | 120 |
| MS.gene99631.t1 | AT2G31050 | 42.636 | 129 | 72 | 2 | 10 | 137 | 13 | 140 | 1.04e-33 | 119 |
| MS.gene99631.t1 | AT2G32300 | 34.940 | 166 | 93 | 5 | 1 | 165 | 1 | 152 | 1.12e-31 | 115 |
| MS.gene99631.t1 | AT2G32300 | 34.940 | 166 | 93 | 5 | 1 | 165 | 31 | 182 | 3.54e-31 | 114 |
| MS.gene99631.t1 | AT3G27200 | 32.432 | 111 | 71 | 3 | 14 | 123 | 15 | 122 | 3.23e-23 | 91.3 |
| MS.gene99631.t1 | AT3G17675 | 41.667 | 96 | 54 | 1 | 27 | 122 | 34 | 127 | 8.57e-23 | 89.0 |
| MS.gene99631.t1 | AT5G07475 | 34.839 | 155 | 87 | 5 | 14 | 168 | 19 | 159 | 9.55e-23 | 90.5 |
| MS.gene99631.t1 | AT4G31840 | 32.692 | 156 | 93 | 4 | 28 | 180 | 31 | 177 | 3.44e-22 | 88.6 |
| MS.gene99631.t1 | AT4G28365 | 30.508 | 177 | 107 | 4 | 1 | 161 | 1 | 177 | 8.04e-22 | 88.2 |
| MS.gene99631.t1 | AT3G60270 | 37.864 | 103 | 61 | 2 | 21 | 123 | 22 | 121 | 2.30e-20 | 84.0 |
| MS.gene99631.t1 | AT3G53330 | 35.238 | 105 | 67 | 1 | 19 | 122 | 182 | 286 | 3.24e-20 | 86.3 |
| MS.gene99631.t1 | AT3G53330 | 39.080 | 87 | 47 | 3 | 7 | 92 | 8 | 89 | 1.88e-13 | 67.4 |
| MS.gene99631.t1 | AT1G45063 | 40.000 | 100 | 59 | 1 | 24 | 122 | 148 | 247 | 1.19e-19 | 84.0 |
| MS.gene99631.t1 | AT1G45063 | 32.031 | 128 | 81 | 3 | 1 | 123 | 1 | 127 | 1.14e-17 | 78.6 |
| MS.gene99631.t1 | AT2G02850 | 31.858 | 113 | 72 | 2 | 8 | 120 | 19 | 126 | 1.50e-19 | 80.5 |
| MS.gene99631.t1 | AT5G20230 | 34.646 | 127 | 78 | 2 | 25 | 150 | 25 | 147 | 1.57e-19 | 82.0 |
| MS.gene99631.t1 | AT1G45063 | 40.000 | 100 | 59 | 1 | 24 | 122 | 148 | 247 | 2.02e-19 | 84.7 |
| MS.gene99631.t1 | AT1G45063 | 32.031 | 128 | 81 | 3 | 1 | 123 | 1 | 127 | 1.82e-17 | 79.3 |
| MS.gene99631.t1 | AT3G20570 | 29.091 | 165 | 106 | 3 | 9 | 163 | 15 | 178 | 2.98e-19 | 81.6 |
| MS.gene99631.t1 | AT5G53870 | 30.114 | 176 | 109 | 4 | 1 | 165 | 1 | 173 | 3.14e-19 | 84.0 |
| MS.gene99631.t1 | AT5G53870 | 30.114 | 176 | 109 | 4 | 1 | 165 | 1 | 173 | 5.21e-19 | 83.6 |
| MS.gene99631.t1 | AT3G60280 | 39.806 | 103 | 59 | 2 | 21 | 123 | 20 | 119 | 6.86e-19 | 80.9 |
| MS.gene99631.t1 | AT2G25060 | 33.588 | 131 | 81 | 3 | 10 | 134 | 14 | 144 | 9.79e-19 | 79.7 |
| MS.gene99631.t1 | AT1G72230 | 31.429 | 140 | 91 | 2 | 40 | 175 | 36 | 174 | 1.00e-18 | 79.7 |
| MS.gene99631.t1 | AT1G64640 | 29.341 | 167 | 92 | 4 | 4 | 161 | 6 | 155 | 3.05e-18 | 78.6 |
| MS.gene99631.t1 | AT4G30590 | 31.538 | 130 | 81 | 4 | 1 | 123 | 1 | 129 | 2.07e-17 | 76.3 |
| MS.gene99631.t1 | AT5G57920 | 32.632 | 95 | 60 | 1 | 43 | 133 | 44 | 138 | 5.14e-17 | 75.1 |
| MS.gene99631.t1 | AT5G57920 | 27.007 | 137 | 94 | 2 | 3 | 133 | 1 | 137 | 1.05e-16 | 74.3 |
| MS.gene99631.t1 | AT4G27520 | 31.034 | 145 | 93 | 3 | 9 | 146 | 15 | 159 | 1.07e-16 | 77.0 |
| MS.gene99631.t1 | AT5G15350 | 33.835 | 133 | 80 | 4 | 7 | 137 | 10 | 136 | 1.40e-16 | 73.9 |
| MS.gene99631.t1 | AT5G57920 | 31.250 | 112 | 71 | 2 | 28 | 133 | 59 | 170 | 2.11e-16 | 74.3 |
| MS.gene99631.t1 | AT1G79800 | 30.882 | 136 | 85 | 3 | 9 | 137 | 18 | 151 | 4.62e-16 | 72.8 |
| MS.gene99631.t1 | AT2G44790 | 37.634 | 93 | 53 | 3 | 33 | 123 | 36 | 125 | 6.22e-16 | 72.8 |
| MS.gene99631.t1 | AT1G22480 | 31.746 | 189 | 105 | 7 | 1 | 183 | 1 | 171 | 9.77e-16 | 71.6 |
| MS.gene99631.t1 | AT4G32490 | 28.402 | 169 | 98 | 4 | 8 | 158 | 12 | 175 | 4.46e-15 | 70.9 |
| MS.gene99631.t1 | AT5G25090 | 33.333 | 81 | 54 | 0 | 43 | 123 | 47 | 127 | 4.84e-15 | 70.1 |
| MS.gene99631.t1 | AT3G18590 | 34.286 | 105 | 59 | 5 | 25 | 123 | 27 | 127 | 4.97e-15 | 70.1 |
| MS.gene99631.t1 | AT2G23990 | 36.250 | 80 | 50 | 1 | 44 | 122 | 49 | 128 | 1.11e-14 | 69.3 |
| MS.gene99631.t1 | AT5G14345 | 37.255 | 102 | 58 | 3 | 41 | 141 | 35 | 131 | 5.35e-14 | 66.2 |
| MS.gene99631.t1 | AT1G48940 | 31.538 | 130 | 77 | 6 | 5 | 126 | 4 | 129 | 1.01e-13 | 66.2 |
| MS.gene99631.t1 | AT4G01380 | 28.205 | 156 | 79 | 6 | 30 | 180 | 77 | 204 | 2.06e-13 | 66.2 |
| MS.gene99631.t1 | AT4G12880 | 33.010 | 103 | 64 | 3 | 22 | 122 | 26 | 125 | 6.74e-13 | 63.2 |
| MS.gene99631.t1 | AT2G27035 | 25.161 | 155 | 104 | 5 | 2 | 155 | 8 | 151 | 1.26e-11 | 60.5 |
| MS.gene99631.t1 | AT1G17800 | 29.167 | 120 | 78 | 4 | 8 | 123 | 21 | 137 | 2.41e-11 | 59.3 |
Find 31 sgRNAs with CRISPR-Local
Find 81 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACCATGATTTGTTATCTTTA+TGG | 0.100250 | 1.4:-80668780 | None:intergenic |
| GTCACCAAGTTGGAAGTTCT+TGG | 0.347606 | 1.4:-80667872 | None:intergenic |
| ATCATCTTCACCTTCAGCAT+TGG | 0.417122 | 1.4:+80668901 | MS.gene99631:CDS |
| AGAGTCTATAGTCATGGCTT+TGG | 0.467395 | 1.4:+80667720 | None:intergenic |
| CCAATTGCAACATTCACTAC+TGG | 0.482441 | 1.4:+80668749 | MS.gene99631:CDS |
| TTCAGTGGTGTAGCATTACT+AGG | 0.484549 | 1.4:-80668974 | None:intergenic |
| TTCATATGCAGCTGTATACA+AGG | 0.487437 | 1.4:+80667789 | MS.gene99631:CDS |
| CCAGTAGTGAATGTTGCAAT+TGG | 0.487702 | 1.4:-80668749 | None:intergenic |
| AGGTTGGTGATTCTGCTGGC+TGG | 0.497516 | 1.4:+80667809 | MS.gene99631:CDS |
| TACAAGGTTGGTGATTCTGC+TGG | 0.511253 | 1.4:+80667805 | MS.gene99631:CDS |
| ACCATAAAGATAACAAATCA+TGG | 0.515142 | 1.4:+80668779 | MS.gene99631:CDS |
| TGTCCTGCTTGACAGTGTCC+AGG | 0.520727 | 1.4:-80668827 | None:intergenic |
| GGCTTTGGTAGAAAGAGCTA+TGG | 0.525531 | 1.4:+80667735 | MS.gene99631:CDS |
| AGGAGAAGGTGCAGGTACAT+TGG | 0.525671 | 1.4:-80668954 | None:intergenic |
| GCTGGAACTGTAGCTTCAGC+AGG | 0.528803 | 1.4:-80668929 | None:intergenic |
| GCAGGAGAAGCCAATGCTGA+AGG | 0.557674 | 1.4:-80668911 | None:intergenic |
| GTACCTGGACACTGTCAAGC+AGG | 0.560336 | 1.4:+80668824 | MS.gene99631:CDS |
| TATGCAGCTGTATACAAGGT+TGG | 0.562762 | 1.4:+80667793 | MS.gene99631:CDS |
| TACTAATAGTGTCACCAAGT+TGG | 0.563363 | 1.4:-80667882 | None:intergenic |
| GGTGCAGGTACATTGGAAGC+TGG | 0.574590 | 1.4:-80668947 | None:intergenic |
| GCTGCCAAGAACTTCCAACT+TGG | 0.575582 | 1.4:+80667868 | MS.gene99631:CDS |
| AATGCTGAAGGTGAAGATGA+TGG | 0.589576 | 1.4:-80668899 | None:intergenic |
| TTTAAAGCAATACATTTCAG+TGG | 0.593570 | 1.4:-80668989 | None:intergenic |
| GGTGTAGCATTACTAGGAGA+AGG | 0.597398 | 1.4:-80668968 | None:intergenic |
| ATTAACAGCAAGAAATTGCA+TGG | 0.600755 | 1.4:-80669032 | None:intergenic |
| ACACTGTCAAGCAGGACAGA+AGG | 0.613934 | 1.4:+80668832 | MS.gene99631:CDS |
| TCTGCTGGCTGGACTACACT+TGG | 0.619967 | 1.4:+80667820 | MS.gene99631:CDS |
| GAAGGTGAAGATGATGGAGC+AGG | 0.628782 | 1.4:-80668893 | None:intergenic |
| TGCATTGCATGACTTGTACA+TGG | 0.629046 | 1.4:-80668720 | None:intergenic |
| GTGTCACTCGCATCACATTG+TGG | 0.630592 | 1.4:-80668694 | None:intergenic |
| GCATTACTAGGAGAAGGTGC+AGG | 0.651848 | 1.4:-80668962 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTAACTAAAAGATTTTTTAT+CGG | - | chr1.4:80668375-80668394 | None:intergenic | 10.0% |
| !!! | TTGTAAAATAATGTTAATTT+TGG | - | chr1.4:80668027-80668046 | None:intergenic | 10.0% |
| !!! | AAATTAAGCATTTTTTTCAA+GGG | + | chr1.4:80668231-80668250 | MS.gene99631:intron | 15.0% |
| !!! | AACATTATTTTACAATTCTA+TGG | + | chr1.4:80668032-80668051 | MS.gene99631:intron | 15.0% |
| !!! | TAAATTAAGCATTTTTTTCA+AGG | + | chr1.4:80668230-80668249 | MS.gene99631:intron | 15.0% |
| !! | AACTCAAAAAAAAAATTGAG+AGG | + | chr1.4:80668599-80668618 | MS.gene99631:intron | 20.0% |
| !! | TATTTCTTCAACAAATTAGA+CGG | - | chr1.4:80668312-80668331 | None:intergenic | 20.0% |
| !!! | TAACATTGACTACAAAAAAT+GGG | + | chr1.4:80667843-80667862 | MS.gene99631:CDS | 20.0% |
| ! | ACCATAAAGATAACAAATCA+TGG | + | chr1.4:80668779-80668798 | MS.gene99631:CDS | 25.0% |
| ! | ATGAAAACAAAAAGTTGTGT+AGG | - | chr1.4:80668193-80668212 | None:intergenic | 25.0% |
| ! | GAACAAATTAAACATGCAAT+TGG | - | chr1.4:80667918-80667937 | None:intergenic | 25.0% |
| !! | ATCATGAAATTGATTTATCC+GGG | - | chr1.4:80668416-80668435 | None:intergenic | 25.0% |
| !! | CATCATGAAATTGATTTATC+CGG | - | chr1.4:80668417-80668436 | None:intergenic | 25.0% |
| !! | GTAACATTGACTACAAAAAA+TGG | + | chr1.4:80667842-80667861 | MS.gene99631:CDS | 25.0% |
| !! | TGAGTTTTTCTATACCAAAT+TGG | - | chr1.4:80668078-80668097 | None:intergenic | 25.0% |
| !! | TGTTGAAGAAATAGATCTTT+TGG | + | chr1.4:80668319-80668338 | MS.gene99631:intron | 25.0% |
| !! | TTTAAAGCAATACATTTCAG+TGG | - | chr1.4:80668992-80669011 | None:intergenic | 25.0% |
| !! | TTTTCTAACATTGTCCAATT+TGG | + | chr1.4:80668061-80668080 | MS.gene99631:intron | 25.0% |
| !!! | ACCATGATTTGTTATCTTTA+TGG | - | chr1.4:80668783-80668802 | None:intergenic | 25.0% |
| !!! | TATGGTTTTGTCTATAATGA+TGG | + | chr1.4:80667753-80667772 | MS.gene99631:CDS | 25.0% |
| !!! | TATTGCTTTAAAAATGATGC+TGG | + | chr1.4:80669000-80669019 | MS.gene99631:CDS | 25.0% |
| ATTAACAGCAAGAAATTGCA+TGG | - | chr1.4:80669035-80669054 | None:intergenic | 30.0% | |
| ATTGGACAATGTTAGAAAAG+AGG | - | chr1.4:80668060-80668079 | None:intergenic | 30.0% | |
| TTAAACATGCAATTGGAGAA+AGG | - | chr1.4:80667911-80667930 | None:intergenic | 30.0% | |
| !! | TGTAGGTCTTAATTAGTCTT+GGG | - | chr1.4:80668176-80668195 | None:intergenic | 30.0% |
| !!! | AAAAATGATGCTGGCTTTTT+GGG | + | chr1.4:80669009-80669028 | MS.gene99631:CDS | 30.0% |
| !!! | CACATTTTCTGAAGTTTGAA+AGG | + | chr1.4:80667980-80667999 | MS.gene99631:intron | 30.0% |
| !!! | TAAAAATGATGCTGGCTTTT+TGG | + | chr1.4:80669008-80669027 | MS.gene99631:CDS | 30.0% |
| !!! | TTTTTCAAGGGAAACGTTAA+CGG | + | chr1.4:80668243-80668262 | MS.gene99631:intron | 30.0% |
| AAAGGTAATGCAAGTGAAAG+TGG | - | chr1.4:80668464-80668483 | None:intergenic | 35.0% | |
| AAGTTTGAAAGGTCCTTTCA+AGG | + | chr1.4:80667991-80668010 | MS.gene99631:intron | 35.0% | |
| ACAACATGCAATGACTTTGT+TGG | + | chr1.4:80668518-80668537 | MS.gene99631:intron | 35.0% | |
| AGTTTGAAAGGTCCTTTCAA+GGG | + | chr1.4:80667992-80668011 | MS.gene99631:intron | 35.0% | |
| CAACATGCAATGACTTTGTT+GGG | + | chr1.4:80668519-80668538 | MS.gene99631:intron | 35.0% | |
| CACTATTGCACTATCTTTCA+TGG | + | chr1.4:80668135-80668154 | MS.gene99631:intron | 35.0% | |
| CATGAAAGATAGTGCAATAG+TGG | - | chr1.4:80668137-80668156 | None:intergenic | 35.0% | |
| CTTGAAGCAAAAACATGGAA+AGG | - | chr1.4:80668108-80668127 | None:intergenic | 35.0% | |
| GATCTGAATTCGAATTATCC+CGG | + | chr1.4:80668395-80668414 | MS.gene99631:intron | 35.0% | |
| TACTAATAGTGTCACCAAGT+TGG | - | chr1.4:80667885-80667904 | None:intergenic | 35.0% | |
| TTCATATGCAGCTGTATACA+AGG | + | chr1.4:80667789-80667808 | MS.gene99631:CDS | 35.0% | |
| ! | GTGTAGGTCTTAATTAGTCT+TGG | - | chr1.4:80668177-80668196 | None:intergenic | 35.0% |
| ! | TGCATTACCTTTTTGTTCTG+TGG | + | chr1.4:80668472-80668491 | MS.gene99631:intron | 35.0% |
| !! | CTTTCCATGTTTTTGCTTCA+AGG | + | chr1.4:80668106-80668125 | MS.gene99631:intron | 35.0% |
| !! | TTTTCAAGGGAAACGTTAAC+GGG | + | chr1.4:80668244-80668263 | MS.gene99631:intron | 35.0% |
| AACCATGCTCTCGATGATTT+AGG | - | chr1.4:80668549-80668568 | None:intergenic | 40.0% | |
| AATGCTGAAGGTGAAGATGA+TGG | - | chr1.4:80668902-80668921 | None:intergenic | 40.0% | |
| ATCATCTTCACCTTCAGCAT+TGG | + | chr1.4:80668901-80668920 | MS.gene99631:CDS | 40.0% | |
| CCAATTGCAACATTCACTAC+TGG | + | chr1.4:80668749-80668768 | MS.gene99631:CDS | 40.0% | |
| CCAGTAGTGAATGTTGCAAT+TGG | - | chr1.4:80668752-80668771 | None:intergenic | 40.0% | |
| GAAGCAAAAACATGGAAAGG+AGG | - | chr1.4:80668105-80668124 | None:intergenic | 40.0% | |
| GTAATGCAAGTGAAAGTGGT+GGG | - | chr1.4:80668460-80668479 | None:intergenic | 40.0% | |
| TATGCAGCTGTATACAAGGT+TGG | + | chr1.4:80667793-80667812 | MS.gene99631:CDS | 40.0% | |
| TTCAGTGGTGTAGCATTACT+AGG | - | chr1.4:80668977-80668996 | None:intergenic | 40.0% | |
| TTGCACTATCTTTCATGGCA+TGG | + | chr1.4:80668140-80668159 | MS.gene99631:intron | 40.0% | |
| ! | CAAAGCACCACAGAACAAAA+AGG | - | chr1.4:80668482-80668501 | None:intergenic | 40.0% |
| ! | GGTCATCACTTCTTCTTTTG+TGG | + | chr1.4:80668800-80668819 | MS.gene99631:CDS | 40.0% |
| ! | GTAGCCTTGAAGCAAAAACA+TGG | - | chr1.4:80668113-80668132 | None:intergenic | 40.0% |
| ! | TGCATTGCATGACTTGTACA+TGG | - | chr1.4:80668723-80668742 | None:intergenic | 40.0% |
| ! | TTCTTCTTTTGTGGTGTACC+TGG | + | chr1.4:80668809-80668828 | MS.gene99631:CDS | 40.0% |
| CATGCAATGACTTTGTTGGG+TGG | + | chr1.4:80668522-80668541 | MS.gene99631:intron | 45.0% | |
| GGTAATGCAAGTGAAAGTGG+TGG | - | chr1.4:80668461-80668480 | None:intergenic | 45.0% | |
| GGTGTAGCATTACTAGGAGA+AGG | - | chr1.4:80668971-80668990 | None:intergenic | 45.0% | |
| GTCCTAAATCATCGAGAGCA+TGG | + | chr1.4:80668544-80668563 | MS.gene99631:intron | 45.0% | |
| TGGTTGTCTTCTCCCTTGAA+AGG | - | chr1.4:80668007-80668026 | None:intergenic | 45.0% | |
| ! | GGCTTTGGTAGAAAGAGCTA+TGG | + | chr1.4:80667735-80667754 | MS.gene99631:CDS | 45.0% |
| ! | GTCACCAAGTTGGAAGTTCT+TGG | - | chr1.4:80667875-80667894 | None:intergenic | 45.0% |
| !! | TACAAGGTTGGTGATTCTGC+TGG | + | chr1.4:80667805-80667824 | MS.gene99631:CDS | 45.0% |
| ACACTGTCAAGCAGGACAGA+AGG | + | chr1.4:80668832-80668851 | MS.gene99631:CDS | 50.0% | |
| GCTGCCAAGAACTTCCAACT+TGG | + | chr1.4:80667868-80667887 | MS.gene99631:CDS | 50.0% | |
| TGGTCTCGAGCTCGATTGAA+AGG | + | chr1.4:80668339-80668358 | MS.gene99631:intron | 50.0% | |
| ! | AGGAGAAGGTGCAGGTACAT+TGG | - | chr1.4:80668957-80668976 | None:intergenic | 50.0% |
| ! | GCATTACTAGGAGAAGGTGC+AGG | - | chr1.4:80668965-80668984 | None:intergenic | 50.0% |
| ! | GTGTCACTCGCATCACATTG+TGG | - | chr1.4:80668697-80668716 | None:intergenic | 50.0% |
| !! | GAAGGTGAAGATGATGGAGC+AGG | - | chr1.4:80668896-80668915 | None:intergenic | 50.0% |
| GCAGGAGAAGCCAATGCTGA+AGG | - | chr1.4:80668914-80668933 | None:intergenic | 55.0% | |
| GCTGGAACTGTAGCTTCAGC+AGG | - | chr1.4:80668932-80668951 | None:intergenic | 55.0% | |
| GGTGCAGGTACATTGGAAGC+TGG | - | chr1.4:80668950-80668969 | None:intergenic | 55.0% | |
| GTACCTGGACACTGTCAAGC+AGG | + | chr1.4:80668824-80668843 | MS.gene99631:CDS | 55.0% | |
| TGTCCTGCTTGACAGTGTCC+AGG | - | chr1.4:80668830-80668849 | None:intergenic | 55.0% | |
| ! | TCTGCTGGCTGGACTACACT+TGG | + | chr1.4:80667820-80667839 | MS.gene99631:CDS | 55.0% |
| !! | AGGTTGGTGATTCTGCTGGC+TGG | + | chr1.4:80667809-80667828 | MS.gene99631:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 80667733 | 80669060 | 80667733 | ID=MS.gene99631 |
| chr1.4 | mRNA | 80667733 | 80669060 | 80667733 | ID=MS.gene99631.t1;Parent=MS.gene99631 |
| chr1.4 | exon | 80667733 | 80667901 | 80667733 | ID=MS.gene99631.t1.exon1;Parent=MS.gene99631.t1 |
| chr1.4 | CDS | 80667733 | 80667901 | 80667733 | ID=cds.MS.gene99631.t1;Parent=MS.gene99631.t1 |
| chr1.4 | exon | 80668672 | 80669060 | 80668672 | ID=MS.gene99631.t1.exon2;Parent=MS.gene99631.t1 |
| chr1.4 | CDS | 80668672 | 80669060 | 80668672 | ID=cds.MS.gene99631.t1;Parent=MS.gene99631.t1 |
| Gene Sequence |
| Protein sequence |
