AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene99722

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99722.t1 MTR_5g074130 88.689 557 63 0 1 557 121 677 0.0 1021
MS.gene99722.t1 MTR_5g093530 81.972 355 41 1 204 558 244 575 0.0 598
MS.gene99722.t1 MTR_5g093530 83.088 136 23 0 1 136 121 256 1.55e-65 224
MS.gene99722.t1 MTR_5g073920 49.821 560 257 5 1 554 117 658 0.0 536
MS.gene99722.t1 MTR_3g464010 46.341 574 268 9 1 557 120 670 1.18e-161 476
MS.gene99722.t1 MTR_5g073460 44.387 579 288 12 1 553 120 690 1.31e-137 415
MS.gene99722.t1 MTR_5g073450 47.045 440 211 9 129 553 199 631 4.18e-115 355
MS.gene99722.t1 MTR_3g031130 46.396 444 206 11 128 553 188 617 4.31e-107 334
MS.gene99722.t1 MTR_5g073620 42.259 478 221 9 1 436 120 584 8.66e-103 333
MS.gene99722.t1 MTR_5g073620 37.870 338 181 7 223 554 753 1067 4.67e-56 204
MS.gene99722.t1 MTR_5g073480 45.823 419 201 10 1 397 120 534 3.91e-101 318
MS.gene99722.t1 MTR_5g073900 39.948 383 192 9 149 529 1 347 1.39e-72 236
MS.gene99722.t1 MTR_5g073960 44.884 303 151 5 7 305 126 416 1.73e-69 230
MS.gene99722.t1 MTR_5g073780 44.915 236 116 3 319 554 1 222 2.38e-60 199
MS.gene99722.t1 MTR_1g090620 29.677 465 288 10 113 553 190 639 2.61e-60 211
MS.gene99722.t1 MTR_7g026630 30.603 464 285 10 113 553 199 648 1.19e-57 204
MS.gene99722.t1 MTR_1g057560 31.323 431 276 8 134 553 202 623 1.34e-56 201
MS.gene99722.t1 MTR_5g073770 41.356 295 159 8 25 305 85 379 1.98e-54 189
MS.gene99722.t1 MTR_8g021330 28.125 448 292 10 131 553 161 603 3.96e-53 191
MS.gene99722.t1 MTR_4g103540 28.894 443 293 9 126 553 243 678 9.64e-50 182
MS.gene99722.t1 MTR_5g073650 36.657 341 147 7 2 315 20 318 7.45e-49 172
MS.gene99722.t1 MTR_6g033310 28.635 447 278 10 137 553 288 723 6.19e-47 175
MS.gene99722.t1 MTR_5g073810 36.765 340 146 7 3 315 123 420 8.63e-47 169
MS.gene99722.t1 MTR_7g013770 26.949 449 294 10 130 553 225 664 4.13e-44 166
MS.gene99722.t1 MTR_1g117090 25.957 470 280 12 142 554 209 667 1.34e-38 150
MS.gene99722.t1 MTR_5g073820 31.132 424 159 12 8 400 108 429 1.90e-36 142
MS.gene99722.t1 MTR_3g025900 25.799 438 298 9 131 553 181 606 1.91e-34 138
MS.gene99722.t1 MTR_1g067460 24.289 457 295 13 131 555 164 601 2.75e-34 137
MS.gene99722.t1 MTR_1g067480 23.725 451 300 10 131 553 166 600 4.22e-34 137
MS.gene99722.t1 MTR_5g073610 41.509 212 101 5 2 207 122 316 1.38e-33 130
MS.gene99722.t1 MTR_2g096230 22.422 446 310 10 126 553 152 579 4.22e-32 130
MS.gene99722.t1 MTR_4g062330 24.263 441 286 11 142 553 181 602 1.16e-31 130
MS.gene99722.t1 MTR_5g073750 45.062 162 85 2 2 159 121 282 6.07e-30 119
MS.gene99722.t1 MTR_5g074190 30.928 291 136 6 267 556 184 410 7.15e-30 123
MS.gene99722.t1 MTR_5g074190 63.158 57 21 0 1 57 114 170 2.50e-11 66.6
MS.gene99722.t1 MTR_5g073470 36.893 206 93 5 1 201 96 269 2.30e-28 114
MS.gene99722.t1 MTR_5g073490 36.970 165 85 3 390 552 4 151 1.29e-23 97.8
MS.gene99722.t1 MTR_4g019260 73.333 60 16 0 212 271 10 69 2.35e-23 94.0
MS.gene99722.t1 MTR_5g073660 42.763 152 60 4 8 132 108 259 8.27e-23 99.4
MS.gene99722.t1 MTR_1g009620 21.281 484 345 11 87 553 187 651 1.25e-20 96.3
MS.gene99722.t1 MTR_5g074450 48.485 132 45 5 1 109 120 251 1.34e-20 95.9
MS.gene99722.t1 MTR_5g073880 41.176 153 64 5 1 149 121 251 1.26e-17 83.6
MS.gene99722.t1 MTR_4g008260 22.715 383 262 10 189 553 273 639 1.46e-17 86.7
MS.gene99722.t1 MTR_5g073840 50.549 91 40 2 1 86 120 210 2.92e-16 79.3
MS.gene99722.t1 MTR_5g074200 51.351 74 36 0 1 74 117 190 6.24e-12 67.4
MS.gene99722.t1 MTR_0229s0040 24.432 176 131 1 380 553 31 206 8.10e-12 65.9
MS.gene99722.t1 MTR_5g074150 62.000 50 19 0 1 50 131 180 6.03e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99722.t1 AT5G58430 44.690 452 226 11 115 553 176 616 8.34e-105 328
MS.gene99722.t1 AT1G07000 36.054 441 254 12 129 558 176 599 7.37e-69 233
MS.gene99722.t1 AT5G50380 30.337 445 288 10 126 553 230 669 5.16e-57 203
MS.gene99722.t1 AT5G52340 30.415 434 274 11 134 553 202 621 1.20e-55 198
MS.gene99722.t1 AT5G03540 29.655 435 283 8 134 553 87 513 7.83e-54 191
MS.gene99722.t1 AT5G03540 29.655 435 283 8 134 553 202 628 5.07e-53 191
MS.gene99722.t1 AT5G03540 29.655 435 283 8 134 553 228 654 7.44e-53 191
MS.gene99722.t1 AT1G72470 30.649 447 281 11 131 553 178 619 2.35e-52 189
MS.gene99722.t1 AT1G54090 28.859 447 287 11 131 553 170 609 9.00e-52 187
MS.gene99722.t1 AT5G13150 29.556 450 269 12 136 553 209 642 1.82e-51 187
MS.gene99722.t1 AT3G14090 28.146 437 288 11 131 546 173 604 4.75e-50 182
MS.gene99722.t1 AT5G13990 26.908 498 324 14 70 553 208 679 3.61e-45 170
MS.gene99722.t1 AT5G52350 28.372 430 288 10 134 553 156 575 2.07e-43 163
MS.gene99722.t1 AT2G39380 25.322 466 316 9 110 553 153 608 2.11e-40 155
MS.gene99722.t1 AT3G29400 25.431 464 296 10 128 553 194 645 2.20e-38 150
MS.gene99722.t1 AT3G55150 27.107 439 284 11 138 553 183 608 5.56e-36 142
MS.gene99722.t1 AT5G61010 25.169 445 285 12 132 553 205 624 5.81e-34 137
MS.gene99722.t1 AT5G61010 25.169 445 285 12 132 553 205 624 5.81e-34 137
MS.gene99722.t1 AT1G07725 24.083 436 293 9 137 554 175 590 2.54e-33 134
MS.gene99722.t1 AT2G28640 25.397 441 288 12 137 554 155 577 1.06e-32 132
MS.gene99722.t1 AT3G09520 22.907 454 310 11 129 553 162 604 1.59e-31 129
MS.gene99722.t1 AT3G09530 23.894 452 309 12 129 553 157 600 1.96e-30 126
MS.gene99722.t1 AT5G59730 24.053 449 292 14 137 553 165 596 1.14e-28 120
MS.gene99722.t1 AT5G59730 24.053 449 292 14 137 553 165 596 1.64e-28 120
MS.gene99722.t1 AT4G31540 22.321 448 311 12 129 554 223 655 4.71e-22 100
MS.gene99722.t1 AT2G28650 24.427 393 270 10 146 527 156 532 2.99e-20 94.7
MS.gene99722.t1 AT1G51640 24.425 348 236 8 200 526 253 594 5.91e-15 78.6

Find 93 sgRNAs with CRISPR-Local

Find 109 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGCTTCTCCTAATCTGTTT+TGG 0.232404 5.2:+62120504 None:intergenic
GAACCAATCGTTACCAAATA+TGG 0.238501 5.2:+62120127 None:intergenic
TTTAATTAAAAGGTGGGATT+TGG 0.239403 5.2:-62120153 MS.gene99722:CDS
TGGGATTTGGAAACCATATT+TGG 0.243486 5.2:-62120140 MS.gene99722:CDS
AAGTTGCAGTTATTCATTAT+TGG 0.254047 5.2:-62121298 MS.gene99722:CDS
AATACCTTGCTGCCAGTATA+TGG 0.291063 5.2:-62119858 MS.gene99722:CDS
AGCAAGGTCTCTGTACTCTT+TGG 0.301069 5.2:+62120220 None:intergenic
TGTCTAATCAATAGATTATT+CGG 0.316039 5.2:-62120878 MS.gene99722:CDS
ACATAGAAGATTTAATTAAA+AGG 0.323041 5.2:-62120163 MS.gene99722:CDS
CCACTTGGGTGCTGATTCTT+CGG 0.339621 5.2:-62120549 MS.gene99722:CDS
ACAACAGATGAAATGGATAA+TGG 0.343765 5.2:-62120273 MS.gene99722:CDS
GATAATTTAGTTTCAAGAAT+AGG 0.346022 5.2:+62120474 None:intergenic
CTCTGATGCCACATCATCAT+TGG 0.349680 5.2:+62119998 None:intergenic
GACTCTGATTTGATGTTGCC+TGG 0.369550 5.2:-62121040 MS.gene99722:CDS
ACTACTCCATTATTCACCTT+TGG 0.373276 5.2:+62120314 None:intergenic
TGAATTCCAAGATGAAAATT+CGG 0.373996 5.2:-62121206 MS.gene99722:CDS
TCTAATCAGTATATTAGATA+TGG 0.374693 5.2:-62119795 MS.gene99722:CDS
GTTGGACAACAGTGACTATC+TGG 0.377423 5.2:-62120024 MS.gene99722:CDS
CGAGCAACTTTCTTTGCTGC+TGG 0.378507 5.2:+62120437 None:intergenic
TGAAGGGAGTTGATCAAATC+TGG 0.381983 5.2:+62120989 None:intergenic
CTTTCTTTGCTGCTGGGACT+CGG 0.390404 5.2:+62120444 None:intergenic
TATAAAGATTAAGGTCTTGT+TGG 0.392269 5.2:+62120084 None:intergenic
GAATATCCAAAGGTGAATAA+TGG 0.394530 5.2:-62120320 MS.gene99722:CDS
CTGATGGAAGCCCTTCCATT+TGG 0.396769 5.2:-62120628 MS.gene99722:CDS
TCATGAAACCGCGAAGTTCA+TGG 0.399822 5.2:-62120969 MS.gene99722:CDS
ACAGATTAGGAGAAGCAATC+AGG 0.400494 5.2:-62120499 MS.gene99722:CDS
AACTCATCGATCATGATGTT+AGG 0.400629 5.2:+62121016 None:intergenic
TGTATATGATAAGAAGCTTA+AGG 0.401177 5.2:-62119904 MS.gene99722:CDS
CCAATGAAGATTCCATATAC+TGG 0.407422 5.2:+62119846 None:intergenic
TGTACAGAGTTCAGTCGAAT+TGG 0.407634 5.2:-62119929 MS.gene99722:CDS
CCAGTATATGGAATCTTCAT+TGG 0.414153 5.2:-62119846 MS.gene99722:CDS
GTTTCTGATCGAAAGCAGTT+AGG 0.430631 5.2:-62119739 None:intergenic
TCACAGAGTCGTTGTTCAAA+TGG 0.432437 5.2:+62120749 None:intergenic
GAGTTTGTCGCGGCAAACTC+AGG 0.438381 5.2:-62121386 MS.gene99722:CDS
CAGTTATTCATTATTGGAGC+AGG 0.449950 5.2:-62121292 MS.gene99722:CDS
AGAAGATTTAATTAAAAGGT+GGG 0.450847 5.2:-62120159 MS.gene99722:CDS
GTTACAGTCCAAAACAGATT+AGG 0.467068 5.2:-62120512 MS.gene99722:CDS
AATTTCTTCTACAGAAGAAA+AGG 0.469100 5.2:+62121237 None:intergenic
CCATTGCAGAAGGAAATGTT+TGG 0.471945 5.2:-62120906 MS.gene99722:CDS
GCGAAGTTCATGGTAAGTGC+TGG 0.476769 5.2:-62120959 MS.gene99722:CDS
AAACCATATTTGGTAACGAT+TGG 0.478817 5.2:-62120130 MS.gene99722:CDS
GAGCAACTTTCTTTGCTGCT+GGG 0.482217 5.2:+62120438 None:intergenic
TTGAATAAGCACCAAATGGA+AGG 0.483668 5.2:+62120617 None:intergenic
AAGCGACGTAGCTAATGATC+TGG 0.484649 5.2:+62120384 None:intergenic
CATGGTAAGTGCTGGGTTTG+AGG 0.484781 5.2:-62120951 MS.gene99722:CDS
CCAAACATTTCCTTCTGCAA+TGG 0.491403 5.2:+62120906 None:intergenic
GGAAGGTTCCGTGATTGTCT+TGG 0.497009 5.2:-62119825 MS.gene99722:CDS
CTAATGATCTGGACCACCAT+TGG 0.498577 5.2:+62120395 None:intergenic
TTCGCTGATGCAGTTGCTGA+TGG 0.507020 5.2:-62120644 MS.gene99722:CDS
TCTGATGCCACATCATCATT+GGG 0.515118 5.2:+62119999 None:intergenic
AAGAAAGTTGCTCGGTCTGA+TGG 0.517438 5.2:-62120428 MS.gene99722:CDS
ACTCATCGATCATGATGTTA+GGG 0.526299 5.2:+62121017 None:intergenic
TATATGGAATCTTCATTGGA+AGG 0.529034 5.2:-62119842 MS.gene99722:CDS
CGAAGTTCATGGTAAGTGCT+GGG 0.535756 5.2:-62120958 MS.gene99722:CDS
CAGATTAGGAGAAGCAATCA+GGG 0.537526 5.2:-62120498 MS.gene99722:CDS
GGGTGCTGATTCTTCGGTGA+AGG 0.537582 5.2:-62120543 MS.gene99722:CDS
GAACTTCGCGGTTTCATGAA+GGG 0.539523 5.2:+62120973 None:intergenic
TGAACTTCGCGGTTTCATGA+AGG 0.542330 5.2:+62120972 None:intergenic
ACATCGGGAATGAAAATCAA+AGG 0.543866 5.2:-62120841 MS.gene99722:CDS
TGAATAAGCACCAAATGGAA+GGG 0.545777 5.2:+62120618 None:intergenic
TAGAAGATTTAATTAAAAGG+TGG 0.546097 5.2:-62120160 MS.gene99722:CDS
TCGATCATGATGTTAGGGCC+AGG 0.553016 5.2:+62121022 None:intergenic
TTGCAGAAGGAAATGTTTGG+AGG 0.555339 5.2:-62120903 MS.gene99722:CDS
CGATCAGAAACAAATTGTTG+AGG 0.556368 5.2:+62119751 None:intergenic
GATTGCAGAAGATTAACATC+GGG 0.561302 5.2:-62120856 MS.gene99722:CDS
GCTACGTCGCTTCTGCATGT+AGG 0.571994 5.2:-62120373 MS.gene99722:CDS
TGAAAAGATTGCGCAAAGCA+AGG 0.573006 5.2:+62120204 None:intergenic
CATCGGGAATGAAAATCAAA+GGG 0.583551 5.2:-62120840 MS.gene99722:CDS
AGCACTTACCATGAACTTCG+CGG 0.585018 5.2:+62120961 None:intergenic
ATGTATACATCCATTGCAGA+AGG 0.591121 5.2:-62120916 MS.gene99722:CDS
AGTTTGTCGCGGCAAACTCA+GGG 0.593964 5.2:-62121385 MS.gene99722:CDS
GGAAGAAATAATAATATCAG+TGG 0.606646 5.2:-62119883 MS.gene99722:CDS
TTGATATCGAAATTCCACTT+GGG 0.612668 5.2:-62120563 MS.gene99722:CDS
TTTGATATCGAAATTCCACT+TGG 0.613822 5.2:-62120564 MS.gene99722:CDS
AAACAACGAGTAGCAGAGGA+GGG 0.616280 5.2:+62120707 None:intergenic
AACTCAGGGTGGATTCGAAG+TGG 0.619524 5.2:-62121371 MS.gene99722:CDS
CAGCAGCAAAGAAAGTTGCT+CGG 0.622781 5.2:-62120436 MS.gene99722:CDS
TGATGGTCGACATCATCCAA+TGG 0.628831 5.2:-62120411 MS.gene99722:CDS
AATGAATACCAAGACAATCA+CGG 0.629260 5.2:+62119817 None:intergenic
CCGAAGAATCAGCACCCAAG+TGG 0.635114 5.2:+62120549 None:intergenic
GATTCCATATACTGGCAGCA+AGG 0.639874 5.2:+62119854 None:intergenic
GAAACAACGAGTAGCAGAGG+AGG 0.648023 5.2:+62120706 None:intergenic
GGATTGCAGAAGATTAACAT+CGG 0.649736 5.2:-62120857 MS.gene99722:CDS
TTGTCGCGGCAAACTCAGGG+TGG 0.652057 5.2:-62121382 MS.gene99722:CDS
TGTGAAACAACGAGTAGCAG+AGG 0.656858 5.2:+62120703 None:intergenic
TCTGGCACCCAATGATGATG+TGG 0.663170 5.2:-62120006 MS.gene99722:CDS
TGCTACTCGTTGTTTCACAG+AGG 0.669048 5.2:-62120699 MS.gene99722:CDS
GAAGCTGAAACGTTGCTCCG+TGG 0.670987 5.2:+62120672 None:intergenic
GCAAAACTTGTTCTAGCGTG+CGG 0.672133 5.2:+62120345 None:intergenic
AACAACGAGTAGCAGAGGAG+GGG 0.675729 5.2:+62120708 None:intergenic
TCATGTCACTGAGTTTGTCG+CGG 0.677273 5.2:-62121396 MS.gene99722:CDS
TGGTCGACATCATCCAATGG+TGG 0.692831 5.2:-62120408 MS.gene99722:CDS
AGGGAGTTGATCAAATCTGG+AGG 0.695828 5.2:+62120992 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACATAGAAGATTTAATTAAA+AGG - chr5.2:62120971-62120990 MS.gene99722:CDS 15.0%
!! ATCTTTATAAAAAAAAATCG+TGG - chr5.2:62121064-62121083 MS.gene99722:CDS 15.0%
!! TAGATATTGTGATTAAAAAA+TGG - chr5.2:62120326-62120345 MS.gene99722:CDS 15.0%
!! AGAAGATTTAATTAAAAGGT+GGG - chr5.2:62120975-62120994 MS.gene99722:CDS 20.0%
!! TAAAAAAAAATCGTGGAATA+TGG - chr5.2:62121071-62121090 MS.gene99722:CDS 20.0%
!! TAGAAGATTTAATTAAAAGG+TGG - chr5.2:62120974-62120993 MS.gene99722:CDS 20.0%
!! TCTAATCAGTATATTAGATA+TGG - chr5.2:62121339-62121358 MS.gene99722:CDS 20.0%
!! TGTCTAATCAATAGATTATT+CGG - chr5.2:62120256-62120275 MS.gene99722:CDS 20.0%
!!! ATCTTTTCATGATGAATAAT+AGG - chr5.2:62120944-62120963 MS.gene99722:CDS 20.0%
!!! GATAATTTAGTTTCAAGAAT+AGG + chr5.2:62120663-62120682 None:intergenic 20.0%
! AAGTTGCAGTTATTCATTAT+TGG - chr5.2:62119836-62119855 MS.gene99722:CDS 25.0%
! AATTTCTTCTACAGAAGAAA+AGG + chr5.2:62119900-62119919 None:intergenic 25.0%
! GGAAGAAATAATAATATCAG+TGG - chr5.2:62121251-62121270 MS.gene99722:CDS 25.0%
! GGAAGAATGAAAAATGATAA+CGG + chr5.2:62119879-62119898 None:intergenic 25.0%
! TGAATTCCAAGATGAAAATT+CGG - chr5.2:62119928-62119947 MS.gene99722:CDS 25.0%
! TGTATATGATAAGAAGCTTA+AGG - chr5.2:62121230-62121249 MS.gene99722:CDS 25.0%
! TTTAATTAAAAGGTGGGATT+TGG - chr5.2:62120981-62121000 MS.gene99722:CDS 25.0%
!! CTTTTATACAACAGATGAAA+TGG - chr5.2:62120854-62120873 MS.gene99722:CDS 25.0%
!! TAATTACCGAATTTTCATCT+TGG + chr5.2:62119937-62119956 None:intergenic 25.0%
!! TATAAAGATTAAGGTCTTGT+TGG + chr5.2:62121053-62121072 None:intergenic 25.0%
!! TTTTCATGATGAATAATAGG+AGG - chr5.2:62120947-62120966 MS.gene99722:CDS 25.0%
!! TTTTCTTCTGTAGAAGAAAT+TGG - chr5.2:62119899-62119918 MS.gene99722:CDS 25.0%
!!! GATCTTCTTTACTTTTTTCT+CGG - chr5.2:62119785-62119804 MS.gene99722:CDS 25.0%
AATGAATACCAAGACAATCA+CGG + chr5.2:62121320-62121339 None:intergenic 30.0%
ACAACAGATGAAATGGATAA+TGG - chr5.2:62120861-62120880 MS.gene99722:CDS 30.0%
GAATATCCAAAGGTGAATAA+TGG - chr5.2:62120814-62120833 MS.gene99722:CDS 30.0%
TATATGGAATCTTCATTGGA+AGG - chr5.2:62121292-62121311 MS.gene99722:CDS 30.0%
TTGATATCGAAATTCCACTT+GGG - chr5.2:62120571-62120590 MS.gene99722:CDS 30.0%
TTTGATATCGAAATTCCACT+TGG - chr5.2:62120570-62120589 MS.gene99722:CDS 30.0%
! CATTTTGAATAAGCACCAAA+TGG + chr5.2:62120524-62120543 None:intergenic 30.0%
! GGAATTTTGCTTGATTTTTC+TGG + chr5.2:62121032-62121051 None:intergenic 30.0%
!! AAACCATATTTGGTAACGAT+TGG - chr5.2:62121004-62121023 MS.gene99722:CDS 30.0%
AACTCATCGATCATGATGTT+AGG + chr5.2:62120121-62120140 None:intergenic 35.0%
ACATCGGGAATGAAAATCAA+AGG - chr5.2:62120293-62120312 MS.gene99722:CDS 35.0%
ACTACTCCATTATTCACCTT+TGG + chr5.2:62120823-62120842 None:intergenic 35.0%
ACTCATCGATCATGATGTTA+GGG + chr5.2:62120120-62120139 None:intergenic 35.0%
ATGTATACATCCATTGCAGA+AGG - chr5.2:62120218-62120237 MS.gene99722:CDS 35.0%
CAGTTATTCATTATTGGAGC+AGG - chr5.2:62119842-62119861 MS.gene99722:CDS 35.0%
CATCGGGAATGAAAATCAAA+GGG - chr5.2:62120294-62120313 MS.gene99722:CDS 35.0%
CCAATGAAGATTCCATATAC+TGG + chr5.2:62121291-62121310 None:intergenic 35.0%
CCAGTATATGGAATCTTCAT+TGG - chr5.2:62121288-62121307 MS.gene99722:CDS 35.0%
CGATCAGAAACAAATTGTTG+AGG + chr5.2:62121386-62121405 None:intergenic 35.0%
GAACCAATCGTTACCAAATA+TGG + chr5.2:62121010-62121029 None:intergenic 35.0%
GATTGCAGAAGATTAACATC+GGG - chr5.2:62120278-62120297 MS.gene99722:CDS 35.0%
GGATTGCAGAAGATTAACAT+CGG - chr5.2:62120277-62120296 MS.gene99722:CDS 35.0%
GTTACAGTCCAAAACAGATT+AGG - chr5.2:62120622-62120641 MS.gene99722:CDS 35.0%
TGAATAAGCACCAAATGGAA+GGG + chr5.2:62120519-62120538 None:intergenic 35.0%
TGGGATTTGGAAACCATATT+TGG - chr5.2:62120994-62121013 MS.gene99722:CDS 35.0%
TTGAATAAGCACCAAATGGA+AGG + chr5.2:62120520-62120539 None:intergenic 35.0%
! AGTTTTGCAGGAATATCCAA+AGG - chr5.2:62120804-62120823 MS.gene99722:CDS 35.0%
! TTGCTTCTCCTAATCTGTTT+TGG + chr5.2:62120633-62120652 None:intergenic 35.0%
!!! GGTGTTTGAGTTTTTGAAGT+TGG - chr5.2:62121092-62121111 MS.gene99722:CDS 35.0%
AATACCTTGCTGCCAGTATA+TGG - chr5.2:62121276-62121295 MS.gene99722:CDS 40.0%
ACAGATTAGGAGAAGCAATC+AGG - chr5.2:62120635-62120654 MS.gene99722:CDS 40.0%
CAGATTAGGAGAAGCAATCA+GGG - chr5.2:62120636-62120655 MS.gene99722:CDS 40.0%
CCAAACATTTCCTTCTGCAA+TGG + chr5.2:62120231-62120250 None:intergenic 40.0%
CCATTGCAGAAGGAAATGTT+TGG - chr5.2:62120228-62120247 MS.gene99722:CDS 40.0%
TCACAGAGTCGTTGTTCAAA+TGG + chr5.2:62120388-62120407 None:intergenic 40.0%
TCTGATGCCACATCATCATT+GGG + chr5.2:62121138-62121157 None:intergenic 40.0%
TGAAAAGATTGCGCAAAGCA+AGG + chr5.2:62120933-62120952 None:intergenic 40.0%
TGTACAGAGTTCAGTCGAAT+TGG - chr5.2:62121205-62121224 MS.gene99722:CDS 40.0%
TTGCAGAAGGAAATGTTTGG+AGG - chr5.2:62120231-62120250 MS.gene99722:CDS 40.0%
! CTCTGTGATCATGTCTTTTC+AGG - chr5.2:62120400-62120419 MS.gene99722:CDS 40.0%
! TGAAGGGAGTTGATCAAATC+TGG + chr5.2:62120148-62120167 None:intergenic 40.0%
! TGTTTCACAGAGGTTTTCCA+CGG - chr5.2:62120445-62120464 MS.gene99722:CDS 40.0%
AAACAACGAGTAGCAGAGGA+GGG + chr5.2:62120430-62120449 None:intergenic 45.0%
AAGAAAGTTGCTCGGTCTGA+TGG - chr5.2:62120706-62120725 MS.gene99722:CDS 45.0%
AAGCGACGTAGCTAATGATC+TGG + chr5.2:62120753-62120772 None:intergenic 45.0%
AGCAAGGTCTCTGTACTCTT+TGG + chr5.2:62120917-62120936 None:intergenic 45.0%
AGCACTTACCATGAACTTCG+CGG + chr5.2:62120176-62120195 None:intergenic 45.0%
CAGCAGCAAAGAAAGTTGCT+CGG - chr5.2:62120698-62120717 MS.gene99722:CDS 45.0%
CGAAGTTCATGGTAAGTGCT+GGG - chr5.2:62120176-62120195 MS.gene99722:CDS 45.0%
CTAATGATCTGGACCACCAT+TGG + chr5.2:62120742-62120761 None:intergenic 45.0%
CTCTGATGCCACATCATCAT+TGG + chr5.2:62121139-62121158 None:intergenic 45.0%
GAACTTCGCGGTTTCATGAA+GGG + chr5.2:62120164-62120183 None:intergenic 45.0%
GAGCAACTTTCTTTGCTGCT+GGG + chr5.2:62120699-62120718 None:intergenic 45.0%
GATTCCATATACTGGCAGCA+AGG + chr5.2:62121283-62121302 None:intergenic 45.0%
GTTGGACAACAGTGACTATC+TGG - chr5.2:62121110-62121129 MS.gene99722:CDS 45.0%
TCATGAAACCGCGAAGTTCA+TGG - chr5.2:62120165-62120184 MS.gene99722:CDS 45.0%
TGAACTTCGCGGTTTCATGA+AGG + chr5.2:62120165-62120184 None:intergenic 45.0%
TGATGGTCGACATCATCCAA+TGG - chr5.2:62120723-62120742 MS.gene99722:CDS 45.0%
TGTGAAACAACGAGTAGCAG+AGG + chr5.2:62120434-62120453 None:intergenic 45.0%
! AGGGAGTTGATCAAATCTGG+AGG + chr5.2:62120145-62120164 None:intergenic 45.0%
! CACGCTAGAACAAGTTTTGC+AGG - chr5.2:62120792-62120811 MS.gene99722:CDS 45.0%
! GACTCTGATTTGATGTTGCC+TGG - chr5.2:62120094-62120113 MS.gene99722:CDS 45.0%
! GCAAAACTTGTTCTAGCGTG+CGG + chr5.2:62120792-62120811 None:intergenic 45.0%
! TGCTACTCGTTGTTTCACAG+AGG - chr5.2:62120435-62120454 MS.gene99722:CDS 45.0%
!!! ATTTTTTTTTATAAAGATTA+AGG + chr5.2:62121062-62121081 None:intergenic 5.0%
AACAACGAGTAGCAGAGGAG+GGG + chr5.2:62120429-62120448 None:intergenic 50.0%
AGTTTGTCGCGGCAAACTCA+GGG - chr5.2:62119749-62119768 MS.gene99722:CDS 50.0%
CGAGCAACTTTCTTTGCTGC+TGG + chr5.2:62120700-62120719 None:intergenic 50.0%
CTGATGGAAGCCCTTCCATT+TGG - chr5.2:62120506-62120525 MS.gene99722:CDS 50.0%
CTTTCTTTGCTGCTGGGACT+CGG + chr5.2:62120693-62120712 None:intergenic 50.0%
GAAACAACGAGTAGCAGAGG+AGG + chr5.2:62120431-62120450 None:intergenic 50.0%
GCGAAGTTCATGGTAAGTGC+TGG - chr5.2:62120175-62120194 MS.gene99722:CDS 50.0%
GGAAGGTTCCGTGATTGTCT+TGG - chr5.2:62121309-62121328 MS.gene99722:CDS 50.0%
TCGATCATGATGTTAGGGCC+AGG + chr5.2:62120115-62120134 None:intergenic 50.0%
TGGTCGACATCATCCAATGG+TGG - chr5.2:62120726-62120745 MS.gene99722:CDS 50.0%
TTCGCTGATGCAGTTGCTGA+TGG - chr5.2:62120490-62120509 MS.gene99722:CDS 50.0%
! AACTCAGGGTGGATTCGAAG+TGG - chr5.2:62119763-62119782 MS.gene99722:CDS 50.0%
! TCTGGCACCCAATGATGATG+TGG - chr5.2:62121128-62121147 MS.gene99722:CDS 50.0%
!! CATGGTAAGTGCTGGGTTTG+AGG - chr5.2:62120183-62120202 MS.gene99722:CDS 50.0%
!! CCACTTGGGTGCTGATTCTT+CGG - chr5.2:62120585-62120604 MS.gene99722:CDS 50.0%
CCGAAGAATCAGCACCCAAG+TGG + chr5.2:62120588-62120607 None:intergenic 55.0%
GAAGCTGAAACGTTGCTCCG+TGG + chr5.2:62120465-62120484 None:intergenic 55.0%
GAGTTTGTCGCGGCAAACTC+AGG - chr5.2:62119748-62119767 MS.gene99722:CDS 55.0%
GCTACGTCGCTTCTGCATGT+AGG - chr5.2:62120761-62120780 MS.gene99722:CDS 55.0%
!! GGGTGCTGATTCTTCGGTGA+AGG - chr5.2:62120591-62120610 MS.gene99722:CDS 55.0%
TTGTCGCGGCAAACTCAGGG+TGG - chr5.2:62119752-62119771 MS.gene99722:CDS 60.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 62119740 62121416 62119740 ID=MS.gene99722
chr5.2 mRNA 62119740 62121416 62119740 ID=MS.gene99722.t1;Parent=MS.gene99722
chr5.2 exon 62119740 62121416 62119740 ID=MS.gene99722.t1.exon1;Parent=MS.gene99722.t1
chr5.2 CDS 62119740 62121416 62119740 ID=cds.MS.gene99722.t1;Parent=MS.gene99722.t1
Gene Sequence

>MS.gene99722

ATGTCACTGAGTTTGTCGCGGCAAACTCAGGGTGGATTCGAAGTGGATCTTCTTTACTTTTTTCTCGGATGTCTAACTATGTTACTCATGAAGATTAAGTTGCAGTTATTCATTATTGGAGCAGGTTTGAGTTACTCCGTTATCATTTTTCATTCTTCCTTTTCTTCTGTAGAAGAAATTGGATTCTTTGAATTCCAAGATGAAAATTCGGTAATTATTGATGTAGATTCGCAACAACTAGCTAATACTGATATTGCTAGTACGATTGAACAACTAAAAAATTATCTTAACATGCTTCAGCAGAAAAATTTGAACATCGTCGACATGCTTTTGAAGCATGTGAATGAATATGATGACTCTGATTTGATGTTGCCTGGCCCTAACATCATGATCGATGAGTTGCCTCCAGATTTGATCAACTCCCTTCATGAAACCGCGAAGTTCATGGTAAGTGCTGGGTTTGAGGATGAGTTTTCTAATGTATACATCCATTGCAGAAGGAAATGTTTGGAGGAGTGTCTAATCAATAGATTATTCGGATTGCAGAAGATTAACATCGGGAATGAAAATCAAAGGGCGAGATATGTAGATATTGTGATTAAAAAATGGATCACAGCATCAGAAATCGCTCTTAAGATTCTATTTCCATTTGAACAACGACTCTGTGATCATGTCTTTTCAGGTTTCCCCTCCTCTGCTACTCGTTGTTTCACAGAGGTTTTCCACGGAGCAACGTTTCAGCTTCTGAATTTCGCTGATGCAGTTGCTGATGGAAGCCCTTCCATTTGGTGCTTATTCAAAATGCTCGCAGTCTTTGAGACATTGCATTATTTGATATCGAAATTCCACTTGGGTGCTGATTCTTCGGTGAAGGAAGCTGCAGTTACAGTCCAAAACAGATTAGGAGAAGCAATCAGGGACCTATTCTTGAAACTAAATTATCTGACTTTCCGAGTCCCAGCAGCAAAGAAAGTTGCTCGGTCTGATGGTCGACATCATCCAATGGTGGTCCAGATCATTAGCTACGTCGCTTCTGCATGTAGGTCACGCCGCACGCTAGAACAAGTTTTGCAGGAATATCCAAAGGTGAATAATGGAGTAGTACTGAAAGAATCTTTTATACAACAGATGAAATGGATAATGGATATGTTAGAGAAAAGATTGAACTGTAAATCCAAAGAGTACAGAGACCTTGCTTTGCGCAATCTTTTCATGATGAATAATAGGAGGCACATAGAAGATTTAATTAAAAGGTGGGATTTGGAAACCATATTTGGTAACGATTGGTTCCAGAAAAATCAAGCAAAATTCCAACAAGACCTTAATCTTTATAAAAAAAAATCGTGGAATATGGTGTTTGAGTTTTTGAAGTTGGACAACAGTGACTATCTGGCACCCAATGATGATGTGGCATCAGAGTTTATGAAAGATAAGCTAAAATTGTTCAACAAGCACTTTGAAGAGATGTACAGAGTTCAGTCGAATTGGTTTGTATATGATAAGAAGCTTAAGGAAGAAATAATAATATCAGTGGCAAATACCTTGCTGCCAGTATATGGAATCTTCATTGGAAGGTTCCGTGATTGTCTTGGTATTCATTCTAATCAGTATATTAGATATGGAATGTTTGAGATTCAAGATCGCCTCAACAATTTGTTTCTGATCGAAAGCAGTTAG
Protein sequence

>MS.gene99722.t1

MSLSLSRQTQGGFEVDLLYFFLGCLTMLLMKIKLQLFIIGAGLSYSVIIFHSSFSSVEEIGFFEFQDENSVIIDVDSQQLANTDIASTIEQLKNYLNMLQQKNLNIVDMLLKHVNEYDDSDLMLPGPNIMIDELPPDLINSLHETAKFMVSAGFEDEFSNVYIHCRRKCLEECLINRLFGLQKINIGNENQRARYVDIVIKKWITASEIALKILFPFEQRLCDHVFSGFPSSATRCFTEVFHGATFQLLNFADAVADGSPSIWCLFKMLAVFETLHYLISKFHLGADSSVKEAAVTVQNRLGEAIRDLFLKLNYLTFRVPAAKKVARSDGRHHPMVVQIISYVASACRSRRTLEQVLQEYPKVNNGVVLKESFIQQMKWIMDMLEKRLNCKSKEYRDLALRNLFMMNNRRHIEDLIKRWDLETIFGNDWFQKNQAKFQQDLNLYKKKSWNMVFEFLKLDNSDYLAPNDDVASEFMKDKLKLFNKHFEEMYRVQSNWFVYDKKLKEEIIISVANTLLPVYGIFIGRFRDCLGIHSNQYIRYGMFEIQDRLNNLFLIESS