Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080047817.01.T01 | XP_013453150.1 | 95.775 | 142 | 6 | 0 | 1 | 142 | 1 | 142 | 6.51E-97 | 287 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080047817.01.T01 | P59082 | 32.812 | 128 | 79 | 2 | 10 | 135 | 20 | 142 | 5.13E-20 | 84.3 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080047817.01.T01 | I3T927 | 95.775 | 142 | 6 | 0 | 1 | 142 | 1 | 142 | 3.11e-97 | 287 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047817.01 | MsG0480019034.01 | 0.818696 | 1.712789e-52 | 1.291209e-49 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080047817.01.T01 | MTR_6g088475 | 95.775 | 142 | 6 | 0 | 1 | 142 | 1 | 142 | 7.88e-101 | 287 |
| MsG0080047817.01.T01 | MTR_6g088445 | 83.217 | 143 | 24 | 0 | 1 | 143 | 1 | 143 | 1.27e-89 | 258 |
| MsG0080047817.01.T01 | MTR_3g109880 | 40.645 | 155 | 77 | 4 | 5 | 145 | 3 | 156 | 6.02e-35 | 120 |
| MsG0080047817.01.T01 | MTR_1g046660 | 37.762 | 143 | 81 | 2 | 5 | 141 | 3 | 143 | 2.09e-30 | 108 |
| MsG0080047817.01.T01 | MTR_5g081780 | 27.068 | 133 | 92 | 3 | 3 | 134 | 2 | 130 | 3.18e-13 | 63.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080047817.01.T01 | AT2G25770 | 45.255 | 137 | 73 | 2 | 6 | 141 | 5 | 140 | 1.52e-40 | 134 |
| MsG0080047817.01.T01 | AT2G25770 | 45.255 | 137 | 73 | 2 | 6 | 141 | 5 | 140 | 1.52e-40 | 134 |
| MsG0080047817.01.T01 | AT4G32870 | 40.559 | 143 | 73 | 3 | 10 | 145 | 9 | 146 | 3.56e-33 | 115 |
Find 39 sgRNAs with CRISPR-Local
Find 46 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCATCCTACTAATTCTGTTT+TGG | 0.228223 | contig113end:-30777 | None:intergenic |
| ATTCCTATGTTTCAACTGTT+AGG | 0.254699 | contig113end:+31053 | MsG0080047817.01.T01:CDS |
| ATCTCACAACCATTATCTTT+TGG | 0.281282 | contig113end:-31082 | None:intergenic |
| TAGCTAAACACCCTTTGAAT+TGG | 0.351254 | contig113end:-31001 | None:intergenic |
| TCTTGATTATTCATTGGTTC+AGG | 0.355434 | contig113end:+31153 | MsG0080047817.01.T01:CDS |
| TTCCTATGTTTCAACTGTTA+GGG | 0.363254 | contig113end:+31054 | MsG0080047817.01.T01:CDS |
| TCGGACACAACCGGGCTTGC+CGG | 0.369834 | contig113end:-30894 | None:intergenic |
| TTTAGCTATGCAATTGTTGA+TGG | 0.381117 | contig113end:+31016 | MsG0080047817.01.T01:CDS |
| TTGTTTGCCATGTTATCAAC+TGG | 0.383024 | contig113end:-30938 | None:intergenic |
| GGAGTATCTTGATTATTCAT+TGG | 0.397480 | contig113end:+31147 | MsG0080047817.01.T01:CDS |
| TGGTTCAGGCTTGGATGAGA+TGG | 0.404366 | contig113end:+31167 | MsG0080047817.01.T01:CDS |
| ATTATTCATTGGTTCAGGCT+TGG | 0.407895 | contig113end:+31158 | MsG0080047817.01.T01:CDS |
| GGCACAGAATCGGACACAAC+CGG | 0.409965 | contig113end:-30903 | None:intergenic |
| TCGTATTGCATTCCAGTTGA+AGG | 0.421146 | contig113end:+30866 | MsG0080047817.01.T01:CDS |
| ACATGTTGAAGGGTGGAGAT+TGG | 0.438884 | contig113end:+31126 | MsG0080047817.01.T01:CDS |
| TTCAATTGATCCAATTCAAA+GGG | 0.444390 | contig113end:+30991 | MsG0080047817.01.T01:CDS |
| TTGCCGGATATTCCTTCAAC+TGG | 0.447823 | contig113end:-30878 | None:intergenic |
| GCAATTGTTGATGGAAATGT+TGG | 0.448397 | contig113end:+31025 | MsG0080047817.01.T01:CDS |
| GATGCAAAGCTGACCAAGTA+TGG | 0.471506 | contig113end:+30795 | MsG0080047817.01.T01:CDS |
| TCTTGAAACCGGCACAGAAT+CGG | 0.477023 | contig113end:-30913 | None:intergenic |
| CCAAGTATGGTCTCTCTTGG+AGG | 0.482137 | contig113end:+30808 | MsG0080047817.01.T01:CDS |
| TGACCAAGTATGGTCTCTCT+TGG | 0.484320 | contig113end:+30805 | MsG0080047817.01.T01:CDS |
| CCTCCAAGAGAGACCATACT+TGG | 0.486375 | contig113end:-30808 | None:intergenic |
| TTTCAATTGATCCAATTCAA+AGG | 0.496174 | contig113end:+30990 | MsG0080047817.01.T01:CDS |
| ATTCCAGTTGAAGGAATATC+CGG | 0.498959 | contig113end:+30875 | MsG0080047817.01.T01:CDS |
| ATCAACTGGAGTCTTGAAAC+CGG | 0.505621 | contig113end:-30924 | None:intergenic |
| GGTTGTGTCCGATTCTGTGC+CGG | 0.509544 | contig113end:+30905 | MsG0080047817.01.T01:CDS |
| TATGAAGTTGAACATGTTGA+AGG | 0.517153 | contig113end:+31115 | MsG0080047817.01.T01:CDS |
| GAAGGAATATCCGGCAAGCC+CGG | 0.523372 | contig113end:+30884 | MsG0080047817.01.T01:CDS |
| CAAGACTCCAGTTGATAACA+TGG | 0.535461 | contig113end:+30931 | MsG0080047817.01.T01:CDS |
| GGTTCAGGCTTGGATGAGAT+GGG | 0.542743 | contig113end:+31168 | MsG0080047817.01.T01:CDS |
| AAGGCCAAAACAGAATTAGT+AGG | 0.542967 | contig113end:+30773 | MsG0080047817.01.T01:CDS |
| GCACAGAATCGGACACAACC+GGG | 0.564414 | contig113end:-30902 | None:intergenic |
| AACCCTAACAGTTGAAACAT+AGG | 0.579967 | contig113end:-31056 | None:intergenic |
| GAGATGGGTCAGAGAATGCA+AGG | 0.589459 | contig113end:+31183 | MsG0080047817.01.T01:CDS |
| ATAATGGTTGTGAGATTGAG+TGG | 0.604758 | contig113end:+31089 | MsG0080047817.01.T01:CDS |
| GGAATGCAATACGAGAGAGT+TGG | 0.605293 | contig113end:-30857 | None:intergenic |
| AAGTTGAACATGTTGAAGGG+TGG | 0.641637 | contig113end:+31119 | MsG0080047817.01.T01:CDS |
| ATGAAGTTGAACATGTTGAA+GGG | 0.669637 | contig113end:+31116 | MsG0080047817.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTCAACAAACAAAAGAAAAA+TGG | + | contig113end:30744-30763 | MsG0080047817.01.T01:CDS | 20.0% |
| !!! | ATTTTTTTGGTCTTGATAAA+TGG | + | contig113end:30831-30850 | MsG0080047817.01.T01:CDS | 20.0% |
| ! | TTCAATTGATCCAATTCAAA+GGG | + | contig113end:30991-31010 | MsG0080047817.01.T01:CDS | 25.0% |
| ! | TTTCAATTGATCCAATTCAA+AGG | + | contig113end:30990-31009 | MsG0080047817.01.T01:CDS | 25.0% |
| ATGAAGTTGAACATGTTGAA+GGG | + | contig113end:31116-31135 | MsG0080047817.01.T01:CDS | 30.0% | |
| ATTCCTATGTTTCAACTGTT+AGG | + | contig113end:31053-31072 | MsG0080047817.01.T01:CDS | 30.0% | |
| CAAACAAAAGAAAAATGGCA+AGG | + | contig113end:30749-30768 | MsG0080047817.01.T01:CDS | 30.0% | |
| TATGAAGTTGAACATGTTGA+AGG | + | contig113end:31115-31134 | MsG0080047817.01.T01:CDS | 30.0% | |
| TGGCAAACAAAAACTTGAAT+TGG | + | contig113end:30951-30970 | MsG0080047817.01.T01:CDS | 30.0% | |
| TTCCTATGTTTCAACTGTTA+GGG | + | contig113end:31054-31073 | MsG0080047817.01.T01:CDS | 30.0% | |
| TTTAGCTATGCAATTGTTGA+TGG | + | contig113end:31016-31035 | MsG0080047817.01.T01:CDS | 30.0% | |
| ! | ATCTCACAACCATTATCTTT+TGG | - | contig113end:31085-31104 | None:intergenic | 30.0% |
| ! | GGAGTATCTTGATTATTCAT+TGG | + | contig113end:31147-31166 | MsG0080047817.01.T01:CDS | 30.0% |
| !! | TCTTGATTATTCATTGGTTC+AGG | + | contig113end:31153-31172 | MsG0080047817.01.T01:CDS | 30.0% |
| AAAAGAAAAATGGCAAGGCA+AGG | + | contig113end:30754-30773 | MsG0080047817.01.T01:CDS | 35.0% | |
| AACCCTAACAGTTGAAACAT+AGG | - | contig113end:31059-31078 | None:intergenic | 35.0% | |
| AAGGCCAAAACAGAATTAGT+AGG | + | contig113end:30773-30792 | MsG0080047817.01.T01:CDS | 35.0% | |
| ATAATGGTTGTGAGATTGAG+TGG | + | contig113end:31089-31108 | MsG0080047817.01.T01:CDS | 35.0% | |
| ATTCCAGTTGAAGGAATATC+CGG | + | contig113end:30875-30894 | MsG0080047817.01.T01:CDS | 35.0% | |
| TAGCTAAACACCCTTTGAAT+TGG | - | contig113end:31004-31023 | None:intergenic | 35.0% | |
| TTGTTTGCCATGTTATCAAC+TGG | - | contig113end:30941-30960 | None:intergenic | 35.0% | |
| ! | AGGGTTTTGCCAAAAGATAA+TGG | + | contig113end:31073-31092 | MsG0080047817.01.T01:CDS | 35.0% |
| ! | GCAATTGTTGATGGAAATGT+TGG | + | contig113end:31025-31044 | MsG0080047817.01.T01:CDS | 35.0% |
| ! | GCATCCTACTAATTCTGTTT+TGG | - | contig113end:30780-30799 | None:intergenic | 35.0% |
| !! | ATTATTCATTGGTTCAGGCT+TGG | + | contig113end:31158-31177 | MsG0080047817.01.T01:CDS | 35.0% |
| !!! | TCTCTCTTGGAGGATTTTTT+TGG | + | contig113end:30818-30837 | MsG0080047817.01.T01:CDS | 35.0% |
| AAGTTGAACATGTTGAAGGG+TGG | + | contig113end:31119-31138 | MsG0080047817.01.T01:CDS | 40.0% | |
| ATCAACTGGAGTCTTGAAAC+CGG | - | contig113end:30927-30946 | None:intergenic | 40.0% | |
| TCGTATTGCATTCCAGTTGA+AGG | + | contig113end:30866-30885 | MsG0080047817.01.T01:CDS | 40.0% | |
| !! | CAAGACTCCAGTTGATAACA+TGG | + | contig113end:30931-30950 | MsG0080047817.01.T01:CDS | 40.0% |
| ACATGTTGAAGGGTGGAGAT+TGG | + | contig113end:31126-31145 | MsG0080047817.01.T01:CDS | 45.0% | |
| GATGCAAAGCTGACCAAGTA+TGG | + | contig113end:30795-30814 | MsG0080047817.01.T01:CDS | 45.0% | |
| GGAATGCAATACGAGAGAGT+TGG | - | contig113end:30860-30879 | None:intergenic | 45.0% | |
| TCTTGAAACCGGCACAGAAT+CGG | - | contig113end:30916-30935 | None:intergenic | 45.0% | |
| TGACCAAGTATGGTCTCTCT+TGG | + | contig113end:30805-30824 | MsG0080047817.01.T01:CDS | 45.0% | |
| TTGCCGGATATTCCTTCAAC+TGG | - | contig113end:30881-30900 | None:intergenic | 45.0% | |
| CCAAGTATGGTCTCTCTTGG+AGG | + | contig113end:30808-30827 | MsG0080047817.01.T01:CDS | 50.0% | |
| CCTCCAAGAGAGACCATACT+TGG | - | contig113end:30811-30830 | None:intergenic | 50.0% | |
| GAGATGGGTCAGAGAATGCA+AGG | + | contig113end:31183-31202 | MsG0080047817.01.T01:CDS | 50.0% | |
| GGTTCAGGCTTGGATGAGAT+GGG | + | contig113end:31168-31187 | MsG0080047817.01.T01:CDS | 50.0% | |
| TGGTTCAGGCTTGGATGAGA+TGG | + | contig113end:31167-31186 | MsG0080047817.01.T01:CDS | 50.0% | |
| GAAGGAATATCCGGCAAGCC+CGG | + | contig113end:30884-30903 | MsG0080047817.01.T01:CDS | 55.0% | |
| GCACAGAATCGGACACAACC+GGG | - | contig113end:30905-30924 | None:intergenic | 55.0% | |
| GGCACAGAATCGGACACAAC+CGG | - | contig113end:30906-30925 | None:intergenic | 55.0% | |
| GGTTGTGTCCGATTCTGTGC+CGG | + | contig113end:30905-30924 | MsG0080047817.01.T01:CDS | 55.0% | |
| TCGGACACAACCGGGCTTGC+CGG | - | contig113end:30897-30916 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| contig113end | gene | 30734 | 31213 | 30734 | ID=MsG0080047817.01;Name=MsG0080047817.01 |
| contig113end | mRNA | 30734 | 31213 | 30734 | ID=MsG0080047817.01.T01;Parent=MsG0080047817.01;Name=MsG0080047817.01.T01;_AED=0.48;_eAED=0.48;_QI=0|-1|0|1|-1|1|1|0|159 |
| contig113end | exon | 30734 | 31213 | 30734 | ID=MsG0080047817.01.T01:exon:6239;Parent=MsG0080047817.01.T01 |
| contig113end | CDS | 30734 | 31213 | 30734 | ID=MsG0080047817.01.T01:cds;Parent=MsG0080047817.01.T01 |
| Gene Sequence |
| Protein sequence |
