AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0080047991.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080047991.01.T01 MTR_1g081780 91.582 297 20 2 1 294 1 295 0.0 543
MsG0080047991.01.T01 MTR_1g014445 66.120 183 8 4 104 286 1 129 1.82e-66 204
MsG0080047991.01.T01 MTR_1g111690 53.608 97 41 3 1 94 1 96 1.19e-23 100
MsG0080047991.01.T01 MTR_6g033440 68.421 76 21 2 1 73 1 76 7.16e-22 88.6
MsG0080047991.01.T01 MTR_1g111700 59.459 74 27 2 1 71 1 74 1.93e-19 80.9
MsG0080047991.01.T01 MTR_5g082250 53.968 63 27 1 10 70 14 76 8.84e-14 69.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080047991.01.T01 AT2G40020 44.444 72 39 1 1 71 1 72 7.11e-14 68.9
MsG0080047991.01.T01 AT2G40020 41.772 79 45 1 1 78 1 79 2.10e-13 68.9

Find 108 sgRNAs with CRISPR-Local

Find 216 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACAGTGCCTGCAGCCTTAA+TGG 0.199764 contig162end:-13657 None:intergenic
CATTCATGTAATCGTTATTA+TGG 0.210432 contig162end:-14344 None:intergenic
CCCAATGAGGCATCGGCTTC+TGG 0.268170 contig162end:+13299 MsG0080047991.01.T01:CDS
TTCAACAACAATACTTGTTT+TGG 0.281297 contig162end:+14184 MsG0080047991.01.T01:three_prime_UTR
AAGTAATCAACTTTCCATTA+TGG 0.288420 contig162end:+13535 MsG0080047991.01.T01:CDS
GCCTCATTGGGACCATTTCC+TGG 0.308455 contig162end:-13287 None:intergenic
ATCTTACTTAGTTAAGTCTT+TGG 0.324559 contig162end:+14252 MsG0080047991.01.T01:three_prime_UTR
GTTTGACGAACGAGTTTGTT+AGG 0.357257 contig162end:-11468 None:intergenic
TAGTAATCCACCTTCCATTA+AGG 0.360557 contig162end:+13643 MsG0080047991.01.T01:CDS
TTCAGTGTCTGCGGCCATAA+TGG 0.365812 contig162end:-13549 None:intergenic
ACAAAGTCCTCACAACCTAT+TGG 0.373795 contig162end:+13857 MsG0080047991.01.T01:CDS
AACTTGGTTGTTAATTTCAT+TGG 0.383404 contig162end:-13687 None:intergenic
ACCAGAAGCCGATGCCTCAT+TGG 0.394264 contig162end:-13300 None:intergenic
ATATCAGCAGCCAGTGAAAC+TGG 0.395050 contig162end:+13410 MsG0080047991.01.T01:CDS
CGTCCTCTTGGAGCCATTGT+TGG 0.401386 contig162end:-11295 None:intergenic
AGAACCTTTGGTCGTCCTCT+TGG 0.406725 contig162end:-11307 None:intergenic
TTCTGATGATGAGGAGGAGG+AGG 0.407593 contig162end:+13922 MsG0080047991.01.T01:CDS
TGTGAAGGATTATTTGATAG+AGG 0.411419 contig162end:+13259 MsG0080047991.01.T01:intron
TTTAGGTTTATGACGGTAGT+TGG 0.415465 contig162end:+11611 MsG0080047991.01.T01:intron
CTCCAAGGTTTCAGAATGAT+AGG 0.415644 contig162end:+14082 MsG0080047991.01.T01:three_prime_UTR
TGATGATGAGGAGGAGGAGG+GGG 0.417891 contig162end:+13925 MsG0080047991.01.T01:CDS
TTGTGACAAAGCTTTCCAAT+AGG 0.418635 contig162end:-13872 None:intergenic
CACCTATCATTCTGAAACCT+TGG 0.420580 contig162end:-14084 None:intergenic
TTAATGGAAGGTGGATTACT+AGG 0.428557 contig162end:-13641 None:intergenic
TGAAGTGTCAACGGGCTTGA+TGG 0.435421 contig162end:-13603 None:intergenic
GATGCAGTGTCCACAGTCAT+AGG 0.437332 contig162end:-13443 None:intergenic
GTATCAGCAGACAATGGATA+TGG 0.438141 contig162end:+13734 MsG0080047991.01.T01:CDS
ATAATGGAAAGTTGATTACT+TGG 0.448544 contig162end:-13533 None:intergenic
CCAGAAGCCGATGCCTCATT+GGG 0.450493 contig162end:-13299 None:intergenic
AGTCTCTATAATAGCCTTGA+TGG 0.450701 contig162end:-13711 None:intergenic
TTACTTAGTTAAGTCTTTGG+TGG 0.453658 contig162end:+14255 MsG0080047991.01.T01:three_prime_UTR
ACAACAACAAAATCAGAATC+AGG 0.455434 contig162end:+11687 MsG0080047991.01.T01:CDS
CCTACCTTGCTCTTCTAACA+TGG 0.458449 contig162end:+13343 MsG0080047991.01.T01:CDS
TAATAGCCTTGATGGGAACT+TGG 0.458568 contig162end:-13703 None:intergenic
GCATTGTATAAGCTAGTGAA+TGG 0.460789 contig162end:+13965 MsG0080047991.01.T01:CDS
TGAATTGTTGACGGGCTTGA+TGG 0.462535 contig162end:-13495 None:intergenic
GGTTGTGAGGACTTTGTTTG+AGG 0.464575 contig162end:-13851 None:intergenic
TTTATAATTAAGAAACCAAA+AGG 0.464914 contig162end:-14230 None:intergenic
TATTTGATAGAGGGTAATCC+AGG 0.472005 contig162end:+13269 MsG0080047991.01.T01:CDS
ATAGAGGGTAATCCAGGAAA+TGG 0.475064 contig162end:+13275 MsG0080047991.01.T01:CDS
GAAGTGTCAACGGGCTTGAT+GGG 0.477540 contig162end:-13602 None:intergenic
TGATGCGCCATTACCTAGTC+AGG 0.478335 contig162end:+13376 MsG0080047991.01.T01:CDS
TAGAAGAGCAAGGTAGGAGA+AGG 0.482533 contig162end:-13337 None:intergenic
TGGAAGGTGGATTACTAGGT+TGG 0.486413 contig162end:-13637 None:intergenic
TCAAGTAGTGTTTAAAGAGC+AGG 0.489627 contig162end:+14020 MsG0080047991.01.T01:exon
ATTACTTGGTTGACTGGTGG+CGG 0.490984 contig162end:-13519 None:intergenic
GTGGCGGATGAAGTGTCAAC+GGG 0.492498 contig162end:-13611 None:intergenic
AGGTGGATTACTAGGTTGGC+TGG 0.493754 contig162end:-13633 None:intergenic
GGCGTCGACCTTGCCATGGT+TGG 0.493924 contig162end:+13897 MsG0080047991.01.T01:CDS
GTGGCGGCTGAATTGTTGAC+GGG 0.497692 contig162end:-13503 None:intergenic
TCTGATGATGAGGAGGAGGA+GGG 0.499936 contig162end:+13923 MsG0080047991.01.T01:CDS
CTGATGATGAGGAGGAGGAG+GGG 0.502777 contig162end:+13924 MsG0080047991.01.T01:CDS
GGTGGCGGCTGAATTGTTGA+CGG 0.505104 contig162end:-13504 None:intergenic
AAGTTGATTACTTGGTTGAC+TGG 0.515398 contig162end:-13525 None:intergenic
AAGTTGATGACCAGTTTCAC+TGG 0.517162 contig162end:-13420 None:intergenic
CCACCTTCCATTAAGGCTGC+AGG 0.522608 contig162end:+13650 MsG0080047991.01.T01:CDS
TGGAGCCATTGTTGGAAGGA+AGG 0.522675 contig162end:-11287 None:intergenic
ACAGAAAGGTGAGAGGAGAA+TGG 0.528163 contig162end:+11410 MsG0080047991.01.T01:intron
TTCACTAGCTTATACAATGC+AGG 0.529881 contig162end:-13962 None:intergenic
ATTACTAGGTTGGCTGGTGG+CGG 0.531150 contig162end:-13627 None:intergenic
GATTTCTGATGATGAGGAGG+AGG 0.533613 contig162end:+13919 MsG0080047991.01.T01:CDS
CATTGTATAAGCTAGTGAAT+GGG 0.534796 contig162end:+13966 MsG0080047991.01.T01:CDS
GAATTGTTGACGGGCTTGAT+GGG 0.535292 contig162end:-13494 None:intergenic
TCCCCTGTGTCTAGAATGCA+TGG 0.535848 contig162end:+14123 MsG0080047991.01.T01:three_prime_UTR
TGAGAACTGAGAATGTAGAA+AGG 0.536692 contig162end:-11264 None:intergenic
CCATGTTAGAAGAGCAAGGT+AGG 0.538702 contig162end:-13343 None:intergenic
ACTAGGTAATGGCGCATCAT+CGG 0.541244 contig162end:-13372 None:intergenic
GTTGTATCAGATTAGCTCCA+AGG 0.542792 contig162end:+14067 MsG0080047991.01.T01:three_prime_UTR
CCAACAGCCTGACTAGGTAA+TGG 0.545720 contig162end:-13383 None:intergenic
CTCTTGGAGCCATTGTTGGA+AGG 0.547511 contig162end:-11291 None:intergenic
TACTTTGAGCAATTGATCAA+CGG 0.547765 contig162end:-11490 None:intergenic
GTGAAGGATTATTTGATAGA+GGG 0.553277 contig162end:+13260 MsG0080047991.01.T01:intron
CCATTACCTAGTCAGGCTGT+TGG 0.557254 contig162end:+13383 MsG0080047991.01.T01:CDS
GTCTCTATAATAGCCTTGAT+GGG 0.560227 contig162end:-13710 None:intergenic
GGTGGCGGATGAAGTGTCAA+CGG 0.562818 contig162end:-13612 None:intergenic
TAACAACCAAGTTCCCATCA+AGG 0.571507 contig162end:+13697 MsG0080047991.01.T01:CDS
AAATGGTCCCAATGAGGCAT+CGG 0.576176 contig162end:+13292 MsG0080047991.01.T01:CDS
TCTCACCTTCCTTCCAACAA+TGG 0.578264 contig162end:+11282 MsG0080047991.01.T01:exon
TTGGAAAGCTTTGTCACAAG+AGG 0.581164 contig162end:+13876 MsG0080047991.01.T01:CDS
GTGCCTGCAGCCTTAATGGA+AGG 0.583698 contig162end:-13653 None:intergenic
GGCTCCAAGAGGACGACCAA+AGG 0.596610 contig162end:+11303 MsG0080047991.01.T01:CDS
CGCAGCTGTTTCAGTGTCTG+CGG 0.598889 contig162end:-13558 None:intergenic
ATTTGTGTTTAGGTTTATGA+CGG 0.600074 contig162end:+11604 MsG0080047991.01.T01:intron
AAGCCATGCATTCTAGACAC+AGG 0.600296 contig162end:-14126 None:intergenic
TCATCAACTTCCTATGACTG+TGG 0.601873 contig162end:+13433 MsG0080047991.01.T01:CDS
CTCGACGCTATGATTCCGTA+TGG 0.602511 contig162end:+11441 MsG0080047991.01.T01:CDS
ATAGTGCCAACAGCCTGACT+AGG 0.610418 contig162end:-13389 None:intergenic
AGAAGAGCAAGGTAGGAGAA+GGG 0.615751 contig162end:-13336 None:intergenic
AAGCTTTCCAATAGGTTGTG+AGG 0.617381 contig162end:-13864 None:intergenic
CCTGCAGCCTTAATGGAAGG+TGG 0.618279 contig162end:-13650 None:intergenic
TCATTTCACAGAAAGGTGAG+AGG 0.619282 contig162end:+11403 MsG0080047991.01.T01:intron
AGCCATGCATTCTAGACACA+GGG 0.619972 contig162end:-14125 None:intergenic
AAGAGGCGTCGACCTTGCCA+TGG 0.621523 contig162end:+13893 MsG0080047991.01.T01:CDS
TGGTTGGATTTCTGATGATG+AGG 0.621548 contig162end:+13913 MsG0080047991.01.T01:CDS
TCAGAAATCCAACCATGGCA+AGG 0.623253 contig162end:-13905 None:intergenic
GAGACTGTATCAGCAGACAA+TGG 0.637329 contig162end:+13728 MsG0080047991.01.T01:CDS
ATGTCCATGTTAGAAGAGCA+AGG 0.642571 contig162end:-13347 None:intergenic
CTATTCGTCAGACCATCGTA+TGG 0.644114 contig162end:+14161 MsG0080047991.01.T01:three_prime_UTR
TGGATTACTAGGTTGGCTGG+TGG 0.645107 contig162end:-13630 None:intergenic
TTGGATTTCTGATGATGAGG+AGG 0.658696 contig162end:+13916 MsG0080047991.01.T01:CDS
TCCAGGAAATGGTCCCAATG+AGG 0.658956 contig162end:+13286 MsG0080047991.01.T01:CDS
AGTTTGTTAGGAAAACCATA+CGG 0.668712 contig162end:-11456 None:intergenic
TTGATTACTTGGTTGACTGG+TGG 0.672311 contig162end:-13522 None:intergenic
ATTGTTGTTGAACCATACGA+TGG 0.676013 contig162end:-14173 None:intergenic
CTTCCAACAATGGCTCCAAG+AGG 0.677805 contig162end:+11292 MsG0080047991.01.T01:exon
CATCATCAGAAATCCAACCA+TGG 0.680406 contig162end:-13910 None:intergenic
ATTGTATAAGCTAGTGAATG+GGG 0.723488 contig162end:+13967 MsG0080047991.01.T01:CDS
GCCATGCATTCTAGACACAG+GGG 0.743557 contig162end:-14124 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGATCATATTATTAAATTAA+AGG + contig162end:11954-11973 MsG0080047991.01.T01:intron 10.0%
!! TTAAGAACAAAAATTAATAT+TGG + contig162end:12197-12216 MsG0080047991.01.T01:intron 10.0%
!! AAAAGATAAAGAAATTTCAT+TGG - contig162end:13006-13025 None:intergenic 15.0%
!! AGAAATGAAGAAAATAAAAA+TGG - contig162end:11365-11384 None:intergenic 15.0%
!! TACAATGAGAAATATATTTA+TGG + contig162end:12255-12274 MsG0080047991.01.T01:intron 15.0%
!! TCAATTATTGATATAATTTG+TGG + contig162end:11797-11816 MsG0080047991.01.T01:intron 15.0%
!! TTAATTAACTGTTCTATTAA+AGG - contig162end:12181-12200 None:intergenic 15.0%
!! TTCATTAAGTAGTAATTTAA+GGG - contig162end:12138-12157 None:intergenic 15.0%
!! TTTATAATTAAGAAACCAAA+AGG - contig162end:14233-14252 None:intergenic 15.0%
!! TTTCATTAAGTAGTAATTTA+AGG - contig162end:12139-12158 None:intergenic 15.0%
!!! AATTATTGATATAATTTGTG+GGG + contig162end:11799-11818 MsG0080047991.01.T01:intron 15.0%
!!! CAATTATTGATATAATTTGT+GGG + contig162end:11798-11817 MsG0080047991.01.T01:intron 15.0%
!!! TATAGAATTTTGACTAATAA+TGG + contig162end:13214-13233 MsG0080047991.01.T01:intron 15.0%
!! AATGAAGAAAATAAAAATGG+TGG - contig162end:11362-11381 None:intergenic 20.0%
!! ATTGAAACAAATACTGAATT+TGG - contig162end:12745-12764 None:intergenic 20.0%
!! CAAAATTCTTTATGATATAC+AGG - contig162end:13077-13096 None:intergenic 20.0%
!! TCAATCAAAATAACTAGTAT+TGG - contig162end:11842-11861 None:intergenic 20.0%
!!! CAGTATTTTGTTTATGTTAT+GGG + contig162end:12652-12671 MsG0080047991.01.T01:intron 20.0%
!!! GTTTTACTATATAAACCTTT+TGG + contig162end:14215-14234 MsG0080047991.01.T01:three_prime_UTR 20.0%
!!! TATCTTAAATTTGAGTTGTT+TGG - contig162end:12081-12100 None:intergenic 20.0%
!!! TCAGTATTTTGTTTATGTTA+TGG + contig162end:12651-12670 MsG0080047991.01.T01:intron 20.0%
!!! TTTCAATTTATGTTGATAGA+GGG + contig162end:12758-12777 MsG0080047991.01.T01:intron 20.0%
! AACTTGGTTGTTAATTTCAT+TGG - contig162end:13690-13709 None:intergenic 25.0%
! AAGTAATCAACTTTCCATTA+TGG + contig162end:13535-13554 MsG0080047991.01.T01:CDS 25.0%
! ATCTTACTTAGTTAAGTCTT+TGG + contig162end:14252-14271 MsG0080047991.01.T01:three_prime_UTR 25.0%
! ATGAAAAAAAAGCTAGTGAA+TGG + contig162end:13995-14014 MsG0080047991.01.T01:CDS 25.0%
! ATTGTTTATTATGCTTTGCT+TGG - contig162end:11741-11760 None:intergenic 25.0%
! CATTCATGTAATCGTTATTA+TGG - contig162end:14347-14366 None:intergenic 25.0%
! GAAAATGAAACGAAATTAGA+AGG - contig162end:11555-11574 None:intergenic 25.0%
! TATTTAACTACTCGTATAGT+AGG + contig162end:12504-12523 MsG0080047991.01.T01:intron 25.0%
! TTGTTTATTATGCTTTGCTT+GGG - contig162end:11740-11759 None:intergenic 25.0%
! TTTCATTTCATTTCACAGAA+AGG + contig162end:11396-11415 MsG0080047991.01.T01:intron 25.0%
!! AGTTGTTTGGTTCAATTATT+TGG - contig162end:12068-12087 None:intergenic 25.0%
!! ATAATGGAAAGTTGATTACT+TGG - contig162end:13536-13555 None:intergenic 25.0%
!! ATTTGTGTTTAGGTTTATGA+CGG + contig162end:11604-11623 MsG0080047991.01.T01:intron 25.0%
!! GTTTCAATTTATGTTGATAG+AGG + contig162end:12757-12776 MsG0080047991.01.T01:intron 25.0%
!! TTCAACAACAATACTTGTTT+TGG + contig162end:14184-14203 MsG0080047991.01.T01:three_prime_UTR 25.0%
!!! ACAATGCTTAATTTGTGTTT+AGG + contig162end:11594-11613 MsG0080047991.01.T01:intron 25.0%
!!! ATTCACTAGCTTTTTTTTCA+TGG - contig162end:13996-14015 None:intergenic 25.0%
!!! TTCACTAGCTTTTTTTTCAT+GGG - contig162end:13995-14014 None:intergenic 25.0%
AAGACATCTGTAACTTGAAA+AGG - contig162end:12400-12419 None:intergenic 30.0%
ACAAAATCCAATATGATCGA+AGG + contig162end:11908-11927 MsG0080047991.01.T01:intron 30.0%
ACAACAACAAAATCAGAATC+AGG + contig162end:11687-11706 MsG0080047991.01.T01:CDS 30.0%
AGACATCTGTAACTTGAAAA+GGG - contig162end:12399-12418 None:intergenic 30.0%
AGGATTGCAAATTAGCAAAT+TGG - contig162end:12543-12562 None:intergenic 30.0%
ATATACAGGCCATTAGATTA+AGG - contig162end:13063-13082 None:intergenic 30.0%
ATTGACAGATGTTAGTAGTT+AGG + contig162end:11857-11876 MsG0080047991.01.T01:intron 30.0%
ATTGTATAAGCTAGTGAATG+GGG + contig162end:13967-13986 MsG0080047991.01.T01:CDS 30.0%
ATTTGTAACTGTGTAAGAGA+TGG + contig162end:12294-12313 MsG0080047991.01.T01:intron 30.0%
CAAAATCCAATATGATCGAA+GGG + contig162end:11909-11928 MsG0080047991.01.T01:intron 30.0%
CATTGTATAAGCTAGTGAAT+GGG + contig162end:13966-13985 MsG0080047991.01.T01:CDS 30.0%
GAAATTAGAAGGTAATAACG+AGG - contig162end:11544-11563 None:intergenic 30.0%
GACTTATACCTCATGTATAA+TGG + contig162end:12340-12359 MsG0080047991.01.T01:intron 30.0%
GGATTGCAAATTAGCAAATT+GGG - contig162end:12542-12561 None:intergenic 30.0%
GTACGTTAATCATTACGATT+TGG + contig162end:12470-12489 MsG0080047991.01.T01:intron 30.0%
GTATATCTTGAATTCCTTCA+TGG + contig162end:12810-12829 MsG0080047991.01.T01:intron 30.0%
GTGAAGGATTATTTGATAGA+GGG + contig162end:13260-13279 MsG0080047991.01.T01:intron 30.0%
TAATCACACCATTATACATG+AGG - contig162end:12351-12370 None:intergenic 30.0%
TACTTTGAGCAATTGATCAA+CGG - contig162end:11493-11512 None:intergenic 30.0%
TGAAAAAGAAGAAACCATGA+AGG - contig162end:12827-12846 None:intergenic 30.0%
TGTGAAGGATTATTTGATAG+AGG + contig162end:13259-13278 MsG0080047991.01.T01:intron 30.0%
TGTTTATTATGCTTTGCTTG+GGG - contig162end:11739-11758 None:intergenic 30.0%
TTACTTAGTTAAGTCTTTGG+TGG + contig162end:14255-14274 MsG0080047991.01.T01:three_prime_UTR 30.0%
TTGACAGATGTTAGTAGTTA+GGG + contig162end:11858-11877 MsG0080047991.01.T01:intron 30.0%
TTTGGAGATGAGGTAAAATT+GGG + contig162end:12015-12034 MsG0080047991.01.T01:intron 30.0%
! AACAAATATTCCCTCACTTT+TGG + contig162end:11997-12016 MsG0080047991.01.T01:intron 30.0%
! ACATGTTGATTAACCATACT+AGG - contig162end:12912-12931 None:intergenic 30.0%
! GTTTGGTTCAATTATTTGGA+GGG - contig162end:12064-12083 None:intergenic 30.0%
! TAGTAATTTAAGGGTGTGTT+TGG - contig162end:12129-12148 None:intergenic 30.0%
! TGTTTGGTTCAATTATTTGG+AGG - contig162end:12065-12084 None:intergenic 30.0%
! TTTGGTTCAATTATTTGGAG+GGG - contig162end:12063-12082 None:intergenic 30.0%
!! AGTTTGTTAGGAAAACCATA+CGG - contig162end:11459-11478 None:intergenic 30.0%
!! TTTTGGAGATGAGGTAAAAT+TGG + contig162end:12014-12033 MsG0080047991.01.T01:intron 30.0%
!!! CTATTTTAACAATGCTTGAG+AGG + contig162end:12955-12974 MsG0080047991.01.T01:intron 30.0%
AGGTAATAACGAGGATTAAG+CGG - contig162end:11535-11554 None:intergenic 35.0%
AGTGTAACAACAGACAAAAG+AGG - contig162end:12563-12582 None:intergenic 35.0%
ATACGATACACAGACAAAAC+AGG - contig162end:13111-13130 None:intergenic 35.0%
ATACGATATACAGACAGAAC+AGG - contig162end:13197-13216 None:intergenic 35.0%
CTACTAGCTCCTTAATCTAA+TGG + contig162end:13051-13070 MsG0080047991.01.T01:intron 35.0%
GATGAATGTGAAAGAACCTT+TGG - contig162end:11322-11341 None:intergenic 35.0%
GCATTGTATAAGCTAGTGAA+TGG + contig162end:13965-13984 MsG0080047991.01.T01:CDS 35.0%
GTTTATTATGCTTTGCTTGG+GGG - contig162end:11738-11757 None:intergenic 35.0%
TAGTAATCCACCTTCCATTA+AGG + contig162end:13643-13662 MsG0080047991.01.T01:CDS 35.0%
TATTTGATAGAGGGTAATCC+AGG + contig162end:13269-13288 MsG0080047991.01.T01:CDS 35.0%
TCAAGTAGTGTTTAAAGAGC+AGG + contig162end:14020-14039 MsG0080047991.01.T01:exon 35.0%
TCTTGTATTGCTATGTGTGA+AGG + contig162end:13244-13263 MsG0080047991.01.T01:intron 35.0%
TGAGAACTGAGAATGTAGAA+AGG - contig162end:11267-11286 None:intergenic 35.0%
TTAGATTAAGGAGCTAGTAG+AGG - contig162end:13051-13070 None:intergenic 35.0%
TTGTGACAAAGCTTTCCAAT+AGG - contig162end:13875-13894 None:intergenic 35.0%
TTTGAGGAATTTCTGCAAGA+AGG - contig162end:13838-13857 None:intergenic 35.0%
! AAGTTGATTACTTGGTTGAC+TGG - contig162end:13528-13547 None:intergenic 35.0%
! AATTTAAGGGTGTGTTTGGT+TGG - contig162end:12125-12144 None:intergenic 35.0%
! AGTCTCTATAATAGCCTTGA+TGG - contig162end:13714-13733 None:intergenic 35.0%
! ATTGTTGTTGAACCATACGA+TGG - contig162end:14176-14195 None:intergenic 35.0%
! GTCTCTATAATAGCCTTGAT+GGG - contig162end:13713-13732 None:intergenic 35.0%
! TGTTGATTAACCATACTAGG+AGG - contig162end:12909-12928 None:intergenic 35.0%
! TTCACTAGCTTATACAATGC+AGG - contig162end:13965-13984 None:intergenic 35.0%
! TTTAGGTTTATGACGGTAGT+TGG + contig162end:11611-11630 MsG0080047991.01.T01:intron 35.0%
!! CATTACGATTTGGTTGTTGT+TGG + contig162end:12480-12499 MsG0080047991.01.T01:intron 35.0%
!! TTAATGGAAGGTGGATTACT+AGG - contig162end:13644-13663 None:intergenic 35.0%
AAGCTTTCCAATAGGTTGTG+AGG - contig162end:13867-13886 None:intergenic 40.0%
AATCATAACTTGAGCTGAGC+AGG - contig162end:12612-12631 None:intergenic 40.0%
AATTGACCGATGTTTGATGG+TGG + contig162end:12446-12465 MsG0080047991.01.T01:intron 40.0%
ACAAAGTCCTCACAACCTAT+TGG + contig162end:13857-13876 MsG0080047991.01.T01:CDS 40.0%
ACCTAAGCCTGAAACAATGT+AGG - contig162end:12701-12720 None:intergenic 40.0%
AGATGAGGTAAAATTGGGTC+AGG + contig162end:12020-12039 MsG0080047991.01.T01:intron 40.0%
ATAGAGGGTAATCCAGGAAA+TGG + contig162end:13275-13294 MsG0080047991.01.T01:CDS 40.0%
ATGTCCATGTTAGAAGAGCA+AGG - contig162end:13350-13369 None:intergenic 40.0%
CATCATCAGAAATCCAACCA+TGG - contig162end:13913-13932 None:intergenic 40.0%
CCAATGTCCTACATTGTTTC+AGG + contig162end:12691-12710 MsG0080047991.01.T01:intron 40.0%
CCTGAAACAATGTAGGACAT+TGG - contig162end:12694-12713 None:intergenic 40.0%
CTCCAAGGTTTCAGAATGAT+AGG + contig162end:14082-14101 MsG0080047991.01.T01:three_prime_UTR 40.0%
GATTAACCATACTAGGAGGT+TGG - contig162end:12905-12924 None:intergenic 40.0%
GCAAATTGACCGATGTTTGA+TGG + contig162end:12443-12462 MsG0080047991.01.T01:intron 40.0%
GTATCAGCAGACAATGGATA+TGG + contig162end:13734-13753 MsG0080047991.01.T01:CDS 40.0%
TAACAACCAAGTTCCCATCA+AGG + contig162end:13697-13716 MsG0080047991.01.T01:CDS 40.0%
TACCTCATCTCCAAAAGTGA+GGG - contig162end:12010-12029 None:intergenic 40.0%
TCATCAACTTCCTATGACTG+TGG + contig162end:13433-13452 MsG0080047991.01.T01:CDS 40.0%
TCATTTCACAGAAAGGTGAG+AGG + contig162end:11403-11422 MsG0080047991.01.T01:intron 40.0%
TCCTACATTGTTTCAGGCTT+AGG + contig162end:12697-12716 MsG0080047991.01.T01:intron 40.0%
TGTGTTTGGTTGGAGAGAAA+TGG - contig162end:12115-12134 None:intergenic 40.0%
TTACCTCATCTCCAAAAGTG+AGG - contig162end:12011-12030 None:intergenic 40.0%
TTGATTACTTGGTTGACTGG+TGG - contig162end:13525-13544 None:intergenic 40.0%
TTTGGTTGGAGAGAAATGGA+GGG - contig162end:12111-12130 None:intergenic 40.0%
! GCTAGTAGAGGATTTTCATG+AGG - contig162end:13039-13058 None:intergenic 40.0%
! GTTGTATCAGATTAGCTCCA+AGG + contig162end:14067-14086 MsG0080047991.01.T01:three_prime_UTR 40.0%
! GTTTGACGAACGAGTTTGTT+AGG - contig162end:11471-11490 None:intergenic 40.0%
! TAATAGCCTTGATGGGAACT+TGG - contig162end:13706-13725 None:intergenic 40.0%
! TGGTTGGATTTCTGATGATG+AGG + contig162end:13913-13932 MsG0080047991.01.T01:CDS 40.0%
! TTGGAAAGCTTTGTCACAAG+AGG + contig162end:13876-13895 MsG0080047991.01.T01:CDS 40.0%
! TTGGATTTCTGATGATGAGG+AGG + contig162end:13916-13935 MsG0080047991.01.T01:CDS 40.0%
!! AAGTTGATGACCAGTTTCAC+TGG - contig162end:13423-13442 None:intergenic 40.0%
!! CACCTATCATTCTGAAACCT+TGG - contig162end:14087-14106 None:intergenic 40.0%
AAATGGTCCCAATGAGGCAT+CGG + contig162end:13292-13311 MsG0080047991.01.T01:CDS 45.0%
AAGCCATGCATTCTAGACAC+AGG - contig162end:14129-14148 None:intergenic 45.0%
ACAGAAAGGTGAGAGGAGAA+TGG + contig162end:11410-11429 MsG0080047991.01.T01:intron 45.0%
AGAAGAGCAAGGTAGGAGAA+GGG - contig162end:13339-13358 None:intergenic 45.0%
AGCCATGCATTCTAGACACA+GGG - contig162end:14128-14147 None:intergenic 45.0%
AGCTGAGCAGGACATAAAAC+TGG - contig162end:12600-12619 None:intergenic 45.0%
ATATCAGCAGCCAGTGAAAC+TGG + contig162end:13410-13429 MsG0080047991.01.T01:CDS 45.0%
ATTACTTGGTTGACTGGTGG+CGG - contig162end:13522-13541 None:intergenic 45.0%
CAATATGATCGAAGGGAGAG+GGG + contig162end:11916-11935 MsG0080047991.01.T01:intron 45.0%
CATTGTTTCAGGCTTAGGTC+TGG + contig162end:12702-12721 MsG0080047991.01.T01:intron 45.0%
CCAATATGATCGAAGGGAGA+GGG + contig162end:11915-11934 MsG0080047991.01.T01:intron 45.0%
CCATGTTAGAAGAGCAAGGT+AGG - contig162end:13346-13365 None:intergenic 45.0%
CCTACCTTGCTCTTCTAACA+TGG + contig162end:13343-13362 MsG0080047991.01.T01:CDS 45.0%
CGTACACCACCATCAAACAT+CGG - contig162end:12455-12474 None:intergenic 45.0%
CGTCCCATAGAGTCAATAAC+GGG + contig162end:12317-12336 MsG0080047991.01.T01:intron 45.0%
CTATTCGTCAGACCATCGTA+TGG + contig162end:14161-14180 MsG0080047991.01.T01:three_prime_UTR 45.0%
GAGACTGTATCAGCAGACAA+TGG + contig162end:13728-13747 MsG0080047991.01.T01:CDS 45.0%
GAGGTAAAATTGGGTCAGGA+GGG + contig162end:12024-12043 MsG0080047991.01.T01:intron 45.0%
GCGTCCCATAGAGTCAATAA+CGG + contig162end:12316-12335 MsG0080047991.01.T01:intron 45.0%
GCTGAGCAGGACATAAAACT+GGG - contig162end:12599-12618 None:intergenic 45.0%
GTCCCATAGAGTCAATAACG+GGG + contig162end:12318-12337 MsG0080047991.01.T01:intron 45.0%
GTTTGGTTGGAGAGAAATGG+AGG - contig162end:12112-12131 None:intergenic 45.0%
TAGAAGAGCAAGGTAGGAGA+AGG - contig162end:13340-13359 None:intergenic 45.0%
TCAGAAATCCAACCATGGCA+AGG - contig162end:13908-13927 None:intergenic 45.0%
TCCAATATGATCGAAGGGAG+AGG + contig162end:11914-11933 MsG0080047991.01.T01:intron 45.0%
TCTCACCTTCCTTCCAACAA+TGG + contig162end:11282-11301 MsG0080047991.01.T01:exon 45.0%
TGAGGTAAAATTGGGTCAGG+AGG + contig162end:12023-12042 MsG0080047991.01.T01:intron 45.0%
TTAGCTCCAACCTCCTAGTA+TGG + contig162end:12896-12915 MsG0080047991.01.T01:intron 45.0%
TTGGTTGGAGAGAAATGGAG+GGG - contig162end:12110-12129 None:intergenic 45.0%
! ACTAGGTAATGGCGCATCAT+CGG - contig162end:13375-13394 None:intergenic 45.0%
! GATTTCTGATGATGAGGAGG+AGG + contig162end:13919-13938 MsG0080047991.01.T01:CDS 45.0%
! GGTTGTGAGGACTTTGTTTG+AGG - contig162end:13854-13873 None:intergenic 45.0%
!! AGTCCCCGTTATTGACTCTA+TGG - contig162end:12324-12343 None:intergenic 45.0%
!! GAATTGTTGACGGGCTTGAT+GGG - contig162end:13497-13516 None:intergenic 45.0%
!! GTCCCCGTTATTGACTCTAT+GGG - contig162end:12323-12342 None:intergenic 45.0%
!! TGAATTGTTGACGGGCTTGA+TGG - contig162end:13498-13517 None:intergenic 45.0%
!! TGGAAGGTGGATTACTAGGT+TGG - contig162end:13640-13659 None:intergenic 45.0%
!! TTCCCTCACTTTTGGAGATG+AGG + contig162end:12005-12024 MsG0080047991.01.T01:intron 45.0%
!! TTAAATAAAATTAATTAACA+AGG - contig162end:13775-13794 None:intergenic 5.0%
AGAACCTTTGGTCGTCCTCT+TGG - contig162end:11310-11329 None:intergenic 50.0%
ATAGTGCCAACAGCCTGACT+AGG - contig162end:13392-13411 None:intergenic 50.0%
CCAACAGCCTGACTAGGTAA+TGG - contig162end:13386-13405 None:intergenic 50.0%
CCATTACCTAGTCAGGCTGT+TGG + contig162end:13383-13402 MsG0080047991.01.T01:CDS 50.0%
CCCTCTCCCTTCGATCATAT+TGG - contig162end:11918-11937 None:intergenic 50.0%
CTCGACGCTATGATTCCGTA+TGG + contig162end:11441-11460 MsG0080047991.01.T01:CDS 50.0%
CTCTTGGAGCCATTGTTGGA+AGG - contig162end:11294-11313 None:intergenic 50.0%
CTTCCAACAATGGCTCCAAG+AGG + contig162end:11292-11311 MsG0080047991.01.T01:exon 50.0%
GATGCAGTGTCCACAGTCAT+AGG - contig162end:13446-13465 None:intergenic 50.0%
GCCATGCATTCTAGACACAG+GGG - contig162end:14127-14146 None:intergenic 50.0%
TACAGTGCCTGCAGCCTTAA+TGG - contig162end:13660-13679 None:intergenic 50.0%
TCCAGGAAATGGTCCCAATG+AGG + contig162end:13286-13305 MsG0080047991.01.T01:CDS 50.0%
TGATGCGCCATTACCTAGTC+AGG + contig162end:13376-13395 MsG0080047991.01.T01:CDS 50.0%
TGGAGCCATTGTTGGAAGGA+AGG - contig162end:11290-11309 None:intergenic 50.0%
TTCAGTGTCTGCGGCCATAA+TGG - contig162end:13552-13571 None:intergenic 50.0%
! ATTACTAGGTTGGCTGGTGG+CGG - contig162end:13630-13649 None:intergenic 50.0%
! GAAGTGTCAACGGGCTTGAT+GGG - contig162end:13605-13624 None:intergenic 50.0%
! TCCCCTGTGTCTAGAATGCA+TGG + contig162end:14123-14142 MsG0080047991.01.T01:three_prime_UTR 50.0%
! TCTGATGATGAGGAGGAGGA+GGG + contig162end:13923-13942 MsG0080047991.01.T01:CDS 50.0%
! TGAAGTGTCAACGGGCTTGA+TGG - contig162end:13606-13625 None:intergenic 50.0%
! TGGATTACTAGGTTGGCTGG+TGG - contig162end:13633-13652 None:intergenic 50.0%
! TTCTGATGATGAGGAGGAGG+AGG + contig162end:13922-13941 MsG0080047991.01.T01:CDS 50.0%
!! AGGTGGATTACTAGGTTGGC+TGG - contig162end:13636-13655 None:intergenic 50.0%
ACCAGAAGCCGATGCCTCAT+TGG - contig162end:13303-13322 None:intergenic 55.0%
CCACCTTCCATTAAGGCTGC+AGG + contig162end:13650-13669 MsG0080047991.01.T01:CDS 55.0%
CCAGAAGCCGATGCCTCATT+GGG - contig162end:13302-13321 None:intergenic 55.0%
CCTGCAGCCTTAATGGAAGG+TGG - contig162end:13653-13672 None:intergenic 55.0%
CGTCCTCTTGGAGCCATTGT+TGG - contig162end:11298-11317 None:intergenic 55.0%
GCCTCATTGGGACCATTTCC+TGG - contig162end:13290-13309 None:intergenic 55.0%
GTGCCTGCAGCCTTAATGGA+AGG - contig162end:13656-13675 None:intergenic 55.0%
GTGGCGGCTGAATTGTTGAC+GGG - contig162end:13506-13525 None:intergenic 55.0%
TATGATCGAAGGGAGAGGGG+AGG + contig162end:11919-11938 MsG0080047991.01.T01:intron 55.0%
! CGCAGCTGTTTCAGTGTCTG+CGG - contig162end:13561-13580 None:intergenic 55.0%
! CTGATGATGAGGAGGAGGAG+GGG + contig162end:13924-13943 MsG0080047991.01.T01:CDS 55.0%
! GGTGGCGGCTGAATTGTTGA+CGG - contig162end:13507-13526 None:intergenic 55.0%
! TGATGATGAGGAGGAGGAGG+GGG + contig162end:13925-13944 MsG0080047991.01.T01:CDS 55.0%
!! GGTGGCGGATGAAGTGTCAA+CGG - contig162end:13615-13634 None:intergenic 55.0%
!! GTGGCGGATGAAGTGTCAAC+GGG - contig162end:13614-13633 None:intergenic 55.0%
GGCTCCAAGAGGACGACCAA+AGG + contig162end:11303-11322 MsG0080047991.01.T01:CDS 60.0%
!! AAGAGGCGTCGACCTTGCCA+TGG + contig162end:13893-13912 MsG0080047991.01.T01:CDS 60.0%
!! CCCAATGAGGCATCGGCTTC+TGG + contig162end:13299-13318 MsG0080047991.01.T01:CDS 60.0%
CGAAGGGAGAGGGGAGGAGA+GGG + contig162end:11925-11944 MsG0080047991.01.T01:intron 65.0%
TCGAAGGGAGAGGGGAGGAG+AGG + contig162end:11924-11943 MsG0080047991.01.T01:intron 65.0%
!! GGCGTCGACCTTGCCATGGT+TGG + contig162end:13897-13916 MsG0080047991.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
contig162end gene 11257 14368 11257 ID=MsG0080047991.01;Name=MsG0080047991.01
contig162end mRNA 11257 14368 11257 ID=MsG0080047991.01.T01;Parent=MsG0080047991.01;Name=MsG0080047991.01.T01;_AED=0.45;_eAED=0.45;_QI=44|1|1|1|0.75|0.6|5|341|294
contig162end exon 11257 11324 11257 ID=MsG0080047991.01.T01:exon:8202;Parent=MsG0080047991.01.T01
contig162end exon 11414 11509 11414 ID=MsG0080047991.01.T01:exon:8203;Parent=MsG0080047991.01.T01
contig162end exon 11616 11708 11616 ID=MsG0080047991.01.T01:exon:8204;Parent=MsG0080047991.01.T01
contig162end exon 13266 13755 13266 ID=MsG0080047991.01.T01:exon:8205;Parent=MsG0080047991.01.T01
contig162end exon 13846 14368 13846 ID=MsG0080047991.01.T01:exon:8206;Parent=MsG0080047991.01.T01
contig162end five_prime_UTR 11257 11300 11257 ID=MsG0080047991.01.T01:five_prime_utr;Parent=MsG0080047991.01.T01
contig162end CDS 11301 11324 11301 ID=MsG0080047991.01.T01:cds;Parent=MsG0080047991.01.T01
contig162end CDS 11414 11509 11414 ID=MsG0080047991.01.T01:cds;Parent=MsG0080047991.01.T01
contig162end CDS 11616 11708 11616 ID=MsG0080047991.01.T01:cds;Parent=MsG0080047991.01.T01
contig162end CDS 13266 13755 13266 ID=MsG0080047991.01.T01:cds;Parent=MsG0080047991.01.T01
contig162end CDS 13846 14027 13846 ID=MsG0080047991.01.T01:cds;Parent=MsG0080047991.01.T01
contig162end three_prime_UTR 14028 14368 14028 ID=MsG0080047991.01.T01:three_prime_utr;Parent=MsG0080047991.01.T01
Gene Sequence

>MsG0080047991.01.T01

ATGGCTCCAAGAGGACGACCAAAGAAAGGTGAGAGGAGAATGGACGCAGCTCTCGACGCTATGATTCCGTATGGTTTTCCTAACAAACTCGTTCGTCAAACCGTTGATCAATTGCTCAAAGTTTATGACGGTAGTTGGTTTTTCATTGAAGAATCTGCTTATACTCTTCTCATTGATACTATTCTTGAAAAACAACAACAAAATCAGAATCAGGATTATTTGATAGAGGGTAATCCAGGAAATGGTCCCAATGAGGCATCGGCTTCTGGTTGCTCAAACAGAACCCTTCTCCTACCTTGCTCTTCTAACATGGACATGTCCGATGATGCGCCATTACCTAGTCAGGCTGTTGGCACTATATCAGCAGCCAGTGAAACTGGTCATCAACTTCCTATGACTGTGGACACTGCATCAGCTACAAGCAAAGCTGTTATTGAGCTTCCCATCAAGCCCGTCAACAATTCAGCCGCCACCAGTCAACCAAGTAATCAACTTTCCATTATGGCCGCAGACACTGAAACAGCTGCGAGCAAAACTGTTAATGAACGTCCCATCAAGCCCGTTGACACTTCATCCGCCACCAGCCAACCTAGTAATCCACCTTCCATTAAGGCTGCAGGCACTGTATCTGCAACCAATGAAATTAACAACCAAGTTCCCATCAAGGCTATTATAGAGACTGTATCAGCAGACAATGGATATGAAATTCCTCAAACAAAGTCCTCACAACCTATTGGAAAGCTTTGTCACAAGAGGCGTCGACCTTGCCATGGTTGGATTTCTGATGATGAGGAGGAGGAGGGGGAGTTAATAGAACTACCTGCATTGTATAAGCTAGTGAATGGGGTGCCCATGAAAAAAAAGCTAGTGAATGGAGTCAAGTAG
Protein sequence

>MsG0080047991.01.T01

MAPRGRPKKGERRMDAALDAMIPYGFPNKLVRQTVDQLLKVYDGSWFFIEESAYTLLIDTILEKQQQNQNQDYLIEGNPGNGPNEASASGCSNRTLLLPCSSNMDMSDDAPLPSQAVGTISAASETGHQLPMTVDTASATSKAVIELPIKPVNNSAATSQPSNQLSIMAADTETAASKTVNERPIKPVDTSSATSQPSNPPSIKAAGTVSATNEINNQVPIKAIIETVSADNGYEIPQTKSSQPIGKLCHKRRRPCHGWISDDEEEEGELIELPALYKLVNGVPMKKKLVNGVK*