AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0780039838.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 29 sgRNAs with CRISPR-Local

Find 29 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACACTCTTGGAATTCAAAA+TGG 0.249767 7:-69602663 MsG0780039838.01.T01:CDS
ATCTCTGCACCTCCCCTAAA+TGG 0.258474 7:+69602558 None:intergenic
ACGGTGGTGCCAATCCATTT+AGG 0.358436 7:-69602572 MsG0780039838.01.T01:CDS
CGGTGGTGCCAATCCATTTA+GGG 0.375482 7:-69602571 MsG0780039838.01.T01:CDS
GCTCTTGAGATTAACACTCT+TGG 0.426875 7:-69602675 MsG0780039838.01.T01:CDS
GCATGTGGAGAGTGAAGAAT+TGG 0.443078 7:-69602517 MsG0780039838.01.T01:CDS
AGATTAAGAGCCATTGGTGT+TGG 0.490679 7:-69602539 MsG0780039838.01.T01:CDS
TTTCTGTGTAAGATCAAGCT+TGG 0.505371 7:+69602721 None:intergenic
ACTGGCCCCACCGTGACGGT+TGG 0.511801 7:+69602749 None:intergenic
AGATCAAGCTTGGAAACTAC+TGG 0.514531 7:+69602731 None:intergenic
AGCTTAAGCTTGAGGAAGAG+TGG 0.529887 7:+69602695 None:intergenic
ATCTCAAGAGCTTAAGCTTG+AGG 0.533549 7:+69602687 None:intergenic
TGCACCTCCCCTAAATGGAT+TGG 0.536322 7:+69602563 None:intergenic
GTGCAGAGATTAAGAGCCAT+TGG 0.539401 7:-69602545 MsG0780039838.01.T01:CDS
GCTTAAGCTTGAGGAAGAGT+GGG 0.565601 7:+69602696 None:intergenic
AACTACTGGCCCCACCGTGA+CGG 0.577342 7:+69602745 None:intergenic
AAGTTAACAAGGATACGTTC+TGG 0.581922 7:+69602783 None:intergenic
TCTCCACATGCCAACACCAA+TGG 0.584573 7:+69602529 None:intergenic
TCTTCATCGAAGCTATCACT+TGG 0.591272 7:+69602608 None:intergenic
GAGCCATTGGTGTTGGCATG+TGG 0.594989 7:-69602532 MsG0780039838.01.T01:CDS
ACTTGTCCAACCGTCACGGT+GGG 0.599149 7:-69602755 MsG0780039838.01.T01:CDS
AACTTGTCCAACCGTCACGG+TGG 0.625736 7:-69602756 MsG0780039838.01.T01:CDS
CTTGTCCAACCGTCACGGTG+GGG 0.634010 7:-69602754 MsG0780039838.01.T01:CDS
GGTGGTGCCAATCCATTTAG+GGG 0.641245 7:-69602570 MsG0780039838.01.T01:CDS
ACAAGTTACTGAAGTTAACA+AGG 0.643513 7:+69602772 None:intergenic
TGAAGAAGTACTATCGAAGA+CGG 0.654052 7:-69602591 MsG0780039838.01.T01:CDS
AGTAACTTGTCCAACCGTCA+CGG 0.658820 7:-69602759 MsG0780039838.01.T01:CDS
GGTGCCAATCCATTTAGGGG+AGG 0.674114 7:-69602567 MsG0780039838.01.T01:CDS
AGAAGTACTATCGAAGACGG+TGG 0.755717 7:-69602588 MsG0780039838.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
AACACTCTTGGAATTCAAAA+TGG - Chr7:69602633-69602652 MsG0780039838.01.T01:CDS 30.0%
ACAAGTTACTGAAGTTAACA+AGG + Chr7:69602527-69602546 None:intergenic 30.0%
AAGTTAACAAGGATACGTTC+TGG + Chr7:69602516-69602535 None:intergenic 35.0%
TTTCTGTGTAAGATCAAGCT+TGG + Chr7:69602578-69602597 None:intergenic 35.0%
!! TGAAGAAGTACTATCGAAGA+CGG - Chr7:69602705-69602724 MsG0780039838.01.T01:CDS 35.0%
AGATCAAGCTTGGAAACTAC+TGG + Chr7:69602568-69602587 None:intergenic 40.0%
AGATTAAGAGCCATTGGTGT+TGG - Chr7:69602757-69602776 MsG0780039838.01.T01:CDS 40.0%
ATCTCAAGAGCTTAAGCTTG+AGG + Chr7:69602612-69602631 None:intergenic 40.0%
GCTCTTGAGATTAACACTCT+TGG - Chr7:69602621-69602640 MsG0780039838.01.T01:CDS 40.0%
TCTTCATCGAAGCTATCACT+TGG + Chr7:69602691-69602710 None:intergenic 40.0%
AGCTTAAGCTTGAGGAAGAG+TGG + Chr7:69602604-69602623 None:intergenic 45.0%
GCATGTGGAGAGTGAAGAAT+TGG - Chr7:69602779-69602798 MsG0780039838.01.T01:CDS 45.0%
GCTTAAGCTTGAGGAAGAGT+GGG + Chr7:69602603-69602622 None:intergenic 45.0%
GTGCAGAGATTAAGAGCCAT+TGG - Chr7:69602751-69602770 MsG0780039838.01.T01:CDS 45.0%
! AGTAACTTGTCCAACCGTCA+CGG - Chr7:69602537-69602556 MsG0780039838.01.T01:CDS 45.0%
!! AGAAGTACTATCGAAGACGG+TGG - Chr7:69602708-69602727 MsG0780039838.01.T01:CDS 45.0%
ATCTCTGCACCTCCCCTAAA+TGG + Chr7:69602741-69602760 None:intergenic 50.0%
TCTCCACATGCCAACACCAA+TGG + Chr7:69602770-69602789 None:intergenic 50.0%
TGCACCTCCCCTAAATGGAT+TGG + Chr7:69602736-69602755 None:intergenic 50.0%
! ACGGTGGTGCCAATCCATTT+AGG - Chr7:69602724-69602743 MsG0780039838.01.T01:CDS 50.0%
! CGGTGGTGCCAATCCATTTA+GGG - Chr7:69602725-69602744 MsG0780039838.01.T01:CDS 50.0%
! GGTGGTGCCAATCCATTTAG+GGG - Chr7:69602726-69602745 MsG0780039838.01.T01:CDS 50.0%
AACTTGTCCAACCGTCACGG+TGG - Chr7:69602540-69602559 MsG0780039838.01.T01:CDS 55.0%
ACTTGTCCAACCGTCACGGT+GGG - Chr7:69602541-69602560 MsG0780039838.01.T01:CDS 55.0%
GGTGCCAATCCATTTAGGGG+AGG - Chr7:69602729-69602748 MsG0780039838.01.T01:CDS 55.0%
!! GAGCCATTGGTGTTGGCATG+TGG - Chr7:69602764-69602783 MsG0780039838.01.T01:CDS 55.0%
AACTACTGGCCCCACCGTGA+CGG + Chr7:69602554-69602573 None:intergenic 60.0%
CTTGTCCAACCGTCACGGTG+GGG - Chr7:69602542-69602561 MsG0780039838.01.T01:CDS 60.0%
ACTGGCCCCACCGTGACGGT+TGG + Chr7:69602550-69602569 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
Chr7 gene 69602508 69602810 69602508 ID=MsG0780039838.01;Name=MsG0780039838.01
Chr7 mRNA 69602508 69602810 69602508 ID=MsG0780039838.01.T01;Parent=MsG0780039838.01;Name=MsG0780039838.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|100
Chr7 exon 69602508 69602810 69602508 ID=MsG0780039838.01.T01:exon:12639;Parent=MsG0780039838.01.T01
Chr7 CDS 69602508 69602810 69602508 ID=MsG0780039838.01.T01:cds;Parent=MsG0780039838.01.T01
Gene Sequence

>MsG0780039838.01.T01

ATGGGCCAGAACGTATCCTTGTTAACTTCAGTAACTTGTCCAACCGTCACGGTGGGGCCAGTAGTTTCCAAGCTTGATCTTACACAGAAACTCCCACTCTTCCTCAAGCTTAAGCTCTTGAGATTAACACTCTTGGAATTCAAAATGGAAGAAGAAAATGAAGTAGTAGAAGAAGAAGAACCAAGTGATAGCTTCGATGAAGAAGTACTATCGAAGACGGTGGTGCCAATCCATTTAGGGGAGGTGCAGAGATTAAGAGCCATTGGTGTTGGCATGTGGAGAGTGAAGAATTGGACTCTCTGA

Protein sequence

>MsG0780039838.01.T01

MGQNVSLLTSVTCPTVTVGPVVSKLDLTQKLPLFLKLKLLRLTLLEFKMEEENEVVEEEEPSDSFDEEVLSKTVVPIHLGEVQRLRAIGVGMWRVKNWTL*