Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048009.01.T01 | KAG5128813.1 | 97.248 | 109 | 3 | 0 | 5 | 113 | 90 | 198 | 7.02E-67 | 210 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048009.01.T01 | Q04614 | 88.889 | 90 | 10 | 0 | 1 | 90 | 11 | 100 | 1.61E-46 | 147 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048009.01.T01 | A0A6A5LBZ9 | 96.429 | 112 | 4 | 0 | 2 | 113 | 83 | 194 | 1.41e-66 | 209 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048008.01 | MsG0080048009.01 | 0.840368 | 9.070175e-58 | 1.289214e-54 |
| MsG0080048009.01 | MsG0080048010.01 | 0.842719 | 2.178370e-58 | 3.331592e-55 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048009.01.T01 | ATMG00650-2 | 97.778 | 90 | 2 | 0 | 1 | 90 | 11 | 100 | 6.15e-55 | 166 |
Find 28 sgRNAs with CRISPR-Local
Find 38 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAGGAATATTTCGTCTATTA+AGG | 0.231393 | contig169end:+13663 | None:intergenic |
| TCAATTGGCATTGACATAAT+AGG | 0.238962 | contig169end:+13644 | None:intergenic |
| GGGTCAATCATTTGCTTCAT+TGG | 0.294167 | contig169end:-13558 | MsG0080048009.01.T01:CDS |
| AGGGTCGGGGCCTTTCTCGC+TGG | 0.295326 | contig169end:-13339 | MsG0080048009.01.T01:CDS |
| TCGGAAAGTTATAACGAAAA+TGG | 0.328466 | contig169end:+13496 | None:intergenic |
| CGTTTCTTCGGATGATATGA+TGG | 0.347781 | contig169end:-13579 | MsG0080048009.01.T01:CDS |
| AACTAAACGGGAAGTGGTTT+AGG | 0.356151 | contig169end:+13284 | None:intergenic |
| GCAGCTGCAGAATCAGCTAT+TGG | 0.377057 | contig169end:-13524 | MsG0080048009.01.T01:CDS |
| GCTAATAACATTGATTCAAT+TGG | 0.381758 | contig169end:+13629 | None:intergenic |
| GAATTTATTAATAGCATTCA+AGG | 0.400684 | contig169end:-13452 | MsG0080048009.01.T01:intron |
| GGGTCGGGGCCTTTCTCGCT+GGG | 0.406902 | contig169end:-13338 | MsG0080048009.01.T01:CDS |
| TCTATCTTTCAGCAACTAAA+CGG | 0.410381 | contig169end:+13271 | None:intergenic |
| CAGCTGCAGAATCAGCTATT+GGG | 0.433456 | contig169end:-13523 | MsG0080048009.01.T01:CDS |
| ATGCGCTCGCCCAGCGAGAA+AGG | 0.450622 | contig169end:+13329 | None:intergenic |
| GAAAGGACTTTAGAATCAAT+CGG | 0.458968 | contig169end:+13306 | None:intergenic |
| CTATCTTTCAGCAACTAAAC+GGG | 0.463806 | contig169end:+13272 | None:intergenic |
| TTACTTGGTTGGCAGGGTCG+GGG | 0.477610 | contig169end:-13352 | MsG0080048009.01.T01:intron |
| TTCAGCAACTAAACGGGAAG+TGG | 0.479268 | contig169end:+13278 | None:intergenic |
| CAGCAATAGTCCCTCGGACT+CGG | 0.481853 | contig169end:+13477 | None:intergenic |
| CATCATATCATCCGAAGAAA+CGG | 0.510329 | contig169end:+13580 | None:intergenic |
| GTTTCTTCGGATGATATGAT+GGG | 0.537748 | contig169end:-13578 | MsG0080048009.01.T01:CDS |
| ATTTGCTTCATTGGTTTCAA+CGG | 0.542574 | contig169end:-13549 | MsG0080048009.01.T01:CDS |
| TTATAACTTTCCGAGTCCGA+GGG | 0.549621 | contig169end:-13487 | MsG0080048009.01.T01:CDS |
| TGCTTCATTGGTTTCAACGG+TGG | 0.570512 | contig169end:-13546 | MsG0080048009.01.T01:CDS |
| GTTATAACTTTCCGAGTCCG+AGG | 0.586018 | contig169end:-13488 | MsG0080048009.01.T01:CDS |
| GAATATTTCGTCTATTAAGG+AGG | 0.586482 | contig169end:+13666 | None:intergenic |
| ATTCTACAGCAATAGTCCCT+CGG | 0.611872 | contig169end:+13471 | None:intergenic |
| AACGGGAAGTGGTTTAGGAA+AGG | 0.614295 | contig169end:+13289 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | CTTTTGTTTTTATTTTTACT+TGG | - | contig169end:13617-13636 | MsG0080048009.01.T01:CDS | 15.0% |
| !! | GAATTTATTAATAGCATTCA+AGG | - | contig169end:13532-13551 | MsG0080048009.01.T01:CDS | 20.0% |
| !!! | TGTTTTTATTTTTACTTGGT+TGG | - | contig169end:13621-13640 | MsG0080048009.01.T01:CDS | 20.0% |
| ! | TAGGAATATTTCGTCTATTA+AGG | + | contig169end:13324-13343 | None:intergenic | 25.0% |
| !! | GAAAAGATAACTTCGTATTT+TGG | + | contig169end:13582-13601 | None:intergenic | 25.0% |
| !!! | GCTAATAACATTGATTCAAT+TGG | + | contig169end:13358-13377 | None:intergenic | 25.0% |
| !!! | TCATCTCTATTTTAGGTATT+CGG | - | contig169end:13290-13309 | MsG0080048009.01.T01:CDS | 25.0% |
| AAACAAAAGAAGAGAACGAA+AGG | + | contig169end:13606-13625 | None:intergenic | 30.0% | |
| GAATATTTCGTCTATTAAGG+AGG | + | contig169end:13321-13340 | None:intergenic | 30.0% | |
| TCGGAAAGTTATAACGAAAA+TGG | + | contig169end:13491-13510 | None:intergenic | 30.0% | |
| ! | TTTGGTATTTTCCGTTTCTT+CGG | - | contig169end:13393-13412 | MsG0080048009.01.T01:intron | 30.0% |
| !! | ATCTCTATTTTAGGTATTCG+GGG | - | contig169end:13292-13311 | MsG0080048009.01.T01:CDS | 30.0% |
| !! | ATTTGCTTCATTGGTTTCAA+CGG | - | contig169end:13435-13454 | MsG0080048009.01.T01:intron | 30.0% |
| !! | CATCTCTATTTTAGGTATTC+GGG | - | contig169end:13291-13310 | MsG0080048009.01.T01:CDS | 30.0% |
| !! | GAAAGGACTTTAGAATCAAT+CGG | + | contig169end:13681-13700 | None:intergenic | 30.0% |
| !! | TCAATTGGCATTGACATAAT+AGG | + | contig169end:13343-13362 | None:intergenic | 30.0% |
| !!! | TCGTATTTTGGTAATTCTCT+AGG | + | contig169end:13570-13589 | None:intergenic | 30.0% |
| !!! | TTATTTTTACTTGGTTGGCA+GGG | - | contig169end:13626-13645 | MsG0080048009.01.T01:CDS | 30.0% |
| !!! | TTTATTTTTACTTGGTTGGC+AGG | - | contig169end:13625-13644 | MsG0080048009.01.T01:CDS | 30.0% |
| CATCATATCATCCGAAGAAA+CGG | + | contig169end:13407-13426 | None:intergenic | 35.0% | |
| GTTTCTTCGGATGATATGAT+GGG | - | contig169end:13406-13425 | MsG0080048009.01.T01:intron | 35.0% | |
| ! | AGCTGTGAATTCGAACTTTT+TGG | - | contig169end:13375-13394 | MsG0080048009.01.T01:intron | 35.0% |
| ATTCTACAGCAATAGTCCCT+CGG | + | contig169end:13516-13535 | None:intergenic | 40.0% | |
| CGTTTCTTCGGATGATATGA+TGG | - | contig169end:13405-13424 | MsG0080048009.01.T01:intron | 40.0% | |
| GGGTCAATCATTTGCTTCAT+TGG | - | contig169end:13426-13445 | MsG0080048009.01.T01:intron | 40.0% | |
| TTATAACTTTCCGAGTCCGA+GGG | - | contig169end:13497-13516 | MsG0080048009.01.T01:CDS | 40.0% | |
| AACGGGAAGTGGTTTAGGAA+AGG | + | contig169end:13698-13717 | None:intergenic | 45.0% | |
| CAGCTGCAGAATCAGCTATT+GGG | - | contig169end:13461-13480 | MsG0080048009.01.T01:CDS | 45.0% | |
| GTTATAACTTTCCGAGTCCG+AGG | - | contig169end:13496-13515 | MsG0080048009.01.T01:CDS | 45.0% | |
| ! | TTTTACTTGGTTGGCAGGGT+CGG | - | contig169end:13630-13649 | MsG0080048009.01.T01:CDS | 45.0% |
| !! | TGCTTCATTGGTTTCAACGG+TGG | - | contig169end:13438-13457 | MsG0080048009.01.T01:intron | 45.0% |
| GCAGCTGCAGAATCAGCTAT+TGG | - | contig169end:13460-13479 | MsG0080048009.01.T01:CDS | 50.0% | |
| TTTACTTGGTTGGCAGGGTC+GGG | - | contig169end:13631-13650 | MsG0080048009.01.T01:CDS | 50.0% | |
| CAGCAATAGTCCCTCGGACT+CGG | + | contig169end:13510-13529 | None:intergenic | 55.0% | |
| TTACTTGGTTGGCAGGGTCG+GGG | - | contig169end:13632-13651 | MsG0080048009.01.T01:CDS | 55.0% | |
| ATGCGCTCGCCCAGCGAGAA+AGG | + | contig169end:13658-13677 | None:intergenic | 65.0% | |
| AGGGTCGGGGCCTTTCTCGC+TGG | - | contig169end:13645-13664 | MsG0080048009.01.T01:CDS | 70.0% | |
| GGGTCGGGGCCTTTCTCGCT+GGG | - | contig169end:13646-13665 | MsG0080048009.01.T01:CDS | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| contig169end | gene | 13286 | 13720 | 13286 | ID=MsG0080048009.01;Name=MsG0080048009.01 |
| contig169end | mRNA | 13286 | 13720 | 13286 | ID=MsG0080048009.01.T01;Parent=MsG0080048009.01;Name=MsG0080048009.01.T01;_AED=0.48;_eAED=0.56;_QI=0|0|0|1|1|1|2|0|113 |
| contig169end | exon | 13453 | 13720 | 13453 | ID=MsG0080048009.01.T01:exon:1041;Parent=MsG0080048009.01.T01 |
| contig169end | exon | 13286 | 13359 | 13286 | ID=MsG0080048009.01.T01:exon:1042;Parent=MsG0080048009.01.T01 |
| contig169end | CDS | 13453 | 13720 | 13453 | ID=MsG0080048009.01.T01:cds;Parent=MsG0080048009.01.T01 |
| contig169end | CDS | 13286 | 13359 | 13286 | ID=MsG0080048009.01.T01:cds;Parent=MsG0080048009.01.T01 |
| Gene Sequence |
| Protein sequence |
