Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048008.01.T01 | YP_009237628.1 | 100 | 195 | 0 | 0 | 1 | 195 | 1 | 195 | 1.01E-137 | 392 |
| MsG0080048008.01.T02 | AGC78858.1 | 100 | 188 | 0 | 0 | 1 | 188 | 8 | 195 | 1.57E-132 | 379 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048008.01.T01 | P09003 | 89.231 | 195 | 21 | 0 | 1 | 195 | 1 | 195 | 2.11E-123 | 349 |
| MsG0080048008.01.T02 | P09003 | 88.83 | 188 | 21 | 0 | 1 | 188 | 8 | 195 | 3.59E-118 | 335 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048008.01.T01 | A0A126TGU9 | 100.000 | 195 | 0 | 0 | 1 | 195 | 1 | 195 | 4.81e-138 | 392 |
| MsG0080048008.01.T02 | R4IV24 | 100.000 | 188 | 0 | 0 | 1 | 188 | 8 | 195 | 7.52e-133 | 379 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048008.01 | MsG0080048009.01 | 0.840368 | 9.070175e-58 | 1.289214e-54 |
| MsG0080048008.01 | MsG0080048011.01 | 0.853546 | 2.225319e-61 | 4.825716e-58 |
| MsG0080048008.01 | MsG0080048567.01 | 0.843581 | 1.282824e-58 | 2.016326e-55 |
| MsG0080048008.01 | MsG0080049160.01 | 0.847608 | 1.038354e-59 | 1.855624e-56 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048008.01.T01 | ATMG00640 | 89.474 | 190 | 20 | 0 | 1 | 190 | 1 | 190 | 3.89e-121 | 341 |
| MsG0080048008.01.T02 | ATMG00640 | 89.071 | 183 | 20 | 0 | 1 | 183 | 8 | 190 | 7.63e-116 | 327 |
Find 46 sgRNAs with CRISPR-Local
Find 48 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCCCGTCGCATCCGTCTTC+AGG | 0.290398 | contig169end:-12815 | MsG0080048008.01.T01:CDS |
| CAAAGTTTCACTTATTAGTT+AGG | 0.300631 | contig169end:-12780 | MsG0080048008.01.T01:CDS |
| CTTAAGAATGGTTCGGGTGG+GGG | 0.309576 | contig169end:-12689 | MsG0080048008.01.T01:CDS |
| GTGTTGCTGACTTAACATTT+AGG | 0.316096 | contig169end:+12854 | None:intergenic |
| CGGGAGAATCCAGGCTATTC+AGG | 0.327183 | contig169end:-13049 | MsG0080048008.01.T01:CDS |
| CTGACTTAACATTTAGGTTT+CGG | 0.330980 | contig169end:+12860 | None:intergenic |
| ATATTCAGTCGGAAGAGTTT+AGG | 0.344405 | contig169end:-13096 | MsG0080048008.01.T01:CDS |
| GAACTTAATGCACAAATAGA+TGG | 0.361910 | contig169end:+13180 | None:intergenic |
| CGTTGTTGTTCATTAGATTC+CGG | 0.380525 | contig169end:+12985 | None:intergenic |
| GGAACTACTTCGTTAGGATT+GGG | 0.382567 | contig169end:+13009 | None:intergenic |
| TCTTAAGAATGGTTCGGGTG+GGG | 0.387129 | contig169end:-12690 | MsG0080048008.01.T01:CDS |
| TTAAGAATGGTTCGGGTGGG+GGG | 0.390162 | contig169end:-12688 | MsG0080048008.01.T01:CDS |
| TGGTGGTCTTAAGAATGGTT+CGG | 0.393767 | contig169end:-12696 | MsG0080048008.01.T01:CDS |
| CGACGGGAAGAAGTGGCATT+TGG | 0.400338 | contig169end:+12829 | None:intergenic |
| GGTGGTCTTAAGAATGGTTC+GGG | 0.403561 | contig169end:-12695 | MsG0080048008.01.T01:CDS |
| CTTTCTAGCTTGCATATTCG+TGG | 0.405255 | contig169end:+13221 | None:intergenic |
| AGGAACTACTTCGTTAGGAT+TGG | 0.427197 | contig169end:+13008 | None:intergenic |
| GTCTTAAGAATGGTTCGGGT+GGG | 0.431263 | contig169end:-12691 | MsG0080048008.01.T01:CDS |
| AGGATCAGTTTGCGAATTTG+TGG | 0.443491 | contig169end:-12955 | MsG0080048008.01.T01:CDS |
| TCCGGAGGAACTACTTCGTT+AGG | 0.464415 | contig169end:+13003 | None:intergenic |
| GCGATTCTTCCTGAATAGCC+TGG | 0.465368 | contig169end:+13040 | None:intergenic |
| TTTCTGCTTTCGAGATACAC+TGG | 0.466843 | contig169end:+12743 | None:intergenic |
| TTAGGCGCACAGCGTGCCAT+TGG | 0.478556 | contig169end:+12913 | None:intergenic |
| CACTCTCGACGGGAGAATCC+AGG | 0.485324 | contig169end:-13058 | MsG0080048008.01.T01:CDS |
| GGTCTTAAGAATGGTTCGGG+TGG | 0.486499 | contig169end:-12692 | MsG0080048008.01.T01:CDS |
| TTCTGCTTTCGAGATACACT+GGG | 0.491411 | contig169end:+12744 | None:intergenic |
| GAGCTTGGTGGTCTTAAGAA+TGG | 0.493363 | contig169end:-12701 | MsG0080048008.01.T01:CDS |
| TCCTAACGAAGTAGTTCCTC+CGG | 0.503653 | contig169end:-13004 | MsG0080048008.01.T01:CDS |
| ATCCGTCTTCAGGACGATCT+AGG | 0.506528 | contig169end:-12805 | MsG0080048008.01.T01:CDS |
| AGAACTCATTCGAGAGAGCT+TGG | 0.513456 | contig169end:-12716 | MsG0080048008.01.T01:CDS |
| ATGAACAACAACGATTACTT+AGG | 0.515235 | contig169end:-12975 | MsG0080048008.01.T01:CDS |
| AGTTTCACTTATTAGTTAGG+AGG | 0.540802 | contig169end:-12777 | MsG0080048008.01.T01:CDS |
| GAACTACTTCGTTAGGATTG+GGG | 0.553593 | contig169end:+13010 | None:intergenic |
| CGTCCTGAAGACGGATGCGA+CGG | 0.557091 | contig169end:+12812 | None:intergenic |
| ACGGATGCGACGGGAAGAAG+TGG | 0.567388 | contig169end:+12822 | None:intergenic |
| CTTTCAAAGTCACTCTCGAC+GGG | 0.569214 | contig169end:-13068 | MsG0080048008.01.T01:CDS |
| TGCCTAGATCGTCCTGAAGA+CGG | 0.584599 | contig169end:+12803 | None:intergenic |
| TAGGCTTCATCATATTCAGT+CGG | 0.597735 | contig169end:-13107 | MsG0080048008.01.T01:CDS |
| CATTGGTAATGATTCTACTA+CGG | 0.601302 | contig169end:+12930 | None:intergenic |
| ACTTTCAAAGTCACTCTCGA+CGG | 0.612782 | contig169end:-13069 | MsG0080048008.01.T01:CDS |
| ACTCATTCGAGAGAGCTTGG+TGG | 0.620569 | contig169end:-12713 | MsG0080048008.01.T01:CDS |
| GTCCTGAAGACGGATGCGAC+GGG | 0.631883 | contig169end:+12813 | None:intergenic |
| CGTAGTAGAATCATTACCAA+TGG | 0.631929 | contig169end:-12929 | MsG0080048008.01.T01:CDS |
| TGTTGTTCATTAGATTCCGG+AGG | 0.656384 | contig169end:+12988 | None:intergenic |
| CTGCTTTCGAGATACACTGG+GGG | 0.667482 | contig169end:+12746 | None:intergenic |
| TCTGCTTTCGAGATACACTG+GGG | 0.707221 | contig169end:+12745 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | CAAAGTTTCACTTATTAGTT+AGG | - | contig169end:13123-13142 | MsG0080048008.01.T01:CDS | 25.0% |
| AGTTTCACTTATTAGTTAGG+AGG | - | contig169end:13126-13145 | MsG0080048008.01.T01:CDS | 30.0% | |
| ATGAACAACAACGATTACTT+AGG | - | contig169end:12928-12947 | MsG0080048008.01.T01:CDS | 30.0% | |
| GAACTTAATGCACAAATAGA+TGG | + | contig169end:12726-12745 | None:intergenic | 30.0% | |
| ! | CTGACTTAACATTTAGGTTT+CGG | + | contig169end:13046-13065 | None:intergenic | 30.0% |
| !! | ATAGTAGCTCGTTGTTTTAT+AGG | - | contig169end:12777-12796 | MsG0080048008.01.T01:CDS | 30.0% |
| !! | CATTGGTAATGATTCTACTA+CGG | + | contig169end:12976-12995 | None:intergenic | 30.0% |
| ATATTCAGTCGGAAGAGTTT+AGG | - | contig169end:12807-12826 | MsG0080048008.01.T01:CDS | 35.0% | |
| CGTAGTAGAATCATTACCAA+TGG | - | contig169end:12974-12993 | MsG0080048008.01.T01:CDS | 35.0% | |
| TAGGCTTCATCATATTCAGT+CGG | - | contig169end:12796-12815 | MsG0080048008.01.T01:CDS | 35.0% | |
| ! | CGTTGTTGTTCATTAGATTC+CGG | + | contig169end:12921-12940 | None:intergenic | 35.0% |
| !! | GTGTTGCTGACTTAACATTT+AGG | + | contig169end:13052-13071 | None:intergenic | 35.0% |
| ACTTTCAAAGTCACTCTCGA+CGG | - | contig169end:12834-12853 | MsG0080048008.01.T01:CDS | 40.0% | |
| AGGAACTACTTCGTTAGGAT+TGG | + | contig169end:12898-12917 | None:intergenic | 40.0% | |
| AGGATCAGTTTGCGAATTTG+TGG | - | contig169end:12948-12967 | MsG0080048008.01.T01:CDS | 40.0% | |
| GAACTACTTCGTTAGGATTG+GGG | + | contig169end:12896-12915 | None:intergenic | 40.0% | |
| GGAACTACTTCGTTAGGATT+GGG | + | contig169end:12897-12916 | None:intergenic | 40.0% | |
| TGTTGTTCATTAGATTCCGG+AGG | + | contig169end:12918-12937 | None:intergenic | 40.0% | |
| TTCTGCTTTCGAGATACACT+GGG | + | contig169end:13162-13181 | None:intergenic | 40.0% | |
| TTTCTGCTTTCGAGATACAC+TGG | + | contig169end:13163-13182 | None:intergenic | 40.0% | |
| ! | TGGTGGTCTTAAGAATGGTT+CGG | - | contig169end:13207-13226 | MsG0080048008.01.T01:CDS | 40.0% |
| !! | CTTTCTAGCTTGCATATTCG+TGG | + | contig169end:12685-12704 | None:intergenic | 40.0% |
| AGAACTCATTCGAGAGAGCT+TGG | - | contig169end:13187-13206 | MsG0080048008.01.T01:CDS | 45.0% | |
| CTTTCAAAGTCACTCTCGAC+GGG | - | contig169end:12835-12854 | MsG0080048008.01.T01:CDS | 45.0% | |
| TCCTAACGAAGTAGTTCCTC+CGG | - | contig169end:12899-12918 | MsG0080048008.01.T01:CDS | 45.0% | |
| TCTGCTTTCGAGATACACTG+GGG | + | contig169end:13161-13180 | None:intergenic | 45.0% | |
| ! | GAGCTTGGTGGTCTTAAGAA+TGG | - | contig169end:13202-13221 | MsG0080048008.01.T01:CDS | 45.0% |
| ! | GGTGGTCTTAAGAATGGTTC+GGG | - | contig169end:13208-13227 | MsG0080048008.01.T01:CDS | 45.0% |
| ! | GTCTTAAGAATGGTTCGGGT+GGG | - | contig169end:13212-13231 | MsG0080048008.01.T01:CDS | 45.0% |
| ! | TCTTAAGAATGGTTCGGGTG+GGG | - | contig169end:13213-13232 | MsG0080048008.01.T01:CDS | 45.0% |
| ! | TGCACTGTCTTTTCGCACTT+AGG | + | contig169end:13011-13030 | None:intergenic | 45.0% |
| ACTCATTCGAGAGAGCTTGG+TGG | - | contig169end:13190-13209 | MsG0080048008.01.T01:CDS | 50.0% | |
| ATCCGTCTTCAGGACGATCT+AGG | - | contig169end:13098-13117 | MsG0080048008.01.T01:CDS | 50.0% | |
| CTGCTTTCGAGATACACTGG+GGG | + | contig169end:13160-13179 | None:intergenic | 50.0% | |
| GCGATTCTTCCTGAATAGCC+TGG | + | contig169end:12866-12885 | None:intergenic | 50.0% | |
| TCCGGAGGAACTACTTCGTT+AGG | + | contig169end:12903-12922 | None:intergenic | 50.0% | |
| TGCCTAGATCGTCCTGAAGA+CGG | + | contig169end:13103-13122 | None:intergenic | 50.0% | |
| ! | CTTAAGAATGGTTCGGGTGG+GGG | - | contig169end:13214-13233 | MsG0080048008.01.T01:CDS | 50.0% |
| ! | GGTCTTAAGAATGGTTCGGG+TGG | - | contig169end:13211-13230 | MsG0080048008.01.T01:CDS | 50.0% |
| ! | TTAAGAATGGTTCGGGTGGG+GGG | - | contig169end:13215-13234 | MsG0080048008.01.T01:CDS | 50.0% |
| CGACGGGAAGAAGTGGCATT+TGG | + | contig169end:13077-13096 | None:intergenic | 55.0% | |
| CGGGAGAATCCAGGCTATTC+AGG | - | contig169end:12854-12873 | MsG0080048008.01.T01:CDS | 55.0% | |
| ACGGATGCGACGGGAAGAAG+TGG | + | contig169end:13084-13103 | None:intergenic | 60.0% | |
| CACTCTCGACGGGAGAATCC+AGG | - | contig169end:12845-12864 | MsG0080048008.01.T01:CDS | 60.0% | |
| CGTCCTGAAGACGGATGCGA+CGG | + | contig169end:13094-13113 | None:intergenic | 60.0% | |
| GTCCTGAAGACGGATGCGAC+GGG | + | contig169end:13093-13112 | None:intergenic | 60.0% | |
| TTAGGCGCACAGCGTGCCAT+TGG | + | contig169end:12993-13012 | None:intergenic | 60.0% | |
| TTCCCGTCGCATCCGTCTTC+AGG | - | contig169end:13088-13107 | MsG0080048008.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| contig169end | gene | 12669 | 13256 | 12669 | ID=MsG0080048008.01;Name=MsG0080048008.01 |
| contig169end | mRNA | 12669 | 13256 | 12669 | ID=MsG0080048008.01.T01;Parent=MsG0080048008.01;Name=MsG0080048008.01.T01;_AED=0.39;_eAED=0.39;_QI=0|-1|0|1|-1|0|1|0|195 |
| contig169end | exon | 12669 | 13256 | 12669 | ID=MsG0080048008.01.T01:exon:17806;Parent=MsG0080048008.01.T01 |
| contig169end | CDS | 12669 | 13256 | 12669 | ID=MsG0080048008.01.T01:cds;Parent=MsG0080048008.01.T01 |
| contig169end | mRNA | 12669 | 13235 | 12669 | ID=MsG0080048008.01.T02;Parent=MsG0080048008.01;Name=MsG0080048008.01.T02;_AED=0.40;_eAED=0.40;_QI=0|-1|0|1|-1|0|1|0|188 |
| contig169end | exon | 12669 | 13235 | 12669 | ID=MsG0080048008.01.T02:exon:17807;Parent=MsG0080048008.01.T02 |
| contig169end | CDS | 12669 | 13235 | 12669 | ID=MsG0080048008.01.T02:cds;Parent=MsG0080048008.01.T02 |
| contig169end | mRNA | 12806 | 12871 | 12806 | ID=MsG0080048008.01.T03;Parent=MsG0080048008.01;Name=MsG0080048008.01.T03;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|0|1|0|21 |
| contig169end | exon | 12806 | 12871 | 12806 | ID=MsG0080048008.01.T03:exon:17808;Parent=MsG0080048008.01.T03 |
| contig169end | CDS | 12806 | 12871 | 12806 | ID=MsG0080048008.01.T03:cds;Parent=MsG0080048008.01.T03 |
| Gene Sequence |
| Protein sequence |
