Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048027.01.T01 | XP_013452513.1 | 75.882 | 170 | 6 | 1 | 1 | 135 | 100 | 269 | 5.90E-82 | 254 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048027.01.T01 | Q9LVB8 | 47.024 | 168 | 53 | 2 | 1 | 133 | 100 | 266 | 3.61E-44 | 150 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048027.01.T01 | A0A072UAU3 | 75.882 | 170 | 6 | 1 | 1 | 135 | 100 | 269 | 2.82e-82 | 254 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048027.01 | MsG0480022736.01 | 0.825414 | 4.749761e-54 | 4.326175e-51 |
| MsG0080048027.01 | MsG0680033953.01 | 0.806298 | 8.805640e-50 | 4.762365e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048027.01.T01 | MTR_6g464280 | 75.882 | 170 | 6 | 1 | 1 | 135 | 100 | 269 | 7.14e-86 | 254 |
| MsG0080048027.01.T01 | MTR_6g464320 | 65.089 | 169 | 24 | 1 | 2 | 135 | 102 | 270 | 5.75e-70 | 214 |
| MsG0080048027.01.T01 | MTR_6g464510 | 57.988 | 169 | 35 | 2 | 1 | 134 | 103 | 270 | 1.08e-59 | 187 |
| MsG0080048027.01.T01 | MTR_1g102160 | 48.000 | 175 | 47 | 4 | 1 | 134 | 98 | 269 | 4.52e-44 | 147 |
| MsG0080048027.01.T01 | MTR_1g102170 | 43.713 | 167 | 58 | 2 | 2 | 133 | 116 | 281 | 1.13e-39 | 136 |
| MsG0080048027.01.T01 | MTR_0007s0520 | 44.118 | 170 | 57 | 3 | 3 | 134 | 100 | 269 | 9.88e-39 | 134 |
| MsG0080048027.01.T01 | MTR_6g464120 | 44.706 | 170 | 56 | 3 | 1 | 134 | 102 | 269 | 8.53e-37 | 132 |
| MsG0080048027.01.T01 | MTR_8g035520 | 41.718 | 163 | 55 | 2 | 2 | 124 | 100 | 262 | 2.29e-33 | 120 |
| MsG0080048027.01.T01 | MTR_8g035540 | 42.953 | 149 | 46 | 1 | 1 | 110 | 91 | 239 | 3.26e-32 | 117 |
| MsG0080048027.01.T01 | MTR_0007s0480 | 50.000 | 96 | 47 | 1 | 40 | 134 | 20 | 115 | 1.74e-25 | 95.9 |
| MsG0080048027.01.T01 | MTR_8g035500 | 38.095 | 147 | 51 | 2 | 3 | 110 | 96 | 241 | 2.92e-25 | 98.6 |
| MsG0080048027.01.T01 | MTR_7g406880 | 34.857 | 175 | 59 | 6 | 2 | 126 | 110 | 279 | 5.30e-22 | 90.1 |
| MsG0080048027.01.T01 | MTR_6g065430 | 33.775 | 151 | 64 | 2 | 2 | 118 | 110 | 258 | 1.03e-21 | 89.4 |
| MsG0080048027.01.T01 | MTR_6g065270 | 33.113 | 151 | 68 | 2 | 1 | 118 | 116 | 266 | 6.34e-19 | 81.6 |
| MsG0080048027.01.T01 | MTR_6g065110 | 31.544 | 149 | 70 | 2 | 2 | 118 | 117 | 265 | 1.59e-18 | 80.5 |
| MsG0080048027.01.T01 | MTR_6g065120 | 33.103 | 145 | 65 | 3 | 6 | 118 | 122 | 266 | 2.12e-17 | 77.4 |
| MsG0080048027.01.T01 | MTR_1g111280 | 32.903 | 155 | 58 | 3 | 2 | 110 | 109 | 263 | 4.98e-17 | 76.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048027.01.T01 | AT5G62180 | 47.024 | 168 | 53 | 2 | 1 | 133 | 100 | 266 | 3.68e-45 | 150 |
| MsG0080048027.01.T01 | AT2G45600 | 40.491 | 163 | 51 | 2 | 2 | 120 | 87 | 247 | 3.52e-32 | 117 |
| MsG0080048027.01.T01 | AT2G45610 | 41.667 | 144 | 45 | 2 | 6 | 110 | 104 | 247 | 1.34e-29 | 110 |
| MsG0080048027.01.T01 | AT5G06570 | 34.416 | 154 | 52 | 3 | 2 | 110 | 112 | 261 | 8.55e-20 | 84.0 |
| MsG0080048027.01.T01 | AT5G06570 | 34.416 | 154 | 52 | 3 | 2 | 110 | 112 | 261 | 8.55e-20 | 84.0 |
| MsG0080048027.01.T01 | AT5G06570 | 34.416 | 154 | 52 | 3 | 2 | 110 | 97 | 246 | 8.57e-20 | 84.0 |
| MsG0080048027.01.T01 | AT5G06570 | 34.416 | 154 | 52 | 3 | 2 | 110 | 97 | 246 | 8.57e-20 | 84.0 |
| MsG0080048027.01.T01 | AT1G68620 | 37.500 | 144 | 52 | 4 | 2 | 108 | 110 | 252 | 5.98e-18 | 79.0 |
Find 42 sgRNAs with CRISPR-Local
Find 54 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATCATGAGTATTCCAATCTA+AGG | 0.274329 | contig175end:-9675 | MsG0080048027.01.T01:CDS |
| CATTCTTTGGTGGGACCAAT+AGG | 0.344213 | contig175end:-9795 | MsG0080048027.01.T01:CDS |
| ATATTGCGTCAACCATTCTT+TGG | 0.351388 | contig175end:-9808 | MsG0080048027.01.T01:CDS |
| TCCATTGGTTGATCGTAATA+AGG | 0.354913 | contig175end:-9573 | MsG0080048027.01.T01:CDS |
| ACCATTCCCAACCCTTAGAT+TGG | 0.372685 | contig175end:+9663 | MsG0080048027.01.T01:intergenic |
| ATCAAATCACTAACACATAA+AGG | 0.393922 | contig175end:+9733 | MsG0080048027.01.T01:intergenic |
| CTTGAGCCGTTGAAAATTCA+AGG | 0.398268 | contig175end:-9835 | MsG0080048027.01.T01:CDS |
| GCCGCCGGTAGCCGGTGCTC+CGG | 0.443546 | contig175end:+10017 | MsG0080048027.01.T01:intergenic |
| CAACTCTGACTCAGTCCTAT+TGG | 0.445819 | contig175end:+9780 | MsG0080048027.01.T01:intergenic |
| CATCATATGCCGCCGGTAGC+CGG | 0.451594 | contig175end:+10009 | MsG0080048027.01.T01:intergenic |
| TGATCACGGTTAACACCAAT+TGG | 0.457086 | contig175end:+9694 | MsG0080048027.01.T01:intergenic |
| AGTATTCCAATCTAAGGGTT+GGG | 0.474229 | contig175end:-9669 | MsG0080048027.01.T01:CDS |
| CGCACCGGAGCACCGGCTAC+CGG | 0.478223 | contig175end:-10021 | MsG0080048027.01.T01:CDS |
| ATCGCCTCGCACCGGAGCAC+CGG | 0.497130 | contig175end:-10028 | MsG0080048027.01.T01:CDS |
| TGGGAGTTAGCGTTACCAAT+TGG | 0.507682 | contig175end:-9709 | MsG0080048027.01.T01:CDS |
| CGTCGAAGCAGTTGTCGCCT+CGG | 0.508826 | contig175end:-10057 | MsG0080048027.01.T01:CDS |
| TGCGTCAACCATTCTTTGGT+GGG | 0.516166 | contig175end:-9804 | MsG0080048027.01.T01:CDS |
| ATGTGTTAGTGATTTGATGT+GGG | 0.517094 | contig175end:-9728 | MsG0080048027.01.T01:CDS |
| CTAAGATGAAGGATCATGAA+TGG | 0.519206 | contig175end:-9624 | MsG0080048027.01.T01:CDS |
| GAGGGTGTTGGTGAGTATGA+TGG | 0.520421 | contig175end:-9602 | MsG0080048027.01.T01:CDS |
| CGAGGCGACAACTGCTTCGA+CGG | 0.523045 | contig175end:+10058 | MsG0080048027.01.T01:intergenic |
| TATGTGTTAGTGATTTGATG+TGG | 0.530358 | contig175end:-9729 | MsG0080048027.01.T01:CDS |
| TTGCGTCAACCATTCTTTGG+TGG | 0.533414 | contig175end:-9805 | MsG0080048027.01.T01:CDS |
| GGTTGATCGTAATAAGGAGT+TGG | 0.536543 | contig175end:-9567 | MsG0080048027.01.T01:CDS |
| GAGTATTCCAATCTAAGGGT+TGG | 0.553194 | contig175end:-9670 | MsG0080048027.01.T01:CDS |
| CTATTGGTCCCACCAAAGAA+TGG | 0.558746 | contig175end:+9796 | MsG0080048027.01.T01:intergenic |
| GGTGTTGGTGAGTATGATGG+TGG | 0.561719 | contig175end:-9599 | MsG0080048027.01.T01:CDS |
| GGTCGATTATCGCCTCGCAC+CGG | 0.575927 | contig175end:-10036 | MsG0080048027.01.T01:CDS |
| GTAGCCGGTGCTCCGGTGCG+AGG | 0.576122 | contig175end:+10024 | MsG0080048027.01.T01:intergenic |
| TTGAGCCGTTGAAAATTCAA+GGG | 0.578065 | contig175end:-9834 | MsG0080048027.01.T01:CDS |
| GGCGGCATATGATGATGCTA+TGG | 0.579015 | contig175end:-10000 | MsG0080048027.01.T01:CDS |
| TCCAATCTAAGGGTTGGGAA+TGG | 0.580920 | contig175end:-9664 | MsG0080048027.01.T01:CDS |
| ACCGGAGCACCGGCTACCGG+CGG | 0.597839 | contig175end:-10018 | MsG0080048027.01.T01:CDS |
| GTATGATGGTGGAGATCCAT+TGG | 0.600241 | contig175end:-9588 | MsG0080048027.01.T01:CDS |
| GGATCATGAATGGAGGGTGT+TGG | 0.601146 | contig175end:-9614 | MsG0080048027.01.T01:CDS |
| GATTGGAATACTCATGATCA+CGG | 0.602165 | contig175end:+9680 | MsG0080048027.01.T01:intergenic |
| GATGAAGGATCATGAATGGA+GGG | 0.604665 | contig175end:-9620 | MsG0080048027.01.T01:CDS |
| TCATGAGTATTCCAATCTAA+GGG | 0.620597 | contig175end:-9674 | MsG0080048027.01.T01:CDS |
| TCCTTATTACGATCAACCAA+TGG | 0.629948 | contig175end:+9572 | MsG0080048027.01.T01:intergenic |
| AGATGAAGGATCATGAATGG+AGG | 0.637933 | contig175end:-9621 | MsG0080048027.01.T01:CDS |
| ATAGCATCATCATATGCCGC+CGG | 0.642105 | contig175end:+10002 | None:intergenic |
| TGCGAGGCGATAATCGACCG+AGG | 0.723884 | contig175end:+10040 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ATCAAATCACTAACACATAA+AGG | + | contig175end:9926-9945 | MsG0080048027.01.T01:intergenic | 25.0% |
| !! | ACTTTTCAAATGTCTTCTAA+TGG | - | contig175end:9733-9752 | MsG0080048027.01.T01:CDS | 25.0% |
| !! | ATTAACCCTTGAATTTTCAA+CGG | + | contig175end:9830-9849 | MsG0080048027.01.T01:intergenic | 25.0% |
| !! | CTTTTCAAATGTCTTCTAAT+GGG | - | contig175end:9734-9753 | MsG0080048027.01.T01:CDS | 25.0% |
| !!! | TGAAAAATTTGCTAAGATGA+AGG | - | contig175end:10021-10040 | MsG0080048027.01.T01:CDS | 25.0% |
| ATCATGAGTATTCCAATCTA+AGG | - | contig175end:9981-10000 | MsG0080048027.01.T01:intron | 30.0% | |
| ATGTGTTAGTGATTTGATGT+GGG | - | contig175end:9928-9947 | MsG0080048027.01.T01:intron | 30.0% | |
| CCATTTCAGAACAAAAATTG+TGG | + | contig175end:9572-9591 | MsG0080048027.01.T01:intergenic | 30.0% | |
| CTAACACATAAAGGAAAAAC+AGG | + | contig175end:9917-9936 | MsG0080048027.01.T01:intergenic | 30.0% | |
| TATGTGTTAGTGATTTGATG+TGG | - | contig175end:9927-9946 | MsG0080048027.01.T01:intron | 30.0% | |
| TCATGAGTATTCCAATCTAA+GGG | - | contig175end:9982-10001 | MsG0080048027.01.T01:intron | 30.0% | |
| !!! | CAATTTTTGTTCTGAAATGG+CGG | - | contig175end:9572-9591 | MsG0080048027.01.T01:CDS | 30.0% |
| !!! | CCACAATTTTTGTTCTGAAA+TGG | - | contig175end:9569-9588 | MsG0080048027.01.T01:CDS | 30.0% |
| AGTATTCCAATCTAAGGGTT+GGG | - | contig175end:9987-10006 | MsG0080048027.01.T01:intron | 35.0% | |
| ATATTGCGTCAACCATTCTT+TGG | - | contig175end:9848-9867 | MsG0080048027.01.T01:CDS | 35.0% | |
| CTAAGATGAAGGATCATGAA+TGG | - | contig175end:10032-10051 | MsG0080048027.01.T01:CDS | 35.0% | |
| GATTGGAATACTCATGATCA+CGG | + | contig175end:9979-9998 | MsG0080048027.01.T01:intergenic | 35.0% | |
| TCCTTATTACGATCAACCAA+TGG | + | contig175end:10087-10106 | MsG0080048027.01.T01:intergenic | 35.0% | |
| TTGAGCCGTTGAAAATTCAA+GGG | - | contig175end:9822-9841 | MsG0080048027.01.T01:CDS | 35.0% | |
| ! | TCCATTGGTTGATCGTAATA+AGG | - | contig175end:10083-10102 | MsG0080048027.01.T01:CDS | 35.0% |
| ! | TTAGGTCAAGTGATGATGAA+TGG | - | contig175end:9694-9713 | MsG0080048027.01.T01:CDS | 35.0% |
| !! | TGGAAGCATTGAGTTTGATT+AGG | - | contig175end:9676-9695 | MsG0080048027.01.T01:CDS | 35.0% |
| AGATGAAGGATCATGAATGG+AGG | - | contig175end:10035-10054 | MsG0080048027.01.T01:CDS | 40.0% | |
| CTTCTAATGGGAAATAGTGC+TGG | - | contig175end:9746-9765 | MsG0080048027.01.T01:CDS | 40.0% | |
| CTTGAGCCGTTGAAAATTCA+AGG | - | contig175end:9821-9840 | MsG0080048027.01.T01:CDS | 40.0% | |
| GAGTATTCCAATCTAAGGGT+TGG | - | contig175end:9986-10005 | MsG0080048027.01.T01:intron | 40.0% | |
| GATGAAGGATCATGAATGGA+GGG | - | contig175end:10036-10055 | MsG0080048027.01.T01:CDS | 40.0% | |
| GCTACAATTGCATATCATGC+AGG | - | contig175end:9770-9789 | MsG0080048027.01.T01:CDS | 40.0% | |
| GGTTGATCGTAATAAGGAGT+TGG | - | contig175end:10089-10108 | MsG0080048027.01.T01:CDS | 40.0% | |
| TGATCACGGTTAACACCAAT+TGG | + | contig175end:9965-9984 | MsG0080048027.01.T01:intergenic | 40.0% | |
| ACCATTCCCAACCCTTAGAT+TGG | + | contig175end:9996-10015 | MsG0080048026.01.T01:intergenic | 45.0% | |
| ATAGCATCATCATATGCCGC+CGG | + | contig175end:9657-9676 | MsG0080048027.01.T01:intergenic | 45.0% | |
| CAACTCTGACTCAGTCCTAT+TGG | + | contig175end:9879-9898 | MsG0080048027.01.T01:intergenic | 45.0% | |
| CATTCTTTGGTGGGACCAAT+AGG | - | contig175end:9861-9880 | MsG0080048027.01.T01:CDS | 45.0% | |
| CTATTGGTCCCACCAAAGAA+TGG | + | contig175end:9863-9882 | MsG0080048027.01.T01:intergenic | 45.0% | |
| TCCAATCTAAGGGTTGGGAA+TGG | - | contig175end:9992-10011 | MsG0080048027.01.T01:CDS | 45.0% | |
| TGCGTCAACCATTCTTTGGT+GGG | - | contig175end:9852-9871 | MsG0080048027.01.T01:CDS | 45.0% | |
| TGGGAGTTAGCGTTACCAAT+TGG | - | contig175end:9947-9966 | MsG0080048027.01.T01:intron | 45.0% | |
| TTGCGTCAACCATTCTTTGG+TGG | - | contig175end:9851-9870 | MsG0080048027.01.T01:CDS | 45.0% | |
| !! | GTATGATGGTGGAGATCCAT+TGG | - | contig175end:10068-10087 | MsG0080048027.01.T01:CDS | 45.0% |
| ! | GGATCATGAATGGAGGGTGT+TGG | - | contig175end:10042-10061 | MsG0080048027.01.T01:CDS | 50.0% |
| ! | GGCGGCATATGATGATGCTA+TGG | - | contig175end:9656-9675 | MsG0080048027.01.T01:CDS | 50.0% |
| !! | GAGGGTGTTGGTGAGTATGA+TGG | - | contig175end:10054-10073 | MsG0080048027.01.T01:CDS | 50.0% |
| !! | GGTGTTGGTGAGTATGATGG+TGG | - | contig175end:10057-10076 | MsG0080048027.01.T01:CDS | 50.0% |
| CATCATATGCCGCCGGTAGC+CGG | + | contig175end:9650-9669 | MsG0080048027.01.T01:intergenic | 60.0% | |
| CGAGGCGACAACTGCTTCGA+CGG | + | contig175end:9601-9620 | MsG0080048027.01.T01:intergenic | 60.0% | |
| CGTCGAAGCAGTTGTCGCCT+CGG | - | contig175end:9599-9618 | MsG0080048027.01.T01:CDS | 60.0% | |
| GGTCGATTATCGCCTCGCAC+CGG | - | contig175end:9620-9639 | MsG0080048027.01.T01:CDS | 60.0% | |
| TGCGAGGCGATAATCGACCG+AGG | + | contig175end:9619-9638 | MsG0080048027.01.T01:intergenic | 60.0% | |
| ATCGCCTCGCACCGGAGCAC+CGG | - | contig175end:9628-9647 | MsG0080048027.01.T01:CDS | 70.0% | |
| GTAGCCGGTGCTCCGGTGCG+AGG | + | contig175end:9635-9654 | MsG0080048027.01.T01:intergenic | 75.0% | |
| !! | ACCGGAGCACCGGCTACCGG+CGG | - | contig175end:9638-9657 | MsG0080048027.01.T01:CDS | 75.0% |
| !! | CGCACCGGAGCACCGGCTAC+CGG | - | contig175end:9635-9654 | MsG0080048027.01.T01:CDS | 75.0% |
| ! | GCCGCCGGTAGCCGGTGCTC+CGG | + | contig175end:9642-9661 | MsG0080048027.01.T01:intergenic | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| contig175end | gene | 9564 | 10114 | 9564 | ID=MsG0080048027.01;Name=MsG0080048027.01 |
| contig175end | mRNA | 9564 | 10114 | 9564 | ID=MsG0080048027.01.T01;Parent=MsG0080048027.01;Name=MsG0080048027.01.T01;_AED=0.35;_eAED=0.35;_QI=0|0|0|1|1|1|2|0|148 |
| contig175end | exon | 9991 | 10114 | 9991 | ID=MsG0080048027.01.T01:exon:2045;Parent=MsG0080048027.01.T01 |
| contig175end | exon | 9564 | 9886 | 9564 | ID=MsG0080048027.01.T01:exon:2046;Parent=MsG0080048027.01.T01 |
| contig175end | CDS | 9991 | 10114 | 9991 | ID=MsG0080048027.01.T01:cds;Parent=MsG0080048027.01.T01 |
| contig175end | CDS | 9564 | 9886 | 9564 | ID=MsG0080048027.01.T01:cds;Parent=MsG0080048027.01.T01 |
| Gene Sequence |
| Protein sequence |
