Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048100.01.T05 | XP_024632136.1 | 89.61 | 77 | 8 | 0 | 1 | 77 | 133 | 209 | 4.54E-42 | 148 |
| MsG0080048100.01.T04 | XP_024632136.1 | 90.323 | 93 | 9 | 0 | 1 | 93 | 117 | 209 | 6.51E-54 | 179 |
| MsG0080048100.01.T03 | XP_024632136.1 | 90.566 | 106 | 10 | 0 | 1 | 106 | 104 | 209 | 2.06E-62 | 201 |
| MsG0080048100.01.T02 | XP_024632136.1 | 86.62 | 142 | 19 | 0 | 1 | 142 | 68 | 209 | 4.69E-81 | 250 |
| MsG0080048100.01.T01 | KEH41170.1 | 54.198 | 131 | 30 | 1 | 1 | 101 | 1 | 131 | 1.26E-39 | 145 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048100.01.T02 | P0C2F6 | 30.37 | 135 | 94 | 0 | 1 | 135 | 4 | 138 | 8.93E-18 | 83.2 |
| MsG0080048100.01.T03 | P93295 | 37.113 | 97 | 60 | 1 | 8 | 103 | 2 | 98 | 1.75E-19 | 82 |
| MsG0080048100.01.T04 | P93295 | 36.957 | 92 | 57 | 1 | 1 | 91 | 8 | 99 | 1.88E-17 | 76.3 |
| MsG0080048100.01.T05 | P93295 | 41.333 | 75 | 43 | 1 | 2 | 75 | 25 | 99 | 3.71E-16 | 72.4 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048100.01.T01 | A0A072VSR5 | 54.198 | 131 | 30 | 1 | 1 | 101 | 1 | 131 | 6.00e-40 | 145 |
| MsG0080048100.01.T02 | A4PSF3 | 86.620 | 142 | 19 | 0 | 1 | 142 | 144 | 285 | 4.59e-80 | 253 |
| MsG0080048100.01.T03 | A4PSF3 | 90.566 | 106 | 10 | 0 | 1 | 106 | 180 | 285 | 1.46e-61 | 203 |
| MsG0080048100.01.T04 | A4PSF3 | 90.323 | 93 | 9 | 0 | 1 | 93 | 193 | 285 | 3.75e-53 | 181 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048100.01 | MsG0580024908.01 | 0.829572 | 4.774434e-55 | 4.905564e-52 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048100.01.T05 | MTR_1g046130 | 77.333 | 75 | 17 | 0 | 1 | 75 | 1 | 75 | 8.18e-39 | 134 |
| MsG0080048100.01.T05 | MTR_6g464340 | 67.857 | 56 | 18 | 0 | 1 | 56 | 36 | 91 | 4.93e-21 | 82.8 |
| MsG0080048100.01.T05 | MTR_8g006720 | 55.738 | 61 | 26 | 1 | 1 | 60 | 66 | 126 | 3.14e-20 | 82.8 |
| MsG0080048100.01.T05 | MTR_1g096730 | 42.308 | 78 | 44 | 1 | 1 | 77 | 22 | 99 | 3.41e-16 | 70.5 |
| MsG0080048100.01.T05 | MTR_4g094272 | 50.000 | 50 | 25 | 0 | 22 | 71 | 2 | 51 | 1.50e-14 | 65.5 |
| MsG0080048100.01.T05 | MTR_8g028510 | 36.620 | 71 | 29 | 1 | 1 | 71 | 140 | 194 | 2.47e-12 | 61.2 |
| MsG0080048100.01.T05 | MTR_7g056813 | 50.000 | 50 | 24 | 1 | 1 | 49 | 290 | 339 | 6.25e-12 | 60.8 |
| MsG0080048100.01.T05 | MTR_7g451250 | 54.167 | 48 | 22 | 0 | 28 | 75 | 3 | 50 | 7.87e-12 | 59.7 |
| MsG0080048100.01.T05 | MTR_2g079840 | 55.814 | 43 | 19 | 0 | 33 | 75 | 1 | 43 | 9.13e-12 | 60.5 |
| MsG0080048100.01.T05 | MTR_0015s0120 | 55.263 | 38 | 17 | 0 | 39 | 76 | 136 | 173 | 5.01e-11 | 58.5 |
| MsG0080048100.01.T04 | MTR_1g046130 | 77.333 | 75 | 17 | 0 | 17 | 91 | 1 | 75 | 6.66e-39 | 135 |
| MsG0080048100.01.T04 | MTR_6g464340 | 69.444 | 72 | 22 | 0 | 1 | 72 | 20 | 91 | 8.02e-31 | 108 |
| MsG0080048100.01.T04 | MTR_8g006720 | 58.442 | 77 | 31 | 1 | 1 | 76 | 50 | 126 | 1.13e-27 | 103 |
| MsG0080048100.01.T04 | MTR_1g096730 | 42.553 | 94 | 53 | 1 | 1 | 93 | 6 | 99 | 1.31e-21 | 85.1 |
| MsG0080048100.01.T04 | MTR_8g028510 | 36.782 | 87 | 39 | 1 | 1 | 87 | 124 | 194 | 5.94e-18 | 76.6 |
| MsG0080048100.01.T04 | MTR_4g094272 | 50.000 | 50 | 25 | 0 | 38 | 87 | 2 | 51 | 2.53e-14 | 65.5 |
| MsG0080048100.01.T04 | MTR_2g021248 | 42.857 | 63 | 36 | 0 | 1 | 63 | 242 | 304 | 6.01e-13 | 64.3 |
| MsG0080048100.01.T04 | MTR_1g069475 | 44.681 | 94 | 48 | 2 | 1 | 90 | 1 | 94 | 1.07e-12 | 63.2 |
| MsG0080048100.01.T04 | MTR_3g102690 | 32.941 | 85 | 57 | 0 | 1 | 85 | 72 | 156 | 1.85e-12 | 61.6 |
| MsG0080048100.01.T04 | MTR_7g056813 | 46.970 | 66 | 30 | 2 | 1 | 65 | 278 | 339 | 4.99e-12 | 62.0 |
| MsG0080048100.01.T04 | MTR_0007s0410 | 28.261 | 92 | 66 | 0 | 3 | 94 | 89 | 180 | 8.28e-12 | 60.5 |
| MsG0080048100.01.T04 | MTR_7g451250 | 54.167 | 48 | 22 | 0 | 44 | 91 | 3 | 50 | 1.02e-11 | 59.7 |
| MsG0080048100.01.T04 | MTR_2g079840 | 55.814 | 43 | 19 | 0 | 49 | 91 | 1 | 43 | 1.50e-11 | 60.5 |
| MsG0080048100.01.T04 | MTR_6g033560 | 51.020 | 49 | 23 | 1 | 1 | 48 | 135 | 183 | 3.98e-11 | 59.3 |
| MsG0080048100.01.T03 | MTR_1g046130 | 77.333 | 75 | 17 | 0 | 30 | 104 | 1 | 75 | 4.77e-39 | 135 |
| MsG0080048100.01.T03 | MTR_6g464340 | 70.886 | 79 | 23 | 0 | 7 | 85 | 13 | 91 | 6.09e-36 | 122 |
| MsG0080048100.01.T03 | MTR_8g006720 | 60.000 | 90 | 35 | 1 | 1 | 89 | 37 | 126 | 1.65e-34 | 121 |
| MsG0080048100.01.T03 | MTR_1g096730 | 41.237 | 97 | 56 | 1 | 11 | 106 | 3 | 99 | 5.73e-22 | 86.7 |
| MsG0080048100.01.T03 | MTR_8g028510 | 36.634 | 101 | 48 | 1 | 4 | 104 | 114 | 198 | 1.04e-20 | 84.3 |
| MsG0080048100.01.T03 | MTR_2g021248 | 44.737 | 76 | 42 | 0 | 1 | 76 | 229 | 304 | 1.64e-18 | 79.7 |
| MsG0080048100.01.T03 | MTR_7g056813 | 48.101 | 79 | 36 | 2 | 1 | 78 | 265 | 339 | 2.68e-16 | 74.3 |
| MsG0080048100.01.T03 | MTR_3g102690 | 34.409 | 93 | 61 | 0 | 6 | 98 | 64 | 156 | 4.95e-15 | 68.9 |
| MsG0080048100.01.T03 | MTR_4g094272 | 50.000 | 50 | 25 | 0 | 51 | 100 | 2 | 51 | 3.15e-14 | 65.5 |
| MsG0080048100.01.T03 | MTR_1g054775 | 31.633 | 98 | 67 | 0 | 1 | 98 | 1081 | 1178 | 9.48e-14 | 67.4 |
| MsG0080048100.01.T03 | MTR_6g033560 | 50.943 | 53 | 25 | 1 | 10 | 61 | 131 | 183 | 3.20e-13 | 65.9 |
| MsG0080048100.01.T03 | MTR_6g006375 | 34.375 | 96 | 63 | 0 | 3 | 98 | 83 | 178 | 1.44e-12 | 63.2 |
| MsG0080048100.01.T03 | MTR_1g069475 | 44.681 | 94 | 48 | 2 | 14 | 103 | 1 | 94 | 1.73e-12 | 62.8 |
| MsG0080048100.01.T03 | MTR_0007s0410 | 28.261 | 92 | 66 | 0 | 16 | 107 | 89 | 180 | 1.09e-11 | 60.5 |
| MsG0080048100.01.T03 | MTR_7g451250 | 54.167 | 48 | 22 | 0 | 57 | 104 | 3 | 50 | 1.34e-11 | 60.1 |
| MsG0080048100.01.T03 | MTR_2g079840 | 55.814 | 43 | 19 | 0 | 62 | 104 | 1 | 43 | 1.54e-11 | 60.8 |
| MsG0080048100.01.T03 | MTR_0061s0090 | 29.293 | 99 | 69 | 1 | 1 | 98 | 202 | 300 | 7.18e-11 | 58.9 |
| MsG0080048100.01.T03 | MTR_7g104745 | 31.683 | 101 | 66 | 2 | 4 | 104 | 618 | 715 | 9.46e-11 | 58.9 |
| MsG0080048100.01.T02 | MTR_8g006720 | 57.937 | 126 | 52 | 1 | 1 | 125 | 1 | 126 | 2.66e-50 | 163 |
| MsG0080048100.01.T02 | MTR_1g046130 | 77.333 | 75 | 17 | 0 | 66 | 140 | 1 | 75 | 1.68e-38 | 135 |
| MsG0080048100.01.T02 | MTR_6g464340 | 70.886 | 79 | 23 | 0 | 43 | 121 | 13 | 91 | 2.25e-35 | 122 |
| MsG0080048100.01.T02 | MTR_8g028510 | 36.232 | 138 | 54 | 2 | 3 | 140 | 95 | 198 | 5.47e-27 | 102 |
| MsG0080048100.01.T02 | MTR_7g056813 | 45.536 | 112 | 56 | 2 | 4 | 114 | 232 | 339 | 5.08e-26 | 102 |
| MsG0080048100.01.T02 | MTR_1g096730 | 41.237 | 97 | 56 | 1 | 47 | 142 | 3 | 99 | 3.30e-21 | 85.9 |
| MsG0080048100.01.T02 | MTR_2g021248 | 44.304 | 79 | 44 | 0 | 34 | 112 | 226 | 304 | 4.72e-19 | 82.8 |
| MsG0080048100.01.T02 | MTR_6g033560 | 39.796 | 98 | 40 | 2 | 1 | 97 | 104 | 183 | 1.16e-18 | 82.4 |
| MsG0080048100.01.T02 | MTR_1g054775 | 31.061 | 132 | 91 | 0 | 3 | 134 | 1047 | 1178 | 2.12e-18 | 82.4 |
| MsG0080048100.01.T02 | MTR_4g008360 | 44.706 | 85 | 46 | 1 | 4 | 87 | 57 | 141 | 1.42e-17 | 78.2 |
| MsG0080048100.01.T02 | MTR_8g068370 | 46.753 | 77 | 41 | 0 | 1 | 77 | 118 | 194 | 1.84e-17 | 78.2 |
| MsG0080048100.01.T02 | MTR_3g102690 | 30.000 | 120 | 84 | 0 | 15 | 134 | 37 | 156 | 9.62e-17 | 74.3 |
| MsG0080048100.01.T02 | MTR_6g006375 | 32.787 | 122 | 82 | 0 | 13 | 134 | 57 | 178 | 6.51e-15 | 70.5 |
| MsG0080048100.01.T02 | MTR_7g111367 | 30.000 | 110 | 76 | 1 | 1 | 110 | 90 | 198 | 2.27e-14 | 70.1 |
| MsG0080048100.01.T02 | MTR_4g094272 | 50.000 | 50 | 25 | 0 | 87 | 136 | 2 | 51 | 6.82e-14 | 65.9 |
| MsG0080048100.01.T02 | MTR_2g064455 | 31.579 | 133 | 75 | 2 | 3 | 134 | 48 | 165 | 7.82e-13 | 64.7 |
| MsG0080048100.01.T02 | MTR_0061s0090 | 30.252 | 119 | 82 | 1 | 17 | 134 | 182 | 300 | 9.14e-13 | 65.5 |
| MsG0080048100.01.T02 | MTR_1g069475 | 44.681 | 94 | 48 | 2 | 50 | 139 | 1 | 94 | 3.75e-12 | 63.2 |
| MsG0080048100.01.T02 | MTR_1g026690 | 30.702 | 114 | 78 | 1 | 19 | 132 | 81 | 193 | 3.37e-11 | 61.2 |
| MsG0080048100.01.T02 | MTR_2g079840 | 55.814 | 43 | 19 | 0 | 98 | 140 | 1 | 43 | 3.43e-11 | 60.8 |
| MsG0080048100.01.T02 | MTR_0007s0410 | 28.261 | 92 | 66 | 0 | 52 | 143 | 89 | 180 | 3.67e-11 | 60.1 |
| MsG0080048100.01.T02 | MTR_7g451250 | 54.167 | 48 | 22 | 0 | 93 | 140 | 3 | 50 | 4.44e-11 | 59.7 |
| MsG0080048100.01.T02 | MTR_8g442690 | 25.714 | 140 | 100 | 1 | 2 | 137 | 347 | 486 | 5.69e-11 | 60.5 |
| MsG0080048100.01.T02 | MTR_2g090750 | 31.092 | 119 | 82 | 0 | 15 | 133 | 330 | 448 | 5.70e-11 | 60.8 |
| MsG0080048100.01.T02 | MTR_2g437490 | 26.087 | 115 | 85 | 0 | 20 | 134 | 67 | 181 | 7.80e-11 | 58.9 |
| MsG0080048100.01.T01 | MTR_1g046130 | 54.198 | 131 | 30 | 1 | 1 | 101 | 1 | 131 | 1.52e-43 | 145 |
| MsG0080048100.01.T01 | MTR_6g464340 | 63.265 | 49 | 18 | 0 | 1 | 49 | 36 | 84 | 2.09e-15 | 67.8 |
| MsG0080048100.01.T01 | MTR_8g006720 | 54.000 | 50 | 22 | 1 | 1 | 49 | 66 | 115 | 6.41e-14 | 65.5 |
| MsG0080048100.01.T01 | MTR_7g056813 | 43.548 | 62 | 34 | 1 | 1 | 61 | 290 | 351 | 3.07e-12 | 61.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048100.01.T05 | AT4G29090 | 47.297 | 74 | 39 | 0 | 1 | 74 | 24 | 97 | 1.12e-19 | 83.2 |
| MsG0080048100.01.T04 | AT4G29090 | 45.556 | 90 | 49 | 0 | 1 | 90 | 8 | 97 | 1.50e-23 | 95.1 |
| MsG0080048100.01.T03 | AT4G29090 | 44.792 | 96 | 53 | 0 | 8 | 103 | 2 | 97 | 4.45e-26 | 102 |
| MsG0080048100.01.T02 | AT4G29090 | 44.792 | 96 | 53 | 0 | 44 | 139 | 2 | 97 | 9.53e-26 | 103 |
| MsG0080048100.01.T02 | AT3G24255 | 29.851 | 134 | 93 | 1 | 15 | 148 | 101 | 233 | 5.31e-13 | 66.6 |
| MsG0080048100.01.T02 | AT3G24255 | 29.851 | 134 | 93 | 1 | 15 | 148 | 101 | 233 | 9.20e-12 | 63.2 |
| MsG0080048100.01.T02 | AT3G24255 | 29.851 | 134 | 93 | 1 | 15 | 148 | 101 | 233 | 9.20e-12 | 63.2 |
| MsG0080048100.01.T02 | AT3G24255 | 29.851 | 134 | 93 | 1 | 15 | 148 | 47 | 179 | 1.12e-11 | 62.8 |
Find 60 sgRNAs with CRISPR-Local
Find 74 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTTTCCCCGGTCTTCCTTT+TGG | 0.228357 | contig207end:-38711 | MsG0080048100.01.T01:intron |
| CCCCATTCCACCAAACTCTT+TGG | 0.247186 | contig207end:+38837 | None:intergenic |
| GCTTTGCTTGGCGGAGTATT+TGG | 0.252750 | contig207end:-38664 | MsG0080048100.01.T01:CDS |
| ATATCATCTATAACTGAATT+TGG | 0.261267 | contig207end:+38946 | None:intergenic |
| CCCATTCCACCAAACTCTTT+GGG | 0.319562 | contig207end:+38838 | None:intergenic |
| ACTCTAGCAACCAGAGATTC+AGG | 0.324847 | contig207end:+38748 | None:intergenic |
| AGTTTGGTGGAATGGGGTTT+CGG | 0.344376 | contig207end:-38831 | MsG0080048100.01.T01:CDS |
| CCCAAAGAGTTTGGTGGAAT+GGG | 0.344793 | contig207end:-38838 | MsG0080048100.01.T01:CDS |
| CATGGTTGTAGGTGGACAAT+TGG | 0.353252 | contig207end:-38620 | MsG0080048100.01.T01:CDS |
| GTAACAACCGAGGGATTAAA+TGG | 0.377108 | contig207end:-38885 | MsG0080048100.01.T01:CDS |
| TTCGAGGTGTTCATGGTAGT+TGG | 0.384976 | contig207end:-38559 | MsG0080048100.01.T01:CDS |
| AGAAGATGATCAATGCATTA+TGG | 0.387031 | contig207end:-38921 | MsG0080048100.01.T02:CDS |
| AAATGCGTGGTAGAGATAAT+AGG | 0.387110 | contig207end:-39113 | MsG0080048100.01.T02:CDS |
| TCTGTCTAAACCTGAATCTC+TGG | 0.401350 | contig207end:-38758 | MsG0080048100.01.T01:intron |
| ATGGCATGCCCCAAAGAGTT+TGG | 0.403066 | contig207end:-38847 | MsG0080048100.01.T01:CDS |
| GACAATTGGGTATGGGCAGC+AGG | 0.409873 | contig207end:-38606 | MsG0080048100.01.T01:CDS |
| CCATGGGTTCGAGGTGTTCA+TGG | 0.424878 | contig207end:-38566 | MsG0080048100.01.T01:CDS |
| CCCCAAAGAGTTTGGTGGAA+TGG | 0.435404 | contig207end:-38839 | MsG0080048100.01.T01:CDS |
| AATGCATTATGGTGGGGAGG+AGG | 0.471722 | contig207end:-38910 | MsG0080048100.01.T01:CDS |
| GTAATAACCCGAGCTTTGCT+TGG | 0.477679 | contig207end:-38676 | MsG0080048100.01.T01:CDS |
| AGCATTATCGAAGGCGGGAA+AGG | 0.479206 | contig207end:-39031 | MsG0080048100.01.T02:CDS |
| ATGGTTGTAGGTGGACAATT+GGG | 0.482469 | contig207end:-38619 | MsG0080048100.01.T01:CDS |
| ATGCCATCCATTTAATCCCT+CGG | 0.483990 | contig207end:+38878 | None:intergenic |
| TTAAATCGAGGTACTTTCCC+CGG | 0.489104 | contig207end:-38723 | MsG0080048100.01.T01:intron |
| CTATGGTAGCTAAGCAAGGA+TGG | 0.505774 | contig207end:-38786 | MsG0080048100.01.T01:intron |
| TAGATACTCATTATATATGA+AGG | 0.506924 | contig207end:+38976 | None:intergenic |
| TGTAGGTGGACAATTGGGTA+TGG | 0.513318 | contig207end:-38614 | MsG0080048100.01.T01:CDS |
| ATGCATTATGGTGGGGAGGA+GGG | 0.515804 | contig207end:-38909 | MsG0080048100.01.T01:CDS |
| ATAACCCGAGCTTTGCTTGG+CGG | 0.515982 | contig207end:-38673 | MsG0080048100.01.T01:CDS |
| TACTCCGCCAAGCAAAGCTC+GGG | 0.521838 | contig207end:+38669 | None:intergenic |
| GAGGGATTAAATGGATGGCA+TGG | 0.528552 | contig207end:-38876 | MsG0080048100.01.T01:CDS |
| ATACTCCGCCAAGCAAAGCT+CGG | 0.529012 | contig207end:+38668 | None:intergenic |
| CCAAAGAGTTTGGTGGAATG+GGG | 0.545452 | contig207end:-38837 | MsG0080048100.01.T01:CDS |
| TCAGCGTGATGAATGATCCA+TGG | 0.549092 | contig207end:-38583 | MsG0080048100.01.T01:CDS |
| GTAGGTGGACAATTGGGTAT+GGG | 0.560039 | contig207end:-38613 | MsG0080048100.01.T01:CDS |
| CAACCGAGGGATTAAATGGA+TGG | 0.565008 | contig207end:-38881 | MsG0080048100.01.T01:CDS |
| CCATGAACACCTCGAACCCA+TGG | 0.565182 | contig207end:+38566 | None:intergenic |
| GATGATCAATGCATTATGGT+GGG | 0.566264 | contig207end:-38917 | MsG0080048100.01.T01:CDS |
| GGACGAGCATTATCGAAGGC+GGG | 0.574345 | contig207end:-39036 | MsG0080048100.01.T02:CDS |
| ATGGATGGCATGGGAACGAA+TGG | 0.574835 | contig207end:-38866 | MsG0080048100.01.T01:CDS |
| GAGACGAATAAATCCGCGGA+GGG | 0.576512 | contig207end:-39058 | MsG0080048100.01.T02:CDS |
| CTCGCTATGGTAGCTAAGCA+AGG | 0.579207 | contig207end:-38790 | MsG0080048100.01.T01:intron |
| GCATGCCCCAAAGAGTTTGG+TGG | 0.580188 | contig207end:-38844 | MsG0080048100.01.T01:CDS |
| CCATTCCACCAAACTCTTTG+GGG | 0.589078 | contig207end:+38839 | None:intergenic |
| CAGCGTGATGAATGATCCAT+GGG | 0.592042 | contig207end:-38582 | MsG0080048100.01.T01:CDS |
| ATGCACATCCTGGCTTTATG+GGG | 0.592546 | contig207end:+38529 | None:intergenic |
| ATGAATGATCCATGGGTTCG+AGG | 0.598097 | contig207end:-38575 | MsG0080048100.01.T01:CDS |
| ATCAATGCATTATGGTGGGG+AGG | 0.602412 | contig207end:-38913 | MsG0080048100.01.T01:CDS |
| AGGGATTAAATGGATGGCAT+GGG | 0.603185 | contig207end:-38875 | MsG0080048100.01.T01:CDS |
| AGATGATCAATGCATTATGG+TGG | 0.603211 | contig207end:-38918 | MsG0080048100.01.T01:CDS |
| CTATAACTGAATTTGGCAGA+AGG | 0.618276 | contig207end:+38953 | None:intergenic |
| GAGGGGACGAGCATTATCGA+AGG | 0.654024 | contig207end:-39040 | MsG0080048100.01.T02:CDS |
| GATAATGCTCGTCCCCTCCG+CGG | 0.661747 | contig207end:+39045 | None:intergenic |
| GGGACGAGCATTATCGAAGG+CGG | 0.674228 | contig207end:-39037 | MsG0080048100.01.T02:CDS |
| ATGATCAATGCATTATGGTG+GGG | 0.676870 | contig207end:-38916 | MsG0080048100.01.T01:CDS |
| GAGGAGGGAGTAACAACCGA+GGG | 0.691463 | contig207end:-38894 | MsG0080048100.01.T01:CDS |
| AGACGAATAAATCCGCGGAG+GGG | 0.696517 | contig207end:-39057 | MsG0080048100.01.T02:CDS |
| GGAGACGAATAAATCCGCGG+AGG | 0.722082 | contig207end:-39059 | MsG0080048100.01.T02:CDS |
| TATGGAGACGAATAAATCCG+CGG | 0.742259 | contig207end:-39062 | MsG0080048100.01.T02:CDS |
| GGAGGAGGGAGTAACAACCG+AGG | 0.760951 | contig207end:-38895 | MsG0080048100.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATATCATCTATAACTGAATT+TGG | + | contig207end:38712-38731 | None:intergenic | 20.0% |
| !! | ATCCTTGATAAAAGAAAAAA+TGG | + | contig207end:38569-38588 | None:intergenic | 20.0% |
| !! | TAGATACTCATTATATATGA+AGG | + | contig207end:38682-38701 | None:intergenic | 20.0% |
| !!! | GTCCATTTTTTCTTTTATCA+AGG | - | contig207end:38564-38583 | MsG0080048100.01.T01:CDS | 25.0% |
| AGAAGATGATCAATGCATTA+TGG | - | contig207end:38734-38753 | MsG0080048100.01.T01:intron | 30.0% | |
| ! | CTTTTATCAAGGATCGTATA+TGG | - | contig207end:38575-38594 | MsG0080048100.01.T01:CDS | 30.0% |
| ! | TTGCTAGAGTTTTTAAATCG+AGG | - | contig207end:38920-38939 | MsG0080048100.01.T02:CDS | 30.0% |
| !!! | AGTTACTTTTACATGGTTGT+AGG | - | contig207end:39024-39043 | MsG0080048100.01.T02:CDS | 30.0% |
| AGATGATCAATGCATTATGG+TGG | - | contig207end:38737-38756 | MsG0080048100.01.T01:intron | 35.0% | |
| ATGATCAATGCATTATGGTG+GGG | - | contig207end:38739-38758 | MsG0080048100.01.T01:intron | 35.0% | |
| CTATAACTGAATTTGGCAGA+AGG | + | contig207end:38705-38724 | None:intergenic | 35.0% | |
| GATGATCAATGCATTATGGT+GGG | - | contig207end:38738-38757 | MsG0080048100.01.T01:intron | 35.0% | |
| ! | CAAAGCTTTTAATCTCGCTA+TGG | - | contig207end:38852-38871 | MsG0080048100.01.T01:CDS | 35.0% |
| ! | TACTTTTACATGGTTGTAGG+TGG | - | contig207end:39027-39046 | MsG0080048100.01.T02:CDS | 35.0% |
| AGGGATTAAATGGATGGCAT+GGG | - | contig207end:38780-38799 | MsG0080048100.01.T01:intron | 40.0% | |
| ATGCCATCCATTTAATCCCT+CGG | + | contig207end:38780-38799 | None:intergenic | 40.0% | |
| ATGGTTGTAGGTGGACAATT+GGG | - | contig207end:39036-39055 | MsG0080048100.01.T02:CDS | 40.0% | |
| GTAACAACCGAGGGATTAAA+TGG | - | contig207end:38770-38789 | MsG0080048100.01.T01:intron | 40.0% | |
| TATGGAGACGAATAAATCCG+CGG | - | contig207end:38593-38612 | MsG0080048100.01.T01:CDS | 40.0% | |
| TCTGTCTAAACCTGAATCTC+TGG | - | contig207end:38897-38916 | MsG0080048100.01.T01:CDS | 40.0% | |
| ! | TTAAATCGAGGTACTTTCCC+CGG | - | contig207end:38932-38951 | MsG0080048100.01.T02:CDS | 40.0% |
| !!! | TCGCGTCAGTTACTTTTACA+TGG | - | contig207end:39017-39036 | MsG0080048100.01.T02:CDS | 40.0% |
| ACTCTAGCAACCAGAGATTC+AGG | + | contig207end:38910-38929 | None:intergenic | 45.0% | |
| ATGAATGATCCATGGGTTCG+AGG | - | contig207end:39080-39099 | MsG0080048100.01.T02:CDS | 45.0% | |
| CAACCGAGGGATTAAATGGA+TGG | - | contig207end:38774-38793 | MsG0080048100.01.T01:intron | 45.0% | |
| CAGCGTGATGAATGATCCAT+GGG | - | contig207end:39073-39092 | MsG0080048100.01.T02:CDS | 45.0% | |
| CATGGTTGTAGGTGGACAAT+TGG | - | contig207end:39035-39054 | MsG0080048100.01.T02:CDS | 45.0% | |
| CCATTCCACCAAACTCTTTG+GGG | + | contig207end:38819-38838 | None:intergenic | 45.0% | |
| CCCATTCCACCAAACTCTTT+GGG | + | contig207end:38820-38839 | None:intergenic | 45.0% | |
| CTATGGTAGCTAAGCAAGGA+TGG | - | contig207end:38869-38888 | MsG0080048100.01.T01:CDS | 45.0% | |
| GAGGGATTAAATGGATGGCA+TGG | - | contig207end:38779-38798 | MsG0080048100.01.T01:intron | 45.0% | |
| GTAATAACCCGAGCTTTGCT+TGG | - | contig207end:38979-38998 | MsG0080048100.01.T02:CDS | 45.0% | |
| GTAGGTGGACAATTGGGTAT+GGG | - | contig207end:39042-39061 | MsG0080048100.01.T02:CDS | 45.0% | |
| TCAGCGTGATGAATGATCCA+TGG | - | contig207end:39072-39091 | MsG0080048100.01.T02:CDS | 45.0% | |
| TGTAGGTGGACAATTGGGTA+TGG | - | contig207end:39041-39060 | MsG0080048100.01.T02:CDS | 45.0% | |
| ! | AGTTTGGTGGAATGGGGTTT+CGG | - | contig207end:38824-38843 | MsG0080048100.01.T01:CDS | 45.0% |
| ! | ATCAATGCATTATGGTGGGG+AGG | - | contig207end:38742-38761 | MsG0080048100.01.T01:intron | 45.0% |
| ! | CCAAAGAGTTTGGTGGAATG+GGG | - | contig207end:38818-38837 | MsG0080048100.01.T01:CDS | 45.0% |
| ! | CCCAAAGAGTTTGGTGGAAT+GGG | - | contig207end:38817-38836 | MsG0080048100.01.T01:CDS | 45.0% |
| ! | TTCGAGGTGTTCATGGTAGT+TGG | - | contig207end:39096-39115 | MsG0080048100.01.T02:CDS | 45.0% |
| !! | TTCCTTTTGGGGGCTAAACT+CGG | - | contig207end:38957-38976 | MsG0080048100.01.T02:CDS | 45.0% |
| AGACGAATAAATCCGCGGAG+GGG | - | contig207end:38598-38617 | MsG0080048100.01.T01:CDS | 50.0% | |
| AGCATTATCGAAGGCGGGAA+AGG | - | contig207end:38624-38643 | MsG0080048100.01.T01:CDS | 50.0% | |
| ATAACCCGAGCTTTGCTTGG+CGG | - | contig207end:38982-39001 | MsG0080048100.01.T02:CDS | 50.0% | |
| ATACTCCGCCAAGCAAAGCT+CGG | + | contig207end:38990-39009 | None:intergenic | 50.0% | |
| ATGGATGGCATGGGAACGAA+TGG | - | contig207end:38789-38808 | MsG0080048100.01.T01:intron | 50.0% | |
| ATGGCATGCCCCAAAGAGTT+TGG | - | contig207end:38808-38827 | MsG0080048100.01.T01:CDS | 50.0% | |
| CCCCATTCCACCAAACTCTT+TGG | + | contig207end:38821-38840 | None:intergenic | 50.0% | |
| CTCGCTATGGTAGCTAAGCA+AGG | - | contig207end:38865-38884 | MsG0080048100.01.T01:CDS | 50.0% | |
| GAGACGAATAAATCCGCGGA+GGG | - | contig207end:38597-38616 | MsG0080048100.01.T01:CDS | 50.0% | |
| TACCGAGTTTAGCCCCCAAA+AGG | + | contig207end:38962-38981 | None:intergenic | 50.0% | |
| TTAGCCCCCAAAAGGAAGAC+CGG | + | contig207end:38954-38973 | None:intergenic | 50.0% | |
| ! | AATGCATTATGGTGGGGAGG+AGG | - | contig207end:38745-38764 | MsG0080048100.01.T01:intron | 50.0% |
| ! | ACTTTCCCCGGTCTTCCTTT+TGG | - | contig207end:38944-38963 | MsG0080048100.01.T02:CDS | 50.0% |
| ! | ATGCATTATGGTGGGGAGGA+GGG | - | contig207end:38746-38765 | MsG0080048100.01.T01:intron | 50.0% |
| ! | CCCCAAAGAGTTTGGTGGAA+TGG | - | contig207end:38816-38835 | MsG0080048100.01.T01:CDS | 50.0% |
| ! | CTTTCCCCGGTCTTCCTTTT+GGG | - | contig207end:38945-38964 | MsG0080048100.01.T02:CDS | 50.0% |
| ! | GCTTTGCTTGGCGGAGTATT+TGG | - | contig207end:38991-39010 | MsG0080048100.01.T02:CDS | 50.0% |
| ! | TTTCCCCGGTCTTCCTTTTG+GGG | - | contig207end:38946-38965 | MsG0080048100.01.T02:CDS | 50.0% |
| CCATGAACACCTCGAACCCA+TGG | + | contig207end:39092-39111 | None:intergenic | 55.0% | |
| GACAATTGGGTATGGGCAGC+AGG | - | contig207end:39049-39068 | MsG0080048100.01.T02:CDS | 55.0% | |
| GAGGAGGGAGTAACAACCGA+GGG | - | contig207end:38761-38780 | MsG0080048100.01.T01:intron | 55.0% | |
| GAGGGGACGAGCATTATCGA+AGG | - | contig207end:38615-38634 | MsG0080048100.01.T01:CDS | 55.0% | |
| GCATGCCCCAAAGAGTTTGG+TGG | - | contig207end:38811-38830 | MsG0080048100.01.T01:CDS | 55.0% | |
| GGACGAGCATTATCGAAGGC+GGG | - | contig207end:38619-38638 | MsG0080048100.01.T01:CDS | 55.0% | |
| GGAGACGAATAAATCCGCGG+AGG | - | contig207end:38596-38615 | MsG0080048100.01.T01:CDS | 55.0% | |
| GGGACGAGCATTATCGAAGG+CGG | - | contig207end:38618-38637 | MsG0080048100.01.T01:CDS | 55.0% | |
| TACTCCGCCAAGCAAAGCTC+GGG | + | contig207end:38989-39008 | None:intergenic | 55.0% | |
| TAGCCCCCAAAAGGAAGACC+GGG | + | contig207end:38953-38972 | None:intergenic | 55.0% | |
| ! | CCATGGGTTCGAGGTGTTCA+TGG | - | contig207end:39089-39108 | MsG0080048100.01.T02:CDS | 55.0% |
| ! | TTCCCCGGTCTTCCTTTTGG+GGG | - | contig207end:38947-38966 | MsG0080048100.01.T02:CDS | 55.0% |
| AGCCCCCAAAAGGAAGACCG+GGG | + | contig207end:38952-38971 | None:intergenic | 60.0% | |
| GATAATGCTCGTCCCCTCCG+CGG | + | contig207end:38613-38632 | None:intergenic | 60.0% | |
| GGAGGAGGGAGTAACAACCG+AGG | - | contig207end:38760-38779 | MsG0080048100.01.T01:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| contig207end | gene | 38544 | 39133 | 38544 | ID=MsG0080048100.01;Name=MsG0080048100.01 |
| contig207end | mRNA | 38544 | 38938 | 38544 | ID=MsG0080048100.01.T01;Parent=MsG0080048100.01;Name=MsG0080048100.01.T01;_AED=0.42;_eAED=0.42;_QI=0|0|0|1|0|0|2|0|101 |
| contig207end | exon | 38544 | 38710 | 38544 | ID=MsG0080048100.01.T01:exon:2030;Parent=MsG0080048100.01.T01 |
| contig207end | exon | 38800 | 38938 | 38800 | ID=MsG0080048100.01.T01:exon:2029;Parent=MsG0080048100.01.T01 |
| contig207end | CDS | 38800 | 38938 | 38800 | ID=MsG0080048100.01.T01:cds;Parent=MsG0080048100.01.T01 |
| contig207end | CDS | 38544 | 38710 | 38544 | ID=MsG0080048100.01.T01:cds;Parent=MsG0080048100.01.T01 |
| contig207end | mRNA | 38597 | 39133 | 38597 | ID=MsG0080048100.01.T02;Parent=MsG0080048100.01;Name=MsG0080048100.01.T02;_AED=0.35;_eAED=0.47;_QI=0|-1|0|1|-1|1|1|0|178 |
| contig207end | exon | 38597 | 39133 | 38597 | ID=MsG0080048100.01.T02:exon:2031;Parent=MsG0080048100.01.T02 |
| contig207end | CDS | 38597 | 39133 | 38597 | ID=MsG0080048100.01.T02:cds;Parent=MsG0080048100.01.T02 |
| contig207end | mRNA | 38597 | 39025 | 38597 | ID=MsG0080048100.01.T03;Parent=MsG0080048100.01;Name=MsG0080048100.01.T03;_AED=0.38;_eAED=0.52;_QI=0|-1|0|1|-1|0|1|0|142 |
| contig207end | exon | 38597 | 39025 | 38597 | ID=MsG0080048100.01.T03:exon:2032;Parent=MsG0080048100.01.T03 |
| contig207end | CDS | 38597 | 39025 | 38597 | ID=MsG0080048100.01.T03:cds;Parent=MsG0080048100.01.T03 |
| contig207end | mRNA | 38597 | 38986 | 38597 | ID=MsG0080048100.01.T04;Parent=MsG0080048100.01;Name=MsG0080048100.01.T04;_AED=0.39;_eAED=0.54;_QI=0|-1|0|1|-1|0|1|0|129 |
| contig207end | exon | 38597 | 38986 | 38597 | ID=MsG0080048100.01.T04:exon:2033;Parent=MsG0080048100.01.T04 |
| contig207end | CDS | 38597 | 38986 | 38597 | ID=MsG0080048100.01.T04:cds;Parent=MsG0080048100.01.T04 |
| contig207end | mRNA | 38597 | 38938 | 38597 | ID=MsG0080048100.01.T05;Parent=MsG0080048100.01;Name=MsG0080048100.01.T05;_AED=0.41;_eAED=0.57;_QI=0|-1|0|1|-1|0|1|0|113 |
| contig207end | exon | 38597 | 38938 | 38597 | ID=MsG0080048100.01.T05:exon:2034;Parent=MsG0080048100.01.T05 |
| contig207end | CDS | 38597 | 38938 | 38597 | ID=MsG0080048100.01.T05:cds;Parent=MsG0080048100.01.T05 |
| Gene Sequence |
| Protein sequence |
