AlfalfaGEDB | MsG0580024908.01

M. sativa cultivar ZhongmuNo.1 / MsG0580024908.01

KEGG: K02994
GO: GO:0003735 GO:0005840 GO:0006412
PFAM: PF00410

Nr:

Query id	Subject id	identity %	alignment length	mis matches	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024908.01.T01	YP_010173611.1	83.544	79	13	5	83	56	134	1.01E-38	135
MsG0580024908.01.T03	QJD21000.1	96.875	32	1	1	32	86	117	3.57E-12	65.1
MsG0580024908.01.T02	YP_010173611.1	83.544	79	13	1	79	56	134	1.28E-38	135

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024908.01.T01	A4GGE0	78.481	79	17	5	83	56	134	1.39E-38	127
MsG0580024908.01.T02	A4GGE0	78.481	79	17	1	79	56	134	1.71E-38	127
MsG0580024908.01.T03	Q2PMQ0	96.875	32	1	1	32	103	134	6.98E-15	65.1

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024908.01.T02	A0A898N911	83.544	79	13	0	1	79	56	134	6.13e-39	135
MsG0580024908.01.T03	A0A6M3REF7	96.875	32	1	0	1	32	86	117	1.71e-12	65.1

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048100.01	MsG0580024908.01	0.829572	4.774434e-55	4.905564e-52
MsG0180001758.01	MsG0580024908.01	0.839942	1.171233e-57	1.642944e-54
MsG0380013922.01	MsG0580024908.01	0.851638	7.789481e-61	1.586763e-57
MsG0580024908.01	MsG0580028022.01	0.814355	1.607003e-51	1.075866e-48
MsG0580024908.01	MsG0580029409.01	0.830529	2.790317e-55	2.947496e-52
MsG0580024908.01	MsG0680031180.01	0.879664	1.036021e-69	5.665162e-66
MsG0580024908.01	MsG0680033712.01	0.843499	1.349321e-58	2.115172e-55
MsG0580024908.01	MsG0680034123.01	0.863791	1.934818e-64	5.939254e-61
MsG0580024908.01	MsG0680035251.01	0.815612	8.456982e-52	5.859040e-49
MsG0580024908.01	MsG0780035997.01	0.874668	5.639657e-68	2.560195e-64
MsG0580024908.01	MsG0780036460.01	0.830596	2.686008e-55	2.843080e-52
MsG0580024908.01	MsG0780037869.01	0.819069	1.409295e-52	1.073458e-49
MsG0380015521.01	MsG0580024908.01	0.839019	2.034830e-57	2.774084e-54
MsG0380015522.01	MsG0580024908.01	0.899512	1.841259e-77	2.284694e-73
MsG0380016059.01	MsG0580024908.01	0.911465	5.895042e-83	1.264989e-78

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024908.01.T01	MTR_4g059910	84.211	76	12	7	82	66	141	1.43e-39	127
MsG0580024908.01.T01	MTR_4g034810	84.058	69	11	7	75	50	118	4.09e-34	114
MsG0580024908.01.T01	MTR_3g035490	75.676	37	9	7	43	165	201	2.87e-11	57.0
MsG0580024908.01.T03	MTR_4g059910	96.774	31	1	1	31	111	141	2.53e-14	61.2
MsG0580024908.01.T02	MTR_4g059910	84.211	76	12	3	78	66	141	1.88e-39	127
MsG0580024908.01.T02	MTR_4g034810	84.058	69	11	3	71	50	118	6.07e-34	114
MsG0580024908.01.T02	MTR_3g035490	75.676	37	9	3	39	165	201	3.05e-11	56.6

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MsG0580024908.01.T01	ATCG00770	71.429	77	22	7	83	58	134	2.15e-35	117
MsG0580024908.01.T03	ATCG00770	84.375	32	5	1	32	103	134	1.10e-13	59.7
MsG0580024908.01.T02	ATCG00770	71.429	77	22	3	79	58	134	2.62e-35	116

Find 8 sgRNAs with CRISPR-Local

Find 16 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTGTAATTCTTTCTACTTCT+AGG	0.233849	5:+11520291	MsG0580024908.01.T01:CDS
TGTAATTCTTTCTACTTCTA+GGG	0.394272	5:+11520292	MsG0580024908.01.T01:CDS
CTCTACGGTATAGAAGAAAT+AGG	0.398659	5:+11520162	MsG0580024908.01.T01:CDS
CCCATTCCTCTTAAAACTCT+CGG	0.450671	5:-11520266	None:intergenic
GAGGCTCGATTAGAAAGAAT+CGG	0.553245	5:+11520332	MsG0580024908.01.T01:CDS
GTAATTCTTTCTACTTCTAG+GGG	0.588562	5:+11520293	MsG0580024908.01.T01:CDS
GGGTATAATGACACATCGAG+AGG	0.691175	5:+11520313	MsG0580024908.01.T01:CDS
GCTCGATTAGAAAGAATCGG+CGG	0.737146	5:+11520335	MsG0580024908.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	ACTTTTTAGTTTTAACTCTA+CGG	+	Chr5:11520147-11520166	MsG0580024908.01.T01:CDS	20.0%
!	TGTAATTCTTTCTACTTCTA+GGG	+	Chr5:11520292-11520311	MsG0580024908.01.T01:CDS	25.0%
!	TTGTAATTCTTTCTACTTCT+AGG	+	Chr5:11520291-11520310	MsG0580024908.01.T01:CDS	25.0%
!	TTTAAAAAGGATCAGTACAT+TGG	+	Chr5:11520212-11520231	MsG0580024908.01.T01:CDS	25.0%
!!!	GCGAAGTTTTATGTTATATA+TGG	+	Chr5:11520357-11520376	MsG0580024908.01.T01:CDS	25.0%
	GTAATTCTTTCTACTTCTAG+GGG	+	Chr5:11520293-11520312	MsG0580024908.01.T01:CDS	30.0%
	CTCTACGGTATAGAAGAAAT+AGG	+	Chr5:11520162-11520181	MsG0580024908.01.T01:CDS	35.0%
!!!	CAAATTCCGAGAGTTTTAAG+AGG	+	Chr5:11520260-11520279	MsG0580024908.01.T01:CDS	35.0%
	CCCATTCCTCTTAAAACTCT+CGG	-	Chr5:11520269-11520288	None:intergenic	40.0%
	GAGGCTCGATTAGAAAGAAT+CGG	+	Chr5:11520332-11520351	MsG0580024908.01.T01:CDS	40.0%
!!!	CCGAGAGTTTTAAGAGGAAT+GGG	+	Chr5:11520266-11520285	MsG0580024908.01.T01:CDS	40.0%
!!!	CGAGAGTTTTAAGAGGAATG+GGG	+	Chr5:11520267-11520286	MsG0580024908.01.T01:CDS	40.0%
!!!	TCCGAGAGTTTTAAGAGGAA+TGG	+	Chr5:11520265-11520284	MsG0580024908.01.T01:CDS	40.0%
	GCTCGATTAGAAAGAATCGG+CGG	+	Chr5:11520335-11520354	MsG0580024908.01.T01:CDS	45.0%
	GGGTATAATGACACATCGAG+AGG	+	Chr5:11520313-11520332	MsG0580024908.01.T01:CDS	45.0%
!!!	AACTTTTTTTAAATTTAAAA+AGG	+	Chr5:11520199-11520218	MsG0580024908.01.T01:CDS	5.0%

Chromosome	Type	Strat	End	Strand	Name
Chr5	gene	11520131	11520382	11520131	ID=MsG0580024908.01;Name=MsG0580024908.01
Chr5	mRNA	11520131	11520382	11520131	ID=MsG0580024908.01.T01;Parent=MsG0580024908.01;Name=MsG0580024908.01.T01;_AED=0.47;_eAED=0.48;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|83
Chr5	exon	11520131	11520382	11520131	ID=MsG0580024908.01.T01:exon:531;Parent=MsG0580024908.01.T01
Chr5	CDS	11520131	11520382	11520131	ID=MsG0580024908.01.T01:cds;Parent=MsG0580024908.01.T01
Chr5	mRNA	11520143	11520382	11520143	ID=MsG0580024908.01.T02;Parent=MsG0580024908.01;Name=MsG0580024908.01.T02;_AED=0.47;_eAED=0.48;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|79
Chr5	exon	11520143	11520382	11520143	ID=MsG0580024908.01.T02:exon:532;Parent=MsG0580024908.01.T02
Chr5	CDS	11520143	11520382	11520143	ID=MsG0580024908.01.T02:cds;Parent=MsG0580024908.01.T02
Chr5	mRNA	11520284	11520382	11520284	ID=MsG0580024908.01.T03;Parent=MsG0580024908.01;Name=MsG0580024908.01.T03;_AED=0.49;_eAED=0.49;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|32
Chr5	exon	11520284	11520382	11520284	ID=MsG0580024908.01.T03:exon:533;Parent=MsG0580024908.01.T03
Chr5	CDS	11520284	11520382	11520284	ID=MsG0580024908.01.T03:cds;Parent=MsG0580024908.01.T03
Chr5	mRNA	11520320	11520382	11520320	ID=MsG0580024908.01.T04;Parent=MsG0580024908.01;Name=MsG0580024908.01.T04;_AED=0.49;_eAED=0.49;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|20
Chr5	exon	11520320	11520382	11520320	ID=MsG0580024908.01.T04:exon:534;Parent=MsG0580024908.01.T04
Chr5	CDS	11520320	11520382	11520320	ID=MsG0580024908.01.T04:cds;Parent=MsG0580024908.01.T04

Gene Sequence

>MsG0580024908.01.T01

TTTGTCAAAAAAAGAAACTTTTTAGTTTTAACTCTACGGTATAGAAGAAATAGGAAAGAATCGTATAAAACTTTTTTTAAATTTAAAAAGGATCAGTACATTGGTCTACGAATCTATTCTAACTATAAACAAATTCCGAGAGTTTTAAGAGGAATGGGGATTGTAATTCTTTCTACTTCTAGGGGTATAATGACACATCGAGAGGCTCGATTAGAAAGAATCGGCGGCGAAGTTTTATGTTATATATGGTAA

>MsG0580024908.01.T04

ATGACACATCGAGAGGCTCGATTAGAAAGAATCGGCGGCGAAGTTTTATGTTATATATGGTAA

>MsG0580024908.01.T03

ATGGGGATTGTAATTCTTTCTACTTCTAGGGGTATAATGACACATCGAGAGGCTCGATTAGAAAGAATCGGCGGCGAAGTTTTATGTTATATATGGTAA

>MsG0580024908.01.T02

AGAAACTTTTTAGTTTTAACTCTACGGTATAGAAGAAATAGGAAAGAATCGTATAAAACTTTTTTTAAATTTAAAAAGGATCAGTACATTGGTCTACGAATCTATTCTAACTATAAACAAATTCCGAGAGTTTTAAGAGGAATGGGGATTGTAATTCTTTCTACTTCTAGGGGTATAATGACACATCGAGAGGCTCGATTAGAAAGAATCGGCGGCGAAGTTTTATGTTATATATGGTAA

Protein sequence

>MsG0580024908.01.T01

FVKKRNFLVLTLRYRRNRKESYKTFFKFKKDQYIGLRIYSNYKQIPRVLRGMGIVILSTSRGIMTHREARLERIGGEVLCYIW*

>MsG0580024908.01.T04

MTHREARLERIGGEVLCYIW*

>MsG0580024908.01.T03

MGIVILSTSRGIMTHREARLERIGGEVLCYIW*

>MsG0580024908.01.T02

RNFLVLTLRYRRNRKESYKTFFKFKKDQYIGLRIYSNYKQIPRVLRGMGIVILSTSRGIMTHREARLERIGGEVLCYIW*