Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048265.01.T01 | XP_013456628.1 | 88.406 | 69 | 8 | 0 | 1 | 69 | 110 | 178 | 9.79E-35 | 126 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048265.01.T01 | A0A072ULK7 | 88.406 | 69 | 8 | 0 | 1 | 69 | 110 | 178 | 4.68e-35 | 126 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048265.01 | MsG0080048266.01 | 0.849993 | 2.262688e-60 | 4.367995e-57 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048265.01.T01 | MTR_4g077787 | 88.406 | 69 | 8 | 0 | 1 | 69 | 110 | 178 | 1.19e-38 | 126 |
| MsG0080048265.01.T01 | MTR_4g112310 | 84.932 | 73 | 9 | 2 | 1 | 72 | 60 | 131 | 4.80e-37 | 120 |
| MsG0080048265.01.T01 | MTR_4g111640 | 84.932 | 73 | 9 | 2 | 1 | 72 | 110 | 181 | 2.00e-36 | 120 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 12 sgRNAs with CRISPR-Local
Find 11 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAAATGATCATCAAGGTTT+TGG | 0.254697 | contig259end:-2578 | MsG0080048265.01.T01:CDS |
| TGTGGTGCTGGTGCTGTAAT+TGG | 0.293215 | contig259end:+2547 | None:intergenic |
| CTCTTTACTTTGTCATTCCT+TGG | 0.308362 | contig259end:-2445 | MsG0080048265.01.T01:CDS |
| TACTTCATCAGTGGAGTTAG+TGG | 0.390509 | contig259end:-2619 | MsG0080048265.01.T01:CDS |
| GTGCATGATGTGATAGTAAT+AGG | 0.401733 | contig259end:+2469 | None:intergenic |
| TCATTTGCAGATTGTGGTGC+TGG | 0.425648 | contig259end:+2535 | None:intergenic |
| GCATTAGAATGTTGTGCTAT+TGG | 0.440808 | contig259end:+2505 | None:intergenic |
| GCATTCTCATTTGCAGATTG+TGG | 0.492826 | contig259end:+2529 | None:intergenic |
| AGGCCAGAAAATGATCATCA+AGG | 0.582370 | contig259end:-2584 | MsG0080048265.01.T01:CDS |
| GTTAGTGGTCATTGTACAAG+AGG | 0.592736 | contig259end:-2604 | MsG0080048265.01.T01:CDS |
| TTCAAGCATAAATCATGCCA+AGG | 0.596027 | contig259end:+2428 | None:intergenic |
| GGAATGTTCTACTTCATCAG+TGG | 0.644882 | contig259end:-2628 | MsG0080048265.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | GAAAATGATCATCAAGGTTT+TGG | - | contig259end:2476-2495 | MsG0080048265.01.T01:CDS | 30.0% |
| !! | AAACCTTGATGATCATTTTC+TGG | + | contig259end:2476-2495 | None:intergenic | 30.0% |
| CTCTTTACTTTGTCATTCCT+TGG | - | contig259end:2609-2628 | MsG0080048265.01.T01:CDS | 35.0% | |
| GCATTAGAATGTTGTGCTAT+TGG | + | contig259end:2552-2571 | None:intergenic | 35.0% | |
| GTGCATGATGTGATAGTAAT+AGG | + | contig259end:2588-2607 | None:intergenic | 35.0% | |
| AGGCCAGAAAATGATCATCA+AGG | - | contig259end:2470-2489 | MsG0080048265.01.T01:CDS | 40.0% | |
| GCATTCTCATTTGCAGATTG+TGG | + | contig259end:2528-2547 | None:intergenic | 40.0% | |
| GTTAGTGGTCATTGTACAAG+AGG | - | contig259end:2450-2469 | MsG0080048265.01.T01:CDS | 40.0% | |
| TACTTCATCAGTGGAGTTAG+TGG | - | contig259end:2435-2454 | MsG0080048265.01.T01:CDS | 40.0% | |
| TCATTTGCAGATTGTGGTGC+TGG | + | contig259end:2522-2541 | None:intergenic | 45.0% | |
| !! | TGTGGTGCTGGTGCTGTAAT+TGG | + | contig259end:2510-2529 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| contig259end | gene | 2429 | 2647 | 2429 | ID=MsG0080048265.01;Name=MsG0080048265.01 |
| contig259end | mRNA | 2429 | 2647 | 2429 | ID=MsG0080048265.01.T01;Parent=MsG0080048265.01;Name=MsG0080048265.01.T01;_AED=0.32;_eAED=0.32;_QI=0|-1|0|1|-1|1|1|0|72 |
| contig259end | exon | 2429 | 2647 | 2429 | ID=MsG0080048265.01.T01:exon:21191;Parent=MsG0080048265.01.T01 |
| contig259end | CDS | 2429 | 2647 | 2429 | ID=MsG0080048265.01.T01:cds;Parent=MsG0080048265.01.T01 |
| Gene Sequence |
| Protein sequence |
