AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0080048266.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080048266.01.T01 MTR_4g077787 92.135 178 14 0 1 178 1 178 2.32e-123 345
MsG0080048266.01.T01 MTR_4g111640 84.615 182 26 2 1 181 1 181 1.46e-114 323
MsG0080048266.01.T01 MTR_4g112310 88.636 132 13 2 51 181 1 131 5.18e-83 241
MsG0080048266.01.T01 MTR_6g022170 59.091 110 44 1 23 132 25 133 1.26e-44 145
MsG0080048266.01.T01 MTR_2g101300 37.584 149 86 3 15 157 8 155 2.20e-33 117
MsG0080048266.01.T01 MTR_4g078410 42.636 129 68 2 29 155 22 146 2.27e-30 109
MsG0080048266.01.T01 MTR_3g105930 39.130 161 83 6 29 179 24 179 3.57e-30 108
MsG0080048266.01.T01 MTR_2g090580 37.162 148 79 4 13 150 8 151 1.99e-29 107
MsG0080048266.01.T01 MTR_1g014120 39.189 148 75 6 7 145 4 145 1.71e-28 105
MsG0080048266.01.T01 MTR_8g463180 39.286 140 71 5 12 145 5 136 4.12e-27 104
MsG0080048266.01.T01 MTR_2g079030 39.535 129 72 2 14 140 7 131 2.54e-26 104
MsG0080048266.01.T01 MTR_8g086360 34.266 143 85 3 12 152 7 142 2.36e-25 97.1
MsG0080048266.01.T01 MTR_8g095013 36.885 122 69 3 12 131 8 123 1.62e-23 94.7
MsG0080048266.01.T01 MTR_4g081100 39.423 104 59 2 31 132 31 132 5.16e-22 89.0
MsG0080048266.01.T01 MTR_2g090575 35.714 126 72 3 12 132 2 123 5.31e-22 91.3
MsG0080048266.01.T01 MTR_8g094990 36.066 122 70 3 12 131 8 123 9.77e-22 89.4
MsG0080048266.01.T01 MTR_4g124280 34.161 161 78 4 32 170 25 179 1.02e-21 88.6
MsG0080048266.01.T01 MTR_1g077790 35.652 115 71 2 29 141 26 139 1.23e-21 87.4
MsG0080048266.01.T01 MTR_7g090170 37.778 135 62 6 29 158 24 141 3.00e-21 85.1
MsG0080048266.01.T01 MTR_8g095020 40.777 103 54 3 32 132 25 122 1.34e-20 87.0
MsG0080048266.01.T01 MTR_2g083250 39.048 105 60 3 31 132 25 128 6.63e-20 84.3
MsG0080048266.01.T01 MTR_5g006040 31.944 144 90 3 14 153 8 147 1.67e-19 81.6
MsG0080048266.01.T01 MTR_1g090420 29.714 175 99 4 16 169 8 179 2.55e-18 79.3
MsG0080048266.01.T01 MTR_3g099570 31.507 146 90 4 7 150 2 139 1.80e-17 76.3
MsG0080048266.01.T01 MTR_4g114870 36.275 102 61 2 32 131 191 290 2.67e-17 78.6
MsG0080048266.01.T01 MTR_4g114870 30.714 140 88 4 13 145 7 144 4.85e-16 75.1
MsG0080048266.01.T01 MTR_1g104800 34.906 106 63 2 32 135 25 126 1.11e-16 74.3
MsG0080048266.01.T01 MTR_4g130780 32.374 139 84 5 8 140 4 138 3.56e-15 70.1
MsG0080048266.01.T01 MTR_3g099580 34.783 115 62 3 24 131 12 120 4.08e-15 70.1
MsG0080048266.01.T01 MTR_3g092170 32.540 126 70 4 12 129 2 120 4.64e-15 71.6
MsG0080048266.01.T01 MTR_4g067200 29.688 128 84 3 13 136 9 134 5.00e-15 69.7
MsG0080048266.01.T01 MTR_4g066110 29.688 128 84 3 13 136 9 134 5.00e-15 69.7
MsG0080048266.01.T01 MTR_6g023760 37.500 104 57 3 30 131 24 121 5.55e-15 69.7
MsG0080048266.01.T01 MTR_0334s0010 30.833 120 79 2 13 131 9 125 6.12e-15 70.1
MsG0080048266.01.T01 MTR_6g083240 30.519 154 95 5 14 157 8 159 1.14e-14 68.6
MsG0080048266.01.T01 MTR_2g088990 33.028 109 65 3 29 135 22 124 2.99e-14 68.6
MsG0080048266.01.T01 MTR_3g099540 29.688 128 70 4 11 131 7 121 1.10e-13 65.1
MsG0080048266.01.T01 MTR_3g099980 33.333 126 74 4 11 133 6 124 2.17e-13 66.2
MsG0080048266.01.T01 MTR_3g099680 33.333 126 74 4 11 133 6 124 2.21e-13 66.2
MsG0080048266.01.T01 MTR_6g013420 30.137 146 88 5 7 145 10 148 2.35e-13 65.1
MsG0080048266.01.T01 MTR_8g099220 28.000 125 84 3 10 131 6 127 6.36e-13 62.8
MsG0080048266.01.T01 MTR_8g007020 29.730 148 80 6 29 158 21 162 7.46e-12 61.2
MsG0080048266.01.T01 MTR_7g086100 32.031 128 78 3 7 132 2 122 1.77e-11 61.2
MsG0080048266.01.T01 MTR_2g025580 26.846 149 96 5 34 175 29 171 3.08e-11 59.3
MsG0080048266.01.T01 MTR_1g090190 28.906 128 80 3 25 147 16 137 3.26e-11 59.7
MsG0080048266.01.T01 MTR_4g130800 37.209 86 51 2 54 137 50 134 3.54e-11 60.5
MsG0080048266.01.T01 MTR_4g130800 37.209 86 51 2 54 137 49 133 4.29e-11 60.5
MsG0080048266.01.T01 MTR_8g007035 33.981 103 61 2 30 131 22 118 5.25e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080048266.01.T01 AT1G48940 47.093 172 77 3 11 173 10 176 3.12e-56 175
MsG0080048266.01.T01 AT3G18590 58.400 125 52 0 8 132 3 127 7.25e-54 169
MsG0080048266.01.T01 AT5G14345 44.262 122 66 2 25 145 9 129 7.41e-34 117
MsG0080048266.01.T01 AT1G79800 40.800 125 73 1 11 135 13 136 3.97e-32 114
MsG0080048266.01.T01 AT4G28365 35.948 153 92 2 12 162 9 157 2.02e-30 110
MsG0080048266.01.T01 AT4G31840 37.736 159 92 5 7 160 2 158 6.46e-29 105
MsG0080048266.01.T01 AT4G32490 44.860 107 53 2 28 132 27 129 4.99e-27 102
MsG0080048266.01.T01 AT4G30590 41.129 124 68 3 12 132 8 129 1.61e-26 100
MsG0080048266.01.T01 AT5G53870 35.227 176 96 6 1 167 1 167 2.46e-26 103
MsG0080048266.01.T01 AT5G53870 35.227 176 96 6 1 167 1 167 2.86e-26 103
MsG0080048266.01.T01 AT5G57920 39.552 134 70 5 18 142 6 137 1.86e-25 97.1
MsG0080048266.01.T01 AT2G25060 40.152 132 72 5 24 150 23 152 2.03e-25 97.1
MsG0080048266.01.T01 AT5G57920 41.176 119 61 4 31 142 22 138 8.34e-25 95.5
MsG0080048266.01.T01 AT5G57920 41.176 119 61 4 31 142 54 170 1.09e-24 95.9
MsG0080048266.01.T01 AT4G27520 32.298 161 97 4 5 162 4 155 2.56e-22 92.4
MsG0080048266.01.T01 AT3G20570 33.333 138 75 4 13 148 15 137 2.18e-21 87.0
MsG0080048266.01.T01 AT5G25090 39.837 123 62 6 16 132 11 127 7.88e-21 85.1
MsG0080048266.01.T01 AT2G31050 36.029 136 74 4 11 142 12 138 1.09e-20 85.1
MsG0080048266.01.T01 AT2G23990 36.296 135 75 5 1 131 1 128 5.51e-20 83.6
MsG0080048266.01.T01 AT1G64640 33.333 108 65 3 27 131 28 131 1.91e-19 81.6
MsG0080048266.01.T01 AT2G32300 30.405 148 92 4 8 150 2 143 2.62e-19 82.8
MsG0080048266.01.T01 AT2G32300 30.405 148 92 4 8 150 32 173 4.88e-19 82.4
MsG0080048266.01.T01 AT2G26720 34.559 136 76 4 11 142 12 138 8.17e-19 80.5
MsG0080048266.01.T01 AT5G26330 35.922 103 60 2 32 132 24 122 6.44e-16 72.4
MsG0080048266.01.T01 AT2G23990 32.468 154 74 6 1 131 1 147 8.15e-16 72.8
MsG0080048266.01.T01 AT3G60270 33.333 102 61 2 32 132 26 121 1.83e-15 71.2
MsG0080048266.01.T01 AT5G07475 30.667 150 92 5 14 158 13 155 2.15e-14 68.6
MsG0080048266.01.T01 AT1G45063 32.456 114 66 3 25 132 19 127 2.59e-12 63.9
MsG0080048266.01.T01 AT1G45063 33.981 103 61 3 32 131 149 247 3.01e-12 63.5
MsG0080048266.01.T01 AT1G72230 30.693 101 69 1 52 151 37 137 3.15e-12 62.4
MsG0080048266.01.T01 AT1G45063 32.143 112 68 2 24 132 21 127 4.60e-12 63.9
MsG0080048266.01.T01 AT1G45063 33.981 103 61 3 32 131 149 247 5.86e-12 63.5
MsG0080048266.01.T01 AT3G27200 33.663 101 59 3 34 132 28 122 2.36e-11 59.7
MsG0080048266.01.T01 AT3G17675 30.645 124 77 3 11 131 10 127 2.60e-11 58.9

Find 27 sgRNAs with CRISPR-Local

Find 53 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAAAATGATCATCAAGGTTT+TGG 0.254697 contig259end:-2578 MsG0080048265.01.T01:CDS
TCACAAAATAGATTCAAAAT+TGG 0.280437 contig259end:-3141 MsG0080048266.01.T01:CDS
TGTGGTGCTGGTGCTGTAAT+TGG 0.293215 contig259end:+2547 None:intergenic
CTCTTTACTTTGTCATTCCT+TGG 0.308362 contig259end:-2445 MsG0080048265.01.T01:CDS
TACTTCATCAGTGGAGTTAG+TGG 0.390509 contig259end:-2619 MsG0080048265.01.T01:CDS
GTGCATGATGTGATAGTAAT+AGG 0.401733 contig259end:+2469 None:intergenic
TCATCTTTGTCCTTTGAGTC+AGG 0.404812 contig259end:+3183 None:intergenic
TCATTTGCAGATTGTGGTGC+TGG 0.425648 contig259end:+2535 None:intergenic
TTTGAAGTTGGTGGTAGAAT+TGG 0.429149 contig259end:-3216 MsG0080048266.01.T01:CDS
CTGATGAAGTAGAACATTCC+TGG 0.429651 contig259end:+2631 None:intergenic
GCATTAGAATGTTGTGCTAT+TGG 0.440808 contig259end:+2505 None:intergenic
AGTTGGTGGTAGAATTGGTT+GGG 0.458216 contig259end:-3211 MsG0080048266.01.T01:CDS
GCATTCTCATTTGCAGATTG+TGG 0.492826 contig259end:+2529 None:intergenic
AACTGTACTGAATTTGAAGT+TGG 0.536560 contig259end:-3228 MsG0080048266.01.T01:CDS
TTGGGTTGTGCCTGACTCAA+AGG 0.571720 contig259end:-3193 MsG0080048266.01.T01:CDS
AAGTTGGTGGTAGAATTGGT+TGG 0.575738 contig259end:-3212 MsG0080048266.01.T01:CDS
AGGCCAGAAAATGATCATCA+AGG 0.582370 contig259end:-2584 MsG0080048265.01.T01:CDS
GTGTGAGGCAGCTTTGAAGA+TGG 0.582821 contig259end:+3302 None:intergenic
TGTACTGAATTTGAAGTTGG+TGG 0.583292 contig259end:-3225 MsG0080048266.01.T01:CDS
AAGATGATATGTACAACCGA+TGG 0.592618 contig259end:-3167 MsG0080048266.01.T01:CDS
GTTAGTGGTCATTGTACAAG+AGG 0.592736 contig259end:-2604 MsG0080048265.01.T01:CDS
TTCAAGCATAAATCATGCCA+AGG 0.596027 contig259end:+2428 None:intergenic
GCAAAGTAAAAGTGAGTGTG+AGG 0.606729 contig259end:+3287 None:intergenic
AGATGATATGTACAACCGAT+GGG 0.614363 contig259end:-3166 MsG0080048266.01.T01:CDS
TGAGAAAGATTCAGTGATGG+TGG 0.639612 contig259end:-2740 MsG0080048266.01.T01:CDS
GGAATGTTCTACTTCATCAG+TGG 0.644882 contig259end:-2628 MsG0080048265.01.T01:CDS
GTATGAGAAAGATTCAGTGA+TGG 0.665783 contig259end:-2743 MsG0080048266.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATCTTCATTTATAAAATAAA+TGG + contig259end:2852-2871 None:intergenic 10.0%
!!! ATTTATTTTATAAATGAAGA+TGG - contig259end:2851-2870 MsG0080048266.01.T01:intron 10.0%
!! AAAATTAAATTGGTAAGAAA+TGG + contig259end:2641-2660 None:intergenic 15.0%
!! TATATATGGCAAAATTAAAT+TGG + contig259end:2651-2670 None:intergenic 15.0%
!! ACATATTACTCCTAAAAAAA+TGG + contig259end:2775-2794 None:intergenic 20.0%
!! TCACAAAATAGATTCAAAAT+TGG - contig259end:2597-2616 MsG0080048265.01.T01:CDS 20.0%
!!! ATATATGTCACCATTTTTTT+AGG - contig259end:2762-2781 MsG0080048266.01.T01:intron 20.0%
!!! ATATCCATTACTAAAGTTTT+GGG + contig259end:2815-2834 None:intergenic 20.0%
!!! TATATCCATTACTAAAGTTT+TGG + contig259end:2816-2835 None:intergenic 20.0%
! AATTCAGAACCTTTATATGT+CGG + contig259end:2940-2959 None:intergenic 25.0%
! ATATCCCAAAACTTTAGTAA+TGG - contig259end:2808-2827 MsG0080048266.01.T01:intron 25.0%
! TGACATACATAGCATATATA+TGG + contig259end:2665-2684 None:intergenic 25.0%
ATATGTCGGATTCTACATAT+TGG + contig259end:2926-2945 None:intergenic 30.0%
ATGTAGAATCCGACATATAA+AGG - contig259end:2928-2947 MsG0080048266.01.T01:intron 30.0%
CCATTGTTGCCAAAAAATAA+TGG + contig259end:3062-3081 None:intergenic 30.0%
TTGGTAAGAAATGGTACTTA+CGG + contig259end:2632-2651 None:intergenic 30.0%
! AACTGTACTGAATTTGAAGT+TGG - contig259end:2510-2529 MsG0080048265.01.T01:CDS 30.0%
! GAAAATGATCATCAAGGTTT+TGG - contig259end:3160-3179 MsG0080048266.01.T01:CDS 30.0%
! GATGTCTATTCTTAAGTTGT+TGG - contig259end:2725-2744 MsG0080048266.01.T01:CDS 30.0%
!! AAACCTTGATGATCATTTTC+TGG + contig259end:3160-3179 None:intergenic 30.0%
!! ACAGTTGATGACCAAAAATT+GGG + contig259end:2496-2515 None:intergenic 30.0%
!! TACAGTTGATGACCAAAAAT+TGG + contig259end:2497-2516 None:intergenic 30.0%
!!! CCATTATTTTTTGGCAACAA+TGG - contig259end:3059-3078 MsG0080048266.01.T01:intron 30.0%
AAGATGATATGTACAACCGA+TGG - contig259end:2571-2590 MsG0080048265.01.T01:CDS 35.0%
AGATGATATGTACAACCGAT+GGG - contig259end:2572-2591 MsG0080048265.01.T01:CDS 35.0%
CTCTTTACTTTGTCATTCCT+TGG - contig259end:3293-3312 MsG0080048266.01.T01:CDS 35.0%
GCATTAGAATGTTGTGCTAT+TGG + contig259end:3236-3255 None:intergenic 35.0%
GTATGAGAAAGATTCAGTGA+TGG - contig259end:2995-3014 MsG0080048266.01.T01:intron 35.0%
GTGCATGATGTGATAGTAAT+AGG + contig259end:3272-3291 None:intergenic 35.0%
TAAGAAATGGTACTTACGGA+CGG + contig259end:2628-2647 None:intergenic 35.0%
! ATCTATTTTGTGAAGCCCAT+CGG + contig259end:2590-2609 None:intergenic 35.0%
! TGTACTGAATTTGAAGTTGG+TGG - contig259end:2513-2532 MsG0080048265.01.T01:CDS 35.0%
!! TTTGAAGTTGGTGGTAGAAT+TGG - contig259end:2522-2541 MsG0080048265.01.T01:CDS 35.0%
!!! TCCACTCGTCCATTATTTTT+TGG - contig259end:3050-3069 MsG0080048266.01.T01:intron 35.0%
AGGCCAGAAAATGATCATCA+AGG - contig259end:3154-3173 MsG0080048266.01.T01:CDS 40.0%
CTGATGAAGTAGAACATTCC+TGG + contig259end:3110-3129 None:intergenic 40.0%
GCAAAGTAAAAGTGAGTGTG+AGG + contig259end:2454-2473 None:intergenic 40.0%
GCATTCTCATTTGCAGATTG+TGG + contig259end:3212-3231 None:intergenic 40.0%
GGAATGTTCTACTTCATCAG+TGG - contig259end:3110-3129 MsG0080048266.01.T01:intron 40.0%
GTTAGTGGTCATTGTACAAG+AGG - contig259end:3134-3153 MsG0080048266.01.T01:CDS 40.0%
TACTTCATCAGTGGAGTTAG+TGG - contig259end:3119-3138 MsG0080048266.01.T01:intron 40.0%
TCATCTTTGTCCTTTGAGTC+AGG + contig259end:2558-2577 None:intergenic 40.0%
TGAGAAAGATTCAGTGATGG+TGG - contig259end:2998-3017 MsG0080048266.01.T01:intron 40.0%
! GTTTTCAAATTTGAGCGACC+AGG - contig259end:3089-3108 MsG0080048266.01.T01:intron 40.0%
! TTTCTCAGCCTCCCAATTTT+TGG - contig259end:2482-2501 MsG0080048265.01.T01:CDS 40.0%
!! AAGTTGGTGGTAGAATTGGT+TGG - contig259end:2526-2545 MsG0080048265.01.T01:CDS 40.0%
!! AGTTGGTGGTAGAATTGGTT+GGG - contig259end:2527-2546 MsG0080048265.01.T01:CDS 40.0%
!! GCCAAAAAATAATGGACGAG+TGG + contig259end:3054-3073 None:intergenic 40.0%
!! GTTGATGACCAAAAATTGGG+AGG + contig259end:2493-2512 None:intergenic 40.0%
TCATTTGCAGATTGTGGTGC+TGG + contig259end:3206-3225 None:intergenic 45.0%
! TTGGGTTGTGCCTGACTCAA+AGG - contig259end:2545-2564 MsG0080048265.01.T01:CDS 50.0%
!! GTGTGAGGCAGCTTTGAAGA+TGG + contig259end:2439-2458 None:intergenic 50.0%
!! TGTGGTGCTGGTGCTGTAAT+TGG + contig259end:3194-3213 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
contig259end gene 2429 3331 2429 ID=MsG0080048266.01;Name=MsG0080048266.01
contig259end mRNA 2429 3331 2429 ID=MsG0080048266.01.T01;Parent=MsG0080048266.01;Name=MsG0080048266.01.T01;_AED=0.02;_eAED=0.02;_QI=0|0|0|1|1|1|2|0|181
contig259end exon 3130 3331 3130 ID=MsG0080048266.01.T01:exon:10068;Parent=MsG0080048266.01.T01
contig259end exon 2429 2772 2429 ID=MsG0080048266.01.T01:exon:10067;Parent=MsG0080048266.01.T01
contig259end CDS 3130 3331 3130 ID=MsG0080048266.01.T01:cds;Parent=MsG0080048266.01.T01
contig259end CDS 2429 2772 2429 ID=MsG0080048266.01.T01:cds;Parent=MsG0080048266.01.T01
Gene Sequence

>MsG0080048266.01.T01

ATGGCTTCCATCTTCAAAGCTGCCTCACACTCACTTTTACTTTGCTTCATTCTTTTCTCAGCCTCCCAATTTTTGGTCATCAACTGTACTGAATTTGAAGTTGGTGGTAGAATTGGTTGGGTTGTGCCTGACTCAAAGGACAAAGATGATATGTACAACCGATGGGCTTCACAAAATAGATTCAAAATTGGTGATACCGTCCATTTCAAGTATGAGAAAGATTCAGTGATGGTGGTGAGTGAAGAAGAGTATGAACAATGCAAATCCACTCGTCCATTATTTTTTGGCAACAATGGCAACACAGTTTTCAAATTTGAGCGACCAGGAATGTTCTACTTCATCAGTGGAGTTAGTGGTCATTGTACAAGAGGCCAGAAAATGATCATCAAGGTTTTGGATGTCGAGCCAATTACAGCACCAGCACCACAATCTGCAAATGAGAATGCACCAATAGCACAACATTCTAATGCTGCTCAAATGACTCCTATTACTATCACATCATGCACACTCTTTACTTTGTCATTCCTTGGCATGATTTATGCTTGA

Protein sequence

>MsG0080048266.01.T01

MASIFKAASHSLLLCFILFSASQFLVINCTEFEVGGRIGWVVPDSKDKDDMYNRWASQNRFKIGDTVHFKYEKDSVMVVSEEEYEQCKSTRPLFFGNNGNTVFKFERPGMFYFISGVSGHCTRGQKMIIKVLDVEPITAPAPQSANENAPIAQHSNAAQMTPITITSCTLFTLSFLGMIYA*