AlfalfaGEDB | MsG0080048329.01

M. sativa cultivar ZhongmuNo.1 / MsG0080048329.01

KEGG: K01876
GO: GO:0000166 GO:0003676 GO:0004812 GO:0005524 GO:0005737 GO:0006418 GO:0016874
PFAM: PF00152 PF01336 PF02938 PF04844

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0080048329.01.T01	AES88298.2	85.407	651	47	6	1	635	12	630	0	1119

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0080048329.01.T01	F4JJT9	64.332	614	167	6	41	635	70	650	0	811

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0080048329.01.T01	G7JHA2	85.407	651	47	6	1	635	12	630	0.0	1119

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
MsG0080048329.01	MsG0480023626.01	PPI
MsG0580024400.01	MsG0080048329.01	PPI
MsG0680031431.01	MsG0080048329.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0080048329.01.T01	MTR_4g052070	85.407	651	47	6	1	635	12	630	0.0	1119
MsG0080048329.01.T01	MTR_4g120810	72.727	671	113	9	2	635	13	650	0.0	964
MsG0080048329.01.T01	MTR_8g104740	75.314	636	96	7	28	635	50	652	0.0	958
MsG0080048329.01.T01	MTR_1g113700	74.235	621	97	7	37	635	17	596	0.0	904
MsG0080048329.01.T01	MTR_4g120810	70.772	609	119	8	2	584	13	588	0.0	850
MsG0080048329.01.T01	MTR_8g104740	73.519	574	102	6	28	584	50	590	0.0	845
MsG0080048329.01.T01	MTR_8g104740	79.449	472	42	5	187	635	1	440	0.0	749
MsG0080048329.01.T01	MTR_8g104740	74.636	343	43	4	254	584	1	311	6.52e-173	502
MsG0080048329.01.T01	MTR_4g052060	93.478	184	8	3	640	821	1	182	5.10e-114	344
MsG0080048329.01.T01	MTR_4g052040	70.166	181	41	4	640	813	1	175	7.67e-68	223

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0080048329.01.T01	AT4G33760	64.332	614	167	6	41	635	70	650	0.0	811
MsG0080048329.01.T01	AT4G33760	64.534	547	142	6	108	635	1	514	0.0	725
MsG0080048329.01.T01	AT3G52525	59.091	66	26	1	730	794	61	126	3.95e-18	82.8
MsG0080048329.01.T01	AT2G36026	59.016	61	25	0	739	799	91	151	2.04e-16	78.6
MsG0080048329.01.T01	AT5G22240	37.864	103	60	2	699	800	63	162	5.54e-11	63.2

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Find 174 sgRNAs with CRISPR-Local

Find 575 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACACTGTGGTCGTTGAAA+AGG	0.465908	contig272end:-9134	MsG0080048329.01.T01:CDS
AAACCGTTGGTCTTTGTGGT+TGG	0.408501	contig272end:-17012	MsG0080048329.01.T01:CDS
AAACCTAAAACGCGAGTATG+TGG	0.521562	contig272end:-16873	MsG0080048329.01.T01:CDS
AAAGTATTGTGTGTACCTTC+TGG	0.462564	contig272end:-14579	MsG0080048329.01.T01:CDS
AAATATTCCCGGGAACCTTC+TGG	0.369193	contig272end:+15907	None:intergenic
AACCCAACCACAAAGACCAA+CGG	0.622890	contig272end:+17009	None:intergenic
AACCGTTGGTCTTTGTGGTT+GGG	0.417606	contig272end:-17011	MsG0080048329.01.T01:CDS
AACGATGTCGGTAAAACCGT+TGG	0.595077	contig272end:-17025	MsG0080048329.01.T01:CDS
AAGACATGAAATTATACGAG+AGG	0.629418	contig272end:+14880	None:intergenic
AAGCAAATGCTAATGGTAGC+TGG	0.467850	contig272end:-15708	MsG0080048329.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAACTTGATTTCAAGCTTCA+AGG	+	contig272end:9631-9650	None:intergenic	30.0%
AAGACATGAAATTATACGAG+AGG	+	contig272end:11229-11248	None:intergenic	30.0%
AAGGTAAAGATCTAAATACC+TGG	+	contig272end:10240-10259	None:intergenic	30.0%
AAGTCTGTAATGTCTATGTA+AGG	-	contig272end:9538-9557	MsG0080048329.01.T01:intron	30.0%
AATAAAAGAGGCAAAATCAC+GGG	-	contig272end:16027-16046	MsG0080048329.01.T01:intron	30.0%
AATAAGTTGTCCACTCTATT+TGG	+	contig272end:15710-15729	None:intergenic	30.0%
AATTCAAATGATGAACCTAC+TGG	+	contig272end:10071-10090	None:intergenic	30.0%
AGAAAATTGGTGAGTCAAAA+AGG	-	contig272end:15201-15220	MsG0080048329.01.T01:intron	30.0%
AGAAATGAAGAAATGTGACA+TGG	+	contig272end:14732-14751	None:intergenic	30.0%
AGATATCTAGAAGACATACA+TGG	-	contig272end:10028-10047	MsG0080048329.01.T01:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
contig272end	gene	8889	17239	8889	ID=MsG0080048329.01;Name=MsG0080048329.01
contig272end	mRNA	8889	17239	8889	ID=MsG0080048329.01.T01;Parent=MsG0080048329.01;Name=MsG0080048329.01.T01;_AED=0.24;_eAED=0.24;_QI=27\|0.64\|0.53\|0.93\|1\|1\|15\|0\|821
contig272end	exon	16686	17239	16686	ID=MsG0080048329.01.T01:exon:131;Parent=MsG0080048329.01.T01
contig272end	exon	16068	16179	16068	ID=MsG0080048329.01.T01:exon:132;Parent=MsG0080048329.01.T01
contig272end	exon	15888	15959	15888	ID=MsG0080048329.01.T01:exon:133;Parent=MsG0080048329.01.T01
contig272end	exon	15681	15772	15681	ID=MsG0080048329.01.T01:exon:134;Parent=MsG0080048329.01.T01
contig272end	exon	15380	15500	15380	ID=MsG0080048329.01.T01:exon:135;Parent=MsG0080048329.01.T01
contig272end	exon	14859	15033	14859	ID=MsG0080048329.01.T01:exon:136;Parent=MsG0080048329.01.T01
contig272end	exon	14761	14804	14761	ID=MsG0080048329.01.T01:exon:137;Parent=MsG0080048329.01.T01
contig272end	exon	14490	14670	14490	ID=MsG0080048329.01.T01:exon:138;Parent=MsG0080048329.01.T01
contig272end	exon	14218	14402	14218	ID=MsG0080048329.01.T01:exon:139;Parent=MsG0080048329.01.T01
contig272end	exon	14050	14121	14050	ID=MsG0080048329.01.T01:exon:140;Parent=MsG0080048329.01.T01
contig272end	exon	13849	13941	13849	ID=MsG0080048329.01.T01:exon:141;Parent=MsG0080048329.01.T01
contig272end	exon	13699	13746	13699	ID=MsG0080048329.01.T01:exon:142;Parent=MsG0080048329.01.T01
contig272end	exon	13485	13542	13485	ID=MsG0080048329.01.T01:exon:143;Parent=MsG0080048329.01.T01
contig272end	exon	13171	13295	13171	ID=MsG0080048329.01.T01:exon:144;Parent=MsG0080048329.01.T01
contig272end	exon	8889	9449	8889	ID=MsG0080048329.01.T01:exon:145;Parent=MsG0080048329.01.T01
contig272end	five_prime_UTR	17213	17239	17213	ID=MsG0080048329.01.T01:five_prime_utr;Parent=MsG0080048329.01.T01
contig272end	CDS	16686	17212	16686	ID=MsG0080048329.01.T01:cds;Parent=MsG0080048329.01.T01
contig272end	CDS	16068	16179	16068	ID=MsG0080048329.01.T01:cds;Parent=MsG0080048329.01.T01
contig272end	CDS	15888	15959	15888	ID=MsG0080048329.01.T01:cds;Parent=MsG0080048329.01.T01
contig272end	CDS	15681	15772	15681	ID=MsG0080048329.01.T01:cds;Parent=MsG0080048329.01.T01
contig272end	CDS	15380	15500	15380	ID=MsG0080048329.01.T01:cds;Parent=MsG0080048329.01.T01
contig272end	CDS	14859	15033	14859	ID=MsG0080048329.01.T01:cds;Parent=MsG0080048329.01.T01
contig272end	CDS	14761	14804	14761	ID=MsG0080048329.01.T01:cds;Parent=MsG0080048329.01.T01
contig272end	CDS	14490	14670	14490	ID=MsG0080048329.01.T01:cds;Parent=MsG0080048329.01.T01
contig272end	CDS	14218	14402	14218	ID=MsG0080048329.01.T01:cds;Parent=MsG0080048329.01.T01
contig272end	CDS	14050	14121	14050	ID=MsG0080048329.01.T01:cds;Parent=MsG0080048329.01.T01
contig272end	CDS	13849	13941	13849	ID=MsG0080048329.01.T01:cds;Parent=MsG0080048329.01.T01
contig272end	CDS	13699	13746	13699	ID=MsG0080048329.01.T01:cds;Parent=MsG0080048329.01.T01
contig272end	CDS	13485	13542	13485	ID=MsG0080048329.01.T01:cds;Parent=MsG0080048329.01.T01
contig272end	CDS	13171	13295	13171	ID=MsG0080048329.01.T01:cds;Parent=MsG0080048329.01.T01
contig272end	CDS	8889	9449	8889	ID=MsG0080048329.01.T01:cds;Parent=MsG0080048329.01.T01

Gene Sequence

>MsG0080048329.01.T01

ATGTCCATTCGGCGAAGCCCATCCCTTCGCAAAACCAAAATACATCTCCCTTACAAAAAGCTTAAACCCAAAACCAAAGAAGCAATCAATTTCAAAAAGCCTAAACCCCTCAACCAATCCCTCCAAAGGATTTCAAGGACTCATCATTGCGGCGAATTATCTTCCAACGATGTCGGTAAAACCGTTGGTCTTTGTGGTTGGGTTGAACTTCACAGACGCTACGGTGGAGTCGCCATTCTCATTCTTAGAGACCACACCGGCATTATTCAGGTTATTACACGTCTAGATGAATTTCCAGTCGCACATTCTGTTTTGAAAAACCTAAAACGCGAGTATGTGGCTGCCATTCAAGGTGTTGTTAGGTCTCGTCCAACTCAATCAATCAACTACGAGATGAAAACCGGCTTCATCGAGGTTGCTGCAAATGAGGTTCAAGAGTTGAATTCTAGGAATGCAAGGTTAACATTTATTCCTAAATTGAATTCTGACGATGCAGAAGGTTCTCCTAATGAGGAAATCCGTTTGAGGTATCGATGTCTTGATCTAAGGAAGCAACAAATGAATTCTAACATGTTGCTGCGGCATAATGTGGTTAAACTCATACGAAGATATCTAGAAGACATACATGGTTTTGTGGAGGTTGAAACTCCGATACTGTCAAGATCCACACCAGAAGGTTCCCGGGAATATTTAGTTCCATCAAGAATCCAGCGAGGAACATTCTATGCCTTGCCACAAAGCCCTCAGATATTTAAGCAAATGCTAATGGTAGCTGGTTTTGATAAATATTATCAAGTAGCAAGATGTTTTCGGGATGAAGATTTAAGAGCTGATGGACAACCTGAGTTCACACAACTGGATATGGAAATGGCTTTTACTCCTCTAGAGGATATGTTGTCTCTTAACGAAGAGTTGATCAGAAAGGTTTTTCTTGAAATGAAGGGTGTGGAATTACCAAACCCTTTCCCCAGGCTTACATATGCTGAAGCAATGAATAGATATGGTTCGGACCATCCAGATACCAGATTTGATCTAGAATTAAAAGATGTAATGATCCTCTCGTATAATTTCATGTCTTTCTCATTTGATATAGCTTACGGTATCCTCCGTGCGCAACTTGCGGAGACTAATCCCTCGAGCCTTGTATCTGACATTTTCTCAGGGACTTCCTTCAAGATTTTCTCTGATTCCTTGGAGTGTGGTGGAGTTATTAAAGTATTGTGTGTACCTTCTGGTGCAGCGAAGTACTCATATATTGATGTTTACAAGGAAGCAGAAAAATATGGTGCAAAGGGTTTACTTACTCTTTGGATCACGAAGAAAAGTATTGGGGGATTTTCTGCTTTAGTTTCAAGTATGGATTCTGCAACTAGAGAGGAATTGTTAAGGCGATGCTCTGCGGGACCAAGTGATCTGATTTTATTTCTTGCTGGTCATCATGCATCTGTAAATAAAACTCTAGGTCATCTTAGAGTTTATGTGGCACAAAAATTAGGTTTGATCGACCATGCCAAACATTCAATTTTGTGGATTACCGACTTTCCAATGTTTAAGTGGAATGATTCGAAGCAGAGGCTTGAGGCTTTTCATCATCCTTTCACTGCACCAAATCCTGAAGACATGAACAACCTTGCATCGGCTCGTGCATTGGCTTATGACATGGTTTTCAACGGGATTGGTGGGGGGAGTTTGAGAATTTATAAACGCGACATACAACAAAAGGCTGAAGCGAAGTATGGATATCTTCTTGAAGCTCTTGACATGGGGGCTCCGCCACATGGAGGCATAGCTTTTGGTCTGGACAGATTGGTTATGATGCTAGCTGGTGCTAATTCAATCAGGGATGTCATTGCTTTCCCAAAAACAACAACTGCACAGTGTGCCCTCACGAGATCACCTTCCGAGGAGGTTACTACAATGTCTTCCAACAAGAAAAGACTTGTGAGAGTGAGAAGAGTGTTCTCAACAAATATTGGAAGTTGTGGCTGTGCAAAAAAGAAAGCAATTGAAGTACACGAGCCTATTCAAAAGGCCAAAATTTCCATTAGCCAAATTAGCAAGGCTTCATCCAACACAACTTTAGGTGAACGAAAGGGTGCTGGTTGTAGCGTCGATCACAGCGACGATTTCTCCACCACCACTTTCTCTGAAGCTGAAACAACTCAAAACTACTCTCCACCCAAGCATAGCCCACTATCAAACACTGTGGTCGTTGAAAAGGATTCCGATGATCCATATCATGATTTCAAACACTCTATGCTTCAAATGATCTTCGAGGATGAGATCAATTCGGAGGATGATCTTCGAGTTCTTCTTCGGTGTTTTCTTCATCTCAATGATACTTATTATCACCTTGTCATCGTTAAAGTCTTTAATGATATATGTCATGAAGCTTTTCCCGACAAGGTTTGTACTACTACTTCGTCTGCTATCAAACCTAATATACATGTTTATGAAAATGAATAA

Protein sequence

>MsG0080048329.01.T01

MSIRRSPSLRKTKIHLPYKKLKPKTKEAINFKKPKPLNQSLQRISRTHHCGELSSNDVGKTVGLCGWVELHRRYGGVAILILRDHTGIIQVITRLDEFPVAHSVLKNLKREYVAAIQGVVRSRPTQSINYEMKTGFIEVAANEVQELNSRNARLTFIPKLNSDDAEGSPNEEIRLRYRCLDLRKQQMNSNMLLRHNVVKLIRRYLEDIHGFVEVETPILSRSTPEGSREYLVPSRIQRGTFYALPQSPQIFKQMLMVAGFDKYYQVARCFRDEDLRADGQPEFTQLDMEMAFTPLEDMLSLNEELIRKVFLEMKGVELPNPFPRLTYAEAMNRYGSDHPDTRFDLELKDVMILSYNFMSFSFDIAYGILRAQLAETNPSSLVSDIFSGTSFKIFSDSLECGGVIKVLCVPSGAAKYSYIDVYKEAEKYGAKGLLTLWITKKSIGGFSALVSSMDSATREELLRRCSAGPSDLILFLAGHHASVNKTLGHLRVYVAQKLGLIDHAKHSILWITDFPMFKWNDSKQRLEAFHHPFTAPNPEDMNNLASARALAYDMVFNGIGGGSLRIYKRDIQQKAEAKYGYLLEALDMGAPPHGGIAFGLDRLVMMLAGANSIRDVIAFPKTTTAQCALTRSPSEEVTTMSSNKKRLVRVRRVFSTNIGSCGCAKKKAIEVHEPIQKAKISISQISKASSNTTLGERKGAGCSVDHSDDFSTTTFSEAETTQNYSPPKHSPLSNTVVVEKDSDDPYHDFKHSMLQMIFEDEINSEDDLRVLLRCFLHLNDTYYHLVIVKVFNDICHEAFPDKVCTTTSSAIKPNIHVYENE*