Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048336.01.T01 | XP_003608030.1 | 95.146 | 103 | 5 | 0 | 2 | 104 | 3 | 105 | 7.46E-66 | 207 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048336.01.T01 | G7JMH3 | 95.146 | 103 | 5 | 0 | 2 | 104 | 3 | 105 | 3.56e-66 | 207 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048336.01 | MsG0380017115.01 | 0.813146 | 2.967412e-51 | 1.922362e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048336.01.T01 | MTR_4g086730 | 95.146 | 103 | 5 | 0 | 2 | 104 | 3 | 105 | 9.04e-70 | 207 |
| MsG0080048336.01.T01 | MTR_4g086750 | 95.146 | 103 | 4 | 1 | 3 | 104 | 4 | 106 | 1.16e-67 | 202 |
| MsG0080048336.01.T01 | MTR_4g086710 | 93.204 | 103 | 6 | 1 | 3 | 104 | 4 | 106 | 2.49e-67 | 201 |
| MsG0080048336.01.T01 | MTR_4g086740 | 91.262 | 103 | 8 | 1 | 2 | 104 | 3 | 104 | 5.15e-65 | 195 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048336.01.T01 | AT2G15780 | 45.588 | 68 | 34 | 1 | 53 | 117 | 135 | 202 | 1.52e-14 | 67.8 |
| MsG0080048336.01.T01 | AT2G15770 | 47.619 | 63 | 32 | 1 | 56 | 117 | 140 | 202 | 1.72e-12 | 62.4 |
Find 31 sgRNAs with CRISPR-Local
Find 45 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGATGATGGTTTCCAAATC+TGG | 0.268044 | contig276end:+20662 | None:intergenic |
| AATAACCGCTTCAATGTTTA+CGG | 0.279710 | contig276end:-20745 | MsG0080048336.01.T01:CDS |
| TTGTTGGTGGCTCTCAAAAT+TGG | 0.295563 | contig276end:-20602 | MsG0080048336.01.T01:CDS |
| CCACCAACAATGATCTTCTT+AGG | 0.298784 | contig276end:+20615 | None:intergenic |
| TTCAATGTTTACGGTTACTA+TGG | 0.304016 | contig276end:-20736 | MsG0080048336.01.T01:CDS |
| ACTGATTGGTGGTCCAGATT+TGG | 0.327094 | contig276end:-20675 | MsG0080048336.01.T01:CDS |
| AGAGTATCATTGAGGTAAAA+AGG | 0.344130 | contig276end:+20537 | None:intergenic |
| AACAGGAAACTCGTTGCATT+TGG | 0.348579 | contig276end:+20333 | None:intergenic |
| TTGGCTATAACTATTCTGAT+TGG | 0.361340 | contig276end:-20575 | MsG0080048336.01.T01:CDS |
| GTAATTTCAACTACACTGAT+TGG | 0.382342 | contig276end:-20689 | MsG0080048336.01.T01:CDS |
| TGGCTATAACTATTCTGATT+GGG | 0.418748 | contig276end:-20574 | MsG0080048336.01.T01:CDS |
| AACAAAGACTGGCCCTCCTT+TGG | 0.427169 | contig276end:-20711 | MsG0080048336.01.T01:CDS |
| TCTTATTTATTTGATGATGA+TGG | 0.452058 | contig276end:+20649 | None:intergenic |
| AGCGGTTATTATAACTAGTA+AGG | 0.461173 | contig276end:+20758 | None:intergenic |
| GGCTCTCAAAATTGGCACTT+TGG | 0.462653 | contig276end:-20594 | MsG0080048336.01.T01:CDS |
| AGTTATCCATAAAATCCAAC+AGG | 0.499969 | contig276end:+20316 | None:intergenic |
| CAACCTAAGAAGATCATTGT+TGG | 0.510669 | contig276end:-20618 | MsG0080048336.01.T01:CDS |
| TTACTATGGCAAACAAAGAC+TGG | 0.529422 | contig276end:-20722 | MsG0080048336.01.T01:CDS |
| AAATGCAACGAGTTTCCTGT+TGG | 0.532160 | contig276end:-20331 | MsG0080048336.01.T01:CDS |
| CCTAAGAAGATCATTGTTGG+TGG | 0.583588 | contig276end:-20615 | MsG0080048336.01.T01:CDS |
| ATTTCAACTACACTGATTGG+TGG | 0.592159 | contig276end:-20686 | MsG0080048336.01.T01:CDS |
| TCTGATTGGGCTATTAAGAG+TGG | 0.596005 | contig276end:-20561 | MsG0080048336.01.T01:CDS |
| GTAGTTGAAATTACCAAAGG+AGG | 0.596252 | contig276end:+20698 | None:intergenic |
| AGTGTAGTTGAAATTACCAA+AGG | 0.617696 | contig276end:+20695 | None:intergenic |
| GAGGACATGAACAATGTTGG+TGG | 0.628436 | contig276end:+20782 | None:intergenic |
| AGTAACCGTAAACATTGAAG+CGG | 0.634553 | contig276end:+20740 | None:intergenic |
| TAGTTGAAATTACCAAAGGA+GGG | 0.635378 | contig276end:+20699 | None:intergenic |
| GACATGAACAATGTTGGTGG+AGG | 0.645259 | contig276end:+20785 | None:intergenic |
| GCTTACCGAGAGTATCATTG+AGG | 0.656115 | contig276end:+20529 | None:intergenic |
| TTATTATAACTAGTAAGGTG+AGG | 0.684787 | contig276end:+20763 | None:intergenic |
| GGTGAGGACATGAACAATGT+TGG | 0.699900 | contig276end:+20779 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GAGTTTATAATTTATAATAA+AGG | + | contig276end:20585-20604 | None:intergenic | 10.0% |
| !! | AAGTAGAATCAAATTGTAAA+GGG | + | contig276end:20666-20685 | None:intergenic | 20.0% |
| !! | GTGTATAAATATTTGAACAA+TGG | + | contig276end:20607-20626 | None:intergenic | 20.0% |
| !! | TAAGTAGAATCAAATTGTAA+AGG | + | contig276end:20667-20686 | None:intergenic | 20.0% |
| !! | TTACAATTTGATTCTACTTA+CGG | - | contig276end:20667-20686 | MsG0080048336.01.T01:CDS | 20.0% |
| !!! | TCTTATTTATTTGATGATGA+TGG | + | contig276end:20439-20458 | None:intergenic | 20.0% |
| ! | TACAATTTGATTCTACTTAC+GGG | - | contig276end:20668-20687 | MsG0080048336.01.T01:CDS | 25.0% |
| ! | TTATTATAACTAGTAAGGTG+AGG | + | contig276end:20325-20344 | None:intergenic | 25.0% |
| AATAACCGCTTCAATGTTTA+CGG | - | contig276end:20340-20359 | MsG0080048336.01.T01:CDS | 30.0% | |
| ACAATTTGATTCTACTTACG+GGG | - | contig276end:20669-20688 | MsG0080048336.01.T01:CDS | 30.0% | |
| AGCGGTTATTATAACTAGTA+AGG | + | contig276end:20330-20349 | None:intergenic | 30.0% | |
| AGTGTAGTTGAAATTACCAA+AGG | + | contig276end:20393-20412 | None:intergenic | 30.0% | |
| AGTTATCCATAAAATCCAAC+AGG | + | contig276end:20772-20791 | None:intergenic | 30.0% | |
| GAACATTGGAGTATATGTTA+GGG | + | contig276end:20637-20656 | None:intergenic | 30.0% | |
| GTAATTTCAACTACACTGAT+TGG | - | contig276end:20396-20415 | MsG0080048336.01.T01:intron | 30.0% | |
| TAGTTGAAATTACCAAAGGA+GGG | + | contig276end:20389-20408 | None:intergenic | 30.0% | |
| TGAACATTGGAGTATATGTT+AGG | + | contig276end:20638-20657 | None:intergenic | 30.0% | |
| TGGCTATAACTATTCTGATT+GGG | - | contig276end:20511-20530 | MsG0080048336.01.T01:intron | 30.0% | |
| TTCAATGTTTACGGTTACTA+TGG | - | contig276end:20349-20368 | MsG0080048336.01.T01:CDS | 30.0% | |
| TTGGCTATAACTATTCTGAT+TGG | - | contig276end:20510-20529 | MsG0080048336.01.T01:intron | 30.0% | |
| ! | AGAGTATCATTGAGGTAAAA+AGG | + | contig276end:20551-20570 | None:intergenic | 30.0% |
| ! | TTTTACCTCAATGATACTCT+CGG | - | contig276end:20551-20570 | MsG0080048336.01.T01:CDS | 30.0% |
| AGTAACCGTAAACATTGAAG+CGG | + | contig276end:20348-20367 | None:intergenic | 35.0% | |
| CAACCTAAGAAGATCATTGT+TGG | - | contig276end:20467-20486 | MsG0080048336.01.T01:intron | 35.0% | |
| CAATTTGATTCTACTTACGG+GGG | - | contig276end:20670-20689 | MsG0080048336.01.T01:CDS | 35.0% | |
| GTAAAGGGCTAAATGAACAT+TGG | + | contig276end:20651-20670 | None:intergenic | 35.0% | |
| GTAGTTGAAATTACCAAAGG+AGG | + | contig276end:20390-20409 | None:intergenic | 35.0% | |
| TTACTATGGCAAACAAAGAC+TGG | - | contig276end:20363-20382 | MsG0080048336.01.T01:intron | 35.0% | |
| ! | ATTTCAACTACACTGATTGG+TGG | - | contig276end:20399-20418 | MsG0080048336.01.T01:intron | 35.0% |
| ! | GAGTTTCCTGTTGGATTTTA+TGG | - | contig276end:20763-20782 | MsG0080048336.01.T01:CDS | 35.0% |
| !! | ATGATGATGGTTTCCAAATC+TGG | + | contig276end:20426-20445 | None:intergenic | 35.0% |
| AAATGCAACGAGTTTCCTGT+TGG | - | contig276end:20754-20773 | MsG0080048336.01.T01:CDS | 40.0% | |
| AACAGGAAACTCGTTGCATT+TGG | + | contig276end:20755-20774 | None:intergenic | 40.0% | |
| CCACCAACAATGATCTTCTT+AGG | + | contig276end:20473-20492 | None:intergenic | 40.0% | |
| CCTAAGAAGATCATTGTTGG+TGG | - | contig276end:20470-20489 | MsG0080048336.01.T01:intron | 40.0% | |
| TCTGATTGGGCTATTAAGAG+TGG | - | contig276end:20524-20543 | MsG0080048336.01.T01:intron | 40.0% | |
| !! | TTGTTGGTGGCTCTCAAAAT+TGG | - | contig276end:20483-20502 | MsG0080048336.01.T01:intron | 40.0% |
| GAGGACATGAACAATGTTGG+TGG | + | contig276end:20306-20325 | None:intergenic | 45.0% | |
| GGCTCTCAAAATTGGCACTT+TGG | - | contig276end:20491-20510 | MsG0080048336.01.T01:intron | 45.0% | |
| GGTGAGGACATGAACAATGT+TGG | + | contig276end:20309-20328 | None:intergenic | 45.0% | |
| ! | ACTGATTGGTGGTCCAGATT+TGG | - | contig276end:20410-20429 | MsG0080048336.01.T01:intron | 45.0% |
| ! | GACATGAACAATGTTGGTGG+AGG | + | contig276end:20303-20322 | None:intergenic | 45.0% |
| !! | GCTTACCGAGAGTATCATTG+AGG | + | contig276end:20559-20578 | None:intergenic | 45.0% |
| !! | AGTTTATAATTTATAATAAA+GGG | + | contig276end:20584-20603 | None:intergenic | 5.0% |
| ! | AACAAAGACTGGCCCTCCTT+TGG | - | contig276end:20374-20393 | MsG0080048336.01.T01:intron | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| contig276end | gene | 20296 | 20811 | 20296 | ID=MsG0080048336.01;Name=MsG0080048336.01 |
| contig276end | mRNA | 20296 | 20811 | 20296 | ID=MsG0080048336.01.T01;Parent=MsG0080048336.01;Name=MsG0080048336.01.T01;_AED=0.50;_eAED=0.57;_QI=0|0|0|1|0|0.5|2|0|117 |
| contig276end | exon | 20535 | 20811 | 20535 | ID=MsG0080048336.01.T01:exon:5553;Parent=MsG0080048336.01.T01 |
| contig276end | exon | 20296 | 20372 | 20296 | ID=MsG0080048336.01.T01:exon:5554;Parent=MsG0080048336.01.T01 |
| contig276end | CDS | 20535 | 20811 | 20535 | ID=MsG0080048336.01.T01:cds;Parent=MsG0080048336.01.T01 |
| contig276end | CDS | 20296 | 20372 | 20296 | ID=MsG0080048336.01.T01:cds;Parent=MsG0080048336.01.T01 |
| Gene Sequence |
| Protein sequence |
