AlfalfaGEDB | MsG0080048336.01

M. sativa cultivar ZhongmuNo.1 / MsG0080048336.01

KEGG: -
GO: GO:0009055
PFAM: -

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0080048336.01.T01	XP_003608030.1	95.146	103	5	0	2	104	3	105	7.46E-66	207

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0080048336.01.T01	G7JMH3	95.146	103	5	0	2	104	3	105	3.56e-66	207

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048336.01	MsG0380017115.01	0.813146	2.967412e-51	1.922362e-48

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0080048336.01.T01	MTR_4g086730	95.146	103	5	0	2	104	3	105	9.04e-70	207
MsG0080048336.01.T01	MTR_4g086750	95.146	103	4	1	3	104	4	106	1.16e-67	202
MsG0080048336.01.T01	MTR_4g086710	93.204	103	6	1	3	104	4	106	2.49e-67	201
MsG0080048336.01.T01	MTR_4g086740	91.262	103	8	1	2	104	3	104	5.15e-65	195

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0080048336.01.T01	AT2G15780	45.588	68	34	1	53	117	135	202	1.52e-14	67.8
MsG0080048336.01.T01	AT2G15770	47.619	63	32	1	56	117	140	202	1.72e-12	62.4

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Find 31 sgRNAs with CRISPR-Local

Find 45 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATGCAACGAGTTTCCTGT+TGG	0.532160	contig276end:-20331	MsG0080048336.01.T01:CDS
AACAAAGACTGGCCCTCCTT+TGG	0.427169	contig276end:-20711	MsG0080048336.01.T01:CDS
AACAGGAAACTCGTTGCATT+TGG	0.348579	contig276end:+20333	None:intergenic
AATAACCGCTTCAATGTTTA+CGG	0.279710	contig276end:-20745	MsG0080048336.01.T01:CDS
ACTGATTGGTGGTCCAGATT+TGG	0.327094	contig276end:-20675	MsG0080048336.01.T01:CDS
AGAGTATCATTGAGGTAAAA+AGG	0.344130	contig276end:+20537	None:intergenic
AGCGGTTATTATAACTAGTA+AGG	0.461173	contig276end:+20758	None:intergenic
AGTAACCGTAAACATTGAAG+CGG	0.634553	contig276end:+20740	None:intergenic
AGTGTAGTTGAAATTACCAA+AGG	0.617696	contig276end:+20695	None:intergenic
AGTTATCCATAAAATCCAAC+AGG	0.499969	contig276end:+20316	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AATAACCGCTTCAATGTTTA+CGG	-	contig276end:20340-20359	MsG0080048336.01.T01:CDS	30.0%
ACAATTTGATTCTACTTACG+GGG	-	contig276end:20669-20688	MsG0080048336.01.T01:CDS	30.0%
AGCGGTTATTATAACTAGTA+AGG	+	contig276end:20330-20349	None:intergenic	30.0%
AGTGTAGTTGAAATTACCAA+AGG	+	contig276end:20393-20412	None:intergenic	30.0%
AGTTATCCATAAAATCCAAC+AGG	+	contig276end:20772-20791	None:intergenic	30.0%
GAACATTGGAGTATATGTTA+GGG	+	contig276end:20637-20656	None:intergenic	30.0%
GTAATTTCAACTACACTGAT+TGG	-	contig276end:20396-20415	MsG0080048336.01.T01:intron	30.0%
TAGTTGAAATTACCAAAGGA+GGG	+	contig276end:20389-20408	None:intergenic	30.0%
TGAACATTGGAGTATATGTT+AGG	+	contig276end:20638-20657	None:intergenic	30.0%
TGGCTATAACTATTCTGATT+GGG	-	contig276end:20511-20530	MsG0080048336.01.T01:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
contig276end	gene	20296	20811	20296	ID=MsG0080048336.01;Name=MsG0080048336.01
contig276end	mRNA	20296	20811	20296	ID=MsG0080048336.01.T01;Parent=MsG0080048336.01;Name=MsG0080048336.01.T01;_AED=0.50;_eAED=0.57;_QI=0\|0\|0\|1\|0\|0.5\|2\|0\|117
contig276end	exon	20535	20811	20535	ID=MsG0080048336.01.T01:exon:5553;Parent=MsG0080048336.01.T01
contig276end	exon	20296	20372	20296	ID=MsG0080048336.01.T01:exon:5554;Parent=MsG0080048336.01.T01
contig276end	CDS	20535	20811	20535	ID=MsG0080048336.01.T01:cds;Parent=MsG0080048336.01.T01
contig276end	CDS	20296	20372	20296	ID=MsG0080048336.01.T01:cds;Parent=MsG0080048336.01.T01

Gene Sequence	>MsG0080048336.01.T01 ATGGCCTCCACCAACATTGTTCATGTCCTCACCTTACTAGTTATAATAACCGCTTCAATGTTTACGGTTACTATGGCAAACAAAGACTGGCCCTCCTTTGGTAATTTCAACTACACTGATTGGTGGTCCAGATTTGGAAACCATCATCATCAAATAAATAAGACAGAACAACAACCTAAGAAGATCATTGTTGGTGGCTCTCAAAATTGGCACTTTGGCTATAACTATTCTGATTGGGCTATTAAGAGTGGTCCTTTTTACCTCAATGATACTCTCGTTTTCAAATATGATGCTCCAAATGCAACGAGTTTCCTGTTGGATTTTATGGATAACTTTAATTACGTTGATATATAA
Protein sequence	>MsG0080048336.01.T01 MASTNIVHVLTLLVIITASMFTVTMANKDWPSFGNFNYTDWWSRFGNHHHQINKTEQQPKKIIVGGSQNWHFGYNYSDWAIKSGPFYLNDTLVFKYDAPNATSFLLDFMDNFNYVDI*