AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380017115.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380017115.01.T01 MTR_3g101260 96.923 130 4 0 21 150 1 130 2.93e-92 263
MsG0380017115.01.T01 MTR_4g086750 46.610 118 63 0 33 150 62 179 4.90e-35 120
MsG0380017115.01.T01 MTR_4g086740 46.610 118 63 0 33 150 60 177 5.00e-35 120
MsG0380017115.01.T01 MTR_4g086730 46.610 118 63 0 33 150 61 178 6.05e-35 120
MsG0380017115.01.T01 MTR_4g086710 45.763 118 64 0 33 150 62 179 9.24e-35 119
MsG0380017115.01.T01 MTR_4g086690 42.529 87 50 0 64 150 21 107 9.43e-20 79.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380017115.01.T01 AT2G15780 42.478 113 63 1 33 145 142 252 9.77e-25 95.9
MsG0380017115.01.T01 AT2G15770 38.793 116 69 1 30 145 141 254 2.15e-23 93.2
MsG0380017115.01.T01 AT4G33930 35.897 117 58 6 43 148 229 339 7.06e-12 62.0
MsG0380017115.01.T01 AT4G34300 35.652 115 56 6 43 145 198 306 3.42e-11 60.1

Find 47 sgRNAs with CRISPR-Local

Find 57 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACCCAATGTGTCATTTATT+TGG 0.160335 3:-92214549 None:intergenic
CTTCCTTGTGATTGCTTCAA+TGG 0.264782 3:+92214414 MsG0380017115.01.T01:CDS
TCCAACCAAGATGCTTCTTT+TGG 0.296074 3:-92214464 None:intergenic
GTGATTGCTTCAATGGTTGC+AGG 0.311734 3:+92214421 MsG0380017115.01.T01:CDS
GGGAATTGGACTAAATTAAA+AGG 0.331947 3:-92215047 None:intergenic
TCATCAGAGGCAAAGTAATA+AGG 0.345121 3:-92214962 None:intergenic
TACACGACGTGTGAATTTAG+AGG 0.385924 3:+92214869 MsG0380017115.01.T01:CDS
GCTTCAATGGTTGCAGGGTC+AGG 0.401233 3:+92214427 MsG0380017115.01.T01:CDS
GACTCTGAAGGTTGGCGTGC+AGG 0.411668 3:+92214487 MsG0380017115.01.T01:CDS
AATTAAAAGGAACGTGTAGA+TGG 0.428002 3:-92215034 None:intergenic
TATTACTTTGCCTCTGATGA+AGG 0.434564 3:+92214965 MsG0380017115.01.T01:CDS
AATGCCTATGACTGTATTGC+TGG 0.454610 3:+92214989 MsG0380017115.01.T01:CDS
GAGTCCAGCAATACAGTCAT+AGG 0.464321 3:-92214993 None:intergenic
GTCGTGTAACTCCACATATT+CGG 0.471065 3:-92214854 None:intergenic
CAATGTGTCATTTATTTGGA+AGG 0.485349 3:-92214545 None:intergenic
TTTAGAGGAGCAAAGTTGCT+TGG 0.490765 3:+92214884 MsG0380017115.01.T01:CDS
TTTACTTGCTACCGAATATG+TGG 0.498287 3:+92214843 MsG0380017115.01.T01:CDS
TTCCAAATAAATGACACATT+GGG 0.508285 3:+92214547 MsG0380017115.01.T01:CDS
ATGTGTCATTTATTTGGAAG+GGG 0.525400 3:-92214543 None:intergenic
AATGTGTCATTTATTTGGAA+GGG 0.530429 3:-92214544 None:intergenic
CTTCCAAATAAATGACACAT+TGG 0.530831 3:+92214546 MsG0380017115.01.T01:CDS
CAGAACCAAAAGAAGCATCT+TGG 0.539687 3:+92214459 MsG0380017115.01.T01:CDS
ACCAAAAGAAGCATCTTGGT+TGG 0.551375 3:+92214463 MsG0380017115.01.T01:CDS
GGTTGCAGGGTCAGGAAGCA+AGG 0.552711 3:+92214435 MsG0380017115.01.T01:CDS
CAGGCACTAACTACACCCAA+TGG 0.558303 3:+92214506 MsG0380017115.01.T01:CDS
GCTGATCAAGGTGGTGGTGA+GGG 0.565763 3:+92214911 MsG0380017115.01.T01:CDS
TGATTGCTTCAATGGTTGCA+GGG 0.566600 3:+92214422 MsG0380017115.01.T01:CDS
TTCGGTAGCAAGTAAACGCT+GGG 0.568902 3:-92214836 None:intergenic
TGGTTGGAGACTCTGAAGGT+TGG 0.582794 3:+92214479 MsG0380017115.01.T01:CDS
AGGCACTAACTACACCCAAT+GGG 0.584087 3:+92214507 MsG0380017115.01.T01:CDS
ATTCGGTAGCAAGTAAACGC+TGG 0.587303 3:-92214837 None:intergenic
AACCGTGGAATTTCCTGAAG+GGG 0.598371 3:-92214810 None:intergenic
AGATGGAACAGCAATGAACT+TGG 0.599581 3:-92215017 None:intergenic
TAAAGATTGAGCTGAATCAG+TGG 0.605281 3:+92214936 MsG0380017115.01.T01:CDS
TGCTGATCAAGGTGGTGGTG+AGG 0.607379 3:+92214910 MsG0380017115.01.T01:CDS
ATAGGCATTGCCTTCATCAG+AGG 0.610448 3:-92214975 None:intergenic
ATGTCTAGAATTCTCATACA+AGG 0.613782 3:+92214385 MsG0380017115.01.T01:CDS
GGTAATGCTGATCAAGGTGG+TGG 0.619304 3:+92214905 MsG0380017115.01.T01:CDS
ACCGTGGAATTTCCTGAAGG+GGG 0.620984 3:-92214809 None:intergenic
TTGCTTGGTAATGCTGATCA+AGG 0.621103 3:+92214899 MsG0380017115.01.T01:CDS
ATCTTGGTTGGAGACTCTGA+AGG 0.629130 3:+92214475 MsG0380017115.01.T01:CDS
CAACCATTGAAGCAATCACA+AGG 0.631574 3:-92214417 None:intergenic
CAACCGTGGAATTTCCTGAA+GGG 0.654328 3:-92214811 None:intergenic
ACAACCGTGGAATTTCCTGA+AGG 0.668113 3:-92214812 None:intergenic
CTTGGTAATGCTGATCAAGG+TGG 0.670664 3:+92214902 MsG0380017115.01.T01:CDS
GTAAACGCTGGGAACAACCG+TGG 0.691075 3:-92214825 None:intergenic
CCGTGGAATTTCCTGAAGGG+GGG 0.694717 3:-92214808 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTCAAATTATTATCAA+AGG + Chr3:92214676-92214695 MsG0380017115.01.T01:intron 10.0%
!! ATAATTTGAAAAAATCAAAG+TGG - Chr3:92214670-92214689 None:intergenic 15.0%
!! TTAAATATAATTAAATGCGT+TGG - Chr3:92214647-92214666 None:intergenic 15.0%
!!! TTTGATTGATGGATTAATTT+TGG + Chr3:92214751-92214770 MsG0380017115.01.T01:intron 20.0%
! CAATATATATGCACAAACAA+TGG - Chr3:92214714-92214733 None:intergenic 25.0%
! TTCCAAATAAATGACACATT+GGG + Chr3:92214547-92214566 MsG0380017115.01.T01:CDS 25.0%
!!! AATGTGTCATTTATTTGGAA+GGG - Chr3:92214547-92214566 None:intergenic 25.0%
CTTCCAAATAAATGACACAT+TGG + Chr3:92214546-92214565 MsG0380017115.01.T01:CDS 30.0%
CTTCTTCTTCATTTGATTGA+TGG + Chr3:92214740-92214759 MsG0380017115.01.T01:intron 30.0%
! ATGTCTAGAATTCTCATACA+AGG + Chr3:92214385-92214404 MsG0380017115.01.T01:CDS 30.0%
!! ATGTGTCATTTATTTGGAAG+GGG - Chr3:92214546-92214565 None:intergenic 30.0%
!! CAATGTGTCATTTATTTGGA+AGG - Chr3:92214548-92214567 None:intergenic 30.0%
!! TACCCAATGTGTCATTTATT+TGG - Chr3:92214552-92214571 None:intergenic 30.0%
TAAAGATTGAGCTGAATCAG+TGG + Chr3:92214936-92214955 MsG0380017115.01.T01:CDS 35.0%
TATTACTTTGCCTCTGATGA+AGG + Chr3:92214965-92214984 MsG0380017115.01.T01:CDS 35.0%
TCATCAGAGGCAAAGTAATA+AGG - Chr3:92214965-92214984 None:intergenic 35.0%
! TTTACTTGCTACCGAATATG+TGG + Chr3:92214843-92214862 MsG0380017115.01.T01:CDS 35.0%
!!! GCTATTTTTTATGGCCCATT+GGG - Chr3:92214524-92214543 None:intergenic 35.0%
!!! TTGGAAGGGGCTATTTTTTA+TGG - Chr3:92214533-92214552 None:intergenic 35.0%
AATGCCTATGACTGTATTGC+TGG + Chr3:92214989-92215008 MsG0380017115.01.T01:CDS 40.0%
AGATGGAACAGCAATGAACT+TGG - Chr3:92215020-92215039 None:intergenic 40.0%
CAACCATTGAAGCAATCACA+AGG - Chr3:92214420-92214439 None:intergenic 40.0%
CTTCCTTGTGATTGCTTCAA+TGG + Chr3:92214414-92214433 MsG0380017115.01.T01:CDS 40.0%
GTCGTGTAACTCCACATATT+CGG - Chr3:92214857-92214876 None:intergenic 40.0%
TACACGACGTGTGAATTTAG+AGG + Chr3:92214869-92214888 MsG0380017115.01.T01:CDS 40.0%
TGATTGCTTCAATGGTTGCA+GGG + Chr3:92214422-92214441 MsG0380017115.01.T01:CDS 40.0%
TTTAGAGGAGCAAAGTTGCT+TGG + Chr3:92214884-92214903 MsG0380017115.01.T01:CDS 40.0%
! TCCAACCAAGATGCTTCTTT+TGG - Chr3:92214467-92214486 None:intergenic 40.0%
!! ACCAAAAGAAGCATCTTGGT+TGG + Chr3:92214463-92214482 MsG0380017115.01.T01:CDS 40.0%
!! CAGAACCAAAAGAAGCATCT+TGG + Chr3:92214459-92214478 MsG0380017115.01.T01:CDS 40.0%
!! TTGCTTGGTAATGCTGATCA+AGG + Chr3:92214899-92214918 MsG0380017115.01.T01:CDS 40.0%
!!! CAAGATGCTTCTTTTGGTTC+TGG - Chr3:92214461-92214480 None:intergenic 40.0%
!!! GGCTATTTTTTATGGCCCAT+TGG - Chr3:92214525-92214544 None:intergenic 40.0%
AACCGTGGAATTTCCTGAAG+GGG - Chr3:92214813-92214832 None:intergenic 45.0%
ACAACCGTGGAATTTCCTGA+AGG - Chr3:92214815-92214834 None:intergenic 45.0%
AGGCACTAACTACACCCAAT+GGG + Chr3:92214507-92214526 MsG0380017115.01.T01:CDS 45.0%
ATCTTGGTTGGAGACTCTGA+AGG + Chr3:92214475-92214494 MsG0380017115.01.T01:CDS 45.0%
ATTCGGTAGCAAGTAAACGC+TGG - Chr3:92214840-92214859 None:intergenic 45.0%
CAACCGTGGAATTTCCTGAA+GGG - Chr3:92214814-92214833 None:intergenic 45.0%
GAGTCCAGCAATACAGTCAT+AGG - Chr3:92214996-92215015 None:intergenic 45.0%
GTGATTGCTTCAATGGTTGC+AGG + Chr3:92214421-92214440 MsG0380017115.01.T01:CDS 45.0%
TTCGGTAGCAAGTAAACGCT+GGG - Chr3:92214839-92214858 None:intergenic 45.0%
! ATAGGCATTGCCTTCATCAG+AGG - Chr3:92214978-92214997 None:intergenic 45.0%
!! CTTGGTAATGCTGATCAAGG+TGG + Chr3:92214902-92214921 MsG0380017115.01.T01:CDS 45.0%
ACCGTGGAATTTCCTGAAGG+GGG - Chr3:92214812-92214831 None:intergenic 50.0%
CAGGCACTAACTACACCCAA+TGG + Chr3:92214506-92214525 MsG0380017115.01.T01:CDS 50.0%
GGTAATGCTGATCAAGGTGG+TGG + Chr3:92214905-92214924 MsG0380017115.01.T01:CDS 50.0%
! TGGTTGGAGACTCTGAAGGT+TGG + Chr3:92214479-92214498 MsG0380017115.01.T01:CDS 50.0%
CCCCCCTTCAGGAAATTCCA+CGG + Chr3:92214808-92214827 MsG0380017115.01.T01:CDS 55.0%
CCGTGGAATTTCCTGAAGGG+GGG - Chr3:92214811-92214830 None:intergenic 55.0%
GCTTCAATGGTTGCAGGGTC+AGG + Chr3:92214427-92214446 MsG0380017115.01.T01:CDS 55.0%
GTAAACGCTGGGAACAACCG+TGG - Chr3:92214828-92214847 None:intergenic 55.0%
GTGTTCAAGTACCCCCCTTC+AGG + Chr3:92214797-92214816 MsG0380017115.01.T01:intron 55.0%
! GCTGATCAAGGTGGTGGTGA+GGG + Chr3:92214911-92214930 MsG0380017115.01.T01:CDS 55.0%
! TGCTGATCAAGGTGGTGGTG+AGG + Chr3:92214910-92214929 MsG0380017115.01.T01:CDS 55.0%
GGTTGCAGGGTCAGGAAGCA+AGG + Chr3:92214435-92214454 MsG0380017115.01.T01:CDS 60.0%
! GACTCTGAAGGTTGGCGTGC+AGG + Chr3:92214487-92214506 MsG0380017115.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr3 gene 92214373 92215054 92214373 ID=MsG0380017115.01;Name=MsG0380017115.01
Chr3 mRNA 92214373 92215054 92214373 ID=MsG0380017115.01.T01;Parent=MsG0380017115.01;Name=MsG0380017115.01.T01;_AED=0.32;_eAED=0.33;_QI=0|0|0|1|1|1|2|0|150
Chr3 exon 92214373 92214568 92214373 ID=MsG0380017115.01.T01:exon:12542;Parent=MsG0380017115.01.T01
Chr3 exon 92214798 92215054 92214798 ID=MsG0380017115.01.T01:exon:12543;Parent=MsG0380017115.01.T01
Chr3 CDS 92214373 92214568 92214373 ID=MsG0380017115.01.T01:cds;Parent=MsG0380017115.01.T01
Chr3 CDS 92214798 92215054 92214798 ID=MsG0380017115.01.T01:cds;Parent=MsG0380017115.01.T01
Gene Sequence

>MsG0380017115.01.T01

ATGCATAAACATATGTCTAGAATTCTCATACAAGGAGTTATCTTCCTTGTGATTGCTTCAATGGTTGCAGGGTCAGGAAGCAAGGCCAGAACCAAAAGAAGCATCTTGGTTGGAGACTCTGAAGGTTGGCGTGCAGGCACTAACTACACCCAATGGGCCATAAAAAATAGCCCCTTCCAAATAAATGACACATTGGTGTTCAAGTACCCCCCTTCAGGAAATTCCACGGTTGTTCCCAGCGTTTACTTGCTACCGAATATGTGGAGTTACACGACGTGTGAATTTAGAGGAGCAAAGTTGCTTGGTAATGCTGATCAAGGTGGTGGTGAGGGCATAAAGATTGAGCTGAATCAGTGGAAGCCTTATTACTTTGCCTCTGATGAAGGCAATGCCTATGACTGTATTGCTGGACTCACCAAGTTCATTGCTGTTCCATCTACACGTTCCTTTTAA

Protein sequence

>MsG0380017115.01.T01

MHKHMSRILIQGVIFLVIASMVAGSGSKARTKRSILVGDSEGWRAGTNYTQWAIKNSPFQINDTLVFKYPPSGNSTVVPSVYLLPNMWSYTTCEFRGAKLLGNADQGGGEGIKIELNQWKPYYFASDEGNAYDCIAGLTKFIAVPSTRSF*