AlfalfaGEDB | MsG0080048351.01

M. sativa cultivar ZhongmuNo.1 / MsG0080048351.01

KEGG: K00660
GO: GO:0003824 GO:0008152
PFAM: PF00195

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0080048351.01.T01	MCH80036.1	97.368	76	2	0	1	76	70	145	6.12E-48	159

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0080048351.01.T01	P30075	98.701	77	1	0	1	77	70	146	1.00E-48	160

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0080048351.01.T01	A0A392LYL3	97.368	76	2	0	1	76	70	145	2.92e-48	159

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048345.01	MsG0080048351.01	0.841269	5.262592e-58	7.691731e-55
MsG0080048348.01	MsG0080048351.01	0.810810	9.580703e-51	5.832711e-48
MsG0080048351.01	MsG0480022736.01	0.805171	1.518374e-49	7.978178e-47

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0080048351.01.T01	MTR_1g097935	100.000	76	0	0	1	76	70	145	7.38e-50	160
MsG0080048351.01.T01	MTR_1g097910	100.000	76	0	0	1	76	70	145	7.38e-50	160
MsG0080048351.01.T01	MTR_7g016800	100.000	76	0	0	1	76	70	145	9.35e-50	160
MsG0080048351.01.T01	MTR_7g016720	100.000	76	0	0	1	76	70	145	1.03e-49	160
MsG0080048351.01.T01	MTR_7g016820	100.000	76	0	0	1	76	70	145	1.09e-49	160
MsG0080048351.01.T01	MTR_1g098140	100.000	76	0	0	1	76	70	145	1.12e-49	160
MsG0080048351.01.T01	MTR_5g007760	100.000	76	0	0	1	76	70	145	1.16e-49	160
MsG0080048351.01.T01	MTR_5g007730	98.684	76	1	0	1	76	70	145	2.94e-49	159
MsG0080048351.01.T01	MTR_7g016700	98.684	76	1	0	1	76	70	145	3.96e-49	159
MsG0080048351.01.T01	MTR_5g007713	98.684	76	1	0	1	76	70	145	4.41e-49	159
MsG0080048351.01.T01	MTR_5g007717	98.684	76	1	0	1	76	70	145	4.66e-49	159
MsG0080048351.01.T01	MTR_3g083910	96.104	77	3	0	1	77	70	146	1.72e-48	157
MsG0080048351.01.T01	MTR_7g016780	98.684	76	1	0	1	76	70	145	2.72e-48	157
MsG0080048351.01.T01	MTR_3g083920	93.421	76	5	0	1	76	70	145	9.65e-47	152
MsG0080048351.01.T01	MTR_2g058470	90.789	76	7	0	1	76	70	145	3.33e-45	149
MsG0080048351.01.T01	MTR_5g007770	88.158	76	9	0	1	76	1	76	6.66e-44	142
MsG0080048351.01.T01	MTR_3g086260	82.895	76	13	0	1	76	70	145	2.97e-42	141
MsG0080048351.01.T01	MTR_7g084300	81.579	76	14	0	1	76	70	145	1.70e-41	139
MsG0080048351.01.T01	MTR_5g007740	80.263	76	15	0	1	76	7	82	1.48e-39	132
MsG0080048351.01.T01	MTR_7g113405	98.333	60	1	0	18	77	1	60	1.44e-36	124
MsG0080048351.01.T01	MTR_3g088675	70.667	75	22	0	1	75	70	144	3.25e-34	120
MsG0080048351.01.T01	MTR_5g007723	64.474	76	13	1	1	76	107	168	6.47e-26	98.2
MsG0080048351.01.T01	MTR_3g088670	45.946	74	36	2	3	75	66	136	7.96e-12	59.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0080048351.01.T01	AT5G13930	85.526	76	11	0	1	76	75	150	3.05e-43	144

Find 25 sgRNAs with CRISPR-Local

Find 22 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTTTCAAAATCTCCTCTGTT+AGG	0.305425	contig27end:+44362	None:intergenic
TGAATACATGGCACCTTCAT+TGG	0.347646	contig27end:-44323	MsG0080048351.01.T01:CDS
GTTGTAGAGGTACCTAGATT+AGG	0.360448	contig27end:-44280	MsG0080048351.01.T01:CDS
AAGTGAGTAATCTTTGACTT+TGG	0.381814	contig27end:+44217	None:intergenic
TTGTAGAGGTACCTAGATTA+GGG	0.409559	contig27end:-44279	MsG0080048351.01.T01:CDS
TTACCAACTCACAAACTCTT+AGG	0.413497	contig27end:-44152	None:intergenic
AGTAAAAGCTATAAAAGAAT+GGG	0.422344	contig27end:-44245	MsG0080048351.01.T01:CDS
CAGTAAAAGCTATAAAAGAA+TGG	0.459818	contig27end:-44246	MsG0080048351.01.T01:CDS
GCAGCCTCCTTCCCTAATCT+AGG	0.505127	contig27end:+44268	None:intergenic
TGGCACCTTCATTGGATGCT+AGG	0.506096	contig27end:-44315	MsG0080048351.01.T01:CDS
GTGAGTTGGTAATCAGCTCC+AGG	0.508799	contig27end:+44163	None:intergenic
TCCTAGTGTTTGTGAATACA+TGG	0.528394	contig27end:-44335	MsG0080048351.01.T01:CDS
AGGCATGTCTACACCACTTG+TGG	0.569916	contig27end:+44183	None:intergenic
GCAAGACATGGTGGTTGTAG+AGG	0.570536	contig27end:-44293	MsG0080048351.01.T01:CDS
ACAAGTGGTGTAGACATGCC+TGG	0.572074	contig27end:-44181	MsG0080048351.01.T01:CDS
TCTTGCCTAGCATCCAATGA+AGG	0.579296	contig27end:+44310	None:intergenic
AGAGGTACCTAGATTAGGGA+AGG	0.585699	contig27end:-44275	MsG0080048351.01.T01:CDS
TTGATCGTTTGCACCACAAG+TGG	0.595788	contig27end:-44196	MsG0080048351.01.T01:CDS
GGTACCTAGATTAGGGAAGG+AGG	0.615819	contig27end:-44272	MsG0080048351.01.T01:CDS
AGACCTAAGAGTTTGTGAGT+TGG	0.633912	contig27end:+44149	None:intergenic
ATTGGATGCTAGGCAAGACA+TGG	0.648366	contig27end:-44305	MsG0080048351.01.T01:CDS
AGGAGATATGTACCTAACAG+AGG	0.651258	contig27end:-44374	None:intergenic
GTAAAAGCTATAAAAGAATG+GGG	0.663083	contig27end:-44244	MsG0080048351.01.T01:CDS
GCCATGTATTCACAAACACT+AGG	0.680230	contig27end:+44334	None:intergenic
GGATGCTAGGCAAGACATGG+TGG	0.737305	contig27end:-44302	MsG0080048351.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AGTAAAAGCTATAAAAGAAT+GGG	-	contig27end:44275-44294	MsG0080048351.01.T01:CDS	20.0%
!	CAGTAAAAGCTATAAAAGAA+TGG	-	contig27end:44274-44293	MsG0080048351.01.T01:CDS	25.0%
!	GTAAAAGCTATAAAAGAATG+GGG	-	contig27end:44276-44295	MsG0080048351.01.T01:CDS	25.0%
!	AAGTGAGTAATCTTTGACTT+TGG	+	contig27end:44306-44325	None:intergenic	30.0%
	CTTTCAAAATCTCCTCTGTT+AGG	+	contig27end:44161-44180	None:intergenic	35.0%
	TCCTAGTGTTTGTGAATACA+TGG	-	contig27end:44185-44204	MsG0080048351.01.T01:CDS	35.0%
!!	TTGTAGAGGTACCTAGATTA+GGG	-	contig27end:44241-44260	MsG0080048351.01.T01:CDS	35.0%
	GCCATGTATTCACAAACACT+AGG	+	contig27end:44189-44208	None:intergenic	40.0%
	TGAATACATGGCACCTTCAT+TGG	-	contig27end:44197-44216	MsG0080048351.01.T01:CDS	40.0%
!!	GTTGTAGAGGTACCTAGATT+AGG	-	contig27end:44240-44259	MsG0080048351.01.T01:CDS	40.0%
	ATTGGATGCTAGGCAAGACA+TGG	-	contig27end:44215-44234	MsG0080048351.01.T01:CDS	45.0%
	TCTTGCCTAGCATCCAATGA+AGG	+	contig27end:44213-44232	None:intergenic	45.0%
	TTGATCGTTTGCACCACAAG+TGG	-	contig27end:44324-44343	MsG0080048351.01.T01:CDS	45.0%
!!	AGAGGTACCTAGATTAGGGA+AGG	-	contig27end:44245-44264	MsG0080048351.01.T01:CDS	45.0%
	ACAAGTGGTGTAGACATGCC+TGG	-	contig27end:44339-44358	MsG0080048351.01.T01:CDS	50.0%
	AGGCATGTCTACACCACTTG+TGG	+	contig27end:44340-44359	None:intergenic	50.0%
	GCAAGACATGGTGGTTGTAG+AGG	-	contig27end:44227-44246	MsG0080048351.01.T01:CDS	50.0%
	GGTACCTAGATTAGGGAAGG+AGG	-	contig27end:44248-44267	MsG0080048351.01.T01:CDS	50.0%
	TGGCACCTTCATTGGATGCT+AGG	-	contig27end:44205-44224	MsG0080048351.01.T01:CDS	50.0%
!!	GTGAGTTGGTAATCAGCTCC+AGG	+	contig27end:44360-44379	None:intergenic	50.0%
	GCAGCCTCCTTCCCTAATCT+AGG	+	contig27end:44255-44274	None:intergenic	55.0%
	GGATGCTAGGCAAGACATGG+TGG	-	contig27end:44218-44237	MsG0080048351.01.T01:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
contig27end	gene	44153	44389	44153	ID=MsG0080048351.01;Name=MsG0080048351.01
contig27end	mRNA	44153	44389	44153	ID=MsG0080048351.01.T01;Parent=MsG0080048351.01;Name=MsG0080048351.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|78
contig27end	exon	44153	44389	44153	ID=MsG0080048351.01.T01:exon:21973;Parent=MsG0080048351.01.T01
contig27end	CDS	44153	44389	44153	ID=MsG0080048351.01.T01:cds;Parent=MsG0080048351.01.T01

Gene Sequence	>MsG0080048351.01.T01 ATGTACCTAACAGAGGAGATTTTGAAAGAGAATCCTAGTGTTTGTGAATACATGGCACCTTCATTGGATGCTAGGCAAGACATGGTGGTTGTAGAGGTACCTAGATTAGGGAAGGAGGCTGCAGTAAAAGCTATAAAAGAATGGGGTCAACCAAAGTCAAAGATTACTCACTTGATCGTTTGCACCACAAGTGGTGTAGACATGCCTGGAGCTGATTACCAACTCACAAACTCTTAG
Protein sequence	>MsG0080048351.01.T01 MYLTEEILKENPSVCEYMAPSLDARQDMVVVEVPRLGKEAAVKAIKEWGQPKSKITHLIVCTTSGVDMPGADYQLTNS*