AlfalfaGEDB | MsG0080048348.01

M. sativa cultivar ZhongmuNo.1 / MsG0080048348.01

KEGG: K00660
GO: GO:0003824 GO:0008152 GO:0009058 GO:0016746
PFAM: PF00195 PF02797

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0080048348.01.T01	XP_013469604.1	72.449	294	31	4	51	294	58	351	3.95E-135	398

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0080048348.01.T01	P51078	71.769	294	33	4	51	294	58	351	5.43E-137	395

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0080048348.01.T01	A0A396JWG4	71.333	300	29	5	52	294	124	423	6.95e-135	399

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048036.01	MsG0080048348.01	0.803934	2.750060e-49	1.399816e-46
MsG0080048301.01	MsG0080048348.01	0.805587	1.242031e-49	6.596267e-47
MsG0080048345.01	MsG0080048348.01	0.946563	3.632095e-105	6.000482e-100
MsG0080048347.01	MsG0080048348.01	0.929488	6.468590e-93	3.620256e-88
MsG0080048348.01	MsG0080048349.01	0.908787	1.166132e-81	2.201096e-77
MsG0080048348.01	MsG0080048350.01	0.922472	9.407055e-89	3.562970e-84
MsG0080048348.01	MsG0080048351.01	0.810810	9.580703e-51	5.832711e-48
MsG0080048348.01	MsG0080048352.01	0.930765	1.016616e-93	6.160844e-89
MsG0080048348.01	MsG0180000019.01	0.806668	7.357586e-50	4.016743e-47
MsG0080048348.01	MsG0180000337.01	0.812902	3.355434e-51	2.159704e-48
MsG0080048348.01	MsG0180000930.01	0.839137	1.896991e-57	2.595444e-54
MsG0080048348.01	MsG0180001050.01	0.838645	2.542771e-57	3.427567e-54
MsG0080048348.01	MsG0180001108.01	0.807551	4.783150e-50	2.671912e-47
MsG0080048348.01	MsG0180001691.01	0.806042	9.967121e-50	5.355580e-47
MsG0080048348.01	MsG0180003240.01	0.822099	2.839739e-53	2.353792e-50
MsG0080048348.01	MsG0180003248.01	0.809507	1.828605e-50	1.075591e-47
MsG0080048348.01	MsG0180003830.01	0.824631	7.269338e-54	6.475646e-51
MsG0080048348.01	MsG0180004203.01	0.823247	1.535209e-53	1.314705e-50
MsG0080048348.01	MsG0180004562.01	0.847905	8.599678e-60	1.551405e-56
MsG0080048348.01	MsG0180004942.01	0.833972	3.923451e-56	4.591846e-53
MsG0080048348.01	MsG0180005356.01	0.942158	1.178263e-101	1.446660e-96
MsG0080048348.01	MsG0180005357.01	0.950350	1.973311e-108	4.175261e-103
MsG0080048348.01	MsG0180005358.01	0.925368	2.017627e-90	8.974343e-86
MsG0080048348.01	MsG0180005648.01	0.823635	1.245549e-53	1.078507e-50
MsG0080048348.01	MsG0280006883.01	0.838056	3.606958e-57	4.775322e-54
MsG0080048348.01	MsG0280007216.01	0.803578	3.260942e-49	1.644699e-46
MsG0080048348.01	MsG0280007759.01	0.813710	2.230482e-51	1.467421e-48
MsG0080048348.01	MsG0280007911.01	0.813703	2.238434e-51	1.472356e-48
MsG0080048348.01	MsG0280008405.01	0.841251	5.321577e-58	7.773197e-55
MsG0080048348.01	MsG0280009394.01	0.801522	8.655198e-49	4.142166e-46
MsG0080048348.01	MsG0280010436.01	0.809570	1.772854e-50	1.044586e-47
MsG0080048348.01	MsG0280011053.01	0.820482	6.700380e-53	5.308055e-50
MsG0080048348.01	MsG0280011165.01	0.832725	8.022248e-56	9.044263e-53
MsG0080048348.01	MsG0280011193.01	0.809311	2.015005e-50	1.179058e-47
MsG0080048348.01	MsG0280011203.01	0.802396	5.723360e-49	2.800678e-46
MsG0080048348.01	MsG0280011238.01	0.803776	2.966838e-49	1.503884e-46
MsG0080048348.01	MsG0280011350.01	0.807420	5.099013e-50	2.838783e-47
MsG0080048348.01	MsG0380011613.01	0.873883	1.040714e-67	4.589159e-64
MsG0080048348.01	MsG0380011638.01	0.822099	2.838494e-53	2.352852e-50
MsG0080048348.01	MsG0380011974.01	0.812482	4.146510e-51	2.639608e-48
MsG0080048348.01	MsG0380012654.01	0.807960	3.916024e-50	2.211057e-47
MsG0080048348.01	MsG0380013272.01	0.809660	1.695430e-50	1.001377e-47
MsG0080048348.01	MsG0380013278.01	0.821663	3.582956e-53	2.933385e-50
MsG0080048348.01	MsG0380013300.01	0.836640	8.310214e-57	1.053540e-53
MsG0080048348.01	MsG0380013449.01	0.845744	3.355942e-59	5.648636e-56
MsG0080048348.01	MsG0380013452.01	0.851845	6.807433e-61	1.395798e-57
MsG0080048348.01	MsG0380013458.01	0.828250	9.978629e-55	9.863982e-52
MsG0080048348.01	MsG0380015010.01	0.811251	7.688165e-51	4.735541e-48
MsG0080048348.01	MsG0380016129.01	0.840277	9.581508e-58	1.357967e-54
MsG0080048348.01	MsG0380016717.01	0.814364	1.600156e-51	1.071519e-48
MsG0080048348.01	MsG0380016801.01	0.804248	2.366508e-49	1.214166e-46
MsG0080048348.01	MsG0380017151.01	0.823466	1.364102e-53	1.175445e-50
MsG0080048348.01	MsG0380017690.01	0.866266	3.232965e-65	1.083078e-61
MsG0080048348.01	MsG0380017754.01	0.800395	1.470806e-48	6.840960e-46
MsG0080048348.01	MsG0480018807.01	0.803318	3.691174e-49	1.849134e-46
MsG0080048348.01	MsG0480019295.01	0.813591	2.369045e-51	1.553549e-48
MsG0080048348.01	MsG0480019726.01	0.817560	3.095091e-52	2.261565e-49
MsG0080048348.01	MsG0480020497.01	0.809574	1.768824e-50	1.042346e-47
MsG0080048348.01	MsG0480021135.01	0.808257	3.384667e-50	1.926200e-47
MsG0080048348.01	MsG0480022125.01	0.835258	1.863667e-56	2.266887e-53
MsG0080048348.01	MsG0480022309.01	0.827251	1.734725e-54	1.665671e-51
MsG0080048348.01	MsG0480022310.01	0.812343	4.445910e-51	2.819741e-48
MsG0080048348.01	MsG0480022735.01	0.827969	1.166447e-54	1.143699e-51
MsG0080048348.01	MsG0480022736.01	0.857055	2.117262e-62	5.158525e-59
MsG0080048348.01	MsG0480023107.01	0.838285	3.148938e-57	4.197594e-54
MsG0080048348.01	MsG0480023349.01	0.813880	2.046391e-51	1.352499e-48
MsG0080048348.01	MsG0580024154.01	0.804596	2.002476e-49	1.036685e-46
MsG0080048348.01	MsG0580024218.01	0.884232	2.285317e-71	1.496183e-67
MsG0080048348.01	MsG0580024219.01	0.844329	8.088561e-59	1.301362e-55
MsG0080048348.01	MsG0580024220.01	0.827940	1.185008e-54	1.160887e-51
MsG0080048348.01	MsG0580024260.01	0.876423	1.412823e-68	6.846458e-65
MsG0080048348.01	MsG0580024344.01	0.815431	9.276095e-52	6.394290e-49
MsG0080048348.01	MsG0580024408.01	0.812047	5.160816e-51	3.246589e-48
MsG0080048348.01	MsG0580025682.01	0.821042	4.981907e-53	4.009135e-50
MsG0080048348.01	MsG0580025683.01	0.804101	2.538780e-49	1.297736e-46
MsG0080048348.01	MsG0580025870.01	0.834169	3.501390e-56	4.121239e-53
MsG0080048348.01	MsG0580027638.01	0.818937	1.510544e-52	1.146518e-49
MsG0080048348.01	MsG0580028999.01	0.852404	4.720345e-61	9.852536e-58
MsG0080048348.01	MsG0580029555.01	0.803838	2.879973e-49	1.462255e-46
MsG0080048348.01	MsG0580029583.01	0.809061	2.279027e-50	1.324671e-47
MsG0080048348.01	MsG0580029930.01	0.803613	3.205805e-49	1.618281e-46
MsG0080048348.01	MsG0580030133.01	0.853990	1.657395e-61	3.647657e-58
MsG0080048348.01	MsG0580030135.01	0.819567	1.085053e-52	8.380116e-50
MsG0080048348.01	MsG0580030145.01	0.817965	2.507957e-52	1.852937e-49
MsG0080048348.01	MsG0680030421.01	0.809730	1.637636e-50	9.690343e-48
MsG0080048348.01	MsG0680030960.01	0.805803	1.119210e-49	5.977080e-47
MsG0080048348.01	MsG0680031429.01	0.820692	5.998056e-53	4.779201e-50
MsG0080048348.01	MsG0680035841.01	0.811980	5.335065e-51	3.350321e-48
MsG0080048348.01	MsG0780035953.01	0.807136	5.858439e-50	3.237380e-47
MsG0080048348.01	MsG0780036661.01	0.856761	2.584578e-62	6.233919e-59
MsG0080048348.01	MsG0780037943.01	0.818108	2.327551e-52	1.726446e-49
MsG0080048348.01	MsG0780038755.01	0.808875	2.497695e-50	1.444532e-47
MsG0080048348.01	MsG0780038922.01	0.835393	1.722178e-56	2.103257e-53
MsG0080048348.01	MsG0780038924.01	0.824175	9.308018e-54	8.183104e-51
MsG0080048348.01	MsG0780038939.01	0.822679	2.081533e-53	1.754298e-50
MsG0080048348.01	MsG0780039190.01	0.836278	1.027826e-56	1.289244e-53
MsG0080048348.01	MsG0780039472.01	0.810645	1.039837e-50	6.302716e-48
MsG0080048348.01	MsG0780039576.01	0.872064	4.237296e-67	1.748316e-63
MsG0080048348.01	MsG0780039968.01	0.849744	2.655634e-60	5.084856e-57
MsG0080048348.01	MsG0780039978.01	0.834680	2.605125e-56	3.113712e-53
MsG0080048348.01	MsG0780040181.01	0.831337	1.767632e-55	1.912415e-52
MsG0080048348.01	MsG0780040480.01	0.821522	3.861436e-53	3.148743e-50
MsG0080048348.01	MsG0780040613.01	0.837132	6.226134e-57	8.011632e-54
MsG0080048348.01	MsG0780041198.01	0.835293	1.825769e-56	2.223232e-53
MsG0080048348.01	MsG0780041299.01	0.826599	2.483257e-54	2.339342e-51
MsG0080048348.01	MsG0780041316.01	0.842164	3.055975e-58	4.593412e-55
MsG0080048348.01	MsG0780041556.01	0.926287	5.768016e-91	2.697988e-86
MsG0080048348.01	MsG0880042740.01	0.803261	3.793041e-49	1.897398e-46
MsG0080048348.01	MsG0880042810.01	0.839345	1.675144e-57	2.306817e-54
MsG0080048348.01	MsG0880043185.01	0.812348	4.436286e-51	2.813931e-48
MsG0080048348.01	MsG0880043364.01	0.801069	1.071553e-48	5.070061e-46
MsG0080048348.01	MsG0880043824.01	0.804801	1.814780e-49	9.445465e-47
MsG0080048348.01	MsG0880043828.01	0.802381	5.764622e-49	2.819844e-46
MsG0080048348.01	MsG0880045441.01	0.857731	1.336606e-62	3.331530e-59
MsG0080048348.01	MsG0880045677.01	0.862782	3.971895e-64	1.177437e-60
MsG0080048348.01	MsG0880046429.01	0.819210	1.308914e-52	1.000884e-49
MsG0080048348.01	MsG0880046822.01	0.802416	5.669453e-49	2.775731e-46
MsG0080048348.01	MsG0880046863.01	0.837786	4.232944e-57	5.558280e-54
MsG0080048348.01	MsG0880046996.01	0.812480	4.150932e-51	2.642290e-48
MsG0080048348.01	MsG0880047081.01	0.803027	4.239799e-49	2.108205e-46
MsG0080048348.01	MsG0880047486.01	0.835017	2.142794e-56	2.587314e-53
MsG0080048348.01	MsG0880047491.01	0.824739	6.856204e-54	6.126429e-51

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0080048348.01.T01	MTR_1g097910	72.449	294	31	4	51	294	58	351	4.63e-139	398
MsG0080048348.01.T01	MTR_1g097935	72.449	294	31	4	51	294	58	351	4.78e-139	398
MsG0080048348.01.T01	MTR_1g098140	72.449	294	31	4	51	294	58	351	7.16e-139	398
MsG0080048348.01.T01	MTR_5g007730	71.769	294	33	4	51	294	58	351	5.96e-138	395
MsG0080048348.01.T01	MTR_7g016700	71.769	294	33	4	51	294	58	351	2.81e-137	394
MsG0080048348.01.T01	MTR_7g016720	71.769	294	33	4	51	294	58	351	2.94e-137	394
MsG0080048348.01.T01	MTR_7g016820	71.769	294	33	4	51	294	58	351	3.20e-137	394
MsG0080048348.01.T01	MTR_7g016800	71.769	294	33	4	51	294	58	351	3.38e-137	394
MsG0080048348.01.T01	MTR_5g007760	71.088	294	35	4	51	294	58	351	2.21e-136	392
MsG0080048348.01.T01	MTR_7g016780	70.748	294	36	4	51	294	58	351	4.17e-135	388
MsG0080048348.01.T01	MTR_5g007713	70.408	294	37	4	51	294	58	351	1.01e-134	387
MsG0080048348.01.T01	MTR_5g007717	69.697	297	40	4	51	297	58	354	1.23e-134	387
MsG0080048348.01.T01	MTR_7g113405	76.984	252	16	2	85	294	14	265	7.72e-132	377
MsG0080048348.01.T01	MTR_3g083910	68.259	293	43	4	51	293	58	350	1.95e-131	379
MsG0080048348.01.T01	MTR_3g083920	68.259	293	43	3	51	293	58	350	9.59e-131	377
MsG0080048348.01.T01	MTR_2g058470	64.333	300	50	5	51	293	51	350	1.46e-123	359
MsG0080048348.01.T01	MTR_7g084300	63.265	294	58	3	51	294	58	351	2.40e-121	353
MsG0080048348.01.T01	MTR_5g007740	62.718	287	55	4	59	293	1	287	4.85e-119	345
MsG0080048348.01.T01	MTR_3g086260	60.410	293	66	3	51	293	58	350	6.12e-112	329
MsG0080048348.01.T01	MTR_3g088675	52.901	293	88	3	51	293	58	350	4.37e-102	305
MsG0080048348.01.T01	MTR_5g007770	64.135	237	40	3	85	294	31	249	1.99e-96	286
MsG0080048348.01.T01	MTR_5g007723	47.785	316	83	7	33	294	73	360	2.17e-82	254
MsG0080048348.01.T01	MTR_3g088670	41.154	260	102	5	86	294	92	351	4.99e-60	196
MsG0080048348.01.T01	MTR_4g075560	34.169	319	145	6	42	296	43	360	9.08e-49	167
MsG0080048348.01.T01	MTR_4g078730	34.100	261	129	4	87	304	110	370	1.05e-44	156

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0080048348.01.T01	AT5G13930	64.286	294	54	3	51	293	63	356	1.29e-121	354
MsG0080048348.01.T01	AT4G00040	36.653	251	116	4	87	294	103	353	7.89e-49	167
MsG0080048348.01.T01	AT4G34850	34.921	252	120	2	87	294	31	282	1.35e-45	157
MsG0080048348.01.T01	AT4G34850	34.921	252	120	2	87	294	104	355	9.52e-45	157
MsG0080048348.01.T01	AT1G02050	38.247	251	112	5	87	294	110	360	5.09e-43	152

Find 66 sgRNAs with CRISPR-Local

Find 343 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GCAGACCATTTGCAACATTA+AGG	0.212957	contig27end:-35786	None:intergenic
CTGATTACAATTCAATCTTT+TGG	0.254027	contig27end:+40152	MsG0080048348.01.T01:CDS
TATCAACAAGGTTGCTTTGC+AGG	0.259527	contig27end:+39754	MsG0080048348.01.T01:CDS
TGCAGTGGTGATATCATTAA+TGG	0.306768	contig27end:-35737	None:intergenic
CTTGTTGGACAAGCACTATT+TGG	0.314421	contig27end:+39901	MsG0080048348.01.T01:CDS
AGAGAAGTGCTCAGTGAATA+TGG	0.317887	contig27end:+40256	MsG0080048348.01.T01:CDS
TCACCTTCTAAAAGATGTTC+CGG	0.339480	contig27end:+40070	MsG0080048348.01.T01:intron
GCTTCGTTTGGCTAAAGATT+TGG	0.342769	contig27end:+39786	MsG0080048348.01.T01:CDS
TCTACTTGATCTAGAATTGC+AGG	0.346751	contig27end:-40193	None:intergenic
CAACATTAAGGGTAGAGTAT+TGG	0.364274	contig27end:-35774	None:intergenic
AAGTGAGTAATCTTTGACTT+TGG	0.381814	contig27end:-39634	None:intergenic
AGGAGGCACGGTGCTTCGTT+TGG	0.381968	contig27end:+39774	MsG0080048348.01.T01:CDS
TACCAACTCACAAAACTCTT+AGG	0.382277	contig27end:+39700	MsG0080048348.01.T01:CDS
GGTGTAGAGGTACCTAGATT+AGG	0.406017	contig27end:+39571	MsG0080048348.01.T01:CDS
ATATTCACTGAGCACTTCTC+TGG	0.410278	contig27end:-40254	None:intergenic
CAGACCATTTGCAACATTAA+GGG	0.420881	contig27end:-35785	None:intergenic
AGTAAAAGCTATAAAAGAAT+GGG	0.422344	contig27end:+39606	MsG0080048348.01.T01:CDS
CACCTTCTAAAAGATGTTCC+GGG	0.424045	contig27end:+40071	MsG0080048348.01.T01:intron
ACTCACTTGGACAGCCTTGT+TGG	0.426712	contig27end:+39886	MsG0080048348.01.T01:CDS
ATGATTGGTGACGTCGCTTA+CGG	0.429421	contig27end:-35828	None:intergenic
GTGTAGAGGTACCTAGATTA+GGG	0.436410	contig27end:+39572	MsG0080048348.01.T01:CDS
TGGTGATATCATTAATGGTT+AGG	0.443251	contig27end:-35732	None:intergenic
GTCACTGCAGTTACATTTCG+CGG	0.450823	contig27end:+39853	MsG0080048348.01.T01:CDS
CAGTAAAAGCTATAAAAGAA+TGG	0.459818	contig27end:+39605	MsG0080048348.01.T01:CDS
CGTCGTCATCATCAAATGAT+TGG	0.462306	contig27end:-35843	None:intergenic
GTGAGTTGGTAATCAGCTCC+AGG	0.479782	contig27end:-39688	None:intergenic
CTGTCCAAGTGAGTATCACT+AGG	0.483602	contig27end:-39877	None:intergenic
TATGCGAACCTGCATTAACT+TGG	0.484888	contig27end:-35950	None:intergenic
AAAGAACATCAATAAAGCAT+TGG	0.485622	contig27end:+40102	MsG0080048348.01.T01:CDS
TCTAGAATTGCAGGTCCACC+AGG	0.500155	contig27end:-40184	None:intergenic
GTCTTCGCCCATATGTGAAA+AGG	0.504788	contig27end:+39722	MsG0080048348.01.T01:CDS
GCAGCCTCCTTCCCTAATCT+AGG	0.505127	contig27end:-39583	None:intergenic
TTCACTGAGCACTTCTCTGG+TGG	0.523654	contig27end:-40251	None:intergenic
GGACTGAAGACCACGGGAGA+AGG	0.524843	contig27end:+40341	MsG0080048348.01.T01:CDS
CGGCCCTAGTGATACTCACT+TGG	0.529910	contig27end:+39873	MsG0080048348.01.T01:CDS
GATGTTCTTTGAAACAATCC+CGG	0.530673	contig27end:-40089	None:intergenic
CATCAATAAAGCATTGGTTG+AGG	0.540608	contig27end:+40108	MsG0080048348.01.T01:CDS
TCTACCCTTAATGTTGCAAA+TGG	0.541019	contig27end:+35781	MsG0080048348.01.T01:CDS
AGGTTGCTTTGCAGGAGGCA+CGG	0.555669	contig27end:+39762	MsG0080048348.01.T01:CDS
GGACAAGCACTATTTGGAGA+TGG	0.563405	contig27end:+39907	MsG0080048348.01.T01:CDS
AGGCATGTCTACACCACTTG+TGG	0.569916	contig27end:-39668	None:intergenic
TAATGAGGCCAAGTTAATGC+AGG	0.570076	contig27end:+35942	MsG0080048348.01.T01:CDS
ACAAGTGGTGTAGACATGCC+TGG	0.572074	contig27end:+39670	MsG0080048348.01.T01:CDS
CAAATAGTGCTTGTCCAACA+AGG	0.572762	contig27end:-39900	None:intergenic
GGCAAGACATGGTGGTGTAG+AGG	0.580515	contig27end:+39558	MsG0080048348.01.T01:intron
AGAGGTACCTAGATTAGGGA+AGG	0.585699	contig27end:+39576	MsG0080048348.01.T01:CDS
GAAATGAAAGAAGTCCGCTC+AGG	0.589427	contig27end:+40316	MsG0080048348.01.T01:CDS
TGAAAGAAGTCCGCTCAGGA+TGG	0.590614	contig27end:+40320	MsG0080048348.01.T01:CDS
GATGATGACGACGATGATGA+TGG	0.592859	contig27end:+35853	MsG0080048348.01.T01:CDS
TTGATCGTTTGCACCACAAG+TGG	0.595788	contig27end:+39655	MsG0080048348.01.T01:CDS
ATTGGTGACGTCGCTTACGG+TGG	0.599236	contig27end:-35825	None:intergenic
CAGGATGGACTGAAGACCAC+GGG	0.604373	contig27end:+40335	MsG0080048348.01.T01:CDS
CCACTCAAGTCCTTCTCCCG+TGG	0.610390	contig27end:-40351	None:intergenic
GGTACCTAGATTAGGGAAGG+AGG	0.615819	contig27end:+39579	MsG0080048348.01.T01:CDS
CAACAAAGGTGCTCGTGTGT+TGG	0.620198	contig27end:+39816	MsG0080048348.01.T01:CDS
CAACAAGGTTGCTTTGCAGG+AGG	0.628329	contig27end:+39757	MsG0080048348.01.T01:CDS
ATTGGATGCTAGGCAAGACA+TGG	0.648366	contig27end:+39547	MsG0080048348.01.T01:intron
GTAAAAGCTATAAAAGAATG+GGG	0.663083	contig27end:+39607	MsG0080048348.01.T01:CDS
TGTCCAAGTGAGTATCACTA+GGG	0.674277	contig27end:-39876	None:intergenic
GGTCTTCAGTCCATCCTGAG+CGG	0.678236	contig27end:-40330	None:intergenic
TCAGGATGGACTGAAGACCA+CGG	0.679433	contig27end:+40334	MsG0080048348.01.T01:CDS
GATTTGGCTGAGAACAACAA+AGG	0.683242	contig27end:+39802	MsG0080048348.01.T01:CDS
ATGACGACGATGATGATGGT+CGG	0.710125	contig27end:+35857	MsG0080048348.01.T01:CDS
CTAGAATTGCAGGTCCACCA+GGG	0.711261	contig27end:-40183	None:intergenic
AGGTATATGATGTATCAACA+AGG	0.714291	contig27end:+39742	MsG0080048348.01.T01:CDS
GGATGCTAGGCAAGACATGG+TGG	0.737305	contig27end:+39550	MsG0080048348.01.T01:intron

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	CTAAAATAATCATTAAAAAA+GGG	+	contig27end:38785-38804	MsG0080048348.01.T01:intron	10.0%
!!	TAAAATAATCATTAAAAAAG+GGG	+	contig27end:38786-38805	MsG0080048348.01.T01:intron	10.0%
!!!	CTTTTTTAATGATTATTTTA+GGG	-	contig27end:38786-38805	None:intergenic	10.0%
!!!	TAATTTTTCATTTTGAAATA+AGG	+	contig27end:38568-38587	MsG0080048348.01.T01:intron	10.0%
!!	AAAATAAAAACTTAGAAACA+AGG	-	contig27end:36092-36111	None:intergenic	15.0%
!!	AAATAAAAACTTAGAAACAA+GGG	-	contig27end:36091-36110	None:intergenic	15.0%
!!	ATGATATTAATTAATTGATG+TGG	+	contig27end:38688-38707	MsG0080048348.01.T01:intron	15.0%
!!	ATTATCTTTACATATGTAAA+TGG	+	contig27end:39350-39369	MsG0080048348.01.T01:intron	15.0%
!!	CCTAAAATAATCATTAAAAA+AGG	+	contig27end:38784-38803	MsG0080048348.01.T01:intron	15.0%
!!!	AAATAAATCATTTTCTAGAA+AGG	-	contig27end:37702-37721	None:intergenic	15.0%
!!!	CCTTTTTTAATGATTATTTT+AGG	-	contig27end:38787-38806	None:intergenic	15.0%
!!!	TATCTATTTTCATTATTGAT+CGG	+	contig27end:38416-38435	MsG0080048348.01.T01:intron	15.0%
!!	AACAAATAACACTTAATTTC+TGG	-	contig27end:38501-38520	None:intergenic	20.0%
!!	AGTAAAAGCTATAAAAGAAT+GGG	+	contig27end:39606-39625	MsG0080048348.01.T01:CDS	20.0%
!!	CATCAAACAAAACTATATAT+TGG	+	contig27end:36231-36250	MsG0080048348.01.T01:intron	20.0%
!!	TTATTACACTATCTTTACAT+TGG	+	contig27end:39380-39399	MsG0080048348.01.T01:intron	20.0%
!!!	ATCTATTTTCATTATTGATC+GGG	+	contig27end:38417-38436	MsG0080048348.01.T01:intron	20.0%
!!!	CAATATATAGTTTTGTTTGA+TGG	-	contig27end:36233-36252	None:intergenic	20.0%
!!!	CACTTTTTTATATTCAACAA+TGG	-	contig27end:38910-38929	None:intergenic	20.0%
!!!	TAATTTCTGGAGAAAAAATT+GGG	-	contig27end:38488-38507	None:intergenic	20.0%
!!!	TTAATTTCTGGAGAAAAAAT+TGG	-	contig27end:38489-38508	None:intergenic	20.0%
!	AAAATAGGTTTCTCAATTTC+TGG	-	contig27end:39966-39985	None:intergenic	25.0%
!	AAAATATCAAAGTCCAAAGA+AGG	-	contig27end:36011-36030	None:intergenic	25.0%
!	AAAGAACATCAATAAAGCAT+TGG	+	contig27end:40102-40121	MsG0080048348.01.T01:CDS	25.0%
!	AAATGAATAAGCATGTTTAG+AGG	+	contig27end:36989-37008	MsG0080048348.01.T01:intron	25.0%
!	AAATGATAGCTAATTCACTA+TGG	-	contig27end:36154-36173	None:intergenic	25.0%
!	AAGTTTCATTTAGTGTTATG+CGG	-	contig27end:36127-36146	None:intergenic	25.0%
!	AATGAATAAGCATGTTTAGA+GGG	+	contig27end:36990-37009	MsG0080048348.01.T01:intron	25.0%
!	AATTTATTGAGAGACCATAA+AGG	-	contig27end:36340-36359	None:intergenic	25.0%
!	AATTTCTTGCAGTAAATTGA+AGG	+	contig27end:38260-38279	MsG0080048348.01.T01:intron	25.0%
!	ATTTATTGAGAGACCATAAA+GGG	-	contig27end:36339-36358	None:intergenic	25.0%
!	ATTTCTTGCAGTAAATTGAA+GGG	+	contig27end:38261-38280	MsG0080048348.01.T01:intron	25.0%
!	CAGTAAAAGCTATAAAAGAA+TGG	+	contig27end:39605-39624	MsG0080048348.01.T01:CDS	25.0%
!	GTAAAAGCTATAAAAGAATG+GGG	+	contig27end:39607-39626	MsG0080048348.01.T01:CDS	25.0%
!	TAAAAGACAAACTATTTGCA+GGG	+	contig27end:37288-37307	MsG0080048348.01.T01:intron	25.0%
!	TATATGAAGGAAAAATGGAA+GGG	-	contig27end:38972-38991	None:intergenic	25.0%
!	TATCTAAAAAAGTTAGGTAG+AGG	-	contig27end:39310-39329	None:intergenic	25.0%
!	TTAAAAGACAAACTATTTGC+AGG	+	contig27end:37287-37306	MsG0080048348.01.T01:intron	25.0%
!	TTATATGAAGGAAAAATGGA+AGG	-	contig27end:38973-38992	None:intergenic	25.0%
!	TTCTCAAGAATCAAATTGAA+CGG	+	contig27end:38525-38544	MsG0080048348.01.T01:intron	25.0%
!	TTGTTGTAAAAGAAAGAGAA+GGG	-	contig27end:39042-39061	None:intergenic	25.0%
!!	ATCATATACCTTTTCACATA+TGG	-	contig27end:39733-39752	None:intergenic	25.0%
!!	CTGATTACAATTCAATCTTT+TGG	+	contig27end:40152-40171	MsG0080048348.01.T01:CDS	25.0%
!!	TCATATACCTTTTCACATAT+GGG	-	contig27end:39732-39751	None:intergenic	25.0%
!!	TTAATCTTTTCATGATTGCA+TGG	+	contig27end:37468-37487	MsG0080048348.01.T01:intron	25.0%
!!	TTTGTTGTAAAAGAAAGAGA+AGG	-	contig27end:39043-39062	None:intergenic	25.0%
!!	TTTTTATATTCAACAATGGC+TGG	-	contig27end:38906-38925	None:intergenic	25.0%
!!!	AACATGGATTGGAAAAAAAA+TGG	+	contig27end:37751-37770	MsG0080048348.01.T01:intron	25.0%
!!!	AACTTGTGTTTTGTTAAATG+TGG	+	contig27end:36388-36407	MsG0080048348.01.T01:intron	25.0%
!!!	AATGAGATGGTTTTATATGA+AGG	-	contig27end:38985-39004	None:intergenic	25.0%
!!!	GGAAAGTATCTAAAAAAGTT+AGG	-	contig27end:39316-39335	None:intergenic	25.0%
!!!	GGTTTTATATGAAGGAAAAA+TGG	-	contig27end:38977-38996	None:intergenic	25.0%
!!!	GTCATTTTCTATTACAACTT+TGG	+	contig27end:37849-37868	MsG0080048348.01.T01:intron	25.0%
!!!	GTGATTTTAAAGTAGAAATC+AGG	-	contig27end:39224-39243	None:intergenic	25.0%
!!!	TGTTATCATTTTTGAAACTC+AGG	-	contig27end:38301-38320	None:intergenic	25.0%
!!!	TTAATTTTGCATGCATTGAT+TGG	-	contig27end:36917-36936	None:intergenic	25.0%
!!!	TTGTCTTTTAAAAAGCTGTT+TGG	-	contig27end:37278-37297	None:intergenic	25.0%
	AAAAATACTTACACATGCGT+TGG	-	contig27end:39284-39303	None:intergenic	30.0%
	AAAATGGAAACTCAGAACTT+AGG	+	contig27end:37767-37786	MsG0080048348.01.T01:intron	30.0%
	AACCAAAAAATGGCAAAGAA+TGG	+	contig27end:36503-36522	MsG0080048348.01.T01:intron	30.0%
	AACTTTCTTCTTCCGAATAA+TGG	-	contig27end:36042-36061	None:intergenic	30.0%
	AAGAACCAAAATACCTTCTT+TGG	+	contig27end:35995-36014	MsG0080048348.01.T01:intron	30.0%
	ACTTACCATCTTGATATCTT+TGG	-	contig27end:39102-39121	None:intergenic	30.0%
	AGGGGACAAATATTCTTAAT+AGG	+	contig27end:37009-37028	MsG0080048348.01.T01:intron	30.0%
	AGGTATATGATGTATCAACA+AGG	+	contig27end:39742-39761	MsG0080048348.01.T01:CDS	30.0%
	ATATATGCAACTTCCCTTTA+TGG	+	contig27end:36323-36342	MsG0080048348.01.T01:intron	30.0%
	ATCATAGATTTATCACCTGA+AGG	-	contig27end:39452-39471	None:intergenic	30.0%
	ATGAATAAGCATGTTTAGAG+GGG	+	contig27end:36991-37010	MsG0080048348.01.T01:intron	30.0%
	ATGACATTAATGTGTTGTCT+TGG	-	contig27end:36836-36855	None:intergenic	30.0%
	ATTAAAAAAGGGGATTTGAC+TGG	+	contig27end:38796-38815	MsG0080048348.01.T01:intron	30.0%
	ATTCCTTATGGTGAAAAACT+TGG	+	contig27end:38231-38250	MsG0080048348.01.T01:intron	30.0%
	ATTGGAAGGAAATAAATGCA+AGG	-	contig27end:36899-36918	None:intergenic	30.0%
	GAAAAAAGCTGAACAATAAG+TGG	-	contig27end:37496-37515	None:intergenic	30.0%
	GAACATGCTGAAAAATAATG+AGG	+	contig27end:35927-35946	MsG0080048348.01.T01:CDS	30.0%
	GAGAAAAAATTGGGACAATT+TGG	-	contig27end:38479-38498	None:intergenic	30.0%
	GATATTAGTTCAGCAAACTT+GGG	-	contig27end:39415-39434	None:intergenic	30.0%
	GGAACTCAAAAACATTACAA+AGG	+	contig27end:37129-37148	MsG0080048348.01.T01:intron	30.0%
	GGATCTAATTAGTGTTATGA+TGG	+	contig27end:38438-38457	MsG0080048348.01.T01:intron	30.0%
	GTAAAAAAGTCCAAATTGCA+AGG	-	contig27end:38370-38389	None:intergenic	30.0%
	GTGATAAATCTATGATCAAG+AGG	+	contig27end:39456-39475	MsG0080048348.01.T01:intron	30.0%
	TAAAGGGAAGTTGCATATAT+AGG	-	contig27end:36323-36342	None:intergenic	30.0%
	TCATAGATTTATCACCTGAA+GGG	-	contig27end:39451-39470	None:intergenic	30.0%
	TCATCAAGATGACTTGAAAT+AGG	-	contig27end:36278-36297	None:intergenic	30.0%
	TCTAATTAGTGTTATGATGG+TGG	+	contig27end:38441-38460	MsG0080048348.01.T01:intron	30.0%
	TCTTAACAGGTGTTAATTAG+TGG	-	contig27end:36672-36691	None:intergenic	30.0%
	TCTTATGAAAACATGACACA+TGG	-	contig27end:37805-37824	None:intergenic	30.0%
	TGAAGAAAAATCAGAGAGAT+TGG	+	contig27end:35882-35901	MsG0080048348.01.T01:CDS	30.0%
	TGATATTAGTTCAGCAAACT+TGG	-	contig27end:39416-39435	None:intergenic	30.0%
	TGGTGATATCATTAATGGTT+AGG	-	contig27end:35735-35754	None:intergenic	30.0%
	TGTCAGCATGCATTATATAT+TGG	+	contig27end:36184-36203	MsG0080048348.01.T01:intron	30.0%
!	AAAAATCAGAGAGATTGGTT+AGG	+	contig27end:35887-35906	MsG0080048348.01.T01:CDS	30.0%
!	AAGTGAGTAATCTTTGACTT+TGG	-	contig27end:39637-39656	None:intergenic	30.0%
!	AAGTTTTTCACCATAAGGAA+TGG	-	contig27end:38232-38251	None:intergenic	30.0%
!	AGGAGCTAATGTTCTTTTTA+AGG	+	contig27end:37188-37207	MsG0080048348.01.T01:intron	30.0%
!	TATCCAAGTTTTTCACCATA+AGG	-	contig27end:38237-38256	None:intergenic	30.0%
!	TTGTTTGCAACTGAAAGTAT+TGG	-	contig27end:38117-38136	None:intergenic	30.0%
!	TTTTTAGATGAATTGAAGCC+TGG	+	contig27end:37996-38015	MsG0080048348.01.T01:intron	30.0%
!!	AAAGTATTGGAACCTTAATC+CGG	-	contig27end:38104-38123	None:intergenic	30.0%
!!	TGTTTTGTTAAATGTGGCAA+TGG	+	contig27end:36394-36413	MsG0080048348.01.T01:intron	30.0%
!!	TTATCTGTTTCTGTTGGATT+TGG	-	contig27end:36553-36572	None:intergenic	30.0%
!!	TTTCATCTTTTCAGGCTTTA+AGG	-	contig27end:40230-40249	None:intergenic	30.0%
!!!	AAAGTCCAAAGAAGGTATTT+TGG	-	contig27end:36003-36022	None:intergenic	30.0%
!!!	AACCTATTTTTGAGATGGTT+TGG	+	contig27end:39976-39995	MsG0080048348.01.T01:intron	30.0%
!!!	ATCCATTCTTTGCCATTTTT+TGG	-	contig27end:36508-36527	None:intergenic	30.0%
!!!	ATGTTTTTGAGTTCCCTAAT+AGG	-	contig27end:37124-37143	None:intergenic	30.0%
!!!	CTCAGAAGTGAATTTTTTCA+AGG	+	contig27end:37414-37433	MsG0080048348.01.T01:intron	30.0%
!!!	TGAGAAACCTATTTTTGAGA+TGG	+	contig27end:39971-39990	MsG0080048348.01.T01:intron	30.0%
!!!	TGCAATTTGGACTTTTTTAC+CGG	+	contig27end:38370-38389	MsG0080048348.01.T01:intron	30.0%
!!!	TGTTTTTGAGTTCCCTAATA+GGG	-	contig27end:37123-37142	None:intergenic	30.0%
	AACTTTCAATTGAGGCTCAA+TGG	-	contig27end:37569-37588	None:intergenic	35.0%
	AAGTAAAGATGAAGAGTCCA+AGG	-	contig27end:37976-37995	None:intergenic	35.0%
	ACAAACTATTTGCAGGGTAT+TGG	+	contig27end:37294-37313	MsG0080048348.01.T01:intron	35.0%
	ACAGTACAGCAACCAAAAAA+TGG	+	contig27end:36493-36512	MsG0080048348.01.T01:intron	35.0%
	ACTTTCAATTGAGGCTCAAT+GGG	-	contig27end:37568-37587	None:intergenic	35.0%
	AGAGGATATTCAAAGTCATG+CGG	+	contig27end:38819-38838	MsG0080048348.01.T01:intron	35.0%
	AGGACAATAGTTAACGAAGA+AGG	+	contig27end:37168-37187	MsG0080048348.01.T01:intron	35.0%
	AGTGTCTCAAAAGGAAGTAA+GGG	-	contig27end:36738-36757	None:intergenic	35.0%
	ATGATCTTCTTCCTATACCT+TGG	+	contig27end:37956-37975	MsG0080048348.01.T01:intron	35.0%
	ATTCAAGAATTATGACGAGG+TGG	-	contig27end:36862-36881	None:intergenic	35.0%
	CAAACATTGTGATTGGAGTT+TGG	-	contig27end:38346-38365	None:intergenic	35.0%
	CAAACTCCAATCACAATGTT+TGG	+	contig27end:38344-38363	MsG0080048348.01.T01:intron	35.0%
	CAACATTAAGGGTAGAGTAT+TGG	-	contig27end:35777-35796	None:intergenic	35.0%
	CAGACCATTTGCAACATTAA+GGG	-	contig27end:35788-35807	None:intergenic	35.0%
	CATCTTTCATAATTCCCTTC+AGG	+	contig27end:39434-39453	MsG0080048348.01.T01:intron	35.0%
	CCTATTCAAGAATTATGACG+AGG	-	contig27end:36865-36884	None:intergenic	35.0%
	CCTCGTCATAATTCTTGAAT+AGG	+	contig27end:36862-36881	MsG0080048348.01.T01:intron	35.0%
	CTTTCAAAATCTCCTCTGTT+AGG	-	contig27end:39493-39512	None:intergenic	35.0%
	GAAAGATGTATGGAATGAGA+TGG	-	contig27end:38998-39017	None:intergenic	35.0%
	GAGATATATGTACCTAACAG+AGG	+	contig27end:39478-39497	MsG0080048348.01.T01:intron	35.0%
	GCAAAGAATGGATCAATACA+AGG	+	contig27end:36515-36534	MsG0080048348.01.T01:intron	35.0%
	GTCCAAACCATCTCAAAAAT+AGG	-	contig27end:39981-40000	None:intergenic	35.0%
	TAAAAGAAAGAGAAGGGTGA+AGG	-	contig27end:39036-39055	None:intergenic	35.0%
	TACCAACTCACAAAACTCTT+AGG	+	contig27end:39700-39719	MsG0080048348.01.T01:CDS	35.0%
	TAGAGTTGAAGACTGTTCAA+AGG	+	contig27end:35971-35990	MsG0080048348.01.T01:intron	35.0%
	TCATCTCTAAATTCTTCCCT+TGG	+	contig27end:37522-37541	MsG0080048348.01.T01:intron	35.0%
	TCCTAGTGTTTGTGAATACA+TGG	+	contig27end:39517-39536	MsG0080048348.01.T01:intron	35.0%
	TCTACCCTTAATGTTGCAAA+TGG	+	contig27end:35781-35800	MsG0080048348.01.T01:CDS	35.0%
	TGCAGACAAACTTTCAATTG+AGG	-	contig27end:37577-37596	None:intergenic	35.0%
	TGCAGTGGTGATATCATTAA+TGG	-	contig27end:35740-35759	None:intergenic	35.0%
	TGCTACCAAAGATATCAAGA+TGG	+	contig27end:39094-39113	MsG0080048348.01.T01:intron	35.0%
	TTAATTAGTGGAGCTTCTAG+TGG	-	contig27end:36660-36679	None:intergenic	35.0%
	TTCCCATTCAAACACCAAAT+GGG	+	contig27end:37720-37739	MsG0080048348.01.T01:intron	35.0%
	TTCGGAAGAAGAAAGTTACA+AGG	+	contig27end:36045-36064	MsG0080048348.01.T01:intron	35.0%
	TTCTTCTTCCGAATAATGGT+GGG	-	contig27end:36038-36057	None:intergenic	35.0%
	TTGTGTTCTTGAGAAGACAA+TGG	+	contig27end:37358-37377	MsG0080048348.01.T01:intron	35.0%
	TTTCCCATTCAAACACCAAA+TGG	+	contig27end:37719-37738	MsG0080048348.01.T01:intron	35.0%
	TTTCTTCTTCCGAATAATGG+TGG	-	contig27end:36039-36058	None:intergenic	35.0%
!	AAGGCATTTATGATTGTGTG+AGG	+	contig27end:37148-37167	MsG0080048348.01.T01:intron	35.0%
!	ACATACCTAGAGCTCTTTTT+GGG	+	contig27end:38205-38224	MsG0080048348.01.T01:intron	35.0%
!	ACTTAGAAACAAGGGTACAT+AGG	-	contig27end:36083-36102	None:intergenic	35.0%
!	CAAACTATTTGCAGGGTATT+GGG	+	contig27end:37295-37314	MsG0080048348.01.T01:intron	35.0%
!	TACATACCTAGAGCTCTTTT+TGG	+	contig27end:38204-38223	MsG0080048348.01.T01:intron	35.0%
!	TATGTAAGATGAGAAGTGTG+TGG	-	contig27end:38190-38209	None:intergenic	35.0%
!	TCACCTTCTAAAAGATGTTC+CGG	+	contig27end:40070-40089	MsG0080048348.01.T01:intron	35.0%
!	TCTACTTGATCTAGAATTGC+AGG	-	contig27end:40196-40215	None:intergenic	35.0%
!	TGCTTATTCATTTTGCGTGT+TGG	-	contig27end:36982-37001	None:intergenic	35.0%
!	TTAATGTGTTGTCTTGGTGT+TGG	-	contig27end:36830-36849	None:intergenic	35.0%
!	TTTTCTCTCCCACCATTATT+CGG	+	contig27end:36027-36046	MsG0080048348.01.T01:intron	35.0%
!!	CATCAATAAAGCATTGGTTG+AGG	+	contig27end:40108-40127	MsG0080048348.01.T01:CDS	35.0%
!!	CTACTGTTATCTGTTTCTGT+TGG	-	contig27end:36559-36578	None:intergenic	35.0%
!!	GATGTTCTTTGAAACAATCC+CGG	-	contig27end:40092-40111	None:intergenic	35.0%
!!!	ATTTGGACTTTTTTACCGGT+TGG	+	contig27end:38374-38393	MsG0080048348.01.T01:intron	35.0%
!!!	GAAGTGAATTTTTTCAAGGC+AGG	+	contig27end:37418-37437	MsG0080048348.01.T01:intron	35.0%
!!!	GATAGTTTTGAAGATTGCAG+TGG	-	contig27end:35755-35774	None:intergenic	35.0%
!!!	GGTTACTGTGATTGGTTTTT+GGG	-	contig27end:36639-36658	None:intergenic	35.0%
!!!	GTTGAGGCTTTTGAAACATT+GGG	+	contig27end:40124-40143	MsG0080048348.01.T01:CDS	35.0%
!!!	TGGTTACTGTGATTGGTTTT+TGG	-	contig27end:36640-36659	None:intergenic	35.0%
!!!	TGTTTTTTCTGGCTCTTAAC+AGG	-	contig27end:36685-36704	None:intergenic	35.0%
!!!	TTTTGCATGCATTGATTGGA+AGG	-	contig27end:36913-36932	None:intergenic	35.0%
	ACAATTGCTCCAGATAGTGA+AGG	+	contig27end:40008-40027	MsG0080048348.01.T01:intron	40.0%
	ACTCTCAATCCTCCCTATTA+GGG	+	contig27end:37108-37127	MsG0080048348.01.T01:intron	40.0%
	ACTTACCATGTAGGAGAGTT+TGG	-	contig27end:38855-38874	None:intergenic	40.0%
	ACTTCTGAGCATCAGTTAGA+GGG	-	contig27end:37403-37422	None:intergenic	40.0%
	AGATGAAGAGTCCAAGGTAT+AGG	-	contig27end:37970-37989	None:intergenic	40.0%
	AGCCAAATGAGATGGTTACA+TGG	-	contig27end:38076-38095	None:intergenic	40.0%
	AGTCCATGAACTTACCATGT+AGG	-	contig27end:38864-38883	None:intergenic	40.0%
	ATATTCACTGAGCACTTCTC+TGG	-	contig27end:40257-40276	None:intergenic	40.0%
	CAAATAGTGCTTGTCCAACA+AGG	-	contig27end:39903-39922	None:intergenic	40.0%
	CAATGGCTAAAATGGTTGCA+GGG	-	contig27end:39160-39179	None:intergenic	40.0%
	CATCTTGTCTCTATTACGAG+TGG	-	contig27end:36777-36796	None:intergenic	40.0%
	CGAAATTCGCAATGCTCAAA+GGG	+	contig27end:39127-39146	MsG0080048348.01.T01:intron	40.0%
	CGTCGTCATCATCAAATGAT+TGG	-	contig27end:35846-35865	None:intergenic	40.0%
	CTCCATGTAACCATCTCATT+TGG	+	contig27end:38071-38090	MsG0080048348.01.T01:intron	40.0%
	CTTCTAGTGGTTACTGTGAT+TGG	-	contig27end:36647-36666	None:intergenic	40.0%
	CTTGTTGGACAAGCACTATT+TGG	+	contig27end:39901-39920	MsG0080048348.01.T01:CDS	40.0%
	CTTTGTTCAACACAGTTGGT+TGG	-	contig27end:39194-39213	None:intergenic	40.0%
	GAGGATATTCAAAGTCATGC+GGG	+	contig27end:38820-38839	MsG0080048348.01.T01:intron	40.0%
	GAGTGTCTCAAAAGGAAGTA+AGG	-	contig27end:36739-36758	None:intergenic	40.0%
	GATTTGGCTGAGAACAACAA+AGG	+	contig27end:39802-39821	MsG0080048348.01.T01:CDS	40.0%
	GCAGACCATTTGCAACATTA+AGG	-	contig27end:35789-35808	None:intergenic	40.0%
	GCCATGTATTCACAAACACT+AGG	-	contig27end:39521-39540	None:intergenic	40.0%
	GCTTCGTTTGGCTAAAGATT+TGG	+	contig27end:39786-39805	MsG0080048348.01.T01:CDS	40.0%
	TAATGAGGCCAAGTTAATGC+AGG	+	contig27end:35942-35961	MsG0080048348.01.T01:CDS	40.0%
	TACTCTCAATCCTCCCTATT+AGG	+	contig27end:37107-37126	MsG0080048348.01.T01:intron	40.0%
	TATATTCAACAATGGCTGGC+TGG	-	contig27end:38902-38921	None:intergenic	40.0%
	TATCGGATACCGATACTTCT+AGG	+	contig27end:38048-38067	MsG0080048348.01.T01:intron	40.0%
	TATGCGAACCTGCATTAACT+TGG	-	contig27end:35953-35972	None:intergenic	40.0%
	TCCCATTCAAACACCAAATG+GGG	+	contig27end:37721-37740	MsG0080048348.01.T01:intron	40.0%
	TCTCCTACATGGTAAGTTCA+TGG	+	contig27end:38858-38877	MsG0080048348.01.T01:intron	40.0%
	TGAAGGAAAAATGGAAGGGT+AGG	-	contig27end:38968-38987	None:intergenic	40.0%
	TGAATACATGGCACCTTCAT+TGG	+	contig27end:39529-39548	MsG0080048348.01.T01:intron	40.0%
	TGCAGTTCCAATGGCTAAAA+TGG	-	contig27end:39168-39187	None:intergenic	40.0%
	TGTCCAAGTGAGTATCACTA+GGG	-	contig27end:39879-39898	None:intergenic	40.0%
!	AGCCTGAATGAATTTTGAGC+AGG	+	contig27end:37604-37623	MsG0080048348.01.T01:intron	40.0%
!	AGGTTAACTCGAGGCTTTTA+AGG	-	contig27end:36952-36971	None:intergenic	40.0%
!	ATCCCGGAACATCTTTTAGA+AGG	-	contig27end:40076-40095	None:intergenic	40.0%
!	ATCTTTTCGCACCAAGCAAT+GGG	+	contig27end:38141-38160	MsG0080048348.01.T01:intron	40.0%
!	ATTCATTTTGCGTGTTGGAG+TGG	-	contig27end:36977-36996	None:intergenic	40.0%
!	CACCTTCTAAAAGATGTTCC+GGG	+	contig27end:40071-40090	MsG0080048348.01.T01:intron	40.0%
!	CCTTTGAGCATTGCGAATTT+CGG	-	contig27end:39129-39148	None:intergenic	40.0%
!	GGTTGGTTGTTCATTTCGAT+GGG	-	contig27end:36610-36629	None:intergenic	40.0%
!	TACATGGAGCCTAGAAGTAT+CGG	-	contig27end:38060-38079	None:intergenic	40.0%
!	TAGCTGAACATCACGTGTTT+TGG	-	contig27end:38945-38964	None:intergenic	40.0%
!	TATCAACAAGGTTGCTTTGC+AGG	+	contig27end:39754-39773	MsG0080048348.01.T01:CDS	40.0%
!	TGTGCTTTGTTCAACACAGT+TGG	-	contig27end:39198-39217	None:intergenic	40.0%
!	TGTTGTGGTGGAAAGATGTA+TGG	-	contig27end:39008-39027	None:intergenic	40.0%
!!	AACTCGAGGCTTTTAAGGAA+AGG	-	contig27end:36947-36966	None:intergenic	40.0%
!!	ACTCGAGGCTTTTAAGGAAA+GGG	-	contig27end:36946-36965	None:intergenic	40.0%
!!	AGAGAAGTGCTCAGTGAATA+TGG	+	contig27end:40256-40275	MsG0080048348.01.T01:CDS	40.0%
!!	ATCCAAGAGAGTGTCTCAAA+AGG	-	contig27end:36747-36766	None:intergenic	40.0%
!!	CAATGTTTGGCCTTGCAATT+TGG	+	contig27end:38357-38376	MsG0080048348.01.T01:intron	40.0%
!!	GACCTAAGAGTTTTGTGAGT+TGG	-	contig27end:39705-39724	None:intergenic	40.0%
!!	GGCTTTTAAGGAAAGGGTTT+TGG	-	contig27end:36940-36959	None:intergenic	40.0%
!!	GGTTTCTCAATTTCTGGTAC+TGG	-	contig27end:39960-39979	None:intergenic	40.0%
!!	GTGTAGAGGTACCTAGATTA+GGG	+	contig27end:39572-39591	MsG0080048348.01.T01:CDS	40.0%
!!	TCTGTTTCTGTTGGATTTGG+TGG	-	contig27end:36550-36569	None:intergenic	40.0%
!!!	CTTTTTGGGACCATTCCTTA+TGG	+	contig27end:38219-38238	MsG0080048348.01.T01:intron	40.0%
!!!	GGTTGAGGCTTTTGAAACAT+TGG	+	contig27end:40123-40142	MsG0080048348.01.T01:CDS	40.0%
!!!	TGATTGGTTTTTGGGAGTTG+CGG	-	contig27end:36631-36650	None:intergenic	40.0%
!!!	TTCCTTTTGAGACACTCTCT+TGG	+	contig27end:36742-36761	MsG0080048348.01.T01:intron	40.0%
!!!	TTTTGAGTTCCCTAATAGGG+AGG	-	contig27end:37120-37139	None:intergenic	40.0%
	AAAAGGGGATTTGACTGGAG+AGG	+	contig27end:38801-38820	MsG0080048348.01.T01:intron	45.0%
	AGAGTGGTGTGGATAGGAAT+AGG	+	contig27end:37321-37340	MsG0080048348.01.T01:intron	45.0%
	ATGGTCCCAAAAAGAGCTCT+AGG	-	contig27end:38213-38232	None:intergenic	45.0%
	ATGTTAGTCCAGCTTCACGA+AGG	-	contig27end:40049-40068	None:intergenic	45.0%
	ATTCCTATCCACACCACTCT+TGG	-	contig27end:37321-37340	None:intergenic	45.0%
	ATTGGATGCTAGGCAAGACA+TGG	+	contig27end:39547-39566	MsG0080048348.01.T01:intron	45.0%
	CAAATGGGGGCAATAGAACA+TGG	+	contig27end:37735-37754	MsG0080048348.01.T01:intron	45.0%
	CACTTCTGAGCATCAGTTAG+AGG	-	contig27end:37404-37423	None:intergenic	45.0%
	CATGTTCTATTGCCCCCATT+TGG	-	contig27end:37737-37756	None:intergenic	45.0%
	CCAATGGCTAAAATGGTTGC+AGG	-	contig27end:39161-39180	None:intergenic	45.0%
	CCCATTCAAACACCAAATGG+GGG	+	contig27end:37722-37741	MsG0080048348.01.T01:intron	45.0%
	CCGAAATTCGCAATGCTCAA+AGG	+	contig27end:39126-39145	MsG0080048348.01.T01:intron	45.0%
	CTGTCCAAGTGAGTATCACT+AGG	-	contig27end:39880-39899	None:intergenic	45.0%
	GAAATGAAAGAAGTCCGCTC+AGG	+	contig27end:40316-40335	MsG0080048348.01.T01:CDS	45.0%
	GATAGTGAAGGAGCCATTGA+TGG	+	contig27end:40020-40039	MsG0080048348.01.T01:intron	45.0%
	GCAAGGCCAAACATTGTGAT+TGG	-	contig27end:38353-38372	None:intergenic	45.0%
	GCATCGAGATGTACCTGTAT+CGG	+	contig27end:38031-38050	MsG0080048348.01.T01:intron	45.0%
	GGACAAGCACTATTTGGAGA+TGG	+	contig27end:39907-39926	MsG0080048348.01.T01:CDS	45.0%
	GGCCTGCTCAAAATTCATTC+AGG	-	contig27end:37609-37628	None:intergenic	45.0%
	GGTTGGATTTGCAGTTCCAA+TGG	-	contig27end:39177-39196	None:intergenic	45.0%
	GTCACTGCAGTTACATTTCG+CGG	+	contig27end:39853-39872	MsG0080048348.01.T01:CDS	45.0%
	GTCCACAATTACTGTCCAAC+CGG	-	contig27end:38392-38411	None:intergenic	45.0%
	GTCTCAAAAGGAAGTAAGGG+CGG	-	contig27end:36735-36754	None:intergenic	45.0%
	GTCTCTATTACGAGTGGTGT+TGG	-	contig27end:36771-36790	None:intergenic	45.0%
	TACAGGTACATCTCGATGCT+TGG	-	contig27end:38030-38049	None:intergenic	45.0%
	TACCATGTAGGAGAGTTTGG+TGG	-	contig27end:38852-38871	None:intergenic	45.0%
	TCAATGGCTCCTTCACTATC+TGG	-	contig27end:40020-40039	None:intergenic	45.0%
	TCTTGCCTAGCATCCAATGA+AGG	-	contig27end:39545-39564	None:intergenic	45.0%
	TTCACGAAGGTGACCATCAA+TGG	-	contig27end:40036-40055	None:intergenic	45.0%
	TTGATCGTTTGCACCACAAG+TGG	+	contig27end:39655-39674	MsG0080048348.01.T01:CDS	45.0%
!	AAGTAAGGGCGGTGTTTTGT+TGG	-	contig27end:36724-36743	None:intergenic	45.0%
!	ATCTTTTGGATTGCACACCC+TGG	+	contig27end:40166-40185	MsG0080048348.01.T01:CDS	45.0%
!	CCTAGCACAAGAAGTGCAAA+AGG	+	contig27end:36463-36482	MsG0080048348.01.T01:intron	45.0%
!	CCTTTTGCACTTCTTGTGCT+AGG	-	contig27end:36466-36485	None:intergenic	45.0%
!	CGGTTGGTTGTTCATTTCGA+TGG	-	contig27end:36611-36630	None:intergenic	45.0%
!	CTGGTGGCTTTCATCTTTTC+AGG	-	contig27end:40238-40257	None:intergenic	45.0%
!	GAATGAATTTTGAGCAGGCC+TGG	+	contig27end:37609-37628	MsG0080048348.01.T01:intron	45.0%
!	GATCTTTTCGCACCAAGCAA+TGG	+	contig27end:38140-38159	MsG0080048348.01.T01:intron	45.0%
!	GATGATGACGACGATGATGA+TGG	+	contig27end:35853-35872	MsG0080048348.01.T01:CDS	45.0%
!	TACCGGTTGGACAGTAATTG+TGG	+	contig27end:38387-38406	MsG0080048348.01.T01:intron	45.0%
!!	AGAGGTACCTAGATTAGGGA+AGG	+	contig27end:39576-39595	MsG0080048348.01.T01:CDS	45.0%
!!	ATGACGACGATGATGATGGT+CGG	+	contig27end:35857-35876	MsG0080048348.01.T01:CDS	45.0%
!!	ATGATTGGTGACGTCGCTTA+CGG	-	contig27end:35831-35850	None:intergenic	45.0%
!!	CATCTCATTTGGCTTTGAGC+CGG	+	contig27end:38082-38101	MsG0080048348.01.T01:intron	45.0%
!!	CCCCCATTTGGTGTTTGAAT+GGG	-	contig27end:37725-37744	None:intergenic	45.0%
!!	CCTGCAACCATTTTAGCCAT+TGG	+	contig27end:39158-39177	MsG0080048348.01.T01:intron	45.0%
!!	GAAGTATCGGTATCCGATAC+AGG	-	contig27end:38047-38066	None:intergenic	45.0%
!!	GGTGTAGAGGTACCTAGATT+AGG	+	contig27end:39571-39590	MsG0080048348.01.T01:CDS	45.0%
!!	GTTATGATGGTGGTTCACGT+AGG	+	contig27end:38451-38470	MsG0080048348.01.T01:intron	45.0%
!!	TTTGGCTTTGAGCCGGATTA+AGG	+	contig27end:38089-38108	MsG0080048348.01.T01:intron	45.0%
!!!	GCAGGTGGGATTGTTTTTTC+TGG	-	contig27end:36696-36715	None:intergenic	45.0%
!!	AAAAAAATAAATAATTTACA+TGG	-	contig27end:37673-37692	None:intergenic	5.0%
!!	AAAAAATAAATAATTTACAT+GGG	-	contig27end:37672-37691	None:intergenic	5.0%
!!	AGATAATAAATATATTTAAA+TGG	-	contig27end:39337-39356	None:intergenic	5.0%
	ACAAGTGGTGTAGACATGCC+TGG	+	contig27end:39670-39689	MsG0080048348.01.T01:CDS	50.0%
	AGCCTTTCAGAGAAAGCTCC+AGG	-	contig27end:37630-37649	None:intergenic	50.0%
	AGGCATGTCTACACCACTTG+TGG	-	contig27end:39671-39690	None:intergenic	50.0%
	AGTGTGTGGTTAGAGCCTAG+AGG	-	contig27end:38176-38195	None:intergenic	50.0%
	CGGCTCAAAGCCAAATGAGA+TGG	-	contig27end:38084-38103	None:intergenic	50.0%
	CTAGAATTGCAGGTCCACCA+GGG	-	contig27end:40186-40205	None:intergenic	50.0%
	GGGCTAACTGAATCCTTGCA+GGG	-	contig27end:37548-37567	None:intergenic	50.0%
	GGGGGCAATAGAACATGGAT+TGG	+	contig27end:37740-37759	MsG0080048348.01.T01:intron	50.0%
	GGTACCTAGATTAGGGAAGG+AGG	+	contig27end:39579-39598	MsG0080048348.01.T01:CDS	50.0%
	GGTTAGAGCCTAGAGGTTGT+CGG	-	contig27end:38169-38188	None:intergenic	50.0%
	GTGGTGTGGATAGGAATAGG+TGG	+	contig27end:37324-37343	MsG0080048348.01.T01:intron	50.0%
	TGAAAGAAGTCCGCTCAGGA+TGG	+	contig27end:40320-40339	MsG0080048348.01.T01:CDS	50.0%
	TGCCACCAAACTCTCCTACA+TGG	+	contig27end:38847-38866	MsG0080048348.01.T01:intron	50.0%
	TGGCACCTTCATTGGATGCT+AGG	+	contig27end:39537-39556	MsG0080048348.01.T01:intron	50.0%
	TGGGCTAACTGAATCCTTGC+AGG	-	contig27end:37549-37568	None:intergenic	50.0%
	TTCACTGAGCACTTCTCTGG+TGG	-	contig27end:40254-40273	None:intergenic	50.0%
!	ACTCACTTGGACAGCCTTGT+TGG	+	contig27end:39886-39905	MsG0080048348.01.T01:CDS	50.0%
!	CAACAAGGTTGCTTTGCAGG+AGG	+	contig27end:39757-39776	MsG0080048348.01.T01:CDS	50.0%
!	TCTAGAATTGCAGGTCCACC+AGG	-	contig27end:40187-40206	None:intergenic	50.0%
!	TTTTGGATTGCACACCCTGG+TGG	+	contig27end:40169-40188	MsG0080048348.01.T01:CDS	50.0%
!	TTTTGTTGGAGCTGGCAGGT+GGG	-	contig27end:36710-36729	None:intergenic	50.0%
!!	AGGGTGAAGGAAGAGTGTTG+TGG	-	contig27end:39023-39042	None:intergenic	50.0%
!!	CAACAAAGGTGCTCGTGTGT+TGG	+	contig27end:39816-39835	MsG0080048348.01.T01:CDS	50.0%
!!	GCCCCCATTTGGTGTTTGAA+TGG	-	contig27end:37726-37745	None:intergenic	50.0%
!!	GTGAAGGAAGAGTGTTGTGG+TGG	-	contig27end:39020-39039	None:intergenic	50.0%
!!	GTGAGTTGGTAATCAGCTCC+AGG	-	contig27end:39691-39710	None:intergenic	50.0%
!!	TCAGGATGGACTGAAGACCA+CGG	+	contig27end:40334-40353	MsG0080048348.01.T01:CDS	50.0%
!!	TGGTTTTTGGGAGTTGCGGT+TGG	-	contig27end:36627-36646	None:intergenic	50.0%
	AATGGGAGCCGACAACCTCT+AGG	+	contig27end:38158-38177	MsG0080048348.01.T01:intron	55.0%
	ACTGAATCCTTGCAGGGCCA+AGG	-	contig27end:37542-37561	None:intergenic	55.0%
	ATTCTTCCCTTGGCCCTGCA+AGG	+	contig27end:37532-37551	MsG0080048348.01.T01:intron	55.0%
	CGCAATGCTCAAAGGGCAGA+AGG	+	contig27end:39134-39153	MsG0080048348.01.T01:intron	55.0%
	CGGCCCTAGTGATACTCACT+TGG	+	contig27end:39873-39892	MsG0080048348.01.T01:CDS	55.0%
	CTGAATCCTTGCAGGGCCAA+GGG	-	contig27end:37541-37560	None:intergenic	55.0%
	GATGGTCACCTTCGTGAAGC+TGG	+	contig27end:40038-40057	MsG0080048348.01.T01:intron	55.0%
	GCAGCCTCCTTCCCTAATCT+AGG	-	contig27end:39586-39605	None:intergenic	55.0%
	GGATGCTAGGCAAGACATGG+TGG	+	contig27end:39550-39569	MsG0080048348.01.T01:intron	55.0%
	GGCCTGGAGCTTTCTCTGAA+AGG	+	contig27end:37625-37644	MsG0080048348.01.T01:intron	55.0%
	GGTCTTCAGTCCATCCTGAG+CGG	-	contig27end:40333-40352	None:intergenic	55.0%
	GTATTGGGCCAAGAGTGGTG+TGG	+	contig27end:37310-37329	MsG0080048348.01.T01:intron	55.0%
!	AGGTTGCTTTGCAGGAGGCA+CGG	+	contig27end:39762-39781	MsG0080048348.01.T01:CDS	55.0%
!	CGATGCTTGGAGCATCTTCC+AGG	-	contig27end:38017-38036	None:intergenic	55.0%
!	GGCAAGACATGGTGGTGTAG+AGG	+	contig27end:39558-39577	MsG0080048348.01.T01:intron	55.0%
!	TTCAACAATGGCTGGCTGGC+TGG	-	contig27end:38898-38917	None:intergenic	55.0%
!	TTTTGCGTGTTGGAGTGGCG+AGG	-	contig27end:36972-36991	None:intergenic	55.0%
!!	ATTGGTGACGTCGCTTACGG+TGG	-	contig27end:35828-35847	None:intergenic	55.0%
!!	CAGGATGGACTGAAGACCAC+GGG	+	contig27end:40335-40354	MsG0080048348.01.T01:CDS	55.0%
!!	GGTGTTTTGTTGGAGCTGGC+AGG	-	contig27end:36714-36733	None:intergenic	55.0%
!!	GTTTTGTTGGAGCTGGCAGG+TGG	-	contig27end:36711-36730	None:intergenic	55.0%
	GGACTGAAGACCACGGGAGA+AGG	+	contig27end:40341-40360	MsG0080048348.01.T01:CDS	60.0%
	GGAGTGGCGAGGTTAACTCG+AGG	-	contig27end:36961-36980	None:intergenic	60.0%
	GGGCCAAGAGTGGTGTGGAT+AGG	+	contig27end:37315-37334	MsG0080048348.01.T01:intron	60.0%
!	AGGAGGCACGGTGCTTCGTT+TGG	+	contig27end:39774-39793	MsG0080048348.01.T01:CDS	60.0%
!!	GCAGGGTATTGGGCCAAGAG+TGG	+	contig27end:37305-37324	MsG0080048348.01.T01:intron	60.0%
!!	GGGCGGTGTTTTGTTGGAGC+TGG	-	contig27end:36718-36737	None:intergenic	60.0%
!!	GGTTGTCGGCTCCCATTGCT+TGG	-	contig27end:38155-38174	None:intergenic	60.0%
!	GGCTGGCTGGCTGGCTAGAT+GGG	-	contig27end:38889-38908	None:intergenic	65.0%
!	TGGCTGGCTGGCTGGCTAGA+TGG	-	contig27end:38890-38909	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
contig27end	gene	35727	40369	35727	ID=MsG0080048348.01;Name=MsG0080048348.01
contig27end	mRNA	35727	40369	35727	ID=MsG0080048348.01.T01;Parent=MsG0080048348.01;Name=MsG0080048348.01.T01;_AED=0.49;_eAED=0.63;_QI=0\|0\|0\|1\|1\|1\|3\|0\|307
contig27end	exon	35727	35963	35727	ID=MsG0080048348.01.T01:exon:22004;Parent=MsG0080048348.01.T01
contig27end	exon	39559	39959	39559	ID=MsG0080048348.01.T01:exon:22003;Parent=MsG0080048348.01.T01
contig27end	exon	40084	40369	40084	ID=MsG0080048348.01.T01:exon:22002;Parent=MsG0080048348.01.T01
contig27end	CDS	35727	35963	35727	ID=MsG0080048348.01.T01:cds;Parent=MsG0080048348.01.T01
contig27end	CDS	39559	39959	39559	ID=MsG0080048348.01.T01:cds;Parent=MsG0080048348.01.T01
contig27end	CDS	40084	40369	40084	ID=MsG0080048348.01.T01:cds;Parent=MsG0080048348.01.T01

Gene Sequence

>MsG0080048348.01.T01

ATGAACCTAACCATTAATGATATCACCACTGCAATCTTCAAAACTATCCAATACTCTACCCTTAATGTTGCAAATGGTCTGCAACATGCATACAGAAGCCACCGTAAGCGACGTCACCAATCATTTGATGATGACGACGATGATGATGGTCGGCTTGAAGAAAAATCAGAGAGATTGGTTAGGCTTGAACACTTTTTCGAGAACATGCTGAAAAATAATGAGGCCAAGTTAATGCAGGCAAGACATGGTGGTGTAGAGGTACCTAGATTAGGGAAGGAGGCTGCAGTAAAAGCTATAAAAGAATGGGGTCAACCAAAGTCAAAGATTACTCACTTGATCGTTTGCACCACAAGTGGTGTAGACATGCCTGGAGCTGATTACCAACTCACAAAACTCTTAGGTCTTCGCCCATATGTGAAAAGGTATATGATGTATCAACAAGGTTGCTTTGCAGGAGGCACGGTGCTTCGTTTGGCTAAAGATTTGGCTGAGAACAACAAAGGTGCTCGTGTGTTGGTTGTTTGTTCTGAAGTCACTGCAGTTACATTTCGCGGCCCTAGTGATACTCACTTGGACAGCCTTGTTGGACAAGCACTATTTGGAGATGGAGCTGCTGCACTGATTGTTGTTCAGATCCAATGTTCCGGGATTGTTTCAAAGAACATCAATAAAGCATTGGTTGAGGCTTTTGAAACATTGGGAATTTCTGATTACAATTCAATCTTTTGGATTGCACACCCTGGTGGACCTGCAATTCTAGATCAAGTAGAGCAAAAGTTAGCCTTAAAGCCTGAAAAGATGAAAGCCACCAGAGAAGTGCTCAGTGAATATGGAAATATGTCAAGTGCTTGTGTTTTGTTTATCTTAGATGAAATGAAAGAAGTCCGCTCAGGATGGACTGAAGACCACGGGAGAAGGACTTGA

Protein sequence

>MsG0080048348.01.T01

MNLTINDITTAIFKTIQYSTLNVANGLQHAYRSHRKRRHQSFDDDDDDDGRLEEKSERLVRLEHFFENMLKNNEAKLMQARHGGVEVPRLGKEAAVKAIKEWGQPKSKITHLIVCTTSGVDMPGADYQLTKLLGLRPYVKRYMMYQQGCFAGGTVLRLAKDLAENNKGARVLVVCSEVTAVTFRGPSDTHLDSLVGQALFGDGAAALIVVQIQCSGIVSKNINKALVEAFETLGISDYNSIFWIAHPGGPAILDQVEQKLALKPEKMKATREVLSEYGNMSSACVLFILDEMKEVRSGWTEDHGRRT*