AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0080048529.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080048529.01.T01 MTR_4g083070 95.876 194 8 0 51 244 1 194 3.12e-105 303
MsG0080048529.01.T01 MTR_5g044530 52.590 251 59 7 51 241 1 251 1.09e-48 161
MsG0080048529.01.T01 MTR_4g109490 59.551 89 34 2 67 155 20 106 7.24e-31 114
MsG0080048529.01.T01 MTR_8g085390 45.833 120 63 2 49 167 7 125 5.75e-29 108
MsG0080048529.01.T01 MTR_5g097420 43.750 112 56 3 54 159 8 118 8.67e-22 87.4
MsG0080048529.01.T01 MTR_5g078200 43.434 99 54 2 67 164 356 453 8.34e-21 91.3
MsG0080048529.01.T01 MTR_8g085720 41.739 115 62 3 64 174 357 470 9.06e-21 90.9
MsG0080048529.01.T01 MTR_5g078200 43.434 99 54 2 67 164 323 420 1.11e-20 90.9
MsG0080048529.01.T01 MTR_4g132280 48.315 89 44 2 71 158 382 469 1.50e-20 90.5
MsG0080048529.01.T01 MTR_3g065460 40.870 115 58 3 61 166 343 456 1.68e-20 90.1
MsG0080048529.01.T01 MTR_8g089400 46.078 102 49 4 71 168 37 136 2.11e-20 85.1
MsG0080048529.01.T01 MTR_8g089400 46.078 102 49 4 71 168 57 156 3.39e-20 85.1
MsG0080048529.01.T01 MTR_1g116870 48.052 77 40 0 64 140 269 345 6.53e-20 87.8
MsG0080048529.01.T01 MTR_8g085400 39.806 103 59 1 46 148 8 107 7.03e-20 82.4
MsG0080048529.01.T01 MTR_3g082080 48.750 80 41 0 69 148 155 234 2.39e-19 84.3
MsG0080048529.01.T01 MTR_1g031900 43.000 100 55 2 71 169 361 459 9.65e-19 85.1
MsG0080048529.01.T01 MTR_8g085440 37.391 115 69 2 44 156 3 116 1.99e-18 78.6
MsG0080048529.01.T01 MTR_4g083500 35.849 106 66 2 71 176 402 505 2.63e-18 84.0
MsG0080048529.01.T01 MTR_3g083580 42.391 92 51 2 65 155 356 446 2.95e-18 83.6
MsG0080048529.01.T01 MTR_3g116510 52.703 74 35 0 67 140 233 306 4.04e-18 82.4
MsG0080048529.01.T01 MTR_3g116510 52.703 74 35 0 67 140 367 440 4.66e-18 83.2
MsG0080048529.01.T01 MTR_1g056640 40.816 98 56 2 61 157 396 492 7.44e-18 82.4
MsG0080048529.01.T01 MTR_4g133735 38.655 119 69 3 42 159 1 116 7.71e-18 77.0
MsG0080048529.01.T01 MTR_1g031900 45.122 82 44 1 71 151 361 442 1.03e-17 82.0
MsG0080048529.01.T01 MTR_1g084820 52.857 70 32 1 71 140 420 488 2.50e-17 81.3
MsG0080048529.01.T01 MTR_1g084820 48.101 79 40 1 71 149 420 497 3.07e-17 80.9
MsG0080048529.01.T01 MTR_8g085420 35.514 107 67 1 44 148 3 109 1.29e-16 74.3
MsG0080048529.01.T01 MTR_4g133730 36.697 109 65 2 47 151 1 109 3.05e-16 72.8
MsG0080048529.01.T01 MTR_4g069940 44.318 88 47 2 71 157 375 461 3.13e-16 77.8
MsG0080048529.01.T01 MTR_4g079530 41.573 89 50 2 70 157 36 123 4.65e-16 73.9
MsG0080048529.01.T01 MTR_2g049670 49.296 71 35 1 71 141 158 227 6.27e-16 76.3
MsG0080048529.01.T01 MTR_7g111600 44.828 87 45 3 71 156 160 244 8.65e-16 75.5
MsG0080048529.01.T01 MTR_7g111600 44.828 87 45 3 71 156 160 244 9.05e-16 74.7
MsG0080048529.01.T01 MTR_4g079530 41.573 89 50 2 70 157 36 123 1.01e-15 71.6
MsG0080048529.01.T01 MTR_3g048280 46.512 86 44 2 67 151 108 192 1.40e-15 73.2
MsG0080048529.01.T01 MTR_7g111600 44.828 87 45 3 71 156 160 244 1.91e-15 74.7
MsG0080048529.01.T01 MTR_5g015720 45.679 81 42 2 70 148 328 408 2.52e-15 75.1
MsG0080048529.01.T01 MTR_5g085580 40.909 88 50 2 71 157 382 468 4.08e-15 74.3
MsG0080048529.01.T01 MTR_1g022160 41.111 90 52 1 66 155 386 474 9.76e-15 73.6
MsG0080048529.01.T01 MTR_1g022160 40.659 91 46 1 66 148 386 476 2.40e-14 72.4
MsG0080048529.01.T01 MTR_1g022160 44.000 75 42 0 66 140 386 460 4.20e-14 71.6
MsG0080048529.01.T01 MTR_5g091650 37.097 124 58 4 71 176 108 229 4.59e-14 70.1
MsG0080048529.01.T01 MTR_5g091650 43.820 89 47 3 71 158 108 194 1.73e-13 67.4
MsG0080048529.01.T01 MTR_8g102340 33.684 95 61 2 62 155 325 418 1.74e-13 69.7
MsG0080048529.01.T01 MTR_6g088885 35.632 87 53 2 71 155 369 454 8.91e-13 67.8
MsG0080048529.01.T01 MTR_8g012400 37.500 88 53 2 71 157 374 460 1.16e-11 64.3
MsG0080048529.01.T01 MTR_8g012400 37.500 88 53 2 71 157 294 380 1.59e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080048529.01.T01 AT1G18650 54.630 108 48 1 51 158 1 107 8.08e-39 132
MsG0080048529.01.T01 AT1G18650 54.630 108 48 1 51 158 1 107 1.75e-38 132
MsG0080048529.01.T01 AT2G03505 57.143 98 41 1 64 160 14 111 3.99e-37 127
MsG0080048529.01.T01 AT1G69295 56.731 104 44 1 51 154 1 103 3.85e-36 127
MsG0080048529.01.T01 AT2G03505 56.701 97 41 1 64 159 14 110 1.26e-35 125
MsG0080048529.01.T01 AT2G03505 56.701 97 41 1 64 159 14 110 1.26e-35 125
MsG0080048529.01.T01 AT2G03505 56.701 97 41 1 64 159 14 110 1.26e-35 125
MsG0080048529.01.T01 AT4G13600 44.578 166 90 2 15 179 5 169 3.41e-34 123
MsG0080048529.01.T01 AT1G69295 57.000 100 42 1 55 154 1 99 7.22e-33 119
MsG0080048529.01.T01 AT5G61130 40.884 181 93 4 65 236 15 190 5.71e-32 116
MsG0080048529.01.T01 AT1G13830 53.409 88 40 1 69 155 33 120 5.77e-29 108
MsG0080048529.01.T01 AT1G13830 53.409 88 40 1 69 155 19 106 7.54e-29 108
MsG0080048529.01.T01 AT5G08000 52.128 94 44 1 63 156 13 105 5.98e-28 105
MsG0080048529.01.T01 AT1G26450 40.426 188 83 6 51 221 1 176 8.50e-28 105
MsG0080048529.01.T01 AT5G08000 38.068 176 86 4 39 195 10 181 1.17e-27 105
MsG0080048529.01.T01 AT1G11820 48.315 89 44 2 71 158 381 468 3.54e-21 92.4
MsG0080048529.01.T01 AT1G79480 50.000 72 36 0 67 138 306 377 9.90e-20 87.4
MsG0080048529.01.T01 AT1G79480 50.000 72 36 0 67 138 307 378 1.04e-19 87.4
MsG0080048529.01.T01 AT2G01630 43.820 89 48 2 71 158 360 447 1.42e-19 87.8
MsG0080048529.01.T01 AT1G11820 49.367 79 39 1 71 148 381 459 2.02e-19 87.0
MsG0080048529.01.T01 AT5G35740 45.263 95 49 3 67 159 26 119 2.76e-19 80.9
MsG0080048529.01.T01 AT1G66250 36.220 127 78 3 71 196 368 492 8.79e-19 85.5
MsG0080048529.01.T01 AT2G01630 44.304 79 43 1 71 148 360 438 2.81e-18 83.6
MsG0080048529.01.T01 AT2G30933 44.944 89 46 3 71 158 82 168 1.04e-17 79.7
MsG0080048529.01.T01 AT2G05790 42.268 97 51 3 62 157 378 470 1.17e-17 82.0
MsG0080048529.01.T01 AT5G63240 33.333 123 70 3 46 157 7 128 1.24e-17 77.0
MsG0080048529.01.T01 AT4G26830 40.860 93 53 2 66 157 366 457 1.44e-17 81.6
MsG0080048529.01.T01 AT4G26830 40.860 93 53 2 66 157 362 453 1.79e-17 81.3
MsG0080048529.01.T01 AT5G24318 48.649 74 38 0 63 136 364 437 2.09e-17 81.3
MsG0080048529.01.T01 AT5G24318 48.649 74 38 0 63 136 225 298 2.18e-17 80.1
MsG0080048529.01.T01 AT5G24318 48.649 74 38 0 63 136 364 437 2.67e-17 80.9
MsG0080048529.01.T01 AT2G16230 40.698 86 50 1 71 156 366 450 4.05e-17 80.5
MsG0080048529.01.T01 AT1G09460 48.235 85 41 3 71 155 137 218 4.38e-17 79.3
MsG0080048529.01.T01 AT4G05430 37.895 95 57 2 71 164 23 116 4.54e-17 75.9
MsG0080048529.01.T01 AT2G04910 41.667 96 53 2 57 151 3 96 4.75e-17 74.7
MsG0080048529.01.T01 AT2G04910 46.835 79 41 1 71 148 15 93 6.71e-17 74.7
MsG0080048529.01.T01 AT4G05430 37.895 95 57 2 71 164 48 141 7.56e-17 75.9
MsG0080048529.01.T01 AT3G58100 41.304 92 52 2 70 160 40 130 1.03e-16 75.9
MsG0080048529.01.T01 AT2G30933 45.570 79 41 2 71 148 82 159 1.13e-16 75.1
MsG0080048529.01.T01 AT2G30933 45.570 79 41 2 71 148 82 159 1.13e-16 75.1
MsG0080048529.01.T01 AT3G58100 41.304 92 52 2 70 160 116 206 1.77e-16 76.6
MsG0080048529.01.T01 AT4G05430 40.506 79 46 1 71 148 48 126 3.67e-16 73.2
MsG0080048529.01.T01 AT5G55180 39.175 97 54 3 62 157 366 458 3.90e-16 77.4
MsG0080048529.01.T01 AT5G55180 40.909 88 48 2 62 148 366 450 4.58e-16 77.4
MsG0080048529.01.T01 AT4G29360 33.071 127 78 3 71 196 401 521 8.43e-16 76.6
MsG0080048529.01.T01 AT4G34480 40.698 86 50 1 71 156 253 337 8.57e-16 76.3
MsG0080048529.01.T01 AT1G29380 46.341 82 42 2 71 151 148 228 9.17e-16 74.3
MsG0080048529.01.T01 AT1G29380 46.341 82 42 2 71 151 148 228 9.17e-16 74.3
MsG0080048529.01.T01 AT4G34480 40.698 86 50 1 71 156 364 448 9.78e-16 76.3
MsG0080048529.01.T01 AT4G29360 38.298 94 56 2 71 163 391 483 1.02e-15 76.3
MsG0080048529.01.T01 AT1G29380 46.154 91 46 3 71 160 148 236 1.12e-15 74.7
MsG0080048529.01.T01 AT5G67460 43.750 80 44 1 70 148 292 371 1.14e-15 75.9
MsG0080048529.01.T01 AT5G67460 43.750 80 44 1 70 148 292 371 1.20e-15 75.9
MsG0080048529.01.T01 AT1G29380 46.341 82 42 2 71 151 148 228 1.31e-15 75.1
MsG0080048529.01.T01 AT5G63225 35.955 89 56 1 67 155 21 108 1.61e-15 70.9
MsG0080048529.01.T01 AT5G63230 38.824 85 51 1 71 155 26 109 9.08e-15 68.9
MsG0080048529.01.T01 AT3G55430 38.235 68 42 0 71 138 366 433 9.76e-15 73.6
MsG0080048529.01.T01 AT4G09462 34.906 106 66 2 54 157 10 114 1.13e-14 68.6
MsG0080048529.01.T01 AT4G09465 34.259 108 66 2 54 157 10 116 1.37e-14 68.6
MsG0080048529.01.T01 AT4G09467 34.259 108 66 2 54 157 10 116 1.87e-14 68.2
MsG0080048529.01.T01 AT4G09464 34.259 108 66 2 54 157 10 116 1.87e-14 68.2
MsG0080048529.01.T01 AT4G09466 34.259 108 66 2 54 157 10 116 2.08e-14 68.2
MsG0080048529.01.T01 AT1G66852 35.514 107 65 4 51 155 5 109 4.54e-14 67.0
MsG0080048529.01.T01 AT1G66870 34.862 109 66 4 49 155 5 110 9.30e-14 66.2
MsG0080048529.01.T01 AT5G63250 32.039 103 66 2 58 157 29 130 1.02e-13 66.6
MsG0080048529.01.T01 AT4G16165 39.048 105 59 4 55 155 7 110 1.06e-13 65.9
MsG0080048529.01.T01 AT1G66870 32.075 106 70 2 51 155 33 137 1.61e-13 66.2
MsG0080048529.01.T01 AT2G39640 38.462 91 55 1 68 158 457 546 1.64e-13 70.1
MsG0080048529.01.T01 AT2G39640 38.372 86 52 1 71 156 359 443 7.99e-11 62.0
MsG0080048529.01.T01 AT5G56590 34.783 92 58 2 71 161 369 459 2.60e-13 69.3
MsG0080048529.01.T01 AT2G29735 36.667 90 51 2 53 136 7 96 3.08e-13 64.7
MsG0080048529.01.T01 AT4G09090 34.483 87 53 1 54 136 10 96 5.34e-13 64.3
MsG0080048529.01.T01 AT3G13560 40.541 74 43 1 68 140 359 432 7.44e-13 67.8
MsG0080048529.01.T01 AT3G13560 40.541 74 43 1 68 140 359 432 7.44e-13 67.8
MsG0080048529.01.T01 AT3G13560 40.541 74 43 1 68 140 359 432 7.44e-13 67.8
MsG0080048529.01.T01 AT3G13560 40.541 74 43 1 68 140 359 432 7.44e-13 67.8
MsG0080048529.01.T01 AT3G13560 40.541 74 43 1 68 140 359 432 7.44e-13 67.8
MsG0080048529.01.T01 AT1G66855 33.000 100 61 3 55 148 5 104 4.93e-11 58.9

Find 74 sgRNAs with CRISPR-Local

Find 155 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTAAAGGAAGAAAACAAAAT+TGG 0.218637 contig347end:+17668 None:intergenic
TCCAGTTCCGGTTCCGGTTC+CGG 0.245908 contig347end:+17995 None:intergenic
TGTAGCCCTATTCTTCAAAA+TGG 0.254583 contig347end:-19285 MsG0080048529.01.T01:CDS
TTTCTTCCCAAACTCGCTTA+TGG 0.274551 contig347end:+19833 None:intergenic
TCCGGTTCCGGTTCCGGTTC+CGG 0.283594 contig347end:+18007 None:intergenic
TACCATGTGTTTAGCCTTCA+AGG 0.290004 contig347end:-17826 MsG0080048529.01.T01:CDS
TCAAAAGCCATAAGCGAGTT+TGG 0.291289 contig347end:-19840 MsG0080048529.01.T01:CDS
TCCGGTTCCGGTTCCGGTTC+CGG 0.293820 contig347end:+18001 None:intergenic
ACCGGAACCGGAACCGGAAC+CGG 0.296793 contig347end:-18002 MsG0080048529.01.T01:CDS
ACCGGAACCGGAACCGGAAC+CGG 0.312906 contig347end:-18008 MsG0080048529.01.T01:CDS
ACCGGAACCGGAACCGGAAC+TGG 0.319370 contig347end:-17996 MsG0080048529.01.T01:CDS
GCCTATTCCAGTTCCGGTTC+CGG 0.327107 contig347end:+17989 None:intergenic
AATGCTACTACTAGTGAATT+AGG 0.335608 contig347end:-17788 MsG0080048529.01.T01:three_prime_UTR
GCCTGTGCCTATTCCAGTTC+CGG 0.341051 contig347end:+17983 None:intergenic
CAGTGATCTTTCACAGTGTT+GGG 0.344828 contig347end:+19249 None:intergenic
CCGGGCACCGGAACCGGAAC+CGG 0.348279 contig347end:-18014 MsG0080048529.01.T01:CDS
CAATTTGGTTTCTTGTTTAG+TGG 0.354923 contig347end:-17627 MsG0080048529.01.T01:three_prime_UTR
TTACTTGGTGGTGTTTGAAT+AGG 0.373556 contig347end:+19150 None:intergenic
TCAACTCCGGGCACCGGAAC+CGG 0.380673 contig347end:-18020 MsG0080048529.01.T01:CDS
CTTGAACATTACCCTTTCTC+TGG 0.398968 contig347end:+19202 None:intergenic
TACCTTAGTTTAGACCTTGA+AGG 0.407311 contig347end:+17812 None:intergenic
CCGGTTCCGGTTCCGGTGCC+CGG 0.408290 contig347end:+18014 None:intergenic
GCGGGTAGTCCTACTGTGTT+TGG 0.412317 contig347end:-17942 MsG0080048529.01.T01:CDS
TAAAGGAAGAAAACAAAATT+GGG 0.412898 contig347end:+17669 None:intergenic
AACAAGGGACAATAGCAATA+TGG 0.413054 contig347end:+17851 None:intergenic
ATTGATTATGCTTGTGGAGC+TGG 0.416308 contig347end:-19315 MsG0080048529.01.T01:CDS
AACTCCATCAACTGGTTCAC+CGG 0.420333 contig347end:-18045 MsG0080048529.01.T01:CDS
AGTCCATCATCTACAATTGA+TGG 0.428014 contig347end:-17915 MsG0080048529.01.T01:CDS
CAAAAGCCATAAGCGAGTTT+GGG 0.436603 contig347end:-19839 MsG0080048529.01.T01:CDS
AAAACATACCCTGGACTTGA+AGG 0.436702 contig347end:+18810 None:intergenic
AAGTAGAAGTAGAACTTCAT+TGG 0.441606 contig347end:-17572 MsG0080048529.01.T01:three_prime_UTR
ACCGGAACCGGAACTGGAAT+AGG 0.452768 contig347end:-17990 MsG0080048529.01.T01:CDS
TGGCTTTCGTGCAGCAATGC+AGG 0.454054 contig347end:-18068 MsG0080048529.01.T01:intron
GGTTCACCGGGTTCAACTCC+GGG 0.460627 contig347end:-18032 MsG0080048529.01.T01:CDS
ACAGTGATCTTTCACAGTGT+TGG 0.461006 contig347end:+19248 None:intergenic
AGGACTACCCGCAGTTGATC+CGG 0.472219 contig347end:+17953 None:intergenic
TGTGTTTACCCTTCAAGTCC+AGG 0.474473 contig347end:-18819 MsG0080048529.01.T01:CDS
AGCCTTCAAGGTCTAAACTA+AGG 0.476689 contig347end:-17814 MsG0080048529.01.T01:exon
CAGAGAAAGGGTAATGTTCA+AGG 0.477756 contig347end:-19201 MsG0080048529.01.T01:CDS
TGTATATCTAAGATTGTGAC+TGG 0.478916 contig347end:-17526 MsG0080048529.01.T01:three_prime_UTR
TGGTTCACCGGGTTCAACTC+CGG 0.485987 contig347end:-18033 MsG0080048529.01.T01:CDS
TAATAGCTATTACCAGAGAA+AGG 0.488351 contig347end:-19214 MsG0080048529.01.T01:CDS
AAAATCACTTTCTCTTGCCA+TGG 0.488886 contig347end:-19736 MsG0080048529.01.T01:CDS
TTGAGTGACCAGTGAGGGAC+AGG 0.498202 contig347end:+19688 None:intergenic
TTATATTGTGTATGCAAAGA+TGG 0.499671 contig347end:-19366 MsG0080048529.01.T01:CDS
AATGCAGGAACTCCATCAAC+TGG 0.504129 contig347end:-18053 MsG0080048529.01.T01:CDS
TTGCCATCAATTGTAGATGA+TGG 0.504668 contig347end:+17912 None:intergenic
TTAGATATACAAATGAGAAA+AGG 0.513190 contig347end:+17538 None:intergenic
ATTGATGGCAACTCTGCAGC+TGG 0.521396 contig347end:-17900 MsG0080048529.01.T01:CDS
AAGGCAATTGATTATGCTTG+TGG 0.523668 contig347end:-19321 MsG0080048529.01.T01:CDS
ACAGTGTTGGGTTGAAAACA+AGG 0.524161 contig347end:+19261 None:intergenic
GAACCCGGTGAACCAGTTGA+TGG 0.531141 contig347end:+18041 None:intergenic
AATAGCTATTACCAGAGAAA+GGG 0.544423 contig347end:-19213 MsG0080048529.01.T01:CDS
TGAAGTGAATGAGATGTTTG+AGG 0.544946 contig347end:+19758 None:intergenic
CCGGGTTCAACTCCGGGCAC+CGG 0.548439 contig347end:-18026 MsG0080048529.01.T01:CDS
CATAATCAATTGCCTTCTGA+AGG 0.556126 contig347end:+19328 None:intergenic
CTAAACACATGGTAATAACA+AGG 0.561660 contig347end:+17835 None:intergenic
ACAGGCACCGGATCAACTGC+GGG 0.564463 contig347end:-17960 MsG0080048529.01.T01:CDS
CCGGTGCCCGGAGTTGAACC+CGG 0.572383 contig347end:+18026 None:intergenic
GGACAATAGCAATATGGTGT+TGG 0.583426 contig347end:+17857 None:intergenic
ATAGGCACAGGCACAGGCAC+CGG 0.590562 contig347end:-17972 MsG0080048529.01.T01:CDS
ATCTAGTCAACTCCTTCAGA+AGG 0.591377 contig347end:-19340 MsG0080048529.01.T01:CDS
GTGTTTACCCTTCAAGTCCA+GGG 0.598260 contig347end:-18818 MsG0080048529.01.T01:intron
ACTGGAATAGGCACAGGCAC+AGG 0.601409 contig347end:-17978 MsG0080048529.01.T01:CDS
ACCGGAACTGGAATAGGCAC+AGG 0.603632 contig347end:-17984 MsG0080048529.01.T01:CDS
AAACATACCCTGGACTTGAA+GGG 0.606188 contig347end:+18811 None:intergenic
CACAGGCACCGGATCAACTG+CGG 0.606666 contig347end:-17961 MsG0080048529.01.T01:CDS
GACCTTGAAGGCTAAACACA+TGG 0.610351 contig347end:+17824 None:intergenic
AAAATAGAGCAGAAAAGCCA+TGG 0.611716 contig347end:+19719 None:intergenic
ACTCCATCAACTGGTTCACC+GGG 0.624481 contig347end:-18044 MsG0080048529.01.T01:CDS
GGACTTATTCCAAACACAGT+AGG 0.629153 contig347end:+17933 None:intergenic
TAAACACATGGTAATAACAA+GGG 0.671673 contig347end:+17836 None:intergenic
CTTACTTGAGTGACCAGTGA+GGG 0.700235 contig347end:+19683 None:intergenic
GCTTACTTGAGTGACCAGTG+AGG 0.792478 contig347end:+19682 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GTTTCTTAAAAAAAAAAAAA+AGG + contig347end:17877-17896 None:intergenic 10.0%
!!! ATATTTTGAATATGATAAAA+TGG - contig347end:19772-19791 MsG0080048529.01.T01:CDS 10.0%
!!! TATTTTGAATATGATAAAAT+GGG - contig347end:19773-19792 MsG0080048529.01.T01:CDS 10.0%
!!! TTTTTTTTTTTTAAGAAACA+GGG - contig347end:17878-17897 MsG0080048529.01.T01:CDS 10.0%
!!! TTTTTTTTTTTTTAAGAAAC+AGG - contig347end:17877-17896 MsG0080048529.01.T01:CDS 10.0%
!! GAAAATAATATTTCATACAA+TGG + contig347end:17853-17872 None:intergenic 15.0%
!! TTACTATTATTCTATGAAAA+AGG - contig347end:17924-17943 MsG0080048529.01.T01:CDS 15.0%
!!! ACAAATTATTTTTGTTGAAT+AGG - contig347end:17979-17998 MsG0080048529.01.T01:CDS 15.0%
!!! ATTTTGAATATGATAAAATG+GGG - contig347end:19774-19793 MsG0080048529.01.T01:CDS 15.0%
!!! TAACTTAAAATACAATTTTC+AGG + contig347end:18774-18793 None:intergenic 15.0%
!!! TACTTAATTTTTCAACTTAA+AGG + contig347end:19723-19742 None:intergenic 15.0%
!!! TGATTTTCTCAAATTATTAT+TGG + contig347end:18323-18342 None:intergenic 15.0%
!!! TTTAAGTTGAAAAATTAAGT+AGG - contig347end:19722-19741 MsG0080048529.01.T01:CDS 15.0%
!!! TTTTTTTTTTTAAGAAACAG+GGG - contig347end:17879-17898 MsG0080048529.01.T01:CDS 15.0%
!! AACATCAGAGAAAATAAAAA+AGG + contig347end:17800-17819 None:intergenic 20.0%
!! ACAAAAATTACAGAAAATAG+AGG + contig347end:18496-18515 None:intergenic 20.0%
!! ATAAAATGCTTATCAGAAAT+AGG + contig347end:18670-18689 None:intergenic 20.0%
!! TAAAGGAAGAAAACAAAATT+GGG + contig347end:19706-19725 None:intergenic 20.0%
!! TTAAAGGAAGAAAACAAAAT+TGG + contig347end:19707-19726 None:intergenic 20.0%
!! TTAGATATACAAATGAGAAA+AGG + contig347end:19837-19856 None:intergenic 20.0%
!! TTAGTTAGATTAAGATTAGT+CGG + contig347end:19177-19196 None:intergenic 20.0%
!!! AGATGTTTATCAAACATTTT+AGG - contig347end:18436-18455 MsG0080048529.01.T01:intron 20.0%
!!! AGTGAATTAGGAAAATTTTA+GGG - contig347end:19596-19615 MsG0080048529.01.T01:intron 20.0%
!!! TAGTGAATTAGGAAAATTTT+AGG - contig347end:19595-19614 MsG0080048529.01.T01:intron 20.0%
!!! TATTTCTGATAAGCATTTTA+TGG - contig347end:18669-18688 MsG0080048529.01.T01:intron 20.0%
!!! TTTTTTATTTTCTCTGATGT+TGG - contig347end:17799-17818 MsG0080048529.01.T01:three_prime_UTR 20.0%
! AAAAGAACAACAAAGATCAT+AGG + contig347end:17827-17846 None:intergenic 25.0%
! AATACTGTCAAATGTTGTTA+TGG - contig347end:18933-18952 MsG0080048529.01.T01:intron 25.0%
! AATCTAACTAAATTATTGCG+TGG - contig347end:19186-19205 MsG0080048529.01.T01:CDS 25.0%
! ATTATGAAAAAAACATACCC+TGG + contig347end:18574-18593 None:intergenic 25.0%
! CTATTATTTCAAGAGAAAAG+AGG + contig347end:18992-19011 None:intergenic 25.0%
! GAAAAATTAAGTAGGCAATT+TGG - contig347end:19730-19749 MsG0080048529.01.T01:CDS 25.0%
! GTATATTTACCTATGAAGTA+TGG - contig347end:18259-18278 MsG0080048529.01.T01:intron 25.0%
! TAAACACATGGTAATAACAA+GGG + contig347end:19539-19558 None:intergenic 25.0%
! TACAGAAAATAGAGGAAAAT+CGG + contig347end:18488-18507 None:intergenic 25.0%
! TTATATTGTGTATGCAAAGA+TGG - contig347end:18006-18025 MsG0080048529.01.T01:CDS 25.0%
! TTCAATGTAATTATATGCGT+CGG - contig347end:18348-18367 MsG0080048529.01.T01:intron 25.0%
! TTTCATTGTGTGATTAGATT+TGG - contig347end:18800-18819 MsG0080048529.01.T01:intron 25.0%
!! CTATGAAAAAGGTGAAATTT+TGG - contig347end:17935-17954 MsG0080048529.01.T01:CDS 25.0%
!! CTATGTTGATCTTTTTATCT+TGG - contig347end:19284-19303 MsG0080048529.01.T01:CDS 25.0%
!! GTGAATTAGGAAAATTTTAG+GGG - contig347end:19597-19616 MsG0080048529.01.T01:intron 25.0%
!! TTTTCTCTTGAAATAATAGC+TGG - contig347end:18993-19012 MsG0080048529.01.T01:intron 25.0%
!!! CATAATAAAGAGTACTTACT+TGG + contig347end:18240-18259 None:intergenic 25.0%
!!! CATTTTAGGCATATTTTTGT+TGG - contig347end:18450-18469 MsG0080048529.01.T01:intron 25.0%
!!! TATTGATTGGTTTAACATCA+TGG - contig347end:18826-18845 MsG0080048529.01.T01:CDS 25.0%
!!! TTTTCAGGAATTGAAAAATC+TGG + contig347end:18759-18778 None:intergenic 25.0%
AAGTAGAAGTAGAACTTCAT+TGG - contig347end:19800-19819 MsG0080048529.01.T01:CDS 30.0%
AATAGCTATTACCAGAGAAA+GGG - contig347end:18159-18178 MsG0080048529.01.T01:intron 30.0%
AATGCTACTACTAGTGAATT+AGG - contig347end:19584-19603 MsG0080048529.01.T01:intron 30.0%
CTAAACACATGGTAATAACA+AGG + contig347end:19540-19559 None:intergenic 30.0%
GTAATTGCAAAATCGAGATT+GGG - contig347end:19085-19104 MsG0080048529.01.T01:intron 30.0%
TAATAGCTATTACCAGAGAA+AGG - contig347end:18158-18177 MsG0080048529.01.T01:intron 30.0%
TATGAAGTATGGATACAACA+AGG - contig347end:18270-18289 MsG0080048529.01.T01:intron 30.0%
TGATAAACATCTATGAAGCA+TGG + contig347end:18428-18447 None:intergenic 30.0%
TTGTTGTATCCATACTTCAT+AGG + contig347end:18271-18290 None:intergenic 30.0%
! ATATTTTCGATGTCTATGCT+CGG - contig347end:19026-19045 MsG0080048529.01.T01:intron 30.0%
! ATTTTGCACAACAACAAAGA+TGG - contig347end:19638-19657 MsG0080048529.01.T01:intron 30.0%
! CAATTTGGTTTCTTGTTTAG+TGG - contig347end:19745-19764 MsG0080048529.01.T01:CDS 30.0%
! TGTATATCTAAGATTGTGAC+TGG - contig347end:19846-19865 MsG0080048529.01.T01:CDS 30.0%
! TTCAAGAGAAAAGAGGATTT+TGG + contig347end:18985-19004 None:intergenic 30.0%
!! AAGGTCCATTTTGAAGAATA+GGG + contig347end:18095-18114 None:intergenic 30.0%
!! AATAAAGAGTACTTACTTGG+TGG + contig347end:18237-18256 None:intergenic 30.0%
!! TTAGATTTGGAGGTATTGAT+TGG - contig347end:18813-18832 MsG0080048529.01.T01:intron 30.0%
AAAATAGAGCAGAAAAGCCA+TGG + contig347end:17656-17675 None:intergenic 35.0%
AAAATCACTTTCTCTTGCCA+TGG - contig347end:17636-17655 MsG0080048529.01.T01:three_prime_UTR 35.0%
AAACATCTATGAAGCATGGA+CGG + contig347end:18424-18443 None:intergenic 35.0%
AAATCTGGTGAAATCACCTT+TGG + contig347end:18744-18763 None:intergenic 35.0%
AACAAGGGACAATAGCAATA+TGG + contig347end:19524-19543 None:intergenic 35.0%
AAGGCAATTGATTATGCTTG+TGG - contig347end:18051-18070 MsG0080048529.01.T01:CDS 35.0%
AGTCCATCATCTACAATTGA+TGG - contig347end:19457-19476 MsG0080048529.01.T01:intron 35.0%
CATAATCAATTGCCTTCTGA+AGG + contig347end:18047-18066 None:intergenic 35.0%
CATTGTGTGATTAGATTTGG+AGG - contig347end:18803-18822 MsG0080048529.01.T01:intron 35.0%
GGTAATTGCAAAATCGAGAT+TGG - contig347end:19084-19103 MsG0080048529.01.T01:intron 35.0%
TACCTTAGTTTAGACCTTGA+AGG + contig347end:19563-19582 None:intergenic 35.0%
TGAAGTGAATGAGATGTTTG+AGG + contig347end:17617-17636 None:intergenic 35.0%
TGTAGCCCTATTCTTCAAAA+TGG - contig347end:18087-18106 MsG0080048529.01.T01:intron 35.0%
TTGCCATCAATTGTAGATGA+TGG + contig347end:19463-19482 None:intergenic 35.0%
TTGGATCCATACATGGAAAA+AGG + contig347end:18725-18744 None:intergenic 35.0%
! ATTTTGCAATTACCAATGCG+TGG + contig347end:19078-19097 None:intergenic 35.0%
! TTCAGTCCTTTTTCCATGTA+TGG - contig347end:18716-18735 MsG0080048529.01.T01:intron 35.0%
! TTTTCCATGTATGGATCCAA+AGG - contig347end:18725-18744 MsG0080048529.01.T01:intron 35.0%
! TTTTCTGTTGAGTGTGAAGT+GGG - contig347end:18628-18647 MsG0080048529.01.T01:intron 35.0%
! TTTTTCTGTTGAGTGTGAAG+TGG - contig347end:18627-18646 MsG0080048529.01.T01:intron 35.0%
!! AGCATTTTATGGTTAGCTGT+TGG - contig347end:18680-18699 MsG0080048529.01.T01:intron 35.0%
!! CAAGGTCCATTTTGAAGAAT+AGG + contig347end:18096-18115 None:intergenic 35.0%
!! TTACTTGGTGGTGTTTGAAT+AGG + contig347end:18225-18244 None:intergenic 35.0%
AAAACATACCCTGGACTTGA+AGG + contig347end:18565-18584 None:intergenic 40.0%
AAACATACCCTGGACTTGAA+GGG + contig347end:18564-18583 None:intergenic 40.0%
ACAGTGATCTTTCACAGTGT+TGG + contig347end:18127-18146 None:intergenic 40.0%
AGCCTTCAAGGTCTAAACTA+AGG - contig347end:19558-19577 MsG0080048529.01.T01:intron 40.0%
ATCACCTTTGGATCCATACA+TGG + contig347end:18732-18751 None:intergenic 40.0%
ATCTAGTCAACTCCTTCAGA+AGG - contig347end:18032-18051 MsG0080048529.01.T01:CDS 40.0%
ATTGATTATGCTTGTGGAGC+TGG - contig347end:18057-18076 MsG0080048529.01.T01:CDS 40.0%
ATTGCAAAATCGAGATTGGG+CGG - contig347end:19088-19107 MsG0080048529.01.T01:intron 40.0%
CAAAAGCCATAAGCGAGTTT+GGG - contig347end:17533-17552 MsG0080048529.01.T01:three_prime_UTR 40.0%
CAGAGAAAGGGTAATGTTCA+AGG - contig347end:18171-18190 MsG0080048529.01.T01:intron 40.0%
CAGTGATCTTTCACAGTGTT+GGG + contig347end:18126-18145 None:intergenic 40.0%
CTTGAACATTACCCTTTCTC+TGG + contig347end:18173-18192 None:intergenic 40.0%
GGACAATAGCAATATGGTGT+TGG + contig347end:19518-19537 None:intergenic 40.0%
GGACTTATTCCAAACACAGT+AGG + contig347end:19442-19461 None:intergenic 40.0%
TCAAAAGCCATAAGCGAGTT+TGG - contig347end:17532-17551 MsG0080048529.01.T01:three_prime_UTR 40.0%
TTTCTTCCCAAACTCGCTTA+TGG + contig347end:17542-17561 None:intergenic 40.0%
! ATTGGGCGGTTTAGAATTCA+AGG - contig347end:19102-19121 MsG0080048529.01.T01:intron 40.0%
! CAAGGTAGTTGTGATTTTGC+TGG - contig347end:18189-18208 MsG0080048529.01.T01:intron 40.0%
! GTCGGTTTAGATATCAATGC+TGG + contig347end:19159-19178 None:intergenic 40.0%
! TACCATGTGTTTAGCCTTCA+AGG - contig347end:19546-19565 MsG0080048529.01.T01:intron 40.0%
!! ACAGTGTTGGGTTGAAAACA+AGG + contig347end:18114-18133 None:intergenic 40.0%
!! ATTTTAGGGGCTTTGATGTG+TGG - contig347end:19610-19629 MsG0080048529.01.T01:intron 40.0%
AATGCAGGAACTCCATCAAC+TGG - contig347end:19319-19338 MsG0080048529.01.T01:CDS 45.0%
AGTGTGAAGTGGGAATGTTG+TGG - contig347end:18638-18657 MsG0080048529.01.T01:intron 45.0%
CTTACTTGAGTGACCAGTGA+GGG + contig347end:17692-17711 None:intergenic 45.0%
GACCTTGAAGGCTAAACACA+TGG + contig347end:19551-19570 None:intergenic 45.0%
GTGTTTACCCTTCAAGTCCA+GGG - contig347end:18554-18573 MsG0080048529.01.T01:intron 45.0%
TCGATGTCTATGCTCGGATT+TGG - contig347end:19032-19051 MsG0080048529.01.T01:intron 45.0%
TGTGTTTACCCTTCAAGTCC+AGG - contig347end:18553-18572 MsG0080048529.01.T01:intron 45.0%
! AACTCCATCAACTGGTTCAC+CGG - contig347end:19327-19346 MsG0080048529.01.T01:CDS 45.0%
! GGCGGTTTAGAATTCAAGGT+TGG - contig347end:19106-19125 MsG0080048529.01.T01:intron 45.0%
! TTTTCTACAGCTGCAGCATC+TGG - contig347end:18529-18548 MsG0080048529.01.T01:intron 45.0%
CAAGGACACCAGTCACGATA+CGG - contig347end:18288-18307 MsG0080048529.01.T01:intron 50.0%
CAGTCAGCAATTCCACGCAT+TGG - contig347end:19063-19082 MsG0080048529.01.T01:intron 50.0%
GCTTACTTGAGTGACCAGTG+AGG + contig347end:17693-17712 None:intergenic 50.0%
! ACTCCATCAACTGGTTCACC+GGG - contig347end:19328-19347 MsG0080048529.01.T01:CDS 50.0%
! ATTGATGGCAACTCTGCAGC+TGG - contig347end:19472-19491 MsG0080048529.01.T01:intron 50.0%
!! TCCTCGGATTAGAGGTGTTC+CGG + contig347end:18397-18416 None:intergenic 50.0%
AAGCATGGACGGACACTCCT+CGG + contig347end:18413-18432 None:intergenic 55.0%
ACCGGAACACCTCTAATCCG+AGG - contig347end:18393-18412 MsG0080048529.01.T01:intron 55.0%
ACCGGAACCGGAACTGGAAT+AGG - contig347end:19382-19401 MsG0080048529.01.T01:intron 55.0%
ACCGGAACTGGAATAGGCAC+AGG - contig347end:19388-19407 MsG0080048529.01.T01:intron 55.0%
ACTGGAATAGGCACAGGCAC+AGG - contig347end:19394-19413 MsG0080048529.01.T01:intron 55.0%
AGGACTACCCGCAGTTGATC+CGG + contig347end:19422-19441 None:intergenic 55.0%
GAACCCGGTGAACCAGTTGA+TGG + contig347end:19334-19353 None:intergenic 55.0%
GCCTATTCCAGTTCCGGTTC+CGG + contig347end:19386-19405 None:intergenic 55.0%
GCCTGTGCCTATTCCAGTTC+CGG + contig347end:19392-19411 None:intergenic 55.0%
GCGGGTAGTCCTACTGTGTT+TGG - contig347end:19430-19449 MsG0080048529.01.T01:intron 55.0%
GTCAGTGTCCGTATCGTGAC+TGG + contig347end:18299-18318 None:intergenic 55.0%
TGGCTTTCGTGCAGCAATGC+AGG - contig347end:19304-19323 MsG0080048529.01.T01:CDS 55.0%
TGGTTCACCGGGTTCAACTC+CGG - contig347end:19339-19358 MsG0080048529.01.T01:CDS 55.0%
TTGAGTGACCAGTGAGGGAC+AGG + contig347end:17687-17706 None:intergenic 55.0%
! ACGGACACTCCTCGGATTAG+AGG + contig347end:18405-18424 None:intergenic 55.0%
!! CTGCTTTTCCTGTCCCTCAC+TGG - contig347end:17676-17695 MsG0080048529.01.T01:three_prime_UTR 55.0%
ACGCGACGTGTGTTCGACAC+CGG - contig347end:18375-18394 MsG0080048529.01.T01:intron 60.0%
ATAGGCACAGGCACAGGCAC+CGG - contig347end:19400-19419 MsG0080048529.01.T01:intron 60.0%
GGTTCACCGGGTTCAACTCC+GGG - contig347end:19340-19359 MsG0080048529.01.T01:CDS 60.0%
TCCAGTTCCGGTTCCGGTTC+CGG + contig347end:19380-19399 None:intergenic 60.0%
!! ACAGGCACCGGATCAACTGC+GGG - contig347end:19412-19431 MsG0080048529.01.T01:intron 60.0%
!! CACAGGCACCGGATCAACTG+CGG - contig347end:19411-19430 MsG0080048529.01.T01:intron 60.0%
ACCGGAACCGGAACCGGAAC+CGG - contig347end:19364-19383 MsG0080048529.01.T01:CDS 65.0%
ACCGGAACCGGAACCGGAAC+TGG - contig347end:19364-19383 MsG0080048529.01.T01:CDS 65.0%
TCCGGTTCCGGTTCCGGTTC+CGG + contig347end:19368-19387 None:intergenic 65.0%
! GGTGTCGAACACACGTCGCG+TGG + contig347end:18376-18395 None:intergenic 65.0%
!! TCAACTCCGGGCACCGGAAC+CGG - contig347end:19352-19371 MsG0080048529.01.T01:CDS 65.0%
CCGGGTTCAACTCCGGGCAC+CGG - contig347end:19346-19365 MsG0080048529.01.T01:CDS 70.0%
CCGGTGCCCGGAGTTGAACC+CGG + contig347end:19349-19368 None:intergenic 70.0%
CCGGTTCCGGTTCCGGTGCC+CGG + contig347end:19361-19380 None:intergenic 75.0%
!! CCGGGCACCGGAACCGGAAC+CGG - contig347end:19358-19377 MsG0080048529.01.T01:CDS 75.0%
Chromosome Type Strat End Strand Name
contig347end gene 17505 19889 17505 ID=MsG0080048529.01;Name=MsG0080048529.01
contig347end mRNA 17505 19889 17505 ID=MsG0080048529.01.T01;Parent=MsG0080048529.01;Name=MsG0080048529.01.T01;_AED=0.29;_eAED=0.38;_QI=0|1|0.75|1|0.66|0.75|4|316|244
contig347end exon 17505 18076 17505 ID=MsG0080048529.01.T01:exon:38039;Parent=MsG0080048529.01.T01
contig347end exon 18819 18855 18819 ID=MsG0080048529.01.T01:exon:38038;Parent=MsG0080048529.01.T01
contig347end exon 19154 19393 19154 ID=MsG0080048529.01.T01:exon:38037;Parent=MsG0080048529.01.T01
contig347end exon 19688 19889 19688 ID=MsG0080048529.01.T01:exon:38036;Parent=MsG0080048529.01.T01
contig347end CDS 19688 19889 19688 ID=MsG0080048529.01.T01:cds;Parent=MsG0080048529.01.T01
contig347end CDS 19154 19393 19154 ID=MsG0080048529.01.T01:cds;Parent=MsG0080048529.01.T01
contig347end CDS 18819 18855 18819 ID=MsG0080048529.01.T01:cds;Parent=MsG0080048529.01.T01
contig347end CDS 17821 18076 17821 ID=MsG0080048529.01.T01:cds;Parent=MsG0080048529.01.T01
contig347end three_prime_UTR 17505 17820 17505 ID=MsG0080048529.01.T01:three_prime_utr;Parent=MsG0080048529.01.T01
Gene Sequence

>MsG0080048529.01.T01

ATGTCAAGACTACTAGAGAGAAGAACGTCAAAAGCCATAAGCGAGTTTGGGAAGAAAAAAAAACGCTTGTTCTCTTCATCATCAATTCGTCTCTTTTTCTTTCACTCTGCCTCAAACATCTCATTCACTTCAAAATCACTTTCTCTTGCCATGGCTTTTCTGCTCTATTTTCTGCTTTTCCTGTCCCTCACTGGTCACTCAAGTGCTTTATATTGTGTATGCAAAGATGGTGTATCTAGTCAACTCCTTCAGAAGGCAATTGATTATGCTTGTGGAGCTGGAGCTGATTGTAGCCCTATTCTTCAAAATGGACCTTGTTTTCAACCCAACACTGTGAAAGATCACTGTAACTATGCTGTTAATAGCTATTACCAGAGAAAGGGTAATGTTCAAGGTAGTTGTGATTTTGCTGGAGCTGCAGCGCCTATTCAAACACCACCAACTGCAGCATCTGGATGTGTTTACCCTTCAAGTCCAGGCAATGCAGGAACTCCATCAACTGGTTCACCGGGTTCAACTCCGGGCACCGGAACCGGAACCGGAACCGGAACCGGAACTGGAATAGGCACAGGCACAGGCACCGGATCAACTGCGGGTAGTCCTACTGTGTTTGGAATAAGTCCATCATCTACAATTGATGGCAACTCTGCAGCTGGAGAGTCTTGTATGAAAAACACCAACACCATATTGCTATTGTCCCTTGTTATTACCATGTGTTTAGCCTTCAAGGTCTAA
Protein sequence

>MsG0080048529.01.T01

MSRLLERRTSKAISEFGKKKKRLFSSSSIRLFFFHSASNISFTSKSLSLAMAFLLYFLLFLSLTGHSSALYCVCKDGVSSQLLQKAIDYACGAGADCSPILQNGPCFQPNTVKDHCNYAVNSYYQRKGNVQGSCDFAGAAAPIQTPPTAASGCVYPSSPGNAGTPSTGSPGSTPGTGTGTGTGTGTGIGTGTGTGSTAGSPTVFGISPSSTIDGNSAAGESCMKNTNTILLLSLVITMCLAFKV*