Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048567.01.T01 | YP_009237639.1 | 100 | 181 | 0 | 0 | 2 | 182 | 42 | 222 | 1.39E-122 | 355 |
| MsG0080048567.01.T02 | YP_009237639.1 | 100 | 149 | 0 | 0 | 1 | 149 | 74 | 222 | 1.37E-97 | 290 |
| MsG0080048567.01.T03 | YP_009237639.1 | 100 | 72 | 0 | 0 | 1 | 72 | 151 | 222 | 2.73E-41 | 144 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048567.01.T01 | Q04654 | 91.304 | 184 | 13 | 1 | 2 | 182 | 101 | 284 | 5.20E-112 | 323 |
| MsG0080048567.01.T02 | P05499 | 91.275 | 149 | 13 | 0 | 1 | 149 | 236 | 384 | 1.12E-90 | 271 |
| MsG0080048567.01.T03 | P05499 | 90.278 | 72 | 7 | 0 | 1 | 72 | 313 | 384 | 4.52E-39 | 135 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048567.01.T02 | A0A126TH03 | 100.000 | 149 | 0 | 0 | 1 | 149 | 74 | 222 | 6.53e-98 | 290 |
| MsG0080048567.01.T03 | A0A126TH03 | 100.000 | 72 | 0 | 0 | 1 | 72 | 151 | 222 | 1.30e-41 | 144 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048008.01 | MsG0080048567.01 | 0.843581 | 1.282824e-58 | 2.016326e-55 |
| MsG0080048011.01 | MsG0080048567.01 | 0.837475 | 5.087075e-57 | 6.615676e-54 |
| MsG0080048567.01 | MsG0080049160.01 | 0.810470 | 1.134409e-50 | 6.843949e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048567.01.T01 | MTR_0248s0030 | 96.774 | 31 | 1 | 0 | 32 | 62 | 46 | 76 | 3.77e-13 | 63.2 |
| MsG0080048567.01.T02 | MTR_0248s0030 | 100.000 | 29 | 0 | 0 | 1 | 29 | 48 | 76 | 2.52e-12 | 60.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048567.01.T01 | ATMG01170 | 87.912 | 182 | 19 | 1 | 2 | 180 | 204 | 385 | 1.14e-106 | 311 |
| MsG0080048567.01.T01 | AT2G07741 | 87.912 | 182 | 19 | 1 | 2 | 180 | 204 | 385 | 1.14e-106 | 311 |
| MsG0080048567.01.T01 | ATMG01170-2 | 87.912 | 182 | 19 | 1 | 2 | 180 | 168 | 349 | 1.67e-106 | 309 |
| MsG0080048567.01.T02 | ATMG01170 | 90.476 | 147 | 14 | 0 | 1 | 147 | 239 | 385 | 1.35e-88 | 263 |
| MsG0080048567.01.T02 | AT2G07741 | 90.476 | 147 | 14 | 0 | 1 | 147 | 239 | 385 | 1.35e-88 | 263 |
| MsG0080048567.01.T02 | ATMG01170-2 | 90.476 | 147 | 14 | 0 | 1 | 147 | 203 | 349 | 3.30e-88 | 261 |
| MsG0080048567.01.T03 | ATMG01170-2 | 88.571 | 70 | 8 | 0 | 1 | 70 | 280 | 349 | 1.90e-37 | 127 |
| MsG0080048567.01.T03 | ATMG01170 | 88.571 | 70 | 8 | 0 | 1 | 70 | 316 | 385 | 2.05e-37 | 128 |
| MsG0080048567.01.T03 | AT2G07741 | 88.571 | 70 | 8 | 0 | 1 | 70 | 316 | 385 | 2.05e-37 | 128 |
Find 18 sgRNAs with CRISPR-Local
Find 36 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCATTAACAGGTCTGGAATT+AGG | 0.274974 | contig358end:+37657 | MsG0080048567.01.T01:CDS |
| TTAATAGTAATTGCATTAAC+AGG | 0.336241 | contig358end:+37645 | MsG0080048567.01.T01:CDS |
| AATGCAATTACTATTAATAT+AGG | 0.348864 | contig358end:-37639 | None:intergenic |
| TTATATTTCATAGGAGGGCT+TGG | 0.377245 | contig358end:+37615 | MsG0080048567.01.T01:CDS |
| ATAGTGGGATTTCAAAGAAA+TGG | 0.381988 | contig358end:+37393 | MsG0080048567.01.T01:CDS |
| CTTGTAACTGTAAAGCTATA+AGG | 0.404518 | contig358end:-37318 | None:intergenic |
| TAGTGGGATTTCAAAGAAAT+GGG | 0.421113 | contig358end:+37394 | MsG0080048567.01.T01:CDS |
| AGTAATTGCATTAACAGGTC+TGG | 0.434699 | contig358end:+37650 | MsG0080048567.01.T01:CDS |
| CACCTATTTGTTCATTTACC+AGG | 0.434773 | contig358end:-37215 | None:intergenic |
| ACGTTTATTTGCTAATATGA+TGG | 0.461716 | contig358end:+37527 | MsG0080048567.01.T01:CDS |
| TACCTGGTAAATGAACAAAT+AGG | 0.477446 | contig358end:+37213 | MsG0080048567.01.T01:CDS |
| TTATTTGCTAATATGATGGC+AGG | 0.479001 | contig358end:+37531 | MsG0080048567.01.T01:CDS |
| TTTATTGGTATAACAATAGT+GGG | 0.490587 | contig358end:+37378 | MsG0080048567.01.T01:CDS |
| AACGGCAGTGGTACTCCTGC+GGG | 0.559671 | contig358end:-37441 | None:intergenic |
| CTGGTAAATGAACAAATAGG+TGG | 0.591207 | contig358end:+37216 | MsG0080048567.01.T01:CDS |
| TTAAGTATATTATTACCCGC+AGG | 0.595683 | contig358end:+37426 | MsG0080048567.01.T01:CDS |
| TAACGGCAGTGGTACTCCTG+CGG | 0.628056 | contig358end:-37442 | None:intergenic |
| AACTATAAGAGATTAGCTCA+AGG | 0.628540 | contig358end:-37482 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATGCAATTACTATTAATAT+AGG | - | contig358end:37642-37661 | None:intergenic | 15.0% |
| !! | TATTGTTATACCAATAAAAA+TGG | - | contig358end:37376-37395 | None:intergenic | 15.0% |
| !!! | AATGATCTTTTATATTTCAT+AGG | + | contig358end:37606-37625 | MsG0080048567.01.T01:CDS | 15.0% |
| !! | TTAATAGTAATTGCATTAAC+AGG | + | contig358end:37645-37664 | MsG0080048567.01.T01:CDS | 20.0% |
| !! | TTTATTGGTATAACAATAGT+GGG | + | contig358end:37378-37397 | MsG0080048567.01.T01:CDS | 20.0% |
| !!! | ATCTTTTATATTTCATAGGA+GGG | + | contig358end:37610-37629 | MsG0080048567.01.T01:CDS | 20.0% |
| !!! | TTTTATTGGTATAACAATAG+TGG | + | contig358end:37377-37396 | MsG0080048567.01.T01:CDS | 20.0% |
| ! | TAAAAATGACAAAGATGCAA+GGG | - | contig358end:37263-37282 | None:intergenic | 25.0% |
| !! | ACGTTTATTTGCTAATATGA+TGG | + | contig358end:37527-37546 | MsG0080048567.01.T01:CDS | 25.0% |
| !! | AGTTTAGTCAAGATTTTAAG+TGG | + | contig358end:37558-37577 | MsG0080048567.01.T01:CDS | 25.0% |
| !! | GATCTTTTATATTTCATAGG+AGG | + | contig358end:37609-37628 | MsG0080048567.01.T01:CDS | 25.0% |
| !!! | AAGTCATTTTCTCATTACTT+TGG | + | contig358end:37338-37357 | MsG0080048567.01.T01:CDS | 25.0% |
| !!! | AGTCATTTTCTCATTACTTT+GGG | + | contig358end:37339-37358 | MsG0080048567.01.T01:CDS | 25.0% |
| !!! | CTCTCATTTTCCATTTTTAT+TGG | + | contig358end:37363-37382 | MsG0080048567.01.T01:CDS | 25.0% |
| !!! | TTGTTATTTTGTAATCTGCA+GGG | + | contig358end:37288-37307 | MsG0080048567.01.T01:CDS | 25.0% |
| CTTGTAACTGTAAAGCTATA+AGG | - | contig358end:37321-37340 | None:intergenic | 30.0% | |
| GTAAAAATGACAAAGATGCA+AGG | - | contig358end:37264-37283 | None:intergenic | 30.0% | |
| TACCTGGTAAATGAACAAAT+AGG | + | contig358end:37213-37232 | MsG0080048567.01.T01:CDS | 30.0% | |
| TAGTGGGATTTCAAAGAAAT+GGG | + | contig358end:37394-37413 | MsG0080048567.01.T01:CDS | 30.0% | |
| TTAAGTATATTATTACCCGC+AGG | + | contig358end:37426-37445 | MsG0080048567.01.T01:CDS | 30.0% | |
| ! | AACTATAAGAGATTAGCTCA+AGG | - | contig358end:37485-37504 | None:intergenic | 30.0% |
| ! | ATAGTGGGATTTCAAAGAAA+TGG | + | contig358end:37393-37412 | MsG0080048567.01.T01:CDS | 30.0% |
| ! | TTATTTGCTAATATGATGGC+AGG | + | contig358end:37531-37550 | MsG0080048567.01.T01:CDS | 30.0% |
| !! | AGTACTAAAAAAGGTGCTAA+CGG | - | contig358end:37462-37481 | None:intergenic | 30.0% |
| !!! | CTTGTTATTTTGTAATCTGC+AGG | + | contig358end:37287-37306 | MsG0080048567.01.T01:CDS | 30.0% |
| AGTAATTGCATTAACAGGTC+TGG | + | contig358end:37650-37669 | MsG0080048567.01.T01:CDS | 35.0% | |
| CACCTATTTGTTCATTTACC+AGG | - | contig358end:37218-37237 | None:intergenic | 35.0% | |
| CTGGTAAATGAACAAATAGG+TGG | + | contig358end:37216-37235 | MsG0080048567.01.T01:CDS | 35.0% | |
| TTATATTTCATAGGAGGGCT+TGG | + | contig358end:37615-37634 | MsG0080048567.01.T01:CDS | 35.0% | |
| !! | AGATTTTAAGTGGATTCGCA+TGG | + | contig358end:37568-37587 | MsG0080048567.01.T01:CDS | 35.0% |
| !! | AGCTCAAGGAGTACTAAAAA+AGG | - | contig358end:37471-37490 | None:intergenic | 35.0% |
| ! | AGTTTTCGCGCATTAAGCTT+AGG | + | contig358end:37501-37520 | MsG0080048567.01.T01:CDS | 40.0% |
| ! | GCATTAACAGGTCTGGAATT+AGG | + | contig358end:37657-37676 | MsG0080048567.01.T01:CDS | 40.0% |
| !! | AAAAAAGGTGCTAACGGCAG+TGG | - | contig358end:37456-37475 | None:intergenic | 45.0% |
| TAACGGCAGTGGTACTCCTG+CGG | - | contig358end:37445-37464 | None:intergenic | 55.0% | |
| AACGGCAGTGGTACTCCTGC+GGG | - | contig358end:37444-37463 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| contig358end | gene | 37213 | 37761 | 37213 | ID=MsG0080048567.01;Name=MsG0080048567.01 |
| contig358end | mRNA | 37213 | 37761 | 37213 | ID=MsG0080048567.01.T01;Parent=MsG0080048567.01;Name=MsG0080048567.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|182 |
| contig358end | exon | 37213 | 37761 | 37213 | ID=MsG0080048567.01.T01:exon:8081;Parent=MsG0080048567.01.T01 |
| contig358end | CDS | 37213 | 37761 | 37213 | ID=MsG0080048567.01.T01:cds;Parent=MsG0080048567.01.T01 |
| contig358end | mRNA | 37312 | 37761 | 37312 | ID=MsG0080048567.01.T02;Parent=MsG0080048567.01;Name=MsG0080048567.01.T02;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|149 |
| contig358end | exon | 37312 | 37761 | 37312 | ID=MsG0080048567.01.T02:exon:8082;Parent=MsG0080048567.01.T02 |
| contig358end | CDS | 37312 | 37761 | 37312 | ID=MsG0080048567.01.T02:cds;Parent=MsG0080048567.01.T02 |
| contig358end | mRNA | 37543 | 37761 | 37543 | ID=MsG0080048567.01.T03;Parent=MsG0080048567.01;Name=MsG0080048567.01.T03;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|72 |
| contig358end | exon | 37543 | 37761 | 37543 | ID=MsG0080048567.01.T03:exon:8083;Parent=MsG0080048567.01.T03 |
| contig358end | CDS | 37543 | 37761 | 37543 | ID=MsG0080048567.01.T03:cds;Parent=MsG0080048567.01.T03 |
| Gene Sequence |
| Protein sequence |
