AlfalfaGEDB | MsG0080048610.01

M. sativa cultivar ZhongmuNo.1 / MsG0080048610.01

KEGG: -
GO: GO:0003676
PFAM: PF13456

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0080048610.01.T01	XP_024634710.1	70.946	148	29	1	1	134	121	268	1.78E-70	226
MsG0080048610.01.T03	XP_024636349.1	83.562	73	12	0	3	75	99	171	2.57E-39	139

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0080048610.01.T01	A0A396IKN1	66.216	148	36	1	1	134	327	474	1.21e-61	207
MsG0080048610.01.T03	G7L640	80.822	73	14	0	3	75	55	127	4.94e-38	134

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI

Gene1	Gene2	Type
MsG0080048610.01	MsG0780040274.01	PPI
MsG0080048610.01	MsG0280010449.01	PPI
MsG0080048610.01	MsG0280009322.01	PPI
MsG0080048610.01	MsG0380013578.01	PPI
MsG0080048610.01	MsG0780041116.01	PPI
MsG0080048610.01	MsG0780041116.01	PPI
MsG0080048610.01	MsG0780041116.01	PPI
MsG0080048610.01	MsG0280009322.01	PPI
MsG0080048610.01	MsG0780041116.01	PPI
MsG0080048610.01	MsG0380013578.01	PPI
MsG0080048610.01	MsG0280010449.01	PPI
MsG0080048610.01	MsG0780040274.01	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0080048610.01.T01	MTR_6g046670	62.821	156	43	2	3	143	35	190	8.66e-65	196
MsG0080048610.01.T01	MTR_1g054905	64.925	134	33	1	1	120	11	144	1.44e-57	176
MsG0080048610.01.T01	MTR_7g013520	70.435	115	28	2	20	134	19	127	1.22e-55	172
MsG0080048610.01.T01	MTR_2g017550	47.651	149	57	3	2	129	12	160	1.25e-38	129
MsG0080048610.01.T01	MTR_4g029360	44.681	141	68	2	1	131	41	181	6.50e-35	121
MsG0080048610.01.T01	MTR_6g089560	55.670	97	43	0	44	140	83	179	6.98e-35	121
MsG0080048610.01.T01	MTR_8g099580	47.581	124	48	2	27	134	141	263	8.07e-32	115
MsG0080048610.01.T01	MTR_6g453270	40.523	153	75	2	1	137	208	360	2.46e-31	115
MsG0080048610.01.T01	MTR_6g045427	41.060	151	44	3	1	134	80	202	1.10e-30	110
MsG0080048610.01.T01	MTR_1g018300	60.000	85	28	1	52	136	38	116	2.46e-28	105
MsG0080048610.01.T01	MTR_8g031080	42.105	133	57	3	1	115	197	327	7.90e-28	106
MsG0080048610.01.T01	MTR_1g017840	58.537	82	34	0	53	134	54	135	8.10e-28	101
MsG0080048610.01.T01	MTR_7g078610	63.636	66	24	0	54	119	28	93	1.40e-27	98.6
MsG0080048610.01.T01	MTR_1g050488	39.130	138	68	2	1	134	30	155	2.12e-27	100
MsG0080048610.01.T01	MTR_5g049680	51.220	82	40	0	56	137	10	91	6.38e-26	94.7
MsG0080048610.01.T01	MTR_3g116210	36.923	130	80	1	8	137	30	157	2.49e-25	95.5
MsG0080048610.01.T01	MTR_5g036320	51.220	82	40	0	56	137	58	139	3.20e-25	94.0
MsG0080048610.01.T01	MTR_5g034450	57.534	73	31	0	62	134	34	106	2.15e-24	91.3
MsG0080048610.01.T01	MTR_1g022215	57.971	69	29	0	65	133	2	70	2.32e-24	90.1
MsG0080048610.01.T01	MTR_2g102247	42.857	98	52	2	1	96	53	148	2.86e-24	91.7
MsG0080048610.01.T01	MTR_4g046027	42.857	119	51	1	1	119	68	169	4.21e-24	92.0
MsG0080048610.01.T01	MTR_2g047100	57.333	75	17	1	60	134	1	60	9.47e-24	89.0
MsG0080048610.01.T01	MTR_1g034430	33.333	141	87	1	4	137	15	155	3.22e-23	90.1
MsG0080048610.01.T01	MTR_1g087480	42.202	109	63	0	1	109	112	220	3.99e-23	90.9
MsG0080048610.01.T01	MTR_1g099430	46.739	92	44	1	46	137	99	185	1.32e-22	89.0
MsG0080048610.01.T01	MTR_4g035890	36.111	108	69	0	4	111	41	148	2.55e-22	86.7
MsG0080048610.01.T01	MTR_5g083200	44.681	94	52	0	44	137	65	158	2.67e-22	88.2
MsG0080048610.01.T01	MTR_0002s1220	44.681	94	52	0	44	137	65	158	2.67e-22	88.2
MsG0080048610.01.T01	MTR_8g028785	32.026	153	64	2	1	137	190	318	2.69e-22	90.9
MsG0080048610.01.T01	MTR_4g094272	43.478	115	57	4	13	124	31	140	9.28e-22	85.1
MsG0080048610.01.T01	MTR_0015s0120	36.496	137	47	1	1	137	257	353	9.69e-22	89.4
MsG0080048610.01.T01	MTR_7g036825	43.478	92	52	0	1	92	109	200	1.27e-21	87.0
MsG0080048610.01.T01	MTR_3g111390	43.617	94	53	0	44	137	65	158	1.82e-21	85.9
MsG0080048610.01.T01	MTR_1g070820	43.617	94	53	0	44	137	65	158	1.82e-21	85.9
MsG0080048610.01.T01	MTR_2g027020	49.333	75	38	0	56	130	7	81	2.20e-21	85.5
MsG0080048610.01.T01	MTR_8g036780	50.649	77	38	0	56	132	13	89	2.60e-21	83.2
MsG0080048610.01.T01	MTR_6g016265	48.193	83	43	0	55	137	17	99	3.70e-21	83.2
MsG0080048610.01.T01	MTR_1g088650	48.837	86	44	0	46	131	55	140	3.88e-21	84.7
MsG0080048610.01.T01	MTR_8g031800	48.780	82	42	0	56	137	10	91	4.59e-21	82.4
MsG0080048610.01.T01	MTR_1g069475	37.500	136	72	1	15	137	89	224	6.55e-21	85.5
MsG0080048610.01.T01	MTR_1g081930	47.727	88	46	0	49	136	10	97	6.78e-21	82.4
MsG0080048610.01.T01	MTR_2g055490	58.065	62	26	0	82	143	12	73	7.59e-21	82.0
MsG0080048610.01.T01	MTR_1g074090	46.591	88	47	0	50	137	54	141	9.25e-21	82.8
MsG0080048610.01.T01	MTR_8g038340	50.000	80	40	0	56	135	168	247	1.65e-20	84.3
MsG0080048610.01.T01	MTR_5g037920	40.367	109	61	2	36	144	60	164	2.88e-20	83.6
MsG0080048610.01.T01	MTR_7g053400	30.657	137	75	1	1	137	119	235	3.81e-20	83.6
MsG0080048610.01.T01	MTR_8g079540	46.429	84	44	1	48	131	20	102	1.23e-19	79.0
MsG0080048610.01.T01	MTR_8g022750	33.333	144	47	2	1	144	161	255	1.34e-19	82.8
MsG0080048610.01.T01	MTR_3g102000	41.489	94	46	1	13	97	130	223	1.72e-18	78.6
MsG0080048610.01.T01	MTR_0093s0070	48.810	84	35	1	59	134	97	180	2.20e-18	78.2
MsG0080048610.01.T01	MTR_8g098925	47.727	88	32	1	47	134	49	122	2.58e-18	76.6
MsG0080048610.01.T01	MTR_5g085210	41.111	90	53	0	56	145	98	187	3.74e-18	77.8
MsG0080048610.01.T01	MTR_1g045340	43.373	83	47	0	55	137	62	144	7.09e-18	76.3
MsG0080048610.01.T01	MTR_2g059470	47.761	67	35	0	79	145	2	68	2.09e-17	72.4
MsG0080048610.01.T01	MTR_4g023580	31.452	124	67	2	1	124	61	166	1.65e-16	72.4
MsG0080048610.01.T01	MTR_8g069135	64.583	48	17	0	50	97	162	209	2.07e-16	74.3
MsG0080048610.01.T01	MTR_3g462490	44.444	90	47	1	31	117	52	141	2.11e-16	75.1
MsG0080048610.01.T01	MTR_8g039080	36.800	125	53	5	1	114	25	134	3.92e-16	72.4
MsG0080048610.01.T01	MTR_6g033560	61.818	55	21	0	1	55	341	395	7.31e-16	73.6
MsG0080048610.01.T01	MTR_6g016355	44.286	70	39	0	65	134	104	173	1.87e-15	69.7
MsG0080048610.01.T01	MTR_6g033225	39.785	93	52	1	46	134	35	127	3.18e-15	68.2
MsG0080048610.01.T01	MTR_1g074240	50.000	56	28	0	79	134	2	57	6.95e-15	65.5
MsG0080048610.01.T01	MTR_1964s0010	59.091	44	18	0	56	99	48	91	1.05e-14	65.9
MsG0080048610.01.T01	MTR_1g109000	41.975	81	47	0	56	136	5	85	1.19e-14	65.5
MsG0080048610.01.T01	MTR_4g008270	48.333	60	31	0	78	137	11	70	1.56e-14	65.1
MsG0080048610.01.T01	MTR_5g068115	50.909	55	27	0	56	110	13	67	1.69e-14	64.3
MsG0080048610.01.T01	MTR_3g085360	29.839	124	68	1	1	124	23	127	2.49e-14	65.5
MsG0080048610.01.T01	MTR_3g064060	46.154	52	28	0	46	97	6	57	2.59e-14	63.5
MsG0080048610.01.T01	MTR_2g054740	64.706	51	17	1	1	51	112	161	3.24e-14	66.6
MsG0080048610.01.T01	MTR_8g045330	40.260	77	42	1	56	132	76	148	5.72e-14	65.5
MsG0080048610.01.T01	MTR_4g094370	40.000	70	42	0	50	119	8	77	1.38e-13	62.4
MsG0080048610.01.T01	MTR_8g027950	42.466	73	42	0	1	73	98	170	2.83e-13	63.9
MsG0080048610.01.T01	MTR_7g063280	44.262	61	34	0	77	137	72	132	9.31e-13	62.4
MsG0080048610.01.T01	MTR_8g031330	36.620	71	45	0	2	72	93	163	1.37e-12	62.4
MsG0080048610.01.T01	MTR_3g010950	50.000	48	24	0	54	101	3	50	1.64e-12	60.8
MsG0080048610.01.T01	MTR_7g006380	48.214	56	29	0	54	109	43	98	3.40e-12	61.2
MsG0080048610.01.T01	MTR_8g059135	46.552	58	31	0	80	137	31	88	3.62e-12	60.1
MsG0080048610.01.T01	MTR_3g060800	54.545	44	20	0	54	97	18	61	3.64e-12	58.2
MsG0080048610.01.T01	MTR_1g115485	34.343	99	60	2	1	97	687	782	6.91e-12	62.4
MsG0080048610.01.T01	MTR_6g084160	35.556	90	48	2	4	90	126	208	1.10e-11	60.5
MsG0080048610.01.T01	MTR_5g075610	51.923	52	25	0	83	134	1	52	2.21e-11	57.4
MsG0080048610.01.T01	MTR_3g449470	29.452	146	87	5	1	134	68	209	2.57e-11	59.7
MsG0080048610.01.T01	MTR_5g042170	54.348	46	21	0	55	100	28	73	4.80e-11	57.0
MsG0080048610.01.T03	MTR_7g013520	80.822	73	14	0	3	75	55	127	1.25e-41	134
MsG0080048610.01.T03	MTR_6g046670	71.765	85	23	1	1	84	106	190	4.97e-38	125
MsG0080048610.01.T03	MTR_1g054905	83.051	59	10	0	3	61	86	144	6.89e-32	108
MsG0080048610.01.T03	MTR_6g089560	60.000	80	32	0	2	81	100	179	1.22e-29	105
MsG0080048610.01.T03	MTR_1g017840	64.384	73	26	0	3	75	63	135	1.26e-27	98.2
MsG0080048610.01.T03	MTR_8g099580	61.644	73	28	0	3	75	191	263	1.61e-27	101
MsG0080048610.01.T03	MTR_1g018300	63.158	76	22	1	2	77	47	116	1.62e-26	97.8
MsG0080048610.01.T03	MTR_5g034450	57.534	73	31	0	3	75	34	106	1.94e-25	91.3
MsG0080048610.01.T03	MTR_1g022215	57.971	69	29	0	6	74	2	70	2.38e-25	90.5
MsG0080048610.01.T03	MTR_5g049680	52.632	76	36	0	3	78	16	91	1.29e-24	89.0
MsG0080048610.01.T03	MTR_5g036320	52.632	76	36	0	3	78	64	139	1.68e-24	90.1
MsG0080048610.01.T03	MTR_7g078610	66.102	59	20	0	2	60	35	93	2.65e-24	87.8
MsG0080048610.01.T03	MTR_2g047100	57.333	75	17	1	1	75	1	60	4.82e-24	87.4
MsG0080048610.01.T03	MTR_0015s0120	53.947	76	35	0	3	78	278	353	1.15e-22	89.4
MsG0080048610.01.T03	MTR_1g034430	50.667	75	37	0	4	78	81	155	3.27e-22	85.1
MsG0080048610.01.T03	MTR_2g017550	56.944	72	31	0	3	74	93	164	3.79e-22	84.3
MsG0080048610.01.T03	MTR_6g453270	52.632	76	36	0	3	78	285	360	1.19e-21	87.0
MsG0080048610.01.T03	MTR_1g099430	48.684	76	39	0	3	78	110	185	1.58e-21	84.0
MsG0080048610.01.T03	MTR_2g055490	58.065	62	26	0	23	84	12	73	2.08e-21	81.3
MsG0080048610.01.T03	MTR_8g036780	52.113	71	34	0	3	73	19	89	5.85e-21	80.1
MsG0080048610.01.T03	MTR_1g088650	56.522	69	30	0	4	72	72	140	5.93e-21	82.0
MsG0080048610.01.T03	MTR_2g027020	52.174	69	33	0	3	71	13	81	1.33e-20	81.3
MsG0080048610.01.T03	MTR_8g031800	50.000	76	38	0	3	78	16	91	2.25e-20	78.6
MsG0080048610.01.T03	MTR_6g016265	50.000	76	38	0	3	78	24	99	3.57e-20	78.2
MsG0080048610.01.T03	MTR_8g038340	51.351	74	36	0	3	76	174	247	8.73e-20	80.1
MsG0080048610.01.T03	MTR_1g081930	52.000	75	36	0	3	77	23	97	1.31e-19	77.0
MsG0080048610.01.T03	MTR_3g116210	46.753	77	41	0	2	78	81	157	1.88e-19	78.2
MsG0080048610.01.T03	MTR_5g037920	46.429	84	44	1	3	86	83	165	2.22e-19	79.0
MsG0080048610.01.T03	MTR_1g050488	49.315	73	37	0	3	75	83	155	2.53e-19	77.0
MsG0080048610.01.T03	MTR_4g029360	55.714	70	31	0	3	72	112	181	3.00e-19	78.6
MsG0080048610.01.T03	MTR_1g074090	50.000	76	38	0	3	78	66	141	3.80e-19	76.3
MsG0080048610.01.T03	MTR_4g094272	58.065	62	25	1	4	65	80	140	3.93e-19	76.3
MsG0080048610.01.T03	MTR_1g069475	49.333	75	38	0	4	78	150	224	6.17e-19	77.8
MsG0080048610.01.T03	MTR_0093s0070	66.071	56	19	0	20	75	125	180	9.63e-19	76.6
MsG0080048610.01.T03	MTR_2g059470	47.761	67	35	0	20	86	2	68	3.77e-18	72.0
MsG0080048610.01.T03	MTR_5g083200	45.455	77	42	0	2	78	82	158	4.20e-18	74.7
MsG0080048610.01.T03	MTR_0002s1220	45.455	77	42	0	2	78	82	158	4.20e-18	74.7
MsG0080048610.01.T03	MTR_1g045340	45.455	77	42	0	2	78	68	144	9.87e-18	73.6
MsG0080048610.01.T03	MTR_8g031080	61.111	54	21	0	3	56	274	327	1.15e-17	75.9
MsG0080048610.01.T03	MTR_8g079540	50.746	67	33	0	6	72	36	102	1.99e-17	71.2
MsG0080048610.01.T03	MTR_3g111390	44.156	77	43	0	2	78	82	158	3.35e-17	72.4
MsG0080048610.01.T03	MTR_1g070820	44.156	77	43	0	2	78	82	158	3.35e-17	72.4
MsG0080048610.01.T03	MTR_6g016355	44.286	70	39	0	6	75	104	173	7.66e-17	71.2
MsG0080048610.01.T03	MTR_8g098925	64.815	54	19	0	22	75	69	122	1.78e-16	69.7
MsG0080048610.01.T03	MTR_5g085210	42.169	83	48	0	4	86	105	187	2.38e-16	70.9
MsG0080048610.01.T03	MTR_4g046027	54.237	59	27	0	2	60	111	169	6.59e-16	68.6
MsG0080048610.01.T03	MTR_4g008270	42.308	78	37	1	1	78	1	70	7.07e-16	66.6
MsG0080048610.01.T03	MTR_6g033225	44.000	75	42	0	1	75	53	127	1.43e-15	67.0
MsG0080048610.01.T03	MTR_8g022750	40.964	83	32	1	3	85	190	255	1.75e-15	69.3
MsG0080048610.01.T03	MTR_1g074240	50.000	56	28	0	20	75	2	57	1.96e-15	64.7
MsG0080048610.01.T03	MTR_3g462490	57.143	56	24	0	3	58	86	141	5.23e-15	68.6
MsG0080048610.01.T03	MTR_5g068115	55.102	49	22	0	3	51	19	67	1.27e-14	62.8
MsG0080048610.01.T03	MTR_4g094370	48.214	56	29	0	5	60	22	77	3.56e-14	61.6
MsG0080048610.01.T03	MTR_1g109000	44.444	72	40	0	6	77	14	85	5.85e-14	61.6
MsG0080048610.01.T03	MTR_7g063280	40.541	74	41	1	5	78	62	132	6.17e-14	63.5
MsG0080048610.01.T03	MTR_8g059135	46.552	58	31	0	21	78	31	88	1.68e-13	61.2
MsG0080048610.01.T03	MTR_8g069135	72.973	37	10	0	2	38	173	209	2.08e-13	63.9
MsG0080048610.01.T03	MTR_5g075610	51.923	52	25	0	24	75	1	52	1.27e-12	58.5
MsG0080048610.01.T03	MTR_4g035890	44.000	50	28	0	3	52	99	148	1.21e-11	57.0
MsG0080048610.01.T03	MTR_8g045330	38.889	72	40	1	2	73	81	148	1.41e-11	57.0
MsG0080048610.01.T03	MTR_1964s0010	60.526	38	15	0	3	40	54	91	1.50e-11	55.5
MsG0080048610.01.T03	MTR_7g006380	47.059	51	27	0	2	52	50	100	3.79e-11	56.2
MsG0080048610.01.T03	MTR_3g064060	58.333	36	15	0	3	38	22	57	7.00e-11	52.8
MsG0080048610.01.T03	MTR_8g028785	38.158	76	23	1	3	78	267	318	7.86e-11	56.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 50 sgRNAs with CRISPR-Local

Find 49 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TAACATTGTCCCAAACTTTA+AGG	0.074048	contig374end:+9818	None:intergenic
CGAAACGACTTGTATTAATT+TGG	0.162613	contig374end:+9374	None:intergenic
GTGGATGTGGGTGAAGCTTT+AGG	0.206587	contig374end:-9488	MsG0080048610.01.T01:CDS
GCATCCAATGGCCGTGAATA+TGG	0.220581	contig374end:-9695	MsG0080048610.01.T01:CDS
AACACCGCAACCTTAAAGTT+TGG	0.293942	contig374end:-9828	MsG0080048610.01.T01:CDS
GAGTGGGATGCAAGAATTTC+CGG	0.322226	contig374end:+9727	None:intergenic
TTTACAATGGTTGAGTGATA+TGG	0.341067	contig374end:-9453	MsG0080048610.01.T01:CDS
CTCGGAAGGTACGTTTGTTC+TGG	0.351033	contig374end:-9543	MsG0080048610.01.T01:CDS
CATCTTAACCGCACAGGTAT+TGG	0.365884	contig374end:-9581	MsG0080048610.01.T01:CDS
TGGATGTGGGTGAAGCTTTA+GGG	0.377571	contig374end:-9487	MsG0080048610.01.T01:CDS
CATCCAATGGCCGTGAATAT+GGG	0.390246	contig374end:-9694	MsG0080048610.01.T01:CDS
ACACCGCAACCTTAAAGTTT+GGG	0.405156	contig374end:-9827	MsG0080048610.01.T01:CDS
TTGTACTATTTCAGTGGATG+TGG	0.441573	contig374end:-9501	MsG0080048610.01.T01:CDS
CGGTGGTTGTCGAGCGTGCT+AGG	0.444430	contig374end:-9783	MsG0080048610.01.T01:CDS
ATTTATATCTACCTGACATA+GGG	0.465754	contig374end:+9630	None:intergenic
CATTTATATCTACCTGACAT+AGG	0.467496	contig374end:+9629	None:intergenic
GACCCCATATTCACGGCCAT+TGG	0.474510	contig374end:+9691	None:intergenic
GCCACAAGATTCTATTTCGC+TGG	0.477445	contig374end:+9657	None:intergenic
TGGCGTCTGTGTTCGCGACT+CGG	0.488724	contig374end:-9561	MsG0080048610.01.T01:CDS
TGTCCCAAACTTTAAGGTTG+CGG	0.498490	contig374end:+9824	None:intergenic
CTCGCCTTCTCAGCATCCAA+TGG	0.510545	contig374end:-9707	MsG0080048610.01.T01:intron
TCTTCTCATCTTAACCGCAC+AGG	0.521989	contig374end:-9587	MsG0080048610.01.T01:CDS
ACGGCCATTGGATGCTGAGA+AGG	0.535779	contig374end:+9703	None:intergenic
ATATCCTTGTACTATTTCAG+TGG	0.538487	contig374end:-9507	MsG0080048610.01.T01:CDS
GCGTGCTAGGAACATGGTTA+CGG	0.541598	contig374end:-9770	MsG0080048610.01.T01:CDS
CTAGGAACATGGTTACGGAT+TGG	0.549854	contig374end:-9765	MsG0080048610.01.T01:CDS
GGCTGCACGCTGCTTTACAA+TGG	0.555479	contig374end:-9466	MsG0080048610.01.T01:CDS
GTCTGTGTTCGCGACTCGGA+AGG	0.559975	contig374end:-9557	MsG0080048610.01.T01:CDS
TGAAACAGACTTTGAACGAC+GGG	0.564380	contig374end:-9411	MsG0080048610.01.T01:CDS
GCGGTTAAGATGAGAAGAAA+AGG	0.565238	contig374end:+9592	None:intergenic
AGGTGGAGACCCCATATTCA+CGG	0.571472	contig374end:+9684	None:intergenic
TCTATTTCGCTGGTGTGAGG+TGG	0.573438	contig374end:+9667	None:intergenic
CAACTGTTATGTGGAGCTTG+TGG	0.574627	contig374end:-9852	None:intergenic
ATCCAATGGCCGTGAATATG+GGG	0.589440	contig374end:-9693	MsG0080048610.01.T01:CDS
TGTACTATTTCAGTGGATGT+GGG	0.590537	contig374end:-9500	MsG0080048610.01.T01:CDS
GATTCTATTTCGCTGGTGTG+AGG	0.597473	contig374end:+9664	None:intergenic
TGTCGAGCGTGCTAGGAACA+TGG	0.599998	contig374end:-9776	MsG0080048610.01.T01:CDS
TTGGATGCTGAGAAGGCGAG+TGG	0.627773	contig374end:+9710	None:intergenic
TTTATATCTACCTGACATAG+GGG	0.637692	contig374end:+9631	None:intergenic
TTGGCAACTAGCGAATGCGC+CGG	0.644651	contig374end:-9746	MsG0080048610.01.T01:intron
ACATCCACTGAAATAGTACA+AGG	0.646072	contig374end:+9503	None:intergenic
TTGAAACAGACTTTGAACGA+CGG	0.647760	contig374end:-9412	MsG0080048610.01.T01:CDS
TGATATGGTGTTTGATAACG+TGG	0.654471	contig374end:-9438	MsG0080048610.01.T01:CDS
TTATATCTACCTGACATAGG+GGG	0.675819	contig374end:+9632	None:intergenic
ACCAGCGAAATAGAATCTTG+TGG	0.688611	contig374end:-9658	MsG0080048610.01.T01:CDS
TGGATGCTGAGAAGGCGAGT+GGG	0.696601	contig374end:+9711	None:intergenic
TGTTACAGAAACAAGTGCGG+TGG	0.701371	contig374end:-9800	MsG0080048610.01.T01:CDS
AGCGAAATAGAATCTTGTGG+CGG	0.701567	contig374end:-9655	MsG0080048610.01.T01:CDS
CACAGACGCCAATACCTGTG+CGG	0.753838	contig374end:+9573	None:intergenic
CAATGTTACAGAAACAAGTG+CGG	0.756313	contig374end:-9803	MsG0080048610.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	ATTTATATCTACCTGACATA+GGG	+	contig374end:9605-9624	None:intergenic	25.0%
	ATATCCTTGTACTATTTCAG+TGG	-	contig374end:9725-9744	MsG0080048610.01.T01:intron	30.0%
	CATTTATATCTACCTGACAT+AGG	+	contig374end:9606-9625	None:intergenic	30.0%
	TAACATTGTCCCAAACTTTA+AGG	+	contig374end:9417-9436	None:intergenic	30.0%
	TTTACAATGGTTGAGTGATA+TGG	-	contig374end:9779-9798	MsG0080048610.01.T01:CDS	30.0%
	TTTATATCTACCTGACATAG+GGG	+	contig374end:9604-9623	None:intergenic	30.0%
	ACATCCACTGAAATAGTACA+AGG	+	contig374end:9732-9751	None:intergenic	35.0%
	CAATGTTACAGAAACAAGTG+CGG	-	contig374end:9429-9448	MsG0080048610.01.T01:CDS	35.0%
	TGTACTATTTCAGTGGATGT+GGG	-	contig374end:9732-9751	MsG0080048610.01.T01:intron	35.0%
	TTATATCTACCTGACATAGG+GGG	+	contig374end:9603-9622	None:intergenic	35.0%
	TTGAAACAGACTTTGAACGA+CGG	-	contig374end:9820-9839	MsG0080048610.01.T01:CDS	35.0%
	TTGTACTATTTCAGTGGATG+TGG	-	contig374end:9731-9750	MsG0080048610.01.T01:intron	35.0%
!!	TGATATGGTGTTTGATAACG+TGG	-	contig374end:9794-9813	MsG0080048610.01.T01:CDS	35.0%
	AACACCGCAACCTTAAAGTT+TGG	-	contig374end:9404-9423	MsG0080048610.01.T01:CDS	40.0%
	ACACCGCAACCTTAAAGTTT+GGG	-	contig374end:9405-9424	MsG0080048610.01.T01:CDS	40.0%
	ACCAGCGAAATAGAATCTTG+TGG	-	contig374end:9574-9593	MsG0080048610.01.T01:CDS	40.0%
	AGCGAAATAGAATCTTGTGG+CGG	-	contig374end:9577-9596	MsG0080048610.01.T01:CDS	40.0%
	GCGGTTAAGATGAGAAGAAA+AGG	+	contig374end:9643-9662	None:intergenic	40.0%
	TGAAACAGACTTTGAACGAC+GGG	-	contig374end:9821-9840	MsG0080048610.01.T01:CDS	40.0%
!!	TGTCCCAAACTTTAAGGTTG+CGG	+	contig374end:9411-9430	None:intergenic	40.0%
	ATCCAATGGCCGTGAATATG+GGG	-	contig374end:9539-9558	MsG0080048610.01.T01:CDS	45.0%
	CATCCAATGGCCGTGAATAT+GGG	-	contig374end:9538-9557	MsG0080048610.01.T01:CDS	45.0%
	CATCTTAACCGCACAGGTAT+TGG	-	contig374end:9651-9670	MsG0080048610.01.T01:CDS	45.0%
	CTAGGAACATGGTTACGGAT+TGG	-	contig374end:9467-9486	MsG0080048610.01.T01:CDS	45.0%
	GAGTGGGATGCAAGAATTTC+CGG	+	contig374end:9508-9527	None:intergenic	45.0%
	GATTCTATTTCGCTGGTGTG+AGG	+	contig374end:9571-9590	None:intergenic	45.0%
	GCCACAAGATTCTATTTCGC+TGG	+	contig374end:9578-9597	None:intergenic	45.0%
	TCTTCTCATCTTAACCGCAC+AGG	-	contig374end:9645-9664	MsG0080048610.01.T01:CDS	45.0%
	TGGATGTGGGTGAAGCTTTA+GGG	-	contig374end:9745-9764	MsG0080048610.01.T01:intron	45.0%
	TGTTACAGAAACAAGTGCGG+TGG	-	contig374end:9432-9451	MsG0080048610.01.T01:CDS	45.0%
	AGGTGGAGACCCCATATTCA+CGG	+	contig374end:9551-9570	None:intergenic	50.0%
	GCATCCAATGGCCGTGAATA+TGG	-	contig374end:9537-9556	MsG0080048610.01.T01:CDS	50.0%
	GCGTGCTAGGAACATGGTTA+CGG	-	contig374end:9462-9481	MsG0080048610.01.T01:CDS	50.0%
	GTGGATGTGGGTGAAGCTTT+AGG	-	contig374end:9744-9763	MsG0080048610.01.T01:intron	50.0%
	TCTATTTCGCTGGTGTGAGG+TGG	+	contig374end:9568-9587	None:intergenic	50.0%
!	CTCGGAAGGTACGTTTGTTC+TGG	-	contig374end:9689-9708	MsG0080048610.01.T01:CDS	50.0%
	ACGGCCATTGGATGCTGAGA+AGG	+	contig374end:9532-9551	None:intergenic	55.0%
	CACAGACGCCAATACCTGTG+CGG	+	contig374end:9662-9681	None:intergenic	55.0%
	CTCGCCTTCTCAGCATCCAA+TGG	-	contig374end:9525-9544	MsG0080048610.01.T01:CDS	55.0%
	GACCCCATATTCACGGCCAT+TGG	+	contig374end:9544-9563	None:intergenic	55.0%
	TGGATGCTGAGAAGGCGAGT+GGG	+	contig374end:9524-9543	None:intergenic	55.0%
	TGTCGAGCGTGCTAGGAACA+TGG	-	contig374end:9456-9475	MsG0080048610.01.T01:CDS	55.0%
	TTGGATGCTGAGAAGGCGAG+TGG	+	contig374end:9525-9544	None:intergenic	55.0%
	TTGGCAACTAGCGAATGCGC+CGG	-	contig374end:9486-9505	MsG0080048610.01.T01:CDS	55.0%
!	GGCTGCACGCTGCTTTACAA+TGG	-	contig374end:9766-9785	MsG0080048610.01.T01:CDS	55.0%
	TGGCGTCTGTGTTCGCGACT+CGG	-	contig374end:9671-9690	MsG0080048610.01.T01:CDS	60.0%
!	GTCTGTGTTCGCGACTCGGA+AGG	-	contig374end:9675-9694	MsG0080048610.01.T01:CDS	60.0%
	CGGTGGTTGTCGAGCGTGCT+AGG	-	contig374end:9449-9468	MsG0080048610.01.T01:CDS	65.0%
	TTGTGGCGGCCCCCTATGTC+AGG	-	contig374end:9591-9610	MsG0080048610.01.T01:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
contig374end	gene	9388	9866	9388	ID=MsG0080048610.01;Name=MsG0080048610.01
contig374end	mRNA	9388	9866	9388	ID=MsG0080048610.01.T01;Parent=MsG0080048610.01;Name=MsG0080048610.01.T01;_AED=0.49;_eAED=0.50;_QI=0\|0\|0\|1\|0\|0\|2\|0\|145
contig374end	exon	9758	9866	9758	ID=MsG0080048610.01.T01:exon:37842;Parent=MsG0080048610.01.T01
contig374end	exon	9388	9716	9388	ID=MsG0080048610.01.T01:exon:37843;Parent=MsG0080048610.01.T01
contig374end	CDS	9758	9866	9758	ID=MsG0080048610.01.T01:cds;Parent=MsG0080048610.01.T01
contig374end	CDS	9388	9716	9388	ID=MsG0080048610.01.T01:cds;Parent=MsG0080048610.01.T01
contig374end	mRNA	9489	9779	9489	ID=MsG0080048610.01.T02;Parent=MsG0080048610.01;Name=MsG0080048610.01.T02;_AED=0.49;_eAED=0.49;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|96
contig374end	exon	9489	9779	9489	ID=MsG0080048610.01.T02:exon:37844;Parent=MsG0080048610.01.T02
contig374end	CDS	9489	9779	9489	ID=MsG0080048610.01.T02:cds;Parent=MsG0080048610.01.T02
contig374end	mRNA	9388	9648	9388	ID=MsG0080048610.01.T03;Parent=MsG0080048610.01;Name=MsG0080048610.01.T03;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|86
contig374end	exon	9388	9648	9388	ID=MsG0080048610.01.T03:exon:37845;Parent=MsG0080048610.01.T03
contig374end	CDS	9388	9648	9388	ID=MsG0080048610.01.T03:cds;Parent=MsG0080048610.01.T03
contig374end	mRNA	9489	9710	9489	ID=MsG0080048610.01.T04;Parent=MsG0080048610.01;Name=MsG0080048610.01.T04;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|73
contig374end	exon	9489	9710	9489	ID=MsG0080048610.01.T04:exon:37846;Parent=MsG0080048610.01.T04
contig374end	CDS	9489	9710	9489	ID=MsG0080048610.01.T04:cds;Parent=MsG0080048610.01.T04

Gene Sequence

>MsG0080048610.01.T02

ATGGTTACGGATTGGCAACTAGCGAATGCGCCGGAAATTCTTGCATCCCACTCGCCTTCTCAGCATCCAATGGCCGTGAATATGGGGTCTCCACCTCACACCAGCGAAATAGAATCTTGTGGCGGCCCCCTATGTCAGGTAGATATAAATGTAATATTGACGCAGCCTTTTCTTCTCATCTTAACCGCACAGGTATTGGCGTCTGTGTTCGCGACTCGGAAGGTACGTTTGTTCTGGCTAAGACTTTTACATATCCTTGTACTATTTCAGTGGATGTGGGTGAAGCTTTAG

>MsG0080048610.01.T04

ATGGCCGTGAATATGGGGTCTCCACCTCACACCAGCGAAATAGAATCTTGTGGCGGCCCCCTATGTCAGGTAGATATAAATGTAATATTGACGCAGCCTTTTCTTCTCATCTTAACCGCACAGGTATTGGCGTCTGTGTTCGCGACTCGGAAGGTACGTTTGTTCTGGCTAAGACTTTTACATATCCTTGTACTATTTCAGTGGATGTGGGTGAAGCTTTAG

>MsG0080048610.01.T01

ATGTGGAGCTTGTGGAAACACCGCAACCTTAAAGTTTGGGACAATGTTACAGAAACAAGTGCGGTGGTTGTCGAGCGTGCTAGGAACATGGTTACGGATTGGCAACTAGCATCCAATGGCCGTGAATATGGGGTCTCCACCTCACACCAGCGAAATAGAATCTTGTGGCGGCCCCCTATGTCAGGTAGATATAAATGTAATATTGACGCAGCCTTTTCTTCTCATCTTAACCGCACAGGTATTGGCGTCTGTGTTCGCGACTCGGAAGGTACGTTTGTTCTGGCTAAGACTTTTACATATCCTTGTACTATTTCAGTGGATGTGGGTGAAGCTTTAGGGCTGCACGCTGCTTTACAATGGTTGAGTGATATGGTGTTTGATAACGTGGACTTTGAAACAGACTTTGAACGACGGGAAGAAATGATACAACCAAATTAA

>MsG0080048610.01.T03

ATGTCAGGTAGATATAAATGTAATATTGACGCAGCCTTTTCTTCTCATCTTAACCGCACAGGTATTGGCGTCTGTGTTCGCGACTCGGAAGGTACGTTTGTTCTGGCTAAGACTTTTACATATCCTTGTACTATTTCAGTGGATGTGGGTGAAGCTTTAGGGCTGCACGCTGCTTTACAATGGTTGAGTGATATGGTGTTTGATAACGTGGACTTTGAAACAGACTTTGAACGACGGGAAGAAATGATACAACCAAATTAA

Protein sequence

>MsG0080048610.01.T02

MVTDWQLANAPEILASHSPSQHPMAVNMGSPPHTSEIESCGGPLCQVDINVILTQPFLLILTAQVLASVFATRKVRLFWLRLLHILVLFQWMWVKL*

>MsG0080048610.01.T04

MAVNMGSPPHTSEIESCGGPLCQVDINVILTQPFLLILTAQVLASVFATRKVRLFWLRLLHILVLFQWMWVKL*

>MsG0080048610.01.T01

MWSLWKHRNLKVWDNVTETSAVVVERARNMVTDWQLASNGREYGVSTSHQRNRILWRPPMSGRYKCNIDAAFSSHLNRTGIGVCVRDSEGTFVLAKTFTYPCTISVDVGEALGLHAALQWLSDMVFDNVDFETDFERREEMIQPN*

>MsG0080048610.01.T03

MSGRYKCNIDAAFSSHLNRTGIGVCVRDSEGTFVLAKTFTYPCTISVDVGEALGLHAALQWLSDMVFDNVDFETDFERREEMIQPN*