AlfalfaGEDB | MsG0780041116.01

M. sativa cultivar ZhongmuNo.1 / MsG0780041116.01

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780041116.01.T02	ABN05766.1	70.312	128	36	1	60	185	14	141	8.89E-50	167
MsG0780041116.01.T01	ABN05766.1	70.312	128	36	1	52	177	14	141	8.73E-50	167

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780041116.01.T01	A2Q1E9	70.312	128	36	1	52	177	14	141	4.17e-50	167

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI

Gene1	Gene2	Type
MsG0380016361.01	MsG0780041116.01	PPI
MsG0780041116.01	MsG0380013569.01	PPI
MsG0780041116.01	MsG0780038286.01	PPI
MsG0680035491.01	MsG0780041116.01	PPI
MsG0880043893.01	MsG0780041116.01	PPI
MsG0780039906.01	MsG0780041116.01	PPI
MsG0880045418.01	MsG0780041116.01	PPI
MsG0780041116.01	MsG0280008282.01	PPI
MsG0180001684.01	MsG0780041116.01	PPI
MsG0680032349.01	MsG0780041116.01	PPI
MsG0380016361.01	MsG0780041116.01	PPI
MsG0180001255.01	MsG0780041116.01	PPI
MsG0780041116.01	MsG0880044144.01	PPI
MsG0680032854.01	MsG0780041116.01	PPI
MsG0780041116.01	MsG0380013569.01	PPI
MsG0180001684.01	MsG0780041116.01	PPI
MsG0580025537.01	MsG0780041116.01	PPI
MsG0380014226.01	MsG0780041116.01	PPI
MsG0180001684.01	MsG0780041116.01	PPI
MsG0180001255.01	MsG0780041116.01	PPI
MsG0580030191.01	MsG0780041116.01	PPI
MsG0480022843.01	MsG0780041116.01	PPI
MsG0380014226.01	MsG0780041116.01	PPI
MsG0180001255.01	MsG0780041116.01	PPI
MsG0680032148.01	MsG0780041116.01	PPI
MsG0780041116.01	MsG0680034063.01	PPI
MsG0780041116.01	MsG0880044144.01	PPI
MsG0580030191.01	MsG0780041116.01	PPI
MsG0580025957.01	MsG0780041116.01	PPI
MsG0880043612.01	MsG0780041116.01	PPI
MsG0580030191.01	MsG0780041116.01	PPI
MsG0480022843.01	MsG0780041116.01	PPI
MsG0680035662.01	MsG0780041116.01	PPI
MsG0780041116.01	MsG0880043891.01	PPI
MsG0380016361.01	MsG0780041116.01	PPI
MsG0180001255.01	MsG0780041116.01	PPI
MsG0780041116.01	MsG0280008413.01	PPI
MsG0780041116.01	MsG0880043891.01	PPI
MsG0080048610.01	MsG0780041116.01	PPI
MsG0080048610.01	MsG0780041116.01	PPI
MsG0380016361.01	MsG0780041116.01	PPI
MsG0880043612.01	MsG0780041116.01	PPI
MsG0880043893.01	MsG0780041116.01	PPI
MsG0680035662.01	MsG0780041116.01	PPI
MsG0280006944.01	MsG0780041116.01	PPI
MsG0380016361.01	MsG0780041116.01	PPI
MsG0080048610.01	MsG0780041116.01	PPI
MsG0580025957.01	MsG0780041116.01	PPI
MsG0480021988.01	MsG0780041116.01	PPI
MsG0680035662.01	MsG0780041116.01	PPI
MsG0780041116.01	MsG0680034063.01	PPI
MsG0780041116.01	MsG0280009478.01	PPI
MsG0380014572.01	MsG0780041116.01	PPI
MsG0680032952.01	MsG0780041116.01	PPI
MsG0780039906.01	MsG0780041116.01	PPI
MsG0080048610.01	MsG0780041116.01	PPI
MsG0580030191.01	MsG0780041116.01	PPI
MsG0780041116.01	MsG0780038286.01	PPI
MsG0780041116.01	MsG0280009478.01	PPI
MsG0380014572.01	MsG0780041116.01	PPI
MsG0680032952.01	MsG0780041116.01	PPI
MsG0680032148.01	MsG0780041116.01	PPI
MsG0580025957.01	MsG0780041116.01	PPI
MsG0680035662.01	MsG0780041116.01	PPI
MsG0280009478.01	MsG0780041116.01	PPI
MsG0780041116.01	MsG0280009478.01	PPI
MsG0680032148.01	MsG0780041116.01	PPI
MsG0680032854.01	MsG0780041116.01	PPI
MsG0580025957.01	MsG0780041116.01	PPI
MsG0780041116.01	MsG0680032952.01	PPI
MsG0280009478.01	MsG0780041116.01	PPI
MsG0780041116.01	MsG0280008282.01	PPI
MsG0580025537.01	MsG0780041116.01	PPI
MsG0780041116.01	MsG0280009478.01	PPI
MsG0580025957.01	MsG0780041116.01	PPI
MsG0780041116.01	MsG0280008413.01	PPI
MsG0680033029.01	MsG0780041116.01	PPI
MsG0780038286.01	MsG0780041116.01	PPI
MsG0680032148.01	MsG0780041116.01	PPI
MsG0880045418.01	MsG0780041116.01	PPI
MsG0280006944.01	MsG0780041116.01	PPI
MsG0580025957.01	MsG0780041116.01	PPI
MsG0680032349.01	MsG0780041116.01	PPI
MsG0680033029.01	MsG0780041116.01	PPI
MsG0380016361.01	MsG0780041116.01	PPI
MsG0780038286.01	MsG0780041116.01	PPI
MsG0480021988.01	MsG0780041116.01	PPI
MsG0180001684.01	MsG0780041116.01	PPI
MsG0680035491.01	MsG0780041116.01	PPI
MsG0780041116.01	MsG0680032952.01	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0780041116.01.T01	MTR_2g028885	44.262	61	34	0	26	86	1	61	5.72e-11	57.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 21 sgRNAs with CRISPR-Local

Find 34 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TAAAGGAGTAGAATTGAAAT+AGG	0.338969	7:+86788108	None:intergenic
AAAATCAAGGCAATCGAGTC+AGG	0.379622	7:-86788314	MsG0780041116.01.T01:CDS
TTATCTTTAAGTTTGACTAT+AGG	0.383928	7:-86788021	MsG0780041116.01.T01:CDS
AAACACATTATTACGGGTCT+TGG	0.420243	7:-86788184	MsG0780041116.01.T01:intron
TCAAGTGTTAGACTTATTTG+AGG	0.432685	7:-86788080	MsG0780041116.01.T01:CDS
CTCATTATGTGGAGTAGAGT+TGG	0.469372	7:+86787976	None:intergenic
ATGGAGGCTAGGGTTGGATA+AGG	0.504751	7:+86787841	None:intergenic
TCTACTCCACATAATGAGTT+CGG	0.509220	7:-86787971	MsG0780041116.01.T01:CDS
GAAGTCTAACAACAACGCTC+TGG	0.524491	7:+86788141	None:intergenic
AGATATTATTTCTAGATGTA+CGG	0.531121	7:-86787948	MsG0780041116.01.T01:CDS
TCTAACACTTGAGCTTCTAA+AGG	0.532790	7:+86788091	None:intergenic
GTACACACCAACAAACTTTG+TGG	0.535840	7:-86788291	MsG0780041116.01.T01:CDS
CATATCACCACGGGAGGCGA+AGG	0.543331	7:+86788262	None:intergenic
TACACACCAACAAACTTTGT+GGG	0.560589	7:-86788290	MsG0780041116.01.T01:CDS
ATATCTCCGAACTCATTATG+TGG	0.561791	7:+86787965	None:intergenic
GTCTAACAACAACGCTCTGG+AGG	0.618220	7:+86788144	None:intergenic
TCCCGTGGTGATATGTAATG+TGG	0.622192	7:-86788254	MsG0780041116.01.T01:CDS
TCCACATTACATATCACCAC+GGG	0.636567	7:+86788253	None:intergenic
AAATCAAGGCAATCGAGTCA+GGG	0.639019	7:-86788313	MsG0780041116.01.T01:CDS
ACATTACATATCACCACGGG+AGG	0.671472	7:+86788256	None:intergenic
ATCCACATTACATATCACCA+CGG	0.685129	7:+86788252	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AATAAAAAAACACATTATTA+CGG	-	Chr7:86787918-86787937	MsG0780041116.01.T01:CDS	10.0%
!!	ATAAAAAAACACATTATTAC+GGG	-	Chr7:86787919-86787938	MsG0780041116.01.T01:CDS	15.0%
!!	ATGATACATCATGAAAAATA+TGG	+	Chr7:86788290-86788309	None:intergenic	20.0%
!!!	AGATATTATTTCTAGATGTA+CGG	-	Chr7:86788161-86788180	MsG0780041116.01.T01:CDS	20.0%
!!!	TTATCTTTAAGTTTGACTAT+AGG	-	Chr7:86788088-86788107	MsG0780041116.01.T01:CDS	20.0%
!!!	TTCATTTTATGAAAGAGTAA+CGG	+	Chr7:86788324-86788343	None:intergenic	20.0%
!	ATACATCATGAAAAATATGG+AGG	+	Chr7:86788287-86788306	None:intergenic	25.0%
!	TAAAGGAGTAGAATTGAAAT+AGG	+	Chr7:86788004-86788023	None:intergenic	25.0%
!!	TCATTTTATGAAAGAGTAAC+GGG	+	Chr7:86788323-86788342	None:intergenic	25.0%
	TCAAGTGTTAGACTTATTTG+AGG	-	Chr7:86788029-86788048	MsG0780041116.01.T01:CDS	30.0%
!	TACATTATTTTCTTGACTCC+TGG	+	Chr7:86788226-86788245	None:intergenic	30.0%
	AAACACATTATTACGGGTCT+TGG	-	Chr7:86787925-86787944	MsG0780041116.01.T01:CDS	35.0%
	ATATCTCCGAACTCATTATG+TGG	+	Chr7:86788147-86788166	None:intergenic	35.0%
	ATCCACATTACATATCACCA+CGG	+	Chr7:86787860-86787879	None:intergenic	35.0%
	CATGAAAAATATGGAGGCTA+GGG	+	Chr7:86788281-86788300	None:intergenic	35.0%
	TACACACCAACAAACTTTGT+GGG	-	Chr7:86787819-86787838	MsG0780041116.01.T01:CDS	35.0%
	TCATGAAAAATATGGAGGCT+AGG	+	Chr7:86788282-86788301	None:intergenic	35.0%
	TCTAACACTTGAGCTTCTAA+AGG	+	Chr7:86788021-86788040	None:intergenic	35.0%
	TCTACTCCACATAATGAGTT+CGG	-	Chr7:86788138-86788157	MsG0780041116.01.T01:CDS	35.0%
!	ATTTTATTGTGAGCTATGCC+AGG	-	Chr7:86788205-86788224	MsG0780041116.01.T01:intron	35.0%
!	GTTTTTCCCACAAAGTTTGT+TGG	+	Chr7:86787828-86787847	None:intergenic	35.0%
	AAAAATATGGAGGCTAGGGT+TGG	+	Chr7:86788277-86788296	None:intergenic	40.0%
	AAAATCAAGGCAATCGAGTC+AGG	-	Chr7:86787795-86787814	MsG0780041116.01.T01:CDS	40.0%
	AAATCAAGGCAATCGAGTCA+GGG	-	Chr7:86787796-86787815	MsG0780041116.01.T01:CDS	40.0%
	CTCATTATGTGGAGTAGAGT+TGG	+	Chr7:86788136-86788155	None:intergenic	40.0%
	GTACACACCAACAAACTTTG+TGG	-	Chr7:86787818-86787837	MsG0780041116.01.T01:CDS	40.0%
	TCCACATTACATATCACCAC+GGG	+	Chr7:86787859-86787878	None:intergenic	40.0%
	ACATTACATATCACCACGGG+AGG	+	Chr7:86787856-86787875	None:intergenic	45.0%
	GAAGTCTAACAACAACGCTC+TGG	+	Chr7:86787971-86787990	None:intergenic	45.0%
	TCCCGTGGTGATATGTAATG+TGG	-	Chr7:86787855-86787874	MsG0780041116.01.T01:CDS	45.0%
	ATGGAGGCTAGGGTTGGATA+AGG	+	Chr7:86788271-86788290	None:intergenic	50.0%
	GTCTAACAACAACGCTCTGG+AGG	+	Chr7:86787968-86787987	None:intergenic	50.0%
	CATATCACCACGGGAGGCGA+AGG	+	Chr7:86787850-86787869	None:intergenic	60.0%
	GGAAAAACCTTCGCCTCCCG+TGG	-	Chr7:86787840-86787859	MsG0780041116.01.T01:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
Chr7	gene	86787784	86788347	86787784	ID=MsG0780041116.01;Name=MsG0780041116.01
Chr7	mRNA	86787784	86788347	86787784	ID=MsG0780041116.01.T01;Parent=MsG0780041116.01;Name=MsG0780041116.01.T01;_AED=0.19;_eAED=0.19;_QI=0\|0\|0\|1\|0\|0\|2\|0\|177
Chr7	exon	86787784	86788190	86787784	ID=MsG0780041116.01.T01:exon:20405;Parent=MsG0780041116.01.T01
Chr7	exon	86788221	86788347	86788221	ID=MsG0780041116.01.T01:exon:20404;Parent=MsG0780041116.01.T01
Chr7	CDS	86788221	86788347	86788221	ID=MsG0780041116.01.T01:cds;Parent=MsG0780041116.01.T01
Chr7	CDS	86787784	86788190	86787784	ID=MsG0780041116.01.T01:cds;Parent=MsG0780041116.01.T01
Chr7	mRNA	86787784	86788341	86787784	ID=MsG0780041116.01.T02;Parent=MsG0780041116.01;Name=MsG0780041116.01.T02;_AED=0.18;_eAED=0.18;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|185
Chr7	exon	86787784	86788341	86787784	ID=MsG0780041116.01.T02:exon:20406;Parent=MsG0780041116.01.T02
Chr7	CDS	86787784	86788341	86787784	ID=MsG0780041116.01.T02:cds;Parent=MsG0780041116.01.T02

Gene Sequence

>MsG0780041116.01.T02

ATGCAAAAATCAAGGCAATCGAGTCAGGGTACACACCAACAAACTTTGTGGGAAAAACCTTCGCCTCCCGTGGTGATATGTAATGTGGATTGCGCACTCTTTAATAACAACACTATTACGAGTCTTGTAATAAAAAAACACATTATTACGGGTCTTGGCATCTGCTTTAGAGAGTCCTCCAGAGCGTTGTTGTTAGACTTCTCGAAGAGTTCCTATTTCAATTCTACTCCTTTAGAAGCTCAAGTGTTAGACTTATTTGAGGCTATAAACATTGATATTAATCGTCACGTGACATATGTTATCTTTAAGTTTGACTATAGGTTACTTGTTGATACTATTAACTCCAACTCTACTCCACATAATGAGTTCGGAGATATTATTTCTAGATGTACGGATCAATTGTCATCTCGCAATAATTTTATTGTGAGCTATGCCAGGAGTCAAGAAAATAATGTAGCTCATAACATAGTTAGAGCTTCCTTATCCAACCCTAGCCTCCATATTTTTCATGATGTATCATCTCATTTGTACCCGTTACTCTTTCATAAAATGAATTAG

>MsG0780041116.01.T01

ATGTGTATGCAAAAATCAAGGCAATCGAGTCAGGGTACACACCAACAAACTTTGTGGGAAAAACCTTCGCCTCCCGTGGTGATATGTAATGTGGATTGCGCACTCTTTAATAACAACACTATTACGAGTCTTGGCATCTGCTTTAGAGAGTCCTCCAGAGCGTTGTTGTTAGACTTCTCGAAGAGTTCCTATTTCAATTCTACTCCTTTAGAAGCTCAAGTGTTAGACTTATTTGAGGCTATAAACATTGATATTAATCGTCACGTGACATATGTTATCTTTAAGTTTGACTATAGGTTACTTGTTGATACTATTAACTCCAACTCTACTCCACATAATGAGTTCGGAGATATTATTTCTAGATGTACGGATCAATTGTCATCTCGCAATAATTTTATTGTGAGCTATGCCAGGAGTCAAGAAAATAATGTAGCTCATAACATAGTTAGAGCTTCCTTATCCAACCCTAGCCTCCATATTTTTCATGATGTATCATCTCATTTGTACCCGTTACTCTTTCATAAAATGAATTAG

Protein sequence

>MsG0780041116.01.T02

MQKSRQSSQGTHQQTLWEKPSPPVVICNVDCALFNNNTITSLVIKKHIITGLGICFRESSRALLLDFSKSSYFNSTPLEAQVLDLFEAINIDINRHVTYVIFKFDYRLLVDTINSNSTPHNEFGDIISRCTDQLSSRNNFIVSYARSQENNVAHNIVRASLSNPSLHIFHDVSSHLYPLLFHKMN*

>MsG0780041116.01.T01

MCMQKSRQSSQGTHQQTLWEKPSPPVVICNVDCALFNNNTITSLGICFRESSRALLLDFSKSSYFNSTPLEAQVLDLFEAINIDINRHVTYVIFKFDYRLLVDTINSNSTPHNEFGDIISRCTDQLSSRNNFIVSYARSQENNVAHNIVRASLSNPSLHIFHDVSSHLYPLLFHKMN*