Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048816.01.T01 | XP_013457616.1 | 97.414 | 116 | 3 | 0 | 1 | 116 | 1 | 116 | 1.83E-76 | 231 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048816.01.T01 | B7FMZ3 | 97.414 | 116 | 3 | 0 | 1 | 116 | 1 | 116 | 8.72e-77 | 231 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048816.01 | MsG0480018187.01 | 0.808525 | 2.967332e-50 | 1.700563e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0080048816.01.T01 | MTR_4g100975 | 97.414 | 116 | 3 | 0 | 1 | 116 | 1 | 116 | 2.21e-80 | 231 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 10 sgRNAs with CRISPR-Local
Find 12 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GAAGAAGTTGAATTAATTGA+AGG | 0.392329 | contig462end:+6016 | None:intergenic |
| ATGTGTGCTCAAGTTGTTGA+TGG | 0.431623 | contig462end:-6194 | MsG0080048816.01.T01:CDS |
| CAACAACTTGAGCACACATA+TGG | 0.485852 | contig462end:+6198 | None:intergenic |
| AGTTTCAACTGTACAAAGAA+TGG | 0.495744 | contig462end:-6083 | MsG0080048816.01.T01:CDS |
| TTAGCAAAACCTGCAGTAGC+TGG | 0.500324 | contig462end:-6232 | MsG0080048816.01.T01:CDS |
| GAGCGACAGTAGCATGTGTT+GGG | 0.520098 | contig462end:+6038 | None:intergenic |
| GGAGCGACAGTAGCATGTGT+TGG | 0.541010 | contig462end:+6037 | None:intergenic |
| TGTGTGCTCAAGTTGTTGAT+GGG | 0.543119 | contig462end:-6193 | MsG0080048816.01.T01:CDS |
| CTTTGTACAGTTGAAACTTG+AGG | 0.560559 | contig462end:+6088 | None:intergenic |
| AGACTTGCAAAATGATCAAG+AGG | 0.562188 | contig462end:-5981 | MsG0080048816.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TGAAAAGAAACAATTTTGAT+TGG | + | contig462end:6083-6102 | None:intergenic | 20.0% |
| ! | GAAGAAGTTGAATTAATTGA+AGG | + | contig462end:6207-6226 | None:intergenic | 25.0% |
| AGTTTCAACTGTACAAAGAA+TGG | - | contig462end:6137-6156 | MsG0080048816.01.T01:CDS | 30.0% | |
| AGACTTGCAAAATGATCAAG+AGG | - | contig462end:6239-6258 | MsG0080048816.01.T01:CDS | 35.0% | |
| CTTTGTACAGTTGAAACTTG+AGG | + | contig462end:6135-6154 | None:intergenic | 35.0% | |
| CAACAACTTGAGCACACATA+TGG | + | contig462end:6025-6044 | None:intergenic | 40.0% | |
| ! | ATGTGTGCTCAAGTTGTTGA+TGG | - | contig462end:6026-6045 | MsG0080048816.01.T01:CDS | 40.0% |
| ! | TGTGTGCTCAAGTTGTTGAT+GGG | - | contig462end:6027-6046 | MsG0080048816.01.T01:CDS | 40.0% |
| GTTGAAAAACCAGCTACTGC+AGG | + | contig462end:6000-6019 | None:intergenic | 45.0% | |
| TTAGCAAAACCTGCAGTAGC+TGG | - | contig462end:5988-6007 | MsG0080048816.01.T01:CDS | 45.0% | |
| GAGCGACAGTAGCATGTGTT+GGG | + | contig462end:6185-6204 | None:intergenic | 50.0% | |
| GGAGCGACAGTAGCATGTGT+TGG | + | contig462end:6186-6205 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| contig462end | gene | 5946 | 6296 | 5946 | ID=MsG0080048816.01;Name=MsG0080048816.01 |
| contig462end | mRNA | 5946 | 6296 | 5946 | ID=MsG0080048816.01.T01;Parent=MsG0080048816.01;Name=MsG0080048816.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|116 |
| contig462end | exon | 5946 | 6296 | 5946 | ID=MsG0080048816.01.T01:exon:5514;Parent=MsG0080048816.01.T01 |
| contig462end | CDS | 5946 | 6296 | 5946 | ID=MsG0080048816.01.T01:cds;Parent=MsG0080048816.01.T01 |
| Gene Sequence |
| Protein sequence |
