AlfalfaGEDB | MsG0480018187.01

M. sativa cultivar ZhongmuNo.1 / MsG0480018187.01

KEGG: -
GO: GO:0004672 GO:0005524 GO:0006468 GO:0007165
PFAM: PF00069 PF03822

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480018187.01.T01	XP_013454516.1	97.025	437	12	1	1	437	22	457	0	870

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480018187.01.T01	Q8W1D5	61.29	434	160	2	1	434	45	470	0	553

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480018187.01.T01	A0A072UFT8	97.025	437	12	1	1	437	22	457	0.0	870

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048816.01	MsG0480018187.01	0.808525	2.967332e-50	1.700563e-47
MsG0180001213.01	MsG0480018187.01	0.812047	5.159011e-51	3.245522e-48
MsG0180001795.01	MsG0480018187.01	0.827491	1.519717e-54	1.469400e-51
MsG0180003088.01	MsG0480018187.01	0.813168	2.934616e-51	1.902258e-48
MsG0180003128.01	MsG0480018187.01	0.800389	1.474514e-48	6.857317e-46
MsG0180005052.01	MsG0480018187.01	0.804800	1.814895e-49	9.445969e-47
MsG0280007915.01	MsG0480018187.01	0.816211	6.217204e-52	4.378268e-49
MsG0280011120.01	MsG0480018187.01	-0.804187	2.436094e-49	1.247977e-46
MsG0380013541.01	MsG0480018187.01	0.817048	4.035448e-52	2.907727e-49
MsG0380017226.01	MsG0480018187.01	0.816895	4.368611e-52	3.134431e-49

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480018187.01.T01	MTR_4g006970	97.025	437	12	1	1	437	22	457	0.0	870
MsG0480018187.01.T01	MTR_2g105010	77.136	433	89	3	1	428	16	443	0.0	664
MsG0480018187.01.T01	MTR_1g013700	60.000	430	150	2	1	429	15	423	7.26e-180	509
MsG0480018187.01.T01	MTR_7g072575	55.035	427	188	4	1	426	14	437	2.61e-167	478
MsG0480018187.01.T01	MTR_4g029020	55.220	431	182	5	1	429	14	435	8.21e-165	472
MsG0480018187.01.T01	MTR_5g075060	49.446	451	200	6	1	429	14	458	1.21e-150	436
MsG0480018187.01.T01	MTR_4g131060	51.860	430	181	7	1	425	20	428	2.16e-146	424
MsG0480018187.01.T01	MTR_3g466400	49.533	428	209	4	1	426	16	438	2.04e-142	415
MsG0480018187.01.T01	MTR_2g016340	49.655	435	204	6	1	428	14	440	2.44e-141	412
MsG0480018187.01.T01	MTR_2g049790	48.485	429	207	9	1	424	25	444	3.33e-138	404

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480018187.01.T01	AT5G25110	61.290	434	160	2	1	434	45	470	0.0	553
MsG0480018187.01.T01	AT5G10930	60.412	437	163	3	1	435	14	442	0.0	538
MsG0480018187.01.T01	AT2G25090	55.305	443	181	6	1	434	17	451	4.66e-167	478
MsG0480018187.01.T01	AT5G45820	54.545	429	177	3	1	428	14	425	8.08e-166	474
MsG0480018187.01.T01	AT5G58380	50.676	444	197	4	1	429	14	450	2.36e-154	446
MsG0480018187.01.T01	AT4G18700	51.039	433	198	6	1	423	28	456	1.92e-146	426
MsG0480018187.01.T01	AT4G30960	52.083	432	180	5	1	425	26	437	6.06e-146	423
MsG0480018187.01.T01	AT5G01810	52.570	428	182	6	1	427	14	421	3.51e-145	421
MsG0480018187.01.T01	AT5G01810	52.570	428	182	6	1	427	14	421	3.51e-145	421
MsG0480018187.01.T01	AT1G30270	48.598	428	208	7	1	424	33	452	5.06e-142	415

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Find 93 sgRNAs with CRISPR-Local

Find 112 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAGAAGTTATGGCAACAAA+GGG	0.532839	4:-1338582	MsG0480018187.01.T01:CDS
AAAGCTGATTTATGGTCTTG+TGG	0.435090	4:-1338234	MsG0480018187.01.T01:CDS
AAATGAGAGTGTAGCCATCA+AGG	0.545380	4:-1338706	MsG0480018187.01.T01:CDS
AACAATGTTGGAGAAACTTG+AGG	0.560233	4:-1337746	MsG0480018187.01.T01:CDS
AACAGGGAAGAAGGAGTTTG+TGG	0.439382	4:-1337695	MsG0480018187.01.T01:CDS
AACTCAACCACTGCCACCTC+TGG	0.437622	4:+1337601	None:intergenic
AACTTTGATATAAACATCGA+TGG	0.579328	4:+1337778	None:intergenic
AAGCTGATTTATGGTCTTGT+GGG	0.436886	4:-1338233	MsG0480018187.01.T01:CDS
AAGTTAAATTTGAAGATAAC+AGG	0.292267	4:-1337712	MsG0480018187.01.T01:CDS
AATATTGATTTCAATGAGAT+TGG	0.343630	4:-1337937	MsG0480018187.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAGAAGTTATGGCAACAAA+GGG	-	Chr4:1337663-1337682	MsG0480018187.01.T01:CDS	30.0%
ATAACATTCTCACCTTGAAA+AGG	+	Chr4:1338071-1338090	None:intergenic	30.0%
ATAATTTCATCGTTGTCACA+TGG	-	Chr4:1338404-1338423	MsG0480018187.01.T01:CDS	30.0%
ATTCTTCATGGAAAATGCAA+TGG	+	Chr4:1338258-1338277	None:intergenic	30.0%
CAAACAATATCTTTGAGTGA+AGG	+	Chr4:1338728-1338747	None:intergenic	30.0%
CTTCACTCAAAGATATTGTT+TGG	-	Chr4:1338726-1338745	MsG0480018187.01.T01:CDS	30.0%
TGATATTCCTGAATTGGTAA+TGG	-	Chr4:1338346-1338365	MsG0480018187.01.T01:CDS	30.0%
TGATGTTGATATTCCTGAAT+TGG	-	Chr4:1338340-1338359	MsG0480018187.01.T01:CDS	30.0%
TTCTTCATGGAAAATGCAAT+GGG	+	Chr4:1338257-1338276	None:intergenic	30.0%
TTGAAGAAGAAAGGTTATGA+TGG	-	Chr4:1337984-1338003	MsG0480018187.01.T01:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	1337477	1338790	1337477	ID=MsG0480018187.01;Name=MsG0480018187.01
Chr4	mRNA	1337477	1338790	1337477	ID=MsG0480018187.01.T01;Parent=MsG0480018187.01;Name=MsG0480018187.01.T01;_AED=0.49;_eAED=0.49;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|437
Chr4	exon	1337477	1338790	1337477	ID=MsG0480018187.01.T01:exon:921;Parent=MsG0480018187.01.T01
Chr4	CDS	1337477	1338790	1337477	ID=MsG0480018187.01.T01:cds;Parent=MsG0480018187.01.T01

Gene Sequence

>MsG0480018187.01.T01

ATGGGTAAAGTCTTGGGTCAAGGAAACTTTGCAAAGGTTTACCATTGTAAAAACCTTTCAGAAAATGAGAGTGTAGCCATCAAGGTGATAAAGAAAGAGAGGCTGAAAAAAGAGAGGCTGGTGAAGCAAATCAAGCGCGAAGTTTCAGTGATGCGTCTAGTTCGCCACCCTCATATCGTTGAACTCAAAGAAGTTATGGCAACAAAGGGAAAAATCTTTCTTGTAGTGGAATATGTAAAAGGAGGTGAACTCTTCAACAAGTTGGCGAAAGGAAAATTGAATGAAGATGTTGCTAGAAAGTACTTTCAGCAACTCATAAGTGCCGTAGATTTTTGCCATAGTCGTGGTGTCACACATCGCGATTTGAAACCAGAAAATCTACTCTTAGATGAAAACGAAGACCTCAAGGTCTCCGATTTTGGACTCTCGGCGTTGCCAGAACAACGCCGAGAGGATGGGATGCTTTTAACTCCGTGTGGAACCCCTGCTTACGTGGCACCTGAGGTTTTGAAGAAGAAAGGTTATGATGGTTCTAAAGCTGATTTATGGTCTTGTGGGGTAATTCTTTATGCTTTACTTTGTGGATATCTTCCTTTTCAAGGTGAGAATGTTATGAGGATTTATAGAAAGACTTTTAGAGGTGAATACGAGTTTCCTGAATGGATTTCTCCACAAGCTAAAATTTTGATTTCAAATCTTCTTGTTCCTGATCCTAAAAAGAGGTATTCAATTGTTGATATTATGAATGATCCTTGGTTTCGAGTTGGGTTTGTTAGACCCATTGCATTTTCCATGAAGAATTTGAGTATTGAAGACAAGGACAAAGATATTAATATTGATTTCAATGAGATTGGTGAAGATGATGATGTTGATATTCCTGAATTGGTAATGGTGAAACCGTCTCGTCCTTTTTATAATGCATTCGAGATAATTTCATCGTTGTCACATGGTTTTGATCTTAGAAGTTTGTTTGAGACGAGGAAGAGGTTGCCATCGATGTTTATATCAAAGTTTTCGGCTTCAACAATGTTGGAGAAACTTGAGGTGATGGCGAAGAAGTTAAATTTGAAGATAACAGGGAAGAAGGAGTTTGTGGTGAGAATGGAGGGCGCGAAGGAGGGGAGAAAGGGGAAAGTCGCGATGACAATGGAGGTATTTGAGGTGGCACCAGAGGTGGCAGTGGTTGAGTTTTCCAAGTGTGGTGGAGACACATTGGAGTATGTTAAGATGTGTGAGGAACAATTGAGACCTTCACTCAAAGATATTGTTTGGAGTTGGCAAGGTGACAATCATAATGATGATAATCCTCAATAG

Protein sequence

>MsG0480018187.01.T01

MGKVLGQGNFAKVYHCKNLSENESVAIKVIKKERLKKERLVKQIKREVSVMRLVRHPHIVELKEVMATKGKIFLVVEYVKGGELFNKLAKGKLNEDVARKYFQQLISAVDFCHSRGVTHRDLKPENLLLDENEDLKVSDFGLSALPEQRREDGMLLTPCGTPAYVAPEVLKKKGYDGSKADLWSCGVILYALLCGYLPFQGENVMRIYRKTFRGEYEFPEWISPQAKILISNLLVPDPKKRYSIVDIMNDPWFRVGFVRPIAFSMKNLSIEDKDKDINIDFNEIGEDDDVDIPELVMVKPSRPFYNAFEIISSLSHGFDLRSLFETRKRLPSMFISKFSASTMLEKLEVMAKKLNLKITGKKEFVVRMEGAKEGRKGKVAMTMEVFEVAPEVAVVEFSKCGGDTLEYVKMCEEQLRPSLKDIVWSWQGDNHNDDNPQ*