AlfalfaGEDB | MsG0080049112.01

M. sativa cultivar ZhongmuNo.1 / MsG0080049112.01

KEGG: -
GO: GO:0046983
PFAM: PF00010

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0080049112.01.T01	XP_013453691.1	96.757	185	4	1	1	183	1	185	6.43E-125	365

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0080049112.01.T01	Q9LSL1	71.93	57	14	1	129	183	151	207	1.32E-12	68.2

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0080049112.01.T01	A0A072UDL7	96.757	185	4	1	1	183	1	185	3.07e-125	365

TFs/TRs:

Gene ID	Type	Classification
MsG0080049112.01.T01	TF	bHLH

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080049112.01	MsG0580025575.01	0.865837	4.420511e-65	1.458757e-61

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0080049112.01.T01	MTR_5g030770	96.757	185	4	1	1	183	1	185	7.78e-129	365
MsG0080049112.01.T01	MTR_5g030770	96.757	185	4	1	1	183	1	185	8.86e-129	365
MsG0080049112.01.T01	MTR_7g076840	81.006	179	10	2	5	183	2	156	9.61e-88	258
MsG0080049112.01.T01	MTR_7g065690	48.649	148	36	6	30	177	26	133	1.58e-33	116
MsG0080049112.01.T01	MTR_3g111290	40.097	207	95	13	1	183	1	202	1.17e-16	76.6
MsG0080049112.01.T01	MTR_3g498695	68.182	44	11	1	140	183	287	327	7.40e-11	60.5
MsG0080049112.01.T01	MTR_3g498695	68.182	44	11	1	140	183	287	327	7.54e-11	60.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0080049112.01.T01	AT5G65640	71.930	57	14	1	129	183	151	207	6.63e-14	68.6
MsG0080049112.01.T01	AT5G65640	71.930	57	14	1	129	183	151	207	1.34e-13	68.2
MsG0080049112.01.T01	AT5G10570	36.000	200	78	9	11	183	4	180	3.34e-12	63.9
MsG0080049112.01.T01	AT3G26744	77.778	36	8	0	152	187	305	340	5.08e-11	60.8
MsG0080049112.01.T01	AT1G12860	77.778	36	8	0	152	187	265	300	6.14e-11	60.5

Find 37 sgRNAs with CRISPR-Local

Find 46 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGAATCACCTTGCTGATCTT+AGG	0.222682	contig77end:+17536	None:intergenic
AGGCTGTCCTTCAACCCTTT+TGG	0.223211	contig77end:+17634	None:intergenic
CATAGATTTGAATGTCCTTA+TGG	0.286074	contig77end:-17878	MsG0080049112.01.T01:CDS
TTTACCACAACAAGAAAGTA+TGG	0.308343	contig77end:-17786	MsG0080049112.01.T01:CDS
TCATCATACCCTTATCTTGA+TGG	0.320494	contig77end:-17845	MsG0080049112.01.T01:CDS
CTTTCTTGTTGTGGTAAAAC+TGG	0.323789	contig77end:+17791	None:intergenic
CAAGAAAGTATGGAAGAGTT+TGG	0.335442	contig77end:-17776	MsG0080049112.01.T01:CDS
GAGTCAAAGGTCCAACCATT+AGG	0.336189	contig77end:+17971	None:intergenic
ACAGCCTTCAAAGAATCTTA+TGG	0.358273	contig77end:-17618	MsG0080049112.01.T01:CDS
ATGGAAGAGTTTGGATTTGT+TGG	0.361028	contig77end:-17767	MsG0080049112.01.T01:CDS
CATTCAAATCTATGGTCTAA+TGG	0.372381	contig77end:+17887	None:intergenic
GAATCACCTTGCTGATCTTA+GGG	0.398992	contig77end:+17537	None:intergenic
CATAAGGACATTCAAATCTA+TGG	0.410090	contig77end:+17879	None:intergenic
AACGAGTTACTACTCCCTAA+TGG	0.410425	contig77end:-17986	MsG0080049112.01.T01:CDS
GCAAATGAAGGGTTTAAAGA+TGG	0.426120	contig77end:+17923	None:intergenic
GAAGAAAGCGTTTGAATGAC+AGG	0.437266	contig77end:-17583	MsG0080049112.01.T01:CDS
TCATCCATAAGATTCTTTGA+AGG	0.448575	contig77end:+17614	None:intergenic
AGTCAAAGGTCCAACCATTA+GGG	0.456761	contig77end:+17972	None:intergenic
AAAATGGAACTTTCTCAACT+TGG	0.469304	contig77end:-18070	MsG0080049112.01.T01:CDS
ATGACAGGCTTTCCATGCTT+AGG	0.479470	contig77end:-17568	MsG0080049112.01.T01:CDS
GCCAAATCCAAAAGGGTTGA+AGG	0.482278	contig77end:-17641	MsG0080049112.01.T01:CDS
ACTGAGAATCCATCAAGATA+AGG	0.498561	contig77end:+17836	None:intergenic
CTGAGAATCCATCAAGATAA+GGG	0.499837	contig77end:+17837	None:intergenic
TCTTCCATACTTTCTTGTTG+TGG	0.521400	contig77end:+17782	None:intergenic
ACATGGAATACTTTATCCAC+TGG	0.522465	contig77end:-18013	MsG0080049112.01.T01:CDS
AGAATCTTATGGATGAAAGA+AGG	0.538180	contig77end:-17607	MsG0080049112.01.T01:CDS
GATGATGCATCAGTTCCATA+AGG	0.540398	contig77end:+17863	None:intergenic
TTGTTGGAAGTGAGAACAAG+AGG	0.555475	contig77end:-17751	MsG0080049112.01.T01:CDS
AGTTACTACTCCCTAATGGT+TGG	0.572860	contig77end:-17982	MsG0080049112.01.T01:CDS
GTTGAGAATGAAGCAAATGA+AGG	0.578645	contig77end:+17911	None:intergenic
TAAAATCAGCTGCAAAGTTG+AGG	0.582223	contig77end:-17717	MsG0080049112.01.T01:CDS
TTGAGAATGAAGCAAATGAA+GGG	0.614806	contig77end:+17912	None:intergenic
TAGTAACTCGTTCAAACCAG+TGG	0.618072	contig77end:+17997	None:intergenic
GGTGCTGAATCATCAAACTC+AGG	0.624297	contig77end:+17812	None:intergenic
AGGGACAATTGACCTAAGCA+TGG	0.627542	contig77end:+17556	None:intergenic
TAGTTCCAAGAAAAGACACA+TGG	0.690258	contig77end:-18030	MsG0080049112.01.T01:CDS
AATTGTCCCTAAGATCAGCA+AGG	0.709185	contig77end:-17543	MsG0080049112.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	ATTTTCATCAAATGAGTCAA+AGG	+	contig77end:17638-17657	None:intergenic	25.0%
	AGAAAAAAGCCAAATCCAAA+AGG	-	contig77end:17944-17963	MsG0080049112.01.T01:CDS	30.0%
	AGAATCTTATGGATGAAAGA+AGG	-	contig77end:17986-18005	MsG0080049112.01.T01:CDS	30.0%
	CATAAGGACATTCAAATCTA+TGG	+	contig77end:17717-17736	None:intergenic	30.0%
	CATAGATTTGAATGTCCTTA+TGG	-	contig77end:17715-17734	MsG0080049112.01.T01:CDS	30.0%
	CATTCAAATCTATGGTCTAA+TGG	+	contig77end:17709-17728	None:intergenic	30.0%
	GAAAAAAGCCAAATCCAAAA+GGG	-	contig77end:17945-17964	MsG0080049112.01.T01:CDS	30.0%
	TCATCCATAAGATTCTTTGA+AGG	+	contig77end:17982-18001	None:intergenic	30.0%
	TTGAGAATGAAGCAAATGAA+GGG	+	contig77end:17684-17703	None:intergenic	30.0%
	TTTACCACAACAAGAAAGTA+TGG	-	contig77end:17807-17826	MsG0080049112.01.T01:CDS	30.0%
!	GAAACTCCAGTTTTTAACAT+GGG	-	contig77end:17910-17929	MsG0080049112.01.T01:CDS	30.0%
!	TGAAACTCCAGTTTTTAACA+TGG	-	contig77end:17909-17928	MsG0080049112.01.T01:CDS	30.0%
	ACAGCCTTCAAAGAATCTTA+TGG	-	contig77end:17975-17994	MsG0080049112.01.T01:CDS	35.0%
	ACATGGAATACTTTATCCAC+TGG	-	contig77end:17580-17599	MsG0080049112.01.T01:CDS	35.0%
	ACTGAGAATCCATCAAGATA+AGG	+	contig77end:17760-17779	None:intergenic	35.0%
	ATGGAAGAGTTTGGATTTGT+TGG	-	contig77end:17826-17845	MsG0080049112.01.T01:CDS	35.0%
	CACAAACCCATGTTAAAAAC+TGG	+	contig77end:17919-17938	None:intergenic	35.0%
	CTGAGAATCCATCAAGATAA+GGG	+	contig77end:17759-17778	None:intergenic	35.0%
	GCAAATGAAGGGTTTAAAGA+TGG	+	contig77end:17673-17692	None:intergenic	35.0%
	GTTGAGAATGAAGCAAATGA+AGG	+	contig77end:17685-17704	None:intergenic	35.0%
	TAAAATCAGCTGCAAAGTTG+AGG	-	contig77end:17876-17895	MsG0080049112.01.T01:CDS	35.0%
	TAGTTCCAAGAAAAGACACA+TGG	-	contig77end:17563-17582	MsG0080049112.01.T01:CDS	35.0%
	TCATCATACCCTTATCTTGA+TGG	-	contig77end:17748-17767	MsG0080049112.01.T01:CDS	35.0%
	TCTTCCATACTTTCTTGTTG+TGG	+	contig77end:17814-17833	None:intergenic	35.0%
!	CAAGAAAGTATGGAAGAGTT+TGG	-	contig77end:17817-17836	MsG0080049112.01.T01:CDS	35.0%
!	CTTTCTTGTTGTGGTAAAAC+TGG	+	contig77end:17805-17824	None:intergenic	35.0%
!	GTATTCCATGTGTCTTTTCT+TGG	+	contig77end:17571-17590	None:intergenic	35.0%
!	GTTTTTAACATGGGTTTGTG+TGG	-	contig77end:17919-17938	MsG0080049112.01.T01:CDS	35.0%
!!	TTCTCAACTTGGTTTTCTTG+AGG	-	contig77end:17534-17553	MsG0080049112.01.T01:CDS	35.0%
	AACGAGTTACTACTCCCTAA+TGG	-	contig77end:17607-17626	MsG0080049112.01.T01:CDS	40.0%
	AATTGTCCCTAAGATCAGCA+AGG	-	contig77end:18050-18069	MsG0080049112.01.T01:CDS	40.0%
	AGTCAAAGGTCCAACCATTA+GGG	+	contig77end:17624-17643	None:intergenic	40.0%
	AGTTACTACTCCCTAATGGT+TGG	-	contig77end:17611-17630	MsG0080049112.01.T01:CDS	40.0%
	GAATCACCTTGCTGATCTTA+GGG	+	contig77end:18059-18078	None:intergenic	40.0%
	GATGATGCATCAGTTCCATA+AGG	+	contig77end:17733-17752	None:intergenic	40.0%
	TAGTAACTCGTTCAAACCAG+TGG	+	contig77end:17599-17618	None:intergenic	40.0%
	TGAATCACCTTGCTGATCTT+AGG	+	contig77end:18060-18079	None:intergenic	40.0%
	TTGTTGGAAGTGAGAACAAG+AGG	-	contig77end:17842-17861	MsG0080049112.01.T01:CDS	40.0%
!	TCCTTCAACCCTTTTGGATT+TGG	+	contig77end:17956-17975	None:intergenic	40.0%
!!	GAAGAAAGCGTTTGAATGAC+AGG	-	contig77end:18010-18029	MsG0080049112.01.T01:CDS	40.0%
	AGGGACAATTGACCTAAGCA+TGG	+	contig77end:18040-18059	None:intergenic	45.0%
	ATGACAGGCTTTCCATGCTT+AGG	-	contig77end:18025-18044	MsG0080049112.01.T01:CDS	45.0%
	GAGTCAAAGGTCCAACCATT+AGG	+	contig77end:17625-17644	None:intergenic	45.0%
	GCCAAATCCAAAAGGGTTGA+AGG	-	contig77end:17952-17971	MsG0080049112.01.T01:CDS	45.0%
!	GGTGCTGAATCATCAAACTC+AGG	+	contig77end:17784-17803	None:intergenic	45.0%
!	AGGCTGTCCTTCAACCCTTT+TGG	+	contig77end:17962-17981	None:intergenic	50.0%

Chromosome	Type	Strat	End	Strand	Name
contig77end	gene	17526	18089	17526	ID=MsG0080049112.01;Name=MsG0080049112.01
contig77end	mRNA	17526	18089	17526	ID=MsG0080049112.01.T01;Parent=MsG0080049112.01;Name=MsG0080049112.01.T01;_AED=0.32;_eAED=0.32;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|187
contig77end	exon	17526	18089	17526	ID=MsG0080049112.01.T01:exon:29514;Parent=MsG0080049112.01.T01
contig77end	CDS	17526	18089	17526	ID=MsG0080049112.01.T01:cds;Parent=MsG0080049112.01.T01

Gene Sequence

>MsG0080049112.01.T01

ATGGAACTTTCTCAACTTGGTTTTCTTGAGGAGCTACTAGTTCCAAGAAAAGACACATGGAATACTTTATCCACTGGTTTGAACGAGTTACTACTCCCTAATGGTTGGACCTTTGACTCATTTGATGAAAATCTTCTCATCAATCCATCTTTAAACCCTTCATTTGCTTCATTCTCAACACCATTAGACCATAGATTTGAATGTCCTTATGGAACTGATGCATCATCATACCCTTATCTTGATGGATTCTCAGTTCCTGAGTTTGATGATTCAGCACCAGTTTTACCACAACAAGAAAGTATGGAAGAGTTTGGATTTGTTGGAAGTGAGAACAAGAGGTTTGAAGAGAGTAAAATCAGCTGCAAAGTTGAGGAACAAGTTAGTGAAACTCCAGTTTTTAACATGGGTTTGTGTGGTGAGAAAAAAGCCAAATCCAAAAGGGTTGAAGGACAGCCTTCAAAGAATCTTATGGATGAAAGAAGGAGAAGAAAGCGTTTGAATGACAGGCTTTCCATGCTTAGGTCAATTGTCCCTAAGATCAGCAAGGTGATTCAATATATATAA

Protein sequence

>MsG0080049112.01.T01

MELSQLGFLEELLVPRKDTWNTLSTGLNELLLPNGWTFDSFDENLLINPSLNPSFASFSTPLDHRFECPYGTDASSYPYLDGFSVPEFDDSAPVLPQQESMEEFGFVGSENKRFEESKISCKVEEQVSETPVFNMGLCGEKKAKSKRVEGQPSKNLMDERRRRKRLNDRLSMLRSIVPKISKVIQYI*