AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0080049164.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0080049164.01.T01 MTR_7g082860 77.273 44 10 0 103 146 895 938 6.56e-15 70.9
MsG0080049164.01.T01 MTR_7g082860 79.365 63 11 1 45 105 581 643 9.12e-15 70.5
MsG0080049164.01.T01 MTR_7g082860 100.000 31 0 0 1 31 298 328 2.07e-14 69.7
MsG0080049164.01.T01 MTR_7g082860 77.273 44 10 0 103 146 895 938 8.78e-15 70.5
MsG0080049164.01.T01 MTR_7g082860 79.365 63 11 1 45 105 581 643 9.77e-15 70.5
MsG0080049164.01.T01 MTR_7g082860 100.000 31 0 0 1 31 298 328 2.57e-14 69.3
MsG0080049164.01.T01 MTR_7g082860 77.273 44 10 0 103 146 895 938 9.27e-15 70.5
MsG0080049164.01.T01 MTR_7g082860 79.365 63 11 1 45 105 581 643 1.06e-14 70.5
MsG0080049164.01.T01 MTR_7g082860 100.000 31 0 0 1 31 298 328 2.60e-14 69.3
MsG0080049164.01.T01 MTR_7g082860 79.070 43 9 0 103 145 895 937 9.79e-15 70.5
MsG0080049164.01.T01 MTR_7g082860 79.365 63 11 1 45 105 581 643 1.39e-14 70.1
MsG0080049164.01.T01 MTR_7g082860 100.000 31 0 0 1 31 298 328 2.94e-14 69.3
MsG0080049164.01.T01 MTR_7g082860 79.070 43 9 0 103 145 895 937 9.89e-15 70.5
MsG0080049164.01.T01 MTR_7g082860 79.365 63 11 1 45 105 581 643 1.15e-14 70.5
MsG0080049164.01.T01 MTR_7g082860 100.000 31 0 0 1 31 298 328 2.83e-14 69.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 33 sgRNAs with CRISPR-Local

Find 235 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGTGAAGCGTGATAAGTTTA+AGG 0.175886 contig98end:-35992 MsG0080049164.01.T01:CDS
TTTCACTACCCTTCTTTCTT+TGG 0.280336 contig98end:+35172 None:intergenic
GCAACAATAACAAAATTCTT+TGG 0.326651 contig98end:-35090 MsG0080049164.01.T01:CDS
TAATTTGAATGCTCACTATA+CGG 0.331374 contig98end:+35935 None:intergenic
ATTCAAATTACGAAGAGCTT+AGG 0.380712 contig98end:-35922 MsG0080049164.01.T01:CDS
GCGTGATAAGTTTAAGGTTA+GGG 0.385563 contig98end:-35986 MsG0080049164.01.T01:CDS
GTGATACTCAATCTGATCAT+TGG 0.407977 contig98end:-35044 MsG0080049164.01.T01:CDS
AGCGTGATAAGTTTAAGGTT+AGG 0.431922 contig98end:-35987 MsG0080049164.01.T01:CDS
CAAAACAGGGGTTATTTACC+AGG 0.433593 contig98end:+32756 None:intergenic
GAGAGGGTACTCGAAGGTTG+TGG 0.448434 contig98end:-36043 MsG0080049164.01.T01:CDS
TTCAAATTACGAAGAGCTTA+GGG 0.459668 contig98end:-35921 MsG0080049164.01.T01:CDS
TTTCCCCTTCCAAAGAAAGA+AGG 0.470517 contig98end:-35181 MsG0080049164.01.T01:CDS
ACTACCCTTCTTTCTTTGGA+AGG 0.474740 contig98end:+35176 None:intergenic
CATGCGTTCCTGCCGTGAAA+AGG 0.476924 contig98end:-32800 MsG0080049164.01.T01:CDS
TACCCTTCTTTCTTTGGAAG+GGG 0.481215 contig98end:+35178 None:intergenic
TGAAGTTTCTTGTTCGAACC+TGG 0.497250 contig98end:-32774 MsG0080049164.01.T01:CDS
AAGGAGATTATGGTTGAGAG+AGG 0.501851 contig98end:-36060 None:intergenic
CTACCCTTCTTTCTTTGGAA+GGG 0.503850 contig98end:+35177 None:intergenic
AGTGCTCATCATGTACAAAC+AGG 0.508658 contig98end:-32865 MsG0080049164.01.T01:CDS
CTGATCATTGGATCGAAGCC+CGG 0.525546 contig98end:-35032 MsG0080049164.01.T01:intron
GGAAGCACATTACGAAAGAA+AGG 0.535279 contig98end:-32838 MsG0080049164.01.T01:CDS
AGAACCAAGTGGTTCAAAGC+AGG 0.538058 contig98end:-35112 MsG0080049164.01.T01:CDS
GTTGCCTGCTTTGAACCACT+TGG 0.540633 contig98end:+35108 None:intergenic
TTCCCCTTCCAAAGAAAGAA+GGG 0.547116 contig98end:-35180 MsG0080049164.01.T01:CDS
TGAGTATCACCAGGCATCAC+AGG 0.548445 contig98end:+35057 None:intergenic
AGAGGGTACTCGAAGGTTGT+GGG 0.550148 contig98end:-36042 MsG0080049164.01.T01:CDS
AGGAGATTATGGTTGAGAGA+GGG 0.552614 contig98end:-36059 None:intergenic
AAAGCAATGCCTGTGATGCC+TGG 0.569244 contig98end:-35066 MsG0080049164.01.T01:CDS
CATCATGTACAAACAGGCAA+TGG 0.598370 contig98end:-32859 MsG0080049164.01.T01:CDS
TGATCAGATTGAGTATCACC+AGG 0.643111 contig98end:+35048 None:intergenic
GGTTGAGAGAGGGTACTCGA+AGG 0.662563 contig98end:-36049 MsG0080049164.01.T01:CDS
ACTTCATAAGTCCATCCAGT+CGG 0.672960 contig98end:+36012 None:intergenic
AAAGCAAAATCAGAACCAAG+TGG 0.706189 contig98end:-35123 MsG0080049164.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGTTATTATTTATTATTTGA+TGG - contig98end:35323-35342 MsG0080049164.01.T01:intron 10.0%
!!! AAAATGATTTTTTTAACAAA+TGG - contig98end:34439-34458 MsG0080049164.01.T01:intron 10.0%
!!! AAATCTTCAAAAAAATTTTT+AGG + contig98end:34335-34354 None:intergenic 10.0%
!!! ATTTAGACAAAAAATAATTT+TGG - contig98end:34381-34400 MsG0080049164.01.T01:intron 10.0%
!!! TTGTTAAAAAAATCATTTTT+AGG + contig98end:34438-34457 None:intergenic 10.0%
!! AGTTTATGCAAATAATAAAT+GGG - contig98end:33881-33900 MsG0080049164.01.T01:intron 15.0%
!! TTGAATGATTGAAATAATTT+GGG - contig98end:35701-35720 MsG0080049164.01.T01:intron 15.0%
!! TTTGAATGATTGAAATAATT+TGG - contig98end:35700-35719 MsG0080049164.01.T01:intron 15.0%
!!! TTATTTTTTGTCTAAATACA+AGG + contig98end:34378-34397 None:intergenic 15.0%
!!! TTTAAAAAAAATCGATTTTG+AGG - contig98end:34241-34260 MsG0080049164.01.T01:intron 15.0%
!! CAGTTTATGCAAATAATAAA+TGG - contig98end:33880-33899 MsG0080049164.01.T01:intron 20.0%
!! GTTTATGCAAATAATAAATG+GGG - contig98end:33882-33901 MsG0080049164.01.T01:intron 20.0%
!!! AGATAAACAAATTTTCCAAT+TGG + contig98end:35850-35869 None:intergenic 20.0%
!!! ATAACTATAGTTTTGAAACA+AGG + contig98end:35309-35328 None:intergenic 20.0%
!!! ATAAGGTGTTATAAAGTAAA+GGG - contig98end:35823-35842 MsG0080049164.01.T01:intron 20.0%
!!! CATTAATTGCAGTTTTTATA+TGG - contig98end:35411-35430 MsG0080049164.01.T01:intron 20.0%
!!! GAAATAATTTGGGATTTTAA+AGG - contig98end:35711-35730 MsG0080049164.01.T01:intron 20.0%
!!! TTGAGGTTTGATTTTAAAAA+CGG + contig98end:33533-33552 None:intergenic 20.0%
!!! TTTTGTAAATGTTATACAAC+TGG - contig98end:34603-34622 MsG0080049164.01.T01:intron 20.0%
! AAACAGCACATCAAAAAAAT+TGG - contig98end:33559-33578 MsG0080049164.01.T01:intron 25.0%
! AAATAAACATTCAAAAGCCA+AGG + contig98end:35235-35254 None:intergenic 25.0%
! AATAAACATTCAAAAGCCAA+GGG + contig98end:35234-35253 None:intergenic 25.0%
! ATTCCGAAAAGAAAAAGAAA+GGG + contig98end:34799-34818 None:intergenic 25.0%
! ATTTCTAATTCTTGCAATCT+CGG - contig98end:33978-33997 MsG0080049164.01.T01:intron 25.0%
! CAAGAAACAACAATAATGAA+GGG + contig98end:35188-35207 None:intergenic 25.0%
! GCAACAATAACAAAATTCTT+TGG - contig98end:33732-33751 MsG0080049164.01.T01:intron 25.0%
! GTTAAAGTTTCAAATCATCA+AGG + contig98end:35026-35045 None:intergenic 25.0%
! TAATTTGAATGCTCACTATA+CGG + contig98end:32890-32909 None:intergenic 25.0%
! TATAAGTGCAAATTACTTCT+AGG + contig98end:34560-34579 None:intergenic 25.0%
! TATTCCGAAAAGAAAAAGAA+AGG + contig98end:34800-34819 None:intergenic 25.0%
! TTAATGCAGGAACTAAATAT+TGG - contig98end:35536-35555 MsG0080049164.01.T01:intron 25.0%
! TTCAAAATCTTCACTGATAT+AGG + contig98end:35686-35705 None:intergenic 25.0%
!! AACAAGCATGAGTTTTTAAA+GGG - contig98end:33048-33067 MsG0080049164.01.T01:intron 25.0%
!! ATATTTAGTTCCTGCATTAA+AGG + contig98end:35536-35555 None:intergenic 25.0%
!! ATTTGATGGTAAATAACACA+TGG - contig98end:35337-35356 MsG0080049164.01.T01:intron 25.0%
!! GATAAGGTGTTATAAAGTAA+AGG - contig98end:35822-35841 MsG0080049164.01.T01:intron 25.0%
!! GTTGGATGATTTTATAGTTT+TGG - contig98end:35647-35666 MsG0080049164.01.T01:intron 25.0%
!! TTATTTCCAGTCTTGAATTT+TGG - contig98end:34975-34994 MsG0080049164.01.T01:intron 25.0%
!! TTTGATGGTAAATAACACAT+GGG - contig98end:35338-35357 MsG0080049164.01.T01:intron 25.0%
!!! AGTTTTTAAAGGGTTTTAAG+CGG - contig98end:33058-33077 MsG0080049164.01.T01:intron 25.0%
!!! ATATATGAGAGTGTGTTTTA+AGG + contig98end:35155-35174 None:intergenic 25.0%
AAAGGGAAATATCAGTTCAA+GGG - contig98end:34003-34022 MsG0080049164.01.T01:intron 30.0%
AAGAAAAAGAAAGGGGAAAA+TGG + contig98end:34791-34810 None:intergenic 30.0%
AAGCAAGCATTACTATATTG+AGG - contig98end:33919-33938 MsG0080049164.01.T01:intron 30.0%
ACTATAAAATCATCCAACCA+AGG + contig98end:35645-35664 None:intergenic 30.0%
ACTCTAAATTGACTAACCTA+AGG - contig98end:33599-33618 MsG0080049164.01.T01:intron 30.0%
ACTCTATGCATAGAAATATC+AGG + contig98end:34088-34107 None:intergenic 30.0%
AGGGAGATGTATAATAAACT+TGG - contig98end:35616-35635 MsG0080049164.01.T01:intron 30.0%
ATTCAAATTACGAAGAGCTT+AGG - contig98end:32900-32919 MsG0080049164.01.T01:intron 30.0%
CAAATCATCAAGGCTAATAT+AGG + contig98end:35016-35035 None:intergenic 30.0%
CCAAGAAACAACAATAATGA+AGG + contig98end:35189-35208 None:intergenic 30.0%
GACAAACCGATTGAAAATAA+GGG - contig98end:35597-35616 MsG0080049164.01.T01:intron 30.0%
GTAAACATGTTGACAAAATG+AGG - contig98end:32993-33012 MsG0080049164.01.T01:intron 30.0%
TATAAAGTAAAGGGTCCAAT+TGG - contig98end:35832-35851 MsG0080049164.01.T01:intron 30.0%
TTCAAATTACGAAGAGCTTA+GGG - contig98end:32901-32920 MsG0080049164.01.T01:intron 30.0%
TTCCGAAAAGAAAAAGAAAG+GGG + contig98end:34798-34817 None:intergenic 30.0%
TTGTACATAGATAAAACAGC+AGG + contig98end:34921-34940 None:intergenic 30.0%
! ATGTTTCTTTTGCTTCTATG+TGG - contig98end:35468-35487 MsG0080049164.01.T01:intron 30.0%
! CAAATTACTTCTAGGAGTAA+GGG + contig98end:34552-34571 None:intergenic 30.0%
! CAACAAGCATGAGTTTTTAA+AGG - contig98end:33047-33066 MsG0080049164.01.T01:intron 30.0%
! CTTTAAAAACTCATGCTTGT+TGG + contig98end:33049-33068 None:intergenic 30.0%
! TGAACAGTTTTACCAGTATT+TGG - contig98end:34821-34840 MsG0080049164.01.T01:intron 30.0%
! TGTTTCTATTACACAGTTGT+TGG - contig98end:34872-34891 MsG0080049164.01.T01:intron 30.0%
! TTCCCCTTTCTTTTTCTTTT+CGG - contig98end:34793-34812 MsG0080049164.01.T01:intron 30.0%
!! CCTTCATTATTGTTGTTTCT+TGG - contig98end:35186-35205 MsG0080049164.01.T01:intron 30.0%
!! GTTTGATTTTAAAAACGGAC+TGG + contig98end:33528-33547 None:intergenic 30.0%
!! TATATTCGCCTTTTTAGTCA+GGG - contig98end:34576-34595 MsG0080049164.01.T01:intron 30.0%
!! TTATATTCGCCTTTTTAGTC+AGG - contig98end:34575-34594 MsG0080049164.01.T01:intron 30.0%
!!! AAAATGAGGAAGTATTTTGC+TGG - contig98end:33007-33026 MsG0080049164.01.T01:intron 30.0%
!!! ACATCTCCCTTATTTTCAAT+CGG + contig98end:35606-35625 None:intergenic 30.0%
AAAACAGACCCTGACTAAAA+AGG + contig98end:34587-34606 None:intergenic 35.0%
AAAAGCCAAGGGATGAAAAT+TGG + contig98end:35223-35242 None:intergenic 35.0%
AAAGCAAAATCAGAACCAAG+TGG - contig98end:33699-33718 MsG0080049164.01.T01:intron 35.0%
AACCACCCTAAATTTAGTGT+AGG + contig98end:34212-34231 None:intergenic 35.0%
AAGCTTCCAAAATTCAAGAC+TGG + contig98end:34984-35003 None:intergenic 35.0%
AAGGGAAATATCAGTTCAAG+GGG - contig98end:34004-34023 MsG0080049164.01.T01:intron 35.0%
ACCACCCTAAATTTAGTGTA+GGG + contig98end:34211-34230 None:intergenic 35.0%
ACTGCCATGATCTAAAGAAA+AGG + contig98end:35275-35294 None:intergenic 35.0%
AGTGAAGCGTGATAAGTTTA+AGG - contig98end:32830-32849 MsG0080049164.01.T01:CDS 35.0%
ATCAACCAAACCATGTTCTT+TGG + contig98end:34167-34186 None:intergenic 35.0%
ATGCAGGAACTAAATATTGG+TGG - contig98end:35539-35558 MsG0080049164.01.T01:intron 35.0%
CAAGCATTACTATATTGAGG+AGG - contig98end:33922-33941 MsG0080049164.01.T01:intron 35.0%
CAGGGACAGTATAAACTAAA+AGG + contig98end:35069-35088 None:intergenic 35.0%
GAAAGGGAAATATCAGTTCA+AGG - contig98end:34002-34021 MsG0080049164.01.T01:intron 35.0%
GACACATACTTCTTCTGTAA+AGG + contig98end:34047-34066 None:intergenic 35.0%
GGACAAACCGATTGAAAATA+AGG - contig98end:35596-35615 MsG0080049164.01.T01:intron 35.0%
GTGATACTCAATCTGATCAT+TGG - contig98end:33778-33797 MsG0080049164.01.T01:intron 35.0%
GTTCGTGCAAGATGTAAATA+TGG + contig98end:34667-34686 None:intergenic 35.0%
TACTATATTGAGGAGGCAAA+AGG - contig98end:33929-33948 MsG0080049164.01.T01:intron 35.0%
TACTATGCAACATGTTCAGA+AGG - contig98end:34734-34753 MsG0080049164.01.T01:intron 35.0%
TATAATAAACTTGGCGACCT+TGG - contig98end:35625-35644 MsG0080049164.01.T01:intron 35.0%
TCAACCAAACCATGTTCTTT+GGG + contig98end:34166-34185 None:intergenic 35.0%
TCTAAATACAAGGAGCTCAA+GGG + contig98end:34368-34387 None:intergenic 35.0%
TCTGAACATGTTGCATAGTA+TGG + contig98end:34734-34753 None:intergenic 35.0%
TGCAGATCATGAAATGTGTT+AGG - contig98end:34951-34970 MsG0080049164.01.T01:intron 35.0%
TTAGAGTCTTGACTTCGAAA+AGG + contig98end:33586-33605 None:intergenic 35.0%
TTCTTTAGATCATGGCAGTA+AGG - contig98end:35276-35295 MsG0080049164.01.T01:intron 35.0%
TTTCACTACCCTTCTTTCTT+TGG + contig98end:33653-33672 None:intergenic 35.0%
TTTCTCAATCAGCCAAATAC+TGG + contig98end:34836-34855 None:intergenic 35.0%
! AGCGTGATAAGTTTAAGGTT+AGG - contig98end:32835-32854 MsG0080049164.01.T01:CDS 35.0%
! ATCCTTAATTAGAAGCGTGT+AGG + contig98end:35782-35801 None:intergenic 35.0%
! GAAACTTCATCTCCTTTTCA+CGG + contig98end:36037-36056 None:intergenic 35.0%
! GCAAATTACTTCTAGGAGTA+AGG + contig98end:34553-34572 None:intergenic 35.0%
! GCGTGATAAGTTTAAGGTTA+GGG - contig98end:32836-32855 MsG0080049164.01.T01:CDS 35.0%
! TCCTCCTTTTCTTTAGATCA+TGG - contig98end:35268-35287 MsG0080049164.01.T01:intron 35.0%
! TTTAGTTTATACTGTCCCTG+TGG - contig98end:35069-35088 MsG0080049164.01.T01:CDS 35.0%
!! AGGAAAAAGAGAAAGTGTTC+AGG - contig98end:33273-33292 MsG0080049164.01.T01:intron 35.0%
!! ATGTGCTGTTTGTAATGTTG+AGG + contig98end:33550-33569 None:intergenic 35.0%
!! CAAGACTTGAGGTTTGATTT+TGG + contig98end:33492-33511 None:intergenic 35.0%
!! GAATGATGTTGGTGATGATA+CGG - contig98end:33112-33131 MsG0080049164.01.T01:intron 35.0%
!! GATTTGATAGTGCTTTCCTT+AGG + contig98end:33618-33637 None:intergenic 35.0%
!! TGCATTAAAGGCATTTCACA+CGG + contig98end:35524-35543 None:intergenic 35.0%
!! TTGAGGTTTGATTTTGGAAG+CGG + contig98end:33486-33505 None:intergenic 35.0%
!!! GAGGTTTTTGAAACCAAAGA+AGG - contig98end:32931-32950 MsG0080049164.01.T01:intron 35.0%
AAATTTCAGTTCCAACACCC+AGG - contig98end:33436-33455 MsG0080049164.01.T01:intron 40.0%
ACATAACACCCCAAAGAACA+TGG - contig98end:34154-34173 MsG0080049164.01.T01:intron 40.0%
ACCAAACACCAAAAACCACA+GGG + contig98end:35087-35106 None:intergenic 40.0%
ACTACCCTTCTTTCTTTGGA+AGG + contig98end:33649-33668 None:intergenic 40.0%
ACTCTGCACTACGAAAGATA+AGG - contig98end:35806-35825 MsG0080049164.01.T01:intron 40.0%
ACTTCATAAGTCCATCCAGT+CGG + contig98end:32813-32832 None:intergenic 40.0%
AGGAGACATCAATAAGGCAT+TGG - contig98end:33949-33968 MsG0080049164.01.T01:intron 40.0%
AGTGCTCATCATGTACAAAC+AGG - contig98end:35957-35976 MsG0080049164.01.T01:CDS 40.0%
ATTCTTGCAATCTCGGTGAA+AGG - contig98end:33985-34004 MsG0080049164.01.T01:intron 40.0%
CAACCAAACCATGTTCTTTG+GGG + contig98end:34165-34184 None:intergenic 40.0%
CACCCTAAATTTAGTGTAGG+GGG + contig98end:34209-34228 None:intergenic 40.0%
CACCTACACGCTTCTAATTA+AGG - contig98end:35777-35796 MsG0080049164.01.T01:intron 40.0%
CATCATGTACAAACAGGCAA+TGG - contig98end:35963-35982 MsG0080049164.01.T01:CDS 40.0%
CCACCCTAAATTTAGTGTAG+GGG + contig98end:34210-34229 None:intergenic 40.0%
CTCCCCCTACACTAAATTTA+GGG - contig98end:34204-34223 MsG0080049164.01.T01:intron 40.0%
GCCATGATCTAAAGAAAAGG+AGG + contig98end:35272-35291 None:intergenic 40.0%
GGCAAAAGGAGACATCAATA+AGG - contig98end:33943-33962 MsG0080049164.01.T01:intron 40.0%
GTAATCCAGAGCAAATTCAG+TGG - contig98end:35387-35406 MsG0080049164.01.T01:intron 40.0%
GTCTAAATACAAGGAGCTCA+AGG + contig98end:34369-34388 None:intergenic 40.0%
GTGAGTCTGTCATCTTGTAT+GGG - contig98end:33212-33231 MsG0080049164.01.T01:intron 40.0%
GTGTGAAATGCCTTTAATGC+AGG - contig98end:35523-35542 MsG0080049164.01.T01:intron 40.0%
TACAAGTTTCCAGCATCAGT+TGG + contig98end:33844-33863 None:intergenic 40.0%
TAGATCTGATCAGCATCTCT+GGG - contig98end:33341-33360 MsG0080049164.01.T01:intron 40.0%
TCATTGATGAGCAAGACTTG+AGG + contig98end:33503-33522 None:intergenic 40.0%
TCCTACATTGCTAAATGACG+AGG - contig98end:33253-33272 MsG0080049164.01.T01:intron 40.0%
TCCTCGTCATTTAGCAATGT+AGG + contig98end:33257-33276 None:intergenic 40.0%
TGAAGTTTCTTGTTCGAACC+TGG - contig98end:36048-36067 MsG0080049164.01.T01:CDS 40.0%
TGATCAGATTGAGTATCACC+AGG + contig98end:33777-33796 None:intergenic 40.0%
TGCTGATCAGATCTAACATC+TGG + contig98end:33336-33355 None:intergenic 40.0%
TGTGAGTCTGTCATCTTGTA+TGG - contig98end:33211-33230 MsG0080049164.01.T01:intron 40.0%
TTAGATCTGATCAGCATCTC+TGG - contig98end:33340-33359 MsG0080049164.01.T01:intron 40.0%
TTCCCCTTCCAAAGAAAGAA+GGG - contig98end:33642-33661 MsG0080049164.01.T01:intron 40.0%
TTCTTGCAATCTCGGTGAAA+GGG - contig98end:33986-34005 MsG0080049164.01.T01:intron 40.0%
TTGCTTCTATGTGGCAATGA+GGG - contig98end:35477-35496 MsG0080049164.01.T01:intron 40.0%
TTTCCCCTTCCAAAGAAAGA+AGG - contig98end:33641-33660 MsG0080049164.01.T01:intron 40.0%
TTTGCTTCTATGTGGCAATG+AGG - contig98end:35476-35495 MsG0080049164.01.T01:intron 40.0%
! AATGACCACTGAATTTGCTC+TGG + contig98end:35395-35414 None:intergenic 40.0%
! CTACCCTTCTTTCTTTGGAA+GGG + contig98end:33648-33667 None:intergenic 40.0%
! CTTAATTAGAAGCGTGTAGG+TGG + contig98end:35779-35798 None:intergenic 40.0%
! GAATTTGCTCTGGATTACAG+AGG + contig98end:35385-35404 None:intergenic 40.0%
! GGAAGCACATTACGAAAGAA+AGG - contig98end:35984-36003 MsG0080049164.01.T01:CDS 40.0%
! TACCCTTCTTTCTTTGGAAG+GGG + contig98end:33647-33666 None:intergenic 40.0%
! TATACTGTCCCTGTGGTTTT+TGG - contig98end:35076-35095 MsG0080049164.01.T01:CDS 40.0%
! TCAGATCTAACATCTGGCTT+CGG + contig98end:33330-33349 None:intergenic 40.0%
! TTGGGGTGTTATGTATCAGA+TGG + contig98end:34148-34167 None:intergenic 40.0%
! TTTGAGGTCGTGAATGATGT+TGG - contig98end:33101-33120 MsG0080049164.01.T01:intron 40.0%
!!! GAGTGTGTTTTAAGGAACTC+AGG + contig98end:35147-35166 None:intergenic 40.0%
AAAGAAGGTTGTTCCGACTG+TGG - contig98end:32946-32965 MsG0080049164.01.T01:intron 45.0%
AAATTTAGTGTAGGGGGAGG+AGG + contig98end:34203-34222 None:intergenic 45.0%
AAGAAGGTTGTTCCGACTGT+GGG - contig98end:32947-32966 MsG0080049164.01.T01:intron 45.0%
AAGCCCCGAAGTTGAAGAAT+CGG - contig98end:33816-33835 MsG0080049164.01.T01:intron 45.0%
AATTTAGTGTAGGGGGAGGA+GGG + contig98end:34202-34221 None:intergenic 45.0%
ACACCCCAAAGAACATGGTT+TGG - contig98end:34159-34178 MsG0080049164.01.T01:intron 45.0%
ACAGTCGGAACAACCTTCTT+TGG + contig98end:32947-32966 None:intergenic 45.0%
AGAACCAAGTGGTTCAAAGC+AGG - contig98end:33710-33729 MsG0080049164.01.T01:intron 45.0%
AGCCAAGGGATGAAAATTGG+TGG + contig98end:35220-35239 None:intergenic 45.0%
ATAAACTTGGCGACCTTGGT+TGG - contig98end:35629-35648 MsG0080049164.01.T01:intron 45.0%
ATACAAGGAGCTCAAGGGTA+AGG + contig98end:34363-34382 None:intergenic 45.0%
ATCATTCGATGAGCAAGACC+TGG + contig98end:33457-33476 None:intergenic 45.0%
CACCAAACACCAAAAACCAC+AGG + contig98end:35088-35107 None:intergenic 45.0%
CCCCTACACTAAATTTAGGG+TGG - contig98end:34207-34226 MsG0080049164.01.T01:intron 45.0%
CCTAAATTTAGTGTAGGGGG+AGG + contig98end:34206-34225 None:intergenic 45.0%
CCTCCCCCTACACTAAATTT+AGG - contig98end:34203-34222 MsG0080049164.01.T01:intron 45.0%
GCAAGATGTAAATATGGCGC+AGG + contig98end:34661-34680 None:intergenic 45.0%
GCCAAGGGATGAAAATTGGT+GGG + contig98end:35219-35238 None:intergenic 45.0%
TACAAGGAGCTCAAGGGTAA+GGG + contig98end:34362-34381 None:intergenic 45.0%
TCAACATCTAACCCCACAGT+CGG + contig98end:32962-32981 None:intergenic 45.0%
TCATTCGATGAGCAAGACCT+GGG + contig98end:33456-33475 None:intergenic 45.0%
TTCCTTTCTCATCCTCAAGC+AGG + contig98end:34696-34715 None:intergenic 45.0%
! AAGAGCTTAGGGAGTATGTG+AGG - contig98end:32912-32931 MsG0080049164.01.T01:intron 45.0%
! ACCCACCAATTTTCATCCCT+TGG - contig98end:35215-35234 MsG0080049164.01.T01:intron 45.0%
! CAGATCTAACATCTGGCTTC+GGG + contig98end:33329-33348 None:intergenic 45.0%
! CGACCTCAAAACCGATTTAC+CGG + contig98end:33091-33110 None:intergenic 45.0%
!! ACTCCGGTAAATCGGTTTTG+AGG - contig98end:33085-33104 MsG0080049164.01.T01:intron 45.0%
!! CTAGGAGTAAGGGTCTTGTT+TGG + contig98end:34542-34561 None:intergenic 45.0%
!! GTTTGATTTTGGAAGCGGAC+TGG + contig98end:33481-33500 None:intergenic 45.0%
!! TGGCACCGATTCTTCAACTT+CGG + contig98end:33824-33843 None:intergenic 45.0%
!!! TCCCTGTGGTTTTTGGTGTT+TGG - contig98end:35083-35102 MsG0080049164.01.T01:CDS 45.0%
!!! TGAGGAAGTATTTTGCTGGC+TGG - contig98end:33011-33030 MsG0080049164.01.T01:intron 45.0%
AAAGCAATGCCTGTGATGCC+TGG - contig98end:33756-33775 MsG0080049164.01.T01:intron 50.0%
AGAAGGTTGTTCCGACTGTG+GGG - contig98end:32948-32967 MsG0080049164.01.T01:intron 50.0%
AGCCTGCTTGAGGATGAGAA+AGG - contig98end:34691-34710 MsG0080049164.01.T01:intron 50.0%
AGTCACTACAGCACTCGAAG+AGG - contig98end:35568-35587 MsG0080049164.01.T01:intron 50.0%
CTGATCATTGGATCGAAGCC+CGG - contig98end:33790-33809 MsG0080049164.01.T01:intron 50.0%
GACAGACTCACAGCAACATC+TGG + contig98end:33203-33222 None:intergenic 50.0%
GAGAAAGAAGTGAAGCCGTC+TGG - contig98end:33152-33171 MsG0080049164.01.T01:intron 50.0%
TGAGTATCACCAGGCATCAC+AGG + contig98end:33768-33787 None:intergenic 50.0%
TTCAACTTCGGGGCTTTCAC+CGG + contig98end:33812-33831 None:intergenic 50.0%
! CTTCATCTCCTTTTCACGGC+AGG + contig98end:36033-36052 None:intergenic 50.0%
! GTTGCCTGCTTTGAACCACT+TGG + contig98end:33717-33736 None:intergenic 50.0%
!! AGAGGGTACTCGAAGGTTGT+GGG - contig98end:32780-32799 MsG0080049164.01.T01:CDS 50.0%
!! GAGTTGTTGTTTGCCCACGT+GGG - contig98end:33300-33319 MsG0080049164.01.T01:intron 50.0%
!! GCACCGATTCTTCAACTTCG+GGG + contig98end:33822-33841 None:intergenic 50.0%
!! GGCACCGATTCTTCAACTTC+GGG + contig98end:33823-33842 None:intergenic 50.0%
!! TGAGTTGTTGTTTGCCCACG+TGG - contig98end:33299-33318 MsG0080049164.01.T01:intron 50.0%
!! TTGCTGGCTGGTTGATGAGA+CGG - contig98end:33023-33042 MsG0080049164.01.T01:intron 50.0%
!!! GGTTTTAAGCGGTGTGACTC+CGG - contig98end:33069-33088 MsG0080049164.01.T01:intron 50.0%
ACGAACCTTCAGCCTGCTTG+AGG - contig98end:34681-34700 MsG0080049164.01.T01:intron 55.0%
AGAAGTGAAGCCGTCTGGTG+TGG - contig98end:33157-33176 MsG0080049164.01.T01:intron 55.0%
CATGCGTTCCTGCCGTGAAA+AGG - contig98end:36022-36041 MsG0080049164.01.T01:CDS 55.0%
CTCATCCTCAAGCAGGCTGA+AGG + contig98end:34689-34708 None:intergenic 55.0%
GAAGTGAAGCCGTCTGGTGT+GGG - contig98end:33158-33177 MsG0080049164.01.T01:intron 55.0%
GATGAGCAAGACCTGGGTGT+TGG + contig98end:33450-33469 None:intergenic 55.0%
GCGGTGTGACTCCGGTAAAT+CGG - contig98end:33077-33096 MsG0080049164.01.T01:intron 55.0%
TCAACTTCGGGGCTTTCACC+GGG + contig98end:33811-33830 None:intergenic 55.0%
TCTTTCTCGACGCTCTGCTC+TGG + contig98end:33140-33159 None:intergenic 55.0%
TGCGCAGCGTGTTGGAAAGA+TGG - contig98end:34631-34650 MsG0080049164.01.T01:intron 55.0%
TGGCATAATGCGCAGCGTGT+TGG - contig98end:34623-34642 MsG0080049164.01.T01:intron 55.0%
TTATCCCCTCCCACACCAGA+CGG + contig98end:33170-33189 None:intergenic 55.0%
! ACAGCACTCGAAGAGGCATG+CGG - contig98end:35575-35594 MsG0080049164.01.T01:intron 55.0%
! ACAGCAGGAAGCACGTCTTC+TGG + contig98end:34906-34925 None:intergenic 55.0%
!! GAATCGGTGCCAACTGATGC+TGG - contig98end:33832-33851 MsG0080049164.01.T01:intron 55.0%
!! GAGAGGGTACTCGAAGGTTG+TGG - contig98end:32779-32798 MsG0080049164.01.T01:CDS 55.0%
!! GGTTGAGAGAGGGTACTCGA+AGG - contig98end:32773-32792 MsG0080049164.01.T01:CDS 55.0%
!! GTTGTGGGTTTTGTGCCGAC+TGG - contig98end:32795-32814 MsG0080049164.01.T01:CDS 55.0%
!!! TGGGTTTTGTGCCGACTGGA+TGG - contig98end:32799-32818 MsG0080049164.01.T01:CDS 55.0%
TGAAGCCGTCTGGTGTGGGA+GGG - contig98end:33162-33181 MsG0080049164.01.T01:intron 60.0%
GAAGCCGTCTGGTGTGGGAG+GGG - contig98end:33163-33182 MsG0080049164.01.T01:intron 65.0%
GTGAAGCCGTCTGGTGTGGG+AGG - contig98end:33161-33180 MsG0080049164.01.T01:intron 65.0%
TGGCTTCGGGTGACCCACGT+GGG + contig98end:33316-33335 None:intergenic 65.0%
CTGGCTTCGGGTGACCCACG+TGG + contig98end:33317-33336 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
contig98end gene 32771 36073 32771 ID=MsG0080049164.01;Name=MsG0080049164.01
contig98end mRNA 32771 36073 32771 ID=MsG0080049164.01.T01;Parent=MsG0080049164.01;Name=MsG0080049164.01.T01;_AED=0.46;_eAED=0.46;_QI=0|0|0|1|0.5|0.66|3|0|146
contig98end exon 35920 36073 35920 ID=MsG0080049164.01.T01:exon:12523;Parent=MsG0080049164.01.T01
contig98end exon 35033 35204 35033 ID=MsG0080049164.01.T01:exon:12522;Parent=MsG0080049164.01.T01
contig98end exon 32771 32885 32771 ID=MsG0080049164.01.T01:exon:12521;Parent=MsG0080049164.01.T01
contig98end CDS 35920 36073 35920 ID=MsG0080049164.01.T01:cds;Parent=MsG0080049164.01.T01
contig98end CDS 35033 35204 35033 ID=MsG0080049164.01.T01:cds;Parent=MsG0080049164.01.T01
contig98end CDS 32771 32885 32771 ID=MsG0080049164.01.T01:cds;Parent=MsG0080049164.01.T01
Gene Sequence

>MsG0080049164.01.T01

ATGGTTGAGAGAGGGTACTCGAAGGTTGTGGGTTTTGTGCCGACTGGATGGACTTATGAAGTGAAGCGTGATAAGTTTAAGGTTAGGGAGAAGATTCGTGTAAGATTCATCTTGTGCCGTATAGTGAGCATTCAAATTACGAAGAGCTTAGGGAATTTCCCCTTCCAAAGAAAGAAGGGTAGTGAAAGCAAGTCAAACAAGAAAGTAAAAGTCAAAGCAAAATCAGAACCAAGTGGTTCAAAGCAGGCAACAATAACAAAATTCTTTGGCAAAGCAATGCCTGTGATGCCTGGTGATACTCAATCTGATCATTGGATCGAAGCCCGTGCTCATCATGTACAAACAGGCAATGGAAGCACATTACGAAAGAAAGGCATCATTGTTCATCTCATGCGTTCCTGCCGTGAAAAGGAGATGAAGTTTCTTGTTCGAACCTGGTAA
Protein sequence

>MsG0080049164.01.T01

MVERGYSKVVGFVPTGWTYEVKRDKFKVREKIRVRFILCRIVSIQITKSLGNFPFQRKKGSESKSNKKVKVKAKSEPSGSKQATITKFFGKAMPVMPGDTQSDHWIEARAHHVQTGNGSTLRKKGIIVHLMRSCREKEMKFLVRTW*