AlfalfaGEDB | MsG0180000074.01

M. sativa cultivar ZhongmuNo.1 / MsG0180000074.01

KEGG: K14488
GO: GO:0009733
PFAM: PF02519

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180000074.01.T01	XP_013466175.1	100	130	0	0	1	130	17	146	1.61E-92	273

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180000074.01.T01	O65695	50.746	67	33	0	18	84	40	106	1.49E-16	72.8

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180000074.01.T01	A0A072VF32	100.000	130	0	0	1	130	17	146	7.69e-93	273

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180000028.01	MsG0180000074.01	0.809687	1.673291e-50	9.889766e-48
MsG0180000074.01	MsG0180003905.01	0.803679	3.106635e-49	1.570826e-46
MsG0180000074.01	MsG0380016031.01	0.803344	3.646375e-49	1.827893e-46
MsG0180000074.01	MsG0780041560.01	0.802589	5.223282e-49	2.568448e-46

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180000074.01.T01	MTR_1g024085	100.000	130	0	0	1	130	17	146	1.95e-96	273
MsG0180000074.01.T01	MTR_4g072160	61.157	121	38	3	1	121	22	133	4.70e-44	141
MsG0180000074.01.T01	MTR_1g061570	43.077	130	59	4	1	127	1	118	1.35e-26	96.7
MsG0180000074.01.T01	MTR_7g101740	44.304	79	44	0	9	87	28	106	6.42e-19	76.6
MsG0180000074.01.T01	MTR_5g021820	55.738	61	27	0	18	78	37	97	7.53e-19	76.3
MsG0180000074.01.T01	MTR_3g117610	51.429	70	34	0	18	87	42	111	1.52e-18	75.5
MsG0180000074.01.T01	MTR_4g072190	42.857	84	48	0	1	84	16	99	1.78e-18	75.1
MsG0180000074.01.T01	MTR_8g076040	57.377	61	26	0	18	78	38	98	2.83e-18	74.7
MsG0180000074.01.T01	MTR_4g072910	49.351	77	33	3	3	77	20	92	1.10e-17	73.2
MsG0180000074.01.T01	MTR_4g072640	52.941	68	31	1	12	78	16	83	1.22e-17	72.8
MsG0180000074.01.T01	MTR_1g063950	50.000	64	32	0	18	81	45	108	1.35e-17	73.6
MsG0180000074.01.T01	MTR_4g072500	52.941	68	31	1	12	78	16	83	1.71e-17	72.4
MsG0180000074.01.T01	MTR_4g072940	51.562	64	30	1	20	82	56	119	1.77e-17	73.2
MsG0180000074.01.T01	MTR_4g072860	52.174	69	29	2	14	78	16	84	3.47e-17	71.6
MsG0180000074.01.T01	MTR_4g072880	51.429	70	30	2	13	78	15	84	4.08e-17	71.2
MsG0180000074.01.T01	MTR_4g072230	54.286	70	26	3	13	78	15	82	5.03e-17	70.9
MsG0180000074.01.T01	MTR_1g009150	45.833	72	39	0	20	91	70	141	5.10e-17	73.2
MsG0180000074.01.T01	MTR_8g461210	45.205	73	40	0	19	91	33	105	5.37e-17	71.6
MsG0180000074.01.T01	MTR_4g072530	52.941	68	31	1	12	78	16	83	8.42e-17	70.5
MsG0180000074.01.T01	MTR_3g109160	39.785	93	48	2	21	113	69	153	9.74e-17	72.4
MsG0180000074.01.T01	MTR_4g072820	50.725	69	33	1	10	77	9	77	1.44e-16	69.7
MsG0180000074.01.T01	MTR_4g072310	51.429	70	30	2	13	78	15	84	2.18e-16	69.3
MsG0180000074.01.T01	MTR_4g072570	46.667	75	39	1	5	78	9	83	2.56e-16	69.3
MsG0180000074.01.T01	MTR_4g072270	50.000	70	31	2	13	78	15	84	3.83e-16	68.9
MsG0180000074.01.T01	MTR_3g084250	45.455	77	39	2	2	77	8	82	4.64e-16	68.6
MsG0180000074.01.T01	MTR_1g094750	48.438	64	33	0	18	81	47	110	5.92e-16	68.9
MsG0180000074.01.T01	MTR_3g113310	52.542	59	28	0	20	78	41	99	6.00e-16	68.6
MsG0180000074.01.T01	MTR_4g072610	46.667	75	39	1	13	86	11	85	6.14e-16	68.2
MsG0180000074.01.T01	MTR_3g084230	52.542	59	27	1	20	77	24	82	7.08e-16	68.2
MsG0180000074.01.T01	MTR_4g072730	51.471	68	32	1	12	78	16	83	7.23e-16	68.2
MsG0180000074.01.T01	MTR_3g084150	49.275	69	34	1	12	79	16	84	7.63e-16	68.2
MsG0180000074.01.T01	MTR_4g124750	46.970	66	35	0	16	81	64	129	9.31e-16	69.3
MsG0180000074.01.T01	MTR_3g084180	49.275	69	34	1	12	79	16	84	1.02e-15	67.8
MsG0180000074.01.T01	MTR_3g084210	52.542	59	27	1	20	77	24	82	1.13e-15	67.8
MsG0180000074.01.T01	MTR_4g072220	45.679	81	32	2	10	78	4	84	1.18e-15	67.4
MsG0180000074.01.T01	MTR_3g084160	50.847	59	28	1	20	77	24	82	1.48e-15	67.4
MsG0180000074.01.T01	MTR_4g072480	55.000	60	26	1	20	78	24	83	1.54e-15	67.4
MsG0180000074.01.T01	MTR_4g072300	46.250	80	31	2	10	77	4	83	1.57e-15	67.4
MsG0180000074.01.T01	MTR_4g072250	52.857	70	28	3	13	78	15	83	1.59e-15	67.4
MsG0180000074.01.T01	MTR_4g072660	52.239	67	31	1	13	78	12	78	1.92e-15	67.0
MsG0180000074.01.T01	MTR_7g405740	44.776	67	37	0	15	81	61	127	2.13e-15	68.6
MsG0180000074.01.T01	MTR_4g072680	43.590	78	43	1	5	81	9	86	2.30e-15	66.6
MsG0180000074.01.T01	MTR_2g043960	47.761	67	35	0	18	84	73	139	2.32e-15	68.6
MsG0180000074.01.T01	MTR_4g005320	46.154	65	35	0	21	85	70	134	2.63e-15	68.6
MsG0180000074.01.T01	MTR_3g092220	42.667	75	41	1	19	91	31	105	2.81e-15	67.0
MsG0180000074.01.T01	MTR_4g072240	44.444	81	33	2	10	78	4	84	3.24e-15	66.2
MsG0180000074.01.T01	MTR_4g072460	43.590	78	43	1	5	81	9	86	3.47e-15	66.2
MsG0180000074.01.T01	MTR_5g008370	54.098	61	27	1	19	78	23	83	3.76e-15	67.4
MsG0180000074.01.T01	MTR_8g461410	47.826	69	35	1	12	79	16	84	3.99e-15	66.2
MsG0180000074.01.T01	MTR_8g461420	50.847	59	28	1	20	77	24	82	4.54e-15	66.2
MsG0180000074.01.T01	MTR_4g072980	52.174	69	29	3	12	77	18	85	4.79e-15	66.2
MsG0180000074.01.T01	MTR_3g117640	63.934	61	18	3	20	77	33	92	5.10e-15	66.2
MsG0180000074.01.T01	MTR_4g072330	47.761	67	34	1	12	77	14	80	5.66e-15	65.9
MsG0180000074.01.T01	MTR_4g072870	52.459	61	28	1	20	79	25	85	6.97e-15	65.5
MsG0180000074.01.T01	MTR_4g072620	53.333	60	27	1	20	78	24	83	7.22e-15	65.5
MsG0180000074.01.T01	MTR_3g084190	49.153	59	29	1	20	77	24	82	1.01e-14	65.1
MsG0180000074.01.T01	MTR_3g084240	49.254	67	33	1	12	77	16	82	1.17e-14	65.1
MsG0180000074.01.T01	MTR_3g110032	47.761	67	34	1	12	77	17	83	1.20e-14	65.1
MsG0180000074.01.T01	MTR_3g098970	38.272	81	50	0	1	81	33	113	1.25e-14	66.2
MsG0180000074.01.T01	MTR_2g066830	45.833	72	38	1	11	81	47	118	1.32e-14	66.2
MsG0180000074.01.T01	MTR_3g110045	50.847	59	28	1	20	77	83	141	1.36e-14	66.2
MsG0180000074.01.T01	MTR_4g072850	51.667	60	28	1	20	78	26	85	1.36e-14	64.7
MsG0180000074.01.T01	MTR_4g072540	52.542	59	27	1	20	77	20	78	1.56e-14	64.7
MsG0180000074.01.T01	MTR_8g096470	44.615	65	36	0	22	86	47	111	1.63e-14	65.1
MsG0180000074.01.T01	MTR_4g072580	47.059	68	35	1	20	86	19	86	1.68e-14	64.3
MsG0180000074.01.T01	MTR_7g118010	47.887	71	35	1	21	89	23	93	1.71e-14	65.1
MsG0180000074.01.T01	MTR_3g084170	49.254	67	33	1	12	77	16	82	1.75e-14	64.7
MsG0180000074.01.T01	MTR_4g072830	44.872	78	37	2	1	77	8	80	2.35e-14	64.3
MsG0180000074.01.T01	MTR_4g072650	45.588	68	36	1	20	86	18	85	2.48e-14	63.9
MsG0180000074.01.T01	MTR_4g072260	43.750	80	33	2	10	77	4	83	3.15e-14	63.9
MsG0180000074.01.T01	MTR_3g110042	49.254	67	33	1	12	77	16	82	3.23e-14	63.9
MsG0180000074.01.T01	MTR_8g026730	47.826	69	34	1	21	87	27	95	4.12e-14	63.9
MsG0180000074.01.T01	MTR_4g072670	54.237	59	26	1	20	77	20	78	4.16e-14	63.5
MsG0180000074.01.T01	MTR_3g117630	47.761	67	34	1	12	77	16	82	4.77e-14	63.5
MsG0180000074.01.T01	MTR_4g025830	43.210	81	42	2	14	91	72	151	4.95e-14	65.1
MsG0180000074.01.T01	MTR_4g072600	47.059	68	35	1	20	86	19	86	5.12e-14	63.2
MsG0180000074.01.T01	MTR_4g072890	43.750	80	33	2	10	77	4	83	5.72e-14	63.2
MsG0180000074.01.T01	MTR_4g072590	49.180	61	30	1	20	79	19	79	6.31e-14	62.8
MsG0180000074.01.T01	MTR_4g072740	50.820	61	29	1	20	79	19	79	6.45e-14	62.8
MsG0180000074.01.T01	MTR_4g072370	47.826	69	32	2	13	77	12	80	7.22e-14	62.8
MsG0180000074.01.T01	MTR_4g072510	52.542	59	27	1	20	77	20	78	7.77e-14	62.8
MsG0180000074.01.T01	MTR_8g096440	42.857	70	40	0	22	91	46	115	8.42e-14	63.9
MsG0180000074.01.T01	MTR_3g110038	47.761	67	34	1	12	77	16	82	9.52e-14	62.8
MsG0180000074.01.T01	MTR_6g077850	40.580	69	40	1	19	86	32	100	9.85e-14	62.8
MsG0180000074.01.T01	MTR_8g096500	47.541	61	32	0	21	81	49	109	1.14e-13	63.9
MsG0180000074.01.T01	MTR_3g084220	50.746	67	32	1	12	77	16	82	1.76e-13	62.0
MsG0180000074.01.T01	MTR_4g072465	45.070	71	36	2	8	77	10	78	1.76e-13	61.6
MsG0180000074.01.T01	MTR_4g050700	50.000	60	29	1	19	77	24	83	1.94e-13	62.0
MsG0180000074.01.T01	MTR_2g012410	50.000	60	29	1	19	77	24	83	1.94e-13	62.0
MsG0180000074.01.T01	MTR_4g072490	46.377	69	34	2	10	77	11	77	2.35e-13	61.6
MsG0180000074.01.T01	MTR_4g072630	42.667	75	42	1	4	77	4	78	2.36e-13	61.6
MsG0180000074.01.T01	MTR_4g072550	49.275	69	31	2	19	86	34	99	3.42e-13	61.6
MsG0180000074.01.T01	MTR_4g072720	49.153	59	29	1	20	77	20	78	3.52e-13	60.8
MsG0180000074.01.T01	MTR_4g072380	41.558	77	44	1	4	79	4	80	4.10e-13	60.8
MsG0180000074.01.T01	MTR_5g008360	50.000	60	29	1	20	78	15	74	4.51e-13	60.8
MsG0180000074.01.T01	MTR_4g021810	43.243	74	41	1	21	93	26	99	5.68e-13	60.8
MsG0180000074.01.T01	MTR_4g072770	50.000	66	32	1	13	77	7	72	5.94e-13	60.5
MsG0180000074.01.T01	MTR_3g084260	43.836	73	40	1	6	77	11	83	1.12e-12	60.1
MsG0180000074.01.T01	MTR_4g072290	50.000	68	20	3	13	78	15	70	2.04e-12	58.9
MsG0180000074.01.T01	MTR_4g072560	43.590	78	36	4	3	77	18	90	2.72e-12	58.9
MsG0180000074.01.T01	MTR_4g072690	50.000	58	28	1	21	77	20	77	3.16e-12	58.5
MsG0180000074.01.T01	MTR_4g072450	48.276	58	29	1	21	77	20	77	3.40e-12	58.5
MsG0180000074.01.T01	MTR_8g022340	45.714	70	33	2	21	87	31	98	9.11e-12	58.2
MsG0180000074.01.T01	MTR_0388s0020	39.326	89	49	3	7	91	60	147	9.29e-12	58.9
MsG0180000074.01.T01	MTR_8g101520	37.500	80	50	0	22	101	51	130	1.01e-11	58.9
MsG0180000074.01.T01	MTR_5g008350	48.276	58	29	1	20	76	19	76	1.36e-11	56.6
MsG0180000074.01.T01	MTR_4g072470	41.333	75	42	2	5	77	3	77	1.58e-11	56.6
MsG0180000074.01.T01	MTR_4g072280	46.032	63	23	1	10	61	4	66	2.48e-11	56.2
MsG0180000074.01.T01	MTR_8g101530	32.979	94	62	1	7	99	25	118	3.24e-11	57.4
MsG0180000074.01.T01	MTR_7g101920	47.458	59	29	1	17	73	31	89	4.07e-11	56.2
MsG0180000074.01.T01	MTR_4g005310	41.429	70	39	1	14	81	55	124	7.19e-11	56.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180000074.01.T01	AT1G75590	62.222	135	46	3	1	130	20	154	1.65e-51	160
MsG0180000074.01.T01	AT1G19840	63.433	134	45	3	1	130	20	153	7.66e-51	159
MsG0180000074.01.T01	AT5G10990	60.769	130	47	2	1	129	20	146	1.22e-50	158
MsG0180000074.01.T01	AT4G34750	52.899	138	52	4	1	129	16	149	1.32e-43	140
MsG0180000074.01.T01	AT4G34750	52.899	138	52	4	1	129	16	149	1.32e-43	140
MsG0180000074.01.T01	AT4G34770	51.190	84	36	3	9	87	21	104	3.24e-19	77.0
MsG0180000074.01.T01	AT5G66260	44.706	85	47	0	1	85	14	98	3.61e-19	76.6
MsG0180000074.01.T01	AT4G34760	50.746	67	33	0	18	84	40	106	1.52e-17	72.8
MsG0180000074.01.T01	AT3G43120	44.737	76	42	0	18	93	76	151	1.00e-16	72.0
MsG0180000074.01.T01	AT2G18010	43.902	82	44	1	2	81	27	108	1.95e-16	70.1
MsG0180000074.01.T01	AT2G37030	48.529	68	35	0	14	81	42	109	2.07e-16	70.5
MsG0180000074.01.T01	AT2G21220	47.761	67	35	0	18	84	37	103	4.05e-16	69.3
MsG0180000074.01.T01	AT2G21210	58.333	60	22	2	21	77	28	87	4.09e-16	68.9
MsG0180000074.01.T01	AT5G20810	50.820	61	30	0	18	78	76	136	4.27e-16	70.5
MsG0180000074.01.T01	AT4G34800	57.971	69	23	3	14	77	14	81	4.44e-16	68.9
MsG0180000074.01.T01	AT1G75580	52.381	63	30	0	15	77	38	100	4.57e-16	68.9
MsG0180000074.01.T01	AT1G19830	47.059	68	36	0	14	81	41	108	7.31e-16	68.9
MsG0180000074.01.T01	AT2G16580	49.254	67	34	0	18	84	41	107	7.85e-16	68.6
MsG0180000074.01.T01	AT5G20810	50.000	64	32	0	18	81	76	139	7.87e-16	70.5
MsG0180000074.01.T01	AT1G79130	50.000	70	34	1	19	88	46	114	1.28e-15	68.6
MsG0180000074.01.T01	AT4G38840	58.333	60	23	2	20	77	32	91	1.31e-15	67.8
MsG0180000074.01.T01	AT2G21210	58.333	60	22	2	21	77	95	154	1.71e-15	68.9
MsG0180000074.01.T01	AT3G61900	44.444	81	42	1	12	89	21	101	2.30e-15	68.2
MsG0180000074.01.T01	AT1G56150	44.444	81	44	1	1	81	19	98	2.45e-15	67.4
MsG0180000074.01.T01	AT4G38860	54.098	61	27	1	18	77	37	97	3.96e-15	66.6
MsG0180000074.01.T01	AT4G36110	44.156	77	43	0	1	77	20	96	3.99e-15	66.6
MsG0180000074.01.T01	AT3G09870	48.611	72	35	1	12	81	36	107	5.72e-15	66.2
MsG0180000074.01.T01	AT3G53250	48.438	64	30	1	18	81	49	109	7.26e-15	66.6
MsG0180000074.01.T01	AT3G53250	48.438	64	30	1	18	81	80	140	1.29e-14	66.6
MsG0180000074.01.T01	AT1G16510	44.304	79	43	1	21	99	54	131	1.32e-14	66.2
MsG0180000074.01.T01	AT3G12830	45.588	68	37	0	14	81	43	110	1.75e-14	65.9
MsG0180000074.01.T01	AT2G46690	46.914	81	37	4	19	94	22	101	4.83e-14	64.3
MsG0180000074.01.T01	AT4G34810	57.143	63	20	3	21	77	48	109	5.29e-14	63.9
MsG0180000074.01.T01	AT4G34790	54.412	68	25	3	21	85	39	103	8.40e-14	63.2
MsG0180000074.01.T01	AT3G60690	45.455	66	33	1	18	80	85	150	1.38e-13	64.3
MsG0180000074.01.T01	AT4G00880	40.244	82	47	1	10	89	17	98	2.03e-13	62.8
MsG0180000074.01.T01	AT2G28085	51.667	60	25	1	17	72	51	110	2.69e-13	62.4
MsG0180000074.01.T01	AT4G38850	48.485	66	30	2	16	77	16	81	2.72e-13	61.6
MsG0180000074.01.T01	AT3G03820	47.143	70	31	3	13	77	19	87	6.02e-13	60.8
MsG0180000074.01.T01	AT5G53590	43.836	73	36	1	20	87	45	117	6.33e-13	62.0
MsG0180000074.01.T01	AT5G50760	42.857	70	39	1	13	81	46	115	6.70e-13	62.8
MsG0180000074.01.T01	AT1G76190	30.693	101	52	3	1	101	14	96	8.71e-13	61.2
MsG0180000074.01.T01	AT5G18020	45.833	72	37	2	8	77	11	82	9.34e-13	60.1
MsG0180000074.01.T01	AT5G18080	45.833	72	37	2	8	77	11	82	1.42e-12	59.7
MsG0180000074.01.T01	AT2G45210	40.698	86	47	2	9	91	67	151	1.76e-12	61.2
MsG0180000074.01.T01	AT4G13790	53.226	62	24	3	20	77	24	84	2.93e-12	58.9
MsG0180000074.01.T01	AT3G20220	40.845	71	41	1	12	81	38	108	3.37e-12	59.3
MsG0180000074.01.T01	AT3G51200	46.269	67	35	1	20	85	38	104	4.30e-12	58.9
MsG0180000074.01.T01	AT5G18010	44.444	72	38	2	8	77	11	82	4.48e-12	58.5
MsG0180000074.01.T01	AT4G31320	40.000	70	42	0	21	90	80	149	5.09e-12	60.5
MsG0180000074.01.T01	AT5G18050	44.444	72	38	2	8	77	11	82	5.15e-12	58.2
MsG0180000074.01.T01	AT2G24400	43.750	64	36	0	21	84	64	127	5.52e-12	60.1
MsG0180000074.01.T01	AT3G03840	42.647	68	36	2	13	77	19	86	8.82e-12	57.8
MsG0180000074.01.T01	AT5G18060	45.833	72	37	2	8	77	11	82	1.08e-11	57.4
MsG0180000074.01.T01	AT2G21200	41.463	82	44	3	1	80	3	82	1.61e-11	57.0
MsG0180000074.01.T01	AT4G22620	39.024	82	46	2	7	85	66	146	3.30e-11	57.8

Find 34 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTAGAACCAATGTGGATTTC+TGG	0.199338	1:-1125447	MsG0180000074.01.T01:CDS
AAACCGGTTTGATCCATTAT+TGG	0.234776	1:+1125501	None:intergenic
TCCCGGTCCGGTTCCAATAA+TGG	0.239344	1:-1125514	MsG0180000074.01.T01:CDS
TGGAGTAGCGGTCGAGATTC+AGG	0.313820	1:+1125424	None:intergenic
AGGCTCTATGGTTTATTTCC+CGG	0.326961	1:-1125531	MsG0180000074.01.T01:CDS
GTTCAAGCTGAAGAAGAGTA+TGG	0.364675	1:-1125607	MsG0180000074.01.T01:CDS
AATTCTTTGAAGAGGCTCTA+TGG	0.365452	1:-1125543	MsG0180000074.01.T01:CDS
CGTAGCACGCACCACAAATC+TGG	0.403520	1:+1125663	None:intergenic
CTATGGTTTATTTCCCGGTC+CGG	0.419615	1:-1125526	MsG0180000074.01.T01:CDS
GTTTGATCCATTATTGGAAC+CGG	0.423970	1:+1125507	None:intergenic
TCCATTATTGGAACCGGACC+GGG	0.440385	1:+1125513	None:intergenic
ATCCATTATTGGAACCGGAC+CGG	0.456254	1:+1125512	None:intergenic
GCCTCTGAAAATCTTCAAAC+CGG	0.457522	1:+1125485	None:intergenic
TCCGCCAGATGCTACGACGC+TGG	0.476188	1:-1125774	None:intergenic
GAGTATGGTTTCTCTAACCA+TGG	0.483440	1:-1125592	MsG0180000074.01.T01:CDS
AGCTAACTACACCAGATTTG+TGG	0.498015	1:-1125674	MsG0180000074.01.T01:CDS
TCAATTAGACTGCTACCAAA+TGG	0.499902	1:+1125384	None:intergenic
TGATGAAGAATTCTTTGAAG+AGG	0.530515	1:-1125551	MsG0180000074.01.T01:CDS
GCACCGTCAGATGTACCATC+TGG	0.535105	1:-1125718	MsG0180000074.01.T01:CDS
GTTCCAATAATGGATCAAAC+CGG	0.552422	1:-1125504	MsG0180000074.01.T01:CDS
GAATCTCGACCGCTACTCCA+TGG	0.566091	1:-1125421	MsG0180000074.01.T01:CDS
TTTCAGAGGCGTTGCCACGT+GGG	0.579188	1:-1125472	MsG0180000074.01.T01:CDS
TCAAAGAATTCTTCATCACA+AGG	0.582724	1:+1125556	None:intergenic
GGACACGTGGCAGTATGCGT+TGG	0.590985	1:-1125697	MsG0180000074.01.T01:CDS
AAGGGATAGTAAGCGGTCCA+TGG	0.600079	1:+1125575	None:intergenic
CATACTGCCACGTGTCCAGA+TGG	0.603315	1:+1125703	None:intergenic
TCACTTAGACCATGGAGTAG+CGG	0.625167	1:+1125412	None:intergenic
CAAAGAATTCTTCATCACAA+GGG	0.628701	1:+1125557	None:intergenic
TTCAGGCCAGAAATCCACAT+TGG	0.632379	1:+1125441	None:intergenic
TGTCCAGATGGTACATCTGA+CGG	0.655290	1:+1125715	None:intergenic
TCATCACAAGGGATAGTAAG+CGG	0.670139	1:+1125568	None:intergenic
CGTGGGAATTAGAACCAATG+TGG	0.671140	1:-1125455	MsG0180000074.01.T01:CDS
AGATGTACCATCTGGACACG+TGG	0.678220	1:-1125710	MsG0180000074.01.T01:CDS
CATTGGTTCTAATTCCCACG+TGG	0.701350	1:+1125458	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	CAAAGAATTCTTCATCACAA+GGG	+	Chr1:1125608-1125627	None:intergenic	30.0%
	GTCTAAGTGAAAAAACCATT+TGG	-	Chr1:1125763-1125782	MsG0180000074.01.T01:CDS	30.0%
	TCAAAGAATTCTTCATCACA+AGG	+	Chr1:1125609-1125628	None:intergenic	30.0%
!	AACCAAAAGCTTCTGAAAAA+TGG	+	Chr1:1125538-1125557	None:intergenic	30.0%
!	ACCAAAAGCTTCTGAAAAAT+GGG	+	Chr1:1125537-1125556	None:intergenic	30.0%
!	TGATGAAGAATTCTTTGAAG+AGG	-	Chr1:1125611-1125630	MsG0180000074.01.T01:CDS	30.0%
	GTTTGATCCATTATTGGAAC+CGG	+	Chr1:1125658-1125677	None:intergenic	35.0%
	TTAGAACCAATGTGGATTTC+TGG	-	Chr1:1125715-1125734	MsG0180000074.01.T01:CDS	35.0%
!	AATTCTTTGAAGAGGCTCTA+TGG	-	Chr1:1125619-1125638	MsG0180000074.01.T01:CDS	35.0%
!	CCAAAAGCTTCTGAAAAATG+GGG	+	Chr1:1125536-1125555	None:intergenic	35.0%
!!	AAACCGGTTTGATCCATTAT+TGG	+	Chr1:1125664-1125683	None:intergenic	35.0%
!!	GTTCCAATAATGGATCAAAC+CGG	-	Chr1:1125658-1125677	MsG0180000074.01.T01:CDS	35.0%
!!!	TGGTTTTTTCACTTAGACCA+TGG	+	Chr1:1125761-1125780	None:intergenic	35.0%
	AGCTAACTACACCAGATTTG+TGG	-	Chr1:1125488-1125507	MsG0180000074.01.T01:CDS	40.0%
	AGGCTCTATGGTTTATTTCC+CGG	-	Chr1:1125631-1125650	MsG0180000074.01.T01:CDS	40.0%
	GAGTATGGTTTCTCTAACCA+TGG	-	Chr1:1125570-1125589	MsG0180000074.01.T01:CDS	40.0%
	GTTCAAGCTGAAGAAGAGTA+TGG	-	Chr1:1125555-1125574	MsG0180000074.01.T01:CDS	40.0%
	TCATCACAAGGGATAGTAAG+CGG	+	Chr1:1125597-1125616	None:intergenic	40.0%
!	AAAGCTTCTGAAAAATGGGG+TGG	+	Chr1:1125533-1125552	None:intergenic	40.0%
!	GCCTCTGAAAATCTTCAAAC+CGG	+	Chr1:1125680-1125699	None:intergenic	40.0%
!!	CCCCATTTTTCAGAAGCTTT+TGG	-	Chr1:1125533-1125552	MsG0180000074.01.T01:CDS	40.0%
!!!	ACCGGTTTGAAGATTTTCAG+AGG	-	Chr1:1125676-1125695	MsG0180000074.01.T01:CDS	40.0%
	ATCCATTATTGGAACCGGAC+CGG	+	Chr1:1125653-1125672	None:intergenic	45.0%
	CGTGGGAATTAGAACCAATG+TGG	-	Chr1:1125707-1125726	MsG0180000074.01.T01:CDS	45.0%
	CTATGGTTTATTTCCCGGTC+CGG	-	Chr1:1125636-1125655	MsG0180000074.01.T01:CDS	45.0%
	TCACTTAGACCATGGAGTAG+CGG	+	Chr1:1125753-1125772	None:intergenic	45.0%
	TCTGAAAAATGGGGTGGTTC+AGG	+	Chr1:1125527-1125546	None:intergenic	45.0%
	TGTCCAGATGGTACATCTGA+CGG	+	Chr1:1125450-1125469	None:intergenic	45.0%
	TTCAGGCCAGAAATCCACAT+TGG	+	Chr1:1125724-1125743	None:intergenic	45.0%
!!	CATTGGTTCTAATTCCCACG+TGG	+	Chr1:1125707-1125726	None:intergenic	45.0%
	AAGGGATAGTAAGCGGTCCA+TGG	+	Chr1:1125590-1125609	None:intergenic	50.0%
	AGATGTACCATCTGGACACG+TGG	-	Chr1:1125452-1125471	MsG0180000074.01.T01:CDS	50.0%
	TCCATTATTGGAACCGGACC+GGG	+	Chr1:1125652-1125671	None:intergenic	50.0%
	CATACTGCCACGTGTCCAGA+TGG	+	Chr1:1125462-1125481	None:intergenic	55.0%
	CGTAGCACGCACCACAAATC+TGG	+	Chr1:1125502-1125521	None:intergenic	55.0%
	GAATCTCGACCGCTACTCCA+TGG	-	Chr1:1125741-1125760	MsG0180000074.01.T01:CDS	55.0%
	GCACCGTCAGATGTACCATC+TGG	-	Chr1:1125444-1125463	MsG0180000074.01.T01:CDS	55.0%
	TCCCGGTCCGGTTCCAATAA+TGG	-	Chr1:1125648-1125667	MsG0180000074.01.T01:CDS	55.0%
	TGGAGTAGCGGTCGAGATTC+AGG	+	Chr1:1125741-1125760	None:intergenic	55.0%
!!	TTTCAGAGGCGTTGCCACGT+GGG	-	Chr1:1125690-1125709	MsG0180000074.01.T01:CDS	55.0%
!!!	TTTTCAGAGGCGTTGCCACG+TGG	-	Chr1:1125689-1125708	MsG0180000074.01.T01:CDS	55.0%
	GGACACGTGGCAGTATGCGT+TGG	-	Chr1:1125465-1125484	MsG0180000074.01.T01:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	1125396	1125788	1125396	ID=MsG0180000074.01;Name=MsG0180000074.01
Chr1	mRNA	1125396	1125788	1125396	ID=MsG0180000074.01.T01;Parent=MsG0180000074.01;Name=MsG0180000074.01.T01;_AED=0.34;_eAED=0.34;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|130
Chr1	exon	1125396	1125788	1125396	ID=MsG0180000074.01.T01:exon:7423;Parent=MsG0180000074.01.T01
Chr1	CDS	1125396	1125788	1125396	ID=MsG0180000074.01.T01:cds;Parent=MsG0180000074.01.T01

Gene Sequence

>MsG0180000074.01.T01

ATGCTACGACGCTGGAGAAACAAAGCACGTATTTCATCAGCTAATCGTGCACCGTCAGATGTACCATCTGGACACGTGGCAGTATGCGTTGGAGCTAACTACACCAGATTTGTGGTGCGTGCTACGTACCTGAACCACCCCATTTTTCAGAAGCTTTTGGTTCAAGCTGAAGAAGAGTATGGTTTCTCTAACCATGGACCGCTTACTATCCCTTGTGATGAAGAATTCTTTGAAGAGGCTCTATGGTTTATTTCCCGGTCCGGTTCCAATAATGGATCAAACCGGTTTGAAGATTTTCAGAGGCGTTGCCACGTGGGAATTAGAACCAATGTGGATTTCTGGCCTGAATCTCGACCGCTACTCCATGGTCTAAGTGAAAAAACCATTTGGTAG

Protein sequence

>MsG0180000074.01.T01

MLRRWRNKARISSANRAPSDVPSGHVAVCVGANYTRFVVRATYLNHPIFQKLLVQAEEEYGFSNHGPLTIPCDEEFFEEALWFISRSGSNNGSNRFEDFQRRCHVGIRTNVDFWPESRPLLHGLSEKTIW*