AlfalfaGEDB | MsG0180000126.01

M. sativa cultivar ZhongmuNo.1 / MsG0180000126.01

KEGG: K10572
GO: GO:0005524 GO:0035299
PFAM: PF06090

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180000126.01.T01	XP_013466139.1	86.889	450	20	3	11	423	31	478	0	748

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180000126.01.T01	Q93YN9	47.153	439	190	4	12	412	1	435	3.01E-125	373

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180000126.01.T01	A0A072VDS9	86.889	450	20	3	11	423	31	478	0.0	748

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180000045.01	MsG0180000126.01	0.808782	2.615451e-50	1.508874e-47
MsG0180000126.01	MsG0480023978.01	0.810037	1.406345e-50	8.389024e-48

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PPI

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Gene1	Gene2	Type
MsG0180000126.01	MsG0380017792.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180000126.01.T01	MTR_1g022475	86.889	450	20	3	11	423	31	478	0.0	748
MsG0180000126.01.T01	MTR_1g022475	87.082	449	19	3	12	423	1	447	0.0	747
MsG0180000126.01.T01	MTR_3g112130	61.486	444	132	2	12	418	1	442	0.0	546
MsG0180000126.01.T01	MTR_3g112130	60.000	420	129	2	12	394	1	418	2.86e-174	494

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180000126.01.T01	AT5G42810	47.153	439	190	4	12	412	1	435	3.07e-126	373
MsG0180000126.01.T01	AT1G22100	47.489	438	184	6	13	412	3	432	6.73e-126	372
MsG0180000126.01.T01	AT1G58643	43.304	448	205	9	13	416	3	445	1.57e-109	330
MsG0180000126.01.T01	AT1G59312	43.304	448	205	9	13	416	3	445	1.57e-109	330
MsG0180000126.01.T01	AT1G58643	43.750	448	203	10	13	416	38	480	5.01e-109	330
MsG0180000126.01.T01	AT1G58936	43.750	448	203	10	13	416	38	480	5.01e-109	330
MsG0180000126.01.T01	AT1G59312	43.750	448	203	10	13	416	38	480	5.01e-109	330

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Find 98 sgRNAs with CRISPR-Local

Find 215 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATGGTTCATGGAAGAAA+TGG	0.432769	1:-1827780	MsG0180000126.01.T01:CDS
AAACATCAACTCGCCCAGCA+GGG	0.655838	1:+1829698	None:intergenic
AAATGGAGATGCAAACTTAT+AGG	0.304541	1:-1827763	MsG0180000126.01.T01:exon
AACCCACTCGATCTGTTCTC+TGG	0.426001	1:-1828732	MsG0180000126.01.T01:CDS
AACCGTCTCAAGGAATTCCT+TGG	0.514592	1:+1829744	None:intergenic
AAGTCTCAGTATGCAACTTC+AGG	0.393468	1:+1827738	None:intergenic
AATATTGTTCTCTCTTACAC+TGG	0.362819	1:-1831026	MsG0180000126.01.T01:intron
AATCAGCGTTGCAAGGAGTG+AGG	0.642103	1:-1830215	MsG0180000126.01.T01:CDS
AATGGAGATGCAAACTTATA+GGG	0.348706	1:-1827762	MsG0180000126.01.T01:exon
AATGGTTCTCTCATACTCGG+AGG	0.667393	1:-1828636	MsG0180000126.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAAACAGCCATTTGGTTAA+TGG	+	Chr1:1828656-1828675	None:intergenic	30.0%
AAAACAGCCATTTGGTTAAT+GGG	+	Chr1:1828655-1828674	None:intergenic	30.0%
AAAATGGTTCATGGAAGAAA+TGG	-	Chr1:1830824-1830843	MsG0180000126.01.T01:intron	30.0%
AAATGGAGATGCAAACTTAT+AGG	-	Chr1:1830841-1830860	MsG0180000126.01.T01:intron	30.0%
AAGAATTACATAAGAAGGAG+AGG	+	Chr1:1827606-1827625	None:intergenic	30.0%
AATATTGTTCTCTCTTACAC+TGG	-	Chr1:1827578-1827597	MsG0180000126.01.T01:three_prime_UTR	30.0%
AATGGAGATGCAAACTTATA+GGG	-	Chr1:1830842-1830861	MsG0180000126.01.T01:intron	30.0%
ACTTATTTCCTCTTCAGATA+AGG	-	Chr1:1828465-1828484	MsG0180000126.01.T01:CDS	30.0%
AGACAAATGATAAAATGCTC+AGG	+	Chr1:1829752-1829771	None:intergenic	30.0%
AGTAGTAAAGAGATCTTTGA+TGG	+	Chr1:1829921-1829940	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	1827482	1831144	1827482	ID=MsG0180000126.01;Name=MsG0180000126.01
Chr1	mRNA	1827482	1831144	1827482	ID=MsG0180000126.01.T01;Parent=MsG0180000126.01;Name=MsG0180000126.01.T01;_AED=0.49;_eAED=0.51;_QI=0\|0.6\|0.66\|1\|1\|1\|6\|282\|423
Chr1	exon	1831027	1831144	1831027	ID=MsG0180000126.01.T01:exon:6990;Parent=MsG0180000126.01.T01
Chr1	exon	1830068	1830300	1830068	ID=MsG0180000126.01.T01:exon:6991;Parent=MsG0180000126.01.T01
Chr1	exon	1829635	1829782	1829635	ID=MsG0180000126.01.T01:exon:6992;Parent=MsG0180000126.01.T01
Chr1	exon	1829209	1829249	1829209	ID=MsG0180000126.01.T01:exon:6993;Parent=MsG0180000126.01.T01
Chr1	exon	1828179	1828775	1828179	ID=MsG0180000126.01.T01:exon:6994;Parent=MsG0180000126.01.T01
Chr1	exon	1827482	1827898	1827482	ID=MsG0180000126.01.T01:exon:6995;Parent=MsG0180000126.01.T01
Chr1	CDS	1831027	1831144	1831027	ID=MsG0180000126.01.T01:cds;Parent=MsG0180000126.01.T01
Chr1	CDS	1830068	1830300	1830068	ID=MsG0180000126.01.T01:cds;Parent=MsG0180000126.01.T01
Chr1	CDS	1829635	1829782	1829635	ID=MsG0180000126.01.T01:cds;Parent=MsG0180000126.01.T01
Chr1	CDS	1829209	1829249	1829209	ID=MsG0180000126.01.T01:cds;Parent=MsG0180000126.01.T01
Chr1	CDS	1828179	1828775	1828179	ID=MsG0180000126.01.T01:cds;Parent=MsG0180000126.01.T01
Chr1	CDS	1827764	1827898	1827764	ID=MsG0180000126.01.T01:cds;Parent=MsG0180000126.01.T01
Chr1	three_prime_UTR	1827482	1827763	1827482	ID=MsG0180000126.01.T01:three_prime_utr;Parent=MsG0180000126.01.T01

Gene Sequence

>MsG0180000126.01.T01

ATGCCTGACAAAGATTTTCATGTGAAAAAACAGATGGAGTTGACTTTAACTGAAGAAGATGCAGCTCATTGGGTTTATAGAGGTGAAGGTGCTGCTAATATTGTTCTCTCTTACACTGAATATCCAAATGTGTCTTACTTAGATTGGAAAGTGATGCGCATTCGCAAGTCTCCAAGGAAGGAATCAGCGTTGCAAGGAGTGAGGAATACCATCGCTCTGACTCCACATGAACGGCTCATCTGGAAAAATGTACCTGAACTTATTTCCTCTTCAGATAAGGAAATTGCTGGGCAACTATATGTAGAGCATGTCATGAAGCCTTTGCTTGGTTCCAAATATGTTGATGCTGGGACTCACATTTTGGTGACCAAGGAATTCCTTGAGACGGTTGAGAAGAATGTTGCTAGTCAACGCCCTGCTGGGCGAGTTGATGTTTCAAGGGTTGATACACAATGTGATTTTAGTCTTCTCATGTCGGATCATTCAATCTTCCCTCAAGGTAGTCAAGGATCTATCCCCAGCTTATCAGTTGAGATAAAGATTTCAGAGCGGAGTGTGTACAACCCACTCGATCTGTTCTCTGGATCCAAAGAAAGAATTCATAAAGCCATCAAAGATCTCTTTACTACTCCTCAAAACAATTTCCGTGTATTTTTAAATGGTTCTCTCATACTCGGAGGGCTTGGAGGTGGTGCTGAAAGTACAGATGCATCTATGGCTAAAGTACTTGAAGATGAACTTCAGCCGGTCATTCAAGCTGATGATGGCCAATGCACAGAGAACTTATTTACTCTTGTTACTGAAGCCGTGCAAAAATCAGGAGTCCTTGATAAGCTCCTTGAGGTTCAGAAGCTTGACAATATTGACATAGAAGGAGTAATTCATGCATATTACAACATTACTTCTCAACAGAGCATGGCGCGTAAAGAATTGGATGAAGAACAAGCAAAAAAATATGCCTCTTTGCATTCTGCATCACTGGAGGAAAGCTTGAGAATTGTAAAGGACTACCTGATAGCAGCAACTGCAAAAGACTGCAGTTTGATGATATGTTTTAGACCAAGGAAAGAGGAGGGTTCTGGATCTGCCTACGATACTGTATATCTTGAGTCAACTAAGCAAGCCTTTGATTTTAAGGTGCATTTTATTGACCTCGATTTAAAACGGTTGAATAAAGTGGAAGACTACCACAAGTTAGATAAGAAGATAGTGAACTGTTACAAAGAGATGAGCAAAATGGTTCATGGAAGAAATGGAGATGCAAACTTATAG

Protein sequence

>MsG0180000126.01.T01

MPDKDFHVKKQMELTLTEEDAAHWVYRGEGAANIVLSYTEYPNVSYLDWKVMRIRKSPRKESALQGVRNTIALTPHERLIWKNVPELISSSDKEIAGQLYVEHVMKPLLGSKYVDAGTHILVTKEFLETVEKNVASQRPAGRVDVSRVDTQCDFSLLMSDHSIFPQGSQGSIPSLSVEIKISERSVYNPLDLFSGSKERIHKAIKDLFTTPQNNFRVFLNGSLILGGLGGGAESTDASMAKVLEDELQPVIQADDGQCTENLFTLVTEAVQKSGVLDKLLEVQKLDNIDIEGVIHAYYNITSQQSMARKELDEEQAKKYASLHSASLEESLRIVKDYLIAATAKDCSLMICFRPRKEEGSGSAYDTVYLESTKQAFDFKVHFIDLDLKRLNKVEDYHKLDKKIVNCYKEMSKMVHGRNGDANL*