AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180000126.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180000126.01.T01 MTR_1g022475 86.889 450 20 3 11 423 31 478 0.0 748
MsG0180000126.01.T01 MTR_1g022475 87.082 449 19 3 12 423 1 447 0.0 747
MsG0180000126.01.T01 MTR_3g112130 61.486 444 132 2 12 418 1 442 0.0 546
MsG0180000126.01.T01 MTR_3g112130 60.000 420 129 2 12 394 1 418 2.86e-174 494
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180000126.01.T01 AT5G42810 47.153 439 190 4 12 412 1 435 3.07e-126 373
MsG0180000126.01.T01 AT1G22100 47.489 438 184 6 13 412 3 432 6.73e-126 372
MsG0180000126.01.T01 AT1G58643 43.304 448 205 9 13 416 3 445 1.57e-109 330
MsG0180000126.01.T01 AT1G59312 43.304 448 205 9 13 416 3 445 1.57e-109 330
MsG0180000126.01.T01 AT1G58643 43.750 448 203 10 13 416 38 480 5.01e-109 330
MsG0180000126.01.T01 AT1G58936 43.750 448 203 10 13 416 38 480 5.01e-109 330
MsG0180000126.01.T01 AT1G59312 43.750 448 203 10 13 416 38 480 5.01e-109 330

Find 98 sgRNAs with CRISPR-Local

Find 215 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACTTTCTAGACTCACATTT+TGG 0.211675 1:-1829770 MsG0180000126.01.T01:intron
GGCTTTATGAATTCTTTCTT+TGG 0.242124 1:+1828707 None:intergenic
TACCCCAGCATCAACATATT+TGG 0.257064 1:+1830065 None:intergenic
AAATGGAGATGCAAACTTAT+AGG 0.304541 1:-1827763 MsG0180000126.01.T01:exon
CTCCACATGAACGGCTCATC+TGG 0.312520 1:-1830176 MsG0180000126.01.T01:CDS
CAAATGTGTCTTACTTAGAT+TGG 0.318709 1:-1830272 MsG0180000126.01.T01:CDS
CAGCTTATCAGTTGAGATAA+AGG 0.331799 1:-1829208 MsG0180000126.01.T01:intron
TGCAACGCTGATTCCTTCCT+TGG 0.337708 1:+1830225 None:intergenic
AATGGAGATGCAAACTTATA+GGG 0.348706 1:-1827762 MsG0180000126.01.T01:exon
GTTGCTAGTCAACGCCCTGC+TGG 0.357402 1:-1829712 MsG0180000126.01.T01:CDS
CCAAGGAAAGAGGAGGGTTC+TGG 0.359741 1:-1828234 MsG0180000126.01.T01:CDS
AATATTGTTCTCTCTTACAC+TGG 0.362819 1:-1831026 MsG0180000126.01.T01:intron
CAATCTAAGTAAGACACATT+TGG 0.365192 1:+1830273 None:intergenic
CTGGGCGAGTTGATGTTTCA+AGG 0.365906 1:-1829693 MsG0180000126.01.T01:CDS
CTCTTTGCATTCTGCATCAC+TGG 0.393035 1:-1828334 MsG0180000126.01.T01:CDS
AAGTCTCAGTATGCAACTTC+AGG 0.393468 1:+1827738 None:intergenic
TTCACATGAAAATCTTTGTC+AGG 0.394181 1:+1831119 None:intergenic
CCAGAACCCTCCTCTTTCCT+TGG 0.396286 1:+1828234 None:intergenic
GAGCATGGCGCGTAAAGAAT+TGG 0.400860 1:-1828382 MsG0180000126.01.T01:CDS
AGTAGTAAAGAGATCTTTGA+TGG 0.402587 1:+1828686 None:intergenic
CTTTATCTCAACTGATAAGC+TGG 0.408033 1:+1829209 None:intergenic
GGTTCCAAATATGTTGATGC+TGG 0.410935 1:-1830069 MsG0180000126.01.T01:CDS
TTTATGAGTTATCATATATG+AGG 0.412078 1:+1827499 None:intergenic
TGAAGAAGATGCAGCTCATT+GGG 0.412372 1:-1831072 MsG0180000126.01.T01:CDS
CTGAAGAAGATGCAGCTCAT+TGG 0.417078 1:-1831073 MsG0180000126.01.T01:CDS
TCTCTCATACTCGGAGGGCT+TGG 0.418888 1:-1828630 MsG0180000126.01.T01:CDS
TGAAAGTACAGATGCATCTA+TGG 0.423587 1:-1828598 MsG0180000126.01.T01:CDS
AACCCACTCGATCTGTTCTC+TGG 0.426001 1:-1828732 MsG0180000126.01.T01:CDS
ATATATGAGGAAACAAATAA+TGG 0.426540 1:+1827512 None:intergenic
CTCATACTCGGAGGGCTTGG+AGG 0.427893 1:-1828627 MsG0180000126.01.T01:CDS
CTACCCTCCTCACATGCTAT+TGG 0.428346 1:-1827635 MsG0180000126.01.T01:three_prime_UTR
TTACAAAGAGATGAGCAAAA+TGG 0.430043 1:-1827796 MsG0180000126.01.T01:CDS
AAAATGGTTCATGGAAGAAA+TGG 0.432769 1:-1827780 MsG0180000126.01.T01:CDS
TCTTCAGATAAGGAAATTGC+TGG 0.438795 1:-1830129 MsG0180000126.01.T01:CDS
CAAAATAATTATGCAAAAGA+TGG 0.440358 1:+1827572 None:intergenic
TATACAGAAAATTGATAGTT+GGG 0.446477 1:+1827611 None:intergenic
GAGATGAGCAAAATGGTTCA+TGG 0.451783 1:-1827789 MsG0180000126.01.T01:CDS
CATGTCATGAAGCCTTTGCT+TGG 0.452650 1:-1830090 MsG0180000126.01.T01:CDS
CTTGACAATATTGACATAGA+AGG 0.455818 1:-1828441 MsG0180000126.01.T01:CDS
ACTTATTTCCTCTTCAGATA+AGG 0.460694 1:-1830139 MsG0180000126.01.T01:CDS
TTTAAAACGGTTGAATAAAG+TGG 0.467422 1:-1827856 MsG0180000126.01.T01:CDS
GAAACATCAACTCGCCCAGC+AGG 0.473865 1:+1829697 None:intergenic
GTATACAGAAAATTGATAGT+TGG 0.485821 1:+1827610 None:intergenic
TCTGCTTACCTTAAAATCAA+AGG 0.487614 1:+1828170 None:intergenic
GCAGCTCATTGGGTTTATAG+AGG 0.490643 1:-1831062 MsG0180000126.01.T01:CDS
CCATCATCAGCTTGAATGAC+CGG 0.498277 1:+1828549 None:intergenic
TTATTGACCTCGATTTAAAA+CGG 0.501967 1:-1827869 MsG0180000126.01.T01:CDS
GTGAAGCAAAATATTTATGC+AGG 0.504797 1:+1827657 None:intergenic
TCTGAAGAGGAAATAAGTTC+AGG 0.504962 1:+1830144 None:intergenic
AGCAATTTCCTTATCTGAAG+AGG 0.506496 1:+1830131 None:intergenic
AACCGTCTCAAGGAATTCCT+TGG 0.514592 1:+1829744 None:intergenic
GGAAAGCTTGAGAATTGTAA+AGG 0.520521 1:-1828310 MsG0180000126.01.T01:CDS
GTCTCAAAACCATACCTTGA+GGG 0.520824 1:+1829620 None:intergenic
TTGCTAGTCAACGCCCTGCT+GGG 0.522802 1:-1829711 MsG0180000126.01.T01:CDS
TTAAATGGTTCTCTCATACT+CGG 0.530482 1:-1828639 MsG0180000126.01.T01:CDS
TTTATCTCAACTGATAAGCT+GGG 0.534076 1:+1829210 None:intergenic
GTTCCAAATATGTTGATGCT+GGG 0.535103 1:-1830068 MsG0180000126.01.T01:CDS
GAACCTCAAGGAGCTTATCA+AGG 0.536139 1:+1828469 None:intergenic
CCGGTCATTCAAGCTGATGA+TGG 0.539433 1:-1828549 MsG0180000126.01.T01:CDS
TAAATAAGTTCTCTGTGCAT+TGG 0.540290 1:+1828526 None:intergenic
ATACTCGGAGGGCTTGGAGG+TGG 0.540855 1:-1828624 MsG0180000126.01.T01:CDS
CATTCTTCTCAACCGTCTCA+AGG 0.543125 1:+1829734 None:intergenic
CATTGGGTTTATAGAGGTGA+AGG 0.550198 1:-1831056 MsG0180000126.01.T01:CDS
TGTTTCATTTCAGGTAGTCA+AGG 0.559197 1:-1829240 MsG0180000126.01.T01:intron
CTATCTTCTTATCTAACTTG+TGG 0.562101 1:+1827826 None:intergenic
ATGAGGAAACAAATAATGGA+AGG 0.577913 1:+1827516 None:intergenic
ACTTGAAGATGAACTTCAGC+CGG 0.579402 1:-1828568 MsG0180000126.01.T01:CDS
ATGGTTCTCTCATACTCGGA+GGG 0.583585 1:-1828635 MsG0180000126.01.T01:CDS
ACATATTTGGAACCAAGCAA+AGG 0.590042 1:+1830078 None:intergenic
TGGGCGAGTTGATGTTTCAA+GGG 0.590970 1:-1829692 MsG0180000126.01.T01:CDS
CATTCGCAAGTCTCCAAGGA+AGG 0.599728 1:-1830238 MsG0180000126.01.T01:CDS
TTTGCATTCTGCATCACTGG+AGG 0.601325 1:-1828331 MsG0180000126.01.T01:CDS
TTCCAGATGAGCCGTTCATG+TGG 0.604622 1:+1830174 None:intergenic
TGCGCATTCGCAAGTCTCCA+AGG 0.605257 1:-1830242 MsG0180000126.01.T01:CDS
GACCAAGGAATTCCTTGAGA+CGG 0.607660 1:-1829746 MsG0180000126.01.T01:CDS
GATCATTCAATCTTCCCTCA+AGG 0.608947 1:-1829634 MsG0180000126.01.T01:intron
AGGAAGGAATCAGCGTTGCA+AGG 0.612808 1:-1830222 MsG0180000126.01.T01:CDS
CTCAAGATATACAGTATCGT+AGG 0.616260 1:+1828206 None:intergenic
CATTACTTCTCAACAGAGCA+TGG 0.616815 1:-1828397 MsG0180000126.01.T01:CDS
TTTAGACCAAGGAAAGAGGA+GGG 0.622681 1:-1828240 MsG0180000126.01.T01:CDS
CTTCAGATAAGGAAATTGCT+GGG 0.625703 1:-1830128 MsG0180000126.01.T01:CDS
ATGCAAAAGATGGATTCTGA+TGG 0.626365 1:+1827582 None:intergenic
GTTGGGACCAATAGCATGTG+AGG 0.633773 1:+1827628 None:intergenic
TGTCAAGCTTCTGAACCTCA+AGG 0.634666 1:+1828457 None:intergenic
GGACCAATAGCATGTGAGGA+GGG 0.635570 1:+1827632 None:intergenic
GATCCAGAGAACAGATCGAG+TGG 0.637943 1:+1828729 None:intergenic
TTCATGTGGAGTCAGAGCGA+TGG 0.639638 1:+1830188 None:intergenic
AATCAGCGTTGCAAGGAGTG+AGG 0.642103 1:-1830215 MsG0180000126.01.T01:CDS
CAGTGATGCAGAATGCAAAG+AGG 0.642291 1:+1828335 None:intergenic
AGTCCTTGATAAGCTCCTTG+AGG 0.646197 1:-1828472 MsG0180000126.01.T01:CDS
GGGACCAATAGCATGTGAGG+AGG 0.649926 1:+1827631 None:intergenic
TTCCAAATATGTTGATGCTG+GGG 0.654226 1:-1830067 MsG0180000126.01.T01:intron
AAACATCAACTCGCCCAGCA+GGG 0.655838 1:+1829698 None:intergenic
TCGCTCTGACTCCACATGAA+CGG 0.660327 1:-1830185 MsG0180000126.01.T01:CDS
TTATCTCAACTGATAAGCTG+GGG 0.664084 1:+1829211 None:intergenic
AATGGTTCTCTCATACTCGG+AGG 0.667393 1:-1828636 MsG0180000126.01.T01:CDS
ATCCAGAGAACAGATCGAGT+GGG 0.685169 1:+1828730 None:intergenic
TATGTCTTCAGATTTCAGAG+CGG 0.776737 1:-1828764 MsG0180000126.01.T01:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTTTTCTATTGATAAAAA+CGG - Chr1:1828715-1828734 MsG0180000126.01.T01:CDS 10.0%
!!! TCAAAAAATGTTTTTTTTTA+AGG - Chr1:1827673-1827692 MsG0180000126.01.T01:three_prime_UTR 10.0%
!!! TTATTATTGGTTTAAATTTT+TGG - Chr1:1830469-1830488 MsG0180000126.01.T01:intron 10.0%
!! AAAAAAAGAATTACATAAGA+AGG + Chr1:1827611-1827630 None:intergenic 15.0%
!! AAACTAATGATATTCTTTAA+TGG + Chr1:1829467-1829486 None:intergenic 15.0%
!! AAATTTCCAGTTATTATTAT+TGG - Chr1:1830456-1830475 MsG0180000126.01.T01:intron 15.0%
!! AACTAATGATATTCTTTAAT+GGG + Chr1:1829466-1829485 None:intergenic 15.0%
!! TTTAAACCAATAATAATAAC+TGG + Chr1:1830465-1830484 None:intergenic 15.0%
!!! CTTTATTTTGTCTTTTATTA+TGG - Chr1:1828219-1828238 MsG0180000126.01.T01:CDS 15.0%
!!! GTTTTTATCAATAGAAAAAT+TGG + Chr1:1828716-1828735 None:intergenic 15.0%
!!! TTCTTATGTAATTCTTTTTT+TGG - Chr1:1827610-1827629 MsG0180000126.01.T01:three_prime_UTR 15.0%
!! AAAGACAAAATAAAGTCAAA+CGG + Chr1:1828214-1828233 None:intergenic 20.0%
!! ACTTCTTATTCGTAAAAATA+GGG + Chr1:1828754-1828773 None:intergenic 20.0%
!! ATATATGAGGAAACAAATAA+TGG + Chr1:1831095-1831114 None:intergenic 20.0%
!! CAAAATAATTATGCAAAAGA+TGG + Chr1:1831035-1831054 None:intergenic 20.0%
!! CATATTGAAAAGTTTCTAAT+AGG + Chr1:1829824-1829843 None:intergenic 20.0%
!! CTACAAAATTTAACTATAGA+TGG - Chr1:1829694-1829713 MsG0180000126.01.T01:CDS 20.0%
!! TACTTCTTATTCGTAAAAAT+AGG + Chr1:1828755-1828774 None:intergenic 20.0%
!! TTGGTTAAAATATGAACATT+GGG - Chr1:1829174-1829193 MsG0180000126.01.T01:intron 20.0%
!! TTTATGAGTTATCATATATG+AGG + Chr1:1831108-1831127 None:intergenic 20.0%
!!! AATGTTCATATTTTAACCAA+CGG + Chr1:1829174-1829193 None:intergenic 20.0%
!!! AATTTCCGTGTATTTTTAAA+TGG - Chr1:1829950-1829969 MsG0180000126.01.T01:intron 20.0%
!!! ACAAGAACTTTTCTATTAAT+AGG + Chr1:1828612-1828631 None:intergenic 20.0%
!!! ACTTTTTGTAGATAAGATAA+GGG + Chr1:1830645-1830664 None:intergenic 20.0%
!!! ATATGTCTATAAACTGTTTT+CGG - Chr1:1827932-1827951 MsG0180000126.01.T01:intron 20.0%
!!! CATCTATAGTTAAATTTTGT+AGG + Chr1:1829696-1829715 None:intergenic 20.0%
!!! TACTTTTTGTAGATAAGATA+AGG + Chr1:1830646-1830665 None:intergenic 20.0%
!!! TATACAGAAAATTGATAGTT+GGG + Chr1:1830996-1831015 None:intergenic 20.0%
! ACTTGTGATATTATTTGGAA+AGG - Chr1:1827890-1827909 MsG0180000126.01.T01:intron 25.0%
! CAAAATTTAACTATAGATGG+TGG - Chr1:1829697-1829716 MsG0180000126.01.T01:CDS 25.0%
! GAATTAGTTAAGAACCAATT+AGG - Chr1:1828019-1828038 MsG0180000126.01.T01:intron 25.0%
! GAGAACCATTTAAAAATACA+CGG + Chr1:1829958-1829977 None:intergenic 25.0%
! GCAATCATACAAAATTCTAT+TGG + Chr1:1827800-1827819 None:intergenic 25.0%
! GGATATTCTGTTTAATTGTT+TGG + Chr1:1828313-1828332 None:intergenic 25.0%
! GTTGGTTAAAATATGAACAT+TGG - Chr1:1829173-1829192 MsG0180000126.01.T01:intron 25.0%
! TAATCTCACAAGCATTATAA+CGG + Chr1:1830672-1830691 None:intergenic 25.0%
! TAATGATATTCTTTAATGGG+AGG + Chr1:1829463-1829482 None:intergenic 25.0%
! TATCACAAAAACATTCTGTA+AGG + Chr1:1830904-1830923 None:intergenic 25.0%
! TGAGATTAAATGTCTTAGTT+CGG - Chr1:1830684-1830703 MsG0180000126.01.T01:intron 25.0%
! TGGATTGTAAAATTCATGTA+TGG - Chr1:1827759-1827778 MsG0180000126.01.T01:exon 25.0%
! TTATTGACCTCGATTTAAAA+CGG - Chr1:1830735-1830754 MsG0180000126.01.T01:intron 25.0%
!! GAATATGTTCAGTTCATTTT+TGG - Chr1:1830509-1830528 MsG0180000126.01.T01:intron 25.0%
!! GTATACAGAAAATTGATAGT+TGG + Chr1:1830997-1831016 None:intergenic 25.0%
!! TTTAAAACGGTTGAATAAAG+TGG - Chr1:1830748-1830767 MsG0180000126.01.T01:intron 25.0%
!! TTTTCATGTGAAAAAACAGA+TGG - Chr1:1827496-1827515 MsG0180000126.01.T01:three_prime_UTR 25.0%
!!! ATGTTCATATTTTAACCAAC+GGG + Chr1:1829173-1829192 None:intergenic 25.0%
!!! CTTGTTTTATGTTTCATGTT+TGG - Chr1:1827739-1827758 MsG0180000126.01.T01:three_prime_UTR 25.0%
!!! GGATTTAGTGTGTTTTTATA+CGG - Chr1:1830592-1830611 MsG0180000126.01.T01:intron 25.0%
AAAAACAGCCATTTGGTTAA+TGG + Chr1:1828656-1828675 None:intergenic 30.0%
AAAACAGCCATTTGGTTAAT+GGG + Chr1:1828655-1828674 None:intergenic 30.0%
AAAATGGTTCATGGAAGAAA+TGG - Chr1:1830824-1830843 MsG0180000126.01.T01:intron 30.0%
AAATGGAGATGCAAACTTAT+AGG - Chr1:1830841-1830860 MsG0180000126.01.T01:intron 30.0%
AAGAATTACATAAGAAGGAG+AGG + Chr1:1827606-1827625 None:intergenic 30.0%
AATATTGTTCTCTCTTACAC+TGG - Chr1:1827578-1827597 MsG0180000126.01.T01:three_prime_UTR 30.0%
AATGGAGATGCAAACTTATA+GGG - Chr1:1830842-1830861 MsG0180000126.01.T01:intron 30.0%
ACTTATTTCCTCTTCAGATA+AGG - Chr1:1828465-1828484 MsG0180000126.01.T01:CDS 30.0%
AGACAAATGATAAAATGCTC+AGG + Chr1:1829752-1829771 None:intergenic 30.0%
AGTAGTAAAGAGATCTTTGA+TGG + Chr1:1829921-1829940 None:intergenic 30.0%
ATCAAAATGTAGCCTAAATG+CGG - Chr1:1829564-1829583 MsG0180000126.01.T01:intron 30.0%
ATGAACTGAACATATTCGTA+TGG + Chr1:1830506-1830525 None:intergenic 30.0%
CAAATGTGTCTTACTTAGAT+TGG - Chr1:1828332-1828351 MsG0180000126.01.T01:CDS 30.0%
CAATCTAAGTAAGACACATT+TGG + Chr1:1828334-1828353 None:intergenic 30.0%
CCAAGTGTTAAATTGATTGA+AGG + Chr1:1830574-1830593 None:intergenic 30.0%
CCTTCAATCAATTTAACACT+TGG - Chr1:1830571-1830590 MsG0180000126.01.T01:intron 30.0%
CTATCTTCTTATCTAACTTG+TGG + Chr1:1830781-1830800 None:intergenic 30.0%
CTTGACAATATTGACATAGA+AGG - Chr1:1830163-1830182 MsG0180000126.01.T01:CDS 30.0%
GTGAAGCAAAATATTTATGC+AGG + Chr1:1830950-1830969 None:intergenic 30.0%
GTTAGAGATTTAGACTCAAA+GGG + Chr1:1828119-1828138 None:intergenic 30.0%
TAAATAAGTTCTCTGTGCAT+TGG + Chr1:1830081-1830100 None:intergenic 30.0%
TAATTCATGATTTCTGAGCA+AGG + Chr1:1829087-1829106 None:intergenic 30.0%
TATCAATTCTGTCAGTAGTT+AGG - Chr1:1827997-1828016 MsG0180000126.01.T01:intron 30.0%
TATCAGTTGAGATAAAGGTA+AGG - Chr1:1829401-1829420 MsG0180000126.01.T01:intron 30.0%
TCAGATATGAATCTTGAAGT+AGG + Chr1:1829597-1829616 None:intergenic 30.0%
TCATACTAGGTAAATACTAC+CGG + Chr1:1829263-1829282 None:intergenic 30.0%
TCTGCTTACCTTAAAATCAA+AGG + Chr1:1830437-1830456 None:intergenic 30.0%
TGATGATAAAAACAGCCATT+TGG + Chr1:1828663-1828682 None:intergenic 30.0%
TGCTAAGACAAATGCAATAT+AGG + Chr1:1829724-1829743 None:intergenic 30.0%
TGTTAGAGATTTAGACTCAA+AGG + Chr1:1828120-1828139 None:intergenic 30.0%
TTAAATGGTTCTCTCATACT+CGG - Chr1:1829965-1829984 MsG0180000126.01.T01:intron 30.0%
TTACAAAGAGATGAGCAAAA+TGG - Chr1:1830808-1830827 MsG0180000126.01.T01:intron 30.0%
TTAGGAGTTAGTTACTCAAT+TGG - Chr1:1828037-1828056 MsG0180000126.01.T01:intron 30.0%
TTCACATGAAAATCTTTGTC+AGG + Chr1:1827488-1827507 None:intergenic 30.0%
TTGAAAACCCATTAACCAAA+TGG - Chr1:1828645-1828664 MsG0180000126.01.T01:CDS 30.0%
TTGAGTAACTAACTCCTAAT+TGG + Chr1:1828036-1828055 None:intergenic 30.0%
TTTATCTCAACTGATAAGCT+GGG + Chr1:1829397-1829416 None:intergenic 30.0%
TTTCATAAGTCTCAGTTACT+GGG + Chr1:1828168-1828187 None:intergenic 30.0%
! ATGAGGAAACAAATAATGGA+AGG + Chr1:1831091-1831110 None:intergenic 30.0%
! GAAGCACTTGTGATATTATT+TGG - Chr1:1827885-1827904 MsG0180000126.01.T01:intron 30.0%
! GGCTTTATGAATTCTTTCTT+TGG + Chr1:1829900-1829919 None:intergenic 30.0%
! TACTTTCTAGACTCACATTT+TGG - Chr1:1828834-1828853 MsG0180000126.01.T01:intron 30.0%
! TGATCAGTTTAGTTCATACT+AGG + Chr1:1829276-1829295 None:intergenic 30.0%
! TTTTCATAAGTCTCAGTTAC+TGG + Chr1:1828169-1828188 None:intergenic 30.0%
!! AATACACGGAAATTGTTTTG+AGG + Chr1:1829944-1829963 None:intergenic 30.0%
!! GCTTAGTTCTAGAAAAATGA+CGG + Chr1:1829802-1829821 None:intergenic 30.0%
!! GTTGCTACTTGATTTGTATT+AGG - Chr1:1830706-1830725 MsG0180000126.01.T01:intron 30.0%
!! TAAGCAAGCCTTTGATTTTA+AGG - Chr1:1830426-1830445 MsG0180000126.01.T01:intron 30.0%
!! TATTCAACCGTTTTAAATCG+AGG + Chr1:1830745-1830764 None:intergenic 30.0%
!! TGATGATATGTTTTAGACCA+AGG - Chr1:1830353-1830372 MsG0180000126.01.T01:intron 30.0%
!! TGATTTTAGTCTTCTCATGT+CGG - Chr1:1828948-1828967 MsG0180000126.01.T01:intron 30.0%
!! TTAGGCTACATTTTGATGAA+TGG + Chr1:1829561-1829580 None:intergenic 30.0%
!!! TCAGTTCATTTTTGGTTAGT+AGG - Chr1:1830517-1830536 MsG0180000126.01.T01:intron 30.0%
!!! TGGTTTAAATTTTTGGTGTC+TGG - Chr1:1830476-1830495 MsG0180000126.01.T01:intron 30.0%
AAATCAAGCCAAAGACAAAG+AGG + Chr1:1829523-1829542 None:intergenic 35.0%
AGAACAGGAGAAGAATGAAA+CGG + Chr1:1828573-1828592 None:intergenic 35.0%
AGCAATTTCCTTATCTGAAG+AGG + Chr1:1828476-1828495 None:intergenic 35.0%
AGTAAAGATGTTGCATGAGT+AGG - Chr1:1828771-1828790 MsG0180000126.01.T01:intron 35.0%
ATTAATTCCGTAAGCTCTTC+AGG - Chr1:1827955-1827974 MsG0180000126.01.T01:intron 35.0%
CAATGTAATTCAAGCAGTGT+AGG + Chr1:1829147-1829166 None:intergenic 35.0%
CAGCTTATCAGTTGAGATAA+AGG - Chr1:1829396-1829415 MsG0180000126.01.T01:intron 35.0%
CATACATACACTAACTAGTC+AGG - Chr1:1829315-1829334 MsG0180000126.01.T01:intron 35.0%
CTCAAGATATACAGTATCGT+AGG + Chr1:1830401-1830420 None:intergenic 35.0%
CTTCAGATAAGGAAATTGCT+GGG - Chr1:1828476-1828495 MsG0180000126.01.T01:CDS 35.0%
CTTTATCTCAACTGATAAGC+TGG + Chr1:1829398-1829417 None:intergenic 35.0%
GGAAAGCTTGAGAATTGTAA+AGG - Chr1:1830294-1830313 MsG0180000126.01.T01:intron 35.0%
GTTCCAAATATGTTGATGCT+GGG - Chr1:1828536-1828555 MsG0180000126.01.T01:CDS 35.0%
TAAGCTTCGTGTTTCATTTC+AGG - Chr1:1829355-1829374 MsG0180000126.01.T01:intron 35.0%
TATGTCTTCAGATTTCAGAG+CGG - Chr1:1829840-1829859 MsG0180000126.01.T01:intron 35.0%
TCTGAAGAGGAAATAAGTTC+AGG + Chr1:1828463-1828482 None:intergenic 35.0%
TCTTCAGATAAGGAAATTGC+TGG - Chr1:1828475-1828494 MsG0180000126.01.T01:CDS 35.0%
TTAATTCCGTAAGCTCTTCA+GGG - Chr1:1827956-1827975 MsG0180000126.01.T01:intron 35.0%
TTATCTCAACTGATAAGCTG+GGG + Chr1:1829396-1829415 None:intergenic 35.0%
TTCCAAATATGTTGATGCTG+GGG - Chr1:1828537-1828556 MsG0180000126.01.T01:CDS 35.0%
! ACATATTTGGAACCAAGCAA+AGG + Chr1:1828529-1828548 None:intergenic 35.0%
! ATGCAAAAGATGGATTCTGA+TGG + Chr1:1831025-1831044 None:intergenic 35.0%
! CTGACTAGTTAGTGTATGTA+TGG + Chr1:1829317-1829336 None:intergenic 35.0%
! TGAAAGTACAGATGCATCTA+TGG - Chr1:1830006-1830025 MsG0180000126.01.T01:intron 35.0%
! TGTTTCATTTCAGGTAGTCA+AGG - Chr1:1829364-1829383 MsG0180000126.01.T01:intron 35.0%
! TTGGTGATTTGTATTCCTCA+AGG + Chr1:1828697-1828716 None:intergenic 35.0%
!! ATGTTTTAGACCAAGGAAAG+AGG - Chr1:1830360-1830379 MsG0180000126.01.T01:intron 35.0%
!! GAAAGTACCAAAACAAGTAC+AGG + Chr1:1828821-1828840 None:intergenic 35.0%
!! TTTTGGTTAGTAGGTCTTGA+CGG - Chr1:1830526-1830545 MsG0180000126.01.T01:intron 35.0%
!!! TTCATATTTTAACCAACGGG+AGG + Chr1:1829170-1829189 None:intergenic 35.0%
AAGAAACTGCTATCAAGAGG+AGG - Chr1:1829616-1829635 MsG0180000126.01.T01:intron 40.0%
AAGTCTCAGTATGCAACTTC+AGG + Chr1:1830869-1830888 None:intergenic 40.0%
ACTTGAAGATGAACTTCAGC+CGG - Chr1:1830036-1830055 MsG0180000126.01.T01:intron 40.0%
CATTACTTCTCAACAGAGCA+TGG - Chr1:1830207-1830226 MsG0180000126.01.T01:CDS 40.0%
CATTGGGTTTATAGAGGTGA+AGG - Chr1:1827548-1827567 MsG0180000126.01.T01:three_prime_UTR 40.0%
CTGAAGAAACTGCTATCAAG+AGG - Chr1:1829613-1829632 MsG0180000126.01.T01:intron 40.0%
GAGATGAGCAAAATGGTTCA+TGG - Chr1:1830815-1830834 MsG0180000126.01.T01:intron 40.0%
GATCATTCAATCTTCCCTCA+AGG - Chr1:1828970-1828989 MsG0180000126.01.T01:intron 40.0%
GCAAGCACGTTAGATCTATT+TGG - Chr1:1827833-1827852 MsG0180000126.01.T01:CDS 40.0%
GGTTCCAAATATGTTGATGC+TGG - Chr1:1828535-1828554 MsG0180000126.01.T01:CDS 40.0%
GTAGGAAGAAATCCGCATTT+AGG + Chr1:1829579-1829598 None:intergenic 40.0%
GTCTCAAAACCATACCTTGA+GGG + Chr1:1828987-1829006 None:intergenic 40.0%
TACCCCAGCATCAACATATT+TGG + Chr1:1828542-1828561 None:intergenic 40.0%
TGAAGAAGATGCAGCTCATT+GGG - Chr1:1827532-1827551 MsG0180000126.01.T01:three_prime_UTR 40.0%
TGTCTCAAAACCATACCTTG+AGG + Chr1:1828988-1829007 None:intergenic 40.0%
TTCAATCTTCCCTCAAGGTA+TGG - Chr1:1828975-1828994 MsG0180000126.01.T01:intron 40.0%
TTCATTCTGAATGCTGTCTC+CGG - Chr1:1829241-1829260 MsG0180000126.01.T01:intron 40.0%
TTTAGACCAAGGAAAGAGGA+GGG - Chr1:1830364-1830383 MsG0180000126.01.T01:intron 40.0%
! AAGGACTCCTGATTTTTGCA+CGG + Chr1:1830119-1830138 None:intergenic 40.0%
! CTCAGACATTGATCTAGAAC+AGG + Chr1:1828588-1828607 None:intergenic 40.0%
! TCTCTGACCTGTACTTGTTT+TGG - Chr1:1828811-1828830 MsG0180000126.01.T01:intron 40.0%
! TCTGAAATTGCAACATGGAG+AGG - Chr1:1829665-1829684 MsG0180000126.01.T01:CDS 40.0%
! TTTTAGACCAAGGAAAGAGG+AGG - Chr1:1830363-1830382 MsG0180000126.01.T01:intron 40.0%
!! CAAGCTCTGAAATTGCAACA+TGG - Chr1:1829660-1829679 MsG0180000126.01.T01:CDS 40.0%
AACCGTCTCAAGGAATTCCT+TGG + Chr1:1828863-1828882 None:intergenic 45.0%
AAGACAAAGAGGAAGAGGGA+AGG + Chr1:1829512-1829531 None:intergenic 45.0%
AAGCAACCCTGAAGAGCTTA+CGG + Chr1:1827965-1827984 None:intergenic 45.0%
AATGGTTCTCTCATACTCGG+AGG - Chr1:1829968-1829987 MsG0180000126.01.T01:intron 45.0%
ACTGAAGCCGTGCAAAAATC+AGG - Chr1:1830109-1830128 MsG0180000126.01.T01:CDS 45.0%
AGACAAAGAGGAAGAGGGAA+GGG + Chr1:1829511-1829530 None:intergenic 45.0%
AGCCAAAGACAAAGAGGAAG+AGG + Chr1:1829517-1829536 None:intergenic 45.0%
AGTCCTTGATAAGCTCCTTG+AGG - Chr1:1830132-1830151 MsG0180000126.01.T01:CDS 45.0%
ATCACTCTGTGCTTACCTTG+AGG - Chr1:1828679-1828698 MsG0180000126.01.T01:CDS 45.0%
ATCCAGAGAACAGATCGAGT+GGG + Chr1:1829877-1829896 None:intergenic 45.0%
ATGGTTCTCTCATACTCGGA+GGG - Chr1:1829969-1829988 MsG0180000126.01.T01:intron 45.0%
CAGTGATGCAGAATGCAAAG+AGG + Chr1:1830272-1830291 None:intergenic 45.0%
CATGTCATGAAGCCTTTGCT+TGG - Chr1:1828514-1828533 MsG0180000126.01.T01:CDS 45.0%
CATTCTTCTCAACCGTCTCA+AGG + Chr1:1828873-1828892 None:intergenic 45.0%
CCATCATCAGCTTGAATGAC+CGG + Chr1:1830058-1830077 None:intergenic 45.0%
CTCTTTGCATTCTGCATCAC+TGG - Chr1:1830270-1830289 MsG0180000126.01.T01:CDS 45.0%
CTGAAGAAGATGCAGCTCAT+TGG - Chr1:1827531-1827550 MsG0180000126.01.T01:three_prime_UTR 45.0%
GAACCTCAAGGAGCTTATCA+AGG + Chr1:1830138-1830157 None:intergenic 45.0%
GACCAAGGAATTCCTTGAGA+CGG - Chr1:1828858-1828877 MsG0180000126.01.T01:intron 45.0%
GCAGCTCATTGGGTTTATAG+AGG - Chr1:1827542-1827561 MsG0180000126.01.T01:three_prime_UTR 45.0%
GCCAAAGACAAAGAGGAAGA+GGG + Chr1:1829516-1829535 None:intergenic 45.0%
TCCCTCTTCCTCTTTGTCTT+TGG - Chr1:1829512-1829531 MsG0180000126.01.T01:intron 45.0%
TCTCAGTTACTGGGATCATC+AGG + Chr1:1828159-1828178 None:intergenic 45.0%
TGAATTACATTGCCTCCCGT+TGG - Chr1:1829155-1829174 MsG0180000126.01.T01:intron 45.0%
TGTCAAGCTTCTGAACCTCA+AGG + Chr1:1830150-1830169 None:intergenic 45.0%
TTACTGGGATCATCAGGAGA+GGG + Chr1:1828153-1828172 None:intergenic 45.0%
TTTGCATTCTGCATCACTGG+AGG - Chr1:1830273-1830292 MsG0180000126.01.T01:CDS 45.0%
! CTTTTGCAGTTGCTGCTATC+AGG + Chr1:1830324-1830343 None:intergenic 45.0%
!! GACTCACATTTTGGTGACCA+AGG - Chr1:1828843-1828862 MsG0180000126.01.T01:intron 45.0%
!! TGGGCGAGTTGATGTTTCAA+GGG - Chr1:1828912-1828931 MsG0180000126.01.T01:intron 45.0%
AAACATCAACTCGCCCAGCA+GGG + Chr1:1828909-1828928 None:intergenic 50.0%
AACCCACTCGATCTGTTCTC+TGG - Chr1:1829872-1829891 MsG0180000126.01.T01:intron 50.0%
AATCAGCGTTGCAAGGAGTG+AGG - Chr1:1828389-1828408 MsG0180000126.01.T01:CDS 50.0%
AGGAAGGAATCAGCGTTGCA+AGG - Chr1:1828382-1828401 MsG0180000126.01.T01:CDS 50.0%
CATTCGCAAGTCTCCAAGGA+AGG - Chr1:1828366-1828385 MsG0180000126.01.T01:CDS 50.0%
CCGGTCATTCAAGCTGATGA+TGG - Chr1:1830055-1830074 MsG0180000126.01.T01:intron 50.0%
CTACCCTCCTCACATGCTAT+TGG - Chr1:1830969-1830988 MsG0180000126.01.T01:intron 50.0%
GAGCATGGCGCGTAAAGAAT+TGG - Chr1:1830222-1830241 MsG0180000126.01.T01:CDS 50.0%
GATCCAGAGAACAGATCGAG+TGG + Chr1:1829878-1829897 None:intergenic 50.0%
GGACCAATAGCATGTGAGGA+GGG + Chr1:1830975-1830994 None:intergenic 50.0%
GTTACTGGGATCATCAGGAG+AGG + Chr1:1828154-1828173 None:intergenic 50.0%
GTTGGGACCAATAGCATGTG+AGG + Chr1:1830979-1830998 None:intergenic 50.0%
TCGCTCTGACTCCACATGAA+CGG - Chr1:1828419-1828438 MsG0180000126.01.T01:CDS 50.0%
TTCATGTGGAGTCAGAGCGA+TGG + Chr1:1828419-1828438 None:intergenic 50.0%
TTCCAGATGAGCCGTTCATG+TGG + Chr1:1828433-1828452 None:intergenic 50.0%
!! CTGGGCGAGTTGATGTTTCA+AGG - Chr1:1828911-1828930 MsG0180000126.01.T01:intron 50.0%
!! TGCAACGCTGATTCCTTCCT+TGG + Chr1:1828382-1828401 None:intergenic 50.0%
CCAAGGAAAGAGGAGGGTTC+TGG - Chr1:1830370-1830389 MsG0180000126.01.T01:intron 55.0%
CCAGAACCCTCCTCTTTCCT+TGG + Chr1:1830373-1830392 None:intergenic 55.0%
CTCCACATGAACGGCTCATC+TGG - Chr1:1828428-1828447 MsG0180000126.01.T01:CDS 55.0%
GAAACATCAACTCGCCCAGC+AGG + Chr1:1828910-1828929 None:intergenic 55.0%
GGGACCAATAGCATGTGAGG+AGG + Chr1:1830976-1830995 None:intergenic 55.0%
TCTCTCATACTCGGAGGGCT+TGG - Chr1:1829974-1829993 MsG0180000126.01.T01:intron 55.0%
TGCGCATTCGCAAGTCTCCA+AGG - Chr1:1828362-1828381 MsG0180000126.01.T01:CDS 55.0%
TGGGATCATCAGGAGAGGGA+AGG + Chr1:1828149-1828168 None:intergenic 55.0%
TTGCTAGTCAACGCCCTGCT+GGG - Chr1:1828893-1828912 MsG0180000126.01.T01:intron 55.0%
ATACTCGGAGGGCTTGGAGG+TGG - Chr1:1829980-1829999 MsG0180000126.01.T01:intron 60.0%
CTCATACTCGGAGGGCTTGG+AGG - Chr1:1829977-1829996 MsG0180000126.01.T01:intron 60.0%
GTTGCTAGTCAACGCCCTGC+TGG - Chr1:1828892-1828911 MsG0180000126.01.T01:intron 60.0%
Chromosome Type Strat End Strand Name
Chr1 gene 1827482 1831144 1827482 ID=MsG0180000126.01;Name=MsG0180000126.01
Chr1 mRNA 1827482 1831144 1827482 ID=MsG0180000126.01.T01;Parent=MsG0180000126.01;Name=MsG0180000126.01.T01;_AED=0.49;_eAED=0.51;_QI=0|0.6|0.66|1|1|1|6|282|423
Chr1 exon 1831027 1831144 1831027 ID=MsG0180000126.01.T01:exon:6990;Parent=MsG0180000126.01.T01
Chr1 exon 1830068 1830300 1830068 ID=MsG0180000126.01.T01:exon:6991;Parent=MsG0180000126.01.T01
Chr1 exon 1829635 1829782 1829635 ID=MsG0180000126.01.T01:exon:6992;Parent=MsG0180000126.01.T01
Chr1 exon 1829209 1829249 1829209 ID=MsG0180000126.01.T01:exon:6993;Parent=MsG0180000126.01.T01
Chr1 exon 1828179 1828775 1828179 ID=MsG0180000126.01.T01:exon:6994;Parent=MsG0180000126.01.T01
Chr1 exon 1827482 1827898 1827482 ID=MsG0180000126.01.T01:exon:6995;Parent=MsG0180000126.01.T01
Chr1 CDS 1831027 1831144 1831027 ID=MsG0180000126.01.T01:cds;Parent=MsG0180000126.01.T01
Chr1 CDS 1830068 1830300 1830068 ID=MsG0180000126.01.T01:cds;Parent=MsG0180000126.01.T01
Chr1 CDS 1829635 1829782 1829635 ID=MsG0180000126.01.T01:cds;Parent=MsG0180000126.01.T01
Chr1 CDS 1829209 1829249 1829209 ID=MsG0180000126.01.T01:cds;Parent=MsG0180000126.01.T01
Chr1 CDS 1828179 1828775 1828179 ID=MsG0180000126.01.T01:cds;Parent=MsG0180000126.01.T01
Chr1 CDS 1827764 1827898 1827764 ID=MsG0180000126.01.T01:cds;Parent=MsG0180000126.01.T01
Chr1 three_prime_UTR 1827482 1827763 1827482 ID=MsG0180000126.01.T01:three_prime_utr;Parent=MsG0180000126.01.T01
Gene Sequence

>MsG0180000126.01.T01

ATGCCTGACAAAGATTTTCATGTGAAAAAACAGATGGAGTTGACTTTAACTGAAGAAGATGCAGCTCATTGGGTTTATAGAGGTGAAGGTGCTGCTAATATTGTTCTCTCTTACACTGAATATCCAAATGTGTCTTACTTAGATTGGAAAGTGATGCGCATTCGCAAGTCTCCAAGGAAGGAATCAGCGTTGCAAGGAGTGAGGAATACCATCGCTCTGACTCCACATGAACGGCTCATCTGGAAAAATGTACCTGAACTTATTTCCTCTTCAGATAAGGAAATTGCTGGGCAACTATATGTAGAGCATGTCATGAAGCCTTTGCTTGGTTCCAAATATGTTGATGCTGGGACTCACATTTTGGTGACCAAGGAATTCCTTGAGACGGTTGAGAAGAATGTTGCTAGTCAACGCCCTGCTGGGCGAGTTGATGTTTCAAGGGTTGATACACAATGTGATTTTAGTCTTCTCATGTCGGATCATTCAATCTTCCCTCAAGGTAGTCAAGGATCTATCCCCAGCTTATCAGTTGAGATAAAGATTTCAGAGCGGAGTGTGTACAACCCACTCGATCTGTTCTCTGGATCCAAAGAAAGAATTCATAAAGCCATCAAAGATCTCTTTACTACTCCTCAAAACAATTTCCGTGTATTTTTAAATGGTTCTCTCATACTCGGAGGGCTTGGAGGTGGTGCTGAAAGTACAGATGCATCTATGGCTAAAGTACTTGAAGATGAACTTCAGCCGGTCATTCAAGCTGATGATGGCCAATGCACAGAGAACTTATTTACTCTTGTTACTGAAGCCGTGCAAAAATCAGGAGTCCTTGATAAGCTCCTTGAGGTTCAGAAGCTTGACAATATTGACATAGAAGGAGTAATTCATGCATATTACAACATTACTTCTCAACAGAGCATGGCGCGTAAAGAATTGGATGAAGAACAAGCAAAAAAATATGCCTCTTTGCATTCTGCATCACTGGAGGAAAGCTTGAGAATTGTAAAGGACTACCTGATAGCAGCAACTGCAAAAGACTGCAGTTTGATGATATGTTTTAGACCAAGGAAAGAGGAGGGTTCTGGATCTGCCTACGATACTGTATATCTTGAGTCAACTAAGCAAGCCTTTGATTTTAAGGTGCATTTTATTGACCTCGATTTAAAACGGTTGAATAAAGTGGAAGACTACCACAAGTTAGATAAGAAGATAGTGAACTGTTACAAAGAGATGAGCAAAATGGTTCATGGAAGAAATGGAGATGCAAACTTATAG
Protein sequence

>MsG0180000126.01.T01

MPDKDFHVKKQMELTLTEEDAAHWVYRGEGAANIVLSYTEYPNVSYLDWKVMRIRKSPRKESALQGVRNTIALTPHERLIWKNVPELISSSDKEIAGQLYVEHVMKPLLGSKYVDAGTHILVTKEFLETVEKNVASQRPAGRVDVSRVDTQCDFSLLMSDHSIFPQGSQGSIPSLSVEIKISERSVYNPLDLFSGSKERIHKAIKDLFTTPQNNFRVFLNGSLILGGLGGGAESTDASMAKVLEDELQPVIQADDGQCTENLFTLVTEAVQKSGVLDKLLEVQKLDNIDIEGVIHAYYNITSQQSMARKELDEEQAKKYASLHSASLEESLRIVKDYLIAATAKDCSLMICFRPRKEEGSGSAYDTVYLESTKQAFDFKVHFIDLDLKRLNKVEDYHKLDKKIVNCYKEMSKMVHGRNGDANL*