AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180000377.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180000377.01.T01 MTR_1g017020 94.886 176 9 0 28 203 1 176 1.36e-113 344
MsG0180000377.01.T01 MTR_1g017020 30.464 151 103 1 43 193 190 338 7.20e-20 87.4
MsG0180000377.01.T01 MTR_1g017020 28.000 175 100 7 35 193 350 514 1.85e-14 71.6
MsG0180000377.01.T01 MTR_3g103610 33.146 178 111 2 22 193 2 177 1.08e-23 98.6
MsG0180000377.01.T01 MTR_3g103610 25.882 170 110 4 35 193 189 353 7.73e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180000377.01.T01 AT1G58370 45.455 209 98 4 1 193 1 209 1.10e-48 170
MsG0180000377.01.T01 AT1G58370 29.801 151 104 1 43 193 227 375 9.06e-19 84.3
MsG0180000377.01.T01 AT1G58370 25.455 110 77 1 84 193 448 552 4.33e-11 62.0
MsG0180000377.01.T01 AT1G58370 51.220 164 79 1 31 193 18 181 1.55e-47 167
MsG0180000377.01.T01 AT1G58370 29.801 151 104 1 43 193 199 347 1.00e-18 84.3
MsG0180000377.01.T01 AT1G58370 25.455 110 77 1 84 193 420 524 4.92e-11 61.6
MsG0180000377.01.T01 AT1G10050 53.374 163 62 2 40 199 181 332 2.31e-45 160
MsG0180000377.01.T01 AT1G10050 53.378 148 68 1 42 188 5 152 7.06e-42 150
MsG0180000377.01.T01 AT1G10050 37.179 156 91 3 33 188 342 490 1.81e-21 92.0
MsG0180000377.01.T01 AT1G10050 30.357 112 72 2 83 193 568 674 1.72e-12 66.2
MsG0180000377.01.T01 AT1G10050 53.374 163 62 2 40 199 181 332 2.31e-45 160
MsG0180000377.01.T01 AT1G10050 53.378 148 68 1 42 188 5 152 7.06e-42 150
MsG0180000377.01.T01 AT1G10050 37.179 156 91 3 33 188 342 490 1.81e-21 92.0
MsG0180000377.01.T01 AT1G10050 30.357 112 72 2 83 193 568 674 1.72e-12 66.2
MsG0180000377.01.T01 AT1G10050 53.374 163 62 2 40 199 213 364 2.64e-45 160
MsG0180000377.01.T01 AT1G10050 53.378 148 68 1 42 188 37 184 7.67e-42 150
MsG0180000377.01.T01 AT1G10050 37.179 156 91 3 33 188 374 522 2.00e-21 92.0
MsG0180000377.01.T01 AT1G10050 30.357 112 72 2 83 193 600 706 2.09e-12 65.9
MsG0180000377.01.T01 AT4G08160 31.788 151 101 1 43 193 27 175 8.01e-21 90.1
MsG0180000377.01.T01 AT4G08160 35.345 116 65 3 83 193 247 357 3.66e-15 73.9
MsG0180000377.01.T01 AT4G08160 31.788 151 101 1 43 193 27 175 1.12e-20 89.7
MsG0180000377.01.T01 AT4G08160 35.345 116 65 3 83 193 247 357 4.93e-15 73.6

Find 46 sgRNAs with CRISPR-Local

Find 82 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGTTCTGCTTACACGGTTT+CGG 0.290637 1:-5168261 MsG0180000377.01.T01:CDS
GCTACAAATTATTTGTTCAT+TGG 0.326978 1:-5168161 MsG0180000377.01.T01:CDS
GTATGATATACGTTGTCATT+TGG 0.365712 1:+5167903 None:intergenic
AGGTGTAAATTTGATGGATT+CGG 0.376961 1:-5168363 MsG0180000377.01.T01:CDS
CTACAGAACTTCTGTTATGA+AGG 0.391792 1:-5168054 MsG0180000377.01.T01:intron
TGATCAAGGTGTAAATTTGA+TGG 0.401599 1:-5168369 MsG0180000377.01.T01:CDS
TACACCTTGATCACCTGCTT+TGG 0.402831 1:+5168379 None:intergenic
CTTCTGTTATGAAGGGTAGC+TGG 0.404922 1:-5168046 MsG0180000377.01.T01:CDS
GAAGGGTAGCTGGGAAAAGT+TGG 0.414542 1:-5168036 MsG0180000377.01.T01:CDS
GACCTTGCCAGCATTCGTTT+CGG 0.423092 1:+5168315 None:intergenic
AATTGAGATGCCAAGAGTTC+AGG 0.433547 1:+5168426 None:intergenic
TGGAGTATCCGGCCTTTCTC+AGG 0.437664 1:-5168231 MsG0180000377.01.T01:CDS
CTTATAAGCAAGTCAATTCC+AGG 0.441038 1:+5167951 None:intergenic
AAATAAAATACAACCCGATC+GGG 0.455406 1:+5167987 None:intergenic
TATTTGGAAGGACCTGCTCC+TGG 0.458649 1:-5167969 MsG0180000377.01.T01:CDS
ACAAATTATTTGTTCATTGG+AGG 0.463668 1:-5168158 MsG0180000377.01.T01:CDS
TCTCAAATGACCTGAACTCT+TGG 0.471487 1:-5168436 MsG0180000377.01.T01:CDS
CTGCTTTGGACGAAATTACA+TGG 0.475645 1:+5168393 None:intergenic
TTCTGTTATGAAGGGTAGCT+GGG 0.476279 1:-5168045 MsG0180000377.01.T01:CDS
TGGCATCTCAATTGCTGCAA+TGG 0.476409 1:-5168416 MsG0180000377.01.T01:CDS
AGCAAGTCAATTCCAGGAGC+AGG 0.502349 1:+5167957 None:intergenic
TTCCATTGGTTCTGCTTACA+CGG 0.506731 1:-5168267 MsG0180000377.01.T01:CDS
TTTGTTTGACAGATAATGGC+AGG 0.509365 1:-5168515 MsG0180000377.01.T01:intron
GAAATCTCACTCTCACTCTC+AGG 0.509622 1:-5169114 MsG0180000377.01.T01:intron
CAAATTATTTGTTCATTGGA+GGG 0.512221 1:-5168157 MsG0180000377.01.T01:intron
TCACCGACCGAAACGAATGC+TGG 0.517919 1:-5168322 MsG0180000377.01.T01:CDS
TTAGCAGCATTGCTCCCACT+TGG 0.518845 1:+5168479 None:intergenic
ATATACGTTGTCATTTGGAA+TGG 0.520996 1:+5167908 None:intergenic
AGCAGATTCCTGAGAAAGGC+CGG 0.525243 1:+5168223 None:intergenic
CACTGTCGACTAAACCCGAT+CGG 0.527900 1:-5168001 MsG0180000377.01.T01:CDS
TCGTCCAAAGCAGGTGATCA+AGG 0.532341 1:-5168383 MsG0180000377.01.T01:CDS
CAAATAAAATACAACCCGAT+CGG 0.533364 1:+5167986 None:intergenic
CATGTAATTTCGTCCAAAGC+AGG 0.534471 1:-5168392 MsG0180000377.01.T01:CDS
CATCAGCAGATTCCTGAGAA+AGG 0.537612 1:+5168219 None:intergenic
ATAATGGCAGGAAACATCTC+TGG 0.543211 1:-5168503 MsG0180000377.01.T01:CDS
ACTGTCGACTAAACCCGATC+GGG 0.543731 1:-5168000 MsG0180000377.01.T01:CDS
ATCACAGACAGAATTTCCAT+TGG 0.553945 1:-5168281 MsG0180000377.01.T01:CDS
GGAAACATCTCTGGTCCAAG+TGG 0.555841 1:-5168494 MsG0180000377.01.T01:CDS
GAGATTTCTTATGTTTCGAG+TGG 0.565861 1:+5169129 None:intergenic
AGATTTCTTATGTTTCGAGT+GGG 0.566960 1:+5169130 None:intergenic
TACAGAACTTCTGTTATGAA+GGG 0.577471 1:-5168053 MsG0180000377.01.T01:intron
GACCGAAACGAATGCTGGCA+AGG 0.591612 1:-5168317 MsG0180000377.01.T01:CDS
AACCGTGTAAGCAGAACCAA+TGG 0.602847 1:+5168265 None:intergenic
TTGCCAGCATTCGTTTCGGT+CGG 0.605263 1:+5168319 None:intergenic
GAAACATCTCTGGTCCAAGT+GGG 0.659237 1:-5168493 MsG0180000377.01.T01:CDS
GGTAGCTGGGAAAAGTTGGA+AGG 0.662560 1:-5168032 MsG0180000377.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCATTTTTTCAAATTATTAC+CGG + Chr1:5168401-5168420 None:intergenic 15.0%
!! AGAGAATCTTAAAAAATAAC+TGG + Chr1:5168001-5168020 None:intergenic 20.0%
!! GAGAATCTTAAAAAATAACT+GGG + Chr1:5168000-5168019 None:intergenic 20.0%
!!! AAATTACAAAGTCTGAAATA+TGG + Chr1:5168091-5168110 None:intergenic 20.0%
!!! TCTATCTTTTAAAGAAATTG+AGG - Chr1:5168962-5168981 MsG0180000377.01.T01:intron 20.0%
! AAAATAATGGTGAACATGTA+AGG + Chr1:5168995-5169014 None:intergenic 25.0%
! AAATAATGGTGAACATGTAA+GGG + Chr1:5168994-5169013 None:intergenic 25.0%
! ACAAATTATTTGTTCATTGG+AGG - Chr1:5168911-5168930 MsG0180000377.01.T01:intron 25.0%
! AGAAGTTCTGTAGAAAATAA+TGG + Chr1:5169008-5169027 None:intergenic 25.0%
! ATCTTAAAAAATAACTGGGA+AGG + Chr1:5167996-5168015 None:intergenic 25.0%
! ATGTGATGTATAATGCTTAA+TGG - Chr1:5168267-5168286 MsG0180000377.01.T01:CDS 25.0%
! CAAATTATTTGTTCATTGGA+GGG - Chr1:5168912-5168931 MsG0180000377.01.T01:intron 25.0%
! GCTACAAATTATTTGTTCAT+TGG - Chr1:5168908-5168927 MsG0180000377.01.T01:intron 25.0%
! TAAGGAAAATAGTTTCTTCT+TGG - Chr1:5168502-5168521 MsG0180000377.01.T01:CDS 25.0%
! TCTTAAAAAATAACTGGGAA+GGG + Chr1:5167995-5168014 None:intergenic 25.0%
!!! TTGTTTTGTTTGACAGATAA+TGG - Chr1:5168550-5168569 MsG0180000377.01.T01:intron 25.0%
AAAAAATGAAAGCGACTGAA+TGG - Chr1:5168412-5168431 MsG0180000377.01.T01:CDS 30.0%
AAATAAAATACAACCCGATC+GGG + Chr1:5169085-5169104 None:intergenic 30.0%
CAAATAAAATACAACCCGAT+CGG + Chr1:5169086-5169105 None:intergenic 30.0%
GTATGATATACGTTGTCATT+TGG + Chr1:5169169-5169188 None:intergenic 30.0%
TACAGAACTTCTGTTATGAA+GGG - Chr1:5169016-5169035 MsG0180000377.01.T01:intron 30.0%
TAGTGCTTCATAGATTAACA+CGG + Chr1:5168344-5168363 None:intergenic 30.0%
TATGGATCATCCAATTTGAT+CGG + Chr1:5168073-5168092 None:intergenic 30.0%
TGATCAAGGTGTAAATTTGA+TGG - Chr1:5168700-5168719 MsG0180000377.01.T01:intron 30.0%
! AGATTTCTTATGTTTCGAGT+GGG + Chr1:5167942-5167961 None:intergenic 30.0%
! ATATACGTTGTCATTTGGAA+TGG + Chr1:5169164-5169183 None:intergenic 30.0%
!! AGGTGTAAATTTGATGGATT+CGG - Chr1:5168706-5168725 MsG0180000377.01.T01:intron 30.0%
!! TACAGTGAAGCTTTAAAAAC+AGG + Chr1:5168216-5168235 None:intergenic 30.0%
AACACAGATATAGATGAGAC+TGG - Chr1:5168363-5168382 MsG0180000377.01.T01:CDS 35.0%
ACACACACACTAAACACTTA+AGG - Chr1:5168484-5168503 MsG0180000377.01.T01:CDS 35.0%
ACACAGATATAGATGAGACT+GGG - Chr1:5168364-5168383 MsG0180000377.01.T01:CDS 35.0%
ATCACAGACAGAATTTCCAT+TGG - Chr1:5168788-5168807 MsG0180000377.01.T01:intron 35.0%
CTACAGAACTTCTGTTATGA+AGG - Chr1:5169015-5169034 MsG0180000377.01.T01:intron 35.0%
CTTATAAGCAAGTCAATTCC+AGG + Chr1:5169121-5169140 None:intergenic 35.0%
GAGATTTCTTATGTTTCGAG+TGG + Chr1:5167943-5167962 None:intergenic 35.0%
GCATCAACACTTCATATTGA+AGG + Chr1:5168450-5168469 None:intergenic 35.0%
! CTTTAAAAACAGGTCTGCAA+CGG + Chr1:5168206-5168225 None:intergenic 35.0%
! TTTGTTTGACAGATAATGGC+AGG - Chr1:5168554-5168573 MsG0180000377.01.T01:intron 35.0%
!! CGATCGGGTTGTATTTTATT+TGG - Chr1:5169084-5169103 MsG0180000377.01.T01:intron 35.0%
!! CGGGTTGTATTTTATTTGGA+AGG - Chr1:5169088-5169107 MsG0180000377.01.T01:intron 35.0%
AATTGAGATGCCAAGAGTTC+AGG + Chr1:5168646-5168665 None:intergenic 40.0%
ATAATGGCAGGAAACATCTC+TGG - Chr1:5168566-5168585 MsG0180000377.01.T01:intron 40.0%
CATGTAATTTCGTCCAAAGC+AGG - Chr1:5168677-5168696 MsG0180000377.01.T01:intron 40.0%
GATCATCCAATTTGATCGGA+CGG + Chr1:5168069-5168088 None:intergenic 40.0%
TAGACCGTCCAATCTTTGAT+AGG - Chr1:5168135-5168154 MsG0180000377.01.T01:intron 40.0%
TCTCAAATGACCTGAACTCT+TGG - Chr1:5168633-5168652 MsG0180000377.01.T01:intron 40.0%
TTCTGTTATGAAGGGTAGCT+GGG - Chr1:5169024-5169043 MsG0180000377.01.T01:intron 40.0%
! CTGCTTTGGACGAAATTACA+TGG + Chr1:5168679-5168698 None:intergenic 40.0%
! CTTCATATTGAAGGCGTGTT+CGG + Chr1:5168441-5168460 None:intergenic 40.0%
!! TTCCATTGGTTCTGCTTACA+CGG - Chr1:5168802-5168821 MsG0180000377.01.T01:intron 40.0%
AACCGTGTAAGCAGAACCAA+TGG + Chr1:5168807-5168826 None:intergenic 45.0%
CATCAGCAGATTCCTGAGAA+AGG + Chr1:5168853-5168872 None:intergenic 45.0%
CCGTCCAATCTTTGATAGGA+CGG - Chr1:5168139-5168158 MsG0180000377.01.T01:intron 45.0%
CCGTCCTATCAAAGATTGGA+CGG + Chr1:5168142-5168161 None:intergenic 45.0%
CTTCTGTTATGAAGGGTAGC+TGG - Chr1:5169023-5169042 MsG0180000377.01.T01:intron 45.0%
GAAACATCTCTGGTCCAAGT+GGG - Chr1:5168576-5168595 MsG0180000377.01.T01:intron 45.0%
GAAATCTCACTCTCACTCTC+AGG - Chr1:5167955-5167974 MsG0180000377.01.T01:CDS 45.0%
GTGACCGTCCTATCAAAGAT+TGG + Chr1:5168146-5168165 None:intergenic 45.0%
TACACCTTGATCACCTGCTT+TGG + Chr1:5168693-5168712 None:intergenic 45.0%
TGGCATCTCAATTGCTGCAA+TGG - Chr1:5168653-5168672 MsG0180000377.01.T01:intron 45.0%
! TACACGGTTTCGGCTTTTGT+TGG - Chr1:5168818-5168837 MsG0180000377.01.T01:intron 45.0%
! TGGTTCTGCTTACACGGTTT+CGG - Chr1:5168808-5168827 MsG0180000377.01.T01:intron 45.0%
!! TCGGCTTTTGTTGGAGTATC+CGG - Chr1:5168827-5168846 MsG0180000377.01.T01:intron 45.0%
!!! TTGTAATTTTAATTTTTTAA+AGG - Chr1:5168102-5168121 MsG0180000377.01.T01:intron 5.0%
AGCAAGTCAATTCCAGGAGC+AGG + Chr1:5169115-5169134 None:intergenic 50.0%
AGCAGATTCCTGAGAAAGGC+CGG + Chr1:5168849-5168868 None:intergenic 50.0%
GAAGGGTAGCTGGGAAAAGT+TGG - Chr1:5169033-5169052 MsG0180000377.01.T01:intron 50.0%
GACCTTGCCAGCATTCGTTT+CGG + Chr1:5168757-5168776 None:intergenic 50.0%
GGAAACATCTCTGGTCCAAG+TGG - Chr1:5168575-5168594 MsG0180000377.01.T01:intron 50.0%
GGTAGCTGGGAAAAGTTGGA+AGG - Chr1:5169037-5169056 MsG0180000377.01.T01:intron 50.0%
GGTCTGCAACGGCTACATTA+AGG + Chr1:5168195-5168214 None:intergenic 50.0%
TATTTGGAAGGACCTGCTCC+TGG - Chr1:5169100-5169119 MsG0180000377.01.T01:intron 50.0%
TCGTCCAAAGCAGGTGATCA+AGG - Chr1:5168686-5168705 MsG0180000377.01.T01:intron 50.0%
TTAGCAGCATTGCTCCCACT+TGG + Chr1:5168593-5168612 None:intergenic 50.0%
TTGCCAGCATTCGTTTCGGT+CGG + Chr1:5168753-5168772 None:intergenic 50.0%
! ACTGTCGACTAAACCCGATC+GGG - Chr1:5169069-5169088 MsG0180000377.01.T01:intron 50.0%
! CACTGTCGACTAAACCCGAT+CGG - Chr1:5169068-5169087 MsG0180000377.01.T01:intron 50.0%
GACCGAAACGAATGCTGGCA+AGG - Chr1:5168752-5168771 MsG0180000377.01.T01:intron 55.0%
GCGTGACCGTCCGATCAAAT+TGG - Chr1:5168060-5168079 MsG0180000377.01.T01:intron 55.0%
TCACCGACCGAAACGAATGC+TGG - Chr1:5168747-5168766 MsG0180000377.01.T01:intron 55.0%
! TGGAGTATCCGGCCTTTCTC+AGG - Chr1:5168838-5168857 MsG0180000377.01.T01:intron 55.0%
AGACTGGGCACGACTCACAC+CGG - Chr1:5168379-5168398 MsG0180000377.01.T01:CDS 60.0%
Chromosome Type Strat End Strand Name
Chr1 gene 5167899 5169192 5167899 ID=MsG0180000377.01;Name=MsG0180000377.01
Chr1 mRNA 5167899 5169192 5167899 ID=MsG0180000377.01.T01;Parent=MsG0180000377.01;Name=MsG0180000377.01.T01;_AED=0.44;_eAED=0.46;_QI=0|0|0|1|0.5|0.66|3|0|205
Chr1 exon 5169115 5169192 5169115 ID=MsG0180000377.01.T01:exon:2790;Parent=MsG0180000377.01.T01
Chr1 exon 5168158 5168525 5168158 ID=MsG0180000377.01.T01:exon:2791;Parent=MsG0180000377.01.T01
Chr1 exon 5167899 5168070 5167899 ID=MsG0180000377.01.T01:exon:2792;Parent=MsG0180000377.01.T01
Chr1 CDS 5169115 5169192 5169115 ID=MsG0180000377.01.T01:cds;Parent=MsG0180000377.01.T01
Chr1 CDS 5168158 5168525 5168158 ID=MsG0180000377.01.T01:cds;Parent=MsG0180000377.01.T01
Chr1 CDS 5167899 5168070 5167899 ID=MsG0180000377.01.T01:cds;Parent=MsG0180000377.01.T01
Gene Sequence

>MsG0180000377.01.T01

ATGAAGAGATTCTTCACAAGCTGTGTCTCAAAGTTTCACTCCCACTCGAAACATAAGAAATCTCACTCTCACTCTCAGATAATGGCAGGAAACATCTCTGGTCCAAGTGGGAGCAATGCTGCTAATATTTTGCTGAATCATGACTTCTCAAATGACCTGAACTCTTGGCATCTCAATTGCTGCAATGGCCATGTAATTTCGTCCAAAGCAGGTGATCAAGGTGTAAATTTGATGGATTCGGATTGTAATTATGCTGTTATCACCGACCGAAACGAATGCTGGCAAGGTCTTGAGCAAGATATCACAGACAGAATTTCCATTGGTTCTGCTTACACGGTTTCGGCTTTTGTTGGAGTATCCGGCCTTTCTCAGGAATCTGCTGATGTGAAAGCTACACTGAAACTAGAATATCATGATTCAGCTACAAATTATTTGTTCATTGGAGGAACTTCTGTTATGAAGGGTAGCTGGGAAAAGTTGGAAGGAACATTTTCACTGTCGACTAAACCCGATCGGGTTGTATTTTATTTGGAAGGACCTGCTCCTGGAATTGACTTGCTTATAAGATCAGTAGAGATCAACTGTTCCATTCCAAATGACAACGTATATCATACATGA

Protein sequence

>MsG0180000377.01.T01

MKRFFTSCVSKFHSHSKHKKSHSHSQIMAGNISGPSGSNAANILLNHDFSNDLNSWHLNCCNGHVISSKAGDQGVNLMDSDCNYAVITDRNECWQGLEQDITDRISIGSAYTVSAFVGVSGLSQESADVKATLKLEYHDSATNYLFIGGTSVMKGSWEKLEGTFSLSTKPDRVVFYLEGPAPGIDLLIRSVEINCSIPNDNVYHT*