Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180000654.01.T01 | KEH39810.1 | 78.829 | 222 | 44 | 1 | 1 | 219 | 9 | 230 | 4.02E-115 | 352 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180000654.01.T01 | Q9LMN7 | 34 | 200 | 108 | 9 | 6 | 199 | 5 | 186 | 8.18E-17 | 82 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180000654.01.T01 | A0A072VNU8 | 78.829 | 222 | 44 | 1 | 1 | 219 | 9 | 230 | 1.92e-115 | 352 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180000653.01 | MsG0180000654.01 | 0.838239 | 3.235303e-57 | 4.306648e-54 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180000654.01.T01 | MTR_1g010250 | 78.829 | 222 | 44 | 1 | 1 | 219 | 9 | 230 | 4.86e-119 | 352 |
| MsG0180000654.01.T01 | MTR_1g010220 | 75.610 | 205 | 44 | 2 | 1 | 203 | 5 | 205 | 2.07e-102 | 311 |
| MsG0180000654.01.T01 | MTR_1g027580 | 52.000 | 200 | 81 | 6 | 7 | 202 | 13 | 201 | 2.29e-56 | 191 |
| MsG0180000654.01.T01 | MTR_1g027660 | 50.777 | 193 | 82 | 5 | 12 | 202 | 2 | 183 | 9.94e-54 | 183 |
| MsG0180000654.01.T01 | MTR_1g027570 | 49.020 | 204 | 91 | 5 | 1 | 202 | 9 | 201 | 1.20e-52 | 181 |
| MsG0180000654.01.T01 | MTR_1g027600 | 46.429 | 196 | 82 | 6 | 5 | 192 | 14 | 194 | 5.26e-43 | 154 |
| MsG0180000654.01.T01 | MTR_1g027640 | 44.792 | 192 | 93 | 6 | 5 | 192 | 14 | 196 | 2.60e-42 | 152 |
| MsG0180000654.01.T01 | MTR_1g027490 | 48.387 | 186 | 76 | 6 | 25 | 203 | 15 | 187 | 3.35e-42 | 151 |
| MsG0180000654.01.T01 | MTR_1g010230 | 42.697 | 178 | 85 | 9 | 25 | 199 | 15 | 178 | 1.28e-33 | 121 |
| MsG0180000654.01.T01 | MTR_1g010260 | 41.538 | 195 | 92 | 12 | 5 | 195 | 12 | 188 | 4.59e-33 | 125 |
| MsG0180000654.01.T01 | MTR_1g010240 | 40.351 | 171 | 52 | 8 | 31 | 195 | 39 | 165 | 8.40e-23 | 96.3 |
| MsG0180000654.01.T01 | MTR_3g088780 | 33.333 | 180 | 107 | 6 | 7 | 182 | 12 | 182 | 5.41e-22 | 94.0 |
| MsG0180000654.01.T01 | MTR_2g009670 | 32.984 | 191 | 100 | 9 | 5 | 182 | 11 | 186 | 5.04e-20 | 88.2 |
| MsG0180000654.01.T01 | MTR_3g088775 | 30.435 | 184 | 107 | 8 | 25 | 201 | 50 | 219 | 6.68e-18 | 82.0 |
| MsG0180000654.01.T01 | MTR_3g088755 | 31.383 | 188 | 107 | 8 | 5 | 184 | 8 | 181 | 1.18e-16 | 78.6 |
| MsG0180000654.01.T01 | MTR_3g088750 | 31.579 | 190 | 102 | 8 | 5 | 182 | 8 | 181 | 1.71e-16 | 78.2 |
| MsG0180000654.01.T01 | MTR_3g088760 | 31.443 | 194 | 106 | 10 | 1 | 183 | 1 | 178 | 2.21e-16 | 77.8 |
| MsG0180000654.01.T01 | MTR_2g031520 | 29.050 | 179 | 101 | 8 | 27 | 193 | 36 | 200 | 8.08e-11 | 61.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180000654.01.T01 | AT1G21230 | 34.000 | 200 | 108 | 9 | 6 | 199 | 5 | 186 | 8.34e-18 | 82.0 |
| MsG0180000654.01.T01 | AT1G21250 | 30.457 | 197 | 113 | 9 | 1 | 191 | 1 | 179 | 4.76e-15 | 73.9 |
| MsG0180000654.01.T01 | AT1G21270 | 30.151 | 199 | 117 | 9 | 1 | 195 | 1 | 181 | 5.43e-15 | 73.9 |
| MsG0180000654.01.T01 | AT1G21210 | 27.660 | 188 | 119 | 7 | 14 | 199 | 12 | 184 | 1.95e-14 | 72.0 |
| MsG0180000654.01.T01 | AT1G21240 | 34.682 | 173 | 91 | 8 | 11 | 177 | 10 | 166 | 2.96e-14 | 71.6 |
Find 41 sgRNAs with CRISPR-Local
Find 45 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTGATCCTAAAATGGTAAT+TGG | 0.134187 | 1:-9264996 | MsG0180000654.01.T01:CDS |
| TACACAAGTGAGATAAAATT+TGG | 0.171644 | 1:+9265295 | None:intergenic |
| ATGTTCTACAGAAAATTTAT+AGG | 0.278643 | 1:+9264822 | None:intergenic |
| AAAAGGTGTATTCGCTTCTT+TGG | 0.280160 | 1:+9265154 | None:intergenic |
| ACTGGAAAATGCACAGGATT+AGG | 0.293882 | 1:-9264966 | MsG0180000654.01.T01:CDS |
| CCCAGCTTCTACACTGTCTA+TGG | 0.303284 | 1:-9264883 | MsG0180000654.01.T01:CDS |
| TTTCCTATGCCAAATGGATA+TGG | 0.307866 | 1:+9265356 | None:intergenic |
| TTTCATTTCCATTTGACCTT+GGG | 0.353338 | 1:+9265220 | None:intergenic |
| TTGATCCTAAAATGGTAATT+GGG | 0.383670 | 1:-9264995 | MsG0180000654.01.T01:CDS |
| CTGGAAAATGCACAGGATTA+GGG | 0.399616 | 1:-9264965 | MsG0180000654.01.T01:CDS |
| AAGGGTGCTACTTATTCAAC+TGG | 0.409648 | 1:-9265041 | MsG0180000654.01.T01:CDS |
| CATAAATCCCCAAGGTCAAA+TGG | 0.428208 | 1:-9265228 | MsG0180000654.01.T01:CDS |
| TGATCATATGCAAATGCAAA+TGG | 0.432367 | 1:+9265431 | None:intergenic |
| TGGGAATTTAAATGCTTGTA+TGG | 0.444765 | 1:+9264902 | None:intergenic |
| GTAAAAGTTCCATATCCATT+TGG | 0.455265 | 1:-9265365 | MsG0180000654.01.T01:CDS |
| ATTTCATTTCCATTTGACCT+TGG | 0.455821 | 1:+9265219 | None:intergenic |
| GACCTTGGGGATTTATGTCT+AGG | 0.457793 | 1:+9265234 | None:intergenic |
| GTTGAATTTCCTATGCCAAA+TGG | 0.483252 | 1:+9265350 | None:intergenic |
| GAAAACAAATTCATTACCGT+TGG | 0.488115 | 1:-9265098 | MsG0180000654.01.T01:CDS |
| TCCATAGACAGTGTAGAAGC+TGG | 0.493012 | 1:+9264882 | None:intergenic |
| ATATCTCAACAGTAATTTCA+AGG | 0.503655 | 1:-9265060 | MsG0180000654.01.T01:CDS |
| TTCATTTCCATTTGACCTTG+GGG | 0.514591 | 1:+9265221 | None:intergenic |
| ACCGTTGGTTGCGACAGTTA+CGG | 0.525678 | 1:-9265083 | MsG0180000654.01.T01:CDS |
| TTCACTCACAATTTCAAGCA+CGG | 0.533737 | 1:-9265120 | MsG0180000654.01.T01:CDS |
| GTTCCATATCCATTTGGCAT+AGG | 0.546347 | 1:-9265359 | MsG0180000654.01.T01:CDS |
| ATGGTAATTGGGAATAACAC+TGG | 0.558794 | 1:-9264984 | MsG0180000654.01.T01:CDS |
| TATTCCTTTATTGCAAAACA+AGG | 0.561782 | 1:-9264847 | MsG0180000654.01.T01:CDS |
| CAACCTCAACAAGTTCTACC+TGG | 0.568143 | 1:-9265410 | MsG0180000654.01.T01:CDS |
| CCATAGACAGTGTAGAAGCT+GGG | 0.575477 | 1:+9264883 | None:intergenic |
| TCCGTAACTGTCGCAACCAA+CGG | 0.580306 | 1:+9265082 | None:intergenic |
| CCTGGCTGCAATTACACTTG+TGG | 0.581610 | 1:-9265392 | MsG0180000654.01.T01:CDS |
| CAGCCAGGTAGAACTTGTTG+AGG | 0.582281 | 1:+9265407 | None:intergenic |
| CCACAAGTGTAATTGCAGCC+AGG | 0.589374 | 1:+9265392 | None:intergenic |
| TATCTCAACAGTAATTTCAA+GGG | 0.592511 | 1:-9265059 | MsG0180000654.01.T01:CDS |
| AATAACACTGGAAAATGCAC+AGG | 0.601342 | 1:-9264972 | MsG0180000654.01.T01:CDS |
| AATTTGGAATTCATGTAACA+TGG | 0.607566 | 1:+9265311 | None:intergenic |
| AGTGAAGGAACTCTGAGAAA+AGG | 0.613235 | 1:+9265137 | None:intergenic |
| GTGCTTGAAATTGTGAGTGA+AGG | 0.642605 | 1:+9265122 | None:intergenic |
| GGCTGCAATTACACTTGTGG+AGG | 0.656932 | 1:-9265389 | MsG0180000654.01.T01:CDS |
| GTCCTAGACATAAATCCCCA+AGG | 0.665685 | 1:-9265236 | MsG0180000654.01.T01:CDS |
| AACAACTCAAAACTAATACG+TGG | 0.715483 | 1:-9265272 | MsG0180000654.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTATGATTTTGATCCTAAAA+TGG | - | Chr1:9265291-9265310 | MsG0180000654.01.T01:CDS | 20.0% |
| ! | ATATCTCAACAGTAATTTCA+AGG | - | Chr1:9265234-9265253 | MsG0180000654.01.T01:CDS | 25.0% |
| ! | TACACAAGTGAGATAAAATT+TGG | + | Chr1:9265002-9265021 | None:intergenic | 25.0% |
| ! | TATCTCAACAGTAATTTCAA+GGG | - | Chr1:9265235-9265254 | MsG0180000654.01.T01:CDS | 25.0% |
| ! | TATTCCTTTATTGCAAAACA+AGG | - | Chr1:9265447-9265466 | MsG0180000654.01.T01:CDS | 25.0% |
| ! | TTGATCCTAAAATGGTAATT+GGG | - | Chr1:9265299-9265318 | MsG0180000654.01.T01:CDS | 25.0% |
| ! | TTTGATCCTAAAATGGTAAT+TGG | - | Chr1:9265298-9265317 | MsG0180000654.01.T01:CDS | 25.0% |
| !! | AATTTGGAATTCATGTAACA+TGG | + | Chr1:9264986-9265005 | None:intergenic | 25.0% |
| !! | ATGGTGATATTTTTCATAAG+TGG | + | Chr1:9265376-9265395 | None:intergenic | 25.0% |
| !! | TTATTCCCAATTACCATTTT+AGG | + | Chr1:9265307-9265326 | None:intergenic | 25.0% |
| AACAACTCAAAACTAATACG+TGG | - | Chr1:9265022-9265041 | MsG0180000654.01.T01:CDS | 30.0% | |
| ATTTCATTTCCATTTGACCT+TGG | + | Chr1:9265078-9265097 | None:intergenic | 30.0% | |
| GAAAACAAATTCATTACCGT+TGG | - | Chr1:9265196-9265215 | MsG0180000654.01.T01:CDS | 30.0% | |
| GTAAAAGTTCCATATCCATT+TGG | - | Chr1:9264929-9264948 | MsG0180000654.01.T01:CDS | 30.0% | |
| TGATCATATGCAAATGCAAA+TGG | + | Chr1:9264866-9264885 | None:intergenic | 30.0% | |
| TGGGAATTTAAATGCTTGTA+TGG | + | Chr1:9265395-9265414 | None:intergenic | 30.0% | |
| TTTCATTTCCATTTGACCTT+GGG | + | Chr1:9265077-9265096 | None:intergenic | 30.0% | |
| ! | GTGATATTTTTCATAAGTGG+AGG | + | Chr1:9265373-9265392 | None:intergenic | 30.0% |
| AATAACACTGGAAAATGCAC+AGG | - | Chr1:9265322-9265341 | MsG0180000654.01.T01:CDS | 35.0% | |
| ATGGTAATTGGGAATAACAC+TGG | - | Chr1:9265310-9265329 | MsG0180000654.01.T01:CDS | 35.0% | |
| GTTGAATTTCCTATGCCAAA+TGG | + | Chr1:9264947-9264966 | None:intergenic | 35.0% | |
| TTCACTCACAATTTCAAGCA+CGG | - | Chr1:9265174-9265193 | MsG0180000654.01.T01:CDS | 35.0% | |
| TTCATTTCCATTTGACCTTG+GGG | + | Chr1:9265076-9265095 | None:intergenic | 35.0% | |
| TTTCCTATGCCAAATGGATA+TGG | + | Chr1:9264941-9264960 | None:intergenic | 35.0% | |
| ! | AAAAGGTGTATTCGCTTCTT+TGG | + | Chr1:9265143-9265162 | None:intergenic | 35.0% |
| !!! | GGATCCTTGTTTTGCAATAA+AGG | + | Chr1:9265454-9265473 | None:intergenic | 35.0% |
| ACTGGAAAATGCACAGGATT+AGG | - | Chr1:9265328-9265347 | MsG0180000654.01.T01:CDS | 40.0% | |
| CATAAATCCCCAAGGTCAAA+TGG | - | Chr1:9265066-9265085 | MsG0180000654.01.T01:CDS | 40.0% | |
| CTGGAAAATGCACAGGATTA+GGG | - | Chr1:9265329-9265348 | MsG0180000654.01.T01:CDS | 40.0% | |
| GTGCTTGAAATTGTGAGTGA+AGG | + | Chr1:9265175-9265194 | None:intergenic | 40.0% | |
| GTTCCATATCCATTTGGCAT+AGG | - | Chr1:9264935-9264954 | MsG0180000654.01.T01:CDS | 40.0% | |
| ! | AGTGAAGGAACTCTGAGAAA+AGG | + | Chr1:9265160-9265179 | None:intergenic | 40.0% |
| !! | AAGGGTGCTACTTATTCAAC+TGG | - | Chr1:9265253-9265272 | MsG0180000654.01.T01:CDS | 40.0% |
| CAACCTCAACAAGTTCTACC+TGG | - | Chr1:9264884-9264903 | MsG0180000654.01.T01:CDS | 45.0% | |
| CCATAGACAGTGTAGAAGCT+GGG | + | Chr1:9265414-9265433 | None:intergenic | 45.0% | |
| GACCTTGGGGATTTATGTCT+AGG | + | Chr1:9265063-9265082 | None:intergenic | 45.0% | |
| GTCCTAGACATAAATCCCCA+AGG | - | Chr1:9265058-9265077 | MsG0180000654.01.T01:CDS | 45.0% | |
| TCCATAGACAGTGTAGAAGC+TGG | + | Chr1:9265415-9265434 | None:intergenic | 45.0% | |
| CCACAAGTGTAATTGCAGCC+AGG | + | Chr1:9264905-9264924 | None:intergenic | 50.0% | |
| CCCAGCTTCTACACTGTCTA+TGG | - | Chr1:9265411-9265430 | MsG0180000654.01.T01:CDS | 50.0% | |
| CCTGGCTGCAATTACACTTG+TGG | - | Chr1:9264902-9264921 | MsG0180000654.01.T01:CDS | 50.0% | |
| GGCTGCAATTACACTTGTGG+AGG | - | Chr1:9264905-9264924 | MsG0180000654.01.T01:CDS | 50.0% | |
| TCCGTAACTGTCGCAACCAA+CGG | + | Chr1:9265215-9265234 | None:intergenic | 50.0% | |
| ! | ACCGTTGGTTGCGACAGTTA+CGG | - | Chr1:9265211-9265230 | MsG0180000654.01.T01:CDS | 50.0% |
| !! | CAGCCAGGTAGAACTTGTTG+AGG | + | Chr1:9264890-9264909 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 9264827 | 9265489 | 9264827 | ID=MsG0180000654.01;Name=MsG0180000654.01 |
| Chr1 | mRNA | 9264827 | 9265489 | 9264827 | ID=MsG0180000654.01.T01;Parent=MsG0180000654.01;Name=MsG0180000654.01.T01;_AED=0.44;_eAED=0.44;_QI=0|-1|0|1|-1|1|1|0|220 |
| Chr1 | exon | 9264827 | 9265489 | 9264827 | ID=MsG0180000654.01.T01:exon:1959;Parent=MsG0180000654.01.T01 |
| Chr1 | CDS | 9264827 | 9265489 | 9264827 | ID=MsG0180000654.01.T01:cds;Parent=MsG0180000654.01.T01 |
| Gene Sequence |
| Protein sequence |
